CN210347276U - Animal blood preparation device is used in laboratory - Google Patents
Animal blood preparation device is used in laboratory Download PDFInfo
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- CN210347276U CN210347276U CN201921196882.8U CN201921196882U CN210347276U CN 210347276 U CN210347276 U CN 210347276U CN 201921196882 U CN201921196882 U CN 201921196882U CN 210347276 U CN210347276 U CN 210347276U
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Abstract
The utility model discloses an animal blood preparation device for laboratory, which comprises a shell, wherein a driving motor is arranged in the shell, the driving motor is connected with a rotor platform, a physiological saline barrel is arranged on the upper part of the rotor platform in a matching way, a plurality of centrifugal holes are arranged on the outer ring of the rotor platform, the centrifugal holes are symmetrically arranged in two groups, two configuration holes are arranged on the inner ring of the rotor platform, the bottoms of the configuration holes are communicated with a conduit, and the conduit is provided with an on-off valve; each group of centrifugal holes are sequentially and respectively communicated at the bottom, a switch valve is arranged between every two adjacent centrifugal holes, the last centrifugal hole of each group of centrifugal holes is communicated with one of the configuration holes at the bottom, and the switch valve is also arranged at the communication position. The device can realize the full automatization preparation of a small amount of for experiments erythrocyte suspension, has saved loaded down with trivial details operation process, has reduced the time of experimenter with experimental animals blood contact relatively, ensures human factor of safety, improves work efficiency.
Description
Technical Field
The utility model relates to a medicine inspection, health products inspection, scientific research test articles for use field, concretely relates to animal blood preparation facilities is used in laboratory.
Background
Erythrocyte suspensions with various concentrations are often used as test carriers in the processes of medicine inspection, health product inspection, scientific research experiment and the like. One of the key steps in the preparation of a suspension of red blood cells is the washing of the red blood cells. At present, the red blood cell cleaning method is to add a certain amount of physiological saline into red blood cells and use a centrifuge for centrifugation to achieve the purpose of cleaning the red blood cells, and the red blood cells are usually cleaned for many times until the supernatant does not show red. The traditional mode is that a common centrifuge is used for centrifuging for a plurality of times, and the traditional mode has certain defects: 1) after each centrifugation, the centrifuge needs to be opened, the supernatant is discarded, and new physiological saline is added, so that the operation is complicated; 2) the time and the times of contacting the experimental blood of the experimenter are increased, and certain safety risks exist. At present, blood preparation devices used in blood stations and blood banks have large volume and capacity, and the volume of red blood cells required to be cleaned in the field of experimental research is small, generally 2-3 ml. Therefore, blood preparation devices for blood stations and blood banks are not suitable for laboratory use.
SUMMERY OF THE UTILITY MODEL
To above problem, the utility model provides an animal blood preparation facilities is used in laboratory, the device can realize the full automatization preparation of a small amount of for experiments erythrocyte suspension, has saved loaded down with trivial details operation process, has reduced the time of experimenter with experimental animals blood contact relatively, ensures human factor of safety, improves work efficiency.
The utility model discloses a realize through following measure:
an animal blood preparation device for a laboratory comprises a shell, wherein a driving motor is installed in the shell and connected with a rotor table, a physiological saline barrel is installed on the upper portion of the rotor table in a matched mode, a plurality of centrifugal holes are formed in the outer ring of the rotor table and are symmetrically arranged in two groups, two configuration holes are formed in the inner ring of the rotor table, the bottoms of the configuration holes are communicated with a guide pipe, and the guide pipe is provided with an on-off valve and a photometric sensor;
each group of centrifugal holes are sequentially and respectively communicated at the bottom, a switch valve and a photometric sensor are arranged between every two adjacent centrifugal holes, the last centrifugal hole of each group of centrifugal holes is communicated with one of the configuration holes at the bottom, and a switch valve is also arranged at the communicated position.
Preferably, the upper part of the physiological saline barrel is provided with a water inlet, the water inlet is provided with a sealing cover, the lower part of the physiological saline barrel is provided with a plurality of water outlets, and the positions of the water outlets respectively correspond to the positions of the centrifugal hole and the configuration hole.
Preferably, the downthehole cooperation installation centrifuging tube of centrifugation, the downthehole cooperation installation configuration pipe of configuration, centrifuging tube and configuration pipe are both the penetrating plastic tubing in both ends, the plastic tubing lower part is passed through the screw and is connected with the revolving stage.
Preferably, each water outlet is provided with a switch valve and a flow sensor.
Preferably, a partition plate is arranged in the shell, the rotor table is located above the partition plate, and the driving motor is located below the partition plate.
Preferably, an upper cover is arranged at the upper part of the shell, and an opening and closing door is arranged on one side surface of the shell.
Preferably, a display screen is arranged on the side face of the shell, and a plurality of operation keys are arranged around the display screen.
