CN220201917U - Separation and purification system for centrifugal film filtration - Google Patents
Separation and purification system for centrifugal film filtration Download PDFInfo
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- CN220201917U CN220201917U CN202322729319.5U CN202322729319U CN220201917U CN 220201917 U CN220201917 U CN 220201917U CN 202322729319 U CN202322729319 U CN 202322729319U CN 220201917 U CN220201917 U CN 220201917U
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- 238000001914 filtration Methods 0.000 title claims abstract description 36
- 238000000926 separation method Methods 0.000 title claims abstract description 30
- 238000000746 purification Methods 0.000 title claims abstract description 27
- 210000004027 cell Anatomy 0.000 claims abstract description 31
- 239000012528 membrane Substances 0.000 claims abstract description 26
- 238000005374 membrane filtration Methods 0.000 claims abstract description 16
- 238000007599 discharging Methods 0.000 claims abstract description 4
- 210000001808 exosome Anatomy 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 11
- 239000002245 particle Substances 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 2
- 238000003466 welding Methods 0.000 abstract description 2
- 238000010241 blood sampling Methods 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000003321 amplification Effects 0.000 description 4
- 210000000601 blood cell Anatomy 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 230000004913 activation Effects 0.000 description 2
- 238000001994 activation Methods 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 238000011001 backwashing Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229920006351 engineering plastic Polymers 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to the technical field of medical equipment, in particular to a separation and purification system for centrifugal membrane filtration, which comprises the following scheme: the shell is internally provided with an upper cavity and a lower cavity which are communicated with each other, and the upper cavity and the lower cavity are arranged in a conical shape; a filtering membrane is arranged between the upper cavity and the lower cavity; the upper cavity is provided with a first opening, the lower cavity is provided with a second opening, and the first opening and the second opening are used for feeding or discharging; the conical surface of the upper cavity is provided with a first filter, the conical surface of the lower cavity is provided with a second filter, and the first filter and the second filter are used for guaranteeing the pressure balance in the cavity. The system can be connected with a blood sampling bag, a cell preservation bag and the like in a sterile welding mode to form a closed system, and manual operation links such as opening an interface and the like are reduced, so that the defects that cells are easy to pollute and the production and transportation cost is high are overcome; meanwhile, the filtering membrane can be correspondingly selected according to different particle sizes of cells or exosomes, so that the purpose of separation and purification is achieved.
Description
Technical Field
The utility model relates to the technical field of medical instruments, in particular to a separation and purification system for centrifugal membrane filtration.
Background
The existing cell therapy of immunotherapy mainly comprises the steps of performing immune modification, activation and amplification on immune cells in vitro, wherein the sources are mainly from human blood or tissues, the mixed blood cells are required to be separated and purified in vitro, and then the immune modification, activation and amplification are performed, and the mixed blood cells are returned to the body of a patient for disease treatment.
At present, a density gradient centrifugation method and a filtration method are mainly adopted for separating and purifying cells, wherein the centrifugation method mainly adopts centrifugation and layering through different densities of the cells, and the filtration method mainly adopts separation and purification through different particle sizes of the cells. The centrifugation method needs to add density gradient centrifugate to carry out auxiliary centrifugation separation, wherein the filtration method conventionally adopts vertical filtration, the flowing direction of liquid is consistent with the filtration direction, along with the progress of filtration, the thickness of a filter cake layer or a gel layer formed on the surface of a filter membrane is gradually increased, the flow speed is gradually reduced, the filtration flux and the efficiency are lower, pressure liquid is required to be increased during filtration to pass through the filter membrane, small-particle-size cells can pass through the filter membrane, and large-particle-size cells can be trapped on the filter membrane, and if the target cells are large-particle-size cells, cell collection can be difficult.
Disclosure of Invention
The present utility model is directed to solving at least one of the above problems in the prior art and providing a separation and purification system for centrifugal membrane filtration.
