KR102223877B1 - Centrifugal separation case assembly - Google Patents

Abstract

In the present invention, the centrifugal separator assembly can minimize the amount of buffy coat that is lost by remaining in the syringe when the centrifuged high concentration and high viscosity PRP and buffy coat are extracted with a syringe and injected into the human body. The structure is improved so that it is possible to prevent dropping of components due to centrifugal force during the process of centrifuging blood, and to prevent PPP from flowing into the storage unit 35 where the buffy coat is stored in the layered blood. .

Description

Centrifugal separator assembly {CENTRIFUGAL SEPARATION CASE ASSEMBLY}

The present invention relates to a centrifuge container assembly, and in particular, a high concentration and high viscosity PRP (PRP) (hereinafter referred to as'PRP') and a buffy coat are extracted with a syringe to the human body. The structure has been improved to minimize the amount of buffy coat that remains in the syringe and is lost when injected into the syringe, prevents the container from dropping out during the centrifugation process of blood, and has a low viscosity in the layered blood. When collecting and separating PPP (platelet Poor Plasma) (hereinafter referred to as'PPP'), the improved PRP and buffy coat can be mixed to prevent PPP from entering the container where the buffy coat will be stored. It relates to a container assembly.

Blood is composed of red blood cells (RBC) (hereinafter referred to as'RBC'), white blood cells (WBC) (hereinafter referred to as'WBC'), platelets (hereinafter referred to as'PLT') and plasma.

PRP (Platelet-Enriched Plasma) is blood obtained by centrifuging its own blood to remove RBCs and separating platelets and a layer of Buffy coat rich in growth factors, and has high concentration of platelets and white blood cells.

These PRPs are relatively located in the lower part of plasma and contain growth factors such as cytokines, PDGF, TGF-BETA1, and VEGP. Is losing.

In addition, plasma without platelets, called PPP (platelet-lean plasma), is used as an autologous blood filler or as an autologous plasma-derived cosmetic.

When the blood is centrifuged for 3 to 5 minutes at 3000 to 4000 RPM, as shown in Fig. 1, PPP (A), PRP (P), buffy coat (B) (PLT and white blood cells (WBC)), RBC from the top of the container (C) It is separated in order, and the highly concentrated PRP(P) is only a small amount of the collected blood (about 1% of the collected blood), and the buffy coat (B), which has a high viscosity and borders the RBC, is difficult to collect. .

Normally, PRP (P) and highly concentrated buffy coat (B) are obtained from a container in which blood is centrifuged using a syringe and injected into a patient.

However, even after the PRP (P) is transferred from the centrifuge container to the syringe, as shown in Fig. 2A, PRP and the buffy coat (B) remain in a separate layer, and the buffy coat (B) remains after injection into the patient. Due to its high viscosity, it remains attached to the inner surface of the syringe needle, resulting in a large loss.

Figure 2b shows the shape of the PLT and WBC sticking to the inside of the syringe after injection of PRP, cutting the inside of the syringe, collecting it with a cotton swab, smearing it on a slide, and observing it under a microscope after staining with Wright Stain.

Therefore, the buffy coat (B) remaining in the syringe is lowered by lowering the viscosity of the overall buffy coat (B) by mixing the relatively low viscosity PRP (P) and the highly concentrated buffy coat (B) by shaking the syringe before injection into the patient. Although it induces loss prevention, it is difficult to mix PRP and buffy coat (B) because there is no free space since the inside of the syringe is substantially filled with PRP and buffy coat.

To this end, as shown in Fig. 3, two syringes are butt-connected, and the PRP (P) and the buffy coat (B) are mixed by reciprocating flow, but there is trouble in the operation, and a small amount of buffy is used in the butted syringe. The coat (B) is smeared, resulting in residual loss.

In addition, when the operator directly takes out the centrifuged PRP (P) and the buffy coat (B) from the centrifuge container with a syringe, there is a problem that the RBC in contact with the buffy coat (B) is sucked and taken out. The skill level of is required.

