CN209292266U - A kind of purification and recovery device of antibody - Google Patents
A kind of purification and recovery device of antibody Download PDFInfo
- Publication number
- CN209292266U CN209292266U CN201821761180.5U CN201821761180U CN209292266U CN 209292266 U CN209292266 U CN 209292266U CN 201821761180 U CN201821761180 U CN 201821761180U CN 209292266 U CN209292266 U CN 209292266U
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- China
- Prior art keywords
- purification
- fixedly installed
- antibody
- recovery device
- blowing cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000746 purification Methods 0.000 title claims abstract description 21
- 238000011084 recovery Methods 0.000 title claims abstract description 17
- 238000007664 blowing Methods 0.000 claims abstract description 27
- 238000009833 condensation Methods 0.000 claims abstract description 20
- 230000005494 condensation Effects 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 241000883990 Flabellum Species 0.000 claims description 15
- 230000005540 biological transmission Effects 0.000 claims description 11
- 229920001971 elastomer Polymers 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 8
- 239000012153 distilled water Substances 0.000 abstract description 7
- 210000002966 serum Anatomy 0.000 abstract description 7
- 239000000243 solution Substances 0.000 abstract description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract description 4
- 238000011091 antibody purification Methods 0.000 abstract description 4
- 238000004090 dissolution Methods 0.000 abstract description 4
- 230000001376 precipitating effect Effects 0.000 abstract description 4
- 238000001556 precipitation Methods 0.000 abstract description 4
- 239000002504 physiological saline solution Substances 0.000 abstract description 3
- 238000004064 recycling Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 241001075561 Fioria Species 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to technical field of biotechnology, and disclose a kind of purification and recovery device of antibody, including mounting seat, the load-bearing pillar that quantity is two is fixedly installed at the top of the mounting seat, the driving motor being fixedly installed between two load-bearing pillars at the top of the mounting seat, the top of two load-bearing pillars are fixedly connected with the bottom of blowing cylinder.The purification and recovery device of the antibody, the serum magma purified needed for being placed by the blowing cylinder of setting, suitable distilled water is added in expansion chamber, then suitable physiological saline is added, suitable saturated ammonium sulfate is added, so that precipitating dissolution, pass through the thin filter plate part albumen precipitation of setting, it is entered in condenser pipe by the delivery line of setting, it is condensed by setting the indoor condensate liquid of condensation, by go out liquid faucet can mobile phone high concentration antibody extracting solution, improve the efficiency of antibody purification recycling, it reduces costs, experimenter is facilitated to use.
Description
Technical field
The utility model relates to technical field of biotechnology, the purification and recovery device of specially a kind of antibody.
Background technique
Antibody is a kind of special protein molecule, by the drug of reagent, treatment disease as in-vitro diagnosis, immune parent
It with the aglucon of chromatography etc., has a wide range of applications in life science, biotechnology and medical domain, especially antibody is made
For the core reagent of various immunoassays, the sensitivity and specificity of immunoassay results are played a crucial role, are made
The antibody of standby high specific and high-titer is the basis of immunological technique, but no matter is required using the antibody of which kind of technology of preparing
It is purified, the purifies and separates of antibody are primarily referred to as from the clear middle separation purpose antibody of immune zoology.
It the use of wide technology include at present column chromatography and ammonium sulfate precipitation etc., it is pure that technology has chromatographic column
Change the good advantage of specificity, but device and cumbersome, it is difficult to it carries out the high-throughput of research and development property scale and rapidly purifies, sulfuric acid ammonia-sinking
Shallow lake purification specificity is poor, and purification process is cumbersome and time-consuming, and is further purified without recyclable device to antibody, to reach
To perfect condition, so a kind of purification and recovery device of antibody is proposed to solve the above problems.
Utility model content
(1) the technical issues of solving
In view of the deficiencies of the prior art, the utility model provides a kind of purification and recovery device of antibody, has efficiently pure
The advantages that change, solves current device and cumbersome, it is difficult to and it carries out the high-throughput of research and development property scale and rapidly purifies, sulfuric acid ammonia-sinking
Shallow lake purification specificity is poor, and purification process is cumbersome and time-consuming, and is further purified without recyclable device to antibody, to reach
The problem of to perfect condition.
