CN114160226A

CN114160226A - Centrifugal separation tube and manufacturing method and application thereof

Info

Publication number: CN114160226A
Application number: CN202111419176.7A
Authority: CN
Inventors: 潘维超; 武佩; 肖国锋; 胡政芳; 何琴玲; 施晓红
Original assignee: NANJING SHUANGWEI BIOTECHNOLOGY CO Ltd
Current assignee: NANJING SHUANGWEI BIOTECHNOLOGY CO Ltd
Priority date: 2021-11-26
Filing date: 2021-11-26
Publication date: 2022-03-11
Also published as: WO2023093781A1

Abstract

The invention discloses a centrifugal separation tube and a manufacturing method and application thereof. The centrifugal separation tube comprises an outer tube body, a central liquid taking tube and a bottom cover, wherein the outer tube body is divided into an upper bin body, a throat bin and a lower bin body, the upper bin body is provided with an injection port, an air vent and a suction port, the bottom end of the lower bin body is open and is in sealing fit with the bottom cover, and the axial position of the bottom cover is adjustable; the lower end of the central liquid taking pipe is a liquid taking pipe orifice and is positioned in the throat bin, and the upper end of the central liquid taking pipe is communicated with the suction port. The centrifugal separation tube is provided with the central liquid taking tube, so that a middle layer product can be preferentially sucked when the centrifugal separation tube is applied to the centrifugal separation of liquid, and when the middle layer is a required product, the centrifugal separation process can be optimized, and the operation is simplified; the centrifugal blood platelet-rich plasma sucking device is applied to the centrifugal preparation of the blood platelet-rich plasma, can directly suck the blood platelet concentrate in the middle layer and quantitatively suck the blood platelet-poor plasma, and omits the processes of red blood cell separation and intermediate liquid transfer, thereby shortening the operation time and reducing the pollution risk.

Description

Centrifugal separation tube and manufacturing method and application thereof

Technical Field

The invention relates to the technical field of biological medicines and medical instruments, in particular to a centrifugal separation tube and a manufacturing method and application thereof.

Background

Platelet-rich plasma (PRP) is rich in bioactive substances such as growth factors, cytokines and antibacterial peptides, has the effects of promoting cell proliferation, differentiation, matrix synthesis, tissue regeneration and repair, and plays an important role in regenerative medicine. The preparation methods of PRP are more, and at present, there is no unified standard, and the following mature methods are introduced in the literature, "expert consensus on autologous platelet-rich plasma preparation technology" (single Guiqiu, etc.): (1) the method comprises the steps of (1) collecting and preparing PRP by a blood component single collector, (2) collecting and preparing PRP by a blood bag, (3) preparing PRP by a special PRP separation kit, and (4) manually collecting whole blood by a test tube method to prepare PRP.

Wherein, the third method adopts a whole set of preparation consumables which are specially designed for PRP preparation and are equipped with a centrifuge, and the method is characterized in that: PRP is collected in a fully closed or semi-closed state, and the safety of the product is relatively high; the concentration of the blood platelet can be more than or equal to 4 times of the basic concentration of the blood platelet of the patient. Based on the characteristics of blood, the method mostly adopts a secondary gradient centrifugation method to perform separation operation: the first centrifugation can divide the blood into 3 layers, the bottommost part is red blood cells accounting for about 55% of the total volume of the blood, the top part is platelet-poor plasma (PPP) accounting for about 40% of the total volume, mainly plasma components such as fibrinogen, and the middle layer is Platelet Concentrate (PC) accounting for only 5% of the total volume, namely a yellow coating layer or a white membrane layer; after the platelet poor plasma, the platelet concentrate and a small amount of red blood cells are sucked, the second centrifugation is carried out to obtain the platelet poor plasma at the upper layer and the platelet rich plasma at the lower layer. The main defects of the secondary blood centrifugal separation method are that two times of centrifugal operation respectively need one set of centrifuge tube as a preparation consumable, the cost is high, the generated medical wastes are more, the liquid transfer process is more troublesome, and more serious, the pollution risk is increased.

