KR102119835B1 - Centrifuge device for separating blood components - Google Patents

Abstract

The present invention relates to a device for centrifuging blood components. The present invention, the upper container including an extraction tube having a smaller diameter than other parts; An upper closure portion coupled to close the upper opening portion of the upper container; A lower container coupled to a lower end of the upper container; And a lower closure portion coupled to close the lower opening portion of the lower container, wherein the upper closing portion is provided with an inner partition plate partitioning the upper container inside the upper closing portion, and the lower closing portion includes: A liftable piston member for adjusting the inner volume of the lower container is provided, and the extraction tube closing member provided at the top of the lower container closes the lower part of the extraction tube by rotating the lower container with respect to the upper container. Provided is a blood component centrifugation device.

Description

Blood component centrifugation device {CENTRIFUGE DEVICE FOR SEPARATING BLOOD COMPONENTS}

The present invention relates to a device for centrifuging blood components. More specifically, the present invention relates to a device for centrifuging blood components that facilitates extraction of desired components by centrifuging components contained in blood.

Mesenchymal stem cells have received much attention for purposes such as regenerative medicine, tissue engineering and gene therapy. This is because mesenchymal stem cells have the ability to differentiate into various cells with continuous self-renewal ability. These mesenchymal stem cells can be obtained from many tissues such as bone marrow, umbilical cord blood, adipose tissue, placenta, and amniotic fluid.

On the other hand, the plasma contained in the blood (plasma) is a neutral pale yellow liquid component separated from the blood cells from the blood, and platelet-rich plasma (PRP) obtained by centrifuging the plasma improves skin wrinkles, skin or ligaments, or Used for the purpose of strengthening and regenerating muscles. In addition to the term PRP in centrifugal extraction of blood components, the term buffy coat including white blood cells formed as a white layer between the plasma layer and the red blood cell layer is also used. When stem cells are included in the blood, the stem cells can be separated by being included in a buffy coat.

To extract useful components from the blood, the blood is centrifuged, and in order to obtain useful components by concentration, an appropriate centrifugation device needs to be used.

For example, Republic of Korea Patent Registration No. 10-1049201 (2011.07.07. registration) discloses a device for extracting a centrifuged buffy coat layer by inserting a syringe through a syringe guide.

The device for extracting a desired substance by centrifuging blood prevents contamination in the process of extracting a specific component after injecting blood and centrifuging the blood, and after the desired component is centrifuged, the component is separated from other components. It is necessary to prevent remixing, to easily extract the centrifuged components from the outside, and to ensure user convenience.

Various types of centrifugal separation devices have been proposed in the prior art, but they do not meet the above-described comprehensive requirements.

Accordingly, an object of the present invention is to provide an improved blood component centrifugation device that satisfies the above requirements.

The present invention, the upper container including an extraction tube having a smaller diameter than other parts; An upper closure portion coupled to close the upper opening portion of the upper container; A lower container coupled to a lower end of the upper container; And a lower closure portion coupled to close the lower opening portion of the lower container, wherein the upper closing portion is provided with an inner partition plate partitioning the upper container inside the upper closing portion, and the lower closing portion includes: A liftable piston member for adjusting the inner volume of the lower container is provided, and the extraction tube closing member provided at the top of the lower container closes the lower part of the extraction tube by rotating the lower container with respect to the upper container. Provided is a blood component centrifugation device.

In one embodiment, the upper container is formed on the upper portion of the extraction pipe and has an upper body having a larger diameter than the extraction pipe and a lower body formed on the lower portion of the extraction pipe and having a larger diameter than the extraction pipe, The lower container may be screwed to the lower body.

In addition, the upper body, the first body, the first body and the second body connected through the first shaft diameter and smaller in diameter than the first body, the second body and the second shaft diameter connected through And a third body having a smaller diameter than the second body, and may include a third shaft diameter connecting the third body and the extraction tube.

In addition, the inner partition plate may be positioned in contact with the inner circumferential surface of the second body, and may be closed by dividing the upper body up and down in contact with the inner circumferential surface of the second shaft or the third body.

In addition, the upper closing portion, the upper cap coupled to the upper cap engaging projection formed on the outer circumferential surface of the first body and the upper cap for closing the top of the upper container, and is provided on the bottom surface of the upper cap and has a thread on the outer circumferential surface It includes a formed support rod, and the inner partition plate can be elevated by being screwed to the support rod.

