KR20190010176A - Centrifugal separation case assembly - Google Patents

Abstract

A centrifugal separator assembly which can mix PRP and buffy coat according to the present invention has an advanced structure so as to minimize the amount of buffy coat which lingers in a syringe and is lost when highly viscous high concentration PRP and buffy coat which are centrifugally separated are extracted by a syringe and injected into a human body. In addition, prevented is displacement of a container constituting the centrifugal separator while blood is centrifugally separated. Moreover, when PPP with low viscosity among the centrifugally separated blood is separated and collected, PPP is prevented from flowing into the container where buffy coat is to be stored.

Description

{CENTRIFUGAL SEPARATION CASE ASSEMBLY} [0001] This invention relates to a centrifuge container assembly capable of mixing a vial and a buffy coat,

The present invention relates to a centrifuge container assembly capable of mixing a platelet rich plasma and a buffy coat, and more particularly, to a centrifugal separation container assembly capable of mixing a centrifugal separator with a high concentration of highly viscous platelet rich plasma (PRP) PRP ") and buffy coat are extracted with a syringe to improve the structure so as to minimize the amount of the buffy coat remaining in the syringe when it is put into the human body and the blood is centrifugally separated from the container (PPP) (hereinafter referred to as " PPP "), which is low in viscosity in the separated blood, is collected and collected, the PPP is prevented from flowing into the container in which the buffy coat is stored To a centrifuge container assembly capable of mixing an improved platelet rich plasma and a Buffy coat.

The blood is composed of red blood cells (RBC), white blood cells (WBC) and platelets (PLT) Platelets are mainly in plasma and plasma is divided into platelet rich plasma (PRP) layer and platelet poor plasma (PPP) layer.

PRP (Platelet Enriched Plasma) is a blood separated from PRP and Buffy coat layers rich in platelet and growth factor by removing RBC by centrifuging its blood, and platelets and white blood cells are highly concentrated have.

These PRPs are relatively located in the lower part of the plasma and contain growth factors such as cytokine, PDGF, TGF-BETA1, and VEGP, and this shows that the PRP has a good effect in skin diseases and wound healing, ought.

Plasma almost free of platelets called PPP (platelet-poor plasma) is used as an autologous blood filler or as a self-plasma derived cosmetic.

When blood is centrifuged at 3000 to 4000 RPM for 3 to 5 minutes, PPP (A), PRP (P), buffy coat (B) (PLT and white blood cell (WBC)), RBC (C). The high concentration of PRP (P) is only a small fraction of collected blood (about 1% of the collected blood) and the buffy coat (B), which is highly viscous and bounded by RBCs, is difficult to collect .

Typically, the blood is taken from the centrifuged container using a syringe to obtain a blood plasma (P) and a high concentration of buffy coat (B), which is injected into the patient.

However, even after the blood P is transferred from the centrifuge container to the syringe, the PRP and the buffy coat (B) are separated from each other as shown in FIG. 2A, Because of its high viscosity, it remains attached to the inner surface of the syringe needle or the like and has a large loss.

FIG. 2B shows the state of PLT and WBC stuck to the inside of the syringe after PRP was cut inside the syringe and then taken with a cotton swab, stained on a slide, and microscopically observed after staining with Wright Stain.

Therefore, the syringe is shaken in advance to the patient to mix the vial coat (B) having a relatively low viscosity with the highly concentrated buffy coat (B) to lower the viscosity of the buffy coat (B) However, since the PRP and the buffy coat are substantially filled in the inside of the syringe, it is difficult to mix the PRP and the buffy coat (B) because there is no clearance space.

For this, as shown in FIG. 3, two syringes are connected to each other, and the blood P and the buffy coat B are flowed in a reciprocating manner and mixed. However, the operation is troublesome and a small amount of buffy The coat (B) is burnt and residual loss occurs.

Further, when the operator takes out the centrifugal blood P and the buffy coat B directly from the centrifugal separator to the syringe, there is a problem that the RBC contacting with the buffy coat B is sucked and taken out together, Is required.

Figs. 4 to 6 show a centrifuge vessel disclosed in Korean Patent No. 1433293, which has a storage portion for easily separating and accommodating the buffy coat.