In order to achieve the above object, the present utility model provides a separation and purification system using a novel centrifugal membrane filtration, comprising:
the device comprises a shell, wherein an upper cavity and a lower cavity which are communicated with each other are arranged in the shell, and the upper cavity and the lower cavity are arranged in a conical shape;
a filtering membrane is arranged between the upper cavity and the lower cavity;
a first opening is arranged on the upper cavity, a second opening is arranged on the lower cavity, and the first opening and the second opening are used for feeding or discharging;
the conical surface of the upper cavity is provided with a first filter, the conical surface of the lower cavity is provided with a second filter, and the first filter and the second filter are used for guaranteeing the pressure balance in the cavity.
Preferably, an upper cover and a lower cover are arranged in the shell, and the upper cover and the lower cover are in sealing connection with the shell;
the filtering membrane is arranged between the upper cover and the lower cover, a space surrounded by the upper cover and the filtering membrane is an upper cavity, and a space surrounded by the lower cover and the filtering membrane is a lower cavity.
Preferably, the upper cover and the lower cover are arranged in a conical structure, and the conical tips of the upper cover and the lower cover are arranged back to back.
Preferably, the first opening is disposed at a cone tip of the upper cover, and the second opening is disposed at a cone tip of the lower cover.
Preferably, the first opening is connected with one end of a first pipeline in a sealing way, and the other end of the first pipeline extends to the outer side of the shell;
the second opening is connected with one end of a second pipeline in a sealing way, and the other end of the second pipeline extends to the outer side of the shell.
Preferably, a third opening is formed in the surface of the upper cover, a fourth opening is formed in the surface of the lower cover, the first filter is in sealing connection with the third opening, and the second filter is in sealing connection with the fourth opening.
Preferably, the first filter, the second filter, the first pipeline and the second pipeline are provided with switches.
Preferably, the housing is provided as a cylinder.
Preferably, the first filter and the second filter are air filters of 0.22 μm.
Preferably, the system is used for filtration purification of cells, exosomes.
Based on the above, the utility model has the beneficial effects that:
the utility model adopts a centrifugal filtration mode, and applies pressure in the centrifugal mode when filtering cells, so that cells with small particle size smoothly pass through a filter membrane, meanwhile, the cells are always in liquid when being separated and purified, the pressure among the cells is relatively small, and the loss of the cells is less, so that the amplification culture activity and the activity rate of the cells after separation can be kept; after separation, large-particle-size cells can be collected in a backwashing mode and can be used for the next operation;
the whole system is a closed environment, so that pollution to blood cells caused by personnel and the environment in the operation process is prevented, and the system can be operated in a D-level or C-level environment during industrial application, so that the energy consumption of environment control can be saved.
Drawings
FIG. 1 schematically shows a schematic structure of a separation and purification system for centrifugal membrane filtration according to an embodiment of the present utility model;
FIG. 2 schematically illustrates a top view of a separation and purification system for centrifugal membrane filtration in accordance with one embodiment of the utility model;
description of the drawings: the filter cartridge comprises a housing 10, an upper chamber 101, a first opening 1011, a lower chamber 102, a second opening 1021, a filter membrane 20, a first filter 30, a second filter 40, an upper cover 50, a third opening 501, a lower cover 60, a fourth opening 601, a first pipe 70, a second pipe 80, a switch 90, a first switch 901, a second switch 902, a third switch 903, and a fourth switch 904.
Detailed Description
The present disclosure will now be discussed with reference to exemplary embodiments. It should be understood that the embodiments discussed are merely to enable those of ordinary skill in the art to better understand and thus practice the teachings of the present utility model and do not imply any limitation on the scope of the utility model.
As used herein, the term "comprising" and variants thereof are to be interpreted as meaning "including but not limited to" open-ended terms. The term "based on" is to be interpreted as "based at least in part on". The terms "one embodiment" and "an embodiment" are to be interpreted as "at least one embodiment.
