CN110064230A - A kind of platelet rich plasma acquisition separation vessel - Google Patents

A kind of platelet rich plasma acquisition separation vessel Download PDF

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Publication number
CN110064230A
CN110064230A CN201910468508.7A CN201910468508A CN110064230A CN 110064230 A CN110064230 A CN 110064230A CN 201910468508 A CN201910468508 A CN 201910468508A CN 110064230 A CN110064230 A CN 110064230A
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Prior art keywords
separating pipe
test tube
rubber plug
lumen
platelet
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CN201910468508.7A
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Chinese (zh)
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朱德新
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Individual
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Measuring for diagnostic purposes; Identification of persons
    • A61B5/15Devices for taking samples of blood
    • A61B5/153Devices specially adapted for taking samples of venous or arterial blood, e.g. with syringes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D17/00Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02Separation of non-miscible liquids
    • B01D17/0217Separation of non-miscible liquids by centrifugal force

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Hematology (AREA)
  • Surgery (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Thermal Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to blood collection separation technology fields, specifically a kind of platelet rich plasma acquires separation vessel, including test tube and rubber plug, it is characterized by: being equipped with separating pipe in test tube, separating pipe is lining in test tube interior, for round bottom hollow container, divide three sections of upper, middle and lower, upper end opening, semicircular in shape of going to the bottom sealing, the lumen diameter up and down of separating pipe is identical, middle part lumen is thinner than upper and lower lumen, round bottom below separating pipe is bonded with the bottom of test tube, upper end opening is in the lower part of rubber plug, it is bonded with rubber plug, the outer wall of separating pipe is bonded with the inner wall of test tube, the present invention is compared with the existing technology, the advantage is that: acquisition can be carried out in same container with separating, operating process is more convenient, opportunities for contamination is reduced simultaneously, to obtain the rich platelet of better quality, the design of Density Separation ball and Density Separation gel can be protected Card will not be drawn to red blood cell layer, guarantee the pure of platelet rich plasma in subsequent absorption rich platelet.