4 to 6 are disclosed in Korean Patent Registration No. 1443293, and show a centrifugal separator having a storage unit so that the buffy coat can be easily separated and accommodated.

In this case, a receiving space portion 102 for receiving blood is formed in the lower portion, and a passage portion 105 with an inner diameter narrower than the lower portion and an open upper end is formed at the upper portion of the receiving space portion 102, and a separation space portion ( The body 100 having the body 100 formed therein, and the body 100, which is arranged to be elevated at the lower part of the receiving space part 102, is lifted to push the separation layer of blood separated by centrifugal separation upward to inflow into the separation space part 104 The stopper 200 and the coupling portion 310 that is detachably coupled to the upper portion of the body 100 and coupled to the upper end of the passage portion 105 to communicate with the inside of the passage portion 105 when coupled with the body 100 at the lower center of the center It is formed and is formed to extend below one side of the coupling part 310 so that when the lifting stopper 200 rises, the upper cap having a storage part 320 in which the concentrated platelets P are introduced and accommodated in the blood separation layer ( 300).

Separation space part 104 is a structure partitioned by a passage part 105 from the receiving space part 102 on the upper side of the body 100, and a part of the separation layer of blood separated by centrifugation of the blood is raised and lowered. When the stopper 200 is raised, it is introduced and received through the passage part 105.

When the centrifugal separation is completed, as shown in FIG. 5, the upper cap 300 is rotated and raised to move the coupling part 310 and the passage part 105 upward so that they are spaced apart from each other, and the lifting stopper 200 is raised. To separate the uppermost layer of PPP (W) into the separation space (104).

Subsequently, as shown in FIG. 6, the upper cap 300 is lowered again to engage the coupling part 310 to be fitted to the end of the passage part 105, and the buffy coat ( P) is moved to the storage unit 320 and stored, and the buffy coat (P) is taken out with a syringe.

However, the above configuration has the following problems.

First, when the container is rotated at high speed in a centrifuge, as shown in FIG. 7, the coupling part 310 coupled to the inner side of the upper cap 300 is separated and falls down to the lower side of the passage part 105. have.

In this case, there is a problem of contaminating blood and hassle to re-couple the coupling portion 310 by opening the upper cap 300.

Second, the buffy coat (P), which is a very small amount of the centrifuged blood, forms a boundary with the red blood cells (R), and when the buffy coat (P) is separated into the storage unit 320 by the rise of the elevator stopper (200), the red blood cells In order to minimize the loss of the buffy coat (P) located at the boundary with the (R), the passage portion 105 is formed thin and long to reduce the cross-sectional area of the boundary with the red blood cells (R) to minimize the loss of the buffy coat (P). I'm doing it.

In other words, when the buffy coat (P) is injected into the human body, if the red blood cells (R) are mixed, it causes pain, so the buffy coat (P) at the interface with the red blood cells (R) is inevitably lost.

For the above reasons, the passage part 105 must be formed to be thin and long, so the separated PPP (W) on the uppermost part of the separated blood is raised by raising the lifting stopper 200 as shown in FIG. 5 to provide the separation space part 104. When separating into, the rising pressure acts on the elongated passage 105 even with a small lifting force of the lifting stopper 200, so that the PPP(W) is discharged from the end of the passage 105, so that the storage unit ( 320).

That is, the registered patent is because the upper end of the elongated passage part 105 and the upper end of the storage part 320 are adjacent to each other, so when the PPP (W) is separated into the separate storage part 104, the buffy coat (P) is There is a problem in that the PPP(W) flows into the storage unit 320 to be stored.

