KR20200021033A - Centrifugal separation case assembly - Google Patents

Abstract

A centrifugal container assembly of the present invention modifies a structure to minimize an amount of a buffy coat which remains in a syringe to be lost when PRP having high viscosity and high concentration and the buffy coat are extracted by the syringe and injected into a human body; prevents component parts from being escaped by centrifugal force during centrifugation of blood; and prevents PPP in a layer-separated blood from being introduced into a storage part (35) for storing the buffy coat.

Description

Centrifuge Container Assembly {CENTRIFUGAL SEPARATION CASE ASSEMBLY}

The present invention relates to a centrifuge container assembly, and in particular, a high viscosity PRP (Platter Rich Plasma) (hereinafter referred to as 'PRP') and a buffy coat, which are centrifuged at high concentration, are extracted by a human body. The structure is improved to minimize the amount of buffy coat remaining in the syringe when it is added to the syringe, and prevents the falling of the container constituting the centrifuge vessel during the centrifugation of blood, and has a low viscosity in the layered blood. Centrifugation to improve the mixing of PRP and Buffy Coat to prevent PPP from entering the container where Buffy Coat will be stored when separating and collecting Platelet Poor Plasma (PPP) (hereinafter referred to as 'PPP') A container assembly.

Blood consists of red blood cells (RBC) (hereinafter referred to as 'RBC'), white blood cells (WBC: referred to as 'WBC'), platelets (hereinafter referred to as 'PLT') and plasma.

PRP (Platelet Concentrated Plasma) is a blood obtained by centrifuging its blood to remove RBCs and separating a platelet and a buffy coat layer rich in growth factors (Growth factor). Platelets and leukocytes are highly concentrated.

The PRP is located in the lower part of the plasma and contains growth factors such as cytokines, PDGF, TGF-BETA1, and VEGP, which are particularly effective in treating skin diseases and wound healing. ought.

In addition, a platelet-free plasma called PPP (platelet lean plasma) is used for autologous fillers or cosmetics derived from autologous plasma.

Centrifugation of blood at 3000 to 4000 RPM for 3 to 5 minutes, as shown in Figure 1, from the top of the vessel, PPP (A), PRP (P), buffy coat (B) (PLT and white blood cells (WBC)), RBC (C) are separated in order, high concentration PRP (P) is only a small amount of collected blood (about 1% of the collected blood), high viscosity and buffy coat (B) bordering the RBC is difficult to collect .

Usually, PLP and high concentration buffy coat (B) are obtained and injected into a patient by using a syringe from a container where blood is centrifuged.

However, PLP maintains a separate layer of PRP and buffy coat (B) even after being transferred from the centrifuge container to the syringe, as shown in FIG. 2A, and after injection into the patient, Since the viscosity is high, it remains attached to the inner surface of the syringe needle and the like, so that the loss is large.

2b is a state in which the PRP is cut into the syringe after the injection, and then collected with a cotton swab, smeared on a slide, and stained with a light stain, and observed under a microscope, showing the shape of the PLT and the WBC attached to the inside of the syringe.

Therefore, shake the syringe before injection to the patient to mix the relatively low viscosity PRP and the highly concentrated buffy coat (B) to lower the overall viscosity of the buffy coat (B) buffy coat (B) remaining in the syringe Induces loss prevention, but since the inside of the syringe is filled with PRP and buffy coat, there is no free space, which makes it difficult to mix PRP and buffy coat (B).

For this purpose, as shown in Figure 3, the two syringes are connected to each other and the PRP and buffy coat (B) is mixed by flowing back and forth, but the work is cumbersome, a small amount of buffy in the butted syringe The coat B is buried and residual loss occurs.

In addition, when the operator directly withdraws the PLP and buffy coat (B) centrifuged as described above from the centrifuge container with a syringe, the RBC contacted with the buffy coat (B) is sucked together and taken out Skill level is required.

4 to 6 are disclosed in Korean Patent No. 1432,393, which shows a centrifugal container having a storage unit for easily separating and containing the buffy coat.

It is formed in the lower receiving space 102 for receiving blood, the inner diameter is narrower than the lower portion at the upper portion of the receiving space 102 is formed with a passage portion 105 is opened at the top and the separation space portion ( The body 100 having the 104 formed thereon and the lifting space for flowing into the separation space 104 side by pushing the separation layer of blood arranged to be lifted below the receiving space 102 and separated by centrifugation upwards. The stopper 200 and the coupling part 310 is detachably coupled to the upper portion of the body 100 and coupled to communicate with the upper and inner portions of the passage part 105 at the center bottom when the body 100 is coupled with the body 100. It is formed and formed to extend to the lower side of one side of the coupling portion 310, the upper cap having a storage unit 320 is introduced into the concentration platelet (P) in the separation layer of blood when the lifting stopper 200 is raised ( 300).

