CN211838499U - Centrifugal device suitable for nucleic acid grinding and separating - Google Patents
Centrifugal device suitable for nucleic acid grinding and separating Download PDFInfo
- Publication number
- CN211838499U CN211838499U CN201922489762.3U CN201922489762U CN211838499U CN 211838499 U CN211838499 U CN 211838499U CN 201922489762 U CN201922489762 U CN 201922489762U CN 211838499 U CN211838499 U CN 211838499U
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- China
- Prior art keywords
- nucleic acid
- grinding
- pipe
- centrifugal device
- mount
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 30
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 30
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 30
- 238000000227 grinding Methods 0.000 title claims abstract description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 46
- 238000000926 separation method Methods 0.000 claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 23
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 23
- 230000005540 biological transmission Effects 0.000 claims abstract description 5
- 238000002955 isolation Methods 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 6
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 238000000498 ball milling Methods 0.000 description 2
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
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- Centrifugal Separators (AREA)
Abstract
The utility model provides a pair of centrifugal device suitable for nucleic acid grinding separation, which comprises a housin, casing one side is equipped with the motor, and casing one side is equipped with sun gear and mount in addition, is equipped with between sun gear and the mount and places the pipe, places pipe and sun gear, mount transmission and connects, places to be equipped with the liquid nitrogen storehouse between the pipe, is equipped with liquid nitrogen cang gai on the liquid nitrogen storehouse, is equipped with the fixed plate on the mount, fixed plate and place and be equipped with swing joint mechanism between the pipe. The utility model provides a pair of centrifugal device suitable for nucleic acid grinds separation utilizes planetary nucleic acid to grind the isolation structure, and test material is extravagant few, once can grind the separation to a plurality of samples simultaneously, and separation efficiency is high, and the separation effect is high, washs the convenience moreover, and the temperature controllability is good, has extensive suitability in the nucleic acid separation field.
Description
Technical Field
The utility model belongs to the field of test apparatus, it is specific, the utility model relates to a technical field that nucleic acid separation was equipped.
Background
Nucleic acid isolation and identification is a general term for a series of biological techniques for biochemically purifying nucleic acid substances in biological tissues and comparing the specificity of the compositions or structures of the nucleic acid substances. Is commonly used in genetics, developmental biology, molecular taxonomy, medicine and gene manipulation. The common means for DNA isolation include phenol method, isoamyl alcohol method, and concentrated salt method.
The phenol process for extracting DNA from animal tissue includes such steps as homogenizing tissue, extracting and breaking cell nucleus, extracting phenol to remove protein, depositing in alcohol, dialysis, treating with protease and DNase, and electrophoretic separation. Wherein crushing and separating usually uses grinding and centrifugal separation equipment.
The centrifugal machine used for centrifugal separation in the existing device grinds and crushes by ball milling methods such as zirconia balls and the like, but the separation of grinding and separation has serious material loss and low separation efficiency, and the test progress is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model discloses it is serious to distribute among the prior art to grind, separate experimental material loss, and separation efficiency is low, influences the technical problem of experimental progress, the utility model provides a centrifugal device suitable for nucleic acid grinding and separating utilizes the experimental limit of planet ball-milling structure to grind the limit and separates, and test efficiency is high, and the material is extravagant few, and experimental convenient operation has extensive suitability in nucleic acid separation field.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a pair of centrifugal device suitable for nucleic acid grinding separation, which comprises a housin, casing one side is equipped with the motor, and casing one side is equipped with sun gear and mount in addition, is equipped with between sun gear and the mount and places the pipe, places pipe and sun gear, mount transmission and connects, places to be equipped with the liquid nitrogen storehouse between the pipe, is equipped with liquid nitrogen cang gai on the liquid nitrogen storehouse, is equipped with the fixed plate on the mount, fixed plate and place and be equipped with swing joint mechanism between the pipe.
The utility model discloses in, the fixed plate with place and be equipped with the spring between the pipe, the spring center is equipped with the gag lever post, be equipped with the annular groove that suits with the gag lever post on the fixed plate.
The utility model discloses in, be equipped with fastening device between fixed plate and the liquid nitrogen storehouse lid.