Korean Patent Registration No. 143293

The present invention was created to solve the above problems, and formed by closing the storage portion in which PRP and the buffy coat are stored between the upper cap and the intermediate cap that are mutually coupled, so that the high viscosity PRP and the relatively low viscosity buffy coat A centrifugal separator assembly is provided to reduce the viscosity of PRP and buffy coat by allowing mixing in the storage unit to reduce the amount of loss of PRP and buffy coat remaining in the syringe after extracting PRP and buffy coat with a syringe and injecting it into the human body. It has its purpose.

Another object of the present invention is to provide a centrifugal separator assembly capable of preventing PPP from flowing into the storage unit when PPP is separated into a separation space, and minimizing loss of the buffy coat flowing into the separation space.

Another object of the present invention is to provide a centrifugal separator assembly capable of minimizing the incorporation of red blood cells in contact with the buffy coat into the storage unit when the buffy coat is extracted into the storage unit.

The present invention for achieving the above object is a centrifugal separator assembly for centrifuging blood with red blood cells (RBC), PRP and buffy coat, and PPP,

The lower part is opened and the receiving space part for receiving blood is formed, the PPP separated by centrifugation of the blood is received at the upper part, and a separation space part having a first male screw formed on the outer circumferential surface of the upper part is formed, and the receiving space part and the separation space A tubular container body that divides the portion and is formed to narrow a passage from the lower portion to the upper portion and has a first passage pipe through which the separated blood of the receiving space portion passes;

An elevating stopper coupled to a lower portion of the receiving space to be elevating and discharging the blood separated by the centrifugal separation to the first passage pipe;

Elevating means for raising the elevating stopper;

A lower coupling portion screwed to the upper end of the container body and having a first female screw, an upper coupling portion extending upward from the lower coupling portion and having a second male screw formed on an outer circumferential surface, and a bottom formed at an inner lower end of the lower coupling portion A plate, a second passage pipe formed through the bottom plate and having a lower end portion detachably connected to the first passage pipe, and the PRP and burpee formed inside the upper coupling portion and passing through the second passage pipe An intermediate cap having a storage unit in which the coat is stored and connecting or separating the first passage pipe and the second passage pipe;

And an upper cap having a handle portion having an upper surface closed and screwed with the upper coupling portion and having a second female screw formed on an inner circumferential surface thereof to open and close the upper end of the second passage pipe; and

The lifting means includes a cylindrical handle having a third male screw formed on an outer circumferential surface of the lower end of the container body, and having a third female screw screwed to the third male screw of the container body on the inner surface, and disposed on the inside of the handle and the upper end thereof With a lifting rod having a push rod coupled to the lifting stopper,

The lifting stopper coupled to the push rod is raised and lowered by the rotation of the lifting rod,

A circumferential jaw is formed on the inner surface of each upper end of the first passage pipe and the second passage pipe,

It is characterized in that the cross-sectional area of the interface between the buffy coat and the RBC is reduced.

In addition, in the assembly of the present invention, the first passage pipe and the second passage pipe are formed to have a smaller diameter toward the top,

It has an elastic stopper coupled to the upper surface of the upper cap,

The elastic stopper is characterized in that the supply cap portion for supplying blood to the receiving space portion by entering the injection needle, and an outlet cap portion capable of entering the injection needle for extracting the PRP and buffy coat stored in the storage portion to the outside is formed. do.

In addition, the bottom plate is characterized in that the concave formed.

First, mixing the high-viscosity PRP and the relatively low-viscosity buffy coat (P) in the storage unit 35 by making the storage unit 35 formed between the upper cap 40 and the intermediate cap 30 closeable. By lowering the viscosity, it is possible to reduce the amount of loss of PRP and buffy coat (P) remaining in the syringe after extracting PRP and buffy coat (P) with a syringe and injecting it into the human body.

Second, even if the container assembly of the present invention is rotated at high speed, the storage unit 35 in which the PRP and the buffy coat (P) are stored is formed by the combination of the upper cap 40 and the intermediate cap 30, so that the prior art It solves the problem that the container 40 is separated from the upper cap 30 and falls toward the separation space 12.