In addition, a check valve may be provided on the upper surface of the upper cap to allow air flow from inside to outside of the upper container.

In addition, the support rod may be formed with an internal through channel formed through the top and bottom, and the inside through channel may be provided with a blocking member including a filter for filtering air.

In addition, the inner partition plate, the outer peripheral portion in contact with the inner circumferential surface of the second body, the support rod coupling portion screwed to the support rod, and a border formed to penetrate up and down at a position greater than a predetermined radius from the center of rotation of the inner partition plate It may be formed including a through portion.

In addition, the lower body, the lower container upper insertion portion connected to the lower end of the extraction tube, the lower container coupling portion connected to the lower end of the lower container upper insertion portion is formed on the inner circumferential surface so that the upper end of the lower container is screwed It can contain.

In one embodiment, the lower container includes a lower container body and a lower container upper body provided on an upper portion of the lower container body, and the extraction pipe closing member opens an upper end of the upper container of the lower container through a connecting rib. It can be coupled to the secondary side.

In addition, a through portion may be formed between the connecting ribs.

In addition, a sealing member for sealing a contact surface with the upper container may be provided on an outer peripheral surface of the upper body of the lower container.

Meanwhile, a stopper insertion tube may be formed in the extraction tube, and a stopper capable of inserting a needle of a syringe may be coupled to the stopper insertion tube.

In addition, the lower closure portion, the lower cap coupled to the lower cap engaging projection formed on the outer peripheral surface of the lower container and a lifting screw rod provided in the center of the upper surface of the lower cap, the piston member is The lifting screw rod may rotate and elevate according to the rotation of the lower cap.

According to the present invention, blood is injected to centrifuge the blood, and external contamination is prevented in the process of extracting a specific component that is centrifuged.

In addition, according to the present invention, after centrifuging the blood, the specific component to be extracted can be adjusted to be located in the recess of the upper container, and the lower container is rotated to close the bottom of the recess to extract the component and lower container. It has the effect of preventing the mixing of the components contained in. This process can be performed by the user rotating the lower closure portion at the bottom of the lower container, and rotating the lower container itself, thereby improving usability.

In addition, according to the present invention, as the upper cap of the upper closure part rotates, the inner partition plate divides the inner space of the upper container up and down, and the components located at the lower portion of the inner partition plate together with a specific component located in the extraction tube There is an advantage that can be extracted and used in the procedure.

In addition, according to the present invention, when the lower end of the extraction tube is closed and the inside of the upper container is partitioned into the inner partition plate, when extracting the internal components using the needle of the syringe, external air can enter to facilitate extraction. It has the advantage of making it possible.

1 is a perspective view of a blood component centrifugation device according to a preferred embodiment of the present invention.
Figure 2 is a cross-sectional view of the blood component centrifugation device according to a preferred embodiment of the present invention (section AA' of Figure 1).
3 is an exploded perspective view of a blood component centrifugation device according to a preferred embodiment of the present invention.
4 is a perspective view (a) and a cross-sectional view (b, BB' cross section of FIG. 4(a)) of the upper container of the blood component centrifugation device according to the preferred embodiment of the present invention.
5 is a perspective view (a) and a cross-sectional view of the lower container of the blood component centrifugation device according to a preferred embodiment of the present invention (b, CC' cross section of FIG. 5 (a)).
6 is an exploded perspective view of an upper closure of a blood component centrifugation device according to a preferred embodiment of the present invention.
7 is a perspective view (a) and a cross-sectional view of the lower closure portion of the blood component centrifugation device according to a preferred embodiment of the present invention (b, DD' cross section of FIG. 7(a)).
8 is a view showing a process of injecting blood into a blood component centrifugation device according to a preferred embodiment of the present invention.
9 is a view showing a step-by-step operation of the blood component centrifugation device according to a preferred embodiment of the present invention.
10 is a view showing a process of extracting a specific component separated from the blood component centrifugation device according to a preferred embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. First, when adding reference numerals to the components of each drawing, it should be noted that the same components have the same reference numerals as possible even though they are displayed on different drawings. In addition, in describing the present invention, when it is determined that detailed descriptions of related well-known structures or functions may obscure the subject matter of the present invention, detailed descriptions thereof will be omitted. In addition, although preferred embodiments of the present invention will be described below, the technical spirit of the present invention is not limited to or limited thereto, and can be variously implemented by a person skilled in the art.