Description

A kind of platelet rich plasma acquisition separation vessel
Technical field
The present invention relates to blood collection separation technology fields, and specifically a kind of platelet rich plasma acquisition separation is held Device.
Background technique
The visible component of blood of human body includes red blood cell, leucocyte and blood platelet, and anticoagulated blood specific gravity after being centrifuged is larger Red blood cell and leucocyte be in lower layer, top layer is platelet-poor plasma, and middle layer be platelet rich plasma.1993 Hood proposes platelet rich plasma (PRP) concept first, and finds PRP blood platelet rich in, and number is than number in whole blood High 3 times of mesh or more.Contain a large amount of growth factor in blood platelet, such as platelet derived growth factor (PDGF), conversion growth because Son-β (TGF-β), insulin-like growth factor (IGF), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF) etc.. In the medical domain especially department of stomatology, orthopaedics, beauty treatment fields, the research of platelet rich plasma and using all awfully hot door.
Acquisition is generally divided into multiple devices with separating by the rich platelet separator occurred currently on the market, it is necessary first to Separating pipe is injected with syringe acquisition blood, repeatedly obtains platelet rich plasma after centrifugation, operating process is relatively complicated, Er Qiexu A variety of devices are wanted, while although Special vacuum heparin tube has been used to acquire rich platelet, the extraction of rich platelet is presented and opened Formula operation, the sample of acquisition is easy to be contaminated, and is easy activation blood platelet, eventually lead to unsatisfactory curative effect and because pollution cause it is bad Reaction, influences biological therapy effect.
China discloses a patent of invention: the collection container of mononuclearcell and Platelet-rich plasm, the patent No.: 201410015216.5, which includes outer tube and inner tube, and supporting element is equipped in outer tube, and inner tube is set to outer tube by supporting element Interior, outer tube wall and outer wall of inner tube are in close contact, and are not locked, should be kept inner tube being extracted state easily, although the patent The collection container of current PRP can be overcome to be easy when extracting sample contaminated, blood platelet is easy to be activated and influence biological therapy The defect of effect.But the patent is the utensil of acquisition step, in addition separating step will still choose other utensils.
Summary of the invention
It is an object of the invention to solve the deficiencies in the prior art, provide it is a kind of integrate acquisition and separate, Neng Goubao Demonstrate,prove the technical solution of the acquisition separation vessel of rich platelet quality.
To achieve the goals above, a kind of platelet rich plasma acquisition separation vessel, including test tube 2 and rubber plug 1 are designed, It being characterized in that: being equipped with separating pipe 3 in test tube 2, separating pipe 3 is lining in inside test tube 2, and it is round bottom hollow container, divides three sections of upper, middle and lower, Upper end opening, bottom semicircular in shape sealing, the lumen diameter up and down of separating pipe 3 is identical, and middle part lumen is thinner than upper and lower lumen, separation Round bottom below pipe 3 is bonded with the bottom of test tube 2, and upper end opening is bonded in the lower part of rubber plug 1 with rubber plug 1, outside separating pipe 3 Wall is bonded with the inner wall of test tube 2.
A kind of platelet rich plasma acquires separation vessel, it is characterised in that: 1 top of rubber plug, which is equipped with, to be punctured Hole, lower part are connected with the opening portion of test tube 2, and 3 lower part of rubber plug and 2 opening portion of test tube are interference fitted.
Design a kind of platelet rich plasma acquisition separation vessel, including test tube 2 and rubber plug 1, it is characterised in that: in test tube 2 Equipped with separating pipe 3, separating pipe 3 is lining in inside test tube 2, is round bottom hollow container, is divided three sections of upper, middle and lower, upper end opening, bottom is in Semicircle sealing, the lumen diameter up and down of separating pipe 3 is identical, and middle part lumen is thinner than upper and lower lumen, the round bottom below separating pipe 3 with The bottom of test tube 2 is bonded, and upper end opening is bonded in the lower part of rubber plug 1 with rubber plug 1, the outer wall of separating pipe 3 and the inner wall of test tube 2 It is bonded, Density Separation ball 6 is additionally provided in the separating pipe 3,6 diameter of Density Separation ball is greater than 3 middle part lumen of separating pipe, small In 3 lower part lumen of separating pipe, can be moved freely in 3 lower part lumen of separating pipe, the density of Density Separation ball 6 is between red blood cell Between blood platelet.
A kind of platelet rich plasma acquires separation vessel, it is characterised in that: the Density Separation ball 6 is to have The hollow ball of elasticity.
The present invention compared with the existing technology, the advantage is that:
1. can carry out acquisition with separating in same container, operating process is more convenient, while reducing opportunities for contamination, to obtain Obtain the rich platelet of better quality.
2. the design of Density Separation ball and Density Separation gel can guarantee in subsequent absorption rich platelet, will not It is drawn to red blood cell layer, guarantees the pure of platelet rich plasma.
3. rich platelet can be concentrated on intermediate tubule by primary centrifugation by needs, facilitate collection.
Detailed description of the invention
Fig. 1 is one structural schematic diagram of the present invention program;
Fig. 2 is one usage state diagram of the present invention program;
Fig. 3 is two structural schematic diagram of the present invention program;
Fig. 4 is two usage state diagram of the present invention program;
In figure: the weary blood of 1. rubber plug, 2. test tube, 3. separating pipe, 4. additive, 5. Density Separation gel, 6. Density Separation ball 7. 8. platelet rich plasma of platelet-poor plasma, 9. red blood cell.
Specific embodiment
The technical program is further explained in conjunction with attached drawing.
Embodiment one: referring to Fig. 1 and Fig. 3
The assembly of rich platelet acquisition separation vessel: separating pipe 3 is mounted in the inside of test tube 2 first, the outer wall of separating pipe 3 is close It is fitted in 2 inner wall of test tube, then fills Density Separation gel 5 in 2 bottom of test tube, then additive 4 is added as needed, covers glue Plug 1, there are ventilative gaps for rubber plug 1, as needed using compression rubber plug 1 after vacuum negative pressure device preset vacuum negative pressure.
Rubber plug 1: by having the molding of the halogenated butyl rubber pressing mold of sealing function, puncturing hole, lower part and examination are arranged at the top of rubber plug 1 The opening portion of pipe 2 is connected, and 1 lower part of rubber plug and 2 opening portion of test tube are interference fitted, to guarantee the leakproofness of whole container.
Test tube 2: by glass or have macromolecule transparent material (such as poly terephthalic acid class plastics of air seal function PET, PBT or polycarbonate PC etc.) injection molding, 2 upper opening of test tube is connected with rubber plug 1, and 2 sealed bottom of test tube is in semicircle Shape.
Separating pipe 3: being lining in inside test tube 2, is round bottom hollow container, divides three sections of upper, middle and lower, upper end opening, bottom is in semicircle Shape sealing.The lumen diameter up and down of separating pipe 3 is identical, and middle part lumen is thinner than upper and lower lumen, round bottom and test tube below separating pipe 3 2 bottom fitting, upper end opening are bonded in the lower part of rubber plug 1 with rubber plug 1, and the outer wall of separating pipe 3 is bonded with the inner wall of test tube 2, The optional transparent high molecular material of the material of separating pipe 3, such as polyethylene, polystyrene, polypropylene.
Additive 4: by liquid or solid anti-coagulants, such as sodium citrate, ACD anti-freezing liquid (citric acid, sodium citrate, grape Sugar), heparin, EDTAP dipotassium ethylene diamine tetraacetate etc. mention in liquid form preferably containing the anti-coagulants of sodium citrate when acquiring rich platelet For.
Density Separation gel 5: having a certain viscosity and the active hydrophobic organogels of thixotroping, density between red blood cell and Between blood platelet, preferably 1.050-1.080.
Density Separation ball 6: has the hollow ball of certain elasticity, density is between red blood cell and blood platelet, directly Diameter is greater than the middle part lumen of separating pipe 3, but is less than the diameter of 3 lower part of separating pipe, can be convenient Density Separation ball 6 in lower tube It is moved freely in chamber.Density Separation ball 6 is molded into hollow structure by macromolecular material, in favor of according to hollow size Adjust density.
Embodiment two: referring to fig. 2
Blood collection mixes well later, separates blood through horizontal centrifuge centrifugation (centrifugal force 1700-1900g, 15-20 minute) Slurry, the lowermost part of separating pipe 3 be the maximum red blood cell 9(density of density about 1.093), the lower section in separating pipe, separating pipe 3 Top is about 1.02), in the tubule among separating pipe 3 the smallest platelet-poor plasma 7(density of density is the medium rich blood of density Platelet-poor plasma 8(density about 1.04), Density Separation gel 5(density about 1.05-1.08) connect with middle part in 3 lower part of separating pipe Under the neck at place.The upper surface of Density Separation gel 5 is platelet rich plasma 8, and here is red blood cell 9, since Density Separation is solidifying The barrier action of glue 5 facilitates subsequent platelet rich plasma 8 to acquire.
The internal capacity of container is set: being divided into three sections inside container, upper-lower section is thicker, intermediate elongated, blood collection centrifugation Lower section is mainly red blood cell layer afterwards, according to the specific volume of human erythrocyte, normal male 0.40-0.50, women 0.35-0.45. Desirable mediant 0.40 sets the capacity of red blood cell layer, accordingly radix, can basis when setting the internal capacity of separation vessel Target blood sampling volume sets the volume of each section.
The proportion of additive and blood volume: the main component of additive is anti-coagulants, is provided with is conducive in liquid form The proportion of mixing after blood sampling, additive and blood volume is recommended as 1:20-1:9, and additive volume is unfavorable for blood very little It mixes, will lead to hemodilution too much, be unfavorable for the acquisition of blood platelet.
Density Separation amount of gel and the cooperation for separating pipe diameter: after centrifugation, Density Separation gel should be able to seal completely The section of separating pipe is blocked, highly preferred 5-10 millimeters of Density Separation gel, obstructs red blood cell layer and rich blood completely with guarantee Platelet layer goes out the Density Separation gel weight needed according to the diameter calculation of separating pipe lower section.
Blood collection is separated with platelet rich plasma: using vein blood taking needle, one end connects vein, after puncture, by pipe close Puncture needle is inserted into the rubber plug puncturing hole of platelet rich plasma acquisition separator, and due to the effect of negative pressure in pipe, blood can be inhaled automatically Enter in pipe, according to mixing sample after the scheduled enough blood samplings of negative pressure of vacuum capacity.Using horizontal centrifuge, 1700g centrifugal force from The heart 20 minutes.After centrifugation, 3 lower part of separating pipe is red blood cell 9, and Density Separation gel 5 is in the top of red blood cell 9, and intermediate is thin It is platelet rich plasma 8 in pipe, top is platelet-poor plasma 7.According to needs are used, first with lengthening injection needle Syringe draws the platelet-poor plasma 7 on 3 top of separating pipe, retains the platelet rich plasma 8 in intermediate tubule, then again with matching There is the syringe for lengthening injection needle to draw the platelet rich plasma 8 among tube body.
Embodiment three: referring to fig. 4
Blood collection mixes well later, is centrifuged (centrifugal force 700-1900g, 15-20 minute) separated plasma through horizontal centrifuge, The lowermost part of separating pipe 3 be the maximum red blood cell 9(density of density about 1.093), the lower section in separating pipe 3, in separating pipe 3 Portion is about 1.02), in the tubule among separating pipe 3 the smallest platelet-poor plasma 7(density of density is the medium Fu Xue little of density Plate blood plasma 8(density about 1.04), Density Separation ball 6(density about 1.05-1.08) in 3 lower part of separating pipe and middle part junction Neck, due to the effect of centrifugal force, Density Separation ball 6 is stuck in the upper neck of 3 lower part of separating pipe, Density Separation ball 6 The upper surface of be platelet rich plasma 8, here is red blood cell 9, due to the barrier action of Density Separation ball 6, facilitates subsequent richness Thrombocyte plasma acquisition.