Third, when separating the PPP (P) into the separation space 12, the upper end of the second passage pipe 34 is closed by the upper cap 40, so that the PPP Even if (P) is discharged from the end of the first passage pipe (13), it is possible to prevent the PPP (P) from flowing into the storage unit (35).

Fourth, since the lifting rod 60 is raised and lowered by the rotation of the operator, it is possible to prevent a sudden rise of the lifting stopper 20 and fine adjustment is possible, as well as the PPP (W) and the buffy coat ( Since the boundary of P) is reduced, it is possible to minimize the loss of the buffy coat P flowing into the separation space 12 side.

Fifth, when the cross-sectional area of the interface between the buffy coat (P) and the red blood cells (R) is reduced by the circumferential jaw (34a), when the buffy coat (P) is extracted to the storage unit 35 when the elevator stopper (20) is raised, the buffy coat (P) is extracted. It is possible to minimize the incorporation of red blood cells R in contact with (P) into the storage unit 35.

1 is a schematic diagram showing a state of extracting PRP and buffy coat from a conventional centrifuge container with a syringe;
Figure 2a is a cross-sectional view of a state in which PRP and buffy coat are extracted from a syringe;
Figure 2b is an enlarged photograph of PRP after injection, the inside of the syringe was cut, collected with a cotton swab, smeared on a slide, and observed under a microscope after staining with Wright Stain;
3 is a schematic diagram showing a state of mixing PRP and buffy coat extracted in a conventional syringe;
4 to 6 are operational state diagrams showing an operating state of extracting PRP and buffy coat from another conventional centrifuge container,
7 is a schematic diagram showing a problem during centrifugation use of the centrifuge container of FIG. 4;
8 is an exploded perspective view showing a centrifugal separator assembly according to an embodiment of the present invention;
9 is an exploded cross-sectional view of FIG. 8;
10 is a cross-sectional view illustrating a state in which blood collected in a centrifuge container assembly according to an embodiment of the present invention is injected and centrifuged;
11 is a view for explaining a state in which the upper end of the first passage pipe is opened and the PPP is separated;
12 is a view showing a process of opening the upper end of the second passage pipe and extracting PRP and buffy coat.

The centrifugal separator assembly of the embodiment of the present invention allows mixing of PRP and buffy coat, so that the centrifuged high concentration of PRP and buffy coat are taken out with a syringe, and the PRP remains in the syringe and is lost when injected into the human body. The structure is improved to minimize the amount of buffy coat.

In addition, as in the prior art, it solves the problem that the container constituting the container assembly is dropped out during the process of centrifuging blood, and PPP is prevented from flowing into the storage unit 35 in which the layered blood buffy coat is stored. It has an improved structure to prevent it.

8 to 10, the centrifugal separator assembly of the embodiment of the present invention for centrifuging blood with red blood cells (RBC) (R), PRP and buffy coat (P), and PPP (W), The container body 10, the intermediate cap 30 screwed to the upper end of the container body 10, the upper cap 30 coupled to the upper end of the intermediate cap 30, and the container body 10 It includes an elevating stopper 20 coupled to the lower inner side so as to be elevating, and an elevating means for raising the elevating medium 20.

The container body 10 has a cylindrical shape, the lower part is opened and the receiving space part 11 for receiving blood is formed, the PPP(W) separated by centrifugal separation of the blood is accommodated in the upper part, and the upper part is removed on the outer peripheral surface. 1 A separation space part 12 having an external screw 14 formed thereon is formed, and the receiving space part 11 and the separation space part 12 are divided, and the passage is formed to narrow the passage from the bottom to the top, and the receiving space part 11 A first passage pipe 13 through which the separated blood of) passes is formed.

The lifting stopper 20 is coupled to the lower inner side of the receiving space 11 so as to be liftable by a lifting means, and lifts the blood separated by the centrifugal separation and discharges it to the first passage pipe 13. .