(2) technical solution
For the purpose for realizing above-mentioned efficiently purifying, the utility model is provided the following technical solutions: a kind of purifying of antibody time
Receiving apparatus, including mounting seat are fixedly installed with the load-bearing pillar that quantity is two, the installation bottom at the top of the mounting seat
The top of seat is fixedly installed with the driving motor between two load-bearing pillars, the top of two load-bearing pillars with blowing cylinder
Bottom be fixedly connected, the output shaft of the driving motor is fixedly connected with the transmission shaft through blowing cylinder, the transmission shaft
Top is fixedly installed with flabellum, and the inner bottom wall of the blowing cylinder is fixedly installed with the filter screen outside flabellum, the blowing
The bottom contact of cylinder has top cover, and handle, the fixed peace in the left and right sides of the blowing cylinder are fixedly installed at the top of the top cover
Equipped with the fixture block being clamped with top cover, it is communicated with outlet tube on the right side of the blowing cylinder, control faucet is installed on the outlet tube,
The right end of the outlet tube is communicated with expansion chamber, and the inside of the expansion chamber is fixedly installed with thin filter plate, the expansion chamber
Right side is communicated with delivery line, and the right end of the delivery line is communicated with condenser pipe, and the right end of the condenser pipe is fixedly connected with outlet
Pipe, the outside of the condenser pipe is fixedly installed with condensation chamber, fixed frame, the fixation is fixedly installed on the left of the condensation chamber
The display tube extended inside condensation chamber is fixedly installed on frame, the bottom of the condenser pipe, which is communicated with, to be extended to outside condensation chamber
Collecting pipe, the bottom of the collecting pipe are communicated with out liquid faucet.
Preferably, top cover left and right sides wall opens up fluted, and two fixture blocks are respectively engaged with two grooves
It is interior.
Preferably, the diameter of the filter screen is adapted with the internal diameter of blowing cylinder, and between existing between filter screen and flabellum
Gap.
Preferably, the condenser pipe is distributed in a ring, and the junction of the condenser pipe and delivery line is equipped with rubber ring.
Preferably, the inside of the condensation chamber is filled with condensate liquid.
Preferably, the display tube is L-shaped, and the top length of the display tube is less than its bottom lengths.
(3) beneficial effect
Compared with prior art, the utility model provides a kind of purification and recovery device of antibody, has following beneficial to effect
Fruit:
The purification and recovery device of the antibody, the serum magma purified needed for being placed by the blowing cylinder of setting are added certain
The distilled water of ratio is driven by the driving motor of setting, so that transmission shaft drives flabellum rotation, so that flabellum is to serum
Magma carries out high-speed stirred centrifugation, is centrifuged the magma finished and falls thick impurity by filter screen filtration, doped with the virgin pulp liquid of antibody
Body is flowed into expansion chamber by the outlet tube being arranged, and suitable distilled water is added in expansion chamber, then adds suitable life
Salt water is managed, suitable saturated ammonium sulfate is added, so that precipitating dissolution, by the thin filter plate part albumen precipitation of setting,
It is entered in condenser pipe, it is condensed by setting the indoor condensate liquid of condensation, by going out liquid dragon by the delivery line of setting
Head can mobile phone high concentration antibody extracting solution, improve antibody purification recycling efficiency, reduce costs, facilitate experimenter
It uses.
Detailed description of the invention
FIG. 1 is a schematic structural view of the utility model；
Fig. 2 is the enlarged drawing in the utility model Fig. 1 at A；
Fig. 3 is the enlarged drawing in the utility model Fig. 1 at B.