This is because a receiving space portion 102 for receiving blood is formed at a lower portion and a passage portion 105 having an inner diameter narrower than an upper portion and an upper end opened is formed at an upper portion of the accommodation space portion 102, And a separating space 104 for separating the blood separated by the centrifugal separation and disposed on the lower side of the accommodating space part 102 so as to be pushed upward, And a coupling part 310 detachably coupled to the upper part of the body 100 and coupled to the upper part of the passage part 105 so as to communicate with the interior of the body part 100 when the body 100 is coupled to the lower part of the body 100, And a reservoir 320 having a reservoir 320 through which the concentrated platelets P are introduced and accommodated in the blood separation layer when the lifting cap 200 is lifted, 300).

The separation space 104 is divided into a space 100 and a receiving space 102 via a passage 105. A part of the separation layer of the blood separated by the centrifugation of the blood is lifted And is structured to be introduced and accommodated through the passage portion 105 when the stopper 200 is lifted.

When the centrifugal separation is completed, the upper cap 300 is rotated and raised to move the coupling part 310 and the passage part 105 upward so as to be spaced apart from each other, And separates the uppermost PPP (W) into the separation space 104.

6, the upper cap 300 is lowered again to engage the engaging portion 310 with the end portion of the passage portion 105. When the lifting cap 200 is lifted up, P to the storage unit 320, and takes out the buffy coat P from the syringe.

However, the above-described configuration has the following problems.

7, when the container is rotated at a high speed in the centrifugal separator, the coupling part 310 coupled to the inside of the upper cap 300 is disengaged and falls to the lower side of the passage part 105 have.

In this case, there is a problem that the upper cap 300 is opened and the coupling part 310 is re-coupled again, and the blood is contaminated.

Secondly, a very small amount of blood from the centrifuged blood forms a boundary with the red blood cells R. When the buffy coat P is separated into the storage part 320 by the lifting of the lifting plug 200, In order to minimize the loss of the buffy coat P located at the boundary portion between the red blood cell R and the red blood cell R, the passage portion 105 is formed to be thin and long so that the cross sectional area of the boundary portion with the red blood cell R is reduced to minimize the loss of the buffy coat P. .

That is, when the buffy coat (P) is introduced into the human body, the red blood cells (R) are mixed, causing the pain, so the red blood cells (R) and the buffy coat (P)

5, the lifting cap 200 is lifted up to separate the PPP (W) separated from the separated blood at the top of the separation space 104, as shown in FIG. 5, The PPP W is discharged from the end portion of the passage portion 105 and the storage portion (not shown in the drawing) 320).

That is, since the upper end of the elongated passage portion 105 and the upper end of the storage portion 320 are adjacent to each other, when the PPP (W) is separated into the separating and storing portion 104, the buffy coat P There is a problem that the PPP (W) flows into the storage unit 320 to be stored.

Korea Patent No. 1433293

The present invention has been made to solve the above problems, and it is an object of the present invention to provide a PRP and a buffy coat having a container capable of mixing PRP and buffy coat by separating PRP and buffy coat (PLT and WBC) from PPP and red blood cells, The present invention provides a centrifuge container assembly capable of mixing a Buffy coat.

It is also an object of the present invention to provide a centrifuge container assembly capable of mixing a PRP and a Buffy coat so that a container for storing a buffy coat can be prevented from being separated from an upper cap during centrifugal separation.

Another object of the present invention is to provide a centrifuge container assembly capable of mixing a PRP and a Buffy coat so as to prevent PPP from flowing into a container in which a buffy coat is to be stored when PPP is separated into a separating and storing unit I have to.

It is another object of the present invention to provide a centrifuge container assembly capable of mixing a PRP and a Buffy coat so as to extract the PRP and the buffy coat from the container as much as possible without remaining.

Yet another object of the present invention is to provide a method of mixing a PRP and a Buffy coat to minimize the amount of a buffy coat contacting a RBC in a centrifuge container to reduce the amount of a buffy coat that can not be separated and stored in a storage portion of a container And to provide a possible centrifuge container assembly.

