CN111013197A - Disposable platelet-rich plasma separation device - Google Patents
Disposable platelet-rich plasma separation device Download PDFInfo
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- CN111013197A CN111013197A CN201911423964.6A CN201911423964A CN111013197A CN 111013197 A CN111013197 A CN 111013197A CN 201911423964 A CN201911423964 A CN 201911423964A CN 111013197 A CN111013197 A CN 111013197A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
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Abstract
The invention is used for realizing the aseptic disposable PRP separation of the whole process, relate to a disposable platelet-rich plasma separator specifically, it includes upper cover, body of the tube, lower cover, PRP tube, inner cannula and baffle, only need centrifuge finish separating once through the invention, does not need the transfer process of the plasma, it is more convenient to operate; because the blood does not need to be transferred, the blood is completely isolated from the outside in the whole process, and the possibility of pollution is fundamentally reduced; by the invention, PRP can be concentrated in the PRP cylinder, and the enrichment degree of platelets is high and can be up to about 50 times; meanwhile, the enrichment degree of the blood platelets can be automatically adjusted according to needs, and the operation is very convenient; in the operation process, the loss of platelets is less, the difference is not too large when different personnel operate, and the consistency of the quality of PRP can be ensured.
Description
Technical Field
The invention relates to a disposable platelet-rich plasma separation device, which is used for realizing sterile disposable PRP separation in the whole process.
Background
Platelet-rich plasma (PRP) is a medical component of autologous origin, which is naturally not rejected by the body and is safer to use. Platelets are key cellular components for maintaining the human coagulation balance and play a crucial role in hemostasis. However, besides the hemostatic function, platelets play an important role in wound healing, tissue regeneration, fracture healing, and the like, mainly because platelet cells not only contain factors related to blood coagulation, but also contain serotonin, platelet growth factor, transforming growth factor, and the like, in particular platelet growth factor and transforming growth factor, and after being released in large quantities by platelets, the platelets can induce surrounding cells to produce more factors, such as epidermal growth factor, fibroblast growth factor, and the like, and the factors act together, so that the wound healing and tissue regeneration speed can be greatly improved.
At present, PRP can be used for wound treatment during surgery, which can effectively reduce the amount of extra blood transfusion; some diabetic patients may suffer from wound healing disorders where some parts of them, especially wounds on the lower limbs and feet, fail to heal for a long time, and PRP can be used to help these patients very effectively; PRP can be used to promote healing of bone fractures, accelerate the recovery rate of bone injury; PRP can also be used to treat chronic joint injuries, such as some knee wear-type diseases, which can be effectively alleviated; the PRP is taken from the autologous material, has no threat in safety and no worry about any toxic and side effect, and is therefore widely popular.
At present, most of PRP preparation methods popular in the market are secondary centrifugation methods, namely after blood is drawn, firstly, red blood cells, plasma and platelets are separated into layers through first short-time centrifugation (the process is usually 1-2 minutes), then, the plasma and the platelets are transferred to a new container to be subjected to second long-time centrifugation, and the platelets are enriched at the bottom, so that platelet-rich plasma PRP and poor platelet plasma PPP are obtained. The method is complicated in operation and difficult to control, and PRP obtained by different operators has large content difference of platelets and low concentration of the platelets, and the concentration of the platelets is only 2-4 times of that of the platelets in whole blood. More importantly, during the transferring process of the secondary centrifugation, bacterial pollution is likely to be caused, and safety threat is likely to be caused to patients.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: overcomes the defects in the prior art, and provides the disposable platelet-rich plasma separation device which does not need to transfer plasma, is more convenient to operate, is completely isolated from the outside in the whole blood process, and fundamentally reduces the possibility of pollution.
