CN219689669U - Cell centrifugation container, cell separation device and cell separation system - Google Patents
Cell centrifugation container, cell separation device and cell separation system Download PDFInfo
- Publication number
- CN219689669U CN219689669U CN202320121708.7U CN202320121708U CN219689669U CN 219689669 U CN219689669 U CN 219689669U CN 202320121708 U CN202320121708 U CN 202320121708U CN 219689669 U CN219689669 U CN 219689669U
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- cell
- separation
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- centrifugation container
- cup
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- 238000000926 separation method Methods 0.000 title claims abstract description 104
- 238000005119 centrifugation Methods 0.000 title claims abstract description 34
- 230000007246 mechanism Effects 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 24
- 238000007789 sealing Methods 0.000 claims description 4
- 230000005779 cell damage Effects 0.000 abstract description 2
- 208000037887 cell injury Diseases 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 104
- 239000003153 chemical reaction reagent Substances 0.000 description 24
- 239000006228 supernatant Substances 0.000 description 15
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000002659 cell therapy Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004443 dendritic cell Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model discloses a cell centrifugation container which comprises a body, a separation cavity arranged in the body and a conveying pipeline extending into the separation cavity, wherein the separation cavity is conical, and a collection cavity for containing cells to be collected is arranged at the conical tip part of the separation cavity. The cell separation device comprises a rotating frame and the cell centrifugation container, wherein the body is rotatably arranged on the rotating frame. The cell separation system comprises a separation chamber with an opening at the top, the cell separation device is arranged in the separation chamber, a rotary driving mechanism for driving a rotary frame of the cell separation device to rotate is arranged on the separation chamber, and a cover plate is arranged at the opening of the separation chamber. The cell centrifugation container, the cell separation device and the cell separation system have the advantages of reducing cell damage, improving the yield of collected cells, improving the concentration volume and the like.
Description
Technical Field
The utility model relates to the technical field of food and medicine packaging mechanical equipment, in particular to a cell centrifugation container, a cell separation device and a cell separation system.
Background
PBMC is peripheral blood mononuclear cells, human peripheral blood contains lymphocytes, monocytes, dendritic cells and other small amount of cells, a large amount of immune cells can be obtained for cell therapy through separation, screening and culture of PBMC, the existing PBMC separation mode mainly comprises a mode of driving a centrifugal container to rotate for separation, the inner cavity at the bottom of the centrifugal container is conical space, and target cells in a sample settle towards the conical bottom under the action of centrifugal force. However, the yield of the target cells collected by the centrifugation container is low, the concentration volume is small, and in some special centrifugation processes, for example, before the cells are centrifuged, reagents with the density greater than that of the collected cells and without damaging the cells are added, so that the cells are positioned on the reagents during centrifugation, the damage caused by direct contact of the cells with the centrifugation volume is avoided, while the conventional centrifugation container adopts a conical structure (shown in fig. 1), the edges of the cell layer are still in direct contact with the centrifugation container, and the reagent layer is arranged on an inclined plane, so that the thickness of the reagent layer at each position is inconsistent, and the protection effect given to the cells is inconsistent.
In addition, when the centrifugal container at the bottom of the cone discharges the supernatant, the inclined plane can generate radial force on the liquid, so that the layering part of the cells and the supernatant is easier to generate a dispersion phenomenon, namely, the cells and the supernatant are easy to mix, the cells are discharged when the supernatant is discharged, and the yield of the cells is reduced.
Disclosure of Invention
The utility model aims to solve the technical problem of overcoming the defects of the prior art and providing a cell centrifugation container, a cell separation device and a cell separation system which can reduce cell damage and improve the yield and concentration volume of collected cells.
In order to solve the technical problems, the utility model adopts the following technical scheme:
the utility model provides a cell centrifugation container, includes the body, locates the separation chamber in the body and extends to the pipeline in the separation chamber, the separation chamber is the toper, the conical tip portion in separation chamber is equipped with and is used for holding the collection chamber of waiting to collect the cell.
As a further improvement of the above technical scheme:
the collecting cavity is cylindrical.
The conveying pipeline comprises a first pipe body extending to the collecting cavity and a second pipe body extending to the middle part of the separating cavity.
The body comprises a centrifugal cup and a cup cover, the separation cavity is formed in the centrifugal cup, and the cup cover is provided with an opening of the centrifugal cup.
The cup cover is provided with a ventilation membrane, the first pipe body penetrates through the middle of the cup cover, and the second pipe body penetrates through the cup cover and is located on one side of the first pipe body.