The present invention for achieving the above object is a centrifuge container assembly for centrifuging blood with red blood cells (RBC), PRP and Buffy coat, PPP,

Wherein a separation space portion is formed in the upper portion to receive a part of the separation layer of blood separated by centrifugal separation of the blood and is formed at a boundary between the accommodation space portion and the separation space portion, And a passage tube through which the separation layer of the separated blood passes is formed;

A lift cap which is movably coupled to the lower portion of the accommodation space and lifts the blood separated by the centrifugal separation and discharges the blood to the passage pipe;

An upper cap detachably coupled to an upper portion of the container body;

And a container disposed inside the upper cap, the PRP and the buffy coat in the separation layer of blood from the passage tube being introduced and received, and the upper end being coupled to the inner surface of the upper cap,

Wherein the container has a concave shaped storage portion into which the PRP and the buffy coat are introduced and accommodated, and an inlet pipe connected to the passage pipe to guide the PRP and the buffy coat to the storage portion,

Wherein the inlet pipe is formed to be vertically long from the lower end to the upper end of the storage part, the lower end is seated on the upper end of the passage pipe, the upper end is connected to the storage part,

And the volume of the storage portion is formed to be larger than the volume of the PRP and the buffy coat.

Further, in the assembly of the present invention, the upper end of the container is integrally formed on the inner surface of the upper cap.

Further, in the assembly of the present invention, the inner peripheral surface of the lower end of the inflow pipe is provided with a coupling step to be coupled to and supported by the upper end of the passage pipe, thereby preventing the container from falling to the separation space.

Further, in the assembly of the present invention, a peripheral edge is formed around the inner circumference of the upper end of the inflow pipe, so that the cross-sectional area of the interface between the buffy coat and the RBC is reduced.

In addition, in the assembly of the present invention, the upper cap has a supply port for supplying a blood to the accommodation space part by a syringe being introduced into an upper surface thereof, and a syringe for extracting the PRP and the buffy coat, And a possible outlet is formed.

Further, in the assembly of the present invention, the bottom of the storage portion is formed as an inclined surface inclined in one direction,

And a collecting groove in which the PRP and the buffy coat are collected and the end of the needle of the syringe enters is further formed on the lowest side of the inclined surface.

First, the volume of the storage section 41 of the container 40 is made larger than the volume of the separated PRP and buffy coat, thereby enabling the mixing of the PRP and the buffy coat in the storage section 41. This makes it possible to mix the high viscosity PRP and the relatively low viscosity buffy coat 72 in the container 40 so that the viscosity is lowered so that the PRP and the buffy coat 72 are extracted by the later syringe and injected into the human body, The amount of loss of the PRP and the buffy coat 72 remaining in the PRP and the buffy coat 72 can be reduced.

Secondly, since the inflow pipe 42 of the container 40 is formed to have a length corresponding to the depth of the storage part 41, the PPP 71 is discharged from the end of the path tube 13 during the rapid ascending operation of the lifting tool It is possible to prevent the PPP 71 from entering the storage portion 41 of the container 40 through which the buffy coat 72 is to be stored through the connection hole 42a.

Thirdly, the peripheral edge 42c is formed around the inner peripheral surface of the upper end portion of the inlet pipe 42 of the container 40, so that the sectional area of the interface between the buffy coat 72 and the RBC 71 is reduced. The amount of the buffy coat 72 that can not be separately stored in the storage unit 41 can be reduced by minimizing the amount of the coat 72. [

Fourthly, a collecting groove 41a in which the buffy coat 72 is collected on the lowermost side of the bottom inclined surface of the container 40 and into which the end of the needle of the syringe enters, and a small amount of PRP and buffy coat 72, So that the needle is collected in the collecting groove 41a and the needle of the syringe 1 enters the collecting groove 41a to be extracted to the maximum extent without remaining in the storing part 41. [

Fifthly, when the container 40 is detachably coupled to the upper cap 30, the upper end of the passage tube 13 is inserted into the lower end engaging tab 42b of the inflow pipe 42 and is engaged therewith, The container 40 is prevented from being separated from the upper cap 30 and falling to the separation space portion 12 side during operation.