1 is a perspective view of a blood component centrifugation device according to a preferred embodiment of the present invention, Figure 2 is a cross-sectional view of a blood component centrifugal device according to a preferred embodiment of the present invention (cross section AA' in Fig. 1), Figure 3 Is an exploded perspective view of a blood component centrifugation device according to a preferred embodiment of the present invention.

In addition, Figure 4 is a perspective view (a) and a cross-sectional view (b) of the upper container of the blood component centrifugation device according to a preferred embodiment of the present invention, Figure 5 is a blood component centrifugation device according to a preferred embodiment of the present invention It is a perspective view (a) and a sectional view (b) of the lower container. Figure 6 is an exploded perspective view of the upper closure of the blood component centrifugation device according to a preferred embodiment of the present invention, Figure 7 is a perspective view (a) and a cross-sectional view of the lower closure of the blood component centrifugation device according to a preferred embodiment of the present invention (b).

A blood component centrifugation device 10 according to a preferred embodiment of the present invention will be described with reference to FIGS. 1 to 7.

The blood component centrifugation device 10 according to a preferred embodiment of the present invention includes an upper container 100, an upper closure part 200 coupled to close the upper opening part of the upper container 100, and the upper container ( 100) a lower container 300 coupled to the lower end, and a lower closing part 400 coupled to close the lower opening of the lower container 300. The blood component centrifugation device 10 is preferably made of a material that is harmless to the human body, and can be made of a transparent plastic material.

The interior of the upper container 100 and the lower container 300 forms an empty receiving space. The upper closure part 200 is coupled to the upper opening part of the upper container 100 to close the upper part of the upper container 100, and the lower closure part 400 is coupled to the lower opening part of the lower container 300 to be lower. The lower end of the container 300 is closed.

Referring to FIG. 4, the upper container 100 is connected from the top through the first body 110, the first body 110 and the first shaft diameter part 114, and has a diameter (or width) greater than that of the first body 110. ) Is connected to the second body 120, the second body 120 and the second shaft diameter portion 122 is small, the third body 130, the diameter (or width) smaller than the second body 120, the second 3 through the body 130 and the third shaft diameter portion 132 and the diameter (or width) smaller than the third body 130, the extraction pipe 140, the extraction pipe 140 through the expansion portion 152 It is connected and includes a fourth body 150 having a diameter greater than that of the concave portion 140. The upper container 100 is composed of an open top and bottom. The upper container 100 may be integrally injection molded using a material such as plastic.

In one embodiment, the first body 110, the second body 120, the third body 130, the extraction tube 140, and the fourth body 150 may be provided in a cylindrical shape.

The upper cap coupling protrusion 112 is protruded on the outside of the first body 110. The upper cap coupling protrusion 112 prevents the upper closure part 200 from being separated when the upper closure part 200 is coupled to the upper container 100.

The first shaft diameter portion 114, the second shaft diameter portion 122, the third shaft diameter portion 132, and the enlarged diameter portion 152 may have a tapered shape or an arc shape or a stepped shape having a predetermined curvature.

The extraction pipe 140 is a portion having the narrowest diameter or width, and at least one stopper insertion pipe 142 is formed in the extraction pipe 140 and a stopper 144 is inserted into the stopper insertion pipe 142. . The stopper 144 is a portion where the needle of the syringe is inserted, and when the needle of the syringe is inserted, the outer circumferential surface of the syringe needle is pressed to maintain airtightness, and even when the syringe needle is removed, the portion pierced with a syringe needle is closed. The stopper 144 may be made of a material such as silicon.

The fourth body 150 includes a lower container top insertion part 154 connected to the enlarged diameter part 152 and a lower container coupling part 156 connected to the lower container top insertion part 154. The lower container top insertion portion 154 and the lower container coupling portion 156 may be formed in a cylindrical shape. In addition, a thread 158 may be formed on the inner circumferential surface of the lower container coupling portion 156.

Referring to FIG. 5, the lower container 300 includes a lower container body 310 and a lower container upper body 320 provided on top of the lower container body 310, and the lower container upper body 320 It includes an extraction pipe closing member 330 provided in the upper central portion of the upper body 320 of the lower container through the connecting rib (332). The lower portion of the lower container 300 is opened and the upper portion of the lower container 300 is partially closed by the extraction pipe closing member 330.