A sealing gasket is arranged between the centrifugal cup and the cup cover.
The cell separation device comprises a rotating frame and the cell centrifugation container, wherein the body is rotatably arranged on the rotating frame.
As a further improvement of the above technical scheme:
the rotary frame is provided with a swinging sleeve capable of vertically rotating, and the body is sleeved in the swinging sleeve.
The cell separation system comprises a separation chamber with an opening at the top, the cell separation device is arranged in the separation chamber, a rotary driving mechanism for driving a rotary frame of the cell separation device to rotate is arranged on the separation chamber, and a cover plate is arranged at the opening of the separation chamber.
As a further improvement of the above technical scheme:
the cell separation system further comprises a liquid supply device, a control module and a hanging rack for hanging the liquid supply device are arranged on the separation chamber, the liquid supply device and the rotary driving mechanism are connected with the control module, and the separation cavity is connected with the liquid supply device through a conveying pipeline.
Compared with the prior art, the utility model has the advantages that:
the centrifugal separation process of the cell centrifugation container comprises the following steps: firstly, adding a reagent with the density larger than the density of collected cells and without damaging cells and a cell solution with the collected cells into a separation cavity of a centrifugal container through a conveying pipeline, so that the sum of the total amount of the reagent and the total amount of the cells to be collected is smaller than or equal to the volume of the collection cavity, and forming a reagent layer below the cell solution by the reagent; secondly, the centrifugal container is in a centrifugal state that the cone tip part of the separation cavity faces outwards under the action of centrifugal force, cell liquid is separated into a supernatant layer and a collecting cell layer under the action of centrifugal force, the collecting cell layer is positioned between the supernatant layer and the reagent layer, and the collecting cell layer and the reagent layer are both positioned in the collecting cavity. Therefore, when the centrifugal container is in centrifugal motion, collected cells and reagents in the separation cavity can be both in the collection cavity, and the reagents are positioned between the collected cells and the bottom wall of the collection cavity, so that the collected cells are prevented from being directly extruded on the inner wall of the separation cavity in the centrifugal separation process, the physical damage to the collected cells in the cell centrifugation process is greatly reduced, and the yield of the collected cells is high and the concentration volume is large.
Drawings
FIG. 1 is a schematic cross-sectional view of a conventional centrifuge vessel
FIG. 2 is a schematic perspective view of a cell centrifuge vessel according to the present utility model.
FIG. 3 is a schematic diagram showing the structure of a cell centrifuge vessel according to the present utility model in a main sectional view.
FIG. 4 is a schematic perspective view of a cell separation apparatus according to the present utility model.
FIG. 5 is a schematic perspective view of a cell separation system according to the present utility model.
FIG. 6 is an open state diagram of the cell separation system of the present utility model.
Fig. 7 is an enlarged view at a in fig. 6.
The reference numerals in the drawings denote:
1. a swinging sleeve; 2. a body; 21. a centrifugal cup; 22. a cup cover; 221. a breathable film; 3. a separation chamber; 31. a collection chamber; 4. a first tube body; 5. a second tube body; 6. a sealing gasket; 7. a rotating frame; 8. a separation chamber; 81. a cover plate; 82. a hanging rack; 9. and a control module.
Detailed Description
The utility model will be described in further detail with reference to the drawings and the specific examples.
As used in this disclosure and in the claims, the terms "a," "an," "the," and/or "the" are not specific to a singular, but may include a plurality, unless the context clearly dictates otherwise. The terms "first," "second," and the like, as used in this disclosure, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. Likewise, the word "comprising" or "comprises", and the like, means that elements or items preceding the word are included in the element or item listed after the word and equivalents thereof, but does not exclude other elements or items. The terms "connected" or "connected," and the like, are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.
Embodiment one:
fig. 1 to 3 show a cell centrifugation container according to the present utility model, the cell centrifugation container of the present embodiment comprises a body 2, a separation chamber 3 provided in the body 2, and a transfer pipe extending into the separation chamber 3, the separation chamber 3 is tapered, and a cone tip of the separation chamber 3 is provided with a collection chamber 31 for accommodating cells to be collected.
