CN219615282U

CN219615282U - Ultrafiltration tube structure for exosome extraction

Info

Publication number: CN219615282U
Application number: CN202222553276.5U
Authority: CN
Inventors: 王军红; 王英; 梁颖; 蒋青霞
Original assignee: Hangzhou Yuding Biological Technology Co ltd
Current assignee: Hangzhou Yuding Biological Technology Co ltd
Priority date: 2022-09-26
Filing date: 2022-09-26
Publication date: 2023-09-01
Anticipated expiration: 2032-09-26

Abstract

The utility model relates to the technical field of extraction of exosomes, in particular to an ultrafiltration tube structure for exosome extraction, which comprises an ultrafiltration tube body, wherein the ultrafiltration tube body can be applied to a centrifugal machine, the ultrafiltration tube body comprises a centrifugal tube, a filtering structure for filtering liquid is arranged in the centrifugal tube, and a filtering structure for filtering exosomes is arranged in the centrifugal tube; the filtering structure comprises a filtering membrane and an ultrafiltration membrane component, wherein the filtering membrane is used for intercepting large particle molecules in a urine sample, and the ultrafiltration membrane component is used for intercepting small particle molecules in the urine sample. This an ultrafiltration tube structure for exosome draws compares with prior art, and this structural design is comparatively simple, makes things convenient for producer to shorten its delivery cycle greatly, thereby reduced exosome and drawed the cost to a certain extent, and the setting of this structure, its filtration rate is faster, and is effectual.

Description

Ultrafiltration tube structure for exosome extraction

Technical Field

The utility model relates to the technical field of exosome extraction, in particular to an ultrafiltration tube structure for exosome extraction.

Background

At present, the method for extracting exosomes in the market generally uses imported 3KD,10KD,30KD,50KD and 100KD ultrafiltration tubes, the miRNAS in the exosomes are centrifugally filtered, the imported ultrafiltration tubes are expensive, and the delivery period is long, so that the exosome extraction cost is increased, and therefore, a new ultrafiltration tube for exosome extraction needs to be developed.

Disclosure of Invention

(one) solving the technical problems

Aiming at the defects of the prior art, the utility model provides an ultrafiltration tube structure for extracting exosomes, and solves the problems in the background art.

(II) technical scheme

In order to achieve the above purpose, the utility model is realized by the following technical scheme: a ultrafiltration tube structure for exosome draws, including ultrafiltration tube body and this ultrafiltration tube body can be applied to in the centrifuge, ultrafiltration tube body includes the centrifuging tube, be equipped with in the centrifuging tube and be used for carrying out filterable filtration structure to liquid.

Preferably, a filtering structure for filtering the exosomes is arranged in the centrifuge tube; the filtering structure comprises a filtering membrane and an ultrafiltration membrane component, wherein the filtering membrane is used for intercepting large particle molecules in a urine sample, and the ultrafiltration membrane component is used for intercepting small particle molecules in the urine sample.

Preferably, the filter membrane is positioned at the top end of the centrifuge tube and 1/3 of the centrifuge tube, and the ultrafiltration membrane component is positioned below the filter membrane; the ultrafiltration membrane component comprises an ultrafiltration membrane, and the ultrafiltration membrane is arranged on the wall of the centrifuge tube through a connecting piece. The ultrafiltration tube is provided with a first ultrafiltration membrane, so that the molecular retention of a second ultrafiltration membrane can be better ensured. The second way designs 4 ultrafiltration membranes, so that the speed is faster and the effect is high.

Preferably, the number of the ultrafiltration membranes is four, and the four ultrafiltration membranes are respectively arranged below the connecting piece and form an open ultrafiltration membrane component.

Preferably, the cross section of the ultrafiltration membrane component formed by the four ultrafiltration membranes and the connecting piece is conical, the open end of the ultrafiltration membrane component with the conical cross section is close to the filtration membrane, and the closed end of the ultrafiltration membrane component is opposite to the open end.

Preferably, the pore size of the filtering membrane is O.22um, 0.45um or 0.8um, and the pore size of the ultrafiltration membrane is 3KD,10KD,30KD,50KD or 100KD.

Preferably, the feeding end of the centrifugal tube is provided with a centrifugal tube cover for sealing, and the discharging end of the centrifugal tube is provided with a discharging hole for discharging filtered liquid.