The lower container body 310 is provided in a substantially cylindrical shape. A thread 312 is formed on the upper outer circumferential surface of the lower container body 310 to allow screw coupling to the inside of the lower container coupling portion 156 of the upper container 100. A lower cap coupling protrusion 314 protruding to the outside is formed at a lower end of the lower container body 310 so that the lower closing portion 400 is coupled so as not to escape.

The lower container upper body 320 may be provided in a cylindrical shape slightly smaller in diameter than the lower container body 310. A sealing member insertion groove 322 is formed outside the upper body 320 of the lower container, and a sealing member 338 such as an O-ring shown in FIG. 3 is coupled to the sealing member insertion groove 322. Can be.

The extraction pipe closing member 330 is connected to the upper end of the upper body 320 of the lower container through the connecting rib 332, and a portion other than the connecting rib 332 is formed is formed with a through portion 334. Through the through portion 334, the inner space of the lower container 300 and the space above the lower container 300 may communicate with each other.

Referring to FIG. 2, a portion of the lower container body 310 of the lower container 300 and the lower container upper body 320 are coupled to the inner side of the fourth body 150 of the upper container 100. Specifically, the upper end of the lower container body 310 is screwed to the inner circumferential surface of the lower container coupling portion 156 of the fourth body 150. The lower container upper body 320 is movably coupled up and down along the inner circumferential surface of the lower container upper insertion part 154, and the sealing member 338 mounted on the lower container upper body 320 has a lower container upper insertion part ( 154) to maintain airtightness by adhering to the inner peripheral surface. In the state of FIG. 2, the extraction tube closing member 330 of the lower container 100 is not in close contact with the lower end of the extraction tube 140, and the inner space and lower part of the upper container 100 through the through portion 334 The inner space of the container 300 is connected.

The upper closure part 200 will be described with reference to FIGS. 2, 3, and 6. The upper closure part 200 is coupled to the top of the upper container 100 to close the upper opening part of the upper container 100. The upper closure part 200 is provided with a support rod 220 formed extending from the center of the inner surface of the upper cap 210 and the upper cap 210 to the lower end, and screwed to the outside of the support rod 220 It includes an inner partition plate 230.

The upper cap 210 includes a cap member 212 and a lower extension portion 216 formed vertically downward from the ends of the cap member 212 and a locking protrusion 218 formed to protrude in the inner circumferential direction from the lower extension portion 216. It includes. An uneven surface 217 is formed on the outer circumferential surface of the lower extension portion 216 to facilitate gripping of the user. When the upper cap 210 is coupled to the top of the upper container 100, the locking projection 218 of the upper cap 210 is caught by the engaging projection 112 of the upper container 100, so that the upper cap 210 is Deviation is prevented, and the upper cap 210 can rotate freely while being coupled to the top of the upper container 100. Meanwhile, the upper cap sealing member 250 illustrated in FIG. 3 is coupled to the inside of the bottom surface of the upper cap 210 to seal the upper cap 210 and the upper end of the upper container 100 at a contact surface. The upper cap sealing member 250 may be formed as an O-ring.

A valve member mounting portion 214 may be formed on the cap member 212, and the check valve 215 may be provided on the valve member mounting portion 214. The check valve 215 may be configured to allow gas flow from inside the container to the outside but not gas flow in the opposite direction. Accordingly, when blood or the like is supplied to the inside of the container, the air inside the container can be discharged to the outside through the check valve 215, but the outside air is introduced into the container through the check valve 215. It becomes impossible.

A through hole 211 is formed in the central portion of the cap member 212, and the a through blocking member 213 may be provided in the through hole 211. The blocking member 213 may be a check valve allowing air flow from outside to inside of the cap member 212. Alternatively, the blocking member 213 may be a filter that removes foreign substances from the air.

The support rod 220 is formed with a thread 222 on the outer circumferential surface, and the support rod 220 is formed with an internal through channel 226 penetrating the inside in the vertical direction. The internal through channel 226 is connected to a through hole 211 formed in the cap member 212. External air may be introduced into the inner space of the upper container 100 through the inner through channel 226, and when a blocking member blocking the through hole 211 includes a filter, it may flow through the inner through channel 226. Contaminants can be removed from the air. The support rod 220 may have a stepped 224 formed above the portion where the thread 222 is formed, and the raised height of the inner partition plate 230 may be limited by the stepped 224.