Patent literature (CN 203609925U, closed platelet concentrator) discloses a closed platelet concentrator, which is designed skillfully to complete two times of centrifugation in one set of consumable, but obviously, the structure is complex and the cost of the consumable is high.

Disclosure of Invention

In order to overcome the defects of the closed platelet concentrator, the invention provides a centrifugal separation tube and a manufacturing method and application thereof.

The technical scheme adopted by the invention is as follows: a multiphase centrifugal separation tube comprises an outer tube body, a central liquid taking tube and a bottom cover, wherein the outer tube body is composed of an upper bin body, a throat bin body and a lower bin body which are sequentially communicated, and the inner diameter of the throat bin body is smaller than that of the upper bin body and that of the lower bin body; the top of the upper bin body is closed, an injection port, an air vent and a suction port are arranged, the bottom end of the lower bin body is open and is in sealing fit with the bottom cover, and the axial position of the bottom cover relative to the lower bin body is adjustable; the lower end of the central liquid taking pipe is a liquid taking pipe orifice and is positioned in the throat bin, and the upper end of the central liquid taking pipe is integrally fixed with the upper bin body and is communicated with the suction port.

Preferably: the bottom cover is a screw cover and is connected with the lower bin body through threads, and a bottom sealing ring is arranged between the lower bin body and the bottom cover; the section of the sealing ring is dumbbell-shaped.

Preferably: the throat bin is provided with a window which is aligned with the liquid taking pipe orifice and used for photoelectric detection.

Preferably: the liquid taking pipe orifice is attached to the inner wall of the throat bin.

Preferably: the sprue is equipped with the rubber buffer, the blow vent is equipped with hinders the fungus membrane, suction opening department is equipped with the diaphragm.

Preferably: the injection opening and the vent opening are arranged on the top of the circumferential wall of the upper bin body, and the suction opening is arranged on the top wall of the upper bin body.

Preferably: the upper bin body is also provided with a top spiral cover, the top spiral cover is respectively provided with an injection outer opening and a ventilation outer hole corresponding to the injection opening and the ventilation opening, a rubber outer plug is arranged in the injection outer opening, and a bacterium blocking outer membrane is arranged in the ventilation outer hole; and a second sealing element is arranged between the end wall of the top spiral cover and the outer pipe wall of the suction port, and a third sealing element is arranged between the circumferential wall of the top spiral cover and the outer circumferential wall of the upper bin body.

Preferably: the injection opening and the vent are horizontally arranged on the edge of the top wall of the upper cabin body in the form of luer connectors.

Preferably: the multiphase centrifugal separation tube is used for preparing platelet-rich plasma through one-time centrifugation.

A manufacturing method of a multiphase centrifugal separation tube comprises the following steps:

step 1, integrally molding an outer tube body which is communicated up and down, wherein the outer tube body comprises an upper bin body, a throat bin and a lower bin body;

step 2, ultrasonically welding or gluing the upper end of the central liquid taking pipe on a suction port on the top wall of the upper bin body, and ultrasonically welding or gluing the lower end of the central liquid taking pipe on the inner wall of the throat bin;

step 3, welding or cementing the top wall of the upper bin body and the outer tube body into a whole;

and 4, assembling a rubber plug and a bacterium blocking film on the injection opening and the vent on the upper bin body respectively, and assembling a bottom cover on the lower bin body.

The invention has the following beneficial effects:

1. the centrifugal separation tube can be applied to centrifugal separation of liquid, and is characterized in that the central liquid taking tube is arranged, so that a middle layer product can be preferentially sucked, and when the middle layer is a required product, the centrifugal separation process can be obviously optimized, and the operation is simplified;

2. the invention is applied to the preparation of platelet rich plasma by centrifugation, can directly absorb platelet concentrate in the middle layer and then quantitatively absorb platelet poor plasma, and omits the processes of red blood cell separation and middle liquid transfer, thereby shortening the operation time and reducing the pollution risk.

