KR20090079487A - Sample container for fixing and extracting cells - Google Patents

Abstract

A sample container for fixing and collecting cells is provided to easily inject the cells from fixation room to collecting room. A sample container(10) for fixing and collecting cells comprises a container main body(11), injection rod(12), fixation room cap(13) of disk form, and collection room cap(14). The container main body comprises cell fixation room(111), cell collection room(112), and injection port(113). The cell fixation room comprises cylindrical body(111a) and cone portion(111b). The cell collection room comprises a precipitation part(112a), hook end(112b), cylindrical body(112c). The fixation room cap has inserting groove(131) and rod fixing cap(132). The fixation room cap is inserted at the entrance of cell fixation room. The collection cap comprises cylindrical body(141), membrane equipment part(142) and membrane(143). The collection room cap is inserted at the entrance of cell collection room.

Description

Sample container for cell fixation and collection {SAMPLE CONTAINER FOR FIXING AND EXTRACTING CELLS}

The present invention relates to a sample container for cell fixation and sampling, and more particularly, to a cell container for sample fixation and sampling that can be easily fixed and collected by the fixed solution.

Pap smear is a method of observing collected cells under a microscope to check whether the collected cells are infected, inflamed, abnormal cells or cancer cells. Pap smears are widely used in the diagnosis of cervical cancer, which checks for abnormal cells by observing cells collected from tissues or secretions of the cervix, which are clinical specimens or tissues such as sputum, urine, exudates, cerebrospinal fluid and fine needle inhalation tests. Applied to cell culture.

Pap smearing is mainly performed by fixing the cells contained in the collected sample, collecting the cells, smearing them on a slide, and reading them under a microscope or the like.

Samples taken from the body are placed in containers for transport, cell fixation and collection. In the related art, generally, a sample is transferred from a container containing a sample collected for cell fixation and collection to a cell fixation container to fix a cell and collect fixed cells. This process involves the transfer of samples and cells across several vessels, which is very cumbersome and has the problem of cell contamination in the process.

In order to solve the above problems, the inventors of the present invention do not need to transfer the sample and cells in the process of fixing and collecting the cells contained in the sample collected in the Republic of Korea Patent Publication No. 10-2005-0111724 A sample container for cell fixation and harvesting is presented which is convenient and free of cell contamination. 5 is a view showing a sample cell 20 for fixing and collecting a conventional cell disclosed in the Republic of Korea Patent Publication No. 10-2005-0111724. Referring to the drawings, the conventional cell fixation and collection sample container 20 is composed of a container body 21, a piston 22 and the sampling chamber cap 23. The container body 21 has a cylindrical piston slide portion 211a having an inlet at an upper portion thereof, a cell fixing chamber 211 provided with a cone portion 211b at a lower portion thereof, and a lower portion of the cell fixing chamber 211. A cell collection chamber 212 formed to be separated from the cell fixing chamber 211 and having an inlet at a lower portion thereof, and having a circular shape and extending in a length direction of the piston slide portion 211a of the cell fixing chamber 211. The injection port 213 is formed to lead from the cell fixing chamber 211 to the cell collecting chamber 213. The piston 22 has a pressurized piston portion 221 formed in a cylindrical shape such that the piston 22 is inserted into the inlet of the cell fixing chamber 211 so that an outer surface thereof slides in close contact with the inner surface of the piston slide portion 211a. A cone portion 223 is formed at the lower portion of the portion 221 to correspond to the cone portion 211a of the cell fixing chamber 211, and protrudes downward from the lower portion of the cone portion 223 to have a cylindrical shape. An injection piston portion 222 extending in length may be inserted into the injection hole 213 to close the injection hole 213 to be in close contact with the inner surface of the injection hole 213 and the contact portion of the injection hole 213. It is formed to have a gap portion spaced apart from the inner surface of the injection hole so that a gap is formed between the inner surface. The capping chamber cap 23 is to block the inlet of the cell collection chamber 212 of the container body 21 so that the capping chamber 23 can smear the cells directly on stained glass (not shown). It is composed. That is, the sampling chamber cap 23 is formed in the filter coupling portion 232 and the filter coupling portion 232 extending upwardly protruding in an annular shape from the portion exposed into the cell collection chamber 212. A filter 236 having an edge coupled to the filter coupling part 232 is provided to cover the space 233. The height of the filter coupling portion 232 is formed higher than the height of the screw insertion portion 231 screwed to the sampling chamber cap screw insertion portion 215 formed around the inlet of the cell collection chamber 212. In addition, the collection chamber cap 23 has a suction port for sucking a fluid such as air from the space 233 formed inside the filter coupling portion 232 so that the cells C are adsorbed to the filter 236 ( 234 is formed, and the air (C) to the space 233 formed inside the filter coupling portion 232 to detach the cells (C) adsorbed on the filter 236 on the surface of the stained glass (G) An injection hole 235 may be formed to inject the same fluid. On the other hand, in the cell collection chamber is provided with a strainer 24 for filtering a large lump of particles between the injection port 213 and the filter 236 of the collection chamber cap 23.

In the conventional cell fixation and collection sample container 20 having the above configuration, since the fixation and collection of the cells are made in one container, the fixation and collection of the cells are easy and the possibility of contamination of the cells in the process is reduced. In addition, the cells can be adsorbed onto the filter to allow direct staining of the stained glass, thereby facilitating collection and smearing of the cells.

 However, the conventional cell fixation and sampling sample vessel 20 as described above is a cell fixation chamber in the process of injecting the cells and the fixed solution contained in the cell fixation chamber 211 to the cell collection chamber 212 by pushing the piston 22 It is difficult to push the piston 22 because the pressure is formed on the 211, and the collecting chamber cap 23 is screw-fastened to the container main body 21. In order to automate using, the collection chamber cap 23 is inconvenient to be separated from the container body 21 and introduced into the smearing equipment.

The present invention is to solve the above problems, the object of the present invention is to fix the cells contained in the sample collected and do not need to transfer the sample and cells in the process of collecting the convenient use and cells will be contaminated In addition, there is no concern, and in the process of injecting the cells from the fixed chamber to the collection chamber, no pressure is formed in the fixed chamber, so that the injection is easy and the collection cap is inserted into the container body, so the collection cap is easily separated from the container body. It is to provide a sample container for cell fixation and harvesting to utilize the smearing device.

In order to achieve the above object, the cell fixation and sample collection container according to the present invention, in the cell fixed and sample collection container for fixing the cells contained in the sample collected and the fixed cells, the upper portion, A cylindrical cylinder having an inlet formed therein, and a cell anchoring chamber formed at a lower portion of the cylindrical section with a cone portion narrower toward the lower side of the upper and lower straits, and separated from the cell anchoring chamber by the cone at the lower portion of the cell anchoring chamber and the cone A cell sampling chamber having a settling portion provided around the apex of the portion, the cylindrical portion extending downward from the settling portion to have an inlet at the lower portion, and an annular hooking end formed between the settling portion and the cylindrical portion, and the lowest apex of the cone portion; Extend a predetermined length downward from the cell fixation chamber to the cell collection chamber. A close contact portion of the container body with an injection hole formed therein, and an injection portion inserted into the entrance of the cell fixing chamber and extending from the lower end in a cylindrical shape to a predetermined length so as to be in close contact with the inner surface of the injection hole so as to block the injection hole; It is formed to have a gap spaced apart from the inner surface of the injection hole so that a gap is formed between the contact portion and the inner surface of the injection hole, and is inserted into the cylindrical portion of the cell fixing chamber between both ends and fixed inside the cylinder portion. An injection rod having a disk-shaped guide part having a distribution hole formed up and down, and an insertion hole is formed at the center thereof, and inserted into the insertion hole to block the insertion hole and slide in the vertical direction, and the upper end of the injection rod is inserted into and coupled to the lower part. Rod fixing cap is provided with a coupling groove for forming the cell to block the inlet of the cell fixing chamber A disk-shaped fixing chamber cap which is inserted into and fixed to the entrance of the chamber, and a cylindrical portion inserted into and adhered to the cylindrical portion of the cell collection chamber, and a membrane mounting portion is formed at an upper end of the cylindrical portion, and a membrane is mounted on the membrane mounting portion. It is characterized in that consisting of the cap of the capping chamber is inserted into the inlet of the cell collection chamber to block the inlet of the cell collection chamber.

In addition, the cell container for collecting and collecting the sample according to the present invention, the contact portion of the injection portion is at least one or more O-ring provided in an annular shape in the injection piston portion, the gap portion of the injection portion is formed between the O-ring It is characterized by a gap.

By the above configuration, the cell fixing and collecting container according to the present invention does not need to move the sample and cells in the process of fixing and collecting the cells contained in the collected sample, which is convenient to use and the cells may be contaminated. In addition, there is no pressure in the fixing chamber in the process of injecting the cells from the fixing chamber into the collecting chamber, so that the injection cap is easy and the collecting cap is inserted into the container body. It has the advantage of being able to utilize the equipment.

Hereinafter, with reference to the embodiment shown in the drawings will be described in more detail the sample container for capturing and collecting according to the present invention.

1 is a perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention, Figure 2 is an exploded perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention, 3 is a cutaway perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention, Figures 4a to 4c shows a method of using a sample container for cell fixation and collection according to an embodiment of the present invention One cross section.

The cell container for fixation and collection according to the present invention is to collect and fix the cells contained in the sample collected in a fixed solution and observe the fixed cells. Referring to the drawings, one embodiment of the present invention According to the cell holding and sampling sample container 10 is composed of a container body 11, the injection rod 12, the fixed chamber cap 13 and the collection chamber cap (14).

The container body 11 is an injection port 113 formed therebetween to connect the cell fixing chamber 111, the cell collecting chamber 112, and the cell fixing chamber 111 and the cell collecting chamber 112 separated from each other. It is formed to have.

The cell fixing chamber 111 accommodates a fixed solution for cell fixation therein, and contains a sample taken from the human body in the fixed solution, whereby the cell is fixed over a certain period of time while the cell (C) is another part of the sample such as a body fluid. It is a space for sedimentation separately from. Referring to the drawings, the cell fixing chamber 111 is provided with a cylindrical cylindrical portion (111a) for inserting the guide portion 121 of the injection rod 12. An upper portion of the cylindrical portion 111a is provided with an inlet, and the fixing chamber cap 13 is inserted into and fixed to the inlet so as to be blocked.

The lower portion of the cylindrical portion (111a) of the cell fixing chamber 111 is formed with a cone portion (111b) for collecting the precipitated cells (C). That is, the cone portion (111b) is located in the lower portion of the cylindrical portion (111a) is formed gradually narrower toward the lower side of the ordinary light, and thus the precipitated cells (C) at the lower apex of the cone portion (111b) It is gathered to the injection hole 113 formed. The cells (C) that are fixed as described above and gathered into the injection holes 113 formed at the apex of the cone part 111b are collected through the injection holes 113 by the operation of the injection rod 12. 112).

The cell collection chamber 112 is separated from the cell fixing chamber 111 to receive a fixed solution therein, and the cells C through the injection port 113 connected to the cell fixing chamber 111 into the fixed solution. Is injected. The cells C injected into the cell collection chamber 112 are collected together with the fixed solution contained in the cell collection chamber 112 or only in the precipitated state by a sampling device P such as a pipette or the membrane 143. Is adsorbed on. Referring to the drawings, the cell collection chamber 112 is separated from the cell fixing chamber 111 at the lower portion of the cell fixing chamber 111. In the cell collection chamber 112, a precipitation portion 112a for collecting and gathering the cells C inside is formed in an annular recess around the cone portion 111b of the cell fixing chamber 111, and the precipitation portion The cylindrical part 112c extends downward from 112a, and is formed so that it may have an inlet in the lower part. An annular locking end 112b is formed between the settling portion 112a of the cell collection chamber 112 and the cylindrical portion 112c. The inlet of the cylindrical portion 112c of the cell collection chamber 112 is sealed to be opened and closed by the collection chamber cap 14. According to the present invention, since the cylindrical portion 141 of the capping chamber 14 is inserted into the cylindrical portion 141 of the cell capturing chamber 112 and slides, the capping chamber 14 of the capping chamber 14 may be moved using the device. It becomes easy to separate from the main body 11.

The injection port 113 is a passage for moving the cell C fixed and precipitated in the cell fixing chamber 111 to the cell collection chamber 112. Referring to the drawings, the injection port 113 is formed at the lowermost apex of the conical portion 111b of the cell fixing chamber 111 to extend a predetermined length in the direction of the main body 11, the cell fixing chamber ( 111 is formed to lead to the cell collection chamber (112).

The injection rod 12 blocks the injection hole 113 in a process of fixing and precipitating the cells C of the sample introduced into the cell fixing chamber 111, and slides and moves the precipitated cells C to the injection hole. Injecting the cell collection chamber 112 from the cell fixing chamber 111 through the 113. Referring to the drawings, the injection rod 12 is composed of a guide portion 121, the injection portion 122. A disk-shaped guide portion 121 is provided between both ends of the injection rod 12 to be inserted into and fixed to the cylindrical portion 111a of the cell fixing chamber 111. The guide part 121 is provided with a distribution hole 121a for distributing the cells C and the fixed solution introduced into the cell fixing chamber 111 up and down. The guide portion 121 is inserted into the cylindrical portion 111a of the cell fixing chamber 111. The lower end of the injection rod 12 is in close contact with the inner surface of the injection hole 113 is inserted into the injection hole (122) extending in a predetermined length in a cylindrical shape to block the injection hole 113 It is formed to have a gap spaced apart from the inner surface of the injection hole 113 so that a gap (not shown) is formed between the (122a) and the close contact portion (122a) and the inner surface of the injection hole (113). The close contact portion 122a of the injection portion 122 is configured by installing an O-ring on the injection portion 122 for sealing between the outer surface of the injection portion 122 and the inner surface of the injection hole 113. A portion other than the O-ring, which is the close contact portion 122a of the injection portion 122, is formed with a gap between the inner surface of the injection hole 113, and through the gap, the cell (C) is a cell fixing chamber ( 111 is moved to the cell collection chamber 112. That is, the injection hole 113 is blocked by the O-ring, which is the contact portion 122a of the injection portion 122, and when the injection rod 12 is moved in the longitudinal direction of the main body 11, the contact portion As the O-ring (122a) is released below the injection hole 113, the injection hole 113 is opened, and accordingly, the cell C is connected to the cell fixing chamber through the gap portion of the injection part 122. 111 is injected into the cell collection chamber 112.

The fixing chamber cap 13 is configured to block the upper inlet of the cylindrical portion 111a of the cell fixing chamber 111 and to insert and combine the upper end of the injection rod 12. The fixing chamber cap 13 is formed in a substantially disk shape, and an annular indentation groove 133 is formed along an edge thereof, and the upper inlet of the cylindrical portion 111a of the cell fixing chamber 111 is formed in the indentation groove 133. Is inserted into and engaged with each other. Referring to the drawings, the indentation groove 133 is opened downwardly between the side walls 133a and 133b formed by continuously bending the outer edge of the fixing seal cap 13 to form an annular shape.

On the other hand, the upper end of the injection rod 12 is inserted into the cell fixing chamber 111 in the center of the fixing chamber cap 13 is coupled to be slidable in the vertical direction. To this end, an insertion hole 131 is formed in the center of the fixing chamber cap 13, and a rod fixing cap 132 for coupling the upper end of the injection rod 12 to the insertion hole 131 in the vertical direction. Slidably inserted. Referring to the drawings, the edge of the insertion hole 131 is bent downward and the sliding support 131a extending downward is formed in an annular shape. The rod fixing cap 132 inserted into the insertion hole 131 to be slid in the vertical direction is provided with a protrusion 132a which is surrounded by the sliding support 131a and inserted into the insertion hole 131, and the protrusion 132a. The upper end of the injection rod 12 is inserted into the lower end of the coupling groove 132c is formed to be coupled, the inner edge of the end wall 132b formed by bending the peripheral edge of the coupling groove 132c upwards and the A sliding groove 132d is formed between the outside of the protrusion 132a. In the rod fixing cap 132 formed as described above, the protrusion 132a is surrounded by the sliding support 131a and inserted into the insertion hole 131, so that the sliding support 131a is inserted into the sliding groove 132d. . As described above, the upper end of the injection rod 12 is inserted into and coupled to the coupling groove 132c of the rod fixing cap 132 inserted into the insertion hole 131. On the other hand, the rod fixing cap 132 is inserted into the insertion hole 131 so that the upper surface of the protrusion 132a is exposed to the upper portion of the fixing chamber cap 13, the user is a protrusion of the rod fixing cap 132 Pressing the upper surface of the (132a) the rod fixing cap 132 is slid to the lower side of the insertion hole 131, accordingly the injection rod 12 is coupled to the rod fixing cap 132 the upper end is downward Will be moved.

The collecting chamber cap 14 is configured to block the inlet of the cell collecting chamber 112 and to adsorb the cells moved to the cell collecting chamber 112.

Referring to the drawings, the collection chamber cap 14 is provided with a cylindrical portion 141 that is inserted into and adhered to the cylindrical portion 122c of the cell collection chamber 112, and the cylindrical portion of the collection chamber cap 14 The membrane mounting portion 142 is formed at the upper end of the 141. The membrane 143 is mounted on the membrane mounting part 142 to filter and filter the filtered cells C while passing through the fixing solution. Meanwhile, between the cylindrical portion 141 of the capping chamber cap 14 and the membrane mounting portion 142, a hook for catching the locking end 112b of the cell collection chamber 112 around the membrane mounting portion 142. Step 144 is formed in an annular shape.

The sampling chamber cap 14 formed as described above is inserted into the cylindrical portion 141 to be in close contact with the cylindrical portion 112c of the cell collecting chamber 112 to block the entrance of the cell collecting chamber 112. Coupled to (11). On the other hand, according to the present invention, the cylindrical cap 141 is provided in the capping chamber 14, and the cylindrical cap 141 is inserted into the cylindrical cap 112c of the cell capturing chamber 112. Pressing and pulling the sampling chamber cap 14 has a structure in which the sampling chamber cap 14 is easily inserted into and removed from the cell collection chamber 112.

4A to 4D are cross-sectional views illustrating a method of operating and using the cell container for holding and collecting the sample container 10 according to an embodiment of the present invention. Hereinafter, the operation and use of the present invention will be described with reference to FIGS. 4A to 4D. The method will be described.

4A shows that the cells C and the fixative are accommodated inside the cell fixing chamber 111 of the main body 11 while the injection rod 12 is inserted to block the injection port 113 of the main body 11. The state is shown. Referring to FIG. 4A, the cell C injected into the cell fixing chamber 111 is collected and precipitated at the lower apex of the conical portion 111b of the cell fixing chamber 111, and the close contact portion of the injection rod 12 is fixed. O-ring (122a) is blocking the inlet 113 so that the precipitated cells (C) are not moved to the cell collection chamber (112).

4B illustrates a state in which cells C are injected into the cell collection chamber 112 from the cell fixing chamber 111 by opening the injection port 113 by moving the injection rod 12. Referring to FIG. 4B, by pressing the rod fixing cap 132 and sliding the rod fixing cap 132 downwardly from the insertion hole 131, a substantial portion of the rod fixing cap 132 is inserted into the rod fixing cap 132. While the injection rod 12 is moved downward, the contact portion 122a of the injection rod 12 is moved downward of the injection hole 113 so that the injection hole 113 is opened. When the injection hole 113 is opened, the cells C precipitated at the lower apex portion of the cone portion 111c of the cell fixing chamber 111 are injected into the injection hole 113 and the injection rod 12. It is moved to the cell collection chamber 112 through the gap between.

FIG. 4C illustrates a state in which the cells C moved to the cell collection chamber 112 are attached to the membrane 143 of the collection chamber cap 14. Cells C moved to the cell collection chamber 112 may be attached to the upper surface of the membrane 143 of the collection chamber cap 14 by precipitation. In addition, by generating a suction force from the lower end of the cap chamber 14, the fixed liquid is sucked from the cell collection chamber 112 and the fixed liquid is passed through the membrane 143 to filter the cells (C) to the membrane 143 Can be attached. The cells C attached to the membrane 143 as described above are plated in stained glass (not shown in the figure) for testing.

FIG. 4D illustrates a state in which the cells C moved to the cell collection chamber 112 are collected at the settling portion 112a of the cell collection chamber 112. As described above, the cells C collected in the sedimentation unit 112a of the cell collection chamber 112 can be collected using a dropper or a pipette (not shown).

The container for cell fixation and collection described above and shown in the drawings is only one embodiment for carrying out the present invention, and should not be construed as limiting the technical idea of the present invention. The scope of protection of the present invention is defined only by the matters set forth in the claims below, and the embodiments improved and changed without departing from the gist of the present invention will be apparent to those skilled in the art. It will be said to belong to the protection scope of the present invention.

1 is a perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention

Figure 2 is an exploded perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention

Figure 3 is a cutaway perspective view showing a sample container for cell fixation and collection according to an embodiment of the present invention

4A to 4C are cross-sectional views illustrating a method of using a sample container for cell fixation and collection according to an embodiment of the present invention.

Figure 5 is a cross-sectional view showing a sample container for conventional cell fixation and collection

<Short description of the major reference symbols>

10 Sample Container

     11 container body

          111 cell fixation chamber

               111a cylindrical part

               111b cone

          112 Cell Collection Room

               112a settling section

               112b hanging edge

               112c cylindrical part

          113 inlet

     12 injection rods

          121 guide

          122 injection section

               122a tight contact (O-ring)

          123 Cap insert

      13 Fixed Room Cap

          131 insertion hole

          132 rod fixing cap

      14 Harvest Cap

           141 Cylindrical section

           142 Membrane Mount

           143 membrane

           144 jam ends

Claims (2)

In the sample container for cell fixation and sampling for fixing the cells contained in the sample collected and collecting the fixed cells, A cylindrical cylinder having an inlet formed at an upper portion thereof, and a cell anchoring chamber formed at a lower portion of the cylindrical portion with a conical portion that becomes narrower toward the lower side of the upper and lower straits; A cell collection chamber having a settling portion provided around the apex of the conical portion, the cylindrical portion extending downward from the settling portion to have an inlet at the bottom thereof, and an annular hooking end formed between the settling portion and the cylindrical portion; A container body in which an injection hole is formed to extend from a lowermost vertex to a predetermined length and extend from the cell fixing chamber to the cell collecting chamber, The inner surface of the injection hole is inserted between the close contact portion and the close contact portion that is inserted into the inlet of the cell fixing chamber and extends a predetermined length in a cylindrical shape from the bottom to the injection hole to close the injection hole. Is formed to have a gap spaced apart from the inner surface of the injection hole so as to form a gap between the guide is inserted into the cylindrical portion of the cell fixing chamber between the two ends is fixed in the cylindrical portion of the disk-shaped guide hole formed up and down Injecting rod provided with, Insertion hole is formed in the center, the rod fixing cap is inserted into the insertion hole to block the insertion hole and slide in the vertical direction and the upper end of the injection rod is inserted in the lower portion is provided with a rod fixing cap for coupling, the cell fixed A disk-shaped fixation chamber cap inserted into and fixed to the inlet of the cell fixing chamber so as to block the inlet of the seal, A cylindrical portion is inserted into the cylindrical portion of the cell collection chamber and is in close contact with the cylindrical portion, and a membrane mounting portion is formed at the upper end of the cylindrical portion, and a membrane is mounted on the membrane mounting portion to block the entrance of the cell collection chamber. A sample container for cell fixation and collection, characterized in that consisting of a cap of the chamber is inserted into the inlet of the combination. The method of claim 1, The contact portion of the injection portion is at least one O-ring annularly installed in the injection piston portion, Cell injection and sample collection container, characterized in that the gap between the injection portion formed between the O-ring.

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