1 is a schematic view showing a state in which PRP and a buffy coat are extracted from a conventional centrifuge container by a syringe,
2A is a cross-sectional view of a state in which a PRP and a buffy coat are extracted in a syringe,
FIG. 2B shows an enlarged photograph of a PRP stained with Wright Stain after microscopic observation after cutting the inside of a syringe after the injection and collecting it with a swab,
3 is a schematic view showing a state in which PRP and buffy coat extracted in a conventional syringe are mixed,
FIGS. 4 to 6 are operation states showing operation states of extracting the PRP and the buffy coat from the conventional centrifugal separator,
FIG. 7 is a schematic view showing the problem during centrifugal separation of the centrifugal separator of FIG. 4,
Figure 8 is a perspective view of a centrifuge container assembly of an embodiment of the present invention,
Fig. 9 is a sectional view of Fig. 8,
10 is an exploded perspective view of FIG. 8,
11A to 11F are operation state diagrams showing a process of extracting a PRP and a buffy coat using a centrifuge vessel of the embodiment of the present invention,
12 is a cross-sectional view of another embodiment of the present invention.

The centrifuge container assembly capable of mixing the PRP and Buffy coat of the present invention extracts centrifuged high concentration PRP and buffy coat with a syringe to remove PRP and buffy coat residue And prevents the containers constituting the container assembly from falling out during the centrifugal separation of the blood. When separating and collecting the PPP among the separated blood, the container in which the buffy coat is to be stored is loaded with PPP So that it can be prevented from being introduced.

Referring to FIGS. 8 to 10, the centrifuge container assembly of the present invention, in which blood is centrifuged with red blood cells (RBC), PRP and Buffy coat, and PPP, comprises a container body 10, An upper cap 30 screwed to an upper end of the body 10; a container 40 disposed inside the upper cap 30 to store the PRP and the buffy coat; And a lifting cap 20 which is coupled to the lifting cap 20 in a liftable manner.

The container body 10 is formed with a receiving space part 11 for receiving blood and a separation space part 12 for accommodating the PPP separated by centrifugal separation of the blood, And a passage tube (13) formed at a boundary between the space portion and the separation space portion and formed to narrow the passage from the lower portion to the upper portion and having an upper opening to allow the separated blood to pass therethrough.

The lifting cap 20 is lifted and lowered to the lower portion of the accommodation space portion 11 so that blood separated by centrifugal separation is lifted and discharged to the passage tube 13. [

A lower cap 25 having a central hole 25a is screwed into a lower portion of the container body 10 so as to allow an elevating mechanism to push up and lift the elevating stopper 20 upward, Thereby preventing escape.

The upper cap 30 is detachably fastened to the upper portion of the container body 10 and includes a supply port 31 and an outlet port 32 formed of a thin rubber material so that the injection needle can be inserted and re- do.

Blood is supplied to the accommodation space portion 11 through the supply port 31 and the PRP and the buffy coat separated into the storage portion 41 are extracted to the outside through the outflow port 32. [

The container 40 is integrally formed or detachably coupled to the inside of the upper cap 30, and the PRP and the buffy coat are received and accommodated from the passage pipe 13.

The container 40 includes a recess 41 having a concave shape in which the PRP and the buffy coat are received and accommodated, and a guide 41 connected to the passage pipe 13 to guide the PRP and the buffy coat to the storage unit 41 And an inflow pipe (42).

The inlet pipe 42 is vertically formed up and down from the lower end to the upper end of the storage unit 41. An engagement step 42b is formed on the inner surface of the lower end to be seated on the upper end of the passage pipe 13, A connection hole 42a is formed so as to be connected to the connection portion 41. [

The volume of the storage part 41 is relatively larger than the volume of the PRP and the buffy coat so that the PRP and the buffy coat can be mixed in the storage part 41 when the container body 10 is shaken.

The upper end of the passage tube 13 is engaged when the upper cap 30 is rotated and lowered. This is because when the container 40 is detachably coupled to the upper cap 30, the container 40 is separated from the upper cap 30 during centrifugal separation at high speed (as described in FIG. 7) To the separation space portion (12) side.