The beneficial effects of the utility model.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a perspective view of the overall structure of the present invention;
FIG. 2 is a top view of the overall structure of the present invention;
FIG. 3 is a cross-sectional view taken at C-C of FIG. 2;
FIG. 4 is a bottom view of a saline pail;
FIG. 5 is a sectional view taken along line A-A of FIG. 4;
in the figure, the device comprises a shell 1, a shell 1-1, an upper cover 1-2, a switch door 2, a physiological saline barrel 2-1, a sealing cover 2-2, a water outlet hole 3, a rotor table 4, a centrifugal tube 5, a first switch valve 6, a second switch valve 7, an on-off valve 8, a driving motor 9 and a guide tube.
Detailed Description
The technical solutions in the embodiments of the present application will be described clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
As shown in FIGS. 1 to 5, in the laboratory animal blood preparing apparatus, an upper cover 1-1 is hinge-mounted to an upper portion of a case 1 for preventing dust from entering and liquid from splashing. And the right side surface of the shell is provided with a switch door 1-2 which is used for opening the switch door after the experiment is finished and removing a conduit 9 to fill the prepared blood into a specified container. The side of casing is provided with the display screen, be provided with a plurality of operating keys around the display screen for the action of control driving motor and ooff valve and on-off valve.
Install the baffle in the casing 1, driving motor 8 installs the plane under the baffle, and rotor platform 3 installs on baffle upper portion, and rotor platform 3 passes through the key-type connection with driving motor 8, and along with operation driving motor is just reversing, drives rotor platform forward or reverse rotation.
8 centrifugal holes are machined in the circumferential direction of the outer ring of the rotor table, and a 15ml centrifugal tube 4 is placed in each centrifugal hole; two configuration holes are arranged at the middle diagonal, and a configuration tube of about 100ml is placed in each configuration hole and is used for preparing erythrocyte suspension after centrifugation. The centrifugal tube and the configuration tube are both plastic tubes with two through ends, and the lower parts of the plastic tubes are connected with the rotating table through threads. Every group centrifuging tube communicates separately in proper order through the inside passage of rotor platform in the bottom, installs the second ooff valve between every two adjacent centrifuging tubes of inside passage and is used for the luminosity sensor of sensitization, and the last centrifuging tube of every group centrifuging tube and one of them configuration pipe communicate through the inside passage of rotor platform in the bottom to also be provided with second ooff valve 6 in intercommunication department. The bottom end of the configuration pipe is communicated with a guide pipe 9, and an on-off valve 8 is arranged at the connection part of the guide pipe.
The upper part of the rotor platform 3 is provided with a physiological saline barrel 2 in a matching way, the upper part of the physiological saline barrel 2 is provided with a water inlet hole, the water inlet hole is provided with a sealing cover 2-1, the lower part of the physiological saline barrel is provided with a plurality of water outlet holes, the positions of the water outlet holes respectively correspond to the positions of the centrifugal hole and the configuration hole, and each water outlet hole is provided with a first switch valve 5 and a flow sensor.
The blood preparation using the device was carried out as follows:
1. all the switch valves and the on-off valves of the device are ensured to be in a closed state;
2. selecting a proper number of centrifuge tubes to be connected with the guide tube according to the centrifugation times required by the experiment, placing the centrifuge tubes into the centrifuge holes, and fixing the lower threads;
3. a proper amount of defibered blood (about 1.5 ml) was placed in the first centrifuge tube of each set of centrifuge tubes, respectively;
4. the physiological saline barrel and the rotor platform are installed in a matched mode, and each water outlet hole of the physiological saline barrel is ensured to be matched with the corresponding centrifugal tube and the corresponding configuration tube;
5. setting the rotation speed, the time, the centrifugation times, the concentration of the erythrocyte suspension and the volume of the defibered blood to be added through an operation interface, and pressing a start key;
6. firstly, opening a first switch valve at a physiological saline water outlet corresponding to the No. 1 centrifugal tube of each group, enabling the physiological saline water to flow into the No. 1 centrifugal tube, adding the corresponding volume of physiological saline water into a flow sensor according to the volume of the defibered blood in a volume ratio of 1:9, and then closing the first switch valve;
7. carrying out first centrifugation;
8.1 after the centrifugation of the No. 1 tube, opening a second switch valve between the No. 1 tube and the No. 2 tube, enabling lower-layer red blood cells after the centrifugation of the No. 1 centrifuge tube to flow to the No. 2 centrifuge tube, closing the second switch valve when a photometric sensor between the No. 1 centrifuge tube and the No. 2 centrifuge tube senses that no red blood cells pass through (transmittance is increased), opening a first switch valve of a physiological saline pipeline corresponding to the No. 2 centrifuge tube, controlling to release physiological saline with the same volume as the physiological saline added into the No. 1 centrifuge tube to enter the No. 2 centrifuge tube by a flow sensor, and closing the first switch valve;
9. carrying out second centrifugation;
10. the third centrifugation is analogized in turn;
11. according to a set program, after the last centrifugation is finished, a second switch valve below the last centrifugal tube is opened, the lower layer red blood cells flow to the red blood cell suspension preparation tube, the volume of the red blood cells is quantitatively released by the photometric sensor according to the concentration of the red blood cell suspension set by the operation interface, and then the second switch valve is closed;
12. a first switch valve of the erythrocyte suspension preparation tube, which is connected with the physiological saline barrel, is opened, and according to the set concentration, the flow sensor quantitatively adds the volume of the physiological saline and closes the first switch valve;
13. switching the rotating speed of a motor, and vibrating the erythrocyte suspension preparation tube to fully mix the erythrocyte suspension; (the procedure can rotate the centrifugal rotor carrying the erythrocyte suspension preparation tube back and forth left and right to achieve the effect of mixing the suspension in the preparation tube, and the procedure needs to shake and mix the suspension once every 30 seconds until the operation interface is finished by pressing a stop key, and the procedure needs to be carried out when the upper cover is opened)
14. And opening the upper cover, connecting a conduit below the erythrocyte suspension preparation tube with a corresponding container, opening a second switch valve, and respectively releasing the erythrocyte suspension into the required experiment tube according to the set liquid separation volume.