The centrifugal separation process of the cell centrifugal container comprises the following steps: firstly, adding a reagent with a density higher than the density of collected cells and without damaging cells and a cell liquid with the collected cells into a separation cavity 3 of a centrifugal container through a conveying pipeline, so that the sum of the total quantity of the reagent and the total quantity of the cells to be collected is smaller than or equal to the volume of a collection cavity 31, and forming a reagent layer below the cell liquid by the reagent; next, the centrifugal container is in a centrifugal state in which the cone tip of the separation chamber 3 faces outward by centrifugal force, the cell liquid is separated into a supernatant layer and a collected cell layer by centrifugal force, the collected cell layer is located between the supernatant layer and the reagent layer, and the collected cell layer and the reagent layer are both located in the collection chamber 31. Therefore, when the centrifugal container is in centrifugal motion, collected cells and reagents in the separation cavity 3 can be both in the collection cavity 31, and the reagents are positioned between the collected cells and the bottom wall of the collection cavity 31, so that the collected cells are prevented from being directly extruded on the inner wall of the separation cavity 3 in the centrifugal separation process, the physical damage to the collected cells in the cell centrifugation process is greatly reduced, and the yield of the collected cells is high and the concentration volume is large.
In this embodiment, the collection chamber 31 is cylindrical. Thus, during centrifugation, the reagent chambers are located in the collection chamber 31 at uniform thickness at each location, giving uniform protection to each location of the layer of collected cells. And collect cell and reagent all are in collecting chamber 31, and when discharging the supernatant, the radial force that separation chamber 3 cone produced to the supernatant is difficult to produce great influence to collecting cell in the collecting chamber 31, has weakened the dispersion phenomenon that collecting cell and supernatant produced in the layering department, makes cell and supernatant be difficult to mix to make in the supernatant can not mix a lot of cells together discharge, further improved the yield of cell.
In this embodiment, as shown in fig. 3, the transfer duct includes a first tube 4 extending to the collection chamber 31 and a second tube 5 extending to the middle of the separation chamber 3. The supernatant is discharged through the second pipe body 5; cells are withdrawn through the first tube 4.
In this embodiment, the body 2 includes a centrifugal cup 21 and a cup cover 22, the separation chamber 3 is formed in the centrifugal cup 21, and the cup cover 22 covers an opening of the centrifugal cup 21. The cup cover 22 is provided with a ventilated membrane 221, the first pipe body 4 is penetrated in the middle of the cup cover 22, and the second pipe body 5 is penetrated in the cup cover 22 and is positioned at one side of the first pipe body 4. A sealing gasket 6 is arranged between the centrifugal cup 21 and the cup cover 22.
Through the closed centrifugal container structural design, and the height difference and radial deviation of the first pipe body 4 and the second pipe body 5, the automatic treatment of loading reagents with different components and discharging supernatant can be realized by matching with a closed pipeline system, the whole process realizes full tightness, and the pollution risk in the sample treatment process is greatly reduced. The centrifugal container can be suitable for the functions of density gradient separation of cells, washing of cells, concentration of preparations and the like.
The collection chamber 311 is coaxial with the separation chamber 3. The collecting cavity 311 is far smaller than the separating cavity 3, so that liquid mixing can be effectively avoided during liquid adding, and the concentration of cells can be effectively improved during collecting.
Embodiment two:
fig. 4 shows an embodiment of the cell separation apparatus of the present utility model, which includes a spin stand 7 and the cell centrifugation container of the first embodiment, and the body 2 is rotatably provided on the spin stand 7. The rotating frame 7 rotates to drive the body 2 to rotate for centrifugal movement. The cell separation device comprises all technical characteristics of the cell centrifugation container, and has all advantages of the cell centrifugation container.
Two or more cell centrifugation containers may be mounted on the spin stand 7, and each cell centrifugation container is arranged at intervals around the rotation center of the spin stand 7. As shown in fig. 4, two cell centrifugation containers are symmetrically installed at both ends of the spin frame 7.
In this embodiment, the rotating frame 7 is provided with a swinging sleeve 1 capable of vertically rotating, and the body 2 is sleeved in the swinging sleeve 1. The center axis of the separation cavity 3 is vertical to the swing axis of the swing sleeve 1, and when the swing sleeve is particularly used, the swing axis of the swing sleeve 1 is horizontally arranged.
Embodiment III:
fig. 5 to 7 show an embodiment of the cell separation system of the present utility model, which comprises a separation chamber 8 with an opening at the top, the cell separation device of the second embodiment is disposed in the separation chamber 8, a rotation driving mechanism for driving the rotation frame 7 of the cell separation device to rotate is disposed on the separation chamber 8, and a cover plate 81 is disposed at the opening of the separation chamber 8. The centrifugal movement of the cell separation apparatus is performed in the separation chamber 8, so that the influence of noise on the external environment can be reduced, and no substances are thrown out. The cell separation system comprises all technical characteristics of the cell separation device and has all advantages of the cell separation device.
