KR101170146B1 - Seperator and collection apparatus for extracting of platelet rich plasma - Google Patents

Abstract

PURPOSE: A blood separation and concentration device for extracting a growth factor in which the platelets are abundant is provided to apply wide range of blood injection amount and concurrently process separation and concentration processes using one container. CONSTITUTION: A blood separation and concentration device for extracting a growth factor in which the platelets are abundant comprises a bottom body(100), a top body(200), and a storage(300). In the bottom body, a space part is formed so that the blood can be accepted inside, a bottom cap which can adjust height is combined in order to change the volume of the space part, and a air permeable part which is connected to outside is formed at the upper part. A plug member is combined in the top of the bottom body and an acceptance space in which part of the separated blood is put inside. The storage is formed extended and formed at lower one-side of the top body.

Description

Separator and collection apparatus for extracting of Platelet Rich Plasma}

The present invention relates to a blood separation and concentration device for platelet-rich growth factor extraction, and in particular, the structure is improved to be able to apply a wide range of blood infusion as well as to separate and concentrate the operation in one container at the same time The present invention relates to a blood separation and concentration device for the extraction of platelet-rich growth factors.

Blood circulating in blood vessels in a human or animal body carries oxygen taken from the lungs to tissue cells, transports carbon dioxide from the tissues to the lungs for release, and transports nutrients absorbed from the digestive tract to organs or tissue cells, As a decomposition product of, it transports substances that are unnecessary to the living body to the kidneys and discharges them out of the body, transports hormones secreted from the endocrine glands to the organs and tissues, distributes body heat evenly, maintains constant body temperature, and invades the living body. It performs various functions such as destroying and detoxifying bacteria and foreign substances.

Although such blood is used as an important indicator for determining various diseases or health conditions, platelets rich in growth factors (Growth factor) in the blood are used for therapeutic purposes.

Blood is composed of red blood cells, white blood cells, platelets, etc. Among them, platelets are mainly present in plasma, and plasma is divided into a platelet rich plasma (PRP) layer and a plate poor plasma (PPP) layer.

Platelet-rich plasma, called Platelet Rich Plasma (PRP), is located below the plasma and contains growth factors such as cytokines, PDGF, TGF-BETA1, and VEGP, especially for skin diseases and wounds. A good effect on healing is found in the literature.

Platelet-free plasma, also called PPP (Platelet Poor Plasma), is used as an autologous filler or a cosmetic product derived from autologous plasma.

PRP layer is only about 1% of the collected blood and the viscosity is high, it is difficult to collect because it is attached to red blood cells. PRP is implanted in the pain area, especially the knee restraint, ligaments and muscles, and it is used for therapeutic purposes because it stimulates the stem cells around and helps to generate the cells. Sampling was difficult, and red blood cells entered the human body, causing considerable pain, which could cause inflammation, and the emphasis was on the technique of collecting concentrated platelets (PRP), except red blood cells.

However, in order to extract the existing thick platelet layer, the first and second centrifugation processes have a process of concentrating, and this process is performed after injecting the blood into the lower liquid chamber of the primary separation vessel having the upper and lower liquid chambers. The injected primary separation vessel is first centrifuged, and the leukocytes and buffy coat layer separated in the first centrifugation process are pushed to the upper liquid chamber.

Thereafter, the leukocytes and buffy coat layer of the upper liquid chamber are transferred to the secondary separation vessel side, and further centrifuged and concentrated to extract concentrated platelets.

At this time, not only does the loss occur in the process of transferring the separation layer of the first centrifuged blood to the secondary separation vessel side, but the work is cumbersome and takes a long time.

In addition, in the process of injecting the existing blood into the primary separation vessel has a process of separating the buffy coat layer and erythrocytes by selecting a container having a volume suitable for each blood dose, so that the volume suitable for each blood dose There was a hassle to have a container.

When the amount of blood is injected, a small volume container should be used. On the contrary, when the amount of blood is injected, a large volume container is used to separate and separate the buffy coat layer and the red blood cell layer which are separated during the centrifugation process. It is to make it easier.

The present invention was created in view of the above-mentioned problems, and the object thereof is to simplify the work process by allowing the first and second centrifugal separation processes for extracting and collecting concentrated platelets into one container. The present invention provides a blood separation and concentration device for extracting platelet-rich growth factors whose structure has been improved to increase the convenience of work.

Another object of the present invention is a blood separation and concentration device for platelet-rich growth factor extraction is improved in its structure to enable a wide range of blood injection irrespective of the amount of blood injection and the separation and concentration of the injected blood in one container To provide.

The present invention for achieving the above object is formed with a space portion to accommodate the blood therein, the lower cap is adjustable to change the height to change the volume of the space portion at the bottom, the through portion communicating with the outside on the top A lower body formed;

The receiving space is coupled to be seated on the upper side of the lower body to communicate, the stopper member for opening and closing the through-hole in the upper portion is liftable, the receiving space is accommodated in the separation layer of the blood separated after the first centrifugal separation therein An upper body having a;

And a storage unit extending in one lower portion of the upper body, communicating through an inflow hole formed in the bottom surface of the upper body, and receiving the concentrated platelets after the second centrifugal separation.

 The bottom surface of the upper body is formed to be inclined downward toward the inlet hole.

The lower cap is coupled to the inner circumferential surface of the lower body and the first cap member which is elevated when rotated, and coupled to the upper portion of the first cap member to the volume of the space portion of the lower body during the lifting operation of the first cap member It is provided with a second cap member of the silicon material for shrinking.

 The stopper member is coupled to penetrate through the upper cap disposed in the upper portion of the upper body and the outer circumferential surface is screwed to the upper cap to move up and down by a rotation operation to open and close the through-hole.

Removably coupled to the lower portion of the reservoir, and has an extraction stopper is formed in the center is formed with an outlet for entering the syringe.

A support piece for supporting the extraction plug from the lower side is formed on the upper portion of the lower body.

More preferably, the amount of concentrated platelets extracted through the outlet is varied according to the height of the extraction plug coupled to the lower portion of the reservoir.

The present invention has the following effects.

First, since the storage unit is formed on one side lower portion of the upper body, the separation and concentration work through centrifugal separation of blood is made through a single container to prevent the loss caused in the existing transfer process, as well as the convenience of work It has an effect that can increase.

Second, the present invention has the advantage that the first and second centrifugation and concentration can be made in a single container to expand the acceptable range of the infusion capacity of blood, thereby increasing the applicability.

Third, since the closure member of the present invention has a structure of opening and closing the through hole by the lifting operation, the convenience of the operation can be increased.

Fourth, it has a useful effect that can adjust the amount of extraction of the platelets in accordance with the binding height of the extraction stopper coupled to the bottom of the reservoir.

1 is a perspective view showing a blood separation and concentration apparatus for extracting platelet-rich growth factor according to the present invention.
Fig. 2 is an exploded perspective view of Fig. 1; Fig.
3 is a side view of FIG. 1;
Figure 4 is a cutaway perspective view of the device of the present invention.
Figures 5a, 5b, 5c, 5d, 5e is a use state diagram sequentially showing the use state of the present invention.

According to the present invention, after centrifugation is performed in a state in which the blood is contained in the lower body, leukocytes and platelets, which are separated layers of the centrifuged blood, are injected into the upper body, and then, second centrifugation is performed to collect concentrated platelets at a specific site. By doing so, the separation and concentration work can be carried out in one container at the same time.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Blood separation and enrichment apparatus for platelet-rich growth factor extraction according to the present invention, when described with reference to Figs. 1 to 5, the configuration is formed in the space portion 105 to receive blood therein is formed on the outside outside An accommodation space in which the lower body 100 is formed to communicate with the lower body 100 is formed, coupled to be seated on the upper side of the lower body 100 to communicate with each other, the separation layer of blood separated after the first centrifugation An upper body 200 having a 205 and a lower portion formed on one side of the upper body 200 is configured as a storage unit 300 for collecting concentrated platelets.

In more detail, the lower body 100 is coupled to the lower cap 120 is adjustable in height in order to change the volume of the space portion 105 in the lower portion.

In addition, the through hole 115 is formed in the center of the through hole 110 so that the white blood cells and platelets, which are separate layers of blood, are injected into the upper body 200 when the lower cap 120 rises.

The lower cap 120 is composed of the first and second cap members, the first cap member 122 has a structure in which a screw thread is formed on the outer circumferential surface to be raised and lowered upon rotation as it is screwed with the inner circumferential surface of the lower body 100. The second cap member 124 is bonded to the upper portion of the first cap member 122 as a silicon material to the volume of the space portion 105 of the lower body 100 during the lifting operation of the first cap member 122. Perform the function to reduce.

In this case, the first cap member 122 is rotated by the rotational force transmitted from a separate tool (drill) to have a structure that rises along the inner peripheral surface of the lower body (100).

On the other hand, the first cap member 122 of the lower cap 120 is not formed with a screw thread around the outer circumference, it may be raised by the lifting operation of an actuator (not shown) such as a cylinder.

In addition, a plurality of ring-shaped sealing members 125 are disposed around the outer circumferential surface of the second cap member 124 to reduce the volume of the space portion 105 at a uniform pressure during the ascending operation. It is preferable.

An upper cap 250 is coupled to an upper portion of the upper body 200, and a stopper member 400 for opening and closing the through hole 110 is liftably coupled to a center of the upper cap 250.

That is, the closure member 400 has a function of opening and closing the through hole 110 by the outer circumferential surface is screwed to the upper cap 250 is moved by the rotation operation.

In addition, the stopper member 400 is formed with a handle 402 to facilitate gripping.

The central lower portion of the upper body 200 has a structure in which the fitting portion 220 is formed so that the through-hole 110 of the lower body 100 is inserted into communication.

The storage unit 300 is detachably coupled to the extraction stopper 310 of the material that can be passed through the syringe 10 to extract the concentrated platelets collected using the syringe 10 or the like at the bottom.

Extraction plug 310 is formed in the outlet 312 of a thin material to enable the injection of the syringe in the center.

In addition, a plurality of support pieces 116 are provided on the upper surface of the lower body 100 so as to contact the lower surface of the extraction plug 310 when coupled with the upper body 200 so as to prevent the extraction plug 310 from being separated. It is formed to protrude.

At this time, the amount of the concentrated platelets stored in the storage unit 300 is changed according to the height of the extraction stopper 310 is coupled to the lower portion of the storage unit 300, and thus the concentration of the platelets extracted through the syringe 10 The amount of extraction can be adjusted.

That is, the storage unit 300 extends to one side lower portion of the upper body 200 and collects concentrated platelets concentrated after the second centrifugation of the blood separation layer accommodated in the receiving space 205 of the upper body 200. It has a structure in which a storage space is formed.

The storage space may vary in volume depending on the height at which the extraction stopper 310 is coupled, and may have a change in extraction amount (eg, 1.0 to 2.5cc) of concentrated platelets according to a change in height when the extraction stopper 310 is coupled.

To this end, the storage unit 300 has a structure in communication with the receiving space 205 of the upper body 200 through the inlet hole 212 formed in the bottom surface 210 of the upper body 200, the upper body 200 Bottom portion 210 of the other portion has a structure inclined downward toward the inlet hole (212).

This is to allow the concentrated platelets separated and concentrated in the second centrifugation to be collected into the storage unit 300 by the difference in specific gravity.

The storage unit 300 may be extracted to the outside after a predetermined volume of concentrated platelets is collected.

The operation of the present invention having such a configuration will now be described.

In the blood separation and enrichment apparatus for platelet-rich growth factor extraction according to the present invention, the blood is first injected into the space portion 105 of the lower body 100 (see FIG. 5A), and the first centrifugal centrifuge using a centrifuge. Separate.

At this time, the injection amount of blood may be injected about 30 ~ 50cc.

The injection process of the blood is injected into the space 105 of the lower body 100 through the plug member 400 and the through-hole 110 using the syringe 10.

In addition, at this time, the stopper member 400 is moved downward to seal the through-hole 115 of the through-hole 110, the receiving portion 205 of the upper body 200 and the space portion 105 of the lower body 100 It performs the function of blocking.

When the first centrifugal separation is completed, the blood contained in the space portion 105 of the lower body 100 is divided into red blood cells, white blood cells, platelets, and is separated in the order of white blood cells, platelets, red blood cells from the upper side according to the difference in specific gravity. See 5b)

Thereafter, the stopper member 400 is raised to communicate the space 105 of the lower body 100 and the receiving space 205 of the upper body 200, and then lower cap 120 of the lower body 100. When it is raised to reduce the volume in the space 105, the leukocytes and platelets of the separated layers of the first separated blood stored in the space 105 are transferred to the upper body 200 through the through holes 115 of the through holes 110. Is injected into the receiving space 205.

Subsequently, after the leukocytes and platelets are injected into the receiving space 205 of the upper body 200, the stopper member 400 is lowered again to close the through hole 110 (see FIG. 5C).

Thereafter, secondary centrifugation is performed using a centrifuge.

At this time, in the second centrifugation process, the plasma is highly concentrated to form concentrated platelets, and after being induced toward the inlet hole 212 along the bottom surface 210 of the upper body 200 inclined downward by the difference in specific gravity, It is stored in the storage space of the unit 300 (see Fig. 5d).

The dose of the concentrated platelets stored in the storage unit 300 varies depending on the amount of blood injected and the traits of the blood, and about 1.5 to 3.0 cc are concentrated and stored.

Thereafter, the user passes the syringe 10 through the outlet 312 of the extraction stopper 310 to enter the inside of the storage unit 300, and then moves the concentrated platelets PRP stored in the storage unit 300 to the outside. Perform the extraction (see Fig. 5e).

Due to this, the blood separation and enrichment apparatus for platelet-rich growth factor extraction of the present invention prevents the loss caused in the existing transport process because the separation and concentration operation through centrifugation of blood is performed through one container. In addition, it has the effect of increasing the convenience of work.

In addition, the present invention has the advantage that the applicability is increased because two centrifugations and concentrations can be made in one container to extend the allowable range of the infusion capacity of blood.

And by adjusting the height of the extraction stopper 310 has the advantage of controlling the amount of extraction of the platelets enriched.

Description of the Related Art [0002]
10: syringe 100: lower body
105: space portion 110: through hole
115: through-hole 116: support piece
120: lower cap 122,124: first and second cap member
125: sealing member 200: upper body
205: accommodation space 210: bottom surface (upper body)
212: inlet hole 220: fitting
250: upper cap 300: storage unit
310: extraction stopper 312: outlet
400: stopper member

Claims (7)

Space portion 105 is formed so that the blood is received therein, the lower cap 120 is adjustable to change the height of the lower portion to change the volume of the space portion 105, the through portion communicating with the outside on the top A lower body 100 in which 110 is formed;
It is seated on the upper side of the lower body 100 is coupled to communicate with, the stopper member 400 for opening and closing the through hole 110 is coupled to the liftable, the blood separated after the first centrifugal separation therein An upper body 200 having an accommodating space 205 in which a part of the separation layer is accommodated;
Is formed extending from one side lower portion of the upper body 200, and communicates through the inlet hole 212 formed in the bottom surface 210 of the upper body 200, the storage platelet is accommodated after the secondary centrifugation Part 300; Blood separation and concentration device for platelet-rich growth factor extraction, characterized in that provided with. The apparatus of claim 1, wherein the bottom surface 210 of the upper body 200 is formed to be inclined downward toward the inlet 212. The method of claim 1, wherein the lower cap 120 is screwed with the inner circumferential surface of the lower body 100 and the first cap member 122 which is elevated when rotating;
The second cap member of the silicon material is coupled to the upper portion of the first cap member 122 to reduce the volume of the space portion 105 of the lower body 100 during the lifting operation of the first cap member 122 ( 124) blood separation and enrichment apparatus for platelet-rich growth factor extraction, characterized in that it comprises a. The method of claim 1, wherein the stopper member 400 is coupled to penetrate through the upper cap 250 disposed in the upper portion of the upper body 200 and the outer peripheral surface is screwed to the upper cap 250 to elevate by the rotation operation A blood separation and concentration device for platelet-rich growth factor extraction, characterized in that the opening and closing the through-hole 110. According to any one of claims 1 to 4, Detachable coupled to the lower portion of the storage unit 300, provided with an extraction stopper 310 is formed with an outlet 312 in the center to enter the syringe 10 Blood separation and concentration device for platelet-rich growth factor extraction, characterized in that. The blood separation and concentration device for platelet-rich growth factor extraction, characterized in that the support piece 116 is formed on the lower portion of the lower body 100 to support the extraction stopper 310 from the lower side. According to claim 5, Platelet-rich growth factor, characterized in that the amount of platelet enriched extracted through the outlet 312 is variable according to the height of the extraction plug 310 coupled to the lower portion of the storage unit 300 Blood separation and concentration device for extraction.

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