KR101825452B1 - A container assembly for picking free medical treatment included pipet - Google Patents

A container assembly for picking free medical treatment included pipet Download PDF

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Publication number
KR101825452B1
KR101825452B1 KR1020150175571A KR20150175571A KR101825452B1 KR 101825452 B1 KR101825452 B1 KR 101825452B1 KR 1020150175571 A KR1020150175571 A KR 1020150175571A KR 20150175571 A KR20150175571 A KR 20150175571A KR 101825452 B1 KR101825452 B1 KR 101825452B1
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KR
South Korea
Prior art keywords
container
cap member
cells
filter
engaging portion
Prior art date
Application number
KR1020150175571A
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Korean (ko)
Other versions
KR20170069314A (en
Inventor
김진오
한진석
김유순
Original Assignee
주식회사 인셀바이오
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Application filed by 주식회사 인셀바이오 filed Critical 주식회사 인셀바이오
Priority to KR1020150175571A priority Critical patent/KR101825452B1/en
Priority to PCT/KR2016/012282 priority patent/WO2017099357A1/en
Publication of KR20170069314A publication Critical patent/KR20170069314A/en
Application granted granted Critical
Publication of KR101825452B1 publication Critical patent/KR101825452B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C12M1/265—Pipettes; Syringes; Suction devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Abstract

Prevents denaturation of cells and prevents loss of cells during transportation and storage. It also enables to obtain uniformly distributed cell circles on the membrane filter to increase the efficiency of the test and to obtain samples with improved pipette To a container assembly.

Description

Technical Field [0001] The present invention relates to a container assembly for picking a pipette,

The present invention relates to a container assembly for sampling a sample containing a pipette, and in particular, it is possible to easily collect a sample (specimen cell) from a sample container and prevent denaturation of the cell during collection and prevent cell loss during transportation or storage The present invention also relates to a container assembly for sampling a sample containing an improved pipette for obtaining a uniform distribution of cell circles on a membrane filter to increase the inspection efficiency and to perform accurate inspection.

The cytological examination is a method of observing the collected cells with a microscope to see if the collected cells have infection, inflammation, abnormal cells or cancer cells.

The cytological examination is widely used for the diagnosis of cervical cancer which examines the cells collected from tissues or secretions of the cervix and confirms the presence of abnormal cells, and it is used for the diagnosis of cervical cancer such as sputum, urine, exudate, cerebrospinal fluid, Cell culture or the like.

The cytological examination is performed mainly by fixing the cells contained in the collected sample, collecting the cells, spreading them on a slide, and reading them with a microscope or the like.

An example of a sample container is disclosed in Korean Patent Laid-open Publication No. 10-2005-0111724 and shown in Figs.

This moves the piston 22 forward to inject the cells C settled in the cell fixing chamber 211 from the cell fixing chamber 211 to the cell collection chamber 212 and to directly apply the cells to the stain glass G The cells C are adsorbed on the sampling chamber cap 23 so that the cells C can be adsorbed.

The sampling chamber cap 23 is provided with a filter coupling portion 232 having a filter 236 at its upper end and a space formed inside the filter coupling portion 232 so that the cells C are attracted to the filter 236 The cell C adsorbed on the filter 236 can be desorbed on the surface of the stain glass G as shown in FIG. 2 by forming a suction port 234 capable of sucking a fluid such as air from the filter 236, An inlet 235 for injecting a fluid such as air into the space 233 formed in the filter coupling part 232 is formed.

On the other hand, in the cell collection chamber 212, a filtration net 24 is provided between the injection port 213 and the filter 236 of the collection chamber cap 23 in order to deposit a large lump of particles.

Such a sample container has advantages such that cell fixation and collection are performed in one container, so that cell fixation and collection are easy and the possibility of contamination of cells in the process is reduced.

However, the sample container having the above-described configuration has the following problems.

First, when the cell C moves from the cell fixing chamber 211 to the cell collection chamber 212, the cell C passes through the gap between the injection port 213 and the injection piston 222 by the pressure of the piston 22, (C) is deformed, and it becomes difficult to perform accurate inspection.

Secondly, since the container 22 is transported and stored in the state that the piston 22 is coupled to the container body 21, there is a problem that the collected cells C are lost due to the dislodgment of the piston 22.

Third, due to the injection piston portion 222, the cell density is relatively smaller in the central portion of the filter 236 than in the periphery thereof, which makes it difficult to inspect the efficiency and accuracy of the inspection.