12, a circumferential step 42c is formed around the inner circumferential surface of the upper end of the inflow pipe 42. As shown in FIG. In this case, the cross-sectional area of the interface between the buffy coat 72 and the RBC 71 is reduced so that the amount of the buffy coat 72 contacting the RBC 71 is minimized and stored in the storage unit 41 72) in the case of the present invention.

The bottom of the storage unit 41 is formed as a sloped surface inclined in one direction and a collecting groove 41a in which the PRP and the buffy coat 72 are collected and the end of the syringe needle is introduced is formed at the lowest side of the inclined surface . This allows the PRP and the buffy coat 72, which are small in viscosity, to be extracted to the maximum extent without being retained in the storage part 41.

The blood separation operation using the centrifuge container assembly of the embodiment of the present invention having the above-described configuration is as follows.

[Blood Supply and Centrifugation]

11A, after the syringe 1 having the blood 70 of about 30 to 60 cc is passed through the supply port 31, the storage space 11 of the container body 10 is inserted through the passage tube 13, And the blood 70 injected into the accommodation space portion 11 is centrifuged using a centrifugal separator.

At this time, the upper cap 30 is coupled to the container body 10 so that the upper end of the passage pipe 13 enters the lower end joining step 42b of the inflow pipe 42.

11B, the blood 70 is centrifugally separated from the bottom of the accommodation space 11 by the RBC 71, the PRP, and the buffy coat 72 by rotating the blood at a high speed for 3 to 5 minutes at 3,000 to 4,000 RPM. And a PPP (73).

At this time, when the container 40 is detachably coupled to the upper cap 30, the upper end of the channel tube 13 enters and is coupled to the lower end joining jaw 42b of the inlet pipe 42, Thereby preventing the container 40 from being separated from the upper cap 30 and falling to the separation space portion 12 side during the separation operation.

[Separation of PPP]

11C, the upper cap 30 is rotated so that the lower end of the inflow pipe 42 and the upper end of the path tube 13 are separated from each other so that the accommodation space portion 11 and the separation space portion 12 are communicated with each other do.

The lifting cap 20 is lifted up from the inside of the accommodating space part 11 by moving the lifting device (not shown) into the central hole 25a of the lower cap 25 and pushing the lifting device 20 upward, The PPP layer 71 located at the uppermost one of the separation layers of the blood stored in the accommodation space part 11 is pushed into the separation space part 12 through the path tube 13.

At this time, since the inflow pipe 42 is formed to have a length corresponding to the depth of the storage unit 41, even if the PPP 71 is discharged from the end of the passage pipe 13 during the sudden upward movement of the lifting tool, So that it can be prevented from entering the storage part 41 of the container 40 through the connection hole 42a.

[Separation of PRP and buffy coat]

11D, the upper cap 30 is rotated to allow the upper end of the passage pipe 13 to enter the lower end engaging tab 42b of the inflow pipe 42 so that the accommodating space portion 11 and the separation space portion 12 are blocked.

The passage tube 13 and the storage portion 41 of the container 40 are communicated with each other by a connection hole 42a.

11E, when the lift cap 20 is lifted for a second time using the lifting tool, the PRP and the buffy coat 72 are lifted up from the container 40 through the connection hole 42a formed in the upper end of the inflow pipe 42, And is stored in the storage part 41 of 10 to 30 cc.

12, since the cross-sectional area of the interface between the buffy coat 72 and the RBC 71 is reduced by forming the circumferential step 42c around the inner circumference of the upper end of the inflow pipe 42, the RBC 71 The amount of the buffy coat 72 that can not be separately stored in the storage unit 41 can be reduced.

That is, since the buffy coat 72 separated on the upper side of the RBC 71 is small and it causes pain when the RBC 71 is mixed into the human body during the injection, the circumferential jaw 42c is formed, It is possible to minimize the amount of the buffy coat 72 remaining and lost in the buffy coat 72.

[PRP and Buffy Coat Mixing]

11E, the PRP and the buffy coat 72 are flown into the storage portion 41 from the path tube 13, and then the container body 10 is shaken and the PRP and the buffy coat 72 ).

By mixing the high-viscosity buffy coat and the relatively low-viscosity PRP, the viscosity is lowered so that the buffy coat 72 is extracted by the later syringe and is injected into the human body, so that the loss of the remaining buffy coat 72 in the syringe 1 can be reduced do.