The intermediate cap 30 is screwed to the upper end of the container body 10 and has a lower coupling part 31 having a first female screw 31a formed thereon, and extending upward from the lower coupling part 31 and is provided on the outer circumferential surface. 2 The upper coupling portion 32 having the male screw 32a formed thereon, the bottom plate 33 formed at the inner lower end of the lower coupling portion 31, and the bottom plate 33, and the lower end portion A second passage pipe 34 that is detachably connected to the first passage pipe 13, and the PRP and buffy coat formed inside the upper coupling portion 32 and passed through the second passage pipe 34 are stored. It is provided with a storage unit (35).

The intermediate cap 30 connects or separates the first passage pipe 13 and the second passage pipe 34 according to a screwed state with the container body 10.

The volume of the storage unit 35 is formed relatively larger than the volume of the centrifuged PRP and the buffy coat, so that the PRP and the buffy coat can be mixed in the storage unit 35 when the container body 10 is shaken. .

The upper cap 40 is provided with a handle part 41 having an upper surface closed and screwed with the upper coupling part 32 of the intermediate cap 30 and having a second female screw 41a formed on the inner circumferential surface of the second passage. The upper end of the tube 34 is opened and closed.

In addition, an elastic stopper 50 is coupled to the upper cap 40, and the elastic stopper 50 is formed of a thin plate of rubber to enable resealing, and the injection needle enters the receiving space 11 It includes a supply cap part 51 for supplying the product, and an outlet cap part 52 through which an injection needle can enter so that the PRP and buffy coat stored in the storage part 35 can be extracted to the outside.

The lifting means is provided with a third male screw (11a) is formed on the outer circumferential surface of the lower end of the container body 10, and includes a lifting rod (60) screwed to the third male screw (11a).

The lifting rod 60 has a cylindrical handle 61 having a third female screw 61a screwed to the third male screw 11a of the container body 10 on the inner surface thereof, and an inner side of the handle 61 It is disposed and the upper end is provided with a push rod 62 that is coupled with the elevating stopper (20).

The lifting stopper 20 coupled with the pushing rod 62 is raised and lowered by the rotation of the lifting rod 60.

On the other hand, the reference numeral "70" in FIG. 8 is a lower cap, which is screwed to the lower end of the container body 10 to prevent separation of the lifting cap 20 when storing or transporting the container assembly, and the lifting rod 60 When using ), the lower cap 70 is removed and used.

Meanwhile, circumferential jaws 13a and 34a are formed around inner circumferential surfaces of upper ends of the first passage pipe 13 and the second passage pipe 34. In this case, the cross-sectional area of the interface between the PPP (W) and the buffy coat (P) is reduced by the circumferential jaw (13a), so that the PPP (W) is extracted into the separation space part 12 when the lifting stopper 20 is raised. When it becomes possible to minimize the amount of the buffy coat (P) in contact with the PPP (W) is discarded into the separation space (12).

In addition, the cross-sectional area of the interface between the buffy coat (P) and the red blood cells (R) is reduced by the circumferential jaw (34a), so that when the lifting stopper (20) rises, the buffy coat (P) is transferred to the storage unit (35). When extracted, it is possible to minimize the red blood cells (R) in contact with the buffy coat (P) from being mixed into the storage unit (35).

In addition, the bottom plate 33 of the storage unit 35 is formed to be concave, so that a small amount of PRP and buffy coat (P) as a high viscosity do not remain in the storage unit 35 by the injection needle 100 as much as possible. So that it can be extracted.

The blood separation operation using the centrifugal separator assembly of the embodiment of the present invention having the above configuration is as follows.

[Blood supply and centrifugation]

Referring to FIG. 10, after passing the injection needle 100 of a syringe in which blood is stored through the supply cap portion 51, the receiving space portion of the container body 10 ( 11) Blood is supplied to the inside, and the blood injected into the receiving space 11 is centrifuged using a centrifugal separator.