Korean Patent Publication No. 10-2005-0111724

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide a sample collection device, And an object of the present invention is to provide a container assembly for a container.

 It is another object of the present invention to provide a container assembly for sample collection, which includes an improved pipette for obtaining a uniform distribution of cell circles on a membrane filter, thereby enhancing inspection efficiency and performing accurate inspection.

According to an aspect of the present invention, there is provided a container assembly for collecting a sample including a pipette, the container assembly comprising a solution body containing a solution solution and collected cells and having one end opened, a detachable coupling member for opening and closing the opening, A cap member having a through hole formed therein, and a flexible elastic plate coupled to the through hole;

A bottom plate formed to protrude upward at a center portion of the elastic tube penetrating the elastic plate and entering the inside of the container; an upper coupling portion extending upward from an edge of the bottom plate and detachably coupled to the cap member; And a lower coupling portion extending downward from an edge of the bottom plate;

And a membrane filter attached to the upper end of the body, wherein the membrane filter is attached to an upper end of the body, the upper end of the membrane filter being detachably coupled to the lower engaging portion and having a suction hole formed in a middle portion thereof, And a filter fixing body,

 And the solution solution and the cells in the container are guided to the side of the lower engaging portion through the melting orifice.

In addition, in the assembly of the present invention, a packing is attached to the outer circumferential surface of the cap member or the inner circumferential surface of the upper engaging portion so that airtightness is maintained when the cap member and the upper engaging portion are engaged.

Further, in the assembly of the present invention, the resilient plate is formed with a thin portion thinner than the periphery at the center thereof, and an entrance groove into which the tip of the lancing engine enters is formed.

In addition, an upper light guide is formed on the bottom surface of the bottom plate, and the cells guided by the trunk are guided to the center of the membrane filter.

The cap member may further include a filter member for filtering the suspended solids in the solution solution.

The membrane filter may further include a cushion body for passing the solution solution between the membrane filter and the intermediate plate to support the membrane filter.

First, unlike the case where the cells were collected by the conventional piston, the container assembly of the present invention allows the fibrillation tube 54 to enter the container 100 and take out the cells, so that the fibrillation can be easily taken and the cell degeneration can be prevented.

Secondly, unlike the prior art where there is a fear of cell loss due to the detachment of the piston, the container assembly of the present invention can prevent cell loss during transportation or storage by coupling of the cap member 120.

Third, the container assembly of the present invention includes the guide 55 for guiding the upper and lower light beams, so that the membrane filter 63 can obtain a uniform distribution of the cell circles, thereby enhancing the inspection efficiency and enabling accurate inspection.

Fourth, since the cushion body 64 is provided in the filter fixing body 60, the cushion is provided when the membrane filter 63 contacts the slide 300, so that the cell C is easily transferred to the slide 300, And prevents the cell C from being denatured by the contact pressure on the slide 300. [

Fifthly, the packing 125 is attached to the outer circumferential surface of the cap member 120 so that the airtightness is maintained when the cap member 120 and the upper engaging portion 51 are engaged. Therefore, the suction force by the inhaler 400 can be precisely Control.

1 is a sectional view showing a conventional sample container structure,
Fig. 2 is a schematic view showing a state in which cells are stained from the structure of Fig. 1 onto stain glass G,
3 is an exploded sectional view of a container assembly of an embodiment of the present invention,
Fig. 4 is an assembled cross-sectional view of Fig. 3,
5 is a cross-sectional view showing a filter fixture in which cells are filtered,
6 is a plan view showing a state where cells are filtered in a membrane filter,
7 is a schematic view showing a state in which cells are smudged on a glass.

The container assembly for sampling a sample with a pipette of the present invention can prevent cell degeneration, prevent cell loss during transportation and storage, and provide a uniform distribution of cell circles in a membrane filter Increase inspection efficiency and ensure accurate inspection.

Referring to FIG. 3, which illustrates a sample container assembly according to an embodiment of the present invention, it comprises a container 100 in which a solution solution and collected cells are accommodated, a connector (not shown) And a filter fixing body 60 for separating the solution solution transferred through the connecting body 50 from the cells.

A cap member 120 detachably coupled to the container body 110 to open and close the opening and having a through hole 121 at the center thereof; And a flexible elastic plate 140 coupled to the through hole 121.

A filter member 130 for filtering the suspended solids in the solution solution is coupled to the cap member 120.

The resilient plate 140 is made of a material such as silicon and has a slender portion 140a thinner than the circumference at the center of the resilient plate 140 to form an entry groove 141 into which the tip of the melter 54 enters.

The elastic plate 140 is fixed to the cap member 120 by a paper sticker 150 having an adhesive on one side thereof. The sticker 150 prevents contamination of the elastic plate 140 and removes the sticker 150 at the time of use.

The connection unit 50 includes a bottom plate 53 formed by protruding upward at a central portion of a dome 54 penetrating the elastic plate 140 and entering the interior of the container 100, An upper engaging portion 51 extending upward from an edge of the bottom plate 53 and detachably coupled to the cap member 120 and a lower engaging portion 52 extending downward from an edge of the bottom plate 53 do.

The connector 50 allows the solution solution and the cells in the container 100 to be guided toward the lower engaging part 52 through the melting engine 54.

A guide 55 is formed on the lower surface of the bottom plate 53 to guide the upper guide member 55 to guide the cells guided to the melter 54 to the center of the membrane filter 63.

The filter fixing body 60 is cylindrical and has an intermediate plate 61 having a suction hole 61a formed at an intermediate portion thereof and an upper end detachably coupled to the lower coupling portion 52, And a membrane filter 63 attached to an upper end of the body 65. The vacuum suction unit 400 is connected to the vacuum suction unit 400. [

A cushion body 64 for passing the solution solution and supporting the membrane filter 63 is provided between the membrane filter 63 and the intermediate plate 61.

On the other hand, a packing 125 of silicone or rubber is attached to the outer circumferential surface of the cap member 120 so that airtightness is maintained when the cap member 120 and the upper engaging portion 51 are coupled, 400 can be precisely controlled.

A method of using the sample container assembly having the above-described configuration is as follows.

First, the solution solution and the cells are received in the container body 110 and the cap member 120 is coupled. Then, as shown in FIG. 3, the container 100 is turned upside down so that the cap member 120 is positioned at the bottom.

The solution solution and the cells C are gathered at the lower part of the cap member 120 while the suspended matters of large particles are filtered through the filter member 130.

4, the connector 50 and the filter fixing body 60 are combined and the vacuum suction device 400 is attached to the coupling portion 62 of the filter fixing body 60. Then, The densifying tube 54 is moved into the container 100 in correspondence with the inlet groove 141 of the elastic plate 140. In this state,

At this time, the thin plate portion 140a of the elastic plate 140 is pierced so that the dental bridge 54 can enter and the upper engaging portion 51 is slidably coupled to the cap member 120 and stably guided. At this time, the gap between the cap member 120 and the upper engaging portion 51 is maintained by the packing 125.

The solution solution and the cells in the container 100 are introduced into the lower engaging portion 52 of the connecting body 50 through the melting orifice 54 by the suction force of the vacuum inhaler 400, The solution solution is discharged through the suction hole 61a of the intermediate plate 61 and the cells are filtered on the upper surface of the membrane filter 63. [

At this time, the cells are concentrated by the guide 55 in the central part of the membrane filter 63 as shown in FIG. 6, so that the cells overlap to form a circle.

In this case, even if the center portion of the cushion body 64 and the membrane filter 63 is bent concave downward as the vacuum suction force is transmitted through the vacuum inhaler 400, the adhesion of the sample cell to the slide 300 Can be improved.

Thereafter, the operator separates the filter fixture 60 and then attaches the sample cell to the surface of the slide 300 by contacting the slide filter 300 with the membrane filter 63, as shown in Fig.

The cushion body 64 provides a cushion when the membrane filter 63 contacts the slide 300 so that the cell C can be easily transferred to and attached to the slide 300, Thereby preventing the cell (C) from being denatured by the contact pressure.

These sample cells are attached to the surface of the slide 300 in the form of a circular cell circle, which has an advantage of improving cell analysis efficiency.

The container assembly of the present invention having the above-described structure prevents the denaturation of the cells because the melting vessel 54 enters the container 100 to take out the cells, unlike the case where the cells are collected by the conventional piston.

In addition, unlike the prior art in which the loss of cells due to the disengagement of the piston occurred, the container assembly of the present invention can prevent the loss of cells during transportation or storage by coupling of the cap member 120.

In addition, the container assembly of the present invention can obtain a uniform distribution of cell circles in the membrane filter 63, thereby enhancing the inspection efficiency and enabling accurate inspection.

50 ... connection body 54 ... fuselage
55 ... Guide 60 ... Filter Fixture
63 ... membrane filter 100 ... container
110 ... container body 120 ... cap member
140 ... elastic plate

Claims (6)

A cap member (110) having a through hole (121) formed at the center thereof by being detachably coupled to the container body (110) and opening and closing the opening And a soft elastic plate (140) coupled to the through hole (121);
A bottom plate 53 having a center portion thereof with a crenellation 54 protruding upward so as to penetrate the elastic plate 140 and enter into the through hole 121 of the container 100; An upper engaging portion 51 extending upward from an edge of the bottom plate 53 and detachably coupled to the cap member 120, a lower engaging portion 52 extending downward from an edge of the bottom plate 53, And a guide (55) located on the inner side of the lower engaging portion (52) and extending downward from a lower surface of the bottom plate (53);
And a vacuum suction unit 400 is coupled to the lower part of the intermediate plate 61. The vacuum suction unit 400 is coupled to the lower part of the intermediate plate 61, And a membrane filter 63 attached to an upper end of the body 65 to be in contact with a lower end of the guide 55. The filter fixing body 60 includes:
The solution solution and the cells in the container 100 are guided to the side of the lower engaging part 52 through the melting orifice 54 by operation of the vacuum inhaler 400, Wherein the pipette comprises a plurality of pipettes. The pipette according to claim 1, wherein the resilient plate (140) comprises a thinned portion (140a) thinner than the periphery at the center thereof, and an inlet groove (141) into which the tip of the melting engine (54) Container assembly for sample collection. A packing 125 is attached to the outer circumferential surface of the cap member 120 or the inner circumferential surface of the upper engaging portion 51 so that airtightness of the cap member 120 when the cap member 120 and the upper engaging portion 51 are engaged, Wherein the pipette is adapted to hold a sample container. delete The container assembly of claim 1, further comprising a filter member (130) inside the cap member (120) for filtering suspended solids in the solution solution. The pipette according to claim 1, further comprising a cushion body (64) for passing the solution solution between the membrane filter (63) and the intermediate plate (61) to support the membrane filter (63) Container assembly for sample collection.
KR1020150175571A 2015-12-10 2015-12-10 A container assembly for picking free medical treatment included pipet KR101825452B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1020150175571A KR101825452B1 (en) 2015-12-10 2015-12-10 A container assembly for picking free medical treatment included pipet
PCT/KR2016/012282 WO2017099357A1 (en) 2015-12-10 2016-10-28 Sample extraction container assembly including pipette

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020150175571A KR101825452B1 (en) 2015-12-10 2015-12-10 A container assembly for picking free medical treatment included pipet

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KR20170069314A KR20170069314A (en) 2017-06-21
KR101825452B1 true KR101825452B1 (en) 2018-02-06

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190135835A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Discharging device of cytology preparing robot
KR20190135834A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Suction and smearing device of cytology preparing robot
KR20190135831A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Gripping and decoupling device of cytology preparing robot

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102248370B1 (en) * 2018-12-11 2021-05-07 (주)바이오트코리아 A module device for cell loading

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101019085B1 (en) * 2010-10-19 2011-03-07 (주)패스텍 Filter container for extracting cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3475355B2 (en) * 2000-08-11 2003-12-08 株式会社クニムネ Urine sample collection and storage equipment
KR100660117B1 (en) * 2004-03-23 2006-12-21 김석곤 Apparatus and method for cell filtering using precipitation method and negative pressure in liquid base cytology
JP2005291940A (en) * 2004-03-31 2005-10-20 Matsushita Electric Ind Co Ltd Microorganism collecting kit
KR101035427B1 (en) * 2010-12-21 2011-05-18 문상호 Filter container for extracting cells
KR101457988B1 (en) * 2012-05-24 2014-11-07 주식회사 바이오씨드 Clinical specimen filter

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101019085B1 (en) * 2010-10-19 2011-03-07 (주)패스텍 Filter container for extracting cells

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190135835A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Discharging device of cytology preparing robot
KR20190135834A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Suction and smearing device of cytology preparing robot
KR20190135831A (en) 2018-05-29 2019-12-09 (주)로봇앤드디자인 Gripping and decoupling device of cytology preparing robot

Also Published As

Publication number Publication date
KR20170069314A (en) 2017-06-21
WO2017099357A1 (en) 2017-06-15

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