In figure: 1 mounting seat, 2 load-bearing pillars, 3 driving motors, 4 blowing cylinders, 5 transmission shafts, 6 flabellums, 7 filter screens, 8 tops
Lid, 9 handles, 10 fixture blocks, 11 outlet tubes, 12 control faucets, 13 expansion chambers, 14 thin filter plates, 15 delivery lines, 16 condenser pipes, 17
Escape pipe, 18 condensation chambers, 19 fixed frames, 20 display tubes, 21 collecting pipes, 22 go out liquid faucet.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-3, a kind of purification and recovery device of antibody, including mounting seat 1, the top of mounting seat 1 is please referred to fix
The load-bearing pillar 2 that quantity is two is installed, the top of mounting seat 1 is fixedly installed with the transmission between two load-bearing pillars 2
Motor 3, the model of driving motor 3 can be TCB8014B3, and the top of two load-bearing pillars 2, which is fixed with the bottom of blowing cylinder 4, to be connected
It connects, the output shaft of driving motor 3 is fixedly connected with the transmission shaft 5 through blowing cylinder 4, and the top of transmission shaft 5 is fixedly installed with fan
Leaf 6, the inner bottom wall of blowing cylinder 4 are fixedly installed with the filter screen 7 outside flabellum 6, the diameter and blowing cylinder 4 of filter screen 7
Internal diameter be adapted, and there are gap between filter screen 7 and flabellum 6, the bottom contact of blowing cylinder 4 has top cover 8, the top of top cover 8
Portion is fixedly installed with handle 9, and the left and right sides of blowing cylinder 4 is fixedly installed with the fixture block 10 being clamped with top cover 8, top cover 8 or so
Two sidewalls open up fluted, and two fixture blocks 10 are respectively engaged in two grooves, and the right side of blowing cylinder 4 is communicated with outlet tube
11, control faucet 12 is installed, the right end of outlet tube 11 is communicated with expansion chamber 13, and the inside of expansion chamber 13 is fixed on outlet tube 11
Thin filter plate 14 is installed, the right side of expansion chamber 13 is communicated with delivery line 15, and the right end of delivery line 15 is communicated with condenser pipe 16, cold
Solidifying pipe 16 is distributed in a ring, and the junction of condenser pipe 16 and delivery line 15 is equipped with rubber ring, and the right end of condenser pipe 16 is fixed to be connected
It is connected to escape pipe 17, the outside of condenser pipe 16 is fixedly installed with condensation chamber 18, and the inside of condensation chamber 18 is filled with condensate liquid, condensation
The left side of room 18 is fixedly installed with fixed frame 19, and the display tube 20 extended inside condensation chamber 18 is fixedly installed on fixed frame 19,
Display tube 20 is L-shaped, and the top length of display tube 20 is less than its bottom lengths, and the bottom of condenser pipe 16, which is communicated with, to be extended to
Collecting pipe 21 outside condensation chamber 18, the bottom of collecting pipe 21 are communicated with out liquid faucet 22, place institute by the blowing cylinder 4 of setting
The serum magma that need to be purified, is added a certain proportion of distilled water, is driven by the driving motor 3 of setting, so that transmission shaft 5 drives
Flabellum 6 rotates, so that flabellum 6 carries out high-speed stirred centrifugation to serum magma, is centrifuged the magma finished and passes through 7 mistake of filter screen
Thick impurity is filtered, is flowed into expansion chamber 13 doped with the magma liquid of antibody by the outlet tube 11 of setting, in expansion chamber 13
It is interior that suitable distilled water is added, suitable physiological saline is then added, suitable saturated ammonium sulfate is added, so that precipitating dissolution,
By the 14 filtration fraction albumen precipitation of thin filter plate of setting, is entered in condenser pipe 16, passed through by the delivery line 15 of setting
If the condensate liquid in condensation chamber 18 condenses it, by go out liquid faucet 22 can mobile phone high concentration antibody extracting solution, mention
The efficiency for having risen antibody purification recycling, reduces costs, experimenter is facilitated to use.
In conclusion the purification and recovery device of the antibody, the serum purified needed for being placed by the blowing cylinder 4 of setting is former
Slurry, is added a certain proportion of distilled water, is driven by the driving motor 3 of setting, so that transmission shaft 5 drives flabellum 6 to rotate, thus
So that flabellum 6 carries out high-speed stirred centrifugation to serum magma, it is centrifuged the magma finished and thick impurity is filtered out by filter screen 7, mix
The miscellaneous magma liquid for having antibody is flowed into expansion chamber 13 by the outlet tube 11 of setting, and suitable steaming is added in expansion chamber 13
Then distilled water adds suitable physiological saline, suitable saturated ammonium sulfate is added, so that precipitating dissolution, passes through the thin mistake of setting
14 filtration fraction albumen precipitation of filter plate, is entered in condenser pipe 16 by the delivery line 15 of setting, by setting in condensation chamber 18
Condensate liquid condenses it, by go out liquid faucet 22 can mobile phone high concentration antibody extracting solution, improve antibody purification return
The efficiency of receipts, reduces costs, and experimenter is facilitated to use.
It should be noted that the terms "include", "comprise" or its any other variant are intended to the packet of nonexcludability
Contain, so that the process, method, article or equipment for including a series of elements not only includes those elements, but also including
Other elements that are not explicitly listed, or further include for elements inherent to such a process, method, article, or device.