The invention relates to a disposable platelet-rich plasma separation device, which comprises an upper cover, a cylinder body, a lower cover, a PRP cylinder, an inner sleeve and a baffle plate, wherein the upper cover is arranged on the cylinder body; the upper cover and the lower cover are respectively connected with the cylinder body from the upper end and the lower end to form a cylinder body in a sealing way, both the upper cover and the lower cover can generate relative displacement with the cylinder body, the upper cover is provided with a blood injection hole and an air inlet, and the air inlet is provided with a breathable filter membrane; the barrel body is made of transparent materials, the diameter of an inner cavity of the middle section is smaller than the diameters of an upper cavity and a lower cavity in the barrel body, a slot is transversely formed in the lower portion of the middle section, the inner end of the baffle is inserted into the slot from outside to inside, a hollow groove is formed in the middle of the baffle, the outer end of the baffle is a drawing operation end, a sampling opening is formed in the side face of the middle section, and a plugging piece is installed at the sampling opening; the inner sleeve is made of elastic transparent materials, is installed in the middle section and is in interference fit with the middle section, the inner sleeve penetrates through a hollow groove of the baffle (namely the inner sleeve penetrates through the baffle along the thickness direction of the baffle), the part of the baffle adjacent to the inner sleeve is a cutting part, when the drawing operation end is pulled outwards, the cutting part cuts the inner sleeve, and the baffle can divide the inner cavity of the inner sleeve into two parts which are mutually independent from each other up and down; the PRP cylinder is made of transparent materials, is positioned in the upper cavity and is coaxial with the inner sleeve, the upper end of the PRP cylinder is fixed on the upper cover, and the upper part of the PRP cylinder is provided with a vent hole; in the initial state, a space is reserved between the lower end of the PRP cylinder and the inner sleeve, when the PRP cylinder moves downwards, the lower end of the PRP cylinder can be compacted on the inner wall of the inner sleeve, the inner cavity of the PRP cylinder and the inner cavity of the inner sleeve are connected into a whole, and the lowest end of the PRP cylinder is higher than the sampling port.
The disposable platelet-rich plasma separation device can realize the sterile disposable PRP separation in the whole process, and the PRP separation method adopts the traditional centrifugation method and comprises the following specific steps:
① the collected blood with anticoagulant is injected into the upper cavity of the barrel through the blood injection hole, then the whole device is placed in a swing rotor centrifuge for centrifugation, the blood is divided into 3 layers after the centrifugation, the uppermost layer is a plasma layer which accounts for 50-65% of the total volume, the middle layer is a leucocyte layer which mainly contains thrombocytes and leucocytes and accounts for less than 1% of the total volume, and the lowest layer is a erythrocyte layer which accounts for 35-50% of the total volume.
② moving the lower cover to the barrel side (i.e. moving the lower cover upwards) to move the red blood cell interface upwards until the red blood cell interface approaches the top surface of the lower cavity of the barrel, moving the upper cover to the barrel side (i.e. moving the upper cover downwards), compacting the PRP barrel and the inner sleeve into a whole, connecting the inner cavities of the PRP barrel and the inner sleeve into a whole, then continuously moving the lower cover upwards, carefully adjusting the red blood cell interface to be flush with the lower surface of the baffle;
③ the pulling operation end of the baffle is pulled slowly to be drawn outwards until the cutting part cuts the inner sleeve and the baffle divides the inner cavity of the inner sleeve into two parts which are independent from each other, at the moment, red blood cells are arranged below the baffle, PRP and a small amount of plasma are arranged in the PRP cylinder above the baffle, and plasma is arranged outside the PRP cylinder above the baffle.
④ the stopper at the sampling port was pulled out, and the inner cannula was pierced through the sampling port with a syringe, carefully withdrawing all of the substance (mixture of PRP and small amount of plasma) from the PRP cartridge.
If more plasma is needed, the upper cover can be moved upwards to separate the lower end of the PRP cylinder from the inner sleeve, the plasma in situ outside the PRP cylinder flows into the inner sleeve and the PRP cylinder, and the plasma with the required volume is extracted, so that the concentration of the platelets can be automatically regulated.