In this embodiment, the cell separation system further includes a liquid supply device, the separation chamber 8 is provided with a control module 9 and a hanging rack 82 for hanging the liquid supply device, the liquid supply device and the rotation driving mechanism are both connected with the control module 9, and the separation chamber 3 is connected with the liquid supply device through a conveying pipeline. The liquid supply device supplies cell liquid, buffer liquid, reagent, cleaning liquid and the like into the separation chamber 3 through a conveying pipeline. The separation chamber 3 can also be connected to a cell collection device for cell collection via a transfer line. The control module 9 is used for controlling the operation of the liquid supply device and the rotary driving mechanism.
While the utility model has been described in terms of preferred embodiments, it is not intended to be limiting. Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art, or equivalent embodiments with equivalent variations can be made, without departing from the scope of the utility model. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present utility model shall fall within the scope of the technical solution of the present utility model.
Claims (10)
1. A cell centrifugation container, characterized in that: the device comprises a body (2), a separation cavity (3) arranged in the body (2) and a conveying pipeline extending into the separation cavity (3), wherein the separation cavity (3) is conical, and a collection cavity (31) for containing cells to be collected is formed in the conical tip of the separation cavity (3).
2. The cell centrifugation container according to claim 1, wherein: the collecting cavity (31) is cylindrical.
3. The cell centrifugation container according to claim 1, wherein: the conveying pipeline comprises a first pipe body (4) extending to the collecting cavity (31) and a second pipe body (5) extending to the middle part of the separating cavity (3).
4. A cell centrifugation container according to claim 3, wherein: the body (2) comprises a centrifugal cup (21) and a cup cover (22), the separation cavity (3) is formed in the centrifugal cup (21), and the cup cover (22) covers an opening of the centrifugal cup (21).
5. The cell centrifugation container according to claim 4, wherein: the novel cup is characterized in that a breathable film (221) is arranged on the cup cover (22), the first pipe body (4) penetrates through the middle of the cup cover (22), and the second pipe body (5) penetrates through the cup cover (22) and is located on one side of the first pipe body (4).
6. The cell centrifugation container according to claim 4, wherein: a sealing gasket (6) is arranged between the centrifugal cup (21) and the cup cover (22).
7. A cell separation device, characterized in that: comprising a rotating frame (7) and a cell centrifugation container according to any one of claims 1 to 6, the body (2) being rotatably arranged on the rotating frame (7).
8. The cell separation apparatus according to claim 7, wherein: the swinging sleeve (1) capable of vertically rotating is arranged on the rotating frame (7), and the body (2) is sleeved in the swinging sleeve (1).
9. A cell separation system, characterized in that: the cell separation device of claim 7 or 8 is arranged in the separation chamber (8), a rotary driving mechanism for driving a rotary frame (7) of the cell separation device to rotate is arranged on the separation chamber (8), and a cover plate (81) is arranged at the opening of the separation chamber (8).
10. The cell separation system of claim 9, wherein: the cell separation system further comprises a liquid supply device, a control module (9) and a hanging rack (82) for hanging the liquid supply device are arranged on the separation chamber (8), the liquid supply device and the rotary driving mechanism are connected with the control module (9), and the separation cavity (3) is connected with the liquid supply device through a conveying pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320121708.7U CN219689669U (en) | 2023-01-16 | 2023-01-16 | Cell centrifugation container, cell separation device and cell separation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202320121708.7U CN219689669U (en) | 2023-01-16 | 2023-01-16 | Cell centrifugation container, cell separation device and cell separation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219689669U true CN219689669U (en) | 2023-09-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202320121708.7U Active CN219689669U (en) | 2023-01-16 | 2023-01-16 | Cell centrifugation container, cell separation device and cell separation system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219689669U (en) |
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2023
- 2023-01-16 CN CN202320121708.7U patent/CN219689669U/en active Active
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| GR01 | Patent grant | ||
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Effective date of registration: 20240820 Address after: 2909 Tianjiao Fudi Comprehensive Building, No. 99 Dujuan Road, Guanshaling Street, Yuelu District, Changsha City, Hunan Province, China 410023 Patentee after: Changsha Chusi Weikang Intelligent Technology Co.,Ltd. Country or region after: China Address before: No. 197, Section 4, Jinzhou Avenue, Ningxiang Economic Development Zone, Weifeng Community, Ningxiang City Suburban Street, Changsha City, Hunan Province 410600 Patentee before: Chutian Siweikang Gene Technology (Changsha) Co.,Ltd. Country or region before: China |