In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that including the element
Process, method, article or equipment in there is also other identical elements.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of purification and recovery device of antibody, including mounting seat (1), it is characterised in that: the top of the mounting seat (1)
It is fixedly installed with the load-bearing pillar (2) that quantity is two, is fixedly installed at the top of the mounting seat (1) positioned at two load-bearing pillars
(2) top of the driving motor (3) between, two load-bearing pillars (2) is fixedly connected with the bottom of blowing cylinder (4), described
The output shaft of driving motor (3) is fixedly connected with the transmission shaft (5) through blowing cylinder (4), and the top of the transmission shaft (5) is fixed
It is equipped with flabellum (6), the inner bottom wall of the blowing cylinder (4) is fixedly installed with the filter screen (7) external positioned at flabellum (6), described
The bottom contact of blowing cylinder (4) has top cover (8), is fixedly installed with handle (9) at the top of the top cover (8), the blowing cylinder (4)
The left and right sides be fixedly installed with the fixture block (10) with top cover (8) clamping, be communicated with outlet tube on the right side of the blowing cylinder (4)
(11), control being installed leading (12) on the outlet tube (11), the right end of the outlet tube (11) is communicated with expansion chamber (13),
The inside of the expansion chamber (13) is fixedly installed with thin filter plate (14), is communicated with delivery line on the right side of the expansion chamber (13)
(15), the right end of the delivery line (15) is communicated with condenser pipe (16), and the right end of the condenser pipe (16) is fixedly connected with outlet
It manages (17), the outside of the condenser pipe (16) is fixedly installed with condensation chamber (18), and the left side of the condensation chamber (18) is fixedly mounted
Have fixed frame (19), is fixedly installed on the fixed frame (19) and extends condensation chamber (18) internal display tube (20), it is described cold
The bottom of solidifying pipe (16), which is communicated with, extends to the external collecting pipe (21) of condensation chamber (18), the bottom connection of the collecting pipe (21)
There is liquid faucet (22) out.
2. a kind of purification and recovery device of antibody according to claim 1, it is characterised in that: top cover (8) left and right two
Side wall opens up fluted, and two fixture blocks (10) are respectively engaged in two grooves.
3. a kind of purification and recovery device of antibody according to claim 1, it is characterised in that: the filter screen (7) it is straight
Diameter is adapted with the internal diameter of blowing cylinder (4), and there are gaps between filter screen (7) and flabellum (6).
4. a kind of purification and recovery device of antibody according to claim 1, it is characterised in that: the condenser pipe (16) is in ring
Shape distribution, the condenser pipe (16) and the junction of delivery line (15) are equipped with rubber ring.
5. a kind of purification and recovery device of antibody according to claim 1, it is characterised in that: the condensation chamber (18) it is interior
Portion is filled with condensate liquid.
6. a kind of purification and recovery device of antibody according to claim 1, it is characterised in that: the display tube (20) is in
The top length of L shape, the display tube (20) is less than its bottom lengths.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821761180.5U CN209292266U (en) | 2018-10-29 | 2018-10-29 | A kind of purification and recovery device of antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821761180.5U CN209292266U (en) | 2018-10-29 | 2018-10-29 | A kind of purification and recovery device of antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209292266U true CN209292266U (en) | 2019-08-23 |
Family
ID=67647079
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821761180.5U Expired - Fee Related CN209292266U (en) | 2018-10-29 | 2018-10-29 | A kind of purification and recovery device of antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209292266U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111138525A (en) * | 2019-12-24 | 2020-05-12 | 天津奇特尔生物科技有限公司 | Antibody purification and recovery device and operation method |
| CN112480251A (en) * | 2020-11-30 | 2021-03-12 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN113185606A (en) * | 2021-05-19 | 2021-07-30 | 青岛瑞斯凯尔生物科技有限公司 | Purification device for antibody and working method thereof |
-
2018
- 2018-10-29 CN CN201821761180.5U patent/CN209292266U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111138525A (en) * | 2019-12-24 | 2020-05-12 | 天津奇特尔生物科技有限公司 | Antibody purification and recovery device and operation method |
| CN111138525B (en) * | 2019-12-24 | 2023-04-25 | 天津奇特尔生物科技有限公司 | Antibody purification and recovery device and operation method |
| CN112480251A (en) * | 2020-11-30 | 2021-03-12 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN112480251B (en) * | 2020-11-30 | 2023-07-11 | 江西中洪博元生物技术有限公司 | Intelligent high-specificity combined VEGFA multi-antibody screening and purifying device |
| CN113185606A (en) * | 2021-05-19 | 2021-07-30 | 青岛瑞斯凯尔生物科技有限公司 | Purification device for antibody and working method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190823 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |