CN211896951U

CN211896951U - Purification device for nucleic acid extraction

Info

Publication number: CN211896951U
Application number: CN202020288137.2U
Authority: CN
Inventors: 夏国庆; 郝若尘
Original assignee: Changzhou Baidai Biotechnology Co ltd
Current assignee: Changzhou Baidai Biotechnology Co ltd
Priority date: 2020-03-10
Filing date: 2020-03-10
Publication date: 2020-11-10
Anticipated expiration: 2030-03-10

Abstract

The utility model discloses a purification device of nucleic acid extraction usefulness belongs to molecular biology technical field. The centrifugal box comprises a box body, a top cover, a rotary table and a centrifugal tube, wherein a rotary shaft is fixedly mounted behind the upper surface of the box body, a placement groove is formed in the upper surface of the box body, a motor is fixedly mounted inside the box body, a rotor of the motor is fixedly connected with a connecting shaft, the box body is rotatably connected with the top cover through the rotary shaft, a frame groove is formed in the lower surface of the top cover, and a fixing groove is formed in the middle of the frame; set up the motor and drive the carousel and rotate, joint centrifuge tube in the carousel, place the cell turbid liquid that mixes that has cell membrane to destroy the reagent in the centrifuge tube, the centrifuge tube is rotatory constantly to get rid of intraductal mixed solution to nucleic acid filtration membrane under the drive of motor, and nucleic acid can pass nucleic acid filtration membrane to this separates out nucleic acid from cell impurity mixed liquid, improves nucleic acid separation speed, reduces the nucleic acid separation step.

Description

Purification device for nucleic acid extraction

Technical Field

The utility model belongs to the technical field of molecular biology, particularly, relate to a purification device of nucleic acid extraction usefulness.

Background

In molecular biology-related experiments, separation and purification work is very important and indispensable. However, it is still difficult to purify a plurality of samples simultaneously, and not only a suitable purification method needs to be selected, but also the workload is very large, and it is difficult to meet the requirement of extracting and purifying high-throughput samples in the current rapid development. The magnetic bead automatic nucleic acid extracting and purifying system is based on magnetic bead technology and may be used widely in genomics, disease control medicine, food safety, forensic identification and other fields.

At present, the prior art for extracting and purifying nucleic acid has the following defects:

1. in the process of extracting and purifying nucleic acid, chemical reagents are required to be added to destroy cell walls and then separate and precipitate the nucleic acid, so that the whole process is complex, the steps are complex, and the operation is inconvenient;

2. the separation process of nucleic acid is slow, and the purification effect is greatly influenced by the operation method of operators, and the extraction purity is not high.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a purification device of nucleic acid extraction usefulness possesses convenient operation, the high advantage of purity, has solved the problem at the end of inconvenient, the purity of operation.

For the purpose that realizes above-mentioned convenient operation, purity are high, the utility model provides a following technical scheme: a purification device for extracting nucleic acid comprises a box body, a top cover, a rotary table and a centrifugal tube, wherein a rotary shaft is fixedly arranged behind the upper surface of the box body;

the lower surface of the top cover is provided with a frame groove, and the middle of the frame groove is provided with a fixing groove;

a turntable is fixedly mounted at the upper end of the connecting shaft, a counterweight ring is fixedly mounted at the outer edge of the turntable, and a limit groove is formed in the upper surface of the counterweight ring;

the inside joint of spacing groove has the centrifuging tube, the right-hand member threaded connection of centrifuging tube has the pipe cap, the left end threaded connection of centrifuging tube has branch liquid pipe, the inside fixed mounting who divides liquid pipe has nucleic acid filtration membrane, the one end surface that divides liquid pipe to be close to the centrifuging tube covers there is the rubber slipmat.

As the utility model discloses an optimized technical scheme, the quantity of resettlement groove is two, two the resettlement inslot portion all rotates and is connected with a carousel, and two resettlement inslot carousel tops all prevent to have a carousel and the relative placement of same resettlement inslot's carousel spacing groove.

As an optimal technical scheme of the utility model, the both ends of centrifuging tube are all nested inside branch liquid pipe and tube cap, centrifuging tube and branch liquid pipe internal diameter equal.

As an optimal technical scheme of the utility model, when the top cap is closed on the box upper surface, the fixed slot joint is in the carousel outer fringe of corresponding arrangement inslot top.

As an optimized technical scheme of the utility model, the diameter of pipe cap is greater than the centrifuging tube diameter.

As an optimal technical scheme of the utility model, the one end cross-sectional diameter that the spacing groove is close to the arrangement groove centre of a circle is greater than the one end cross-sectional diameter that the arrangement groove kept away from the arrangement groove centre of a circle, spacing groove shape size and centrifuging tube and pipe cap shape size looks adaptation.

Compared with the prior art, the utility model provides a purification device of nucleic acid extraction usefulness possesses following beneficial effect:

(1) this purification device of nucleic acid extraction usefulness drives the carousel through setting up the motor and rotates, and joint centrifuge tube in the carousel places the cell turbid liquid that mixes that has the cell membrane to destroy reagent in the centrifuge tube, and the centrifuge tube is rotatory constantly to get rid of intraductal mixed solution to nucleic acid filtration membrane under the drive of motor, and nucleic acid can pass nucleic acid filtration membrane to this separates out nucleic acid from cell impurity mixed liquid, improves nucleic acid separation speed, reduces the nucleic acid separation step, thereby reaches the purpose of convenient operation.

(2) This purification device of nucleic acid extraction usefulness drives the centrifuging tube through setting up the carousel and rotates, and the nucleic acid in the intraductal solution of continuous giving is to the effort that nucleic acid filtration membrane removed fully filters the solution, has reduced the accounting residue in the waste material among the traditional purification process and the waste material content in the finished product to reach the mesh of improving the purity.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a cross-sectional view of the bottom case of the present invention;

fig. 3 is a top view of the turntable of the present invention;

fig. 4 is a side sectional view of the turntable of the present invention;

fig. 5 is a front cross-sectional view of the top cover of the present invention;

FIG. 6 is a schematic view of the structure of the centrifugal tube of the present invention;

figure 7 is the utility model discloses a centrifuging tube cross-sectional view.

In the figure: 1. a box body; 11. a rotating shaft; 12. a placing groove; 13. a motor; 14. a connecting shaft; 2. a top cover; 21. a frame groove; 22. fixing grooves; 3. a turntable; 31. a counterweight ring; 32. a limiting groove; 4. centrifuging the tube; 41. a liquid separating pipe; 42. a rubber non-slip mat; 43. a pipe cap; 44. a nucleic acid filtration membrane.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-7, the utility model discloses a purification device for nucleic acid extraction, including box 1, top cap 2, carousel 3 and centrifuging tube 4, the upper surface rear of box 1 is fixedly installed with pivot 11, the upper surface of box 1 has seted up arrangement groove 12, the inside fixed mounting of box 1 has motor 13, the rotor fixed connection of motor 13 has connecting axle 14, box 1 is connected with top cap 2 through pivot 11 rotation;

a frame groove 21 is formed in the lower surface of the top cover 2, and a fixing groove 22 is formed in the middle of the frame groove 21;

the upper end of the connecting shaft 14 is fixedly provided with a turntable 3, the outer edge of the turntable 3 is fixedly provided with a counterweight ring 31, and the upper surface of the counterweight ring 31 is provided with a limit groove 32;

the inside joint of spacing groove 32 has centrifuging tube 4, the right-hand member threaded connection of centrifuging tube 4 has pipe cap 43, the left end threaded connection of centrifuging tube 4 has branch liquid pipe 41, the inside fixed mounting who divides liquid pipe 41 has nucleic acid filtration membrane 44, the one end surface that divides liquid pipe 41 to be close to centrifuging tube 4 covers there is rubber slipmat 42.

Specifically, the number of the placing grooves 12 is two, two the inside of the placing grooves 12 is connected with a rotary table 3 in a rotating way, and the rotary table 3 limiting grooves 32 in the same placing groove 12 are placed above the rotary tables 3 in the two placing grooves 12 in a relative mode.

In this embodiment, two carousel 3 are placed relatively, fix centrifuge tube 4 on carousel 3, drive carousel 3 through motor 13 and rotate to drive centrifuge tube 4 and rotate.

Specifically, both ends of the centrifugal tube 4 are nested inside the liquid separating tube 41 and the tube cap 43, and the inner diameters of the centrifugal tube 4 and the liquid separating tube 41 are equal.

In this embodiment, the difference in the inner diameters of the centrifugal tube 4 and the liquid separation tube 41 may cause problems such as insufficient nucleic acid separation.

Specifically, when the top cover 2 is closed on the upper surface of the box body 1, the fixing groove 22 is clamped at the outer edge of the rotary table 3 above the corresponding placing groove 12.

In this embodiment, the fixing groove 22 on the top cover 2 is used to fix the rotating disk 3 covering the centrifuge tube 4.

Specifically, the diameter of the cap 43 is larger than that of the centrifuge tube 4.

In this embodiment, play the fixed action through tube cap 43 and 4 diameter differences of centrifuging tube to centrifuging tube 4, place carousel 3 and deviate from when rotating centrifuging tube 4.

Specifically, the diameter of the cross section of one end of the limiting groove 32 close to the circle center of the placing groove 12 is larger than the diameter of the cross section of one end of the placing groove 12 far away from the circle center of the placing groove 12, and the shape and the size of the limiting groove 32 are matched with those of the centrifugal tube 4 and the tube cap 43.

In this embodiment, the diameter change of the cross section of the limiting groove 32 is the same as the diameter change of the centrifugal tube 4 to the tube cap 43, so as to fix the centrifugal tube 4.

The utility model discloses a theory of operation and use flow: when using, 4 one ends of centrifugal tube and pipe cap 43 threaded connection, pour into the cell solution that mixes the nucleic acid and draw reagent into centrifugal tube 4, the other end and the branch liquid pipe 41 threaded connection of centrifugal tube 4, the carousel 3 on settling tank 12 of 4 joint of centrifugal tube, centrifuge tube 4 arranges and closes top cap 2 after covering another carousel 3 in the top after finishing again, with fixed slot 22 and top carousel 3 joint, starter motor 13, motor 13 drives carousel 3 through connecting axle 14 and rotates, the solution in the centrifugal tube 4 filters nucleic acid through nucleic acid filtration membrane 44 under the effect of centrifugal force and only divides liquid pipe 41 left end, close motor 13 after rotatory certain time, open top cap 2, take out 4, lead out nucleic acid filtration membrane 44 left and right sides liquid respectively, obtain nucleic acid solution.

In summary, the purification device for nucleic acid extraction has the advantages that the motor 13 is arranged to drive the rotating disc 3 to rotate, the centrifugal tube 4 is clamped in the rotating disc 3, the cell suspension mixed with the cell membrane disruption reagent is placed in the centrifugal tube 4, the centrifugal tube 4 rotates under the drive of the motor 13 to continuously throw the mixed solution in the centrifugal tube to the nucleic acid filtering membrane 44, and nucleic acid can pass through the nucleic acid filtering membrane 44, so that the nucleic acid is separated from the cell impurity mixed solution, the nucleic acid separation speed is increased, the nucleic acid separation steps are reduced, and the purpose of convenient operation is achieved; through setting up carousel 3 and driving centrifuging tube 4 and rotate, the continuous effort of giving solution in the pipe to nucleic acid filtration membrane 44 removal fully filters the nucleic acid in the solution, has reduced accounting residue in the waste material in the traditional purification process and the waste material content in the finished product to reach the mesh of improving the purity.

Claims

1. The utility model provides a purification device that nucleic acid extraction used, includes box (1), top cap (2), carousel (3) and centrifuging tube (4), its characterized in that: a rotating shaft (11) is fixedly installed behind the upper surface of the box body (1), a placement groove (12) is formed in the upper surface of the box body (1), a motor (13) is fixedly installed inside the box body (1), a rotor of the motor (13) is fixedly connected with a connecting shaft (14), and the box body (1) is rotatably connected with a top cover (2) through the rotating shaft (11);

a frame groove (21) is formed in the lower surface of the top cover (2), and a fixing groove (22) is formed in the middle of the frame groove (21);

a turntable (3) is fixedly mounted at the upper end of the connecting shaft (14), a counterweight ring (31) is fixedly mounted at the outer edge of the turntable (3), and a limiting groove (32) is formed in the upper surface of the counterweight ring (31);

the inside joint of spacing groove (32) has centrifuging tube (4), the right-hand member threaded connection of centrifuging tube (4) has pipe cap (43), the left end threaded connection of centrifuging tube (4) has branch liquid pipe (41), the inside fixed mounting of branch liquid pipe (41) has nucleic acid filtration membrane (44), the one end surface that divides liquid pipe (41) to be close to centrifuging tube (4) covers there is rubber slipmat (42).

2. The purification apparatus for nucleic acid isolation according to claim 1, wherein: the number of the arrangement grooves (12) is two, two the inside of the arrangement grooves (12) is connected with a rotary table (3) in a rotating mode, and the rotary table (3) limiting grooves (32) in the same arrangement grooves (12) are placed above the rotary tables (3) in the two arrangement grooves (12) in a relative mode.

3. The purification apparatus for nucleic acid isolation according to claim 1, wherein: the both ends of centrifuging tube (4) all are nested inside branch liquid pipe (41) and tube cap (43), centrifuging tube (4) and branch liquid pipe (41) internal diameter equal.

4. The purification apparatus for nucleic acid isolation according to claim 1, wherein: when the top cover (2) is closed on the upper surface of the box body (1), the fixing groove (22) is clamped at the outer edge of the turntable (3) above the corresponding placing groove (12).

5. The purification apparatus for nucleic acid isolation according to claim 1, wherein: the diameter of the tube cap (43) is larger than that of the centrifuge tube (4).

6. The purification apparatus for nucleic acid isolation according to claim 1, wherein: the cross-sectional diameter of one end of the limiting groove (32) close to the circle center of the placing groove (12) is larger than the cross-sectional diameter of one end of the placing groove (12) far away from the circle center of the placing groove (12), and the shape and the size of the limiting groove (32) are matched with those of the centrifugal tube (4) and the tube cap (43).