WO2018225884A1

WO2018225884A1 - Sample collection container assembly comprising pipette

Info

Publication number: WO2018225884A1
Application number: PCT/KR2017/005974
Authority: WO
Inventors: 이은혜
Original assignee: (주)알엠바이오
Priority date: 2017-06-08
Filing date: 2017-06-08
Publication date: 2018-12-13

Abstract

The present invention relates to an improved sample collection container assembly comprising a pipette, the container assembly enabling the prevention of cellular degeneration, the prevention of a loss of cells during transportation or storage, and the obtaining of a uniform distribution of cell circles on a membrane filter, thereby increasing test efficiency and enabling a test to be accurately conducted.

Description

Sampling Container Assembly with Pipette

The present invention relates to a sample collection container assembly including a pipette, and in particular, it is possible to easily collect a sample (sample cell) from a sample container, to prevent denaturation of cells during collection and to prevent cell loss during transportation or storage. In addition, the present invention relates to a sampling container assembly including an improved pipette to obtain a uniform distribution of cell circles in a membrane filter, thereby improving inspection efficiency and making accurate inspection.

Pap smear is a method of observing the collected cells under a microscope to determine whether the collected cells are infected, whether there is inflammation, abnormal cells or cancer cells.

Pap smears are widely used in the diagnosis of cervical cancer by observing cells collected from the tissues or secretions of the cervix to confirm the presence of abnormal cells. Applied to cell culture.

Pap smearing is mainly performed by fixing the cells contained in the collected sample, collecting the cells, smearing them on a slide, and reading them under a microscope or the like.

An example sample container is disclosed in Korean Patent Publication No. 10-2005-0111724 and shown in FIGS. 1 and 2.

This advances the piston 22 to inject the cells C precipitated in the cell fixation chamber 211 from the cell fixation chamber 211 into the cell harvesting chamber 212 and to plate the cells directly into the stained glass G. It is provided with a configuration in which the cells (C) is adsorbed to the sampling chamber cap (23) so as to be able to.

The sampling chamber cap 23 includes a filter coupling part 232 having a filter 236 attached to an upper end thereof, and a space formed inside the filter coupling part 232 to adsorb the cells C to the filter 236 ( An inlet 234 may be formed to inhale a fluid such as air from the 233, and as shown in FIG. 2, the cells C adsorbed to the filter 236 may be detached from the surface of the stained glass G when smeared. In order to inject a fluid such as air into the space 233 formed inside the filter coupling portion 232 to be formed is formed.

On the other hand, in the cell collection chamber 212, a strainer 24 for filtering large particles is provided between the injection port 213 and the filter 236 of the collection chamber cap 23.

Such a sample container has an advantage that the fixation and collection of cells is made in one container, so that the fixation and collection of cells are easy and the possibility of contamination of the cells is reduced in the process.

By the way, the sample container of the above configuration has the following problems.

First, when the cell C moves from the cell fixing chamber 211 to the cell collecting chamber 212, the cell C passes through the gap between the injection port 213 and the injection piston part 222 by the pressure of the piston 22. Degeneration of (C) takes place, making accurate inspection difficult.

Second, since the piston 22 is transported and stored in a state in which the piston 22 is coupled to the container body 21, there is a problem in that the piston 22 is undesirably detached and the collected cells C are lost.

Third, the central portion of the filter 236 due to the injection piston portion 222

The present invention was created in order to solve the above problems, sample collection containing a pipette to simplify the collection of the sample from the sample container, to prevent the denaturation of cells and to prevent the loss of cells during transport or storage It is an object to provide a container assembly for use.

It is another object of the present invention to provide a sampling container assembly including an improved pipette to obtain a uniform distribution of cell circles in a membrane filter, thereby increasing test efficiency and making accurate test.