At this time, the first and second passage pipes 13 and 34 are connected, and the upper end of the second passage pipe 34 is closed by the upper cap 40.

When the container assembly is rotated at a high speed for 3 to 5 minutes at 3,000 to 4,000 RPM, blood is centrifuged to form red blood cells (RBC; R), PRP and buffy coat (P) and PPP (W) from the bottom of the receiving space 11. The layers are separated.

Even if the container assembly of the present invention is rotated at a high speed, as shown in FIG. 7, the container 40 in the prior art is separated from the upper cap 30 and falls toward the separation space 12.

[PPP separation water]

Referring to FIG. 11, after layer separation into red blood cells (RBC; R), PRP and buffy coat (P) and PPP (W), the intermediate cap 30 is rotated to prevent the upper end of the first passage tube 13 and The lower end of the second passage pipe 34 is spaced apart so that the accommodation space 11 and the separation space 12 communicate with each other.

Subsequently, when the lifting rod 60 is rotated and the lifting stopper 20 is pushed upward, the lifting stopper 20 moves upward in the accommodation space 11 and a separation layer of blood stored in the accommodation space 11 Among them, the PPP(W) layer located at the top is pushed into the separation space 12 through the first passage pipe 13.

At this time, since the upper end of the second passage pipe 34 is closed by the upper cap 40, even if the PPP (P) is discharged from the end of the first passage pipe 13 when the lifting stopper 20 is rapidly raised, It is possible to prevent the PPP(P) from flowing into the storage unit 35.

In addition, since the lifting rod 60 is raised and lowered by the rotation of the operator, it is possible to prevent a sudden rise of the lifting stopper 20 and fine adjustment is possible, as well as PPP(W) and buffy coat by the circumferential jaw 13a. Since the boundary of (P) is reduced, it is possible to minimize the loss of the buffy coat (P) flowing into the separation space portion 12 side.

[Separate water for PRP and buffy coat]

Referring to FIG. 12, after the PPP (W) of the uppermost layer of the layer-separated blood is separated into the separation space portion 12, the intermediate cap 30 is rotated to form the first passage pipe 13 and the second passage pipe. 34 is connected so that the receiving space 11 and the separation space 12 are blocked.

Subsequently, the upper cap 40 is rotated so that the upper end of the second passage pipe 34 and the bottom surface of the upper cap 40 are spaced apart to communicate with the storage unit 35.

Subsequently, when the lifting rod 60 is rotated to raise the lifting stopper 20 for the second time, the PRP and the buffy coat (P) are introduced into the storage unit 35 through the upper end of the second passage pipe 34 and 10 ~30cc is stored.

In addition, the cross-sectional area of the interface between the buffy coat (P) and the red blood cells (R) is reduced by the circumferential jaw (34a), so that when the lifting stopper (20) rises, the buffy coat (P) is transferred to the storage unit (35). When extracted, it is possible to minimize the red blood cells (R) in contact with the buffy coat (P) from being mixed into the storage unit (35).

That is, the separated buffy coat (P) on the upper side of the RBC (R) is a small amount, and when the RBC (R) is mixed into the human body, it causes pain. Therefore, by forming the circumferential jaw (34a), the upper surface of the RBC (R) It is possible to minimize the amount of the buffy coat (P) remaining in and lost.

[PRP and buffy coat mixing]

As shown in FIG. 12, after the PRP and the buffy coat (P) are introduced into the storage unit 35, the container body 10 is shaken to mix the PRP and the buffy coat (P) in the storage unit 35.

By mixing the high-viscosity buffy coat and the relatively low-viscosity PRP, the viscosity is lowered to reduce the loss of the buffy coat (P) remaining in the syringe after extracting the buffy coat (P) with a syringe and injecting it into the human body.

[PRP and buffy coat extraction]

Referring to FIG. 12, the mixed PRP and buffy coat (P) are extracted after entering the inside of the storage unit 35 by passing the injection needle 100 of the syringe through the outflow cap 52 of the upper cap 40 do.

At this time, since the bottom of the storage unit 35 is formed to be concave, the amount of PRP and the buffy coat P remaining in the storage unit 35 can be reduced.

The centrifugal separator assembly of the present invention as described above minimizes the loss of the centrifuged high concentration PRP and buffy coat remaining in the syringe and centrifuge container, and the container constituting the container assembly is dropped during the process of centrifuging blood. When collecting and separating PPP (platelet rich plasma) from the layered blood, it is possible to prevent PPP from flowing into the storage unit 35 in which the buffy coat is to be stored.

10...container body 11...receiving space
12...Separated space part 13...First passage pipe
20...lift stopper 30...middle cap
34...the second corridor 35...storage
40... upper cap 60... lifting bar

Claims (5)

In the centrifuge container assembly for centrifuging blood with red blood cells (RBC), PRP and buffy coat, and PPP,
Separation space part (12) in which the lower part is opened and the receiving space part (11) for receiving blood is formed, the PPP separated by centrifugation of the blood is accommodated in the upper part, and the first male screw 14 is formed on the outer circumferential surface of the upper part Is formed, partitions the receiving space 11 and the separation space 12, is formed to narrow a passage from the bottom to the top, and passes the separated blood of the receiving space 11 ( 13) and the cylindrical container body 10 is formed;
A lift stopper (20) coupled to a lower portion of the receiving space (11) so as to be able to move up and down, to lift the blood separated by the centrifugal separation and discharge it to the first passage pipe (13);
Elevating means for raising the elevating stopper (20);
A lower coupling part 31 which is screwed to the upper end of the container body 10 and having a first female screw 31a, and a second male screw 32a extending upward from the lower coupling part 31 and formed on the outer circumferential surface thereof. The upper coupling portion 32, the bottom plate 33 formed at the inner lower end of the lower coupling portion 31, and formed through the bottom plate 33, and the lower end portion of the first passage pipe 13 and A second passage pipe 34 that is detachably connected, and a storage unit 35 formed inside the upper coupling portion 32 and in which the PRP and the buffy coat passed through the second passage pipe 34 are stored. And an intermediate cap (30) for connecting or separating the first passage pipe (13) and the second passage pipe (34);
The upper cap (40) is provided with a handle part (41) having a closed upper surface and screwed with the upper coupling part (32) and having a second female screw (41a) formed on an inner circumferential surface to open and close the upper end of the second passage pipe (34). ); and,
The rising means is formed with a third male screw (11a) on the outer peripheral surface of the lower end of the container body (10),
A cylindrical handle 61 having a third female screw 61a screwed with the third male screw 11a of the container body 10 is formed on the inner surface, and the upper end is disposed on the inside of the handle 61 and has an upper end of the lifting stopper. With a lifting rod 60 provided with a push rod 62 coupled to the 20,
The lifting stopper 20 coupled with the pushing rod 62 is raised and lowered by the rotation of the lifting rod 60,
A circumferential jaw (13a) (34a) is formed on the inner surface of each upper end of the first passage pipe (13) and the second passage pipe (34),
Centrifugal separator assembly, characterized in that the cross-sectional area of the interface between the buffy coat and the RBC is reduced. delete delete The method according to claim 1, wherein the first passage pipe 13 and the second passage pipe 34 are formed to have a smaller diameter toward the top,
It has an elastic stopper 50 coupled to the upper surface of the upper cap 40,
The elastic stopper 50 includes a supply cap 51 for supplying blood to the receiving space 11 by entering an injection needle, and an injection for extracting PRP and buffy coat stored in the storage unit 35 to the outside. Centrifugal separator assembly, characterized in that the outflow cap portion 52 is formed in which the needle can enter. The centrifugal separator assembly according to claim 1 or 4, wherein the bottom plate (33) is formed to be concave.

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