Claims (4)

1. a kind of platelet rich plasma acquires separation vessel, including test tube (2) and rubber plug (1), it is characterised in that: in test tube (2) Equipped with separating pipe (3), it is round bottom hollow container that it is internal that separating pipe (3) is lining in test tube (2), divides three sections of upper, middle and lower, upper end opening, Semicircular in shape of going to the bottom sealing, the lumen diameter up and down of separating pipe (3) is identical, and middle part lumen is thinner than upper and lower lumen, under separating pipe (3) The round bottom in face is bonded with the bottom of test tube (2), and upper end opening is bonded in the lower part of rubber plug (1) with rubber plug (1), separating pipe (3) Outer wall is bonded with the inner wall of test tube (2).
2. a kind of platelet rich plasma as described in claim 1 acquires separation vessel, it is characterised in that: on the rubber plug (1) Portion is equipped with puncturing hole, and lower part is connected with the opening portion of test tube (2), and rubber plug (1) lower part and test tube (2) opening portion are interference fitted.
3. a kind of platelet rich plasma acquires separation vessel, including test tube (2) and rubber plug (1), it is characterised in that: in test tube (2) Equipped with separating pipe (3), it is round bottom hollow container that it is internal that separating pipe (3) is lining in test tube (2), divides three sections of upper, middle and lower, upper end opening, Semicircular in shape of going to the bottom sealing, the lumen diameter up and down of separating pipe (3) is identical, and middle part lumen is thinner than upper and lower lumen, under separating pipe (3) The round bottom in face is bonded with the bottom of test tube, and upper end opening is bonded in the lower part of rubber plug (1) with rubber plug (1), outside separating pipe (3) Wall is bonded with the inner wall of test tube (2), is additionally provided with Density Separation ball (6) in the separating pipe (3), and Density Separation ball (6) is straight Diameter is greater than lumen in the middle part of separating pipe (3), is less than separating pipe (3) lower part lumen, can freely move in the lumen of separating pipe (3) lower part Dynamic, the density of Density Separation ball (6) is between red blood cell and blood platelet.
4. a kind of platelet rich plasma as claimed in claim 3 acquires separation vessel, it is characterised in that: the Density Separation circle Ball (6) is the hollow ball for having elasticity.
CN201910468508.7A 2019-05-31 2019-05-31 A kind of platelet rich plasma acquisition separation vessel Pending CN110064230A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115960826A (en) * 2022-12-07 2023-04-14 上海医涯医疗科技有限公司 Horizontal centrifugal separation method for cells

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101147841A (en) * 2004-03-26 2008-03-26 乔纳桑·欧文 Separator for immiscible liquids
KR20110009651A (en) * 2010-12-31 2011-01-28 주식회사 메디사랑 A blood separating vial, a blood concentrating vial for extracting platelet rich plasma and a method for extracting prp using the same
CN106366426A (en) * 2016-08-30 2017-02-01 成都瑞琦科技实业股份有限公司 Separation gel system for platelet-rich plasma (PRP) extraction and purification and preparation method thereof
CN106582908A (en) * 2016-11-30 2017-04-26 深圳市达科为生物工程有限公司 Platelet-rich plasma rapid separating device and method
CN208087619U (en) * 2018-03-13 2018-11-13 武汉东方华康科技有限公司 A kind of cell collection preservation separating pipe
CN109266531A (en) * 2018-10-08 2019-01-25 陈琼娣 A kind of peripheral blood mononuclear cells separating pipe and preparation method thereof
CN210384938U (en) * 2019-05-31 2020-04-24 朱德新 Platelet-rich plasma collecting and separating container

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101147841A (en) * 2004-03-26 2008-03-26 乔纳桑·欧文 Separator for immiscible liquids
KR20110009651A (en) * 2010-12-31 2011-01-28 주식회사 메디사랑 A blood separating vial, a blood concentrating vial for extracting platelet rich plasma and a method for extracting prp using the same
CN106366426A (en) * 2016-08-30 2017-02-01 成都瑞琦科技实业股份有限公司 Separation gel system for platelet-rich plasma (PRP) extraction and purification and preparation method thereof
CN106582908A (en) * 2016-11-30 2017-04-26 深圳市达科为生物工程有限公司 Platelet-rich plasma rapid separating device and method
CN208087619U (en) * 2018-03-13 2018-11-13 武汉东方华康科技有限公司 A kind of cell collection preservation separating pipe
CN109266531A (en) * 2018-10-08 2019-01-25 陈琼娣 A kind of peripheral blood mononuclear cells separating pipe and preparation method thereof
CN210384938U (en) * 2019-05-31 2020-04-24 朱德新 Platelet-rich plasma collecting and separating container

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115960826A (en) * 2022-12-07 2023-04-14 上海医涯医疗科技有限公司 Horizontal centrifugal separation method for cells
CN115960826B (en) * 2022-12-07 2023-10-17 上海医涯医疗科技有限公司 Cell horizontal centrifugal separation method

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