Drawings

FIG. 1 is a perspective view of the consumable of the centrifuge tube according to the first embodiment of the present invention.

FIG. 2 is a schematic front view of the consumable of the centrifuge tube according to the first embodiment of the present invention.

FIG. 3 is a cross-sectional view of the consumable A-A of the centrifuge tube according to the first embodiment of the present invention.

FIG. 4 is a cross-sectional view of the consumable B-B of the centrifuge tube according to the first embodiment of the present invention.

FIG. 5 is an enlarged view of the consumable part of the centrifuge tube at point C in the first embodiment of the present invention.

FIG. 6 is a perspective view of the consumable of the centrifuge tube in the second embodiment of the present invention.

FIG. 7 is a schematic front view of the consumable of a centrifuge tube according to a second embodiment of the present invention.

FIG. 8 is a cross-sectional view of the consumable D-D of a centrifuge tube according to a second embodiment of the present invention.

FIG. 9 is an enlarged view of consumable E of the centrifuge tube in the second embodiment of the present invention.

FIG. 10 is a perspective view of the consumable in a centrifuge tube according to a third embodiment of the present invention.

An outer tube body 1, an upper chamber body 101, a lower chamber body 102, a throat chamber 103, an injection port 104, a vent 105, a rubber plug 106, a bacteria-blocking film 107, a suction port 108, a diaphragm 109 and a window 110; a central liquid taking pipe 2 and a liquid taking pipe opening 201; a bottom cover 3; a bottom seal ring 4; a top screw cap 5, an injection outer opening 501, a ventilation outer hole 502, a rubber outer plug 503 and a bacterium blocking outer film 504; seal number two 6; seal No. three 7.

Detailed Description

The following is a description of the present invention with reference to the accompanying drawings, using the present invention in the preparation of platelet rich plasma by centrifugation.

Example one

As shown in fig. 1-5, the consumable of the centrifuge tube of the present embodiment has an outer tube body 1, a special central liquid-extracting tube 2 is disposed therein, and the accurate alignment of the liquid-extracting tube opening 201 of the central liquid-extracting tube 2 is ensured to use the interface of the platelet concentrate and the red blood cells as the reference designated position. However, since the ratio of each component in the blood from different sources varies depending on the individual blood source, it is difficult to ensure that the port 201 of the central aspiration tube 2 is at a predetermined position with respect to the interface between the platelet concentrate and the red blood cells after centrifugation of each whole blood. There are two kinds of solutions here, first the center liquid taking pipe 2 is the activity pipe, and the mouth of pipe position is adjustable, second the volume of outer tube body 1 is adjustable, promptly the position of the interface of composition blood is adjustable. Since the first scheme is difficult to implement, the second scheme is adopted in the embodiment.

Specifically, as shown in fig. 1 to 5, the design specification of the outer tube body 1 of the centrifuge tube consumable is generally within the range of 5 to 100mL, usually 10mL, 15mL, 30mL, 40mL and 60mL, and the diameter is 10 to 50 mm. Is divided into an upper bin body 101 and a lower bin body 102, and the upper bin body 101 and the lower bin body 102 are communicated into a whole through a throat bin 103. The built-in central liquid intaking pipe 2 that has of outer tube shaft 1, the lower extreme of central liquid intaking pipe 2 is for getting liquid mouth of pipe 201, is located the throat storehouse 103, and the upper end of central liquid intaking pipe 2 welds or bonds fixedly with the roof of last storehouse body 101 to with suction port 108 intercommunication on the roof. The lower part of the lower bin body 102 is open and is provided with a bottom cover 3 in threaded connection, and a bottom sealing ring 4 is arranged between the bottom cover 3 and the lower bin body 102. The volume of the outer tube body 1, namely the position of the interface of the platelet concentrate and the red blood cells can be adjusted by rotating the screw cap 3.

As shown in FIGS. 1 to 5, an injection port 104 and a vent 105 are formed on the top of the peripheral wall of the upper chamber body 101. The injection port 104 is provided with a rubber plug 106, the rubber plug 106 is fastened through a compression ring, and the compression ring is fixed with the upper bin body 101 into a whole by ultrasonic welding or gluing. The vent 105 is provided with a bacteria-blocking film 107 so that the vent 105 can block bacteria and ventilate. A diaphragm 109 is arranged at the suction port 108 and is formed by injection molding. The rubber plug 106, the diaphragm 109 and the bacteria-blocking film 107 respectively play a role in sealing and bacteria-blocking. The rubber stopper 106 can be pierced by a needle when whole blood is injected, the sealing can be kept during centrifugation, the diaphragm 109 is kept sealed before suction, and the diaphragm is broken once when platelet rich plasma is sucked.

As shown in figures 3-4, the liquid taking pipe opening 201 at the lower end of the central liquid taking pipe 2 is attached to the inner wall of the throat bin 103, and the structure utilizes the cohesion and the adsorbability of liquid, so that the suction effect can be improved, and the platelet concentrate is prevented from remaining in the outer pipe body 1.

As shown in fig. 5, the bottom sealing ring 4 between the bottom cover 3 and the lower chamber body 102 has a dumbbell-shaped cross section and a large sealing area. The sealing structure has two advantages, namely, good sealing performance is provided, leakage and pollution are avoided, a certain friction force is kept, the bottom cover 3 cannot rotate easily, the volume of the outer tube body 1 is stable, and the stability and the reliability of the device are guaranteed.

As shown in fig. 1, the circumferential wall of the bottom cover 3 has a plurality of planes, which facilitates the automatic rotation after the mechanical clamping. Correspondingly, as shown in fig. 1, a window 110 corresponding to the liquid taking nozzle 201 is arranged on the throat bin 103, the photoelectric sensor detects through the window 110, and recognizes red blood cells on the lower layer by using the light transmittance, so as to distinguish the interface between the platelet concentrate and the red blood cells, and further control the mechanical device to drive the bottom cover 3, thereby completing the adjustment of the liquid level, and enabling the interface and the liquid taking nozzle 201 at the lower end of the central liquid taking tube 2 to be at a specific position. The inner diameter of the throat chamber 103 is generally 1/3 of the inner diameters of the upper chamber body 101 and the lower chamber body 102, so that the platelet concentrate can show a higher height for detection and operation.

In addition, the centrifuge tube consumables are manufactured by adopting the process of injection molding and ultrasonic welding/bonding, and the centrifuge tube consumables have the advantages of simple and reliable process. The material is a medical grade transparent material, in particular ABS, and the processing technology is as follows:

step 1, injection molding: an integrally injection-molded outer tube body 1 which is communicated up and down comprises an upper bin body 101, a throat bin 103 and a lower bin body 102.

Before injection molding, the variety, specification and grade of the ABS material are checked, and the ABS material is observed, so that the ABS material is required to be uniform in color and luster and free of foreign matters. And opening the injection molding machine, and raising the temperature to a rated value, wherein the temperature in the zone I is 225 +/-15 ℃, the temperature in the zone II is 220 +/-15 ℃, the temperature in the zone III is 220 +/-15 ℃, the temperature in the zone IV is 215 +/-15 ℃ and the temperature in the zone V is 220 +/-15 ℃. Adjusting the technological parameters of the injection molding machine, and keeping the temperature for 15min after the temperature is reached. Manually injecting a head material, observing the full dissolution of the material, adjusting the injection and pressure maintaining time to be 7 +/-2 s, cooling the material for 13 +/-3 s, manually closing the mold, performing mold locking under the pressure of 5 +/-2 MPa, performing injection molding on 2-3 molds, and continuously and normally producing the injection molding under the pressure of 7 +/-2 MPa after the product is qualified.

And (4) checking the appearance, having no bubble and no flash phenomenon, checking the inner diameter and the press-in diameter of the socket according to the requirements of the check card, and entering the intermediate storage after the detection is qualified.

And 2, ultrasonically welding the upper end of the central liquid taking pipe 2 on a suction port 108 on the top wall of the upper bin body 101, and ultrasonically welding the lower end on the inner wall of the throat bin 103.

And 3, welding or gluing the top wall of the upper bin body 101 and the outer tube body 1 into a whole.

And 4, assembling a rubber stopper 106 and a bacterium blocking film 107 on the injection opening 104 and the vent opening 105 on the upper bin body 101 respectively, and assembling a bottom cover 3 on the lower bin body 102.

The embodiment is applied to a one-time centrifugal preparation method of the platelet-rich plasma, and is used for preparing the platelet-rich plasma with high baseline (concentration ratio of 5-9 times).

The method comprises the following specific steps:

step 1, injecting whole blood mixed with anticoagulant into a centrifuge tube consumable, placing the centrifuge tube consumable on a centrifuge for centrifugal separation, wherein the whole blood is divided into three layers in the centrifuge tube consumable, the upper layer is platelet poor plasma, the middle layer is platelet concentrate, and the lower layer is red blood cells.

Specifically, the consumable of the centrifuge tube in the second embodiment has a nominal volume of 30mL, an inner diameter of 10mm, and a volume of 30mL for whole blood. The anticoagulant is citric sodium powder, and each milliliter of blood is added with 3-5 mg. The centrifuge is commercially available centrifuge equipment, and the centrifugation parameter is 900g for 9 min. In addition, the whole blood sample in the examples is a cryopreserved sample, and therefore the concentration of platelets is low.

And 2, extracting the platelet concentrate in the middle layer, and settling the platelet poor plasma in the upper layer.

And 2.1, detecting by the photoelectric sensor through the window 110, identifying red blood cells on the lower layer by utilizing the light transmittance, distinguishing an interface of the platelet concentrate and the red blood cells, automatically adjusting the vertical position of the interface of the platelet concentrate and the red blood cells in a consumable of the centrifuge tube, and enabling a liquid taking pipe opening of the central liquid taking pipe to be located at a position 1-2 mm above the interface.

In particular, the position of the interface between the platelet concentrate and the red blood cells can be adjusted according to clinical or scientific requirements. In some cases, a certain amount of red blood cells is allowed to be contained in the platelet rich plasma, and the liquid taking nozzle of the central liquid taking tube can be positioned about 1mm below the interface.

And 2.2, a piston type suction device is used for sucking the platelet concentrate through the central liquid taking pipe 2 until a liquid taking pipe opening of the central liquid taking pipe is contacted with the upper layer of the platelet poor plasma, and automatic control is realized by a photoelectric sensor.

Step 2.3. the volume of platelet concentrate drawn V1 is measured by a piston suction device. The piston type suction device is controlled by a stepping motor, the stepping motor rotates for one circle, the suction device sucks 0.1mL, and therefore the volume V1 of the extracted platelet concentrate can be accurately measured through the rotation angle of the stepping motor. Here, V1 was about 1.5 mL.

And 3, continuously extracting part or all of the settled platelet poor plasma to obtain platelet rich plasma.

Step 3.1, determining the volume of the platelet poor plasma needing to be extracted V2, V2= nV1, and n is a proportional relation coefficient of V2 and V1 according to the volume of the extracted platelet concentrate V1.

According to experience, preparing platelet-rich plasma with high-power baseline (concentration ratio of 5-9 times), wherein n is 1.0-3.0 generally; preparing platelet-rich plasma with low-fold baseline (concentration ratio of 2.5-3 times), wherein n is generally 5.0-7.0. N values can also be obtained from multiple experiments for different blood sources. Here, n =1.2 (in the range of 1.0 to 3.0), i.e., V2 was 1.8 mL.

And 3.2, continuously utilizing the suction device to draw 1.8mL of platelet poor plasma, namely directly obtaining about 3.0mL of platelet rich plasma.

The platelet concentration in whole blood before separation was determined to be 31x10⁹The platelet concentration of the obtained platelet-rich plasma was 247x10⁹The concentration ratio of the prepared platelet-rich plasma is 7.97 times.

From the above analysis, the present embodiment has the following advantages:

1. compared with the traditional one-time centrifugation method, the prepared PRP has higher concentration and purity, and each component is more convenient to extract;

2. compared with a secondary centrifugation method, the method is more convenient and faster, the intermediate liquid transfer process of the secondary centrifugation method is omitted, the operation time is shortened, and the pollution risk is reduced;

3. the specially-made centrifuge tube consumables are adopted, so that platelet concentrates are completely sucked firstly, then platelet poor plasma is sucked, and platelet rich plasma is directly obtained, so that scientific research or clinical requirements are better met;

4. the centrifuging tube consumptive material structure is simple relatively, and spare part is few, has reduced the cost of PRP preparation, and in addition, the medical waste that produces is also still less.

Example two

As shown in fig. 6 to 9, the present embodiment is substantially the same as the first embodiment, and the difference is the structure of the consumable material of the centrifuge tube. Specifically, the upper bin body 101 of the outer tube body 1 is further provided with an additional top screw cap 5, the circumferential wall of the top screw cap 5 is provided with an injection outer opening 501 and a ventilation outer hole 502 corresponding to the injection opening 104 and the ventilation opening 105 on the upper bin body 101, similarly, the injection outer opening 501 is provided with a rubber outer plug 503, and the ventilation outer hole 502 is provided with a bacteria-blocking outer membrane 504. In addition, a second sealing element 6 is arranged between the end cover of the top rotary cover 5 and the upper end of the central liquid taking pipe 2, and a third sealing element 7 is arranged between the circumferential wall of the top rotary cover 5 and the outer circumferential wall of the upper bin body 101.

The top spiral cover 5 of this embodiment is connected through the mode of buckle with the last storehouse body 101, and axial fixity, circumference are rotatable, therefore top spiral cover 5 has two kinds of positions: the first position is where the injection port 501 is aligned with the injection port 104 for injection operation, and the second position is where the injection port 501 is offset from the injection port 104 for centrifugation or extraction operation.

The advantage of this embodiment lies in that the centrifuging tube consumptive material has the fungus and protect function of hindering of bilayer, and security and reliability are higher in the operation process.

The material of centrifuging tube consumptive material in the second embodiment adopts PC, and the processing technology of centrifuging tube consumptive material is basically the same with the first embodiment, because raw and other materials are different, therefore the injection moulding process also has the difference. Pre-drying materials before injection molding: placing the PC material particles in a 800-air-volume dehumidifier for dehumidification and drying. A dehumidification part: dehumidification wind temperature 80 + -10 deg.C, regeneration temperature: 180 ± 10 ℃, hopper 1 temperature: 75 +/-10 ℃ and the temperature of the hopper 2 is 120 +/-10 ℃. And opening a mold temperature controller in advance before injection molding to preheat the mold. And (3) starting the injection molding machine, keeping the temperature for more than 10min after the temperature is raised to a preset value, injecting a plurality of molds in a test mode to observe the appearance of the injection molding piece after head materials are discharged in an empty mode, and producing the injection molding piece after the requirements are met. The temperature of the mold temperature controller was set to 60. + -. 20 ℃. The injection molding parameters are as follows: the temperatures from zone I to zone III are respectively 310 +/-15 ℃, 305 +/-15 ℃ and 300 +/-15 ℃; mold locking pressure: 6 +/-2 MPa; the injection pressure is 9 plus or minus 1 Mpa; the injection time is 3 +/-2 s, the dwell time is 2 +/-1 s, and the cooling time is 14 +/-2 s.

EXAMPLE III

As shown in fig. 10, this embodiment is substantially the same as the first embodiment, and the difference is also the structure of the outer tube body 1 of the consumable of the centrifuge tube. Specifically, the injection port 104 and the vent 105 are horizontally arranged on the end cap of the upper cartridge body 101 in the form of a luer connector. The advantage of this embodiment lies in that the equipment is convenient, and convenient and complete sets cooperation is used.

It should be understood that the above-described embodiments of the present invention are merely examples for illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other obvious variations or modifications of the present invention are within the scope of the present invention, which is not limited thereto.

Claims

1. The centrifugal separation tube is characterized by comprising an outer tube body (1), a central liquid taking tube (2) and a bottom cover (3), wherein the outer tube body (1) is divided into an upper chamber body (101), a throat chamber (103) and a lower chamber body (102) which are sequentially communicated, and the inner diameter of the throat chamber (103) is smaller than that of the upper chamber body (101) and that of the lower chamber body (102);

the top of the upper bin body (101) is closed, an injection port (104), an air vent (105) and a suction port (108) are arranged, the bottom end of the lower bin body (102) is open and is in sealing fit with the bottom cover (3), and the axial position of the bottom cover (3) relative to the lower bin body (102) is adjustable;

the lower end of the central liquid taking pipe (2) is a liquid taking pipe orifice (201) which is positioned in the throat bin (103), and the upper end of the central liquid taking pipe is integrally fixed with the upper bin body (101) and is communicated with the suction port (108).

2. The centrifuge tube of claim 1, wherein: the bottom cover (3) is a screw cover and is connected with the lower bin body (102) through threads, and a bottom sealing ring (4) is arranged between the lower bin body (102) and the bottom cover (3); the section of the sealing ring (4) is dumbbell-shaped.

3. The centrifuge tube of claim 1, wherein: the throat bin (103) is provided with a window (110) which is aligned with the liquid taking pipe orifice (201) and used for photoelectric detection.

4. The centrifuge tube of claim 1, wherein: the liquid taking pipe orifice (201) is attached to the inner wall of the throat bin (103).

5. The centrifuge tube of claim 1, wherein: the filling opening (104) is provided with a rubber plug (106), the vent opening (105) is provided with a fungus blocking film (107), and a diaphragm (109) is arranged at the suction opening (108).

6. The centrifuge tube of claim 5, wherein: the injection opening (104) and the vent opening (105) are arranged at the top of the circumferential wall of the upper cabin body (101), and the suction opening (108) is arranged at the top wall of the upper cabin body (101).

7. The centrifuge tube of claim 6, wherein: the upper cabin body (101) is further provided with a top spiral cover (5), the top spiral cover (5) is respectively provided with an injection outer opening (501) and a ventilation outer hole (502) corresponding to the injection opening (104) and the ventilation opening (105), a rubber outer plug (503) is arranged in the injection outer opening (501), and a bacterium blocking outer membrane (504) is arranged in the ventilation outer hole (502);

a second sealing element (6) is arranged between the end wall of the top spiral cover (5) and the outer pipe wall of the suction port (108), and a third sealing element (7) is arranged between the circumferential wall of the top spiral cover (5) and the outer circumferential wall of the upper bin body (101).

8. The centrifuge tube of claim 5, wherein: the injection opening (104) and the vent opening (105) are horizontally arranged on the edge of the top wall of the upper cabin body (101) in the form of a luer connector.

9. The centrifugal separation tube according to any one of claims 1 to 8, which is used for preparing platelet-rich plasma by one-time centrifugation.

10. A method for manufacturing a centrifugal separation tube, comprising the steps of:

step 1, integrally molding an outer tube body (1) which is through up and down and comprises an upper bin body (101), a throat bin (103) and a lower bin body (102);

step 2, ultrasonically welding or gluing the upper end of the central liquid taking pipe (2) on a suction opening (108) on the top wall of the upper bin body (101), and ultrasonically welding or gluing the lower end on the inner wall of the throat bin (103);

step 3, welding or cementing the top wall of the upper bin body (101) and the outer tube body (1) into a whole;

and 4, respectively assembling a rubber plug (106) and a bacteria-resisting film (107) on an injection port (104) and an air vent (105) on the upper bin body (101), and assembling a bottom cover (3) on the lower bin body (102).