Preferably, the cross-sectional shape of discharge gate is the toper form, the biggest end of discharge gate links to each other with the centrifuging tube, the minimum end of discharge gate is located the below of the biggest end of discharge gate.

Preferably, the inside and outside double-wall is arranged in the tube of the centrifuge tube and is transparent, the outer tube wall of the centrifuge tube is provided with scale marks, and the specification of the centrifuge tube is 15ml and 50ml.

Preferably, the centrifuge tube, the filtering membrane and the ultrafiltration membrane are integrally injection molded.

Description of extraction process of exosomes:

the urine sample is poured into an ultrafiltration tube by using a measuring cup, the ultrafiltration tube is placed in a centrifugal machine, and after large particles specified in the urine sample are filtered firstly by the centrifugal machine rotating at a high speed, the urine sample enters the molecular weight cut-off set by a second ultrafiltration membrane.

This an ultrafiltration tube structure for exosome draws compares with prior art, and this structural design is comparatively simple, makes things convenient for producer to shorten its delivery cycle greatly, thereby reduced exosome and drawed the cost to a certain extent, and the setting of this structure, its filtration rate is faster, and is effectual.

Drawings

FIG. 1 is a schematic diagram of the structure of the present utility model;

FIG. 2 is a schematic view of a filter structure according to the present utility model;

FIG. 3 is a block diagram showing the process of extracting exosomes according to the present utility model.

In the figure: 1. an ultrafiltration tube body; 2. centrifuging tube; 3. a filtering structure; 4. a discharge port; 5. a filtering membrane; 6. an ultrafiltration membrane module; 7. an ultrafiltration membrane; 8. a connecting piece; 9. and centrifuging the tube cover.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments.

Referring to fig. 1 to 3, the present utility model provides a technical solution: the ultrafiltration tube structure for extracting exosomes comprises an ultrafiltration tube body 1, wherein the ultrafiltration tube body 1 can be applied to a centrifugal machine, the ultrafiltration tube body 1 comprises a centrifugal tube 2, an inner wall and an outer wall are arranged in a tube of the centrifugal tube 2 and are transparent, scale marks are arranged on the outer tube wall of the centrifugal tube 2, 15ml and 50ml of the centrifugal tube 2 are required in specification, the centrifugal tube 2, a filtering membrane 5 and an ultrafiltration membrane 7 are integrally formed by injection molding, a centrifugal tube cover 9 used for sealing is arranged at the feeding end of the centrifugal tube 2, a discharge hole 4 used for discharging filtered liquid is arranged at the discharge end of the centrifugal tube 2, the cross section of the discharge hole 4 is in a cone shape, the maximum end of the discharge hole 4 is connected with the centrifugal tube 2, the minimum end of the discharge hole 4 is positioned below the maximum end of the discharge hole 4, a filtering structure 3 used for filtering liquid is arranged in the centrifugal tube 2, and a filtering structure 3 used for filtering exosomes is arranged in the centrifugal tube 2; the filtering structure 3 comprises a filtering membrane 5 and an ultrafiltration membrane component 6, wherein the pore size of the filtering membrane 5 is O.22um, 0.45um or 0.8um, the pore size of the ultrafiltration membrane 7 is 3KD,10KD,30KD,50KD or 100KD, the filtering membrane 5 is used for intercepting large particle molecules in a urine sample, the ultrafiltration membrane component 6 is used for intercepting small particle molecules in the urine sample, the filtering membrane 5 is positioned at the top end of the centrifuge tube 2 and positioned at 1/3 of the centrifuge tube 2, and the ultrafiltration membrane component 6 is positioned below the filtering membrane 5; the ultrafiltration membrane component 6 comprises ultrafiltration membranes 7, the ultrafiltration membranes 7 are arranged on the pipe wall of the centrifuge pipe 2 through connecting pieces 8, the number of the ultrafiltration membranes 7 is four, the four ultrafiltration membranes 7 are respectively arranged below the connecting pieces 8 and form an open ultrafiltration membrane component 6, the cross section of the ultrafiltration membrane component 6 formed by the four ultrafiltration membranes 7 and the connecting pieces 8 is conical, the open end of the ultrafiltration membrane component 6 with the conical cross section is close to the filtration membrane 5, and the closed end of the ultrafiltration membrane component 6 is opposite to the direction of the open end.