According to the invention, the matching design of the PRP cylinder and the inner sleeve is very critical, and the operation steps are known, blood added with anticoagulant is divided into a plasma layer, a white membrane layer and a red blood cell layer from top to bottom after centrifugal operation, after the red blood cell interface is adjusted to be close to the top surface of the lower cavity of the cylinder body (namely close to the bottom surface of the inner sleeve), the white membrane layer with lower volume ratio is basically concentrated in the inner sleeve, the PRP cylinder moves downwards and is compacted with the inner sleeve, the inner cavities of the PRP cylinder and the inner sleeve are connected into a whole, and when the red blood cell interface is adjusted upwards to be level with the lower surface of the baffle, the PRP is positioned in the inner; meanwhile, the inner diameters of the inner sleeve and the PRP cylinder are smaller (the inner diameter of the inner sleeve is smaller due to the fact that the inner sleeve is in interference fit in the middle section with the smaller diameter, and the inner diameter of the inner sleeve is smaller, so that the inner wall of the inner sleeve can be compacted, the inner diameter of the PRP cylinder with the inner cavity communicated with the inner cavity of the inner sleeve is smaller), plasma content in the inner sleeve above the baffle and in the PRP cylinder is smaller, and therefore the platelet enrichment degree is high and can be enriched by about 50 times at most. Meanwhile, the PRP cylinder is moved upwards, plasma with the required volume can be extracted from the sampling port, so that the platelet enrichment degree can be automatically adjusted according to the requirement, and the operation is very convenient.
The device can complete separation only by one-time centrifugation, does not need the transfer process of plasma, and is more convenient to operate; because the blood does not need to be transferred, the blood is completely isolated from the outside in the whole process, and the possibility of pollution is fundamentally reduced.
In addition to the above, in order to keep the whole device working normally, the upper cover is provided with an air inlet which is provided with a ventilating filter membrane and can filter the pollution particles such as bacteria and the like in the air; the upper end of the PRP cylinder is also provided with two small vent holes to ensure the air pressure balance in the PRP cylinder. The sampling port is plugged by the plugging piece, so that the pollution in the centrifugal process can be effectively prevented; in an actual product, the blocking piece can be connected with the baffle plate through a section of flexible connecting belt (at this time, the flexible connecting belt is the drawing operation end). The barrel, the upper cover and the lower cover should have high strength to ensure that no breakage occurs during centrifugation. The cylinder body is made of transparent materials, so that the condition of internal blood can be seen; the inner sleeve is made of transparent elastic material, and can be made of PVC material or transparent rubber with high transparency, so that the inner sleeve is easily cut by the cutting part of the baffle, and the blood condition in the inner cavity and the actual moving position of the baffle in the process of cutting the inner sleeve are conveniently observed; the PRP cylinder is also made of transparent materials, so that a user can clearly observe the liquid level change in the PRP cylinder, and the extraction process of the PRP finished product can be conveniently controlled. In order to be able to clearly observe the displacement position of the flap, the flap body is preferably designed as an opaque and distinctly colored plastic. The cutting part of baffle can adopt stainless steel, it imbed in the baffle can, in order to realize the smooth cutting to interior sleeve pipe, the cutting edge of cutting part can be designed into the cutting sharp sword of going up the slope and going down flat, like this, when outwards pulling the baffle, the interior sleeve pipe can be cut easily to the cutting sharp sword, realizes the isolation of erythrocyte and PRP.
From the above, the device of the present invention has the following advantages:
1. the separation can be completed only by one-time centrifugation, the transfer process of the plasma is not needed, and the operation is more convenient;
2. because the blood does not need to be transferred, the blood is completely isolated from the outside in the whole process, and the possibility of pollution is fundamentally reduced;
3. by the invention, PRP can be concentrated in the PRP cylinder, and the enrichment degree of platelets is high and can be up to about 50 times; meanwhile, the enrichment degree of the platelets can be adjusted according to needs, after all substances in the PRP cylinder are pumped out, the PRP cylinder is moved upwards, the plasma can enter the inner sleeve and the PRP cylinder, the plasma with the required volume is pumped out, the concentration of the platelets can be automatically adjusted, and the operation is very convenient;
4. the inner sleeve and the PRP cylinder are small in inner diameter, and even if platelets with a certain height are lost when the inner sleeve and the PRP cylinder are isolated by the baffle, the ratio of the inner sleeve to the PRP cylinder is low.
5. Because of convenient operation and less platelet loss, the difference is not too large when different personnel operate, and the consistency of the PRP quality (namely the uniformity or consistency of different batches or personnel operation is high) can be ensured.
Preferably, the middle section of the cylinder body is a necking section; in the barrel body, the bottom of the upper cavity is in an inverted cone shape, and the top of the lower cavity is in a cone shape. Through this structural design, can be convenient for concentrate on necking down section position with the less white rete of volume ratio.