The utility model discloses in, it is equipped with on the pipe to place the tube cap, places to be equipped with the gyro wheel between tube cap and the fixed plate.
The utility model discloses in, the sun gear lower part is equipped with the mounting panel.
The utility model discloses in, the mounting panel has the derailleur, and derailleur and motor pass through the belt and connect.
The utility model discloses in, be equipped with the fastening frame on the mount, be equipped with the fixed plate between the fastening frame.
The utility model discloses in, motor upper portion is equipped with the converter, converter and motor electric connection.
The utility model discloses in, one side on casing upper portion is equipped with flip.
The utility model discloses in, place the pipe and be equipped with the tapered gear.
The utility model discloses a following beneficial effect has:
the utility model provides a pair of centrifugal device suitable for nucleic acid grinds separation utilizes planetary nucleic acid to grind the isolation structure, and test material is extravagant few, once can grind the separation to a plurality of samples simultaneously, and separation efficiency is high, and the separation effect is high, washs the convenience moreover, and the temperature controllability is good, has extensive suitability in the nucleic acid separation field.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.
Fig. 1 shows the structure of the present invention.
Fig. 2 is a schematic sectional view of the present invention.
Fig. 3 is a schematic view of the gear connection structure of the present invention.
Fig. 4 is a schematic view of the placing tube structure of the present invention.
Fig. 5 is a schematic view of the liquid nitrogen storage chamber of the present invention.
In FIGS. 1-5: 1-shell, 2-motor, 3-belt, 4-speed changer, 5-central gear, 6-placing tube, 7-fixing frame, 8-mounting plate, 9-liquid nitrogen bin cover, 10-limiting rod, 11-spring, 12-liquid nitrogen bin, 13-fixing plate, 14-fastening frame, 15-fastening mechanism and 16-frequency converter.
Detailed Description
The following description of the present invention will be made in further detail with reference to the accompanying fig. 1 to 5 and examples, but the present invention is not limited to the following examples.
In the present invention, for the sake of convenience and description, the description of the relative positional relationship of the respective parts in a centrifugal apparatus suitable for nucleic acid grinding and separation is described based on the layout of FIG. 1, as follows: the positional relationship of up, down, left, right, etc. is determined according to the layout direction of fig. 1.
The utility model discloses in the casing 1 that uses, motor 2, derailleur 4, sun gear 5 places pipe 6, mount 7, mounting panel 8, liquid nitrogen cang gai 9, gag lever post 10, spring 11, fixed plate 13, fastening device 15, converter 16 etc. can both purchase or the customization obtains through ordinary market approach.
The utility model provides a pair of centrifugal device suitable for nucleic acid grinding separation, including casing 1, 1 one side of casing is equipped with motor 2, and 1 other one side of casing is equipped with sun gear 5 and mount 7, is equipped with between sun gear 5 and the mount 7 and places pipe 6, places pipe 6 and sun gear 5, the transmission of mount 7 is connected, places and is equipped with liquid nitrogen storehouse 12 between the pipe 6, is equipped with liquid nitrogen cang gai 9 on the liquid nitrogen storehouse 12, is equipped with fixed plate 13 on the mount 7, fixed plate 13 and place and be equipped with swing joint mechanism between the pipe 6, sun gear 5 and the transmission of motor 2 are connected.
The utility model discloses in, fixed plate 13 with place and be equipped with spring 11 between the pipe 6, spring 11 center is equipped with gag lever post 10, be equipped with the annular groove who suits with gag lever post 10 on the fixed plate 13.
The utility model discloses in, be equipped with fastening device 15 between fixed plate 13 and the liquid nitrogen storehouse lid 9.
The utility model discloses in, it places the pipe cap to be equipped with on the pipe 6 to place, places and is equipped with the gyro wheel between pipe cap and the fixed plate 13.
The utility model discloses in, 5 lower parts of sun gear are equipped with mounting panel 8.
The utility model discloses in, mounting panel 8 has derailleur 4, and derailleur 4 and motor 2 pass through belt 3 and connect.
The utility model discloses in, be equipped with fastening frame 14 on the mount 7, be equipped with fixed plate 13 between the fastening frame 14.
The utility model discloses in, 2 upper portions of motor are equipped with converter 16, 16 and the 2 electric connection of motor of converter.
In the utility model, one side of the upper part of the shell 1 is provided with a flip cover.
The utility model discloses in, place pipe 6 and be equipped with the tapered gear down.
Be suitable for during centrifugal device suitable for nucleic acid grinding separation, pour into the liquid nitrogen into in liquid nitrogen storehouse 12, cover liquid nitrogen storehouse lid 9, to check out experimental sample and put into and place pipe 6, place and put into the zirconia ball in the pipe 6, will place pipe 6 symmetry and insert between sun gear 5 and mount 7, cover and place the pipe lid, insert fixed plate 13 to the fastening frame 14 in, press mount 7 downwards with fastening device 15's screw, it places the pipe lid to push down, liquid nitrogen storehouse lid 9, guarantee to seal well, open 16 starter motor 2 of converter, sun gear 5 is rotatory, it is rotatory to drive to place pipe 6 and do the planet around sun gear 5, the ball that the pipe the inside was placed in the drive grinds nucleic acid, thereby adjust 16 control rotational speeds of converter and realize the centrifugation.
The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the above embodiments are only applicable to help understand the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the description should not be construed as a limitation to the present invention.
Claims (10)
1. The utility model provides a centrifugal device suitable for nucleic acid grinding separation, includes the casing, its characterized in that, casing one side is equipped with the motor, and other one side of casing is equipped with sun gear and mount, and sun gear and motor drive are connected, are equipped with between sun gear and the mount and place the pipe, place pipe and sun gear, mount transmission and connect, place and be equipped with the liquid nitrogen storehouse between the pipe, are equipped with liquid nitrogen storehouse lid on the liquid nitrogen storehouse, are equipped with the fixed plate on the mount, the fixed plate with place and be equipped with swing joint mechanism between the pipe.
2. The centrifugal device for grinding and separating nucleic acid as claimed in claim 1, wherein a spring is disposed between the fixing plate and the placing tube, a limiting rod is disposed at the center of the spring, and an annular groove corresponding to the limiting rod is disposed on the fixing plate.
3. The centrifugal device for grinding and separating nucleic acid as claimed in claim 1, wherein a fastening mechanism is provided between the fixing plate and the liquid nitrogen cap.
4. The centrifuge apparatus for nucleic acid isolation by grinding according to claim 1, wherein the placing tube has a placing tube cover, and a roller is disposed between the placing tube cover and the fixing plate.
5. The centrifugal device for grinding and separating nucleic acid as claimed in claim 1, wherein a mounting plate is provided at a lower portion of the sun gear.
6. The centrifugal device for grinding and separating nucleic acid as claimed in claim 5, wherein a speed changer is arranged below the mounting plate, and the speed changer and the motor are connected through a belt.
7. The centrifugal device for grinding and separating nucleic acid as claimed in claim 2, wherein the fixing frame is provided with fastening frames, and a fixing plate is arranged between the fastening frames.
8. The centrifugal apparatus for nucleic acid grinding and separating according to claim 1, wherein the motor is provided with a frequency converter at the upper part, and the frequency converter is electrically connected with the motor.
9. The centrifugal device for nucleic acid grinding and separating of claim 1, wherein one side of the upper part of the housing is provided with a flip.
10. The centrifugal device for grinding and separating nucleic acid as claimed in claim 1, wherein a bevel gear is arranged below the placing tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922489762.3U CN211838499U (en) | 2019-12-31 | 2019-12-31 | Centrifugal device suitable for nucleic acid grinding and separating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922489762.3U CN211838499U (en) | 2019-12-31 | 2019-12-31 | Centrifugal device suitable for nucleic acid grinding and separating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211838499U true CN211838499U (en) | 2020-11-03 |
Family
ID=73209875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922489762.3U Expired - Fee Related CN211838499U (en) | 2019-12-31 | 2019-12-31 | Centrifugal device suitable for nucleic acid grinding and separating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211838499U (en) |
-
2019
- 2019-12-31 CN CN201922489762.3U patent/CN211838499U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201103 |
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| CF01 | Termination of patent right due to non-payment of annual fee |