15. After all the operations are finished, pressing a cleaning key on an operation interface, opening all switch valves and on-off valves, enabling physiological saline to flow through all the passages, enabling the preparation tube to still have a vibration function to achieve the cleaning purpose, placing the catheter head into a waste liquid pool, and cleaning the pipeline for 1-2min, so that all supernatant liquid on the centrifugal upper layer in the pipeline and waste liquid such as residual erythrocyte suspension in the pipeline flow out.
16. Evacuation operation: closing a first switch valve connected with a physiological saline barrel, opening a second switch valve and an on-off valve, and emptying all liquid in a pipeline to a waste liquid pool;
17. and pressing a stop key to finish the test and turning off the power supply.
18. The pipeline can be disassembled and then cleaned according to other laboratory requirements.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. An animal blood preparation device for a laboratory is characterized by comprising a shell, wherein a driving motor is installed in the shell and connected with a rotor table, a physiological saline barrel is installed on the upper portion of the rotor table in a matched mode, a plurality of centrifugal holes are formed in the outer ring of the rotor table and are symmetrically arranged in two groups, two configuration holes are formed in the inner ring of the rotor table, a guide pipe is communicated with the bottom of each configuration hole, and the guide pipe is provided with an on-off valve and a photometric sensor;
each group of centrifugal holes are sequentially and respectively communicated at the bottom, a switch valve and a photometric sensor are arranged between every two adjacent centrifugal holes, the last centrifugal hole of each group of centrifugal holes is communicated with one of the configuration holes at the bottom, and a switch valve is also arranged at the communicated position.
2. The animal blood preparation device for the laboratory according to claim 1, wherein a water inlet is provided at an upper portion of the physiological saline barrel, a sealing cover is provided at the water inlet, a plurality of water outlet holes are provided at a lower portion of the physiological saline barrel, and the positions of the water outlet holes correspond to the positions of the centrifugal hole and the configuration hole respectively.
3. The animal blood preparation device for laboratories according to claim 2, wherein a centrifuge tube is fittingly installed in the centrifuge hole, a configuration tube is fittingly installed in the configuration hole, the centrifuge tube and the configuration tube are both plastic tubes with two through ends, and the lower portion of the plastic tube is connected with the rotary table through a thread.
4. The laboratory animal blood preparation device according to claim 3, wherein each outlet is provided with a switch valve and a flow sensor.
5. The laboratory animal blood preparation device of claim 1, wherein a partition is disposed within the housing, the rotor stage is positioned above the partition, and the drive motor is positioned below the partition.
6. The laboratory animal blood preparation device according to claim 5, wherein an upper cover is provided on the upper portion of the housing, and an opening and closing door is provided on one side of the housing.
7. The laboratory animal blood preparation device according to claim 5, wherein a display screen is disposed on a side surface of the housing, and a plurality of operation keys are disposed around the display screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921196882.8U CN210347276U (en) | 2019-07-29 | 2019-07-29 | Animal blood preparation device is used in laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921196882.8U CN210347276U (en) | 2019-07-29 | 2019-07-29 | Animal blood preparation device is used in laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210347276U true CN210347276U (en) | 2020-04-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921196882.8U Expired - Fee Related CN210347276U (en) | 2019-07-29 | 2019-07-29 | Animal blood preparation device is used in laboratory |
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| CN (1) | CN210347276U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323837A (en) * | 2021-12-02 | 2022-04-12 | 湖北文理学院 | Automatic manufacturing device and manufacturing method for erythrocyte suspension |
-
2019
- 2019-07-29 CN CN201921196882.8U patent/CN210347276U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114323837A (en) * | 2021-12-02 | 2022-04-12 | 湖北文理学院 | Automatic manufacturing device and manufacturing method for erythrocyte suspension |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200417 Termination date: 20210729 |
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| CF01 | Termination of patent right due to non-payment of annual fee |