Separation space 104 has a structure partitioned through the receiving portion 102 and the passage portion 105 on the upper side of the body 100, a portion of the separation layer of blood separated by the centrifugal separation of the blood is elevated When the stopper 200 is raised, it is configured to be received and received through the passage part 105.

When the centrifugal separation is completed, as shown in FIG. 5, the upper cap 300 is rotated and raised to move the coupling portion 310 and the passage portion 105 upwardly to be spaced apart from each other, and the lifting stopper 200 is raised. PPP (W) of the uppermost layer is separated into the separation space 104.

Subsequently, as shown in FIG. 6, the lowering operation of the upper cap 300 is performed again so that the coupling part 310 is fitted to the end of the passage part 105, and the buffy coat is operated by the lifting operation of the lifting stopper 200. P) is moved to the storage unit 320, and the buffy coat P is taken out by a syringe.

However, the above configuration has the following problems.

First, when the container is rotated at a high speed in the centrifuge, as shown in FIG. 7, the coupling part 310 coupled to the inner side of the upper cap 300 is separated to fall down the passage part 105. have.

In this case, there is a problem of contaminating the blood and the hassle to recombine the coupling portion 310 by opening the upper cap 300.

Second, the buffy coat (P), which is a very small amount of centrifuged blood, forms a boundary with the red blood cells (R). When the buried coat (P) is separated into the storage unit 320 by the elevation of the lift stopper 200, the red blood cells are separated. In order to minimize the loss of buffy coat (P) located at the boundary with (R), the passage portion 105 is formed thin and long to reduce the cross-sectional area of the boundary with the red blood cells (R) to minimize the loss of buffy coat (P). Doing.

That is, when the buffy coat (P) is injected into the human body when the red blood cells (R) is mixed, the buffy coat (P) at the interface with the red blood cells (R) is inevitably lost.

Since the passage portion 105 has to be formed to be thin and long for the same reason, the separation space portion 104 is formed by raising the lifting stopper 200 as shown in FIG. 5 with the PPP (W) separated on the top of the separated blood. In the separation, the lifting pressure 200 acts on the elongated passage portion 105 even with a small lifting force of the lifting stopper 200 so that the PPP (W) is discharged from the end portion of the passage portion 105, thereby storing the storage portion ( There is a problem to be introduced into 320).

That is, the patent has a buffy coat (P) when separating the PPP (W) to the separate storage unit 104 because the upper end of the elongated passage portion 105 and the upper end of the storage unit 320 are adjacent to each other. There is a problem that the PPP (W) is introduced into the storage unit 320 to be stored.

Korean patent registration

The present invention was created in order to solve the above problems, by forming a closed portion between the upper cap and the intermediate cap to store the PRP and buffy coats are stored, the high viscosity PRP and relatively low viscosity buffy coat It is possible to mix in the storage unit, to provide a centrifugal container assembly to reduce the loss of the PRP and buffy coat remaining in the syringe after the injection of the PRP and buffy coat to the human body by lowering the viscosity later injection into the human body Its purpose is to.

Another object of the present invention is to provide a centrifugal container assembly which prevents PPP from flowing into the storage part when separating the PPP into the separating space part, and also minimizes the loss of the buffy coat into the separating space part.

It is another object of the present invention to provide a centrifugal container assembly which can minimize the incorporation of red blood cells in contact with the buffy coat into the reservoir when the buffy coat is extracted into the reservoir.

The present invention to achieve the above object in the centrifuge vessel assembly for centrifuging the blood with red blood cells (RBC), PRP and Buffy coat (Puffy coat), PPP,

The lower portion is opened and a receiving space portion is formed therein, a PPP separated by centrifugal separation of the blood is received at the upper portion, and a separating space portion formed with a first male screw is formed on the outer circumferential surface of the upper portion, wherein the receiving space portion and the separating space are formed. A cylindrical container body configured to define a first passage pipe through which the passage is narrowed from the lower portion to the upper portion and through which the separated blood portion of the receiving space passes;

A lift stopper coupled to a lower portion of the accommodation space and configured to lift the blood separated by the centrifugation to be discharged to the first passage pipe;

