CN209923230U

CN209923230U - Antibody concentration device

Info

Publication number: CN209923230U
Application number: CN201920524300.8U
Authority: CN
Inventors: 李永刚; 李双林; 蔡帅
Original assignee: Nanjing Fan Bo Biological Technology Co Ltd
Current assignee: Jiangsu Fanbo Biological Products Co.,Ltd.
Priority date: 2019-04-17
Filing date: 2019-04-17
Publication date: 2020-01-10
Anticipated expiration: 2029-04-17

Abstract

The utility model discloses an antibody enrichment facility, including a jar body and toothed chain, the base is installed to the bottom of the jar body, the inlet has been seted up to the top intermediate position of the jar body, the inside upper end of the jar body is provided with first milipore filter, the catheter is installed to the one end of first milipore filter, the other end of catheter inserts at jar internal portion, be provided with the waste liquid guide plate under the first milipore filter, be located install first row of waste pipe on the lower jar of the serving of waste liquid guide plate body, install first valve and second valve on catheter and the first row of waste pipe respectively, install the drain guide plate under the waste liquid guide plate, the drain mouth has been seted up to the positive intermediate position of drain guide plate, the bottom nozzle of catheter is located the drain mouth directly over. The utility model discloses a set up first milipore filter, centrifugal urceolus and centrifugal inner tube, solved the concentrated cycle length of current technique, complex operation, the poor problem of concentrated effect.

Description

Antibody concentration device

Technical Field

The utility model relates to a bio-pharmaceuticals technical field specifically is an antibody enrichment facility.

Background

The antibody is a special protein molecule, is used as a reagent for in vitro diagnosis, a medicament for treating diseases, a ligand of immunoaffinity chromatography and the like, has wide application in the fields of life science research, biotechnology and medicine, particularly, the antibody is used as a core reagent of various immunoassays, plays an important role in the sensitivity and specificity of the result of the immunoassay, and the preparation of the antibody with high specificity and high titer is the basis of the immunological technology, but the antibody of any preparation technology needs to be concentrated.

However, the conventional antibody concentration device has the following disadvantages:

1. the existing antibody concentration device mainly concentrates the antibody through an ultrafiltration membrane, then takes out a sample of the concentrated antibody for detection, takes out the antibody after qualification, and takes out the antibody after disqualification for circular concentration, but the circular concentration mode can not concentrate the antibody more thoroughly, the concentration of the antibody still can not meet the requirements, and because the detection link is added, the concentration period is longer, and the operation is more complicated;

2. the existing antibody concentration device has low concentration efficiency, poor effect, large equipment volume and inconvenient use.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

Not enough to prior art, the utility model provides an antibody enrichment facility has solved the concentrated cycle length of current technique, complex operation, the poor problem of concentrated effect.

(II) technical scheme

In order to achieve the above object, the utility model provides a following technical scheme: an antibody concentration device comprises a tank body and a toothed chain, wherein a base is installed at the bottom end of the tank body, a liquid inlet is formed in the middle position of the top end of the tank body, a first ultrafiltration membrane is arranged at the upper end of the inside of the tank body, a liquid guide pipe is installed at one end of the first ultrafiltration membrane, the other end of the liquid guide pipe is inserted into the tank body, a waste liquid guide plate is arranged under the first ultrafiltration membrane, a first waste discharge pipe is installed on the tank body at the lower end of the waste liquid guide plate, a first valve and a second valve are installed on the liquid guide pipe and the first waste discharge pipe respectively, a liquid guide plate is installed under the waste liquid guide plate, a liquid guide port is formed in the middle position of the liquid guide plate, a bottom pipe orifice of the liquid guide pipe is located right above the liquid guide port, a centrifugal outer barrel is arranged below the liquid guide plate in the tank body, a, the transmission shaft is installed to the bottom of centrifugal urceolus, the bearing is installed to the centre of transmission shaft, the fixed plate is installed to the bottom of the jar body, the whole embedding of bearing is on the fixed plate, driven gear is installed to the bottom of transmission shaft, one side welding of the jar body has solid fixed ring, solid fixed ring's inside has cup jointed centrifugal motor, the driving gear is installed to centrifugal motor's bottom pivot end, the driving gear passes through the chain gear and is connected with the driven gear transmission.

Preferably, one side of the tank body is provided with a material taking port right facing the centrifugal outer cylinder, the upper end of the material taking port is movably hinged with a waterproof cover, and a transparent glass window is arranged in the middle of the waterproof cover.

Preferably, a plurality of second ultrafiltration membranes are arranged on the side surface of the centrifugal inner cylinder.

Preferably, a protective cover is arranged at the upper end of the bearing.

Preferably, the bottom end of the fixing plate is provided with a second waste discharge pipe.

Preferably, the bottom of the side surface of the centrifugal outer cylinder is provided with a waste discharge port.

(III) advantageous effects

The utility model provides an antibody enrichment facility possesses following beneficial effect:

(1) the utility model discloses a set up first milipore filter, centrifugal urceolus and centrifugal inner tube, during the use, solution filters through first milipore filter earlier, and reagent and micromolecule water in the solution fall to the waste liquid guide plate through the milipore filter on, discharge through first row of waste pipe, and the antibody then is detained on first milipore filter, then arrange into the centrifugal inner tube through the catheter in, then carry out centrifugal concentration to antibody solution once more to concentration efficiency has been improved, make concentration effect better.

(2) The utility model discloses concentrated whole automation mechanized operation need not personnel and carries out manual cooperation, and work efficiency is high, and concentrated fast, time end to whole small, it is comparatively convenient to use, and the antibody solution after the concentration can directly take out convenient to use.

Drawings

Fig. 1 is a schematic view of the internal structure of the present invention;

FIG. 2 is an external view of the present invention;

fig. 3 is a schematic structural view of the centrifugal inner cylinder of the present invention.

The reference numbers in the figures are: 1. a tank body; 2. a base; 3. a liquid inlet; 4. a first ultrafiltration membrane; 5. a catheter; 6. a first valve; 7. a waste liquid guide plate; 8. a first row of waste pipes; 9. a second valve; 10. a liquid guide plate; 11. a liquid guide port; 12. a centrifugal outer cylinder; 13. centrifuging the inner barrel; 14. a drive shaft; 15. a bearing; 16. a driven gear; 17. a centrifugal motor; 18. a driving gear; 19. a toothed chain; 20. a fixing ring; 21. a protective cover; 22. a second waste pipe; 23. a fixing plate; 24. a waste discharge port; 25. a second ultrafiltration membrane; 26. a material taking port; 27. a waterproof cover; 28. a transparent glazing.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1-3, the utility model provides a technical solution: an antibody concentration device comprises a tank body 1 and a toothed chain 19, wherein a base 2 is installed at the bottom end of the tank body 1, a liquid inlet 3 is formed in the middle position of the top end of the tank body 1, a first ultrafiltration membrane 4 is arranged at the upper end inside the tank body 1, a liquid guide tube 5 is installed at one end of the first ultrafiltration membrane 4, the other end of the liquid guide tube 5 is inserted into the tank body 1, a waste liquid guide plate 7 is arranged right below the first ultrafiltration membrane 4, a first waste discharge tube 8 is installed on the tank body 1 at the lower end of the waste liquid guide plate 7, a first valve 6 and a second valve 9 are respectively installed on the liquid guide tube 5 and the first waste discharge tube 8, a liquid guide plate 10 is installed right below the waste liquid guide plate 7, a liquid guide port 11 is formed in the middle position of the liquid guide plate 10, a bottom pipe port of the liquid guide tube 5 is located right above the liquid guide port 11, a centrifugal outer cylinder 12 is arranged below the liquid, the bottom of the centrifugal outer cylinder 12 is provided with a transmission shaft 14, the middle of the transmission shaft 14 is provided with a bearing 15, the bottom of the tank body 1 is provided with a fixing plate 23, the bearing 15 is integrally embedded on the fixing plate 23, the bottom of the transmission shaft 14 is provided with a driven gear 16, one side of the tank body 1 is welded with a fixing ring 20, the inside of the fixing ring 20 is sleeved with a centrifugal motor 17, a bottom rotating shaft end of the centrifugal motor 17 is provided with a driving gear 18, the driving gear 18 is in transmission connection with the driven gear 16 through a toothed chain 19, one side of the tank body 1 is just opposite to the position of the centrifugal outer cylinder 12 and is provided with a material taking port 26, the upper end of the material taking port 26 is movably hinged with a waterproof cover 27, the middle of the waterproof cover 27 is provided with a transparent glass window 28, the side surface of the centrifugal inner cylinder 13 is provided with a plurality of second ultrafiltration.