The electrical components in the connection are all electrically connected with an external main controller and 380V commercial power, and the main controller can be a conventional known device for controlling a computer and the like.

To sum up, this an ultrafiltration tube structure for exosome draws, when using, compare with prior art, this structural design is comparatively simple, makes things convenient for producer to shorten its delivery cycle greatly, thereby reduced exosome to a certain extent and drawed the cost, and the setting of this structure, its filtration rate is faster, and is effectual.

In the description of the present specification, the terms "connected," "mounted," "fixed," "disposed," and the like are to be construed broadly, and for example, "connected" may be a fixed connection or an indirect connection via an intermediate assembly without affecting the relationship of components and technical effects, or may be an integral connection or a partial connection, as in the case of this example, and as will be apparent to those skilled in the art, the specific meaning of the terms in the present utility model or the utility model may be understood in the specific case.

The foregoing is only a preferred embodiment of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art, who is within the scope of the present utility model, should make equivalent substitutions or modifications according to the technical scheme of the present utility model and the inventive concept thereof, and should be covered by the scope of the present utility model.

Claims

1. A ultrafiltration tube structure for exosome draws, including ultrafiltration tube body (1) and this ultrafiltration tube body (1) can be applied to in the centrifuge, its characterized in that: the ultrafiltration tube body (1) comprises a centrifuge tube (2), a filtering structure (3) for filtering liquid is arranged in the centrifuge tube (2), and a filtering structure (3) for filtering exosomes is arranged in the centrifuge tube (2); the filtering structure (3) comprises a filtering membrane (5) and an ultrafiltration membrane component (6), the filtering membrane (5) is used for intercepting large particle molecules in a urine sample, the ultrafiltration membrane component (6) is used for intercepting small particle molecules in the urine sample, the filtering membrane (5) is positioned at the top end of the centrifuge tube (2) and positioned at 1/3 of the centrifuge tube (2), and the ultrafiltration membrane component (6) is positioned below the filtering membrane (5); the ultrafiltration membrane component (6) comprises an ultrafiltration membrane (7), and the ultrafiltration membrane (7) is arranged on the pipe wall of the centrifuge pipe (2) through a connecting piece (8).

2. Ultrafiltration tube structure for exosome extraction according to claim 1, wherein: the number of the ultrafiltration membranes (7) is four, and the four ultrafiltration membranes (7) are respectively arranged below the connecting piece (8) and form an open ultrafiltration membrane component (6).

3. Ultrafiltration tube structure for exosome extraction according to claim 2, characterized in that: the cross-sectional shape of the ultrafiltration membrane component (6) formed by the four ultrafiltration membranes (7) and the connecting piece (8) is in a conical shape, the opening end of the ultrafiltration membrane component (6) with the conical cross-sectional shape is close to the filtration membrane (5), and the direction of the closed end and the direction of the opening end of the ultrafiltration membrane component (6) are opposite.

4. An ultrafiltration tube structure for exosome extraction according to claim 3, wherein: the pore size of the filtering membrane (5) is O.22um, 0.45um or 0.8um, and the pore size of the ultrafiltration membrane (7) is 3KD,10KD,30KD,50KD or 100KD.

5. The ultrafiltration tube structure for exosome extraction of claim 4, wherein: the centrifugal tube is characterized in that a centrifugal tube cover (9) used for sealing is arranged at the feeding end of the centrifugal tube (2), and a discharging hole (4) used for discharging filtered liquid is arranged at the discharging end of the centrifugal tube (2).

6. The ultrafiltration tube structure for exosome extraction of claim 5, wherein: the cross-sectional shape of discharge gate (4) is the toper form, the biggest end of discharge gate (4) links to each other with centrifuging tube (2), the minimum end of discharge gate (4) is located the below of the biggest end of discharge gate (4).

7. The ultrafiltration tube structure for exosome extraction of claim 6, wherein: the inside and outside double-walled and transparent are arranged in the tube of the centrifuge tube (2), scale marks are arranged on the outer tube wall of the centrifuge tube (2), and the specification of the centrifuge tube (2) is 15ml and 50ml.

8. The ultrafiltration tube structure for exosome extraction of claim 7, wherein: the centrifuge tube (2), the filtering membrane (5) and the ultrafiltration membrane (7) are integrally injection molded.