Rising means for raising the lifting stopper;

A lower coupling portion screwed to an upper end of the container body and having a first female screw formed thereon, an upper coupling portion extending upward from the lower coupling portion and having a second male screw formed on an outer circumferential surface thereof, and a bottom formed at an inner lower end of the lower coupling portion; A second passage tube formed through the plate, the bottom plate and detachably connected to the first passage tube, and the PRP and buffy formed inside the upper coupling portion and passing through the second passage tube. An intermediate cap having a storage unit for storing a coat and connecting or separating the first passage pipe and the second passage pipe;

It is characterized in that it comprises a; upper cap is closed and screwed with the upper coupling portion and having a handle portion formed with a second female screw on the inner peripheral surface to open and close the upper end of the second passage pipe.

In addition, the lifting means is a cylindrical handle is formed on the outer peripheral surface of the lower end of the container body, a cylindrical handle formed with a third female screw screwed to the third male screw of the container body on the inner side, and is disposed inside the handle The upper end is provided with a lifting rod having a push rod coupled with the lifting stopper,

By the rotation of the lifting rod is characterized in that the lifting stopper coupled to the push rod is lifted.

In the assembly of the present invention, a circumferential jaw is formed on the inner surfaces of the upper end portions of the first passage pipe and the second passage pipe,

Characterized in that the cross-sectional area of the interface between the buffy coat and the RBC is reduced.

In addition, in the assembly of the present invention, the first passage pipe and the second passage pipe is formed to have a smaller diameter toward the top,

It has an elastic stopper coupled to the upper surface of the upper cap,

The elastic stopper is characterized in that the injection cap enters the supply cap portion for supplying blood to the receiving space, and the discharge cap portion capable of entering the needle to extract the PRP and buffy coat stored in the storage to the outside is formed. do.

In addition, the bottom plate is characterized in that it is formed concave.

First, by allowing the storage 35 formed between the upper cap 40 and the intermediate cap 30 to be closed, the high viscosity PRP and the relatively low viscosity buffy coat P are mixed in the storage 35. It is possible to reduce the viscosity to reduce the amount of PRP and buffy coat (P) remaining in the syringe after the injection to the human body by extracting the PRP and buffy coat (P) with a syringe afterwards.

Second, even if the container assembly of the embodiment of the present invention is rotated at a high speed, the storage unit 35 is stored by the combination of the upper cap 40 and the intermediate cap 30, the PRP and buffy coat (P), the prior art In the container 40 is separated from the upper cap 30 to solve the problem of falling to the separation space portion 12 side.

Third, since the upper end of the second passage pipe 34 is closed by the upper cap 40 when separating the PPP (P) into the separation space portion 12, PPP during the sudden ascending operation of the elevating stopper 20 Even if (P) is discharged from the end of the first passage pipe 13, it is possible to prevent the PPP (P) from flowing into the storage unit 35.

Fourth, since the elevating rod 60 is elevated by the rotation of the operator, it is possible to prevent the sudden rise of the elevating stopper 20 and fine adjustment is possible, as well as the PPP (W) and buffy coat ( Since the boundary surface of P) is reduced, the buffy coat P is introduced into the separation space 12 to minimize the loss.

Fifth, when the buffy coat P is extracted to the storage unit 35 when the lifting stopper 20 is raised, the cross-sectional area of the buried coat P and the red blood cells R is reduced by the circumferential jaw 34a. Red blood cells (R) in contact with (P) can be minimized to be incorporated into the storage (35).

1 is a schematic diagram showing a state in which the PRP and buffy coats are extracted by a syringe from a conventional centrifuge container,
Figure 2a is a cross-sectional view of the PRP and buffy coat extracted in the syringe,
Figure 2b is a magnified photograph observed after the injection of PRP, cut the inside of the syringe and collected with a cotton swab and smeared on a slide and stained with Light Stain,
Figure 3 is a schematic diagram showing a state of mixing the PRP and buffy coat extracted in the conventional syringe,
4 to 6 is an operating state diagram showing an operating state of extracting PRP and buffy coat from another conventional centrifugal vessel,
Figure 7 is a schematic diagram showing a problem during the centrifugation of the centrifuge vessel of Figure 4,
8 is an exploded perspective view showing the centrifugal container assembly of the embodiment of the present invention;
9 is an exploded cross-sectional view of FIG. 8;
10 is a cross-sectional view illustrating a state in which blood is collected and centrifuged into a centrifugal container assembly according to an embodiment of the present invention;
11 is a view for explaining a state of opening the upper end of the first passage pipe and separating the PPP,
12 is a view showing a process of opening the upper end of the second passage pipe and extracting the PRP and buffy coat.

The centrifugal container assembly of the embodiment of the present invention enables mixing of the PRP and buffy coat, so that PRP and buffy coat of centrifuged concentrations are taken out of the syringe and left in the syringe when dropped into the human body, and The structure is improved to minimize the buffy coat amount.

In addition, to solve the problem that the container constituting the container assembly is dropped during the process of centrifuging the blood as in the prior art, and that the PPP is introduced into the storage 35 to store the buffy coat of the separated blood It has an improved structure to prevent it.

8 to 10, the centrifuge container assembly of the embodiment of the present invention for centrifuging blood with red blood cells (RBC) (R), PRP and Buffy coat (P), and PPP (W), The container body 10, the intermediate cap 30 is screwed to the upper end of the container body 10, the upper cap 30 is coupled to the upper end of the intermediate cap 30, and the container body 10 An elevating stopper 20 coupled to the lower inner side so as to be elevated, and an elevating means for raising the elevating medium 20.

The container body 10 is a cylindrical shape, the lower portion is opened and the receiving space portion 11 is formed to accommodate the blood, the PPP (W) separated by the centrifugation of the blood is accommodated in the upper and the outer peripheral surface of the upper A separation space portion 12 having one male thread 14 is formed, which divides the accommodation space portion 11 and the separation space portion 12, and is formed to narrow a passage from a lower portion to an upper portion, and the accommodation space portion 11. The first passage pipe 13 through which the separated blood of the passage is formed.

The elevating cap 20 is coupled to the lower inner side of the accommodation space 11 by a lifting means, and lifts the blood separated by the centrifugation to discharge the blood to the first passage pipe 13. .

The intermediate cap 30 is screwed to the upper end of the container body 10 and the lower coupling portion 31, the first female screw 31a is formed, and extends upward from the lower coupling portion 31 and the outer peripheral surface An upper coupling portion 32 having two male threads 32a, a bottom plate 33 formed on the inner bottom of the lower coupling portion 31, and a bottom portion 33 formed through the bottom plate 33 and having a lower end portion formed therein. The second passage tube 34 detachably connected to the first passage tube 13 and the PRP and buffy coat formed inside the upper coupling part 32 and passing through the second passage tube 34 are stored. It is provided with a storage unit 35.

The intermediate cap 30 connects or separates the first passage tube 13 and the second passage tube 34 according to the screw engagement state with the container body 10.

The volume of the reservoir 35 is formed relatively larger than the volume of the centrifuged PRP and buffy coat, so that the PRP and buffy coat can be mixed in the reservoir 35 when shaking the container body 10. .

The upper cap 40 is closed by the upper surface and screwed with the upper coupling portion 32 of the intermediate cap 30, the second passage having a handle portion 41 formed with a second female screw 41a on the inner peripheral surface Opening and closing the upper end of the tube (34).

In addition, the upper cap 40 is coupled to the elastic stopper 50, the elastic stopper 50 is formed of a thin rubber material so as to be reclosable and the needle is entered into the blood receiving space 11 It is provided with a supply cap 51 for supplying, and an outflow cap 52 capable of entering the needle to extract the PRP and buffy coat stored in the storage 35 to the outside.

The elevating means is provided with a lifting rod 60 which is formed on the outer peripheral surface of the lower end of the container body 10 and has a third male screw 11a screwed to the third male screw 11a.

The elevating rod 60 has a cylindrical handle 61 formed with a third female screw 61a screwed to the third male screw 11a of the container body 10 on the inner surface thereof, and on the inner side of the handle 81. It is provided with a push rod 62 is disposed and the upper end is coupled to the elevating stopper 20.

By the rotation of the elevating rod 60, the elevating stopper 20 coupled with the push rod 62 is elevated.

Meanwhile, in FIG. 8, reference numeral “70” is a lower cap, which is screwed to the lower end of the container body 10 to prevent the lifting stopper 20 from being separated during storage or transportation of the container assembly, and the lifting rod 60. When using) remove the lower cap 70 is used.

Meanwhile, circumferential jaws 13a and 34a are formed around the inner circumferential surfaces of the upper ends of the first passage pipe 13 and the second passage pipe 34. In this case, the cross-sectional area of the interface between the PPP (W) and the buffy coat (P) is reduced by the circumferential jaw 13a, so that the PPP (W) is extracted to the separation space 12 when the lifting stopper 20 is raised. When the buffy coat (P) in contact with the PPP (W) is to be minimized the amount of discarded into the separation space (12).

In addition, the interface cross-sectional area between the buffy coat (P) and the red blood cells (R) is reduced by the circumferential jaw (34a), so that buffy coat (P) to the storage unit 35 when the lifting stopper 20 is raised When extracted, red blood cells (R) contacting the buffy coat (P) can be minimized to be incorporated into the storage (35).

In addition, the bottom plate 33 of the storage unit 35 is concave to form a high viscosity and a small amount of PRP and buffy coat (P) as possible without remaining in the storage unit 35 by the needle 100 To be extracted.

Blood separation operation using the centrifugal container assembly of the embodiment of the present invention having the configuration as described above is as follows.

[Blood supply and centrifugation]

Referring to FIG. 10, after the needle 100 of the syringe in which the blood is stored passes through the supply cap 51, the receiving space portion of the container body 10 through the first and second passage tubes 13 and 34. 11) Blood is supplied into the blood, and the blood injected into the receiving space 11 is centrifuged using a centrifuge.

At this time, the first and second passage pipes 13 and 34 are connected, and the upper end of the second passage pipe 34 is closed by the upper cap 40.

When the container assembly is rotated at a high speed of 3 to 5 minutes at 3,000 to 4,000 RPM, the blood is centrifuged to separate red blood cells (RBC; R), PRP and buffy coat (P) and PPP (W) from the lower portion of the receiving space 11. Layered.

Even if the container assembly of the embodiment of the present invention is rotated at a high speed, as shown in Figure 7 solves the problem that the container 40 in the prior art is separated from the upper cap 30 to fall to the separation space portion 12 side.

[PPP separation water]

Referring to FIG. 11, after the layers are separated into red blood cells (RBC; R), PRP and buffy coat (P), and PPP (W), the intermediate cap 30 is rotated to form an upper end of the first passage pipe 13. The lower end portion of the second passage pipe 34 is spaced apart so that the accommodation space 11 and the separation space 12 communicate with each other.

Subsequently, when the lifting stopper 20 is rotated upward by rotating the lifting bar 60, the lifting stopper 20 is moved up inside the accommodation space 11 while separating layers of blood stored in the accommodation space 11. Among them, the PPP (W) layer located at the uppermost portion is pushed into the separation space 12 through the first passage pipe 13.

At this time, since the upper end of the second passage 34 is closed by the upper cap 40, even when PPP (P) is discharged from the end of the first passage 13 when the lifting stopper 20 suddenly moves up. PPP (P) can be prevented from flowing into the storage unit 35.

In addition, since the elevating rod 60 is elevated by the rotation of the operator, it is possible to prevent the sudden rise of the elevating stopper 20 and fine adjustment is possible, as well as the PPP (W) and the buffy coat by the circumferential jaw 13a. Since the boundary surface of (P) is reduced, the buffy coat (P) is introduced into the separation space portion 12 to minimize the loss.

[PRP and buffy coat separation]

Referring to FIG. 12, after the PPP (W) of the uppermost layer of the separated blood is separated into the separating space part 12, the intermediate cap 30 is rotated to rotate the first passage tube 13 and the second passage tube. 34 to be connected so that the receiving space 11 and the separation space 12 is blocked.

Subsequently, the upper cap 40 is rotated so that the upper end portion of the second passage tube 34 and the bottom surface of the upper cap 40 are spaced apart from each other to communicate with the storage 35.

Subsequently, when the lifting stopper 20 is elevated by rotating the elevating rod 60, PRP and buffy coat P are introduced into the storage 35 through the upper end of the second passage 34. ~ 30cc is stored.

In addition, the interface cross-sectional area between the buffy coat (P) and the red blood cells (R) is reduced by the circumferential jaw (34a), so that buffy coat (P) to the storage unit 35 when the lifting stopper 20 is raised When extracted, red blood cells (R) contacting the buffy coat (P) can be minimized to be incorporated into the storage (35).

That is, the buffy coat (P) separated on the upper side of the RBC (R) is a small amount when the RBC (R) is mixed when injected into the human body causes pain, so by forming the circumferential jaw (34a) the upper surface of the RBC (R) It is possible to minimize the amount of buffy coat (P) remaining in the loss.

[PRP and Buffy Coat Mixing]

As shown in FIG. 12, after the PRP and buffy coat P are introduced into the storage unit 35, the container body 10 is shaken to mix the PRP and buffy coat P in the storage unit 35.

By mixing the high viscosity buffy coat and the relatively low viscosity PRP, the viscosity is lowered to extract the buffy coat (P) with a syringe afterwards to reduce the amount of buffy coat (P) remaining in the syringe after injection into the human body.

[PRP and Buffy Coat Extraction]

Referring to FIG. 12, the needle 100 of the syringe passes through the outlet cap 52 of the upper cap 40 to enter the storage 35, and then the mixed PRP and buffy coat P are extracted. do.

At this time, the bottom of the storage unit 35 is formed to be concave, thereby reducing the amount of PRP and buffy coat (P) remaining in the storage unit 35.

The centrifugal container assembly of the present invention as described above minimizes the loss of centrifuged PRP and buffy coat remaining in the syringe and the centrifuge container, and the container constituting the container assembly is dropped during the centrifugation of blood. When the PPP (platelet rich plasma) is separated and collected in the separated blood, the PPP may be prevented from being introduced into the storage 35 to store the buffy coat.

10.Container Body 11 ... Accommodation Space
12 ... Separation space 13 ... First passage pipe
20 ... Elevation stopper 30 ... Medium cap
34.2nd passage 35 35.Storage part
40 ... upper cap 60 ... lift bar

Claims (5)

In the centrifuge vessel assembly for centrifuging the blood with red blood cells (RBC), PRP and Buffy coat (PPP), and PPP,
Separation space portion 12 having a lower portion is opened and a receiving space portion 11 is received, the PPP is separated by centrifugal separation of the blood at the upper portion and the first male screw 14 is formed on the outer peripheral surface of the upper portion Is formed, the first passage pipe is formed so as to partition the receiving space 11 and the separation space 12 and the passage from the bottom to the narrow and the separated blood of the receiving space (11) ( 13 and a cylindrical container body 10 formed;
A lift stopper 20 coupled to the lower portion of the accommodation space 11 so as to elevate the blood separated by the centrifugation to be discharged to the first passage pipe 13;
Elevating means for raising the elevating stopper 20;
The lower coupling portion 31 is screwed to the upper end of the container body 10 and the first female screw 31a is formed, and the second male screw 32a is formed on the outer circumferential surface and extends upward from the lower coupling portion 31. The upper coupling portion 32, the bottom plate 33 formed on the inner lower end of the lower coupling portion 31, and formed through the bottom plate 33, the lower end of the first passage pipe 13 and A second passage pipe 34 detachably connected to the storage unit 35 is formed inside the upper coupling portion 32 and stores the PRP and buffy coat passing through the second passage pipe 34. And an intermediate cap (30) for connecting or separating the first passage pipe (13) and the second passage pipe (34);
An upper cap 40 having an upper surface closed and screwed with the upper coupling portion 32 and having a handle portion 41 having a second female screw 41a formed on an inner circumferential surface thereof to open and close an upper end portion of the second passage pipe 34. Centrifugal container assembly, characterized in that provided with. The method according to claim 1, wherein the lifting means has a third male screw (11a) is formed on the outer peripheral surface of the lower end of the container body 10,
A cylindrical handle 61 having a third female screw 61a which is screwed to the third male screw 11a of the container body 10 on the inner surface thereof, and an upper end of which is disposed inside the handle 81 and having an upper end cap. With a lifting rod 60 having a push rod 62 coupled with 20,
Centrifugal container assembly, characterized in that the lifting stopper 20 is coupled to the push rod 62 by the rotation of the lifting bar (60). The circumferential jaws 13a and 34a are formed on inner surfaces of the upper end portions of the first passage pipe 13 and the second passage pipe 34, respectively.
Centrifuge vessel assembly, characterized in that the cross-sectional area of the interface between the buffy coat and the RBC is reduced. The method of claim 1, wherein the first passage pipe 13 and the second passage pipe 34 is formed so that the diameter becomes smaller toward the top,
It has an elastic stopper 50 coupled to the upper surface of the upper cap 40,
The elastic stopper 50 is a needle to enter the injection cap portion 51 for supplying blood to the receiving space 11 and the injection to extract the PRP and buffy coat stored in the storage 35 to the outside Centrifugal container assembly, characterized in that the outlet cap 52 is formed that can enter the needle. The centrifugal container assembly according to any one of claims 1 to 4, wherein the bottom plate (33) is concave.

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