Fig. 1 schematically shows a schematic structural view of a separation and purification system for centrifugal membrane filtration according to an embodiment of the present utility model, and fig. 2 schematically shows a top view of a separation and purification system for centrifugal membrane filtration according to an embodiment of the present utility model, as shown in fig. 1 and 2, the separation and purification system for centrifugal membrane filtration according to the present utility model includes:
the filter comprises a shell 10, wherein an upper cavity 101 and a lower cavity 102 which are communicated with each other are arranged in the shell 10, the upper cavity 101 and the lower cavity 102 are arranged in a conical shape, and a filter membrane 20 is arranged between the upper cavity 101 and the lower cavity 102;
a first opening 1011 is provided on the upper chamber 101, a second opening 1021 is provided on the lower chamber 102, and the first opening 1011 and the second opening 1021 are used for feeding or discharging;
a first filter 30 is provided on the tapered surface of the upper chamber 101, and a second filter 40 is provided on the tapered surface of the lower chamber 102, the first filter 30 and the second filter 40 being used to ensure stable air pressure in the chamber.
Specifically, as shown in fig. 1, the filtering membrane 20 is made of polycarbonate material for separating cell samples, and the filtering membrane 20 can be selected to have a corresponding size according to the particle size of the separated cells or exosomes, thereby achieving the purpose of separation and purification;
an upper chamber 101 and a lower chamber 102 are respectively arranged at two sides of the filtering membrane 20, the upper chamber 101 is communicated with the lower chamber 102, and a sample in the upper chamber 101 can be filtered by the filtering membrane 20 and then enter the lower chamber 102.
The first filter 30 is arranged on the conical surface of the upper cavity 101, the second filter 40 is arranged on the conical surface of the lower cavity 102, the first filter 40 is staggered with the centers of the upper cavity 101 and the lower cavity 102 respectively, when the sample is added into the upper cavity 101 in use, the first filter 30 on the upper cavity 101 needs to be opened, the second filter 40 on the lower cavity 102 is closed, pressure balance is ensured, sample addition can be ensured, meanwhile, when the sample is discharged from the lower cavity 102, the first filter 30 on the upper cavity 101 still needs to be opened, the second filter 40 on the lower cavity 102 is closed, and when the sample is discharged, air enters the cavity from the first filter 30 at the upper cavity 101 for pressure balance, so that the sample in the upper cavity 101 is pressurized, and the sample filtering speed is accelerated.
Further, an upper cover 50 and a lower cover 60 are provided in the housing 10, and the upper cover 50 and the lower cover 60 are hermetically connected with the housing 10;
the filtering membrane 20 is arranged between the upper cover 50 and the lower cover 60, the space enclosed between the upper cover 50 and the filtering membrane 20 is an upper cavity 101, and the space enclosed between the lower cover 60 and the filtering membrane 20 is a lower cavity 102.
The upper cover 50 and the lower cover 60 have a conical structure, and the conical tips of the upper cover 50 and the lower cover 60 are arranged back to back.
Specifically, the upper cover 50 and the lower cover 60 have the same structure and are conical bodies, and the bottom surfaces of the two are opposite to each other, so that the conical tips face to the two ends of the shell 10 respectively;
the diameters of the bottom surfaces of the upper cover 50 and the lower cover 60 are equal to the diameter of the shell 10, so that the upper cover 50 and the lower cover 60 are in interference abutting joint with the inner wall of the shell 10 to form a closed space.
The upper and lower covers 50 and 60 are made of medical engineering plastics, such as PC, PET, ABS.
Further, the first opening 1011 is provided at the cone tip of the upper cover 50, and the second opening 1021 is provided at the cone tip of the lower cover 60, so that the sample can be conveniently added and collected and transferred after centrifugation.
Further, the first opening 1011 is hermetically connected to one end of the first pipe 70, and the other end of the first pipe 70 extends to the outside of the housing 10;
the second opening 1021 is connected with one end of the second pipeline 80 in a sealing manner, and the other end of the second pipeline 80 extends to the outer side of the shell 10.