The inner partition plate 230 is screwed to the support rod 220. The inner partition plate 230 may be formed in a disc shape having a predetermined thickness with an outer circumferential portion 232. In addition, the inner partition plate 230 is provided with a support rod coupling portion 238 having a thread 240 screwed to the support rod 220 on an inner circumferential surface. When the support rod 220 rotates according to the rotation of the upper cap 210, the inner partition plate 230 rises or falls according to the rotational direction of the upper cap 210. On the other hand, the inner partition plate 230 is formed with a rim through portion 236 formed to penetrate the top and bottom of the inner partition plate 230 at a position greater than a predetermined radius from the center of rotation.

Referring to FIG. 2, the inner partition plate 230 may be coupled to the outer circumferential portion 232 in contact with the inner circumferential surface of the second body 120 of the inner container 100. In the state of FIG. 2, the spaces of the inner container 100 at the top and bottom of the inner partition plate 230 are communicated through the rim through portion 236. However, when the inner partition plate 230 descends and the bottom surface of the outer circumferential portion 232 of the inner partition plate 230 comes into contact with the second shaft diameter portion 122 formed on the upper end of the third body 130, the edge penetration portion 236 ) Is blocked by the second shaft diameter part 122. Accordingly, the inner space of the inner container 100 is separated up and down by the inner partition plate 230 and does not communicate with each other.

On the other hand, in forming the inner partition plate 230, it is also possible to form a reinforcement rib 234 to increase the thickness in order to improve the strength in the inner partition plate 230.

The lower closure part 400 will be described with reference to FIGS. 2, 3, and 7.

The lower closing part 400 includes a lower cap 410 on which the lifting screw rod 418 is formed, and a piston member 420 that is screwed to the lifting screw rod 418 to be elevated.

The lower cap 410 includes a lower cap member 412 and an elevating screw rod 418 provided on an upper end of the lower cap member 412 and having a thread 419 formed on an outer circumferential surface. The outer side of the lower cap member 412 is provided with a top extension portion 414 formed to extend to the top, and a locking protrusion 416 formed to protrude in the inner circumferential direction from the top extension portion 414 is formed. An uneven surface 417 is formed on the outer circumferential surface of the upper extension 414 to facilitate user gripping. When the lower cap 410 is coupled to the lower end of the lower container 300, the locking projection 416 of the lower cap 410 is caught by the lower cap engaging projection 314 of the lower container body 310, thereby lowering the lower cap ( The separation of 410) is prevented, and the lower cap 410 can freely rotate in a state coupled to the lower end of the lower container 300.

The piston member 420 includes a packing coupling part 422, a piston packing 424 coupled to an outer circumference of the packing coupling part 422, and a threaded rod provided in a vertical direction on one side of the packing coupling part 422. It includes a coupling portion 426. In the state in which the piston packing 424 is coupled to the packing coupling portion 422, the outer circumference of the piston packing 424 contacts the inner circumferential surface of the lower container body 310 of the lower container 300.

The lifting screw rod 418 provided on the lower cap 410 is screwed to the thread formed on the inner circumferential surface of the screw coupling portion 426. As the lower cap 410 rotates, the lifting screw rod 418 rotates, and accordingly, the piston member 420 is raised or lowered. On the other hand, the piston cap 428 is coupled to the screw coupling portion 426 to maintain airtightness. However, in the practice of the present invention, the piston cap 428 may not be necessary when the screw rod coupling portion 426 is formed in a closed state.

Next, a process of centrifuging blood and extracting a specific component using the blood component centrifugation apparatus 10 according to the present invention will be described.

8 is a view showing a process of injecting blood into a blood component centrifugation device according to a preferred embodiment of the present invention.

The lower container 300 having the lower closing part 400 coupled to the lower part is coupled to the upper container 100 having the upper closing part coupled to the upper part. The extraction tube closing member 330 of the lower container 300 does not close the extraction tube 140 of the upper container 100, and the upper container 100 and the lower container 300 are in communication with each other. In addition, the inner partition plate 230 of the upper closing portion 200 is positioned in contact with the second body 120, and the inner partition plate 230 is positioned without closing the top of the third body 130 do.

The needle 502 of the first syringe 500 is inserted into the stopper 144 while the blood 600 is accommodated in the first syringe 500, and the blood 600 is injected into the container.

9 is a view showing a step-by-step operation of the blood component centrifugation device according to a preferred embodiment of the present invention.

9A shows a state in which blood 600 is injected into the blood component centrifugation vessel 10. The blood component centrifugation vessel 10 is mounted on a centrifuge and centrifugation is performed.

9(b) shows a state in which blood components are centrifuged. Components contained in the blood are divided up and down by specific gravity to form a layer. For example, red blood cells (RBC) 610 are located in the lowermost layer, and buffy coat 620 above the red blood cells 610, platelet-rich plasma (PRP) 630, and platelet-rich plasma (Platelet-rich plasma, PRP), and Platelet-poor plasma (PPP) 630 may be sequentially arranged in layers. When the bone marrow is centrifuged, the buffy coat 620 may include stem cells included in the bone marrow.

9C shows a state in which the piston member 420 of the lower closure part 400 is raised. The lower cap 410 is rotated to raise the piston member 420 so that the buffy coat 620 is located on the extraction tube 140 side of the upper container 100. In this case, it may be possible to raise the piston member 420 after raising the extraction tube closing member 330 of the lower container 300 by rotating the lower container 300 relative to the upper container 100. .

9(d) shows a state in which the extraction pipe closing member 330 and the internal partition plate 230 are moved.

In the state where the buffy coat 620 is located on the side of the extraction pipe 140, the lower pipe 300 is rotated relative to the upper vessel 100 so that the extraction pipe closing member 330 closes the lower portion of the extraction pipe 140. . The lower portion of the extraction pipe 140 is closed by the extraction pipe closing member 330 contacting the lower inner circumferential surface of the extraction pipe 140 or the upper inner circumferential surface of the first expansion part 152 connected to the lower end of the extraction pipe 140. Can be.

Next, the upper cap 210 is rotated so that the inner partition plate 230 descends. Components of the lower portion of the inner partition plate 230 are internal partitions until the outer side of the inner partition plate 230 blocks the upper side of the second shaft diameter portion 122 or the inner partition plate 230 and the third body 130. It moves to the upper portion of the inner partition plate 230 through the rim through portion 236 formed in the plate 230. When the inner partition plate 230 further descends, the upper portion of the third body 130 is closed by the inner partition plate 230 and the movement of the components becomes impossible.

10 is a view showing a process of extracting a specific component separated from the blood component centrifugation device according to a preferred embodiment of the present invention.

In the same state as in (d) of FIG. 9, a specific component is extracted using the second syringe 700.

Referring to FIG. 10, the needle 710 of the second syringe 700 is inserted into the stopper 144 and the buffy coat 620 located in the extraction tube 140 is extracted. While extracting the buffy coat 620, the PRP 630 may also be partially extracted. When extracting the components with the second syringe 700, external air is introduced through the inner through channel 226 formed in the support rod 220 to enable component extraction using the second syringe 700.

According to the present invention, the space by the extraction pipe 140 and the third body 130 is isolated from other parts by using the extraction pipe closing member 330 and the internal partition plate 230, so that the buffy coat 620 and the PRP ( 630) can be easily extracted.

The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains may make various modifications, changes, and substitutions without departing from the essential characteristics of the present invention. will be. Therefore, the embodiments disclosed in the present invention and the accompanying drawings are not intended to limit the technical spirit of the present invention, but to explain the scope of the technical spirit of the present invention. . The scope of protection of the present invention should be interpreted by the claims below, and all technical spirits within the equivalent range should be interpreted as being included in the scope of the present invention.

10: blood component centrifugation device
100: upper container 110: first body
112: upper cap engaging projection 114: the first shaft diameter
120: second body 122: the second shaft portion
130: third body 132: the third shaft portion
140: extraction tube 142: stopper insertion tube
144: stopper 150: the fourth body
152: first expansion portion 154: lower container top insert
156: lower container coupling portion 158: thread
200: upper closure 210: upper cap
212: cap member 218: a locking projection
220: support rod 226: internal through channel
230: inner partition plate 232: outer peripheral part
234: Reinforced rib 236: Border penetration
238: support rod coupling portion 250: upper cap sealing member
300: lower container 310: lower container body
314: lower cap engaging projection 320: lower container upper body
330: extraction pipe closing member 332: connecting rib
334: penetrating portion 338: sealing member
400: lower closure portion 410: lower cap
412: lower cap member 414: upper extension
416: locking projection 418: lifting screw rod
420: piston member 422: packing coupling portion
424: piston packing 426: screw rod coupling
428: piston cap
500: first syringe 600: blood
700: second syringe