[PRP and Buffy coat extraction]

11F, the syringe 1 is passed through the outlet 32 of the upper cap 30 to enter the inside of the storage part 41, and then the mixed PRP and the buffy coat 72 are extracted.

At this time, the bottom of the storage unit 41 is inclined in one direction so that the PRP and the buffy coat 72 are collected on one side of the storage unit 41 to reduce the amount of the remaining PRP and the buffy coat 72 .

In particular, as shown in Fig. 12, a collecting groove 41a in which the PRP and the buffy coat 72 are collected on the lowest side of the inclined surface and the end of the needle of the syringe enters is formed, and PRP and buffy The coat 72 is caused to flow along the inclined surface to be collected in the collecting groove 41a so that the needle of the syringe 1 enters the collecting groove 41a and is extracted to the maximum extent without remaining in the storing part 41. [ do.

The centrifuge container assembly of the present invention as described above minimizes the loss of centrifuged PRP and buffy coat residues in the syringe and the centrifuge container and minimizes loss of the container constituting the container assembly during the centrifugal separation of blood And prevents PPP from flowing into a container in which the buffy coat is stored when separating and collecting PPP (platelet rich plasma) among the separated blood.

10 ... container body 11 ... accommodating space part
12 ... separation space part 13 ... passage tube
20 ... lift cap 30 ... upper cap
40 ... container 41 ... storage unit
42 ... inlet pipe

Claims (6)

A centrifuge container assembly for centrifuging blood with red blood cells (RBC), PRP and Buffy coat, PPP,
And a separation space part 12 in which the PPP separated by the centrifugal separation of the blood is accommodated is formed in an upper part of the separation space part 12. A boundary between the accommodation space part and the separation space part is formed, A container body 10 having a passage tube 13 formed to be narrowed from a lower portion to an upper portion and having an upper opening and through which the separated blood passes;
A lift cap (20) movably coupled to a lower portion of the accommodation space (11) for lifting the blood separated by the centrifugal separation and discharging the blood to the passage tube (13);
An upper cap 30 detachably coupled to the upper portion of the container body 10;
And a container 40 disposed inside the upper cap 30 and containing the PRP and the buffy coat from the passage tube 13 and having an upper end coupled to an inner surface of the upper cap 30 However,
The container 40 includes a recess 41 having a concave shape in which the PRP and the buffy coat are received and accommodated, and a guide 41 connected to the passage pipe 13 to guide the PRP and the buffy coat to the storage unit 41 An inlet pipe 42,
The inflow pipe 42 is vertically formed up and down from the lower end to the upper end of the storage unit 41. The lower end of the inflow pipe 42 is seated on the upper end of the passage pipe 13 and the upper end is connected to the storage unit 41 A connection hole 42a is formed,
Wherein the volume of the reservoir (41) is relatively larger than the volume of the PRP and the buffy coat. The centrifuge container assembly of claim 1, wherein the upper end of the container (40) is integrally formed on the inner surface of the upper cap (30). The container according to any one of claims 1 to 4, wherein an inner peripheral surface of the lower end of the inflow pipe (42) is provided with a coupling step (42b) coupled to and supported by an upper end of the passage pipe (13) Wherein the PRP and the Buffy coat are capable of mixing. The air conditioner according to any one of claims 1 to 3, wherein a circumferential step (42c) is formed around the inner circumferential surface of the upper end of the inflow pipe (42)
Wherein a cross-sectional area of the interface between the buffy coat and the RBC is reduced. The upper cap (30) according to any one of claims 1 to 3, wherein the upper cap (30) has a supply port (31) for introducing blood into the accommodation space (11) (PRP) and a Buffy coat (32) capable of mixing into a PRP and a buffy coat. The storage unit (41) according to any one of claims 1 to 3, wherein a bottom of the storage unit (41) is formed as an inclined surface inclined in one direction,
And a collection groove (41a) in which a PRP and a buffy coat are collected and an end of a syringe needle is introduced are further formed at the lowest side of the inclined surface, wherein the PRP and the Buffy coat are mixable.

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