The container assembly for sampling containing the present invention pipette to achieve the above object is a container body is formed with a pipette for accommodating the solution solution and the collected cells, the top is opened and the solution solution and cells are discharged at the bottom, the container body A first cap member detachably coupled to an upper end of the first cap member to open and close the opening, and a second cap member coupled to a lower end of the container body and having a hollow guide tube protruding inwardly at a position opposite the pipette; A container attached to an upper end of the guide tube and having a first sealing film in contact with an end of the pipette;

A bottom plate which is guided to the guide tube and penetrates the first sealing film and the pipette enters the upper part and protrudes upward in the center portion, and is attached to an end of the pipe and breaks when the pipette enters. A connecting member including a sealing film, an upper coupling part extending upwardly from an edge of the bottom plate to be detachably coupled to the second cap member, and a lower coupling part extending downward from an edge of the bottom plate;

A cylindrical shape having a middle plate having a suction hole formed in the middle portion and having an upper end detachably coupled to the lower coupling portion and having a vacuum suction unit connected to the lower portion of the middle plate, and a membrane filter attached to the upper end of the body. With filter fixing body,

Solution solution and cells in the container is characterized in that it is guided to the lower coupling side through the pipette and the fusion pipe.

In addition, the present invention is characterized in that the lower end of the container body is inclined surface of the upper and lower narrow is formed, the pipette is formed at the lower end of the inclined surface.

In addition, the present invention is characterized in that the guide of the upper and lower strait is formed on the lower surface of the bottom plate, the cells guided to the fusion pipe is guided to the central portion of the membrane filter.

In addition, the present invention is characterized in that the filter member for filtering the suspended solids in the solution solution inside the fusion pipe is further provided.

In addition, the present invention is characterized in that the cushion body for passing through the solution solution between the membrane filter and the intermediate plate to support the membrane filter is further provided.

In addition, the present invention is characterized in that the inner rib is further formed on the inner side of the second cap member in close contact with the inclined surface.

First, unlike the conventionally collected cells by the piston, the container assembly of the present invention is a pipette 112 is entered into the fusion pipe 54 to extract the cells in the container 100, so that the cells can be easily collected and the denaturation of the cells. To prevent.

Second, unlike the prior art, there is a fear of cell loss due to the detachment of the piston container assembly of the present invention to prevent the loss of cells during transportation or storage by the combination of the cap member (120).

Third, the container assembly of the present invention is provided with a guide 55 of the upper and lower narrow narrow to obtain a cell circle with a uniform distribution in the membrane filter 63 to increase the test efficiency and to make an accurate test.

Fourth, by providing the cushioning body 64 in the filter fixing body 60, the membrane filter 63 is provided with a cushion when it comes into contact with the slide 300 so that the cells C are easily transferred to and attached to the slide 300. It is possible to prevent the degeneration of the cells (C) by the contact pressure on the slide (300).

1 is a cross-sectional view showing a conventional sample container structure,

FIG. 2 is a schematic view showing a state in which cells are stained with stained glass G from the structure of FIG. 1; FIG.

3 is an exploded cross-sectional view showing a container assembly of an embodiment of the present invention;

4 is a cross-sectional view of the combination of FIG.

5 is a schematic view showing a state of smearing cells on a slide;

6 is a schematic view showing a state in which cells are plated in a slide on a slide.

Pipette (sample) container assembly is formed of the embodiment of the present invention to simplify the collection of cells and prevent denaturation, to prevent the loss of cells during transport or storage, and to provide a uniform distribution of cell circles on the membrane filter So that inspection efficiency can be improved and accurate inspection can be made.

3 and 4 showing a sample container assembly according to an embodiment of the present invention, which forms a container 100 in which a solution solution and collected cells are accommodated, and a solution passage in the container 100 and a cell passageway. A connector 50 and a filter fixture 60 separating the solution solution and the cells moved through the connector 50 are provided.

The container 100 includes a container body 110 in which a solution solution and the collected cells are accommodated and an upper end thereof is opened and a pipette 112 is formed at the bottom of which the solution solution and the cells are discharged, and an upper end of the container body 110. Removably coupled to the first cap member 120 for opening and closing the opening, and a hollow guide tube coupled to the lower end of the container body 110 and protruded inward at a position opposite to the pipette 112 ( A second cap member 130 having a 142, and a first sealing film 141 is attached to the upper end of the guide tube 142 in contact with the end of the pipette 112.

The lower end of the container body 110 is formed with an inclined surface 111 of the upper and lower strait, the pipette 112 is formed at the lower end of the inclined surface 111, the discharge of the solution solution and cells contained in the container body 110 This is supposed to be what you want.

An inner rib 132 having an upper end in close contact with the inclined surface 111 is formed inside the second cap member 130, so that the solution leaks from the container matrix 110 despite the first sealing film 141. Prevent external leakage of solution and cells.

The first sealing film 141 is applied to a thin plate such as silver foil, so that it can be easily torn by the fusion pipe (54).

The connecting member 50 is guided to the guide tube 142 and penetrates the first sealing film 141 and the fusion pipe 54 into which the pipette 112 enters is projected upward in the center portion. The bottom plate 53 formed thereon, the second sealing film 56 adhered to an end of the fusion pipe 54, and broken when the pipette 112 enters, and extends upward from an edge of the bottom plate 53. And an upper coupling part 51 detachably coupled to the second cap member 130 and a lower coupling part 52 extending downward from an edge of the bottom plate 53.

The second sealing film 56 is formed of a silicon material, so that the elastic second sealing film 56 adheres to the outer circumferential surface of the pipette 56 when the pipette 112 penetrates the second sealing film 56. To prevent external leakage of solution solution and cells.

The lower surface of the bottom plate 53 is provided with a guide 55 of the upper and lower narrow, so that the cells (C) guided to the fusion pipe 54 is guided to the central portion of the membrane filter (63).

Further, a filter member 57 is provided inside the fusion pipe 54 for filtering the suspended matter in the solution solution.

Reference numeral 58 is a first flange formed at the boundary between the upper coupling portion 51 and the lower coupling portion 52.

The filter fixing body 60 is a tubular shape and has an intermediate plate 61 having a suction hole 61a formed at an intermediate portion thereof, and an upper end thereof is detachably coupled to the lower coupling portion 52 and has a lower portion of the intermediate plate 61. And a body 65 to which the vacuum inhaler 400 is coupled, and a membrane filter 63 attached to an upper end of the body 65.

A cushioning body 64 is provided between the membrane filter 63 and the intermediate plate 61 to pass the solution solution and support the membrane filter 63.

Reference numeral 65 is a second flange formed outside the body 62.

How to use the sample container assembly of the above configuration is as follows.

First, the solution solution and the cells are accommodated in the container body 110, and the first and

second cap members

120 and 130 are combined with each other, so that the second cap member 130 is located at the bottom as shown in FIG. .

At this time, the solution solution and cells (C) is not leaked from the container body 110 because the end of the pipette 112 is in contact with the first sealing film 133.

Subsequently, the coupling body 50 and the filter fixing body 60 are coupled to each other, and the vacuum inhaler 400 is mounted on the body 62 of the filter fixing body 60, as shown in FIG. 4. The pipette 112 enters the inside of the fusion pipe 54 while breaking the first and second sealing

films

133 and 56 by entering 54 into the guide tube 131.

At this time, the second sealing film 56 is broken by the pipette 112, and the first sealing film 133 is damaged by the fusion pipe 54.

After the assembly is completed, the solution solution and the cells in the container 100 are drawn by the suction force of the vacuum inhaler 400 to the inside of the lower coupling portion 52 of the connecting member 50 through the pipette 112 and the fusion pipe 54. The solution solution is discharged through the suction hole (61a) of the intermediate plate 61 and the cell (C) is filtered on the upper surface of the membrane filter (63).

At this time, a large float in the solution solution is filtered by the filter member (57).

On the other hand, the cell (C) is concentrated in the center portion of the membrane filter 63 by the guide 55 as shown in Figure 4 and 6 will form a circle as the cells overlap.

In this case, as the vacuum suction force is transmitted through the vacuum inhaler 400, the adherence of the sample cells to the slide 300 may be achieved even if the center portions of the cushioning body 64 and the membrane filter 63 are curved concave downward. Has the advantage to improve.

Subsequently, the operator detaches the filter holder 60 and then contacts the membrane filter 63 to the slide 300, as shown in FIG. 5, thereby bringing the sample cells C to the surface of the slide 300. Attach.

At this time, the cushioning body 64 provides a cushion when the membrane filter 63 is in contact with the slide 300 so that the cells C can be easily transferred and attached to the slide 300, and the slide 300 is provided with respect to the slide 300. The contact pressure prevents the cell C from being denatured.

These sample cells are attached to the surface of the slide 300 in the form of a circular cell circle has the advantage of improving the cell analysis efficiency.

In the container assembly of the present invention as described above, the pipette 112 enters the fusion pipe 54 and extracts the cells, unlike the conventional one, in which the cells are collected by the piston, thereby preventing denaturation of the cells.

In addition, the container assembly of the present invention can prevent the loss of cells during transportation or storage by the combination of the first and

second cap members

120 and 130, unlike the conventional piston, which is concerned about cell loss due to detachment of the piston. .

In addition, the container assembly of the present invention can obtain a cell circle with a uniform distribution in the membrane filter 63 to increase the inspection efficiency and to make accurate inspection.

The present invention relates to a sample collection container assembly including a pipette, which can easily collect a sample (sample cell) from a sample container, prevent degeneration of cells during collection, and prevent loss of cells during transportation or storage. It is possible to obtain a uniform distribution of cell circles in the membrane filter to increase the inspection efficiency and to make accurate inspections.

Claims

The solution solution and the collected cells are accommodated and the container body 110 is formed with a pipette 112 for opening the upper end and the solution solution and the cell is discharged at the bottom, and detachably coupled to the upper end of the container body 110 A second cap member 120 which opens and closes the opening, and a hollow guide tube 142 which is coupled to the lower end of the container body 110 and protrudes inwardly at a position opposite to the pipette 112. A container (100) having a cap member (130) and a first sealing film (141) attached to an upper end of the guide tube (142) and in contact with an end of the pipette (112);

A bottom plate 53 formed to be guided to the guide tube 142 to penetrate the first sealing film 141 and to have the fusion pipe 54 protruding upward in a central portion thereof through which the pipette 112 enters the inside; And a second sealing film 56 adhered to an end of the fusion pipe 54 and broken when the pipette 112 enters, and extends upwardly from an edge of the bottom plate 53 to the second cap member 130. A coupling member (50) having an upper coupling portion (51) detachably coupled to the lower portion) and a lower coupling portion (52) extending downward from an edge of the bottom plate (53);

It has a cylindrical shape and has an intermediate plate 61 having a suction hole 61a formed at an intermediate portion thereof, and an upper end thereof is detachably coupled to the lower coupling portion 52, and a vacuum suction unit 400 is connected to the lower portion of the intermediate plate 61. And a filter fixing body 60 having a body 62 and a membrane filter 63 attached to an upper end of the body 62.

The sample assembly container assembly containing a pipette, characterized in that the solution solution and the cells in the container 100 is guided to the lower coupling portion 52 through the pipette 112 and the fusion pipe (54).
The sample collection including the pipette according to claim 1, wherein the lower end of the container body 110 is formed with an inclined surface 111 of an upper and lower narrow strait, and the pipette 112 is formed at the lowermost end of the inclined surface 111. Container assembly for
The method of claim 1 or 2, the upper surface of the lower plate 53 is formed with a guide 55 of the upper and lower narrow, so that the cells guided to the fusion pipe 54 is guided to the central portion of the membrane filter 63. Sampling vessel assembly comprising a pipette, characterized in that.
The sample assembly container assembly according to claim 1 or 2, further comprising a filter member (57) for filtering the suspended matter in the solution solution inside the fusion pipe (54).
The method according to claim 1 or 2, characterized in that the cushion body 64 for passing through the solution solution between the membrane filter 63 and the intermediate plate 61 to support the membrane filter 63 is further provided. Sampling vessel assembly comprising a pipette to.
The sample assembly container assembly of claim 2, wherein an inner rib (132) having an upper end in close contact with the inclined surface (111) is further formed inside the second cap member (130).