Preferably, the outer wall of the inner jacket tube is annularly cut to form a cutting groove at a horizontal position corresponding to the cutting part, and the wall thickness at the annular cutting position is d1. Further preferably, the wall thickness d at the ring-cut location1The value range of (1) is more than 0 and less than d1Less than or equal to 1mm, the thin inner sleeve can be easily cut through the cutting part of the baffle plate, thereby being convenient to realizeAnd cutting the inner sleeve to finally realize the isolation of the red blood cells and the PRP.
Preferably, an inner groove is formed in the outer wall of the inner sleeve at a position corresponding to the sampling port; wall thickness d of the location of the inner groove2The value range of (1) is more than 0 and less than d2Less than or equal to 2 mm. The smaller the wall thickness of the position of the inner sleeve corresponding to the sampling port is, the easier the injector pierces the inner sleeve, and the PRP is convenient to extract.
Preferably, the lower end of the PRP barrel is a reverse taper having an outer diameter gradually decreasing, and the inner diameter of the inner sleeve is greater than the minimum outer diameter of the lower end of the PRP barrel and less than the maximum outer diameter of the lower end of the PRP barrel. When the PRP cylinder moves downwards, the lower end of the PRP cylinder can be completely compacted on the inner wall of the inner sleeve to ensure that the inner cavities of the PRP cylinder and the inner sleeve are communicated into a whole.
Preferably, the baffle is provided with an anti-return structure. Further preferably, the anti-returning structure is elastic arms arranged on two sides of the baffle, the end, close to the slot, of each elastic arm is a free end, and when the baffle moves outwards along the slot in the middle section until the elastic arms are completely separated from the slot, the two elastic arms pop out (namely the free ends of the elastic arms are in an outwards-opened state), so that the baffle cannot return. Further preferably, when the baffle plate moves outwards along the slot of the middle section until the elastic arm is completely separated from the slot, the baffle plate just divides the inner cavity of the inner sleeve into two parts which are mutually independent from each other up and down, and through the design, an operator can judge that the baffle plate reaches a required position without observing by eyes (the elastic arm can make a 'click' when being popped out, and can judge by hearing the sound, and the operator can feel the popping of the elastic arm when pulling the baffle plate outwards by hands.
Preferably, the upper cover, the lower cover and the cylinder body are connected through threads. The butt joint screw thread of upper cover, lower cover and stack shell should guarantee very smoothly when rotatory, should not consume the strong strength or have the sense of hesitation, and first make things convenient for rotatory adjustment interface, second prevent to exert oneself and change suddenly when adjusting the interface and produce the vibration, make the interface receive the upset. In the actual product, multiplicable sealing washer between lower cover and the stack shell or directly make the lower cover adopt the rubber material to make the leakproofness of guaranteeing that lower cover and stack shell are connected, do not have when guaranteeing the centrifugation and reveal.
Compared with the prior art, the invention has the beneficial effects that:
1. the separation can be completed only by one-time centrifugation, the transfer process of the plasma is not needed, and the operation is more convenient;
2. the whole process of the blood is completely isolated from the outside, thus fundamentally reducing the possibility of pollution;
3. by the invention, PRP can be concentrated in the PRP cylinder, and the enrichment degree of platelets is high and can be up to about 50 times; meanwhile, the enrichment degree of the blood platelets can be automatically adjusted according to needs, and the operation is very convenient;
4. and the layering with red blood cells is convenient, the pollution of red blood cells can be reduced, the loss of platelets can be reduced, and due to the convenient operation and the low loss of platelets, the difference is not too large when different personnel operate, and the consistency of the quality of PRP can be ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is a schematic view of the barrel construction;
FIG. 4 is a schematic structural view showing the connection of the blocking member to the shutter by a flexible connecting band in the embodiment;
FIG. 5 is a schematic view of the inner sleeve;
fig. 6 is a cross-sectional view of the inner sleeve.
In the figure: 1. an upper cover; 2. a barrel body; 3. a middle section; 4. a lower cover; 5. a baffle plate; 6. a bendable connecting belt; 7. a blood injection hole; 8. an air inlet; 9. a PRP cartridge; 10. an upper cavity; 11. an inner sleeve; 12. a blocking member; 13. a lower cavity; 14. a cutting section; 15. a vent hole; 16. a gas-permeable filter membrane; 17. a slot; 18. a sampling port; 19. an empty groove; 20. a resilient arm; 21. an inner groove; 22. and (6) grooving.
Detailed Description
Embodiments of the invention are further described below with reference to the accompanying drawings:
as shown in fig. 1 to 6, the disposable platelet-rich plasma separation device of the present embodiment includes an upper cover 1, a cylinder body 2, a lower cover 4, a PRP cylinder 9, an inner sleeve 11, and a baffle 5;
the upper cover 1 and the lower cover 4 are respectively connected with the cylinder body 2 from the upper end and the lower end to form a cylinder body in a sealing way, in the embodiment, the upper cover 1, the lower cover 4 and the cylinder body 2 are connected through threads, and when the threads are connected, the smooth rotation is ensured, the large strength or the sluggish feeling is not consumed, so that the interface is conveniently adjusted by rotation, and the vibration caused by sudden change of the force during the adjustment of the interface is prevented, so that the interface is disturbed; the lower cover 4 is made of rubber materials so as to ensure the sealing property of the connection between the lower cover 4 and the cylinder body 2 and ensure no leakage during centrifugation; the upper cover 1 is provided with a blood injection hole 7 and an air inlet 8, and the air inlet 8 is provided with a ventilation filter membrane 16 which can filter the pollution particles such as bacteria and the like in the air;
the cylinder body 2 is made of transparent material, and the diameter of the inner cavity of the middle section 3 is smaller than the diameters of the upper cavity 10 and the lower cavity 13 in the cylinder body 2; in the embodiment, the middle section 3 of the cylinder body 2 is a necking section, the bottom of the upper cavity 10 in the cylinder body 2 is in an inverted cone shape, and the top of the lower cavity 13 in the cylinder body 2 is in a cone shape, so that the white film layer with small volume ratio can be conveniently concentrated at the position of the necking section through the structural design;
a slot 17 is transversely formed in the lower part of the middle section 3 (in the embodiment, the slot 17 does not penetrate through the middle section 3, and one end of the slot is closed), the inner end of the baffle 5 is inserted into the slot 17 from outside to inside, a hollow groove 19 is formed in the middle position of the baffle 5, the outer end of the baffle 5 is a pulling operation end, a sampling port 18 is formed in the side surface of the middle section 3, and a plugging piece 12 is installed at the sampling port 18 to prevent pollution in the centrifugal process;
the inner sleeve 11 is made of elastic transparent material (selected PVC material or transparent rubber with high transparency, which is easily cut by the cutting portion 14 of the baffle 5, and is convenient for observing the blood condition in the inner cavity and the actual moving position of the baffle 5 in the process of cutting the inner sleeve 11), is installed in the middle section 3 and is in interference fit with the middle section 3, the inner sleeve 11 passes through the empty slot 19 of the baffle 5 (namely, the inner sleeve 11 passes through the baffle 5 along the thickness direction of the baffle 5), the cutting portion 14 is arranged at the part of the baffle 5 adjacent to the inner sleeve 11, and the cutting portion 14 is arranged at the part of the cutting portion 14The cutting edge is a cutting sharp edge which is inclined upwards and flat downwards (the cutting part 14 can be made of stainless steel, and one end of the cutting part is embedded into the baffle 5 to realize the fixed connection with the baffle 5), and when the baffle 5 is pulled outwards, the inner sleeve 11 can be easily cut through the cutting sharp edge; on the outer wall of the inner tube 11, a cutting groove 22 is formed by circular cutting at a horizontal position corresponding to the cutting part 14, and the wall thickness d at the circular cutting position1Is 1 mm; meanwhile, the outer wall of the inner sleeve 11 is provided with an inner groove 21 at a position corresponding to the sampling port 18, and the wall thickness d of the position of the inner groove 21 is21.5mm, the syringe penetrates the inner cannula 11 through the location of the inner recess 21 to withdraw the PRP;
the baffle 5 is provided with an anti-rollback structure, the anti-rollback structure in this embodiment is an elastic arm 20 arranged on both sides of the baffle 5, the end of the elastic arm 20 near the slot 17 is a free end, when the baffle 5 moves outwards along the slot 17 of the middle section 3 until the elastic arm 20 is completely separated from the slot 17, the two elastic arms 20 pop out (i.e. the free ends of the elastic arms 20 are in an outwards-opened state), so that the baffle 5 cannot roll back; when the drawing operation end is pulled outwards, the cutting part 14 cuts the inner sleeve 11, when the elastic arm 20 is popped out, the baffle 5 just divides the inner cavity of the inner sleeve 11 into two parts which are mutually independent from each other up and down, and through the design, an operator can judge that the baffle 5 reaches a required position without observing by eyes (the elastic arm 20 can give out a 'click' when being popped out, and can judge by hearing the sound; the operator can feel the popping of the elastic arm 20 when pulling the baffle 5 outwards by hands);
in order to clearly observe the moving position of the baffle 5, the baffle 5 is preferably made of opaque plastic with obvious colors;
the PRP cylinder 9 is made of transparent materials, is positioned in the upper cavity 10 and is coaxial with the inner sleeve 11, the upper end of the PRP cylinder is fixed on the upper cover 1, and the upper part of the PRP cylinder 9 is provided with two small vent holes 15 so as to ensure the air pressure balance in the PRP cylinder 9; the lower end of the PRP cylinder 9 is in an inverted cone shape with gradually reduced outer diameter, and the inner diameter of the inner sleeve 11 is larger than the minimum outer diameter of the lower end of the PRP cylinder 9 and smaller than the maximum outer diameter of the lower end of the PRP cylinder 9; in an initial state, a space is reserved between the lower end of the PRP cylinder 9 and the inner sleeve 11, when the PRP cylinder 9 moves downwards, the lower end of the PRP cylinder can be completely compacted on the inner wall of the inner sleeve 11, and the inner cavities of the PRP cylinder and the inner sleeve are communicated into a whole; the lowest end of the PRP cartridge 9 is located higher than the sampling port 18. In this embodiment, the PRP cartridge 9 has an inner diameter of 5mm, the volume of the inner cavity of the PRP cartridge 9 is about 1.2ml, and the inner diameter of the inner cannula 11 is 5 mm.
The disposable platelet-rich plasma separation device can realize the sterile disposable PRP separation in the whole process, and the PRP separation method adopts the traditional centrifugation method and comprises the following specific steps:
① the collected blood added with anticoagulant is injected into the upper cavity 10 of the barrel 2 through the blood injection hole 7, then the whole device is placed in a centrifugal machine of a swing rotor for centrifugation, the blood is divided into 3 layers after the centrifugation, the uppermost layer is a plasma layer which accounts for 50-65% of the total volume, the middle layer is a leucocyte layer which mainly contains blood platelets and leucocytes and accounts for less than 1% of the total volume, and the lowest layer is a red blood cell layer which accounts for 35-50% of the total volume.
② rotating the lower cover 4 to move the lower cover 4 to the barrel 2 side (i.e. the lower cover 4 moves upwards) to move the red blood cell interface upwards until the red blood cell interface approaches the conical top surface of the lower cavity 13 of the barrel 2, then rotating the upper cover 1 to move the upper cover 1 to the barrel 2 side (i.e. the upper cover 1 moves downwards), compacting the PRP barrel 9 and the inner sleeve 11 to integrate the inner cavities of the two, then continuing to rotate the lower cover 4 to move upwards, carefully adjusting the red blood cell interface to be flush with the lower surface of the baffle 5;
③ the stopper 12 blocking the sampling port 18 is carefully pulled out, then the flexible connecting band 6 is pulled to slowly pull the baffle 5 outwards, the cutting edge of the cutting part 14 of the baffle 5 cuts the inner sleeve 11 along the circular cutting position until the sound of 'Da' is heard, the elastic arms 20 at the two sides of the baffle 5 are ejected to make the baffle 5 unable to retreat, at this time, red blood cells are below the baffle 5, PRP and a small amount of plasma are in the PRP cylinder 9 above, and plasma is outside the PRP cylinder 9 above.
④ the inner cannula 11 is pierced by the sampling port 18 with a 5ml syringe and the contents of the PRP cartridge 9 (mixture of PRP and a small amount of plasma) are carefully withdrawn in their entirety.
If more plasma is needed, the upper cover 1 can be moved upwards, the lower end of the PRP cylinder 9 is separated from the inner sleeve 11, the plasma in situ outside the PRP cylinder 9 flows into the inner sleeve 11 and the PRP cylinder 9, and the required volume of plasma is extracted, so that the concentration of the platelets can be automatically regulated.
As can be seen from the above, the device of the present embodiment has the following advantages:
1. the separation can be completed by only one-time centrifugation through the device, the transfer process of the plasma is not needed, and the operation is more convenient;
2. because the blood does not need to be transferred, the blood is completely isolated from the outside in the whole process, and the possibility of pollution is fundamentally reduced;
3. by the device, the PRP can be concentrated in the PRP cylinder 9, the enrichment degree of the blood platelet is high, and the enrichment can be about 50 times at most; meanwhile, the enrichment degree of the platelets can be adjusted according to needs, after all substances in the PRP cylinder 9 are pumped out, the PRP cylinder 9 is moved upwards, the plasma can enter the inner sleeve 11 and the PRP cylinder 9, the plasma with the required volume is pumped out, the concentration of the platelets can be automatically adjusted, and the operation is very convenient;
4. the inner sleeve 11 and the PRP cylinder 9 have small inner diameters (0.5ml is about 2.5cm higher in the PRP cylinder 9), and even if platelets with a certain height are lost when the inner sleeve and the PRP cylinder are separated by the baffle 5, the ratio of the inner sleeve to the inner sleeve is low.
5. Because the operation is convenient and the loss of the blood platelet is less, the difference is not too large when different personnel operate, and the consistency of the PRP quality can be ensured.
Claims (10)
1. A disposable platelet-rich plasma separation device is characterized in that: comprises an upper cover (1), a cylinder body (2), a lower cover (4), a PRP cylinder (9), an inner sleeve (11) and a baffle (5);
the upper cover (1) and the lower cover (4) are respectively connected with the barrel body (2) from the upper end and the lower end to form a barrel body in a sealing way, the upper cover (1) and the lower cover (4) can be in relative displacement with the barrel body (2), the upper cover (1) is provided with a blood injection hole (7) and an air inlet (8), and the air inlet (8) is provided with a breathable filter membrane (16);
the barrel body (2) is made of transparent materials, the diameter of an inner cavity of the middle section (3) is smaller than the diameters of an upper cavity (10) and a lower cavity (13) in the barrel body (2), the lower part of the middle section (3) is transversely provided with a slot (17), the inner end of the baffle (5) is inserted into the slot (17) from outside to inside, the middle position of the baffle (5) is provided with a hollow groove (19), the outer end of the baffle (5) is a drawing operation end, the side surface of the middle section (3) is provided with a sampling port (18), and a plugging piece (12) is arranged at the sampling port (18);
the inner sleeve (11) is made of elastic transparent materials and is installed in the middle section (3) and in interference fit with the middle section (3), the inner sleeve (11) penetrates through a hollow groove (19) of the baffle (5), the part, adjacent to the inner sleeve (11), of the baffle (5) is a cutting part (14), when the drawing operation end is pulled outwards, the cutting part (14) cuts the inner sleeve (11), and the baffle (5) can divide the inner cavity of the inner sleeve (11) into two parts which are independent from each other up and down;
the PRP cylinder (9) is made of transparent materials, is positioned in the upper cavity (10) and is coaxial with the inner sleeve (11), the upper end of the PRP cylinder is fixed on the upper cover (1), and the upper part of the PRP cylinder (9) is provided with a vent hole (15); in an initial state, a space is reserved between the lower end of the PRP cylinder (9) and the inner sleeve (11), when the PRP cylinder moves downwards, the lower end of the PRP cylinder can be compacted on the inner wall of the inner sleeve (11), the inner cavity of the PRP cylinder (9) and the inner cavity of the inner sleeve (11) are connected into a whole, and the lowest end of the PRP cylinder (9) is higher than the position of the sampling port (18).
2. The disposable platelet rich plasma separation device of claim 1 wherein: the middle section (3) of the cylinder body (2) is a necking section; in the cylinder body (2), the bottom of the upper cavity (10) is in an inverted cone shape, and the top of the lower cavity (13) is in a cone shape.
3. The disposable platelet-rich plasma separation device according to claim 1 or 2, wherein: on the outer wall of the inner sleeve (11), a cutting groove (22) is formed by circular cutting at the horizontal position corresponding to the cutting part (14), and the wall thickness of the circular cutting position is d1。
4. The disposable platelet rich plasma separation device of claim 3 wherein: wall thickness d at the circumferential cutting position1The value range of (1) is more than 0 and less than d1≤1mm。
5. The disposable platelet-rich blood according to claim 1 or 2The thick liquid separator, its characterized in that: an inner groove (21) is formed in the outer wall of the inner sleeve (11) at a position corresponding to the sampling port (18); the wall thickness d of the inner groove (21) is2The value range of (1) is more than 0 and less than d2≤2mm。
6. The disposable platelet-rich plasma separation device according to claim 1 or 2, wherein: the lower end of the PRP cylinder (9) is in an inverted cone shape with the outer diameter gradually reduced, and the inner diameter of the inner sleeve (11) is larger than the minimum outer diameter of the lower end of the PRP cylinder (9) and smaller than the maximum outer diameter of the lower end of the PRP cylinder (9).
7. The disposable platelet rich plasma separation device of claim 1, 2 or 4, wherein: the baffle (5) is provided with an anti-return structure.
8. The disposable platelet rich plasma separation device of claim 7, wherein: the anti-return structure is an elastic arm (20) arranged on two sides of the baffle (5), and the end of the elastic arm (20) close to the slot (17) is a free end.
9. The disposable platelet rich plasma separation device of claim 8 wherein: when the baffle (5) moves outwards along the slot (17) of the middle section (3) until the elastic arm (20) is completely separated from the slot (17), the baffle (5) just divides the inner cavity of the inner sleeve (11) into two parts which are mutually independent from each other up and down.
10. The disposable platelet rich plasma separation device of claim 1, 2, 4, 8 or 9 wherein: the upper cover (1), the lower cover (4) and the barrel body (2) are connected through threads.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911423964.6A CN111013197A (en) | 2019-12-31 | 2019-12-31 | Disposable platelet-rich plasma separation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911423964.6A CN111013197A (en) | 2019-12-31 | 2019-12-31 | Disposable platelet-rich plasma separation device |
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| CN111013197A true CN111013197A (en) | 2020-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911423964.6A Withdrawn CN111013197A (en) | 2019-12-31 | 2019-12-31 | Disposable platelet-rich plasma separation device |
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| CN (1) | CN111013197A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111974475A (en) * | 2020-08-20 | 2020-11-24 | 绵阳晶凯圣生物科技有限公司 | Blood component separator |
| CN114146827A (en) * | 2021-11-26 | 2022-03-08 | 南京双威生物医学科技有限公司 | One-time centrifugal preparation method of platelet-rich plasma |
| CN114160226A (en) * | 2021-11-26 | 2022-03-11 | 南京双威生物医学科技有限公司 | Centrifugal separation tube and manufacturing method and application thereof |
| CN114870436A (en) * | 2022-06-21 | 2022-08-09 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN114887681A (en) * | 2022-06-13 | 2022-08-12 | 杭州博科迈生物科技有限公司 | Platelet-rich plasma separation device |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
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2019
- 2019-12-31 CN CN201911423964.6A patent/CN111013197A/en not_active Withdrawn
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111974475A (en) * | 2020-08-20 | 2020-11-24 | 绵阳晶凯圣生物科技有限公司 | Blood component separator |
| CN114146827A (en) * | 2021-11-26 | 2022-03-08 | 南京双威生物医学科技有限公司 | One-time centrifugal preparation method of platelet-rich plasma |
| CN114160226A (en) * | 2021-11-26 | 2022-03-11 | 南京双威生物医学科技有限公司 | Centrifugal separation tube and manufacturing method and application thereof |
| WO2023093781A1 (en) * | 2021-11-26 | 2023-06-01 | 南京双威生物医学科技有限公司 | Centrifugal separation tube and manufacturing method therefor and use thereof |
| CN114146827B (en) * | 2021-11-26 | 2024-01-23 | 南京双威生物医学科技有限公司 | One-time centrifugation preparation method of platelet-rich plasma |
| CN114887681A (en) * | 2022-06-13 | 2022-08-12 | 杭州博科迈生物科技有限公司 | Platelet-rich plasma separation device |
| CN114870436A (en) * | 2022-06-21 | 2022-08-09 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN114870436B (en) * | 2022-06-21 | 2024-08-06 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN117126716A (en) * | 2023-09-06 | 2023-11-28 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
| CN117126716B (en) * | 2023-09-06 | 2024-04-05 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
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