Claims (15)

An upper container including an extraction tube having a smaller diameter than other parts;
An upper closure portion coupled to close the upper opening portion of the upper container;
A lower container coupled to a lower end of the upper container; And
It includes a lower closure portion coupled to close the lower opening of the lower container,
The upper closing portion is provided with an internal partition plate for partitioning the inside of the upper container at the upper end of the upper closing portion,
The lower closing part is provided with a liftable piston member for adjusting the internal volume of the lower container,
By rotating the lower container with respect to the upper container, an extraction tube closing member provided at an upper end of the lower container closes the lower part of the extraction tube,
The lower container includes a lower container body and a lower container upper body provided on an upper portion of the lower container body, and the extraction pipe closing member is coupled to the upper opening side of the upper body of the lower container through a connecting rib,
The upper container is formed on the upper portion of the extraction tube and includes an upper body having a larger diameter than the extraction tube,
The upper body is connected to a first body, a second body having a diameter smaller than that of the first body, and connected to the first body through a first shaft diameter part, and the second body and a second shaft diameter part. A third body having a smaller diameter than the second body,
The inner partition plate is lowered by partitioning the upper body up and down by descending in a state in contact with the inner circumferential surface of the second body and contacting the upper inner circumferential surface of the second shaft or the third body,
The upper closure part, the upper cap is rotatably coupled to the upper cap engaging projection formed on the outer circumferential surface of the first body and the upper cap for closing the upper end of the upper container, and a support rod provided on the bottom surface of the upper cap and having a thread formed on the outer circumferential surface Including, the inner partition plate is screwed to the support bar is elevated according to the rotation of the upper cap,
The inner partition plate includes an outer circumferential portion contacting the inner circumferential surface of the second body, a support rod coupling portion screwed to the support rod, and an edge penetration portion formed to penetrate up and down at a position greater than a predetermined radius from the center of rotation of the inner partition plate. Including,
When the inner partition plate descends according to the rotation of the upper cap, and the bottom surface of the outer circumference of the inner partition plate contacts the second shaft diameter portion formed on the upper end of the third body, the border penetration portion may include the second It is closed by the shaft diameter portion to divide the upper body up and down and close it,
The support rod is formed with an internal through channel formed through the up and down,
A stopper insertion tube is formed in the extraction tube, and a stopper capable of inserting the needle of the first syringe is coupled to the stopper insertion tube, and blood is injected into the container,
When the needle of the second syringe is inserted into the stopper after the blood centrifugation is completed, a device for centrifuging blood components, characterized in that external air is introduced into the upper container through the internal through channel. According to claim 1,
The upper container is formed in the lower portion of the extraction tube and includes a lower body having a diameter larger than the extraction tube, the lower container is a blood component centrifugal separation device, characterized in that screwed to the lower body. According to claim 1,
The upper body is a blood component centrifugal separation device, characterized in that it comprises a third shaft diameter connecting the third body and the extraction tube. delete delete According to claim 1,
A blood component centrifugation device, characterized in that a check valve is provided on the upper surface of the upper cap to allow air flow from inside to outside of the upper container. delete According to claim 1,
A centrifugal device for blood components characterized in that the internal through-channel is provided with a blocking member including a filter for filtering air. delete According to claim 2,
The lower body includes a lower container coupling part connected to the lower end of the extraction tube, and a lower container coupling part connected to a lower end of the lower container upper insertion part and a thread formed on an inner circumferential surface such that the upper end of the lower container is screwed. A device for centrifuging blood components. delete According to claim 1,
A centrifuge device for blood components, characterized in that a through portion is formed between the connecting ribs. According to claim 1,
A blood component centrifugal separation device, characterized in that a sealing member for sealing a contact surface with the upper container is provided on an outer circumferential surface of the upper body of the lower container. delete The method according to any one of claims 1 to 3, 6, 8 and 10,
The lower closure portion includes a lower cap rotatably coupled to a lower cap coupling protrusion formed on an outer circumferential surface of the lower container, and an elevating screw rod provided at the center of the upper surface of the lower cap, and the piston member includes the lower Centrifuge device for blood components, characterized in that the lifting screw rod rotates and elevates according to the rotation of the cap.

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