Specifically, the first pipeline 70 and the second pipeline 80 are sealed hoses, and are connected with a blood collection bag, a cell preservation bag and the like in a sterile welding mode, so that the system of the utility model forms a completely closed environment, the manual operation environment such as opening an interface is reduced, and cell pollution is prevented.
Further, a third opening 501 is provided on the surface of the upper cover 50, a fourth opening 601 is provided on the surface of the lower cover 60, the first filter 30 is connected with the third opening 501 in a sealing manner, the second filter 40 is connected with the fourth opening 601 in a sealing manner, and air enters the chamber through the third opening 501 or the fourth opening 601 after being filtered by the first filter 30 or the second filter 40.
Further, a switch 90 is provided on the first filter 30, the second filter 40, the first pipe 70 and the second pipe 80, the switch 90 includes a first switch 901 provided on the first filter 30, a second switch 902 provided on the second filter 40, a third switch 903 provided on the first pipe 70 and a fourth switch 904 provided on the second pipe 80;
a user can selectively control the opening and closing of the filter and the pipeline at a certain place through the switch 90, so that the use is convenient for the user.
Further, the housing 10 is provided as a cylinder, but may be provided in other shapes.
Further, the first filter 30 and the second filter 40 are air filters of 0.22 μm, and can filter air to ensure stable indoor air pressure.
Further, the use flow of the system is as follows: before adding the sample, the second filter 40 on the lower chamber 102 and the second switch 902 and the fourth switch 904 on the second pipeline 80 are closed, the first filter 30 on the upper chamber 101 and the first switch 901 and the third switch 903 on the first pipeline 70 are opened, the sample is added into the upper chamber 101 from the opening on the first pipeline 70, at this time, the first filter 30 in the upper chamber 101 and the first switch 901 and the third switch 903 on the first pipeline 70 are closed, the system is put into a centrifuge, and the sample is centrifugally separated by the centrifuge;
after centrifugal separation, the first switch 901 on the first filter 30 in the upper cavity 101 and the fourth switch 904 on the second pipeline 80 of the lower cavity 102 are opened, so that the sample is filtered by the filter membrane 20 and discharged from the second pipeline 80 on the lower cavity 102, at this time, in order to ensure the air pressure in the cavity to be stable, air is continuously introduced from the first filter 30 on the upper cavity 101 along with the discharge of the sample from the lower cavity 102, and the sample in the upper cavity 101 is pressurized, thereby accelerating the sample filtering speed and improving the filtering effect;
when the filtered samples have been transferred from the lower chamber 102, the fourth switch 904 on the second line 80 of the lower chamber 102 is closed, the third switch 903 on the first line 70 of the upper chamber 101 is opened, the diluent can be added from the first line 70 to the upper chamber 101, and mixed with the unfiltered sample, after which all the switches 90 are closed, and the system is inverted, the lower chamber 102 is positioned above the upper chamber 101, the system is again placed in the centrifuge for centrifugation, after centrifugation is completed, the system is still in an inverted state, the third switch 903 on the first line 70 of the upper chamber 101 and the second switch 902 of the second filter 40 on the lower chamber 102 are opened, at this time, the unfiltered sample mixed with the diluent is transferred from the first line 70 at the upper chamber 101, and the second filter 40 in the lower chamber 102 is also used for air pressure balancing, and can also pressurize the diluent and accelerate the diluent discharge.
In summary, the centrifugal membrane filtration separation and purification system of the utility model adopts a centrifugal filtration mode, and applies pressure in a centrifugal mode during cell filtration to enable small-particle-size cells to smoothly pass through the filter membrane 20, meanwhile, the pressure among cells is relatively smaller when the cells are separated and purified, and the loss of the cells is less, so that the amplification culture activity and the activity rate of the separated cells can be maintained; after separation, large-particle-size cells can be collected by back flushing and can be used for the next operation.
The whole system is a closed environment, so that pollution to blood cells caused by personnel and the environment in the operation process is prevented, and the system can be operated in a D-level or C-level environment during industrial application, so that the energy consumption of environment control can be saved.
The foregoing description is only of the preferred embodiments of the present application and is presented as a description of the principles of the technology being utilized. It will be appreciated by persons skilled in the art that the scope of the utility model referred to in this application is not limited to the specific combinations of features described above, but it is intended to cover other embodiments in which any combination of features described above or equivalents thereof is possible without departing from the spirit of the utility model. Such as the above-described features and technical features having similar functions (but not limited to) disclosed in the present application are replaced with each other.
It should be understood that, the sequence numbers of the steps in the summary and the embodiments of the present application do not necessarily indicate the order of execution, and the order of execution of the processes should be determined by the functions and internal logic of the steps, and should not be construed as limiting the implementation process of the embodiments of the present application.
Claims (10)
1. A separation and purification system for centrifugal membrane filtration, comprising:
the device comprises a shell, wherein an upper cavity and a lower cavity which are communicated with each other are arranged in the shell, and the upper cavity and the lower cavity are arranged in a conical shape;
a filtering membrane is arranged between the upper cavity and the lower cavity;
a first opening is arranged on the upper cavity, a second opening is arranged on the lower cavity, and the first opening and the second opening are used for feeding or discharging;
the conical surface of the upper cavity is provided with a first filter, the conical surface of the lower cavity is provided with a second filter, and the first filter and the second filter are used for guaranteeing the pressure balance in the cavity.
2. The separation and purification system for centrifugal membrane filtration according to claim 1, wherein an upper cover and a lower cover are provided in the housing, and the upper cover and the lower cover are hermetically connected with the housing;
the filtering membrane is arranged between the upper cover and the lower cover, a space surrounded by the upper cover and the filtering membrane is an upper cavity, and a space surrounded by the lower cover and the filtering membrane is a lower cavity.
3. The separation and purification system for centrifugal membrane filtration according to claim 2, wherein the upper cover and the lower cover are provided in a tapered structure, and the tapered tips of the upper cover and the lower cover are provided to face away from each other.
4. A centrifugal membrane filtration separation and purification system according to claim 3, wherein the first opening is provided at the cone tip of the upper cover and the second opening is provided at the cone tip of the lower cover.
5. The separation and purification system for centrifugal membrane filtration according to claim 1, wherein the first opening is hermetically connected to one end of a first pipe, and the other end of the first pipe extends to the outside of the housing;
the second opening is connected with one end of a second pipeline in a sealing way, and the other end of the second pipeline extends to the outer side of the shell.
6. A centrifugal membrane filtration separation and purification system according to claim 2, wherein a third opening is provided on the surface of the upper cover, a fourth opening is provided on the surface of the lower cover, the first filter is in sealing connection with the third opening, and the second filter is in sealing connection with the fourth opening.
7. The separation and purification system of claim 5, wherein the first filter, the second filter, the first pipeline and the second pipeline are provided with switches.
8. The separation and purification system of claim 1, wherein the housing is configured as a cylinder.
9. The separation and purification system of claim 1, wherein the first filter and the second filter are 0.22 μm air filters.
10. The separation and purification system of claim 1, wherein the system is used for filtration and purification of cells and exosomes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322729319.5U CN220201917U (en) | 2023-10-11 | 2023-10-11 | Separation and purification system for centrifugal film filtration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322729319.5U CN220201917U (en) | 2023-10-11 | 2023-10-11 | Separation and purification system for centrifugal film filtration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220201917U true CN220201917U (en) | 2023-12-19 |
Family
ID=89151972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322729319.5U Active CN220201917U (en) | 2023-10-11 | 2023-10-11 | Separation and purification system for centrifugal film filtration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220201917U (en) |
-
2023
- 2023-10-11 CN CN202322729319.5U patent/CN220201917U/en active Active
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