When in use, firstly, the antibody solution to be concentrated is poured into the tank body 1 through the liquid inlet 3, the solution is filtered through the first ultrafiltration membrane 4, the reagent and the small molecular water in the solution fall onto the waste liquid guide plate 7 through the first ultrafiltration membrane 4 and are discharged through the first waste discharge pipe 8, the antibody is retained on the first ultrafiltration membrane 4 and then is discharged into the centrifugal inner cylinder 13 through the liquid guide pipe 5, at the moment, the centrifugal motor 17 is started to drive the driving gear 18 to rotate, the driving gear 18 is in transmission connection with the driven gear 16 through the toothed chain 19, the centrifugal outer cylinder 12 and the centrifugal inner cylinder 13 are driven by the transmission shaft 14 to drive the primarily concentrated antibody solution to rotate at a high speed, the side surface of the centrifugal inner cylinder 13 is provided with a plurality of second ultrafiltration membranes 25, the reagent and the small molecular water in the antibody solution can be rapidly thrown onto the inner wall of the centrifugal outer cylinder 12 through the second ultrafiltration membranes 25, and (3) discharging the waste into the tank body 1 from the waste discharge opening 24 along the inner wall of the centrifugal outer cylinder 12, then discharging the waste through the second waste discharge pipe 22 at the bottom of the tank body 1, opening the waterproof cover 27 after the centrifugation is finished, taking the centrifugal outer cylinder 12 out of the centrifugal outer cylinder 13, and pouring out and collecting the waste.

To sum up can, the utility model discloses a set up first milipore filter 4, centrifugal urceolus 12 and centrifugal inner tube 13, solved the concentrated cycle length of current technique, complex operation, the poor problem of concentrated effect.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An antibody enrichment facility, includes jar body (1) and toothed chain (19), its characterized in that: the utility model discloses a waste liquid treatment tank, including a tank body (1), a base (2) is installed to the bottom of the tank body (1), inlet (3) have been seted up to the top intermediate position of the tank body (1), the inside upper end of the tank body (1) is provided with first milipore filter (4), catheter (5) are installed to the one end of first milipore filter (4), the other end of catheter (5) inserts inside the tank body (1), be provided with waste liquid guide plate (7) under first milipore filter (4), be located install first row of waste pipe (8) on the lower one end tank body (1) of waste liquid guide plate (7), install first valve (6) and second valve (9) on catheter (5) and first row of waste pipe (8) respectively, install drain guide plate (10) under waste liquid guide plate (7), drain mouth (11) have been seted up to the positive intermediate position of drain guide plate (10), the bottom tube opening of the liquid guide tube (5) is positioned right above the liquid guide opening (11), a centrifugal outer cylinder (12) is arranged in the tank body (1) below the liquid guide plate (10), a centrifugal inner cylinder (13) is arranged in the centrifugal outer cylinder (12), a transmission shaft (14) is arranged at the bottom end of the centrifugal outer cylinder (12), a bearing (15) is arranged in the middle of the transmission shaft (14), a fixing plate (23) is arranged at the bottom of the tank body (1), the bearing (15) is integrally embedded on the fixing plate (23), the bottom end of the transmission shaft (14) is provided with a driven gear (16), one side of the tank body (1) is welded with a fixed ring (20), the interior of the fixed ring (20) is sleeved with a centrifugal motor (17), a driving gear (18) is arranged at the bottom rotating shaft end of the centrifugal motor (17), the driving gear (18) is in transmission connection with the driven gear (16) through a toothed chain (19).

2. An antibody concentration device according to claim 1, wherein: one side of the tank body (1) is just opposite to the position of the centrifugal outer cylinder (12) and is provided with a material taking port (26), the upper end of the material taking port (26) is movably hinged with a waterproof cover (27), and a transparent glass window (28) is arranged in the middle of the waterproof cover (27).

3. An antibody concentration device according to claim 1, wherein: and a plurality of second ultrafiltration membranes (25) are arranged on the side surface of the centrifugal inner cylinder (13).

4. An antibody concentration device according to claim 1, wherein: the upper end of the bearing (15) is provided with a protective cover (21).

5. An antibody concentration device according to claim 1, wherein: and a second waste discharge pipe (22) is arranged at the bottom end of the fixing plate (23).

6. An antibody concentration device according to claim 1, wherein: the bottom of the side surface of the centrifugal outer cylinder (12) is provided with a waste discharge port (24).