CN213708373U - A separation and purification device for RNA - Google Patents
A separation and purification device for RNA Download PDFInfo
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- CN213708373U CN213708373U CN202022603257.XU CN202022603257U CN213708373U CN 213708373 U CN213708373 U CN 213708373U CN 202022603257 U CN202022603257 U CN 202022603257U CN 213708373 U CN213708373 U CN 213708373U
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Abstract
The utility model discloses a separation and purification device for RNA, which relates to the technical field of biological pharmacy devices. The utility model discloses a body and footstock: a motor is arranged in the top seat; the body is internally distributed with a fixing ring; the fixing ring is internally provided with a shaft sleeve; a rotating cup is distributed in the shaft sleeve; a connector is arranged above the rotating cup; the connecting body is provided with a cover body; the cover body is connected with the output end of the motor; a vibration cup is also distributed in the rotary cup; the inner wall of the rotating cup is provided with a vibration motor which is electrically connected with the PLC; a fixed seat is also arranged in the body; a collecting tray is also arranged in the fixed seat; a collecting cylinder is arranged below the collecting disc corresponding to the body; one side of the collecting cylinder is communicated with an eluent inlet, and the bottom of the collecting cylinder is communicated with a second liquid outlet pipe. The utility model provides a separation and purification device for RNA, its misce bene separates thoroughly, and is efficient, can mix the liquid to carry out the quick separation purification to RNA, and is convenient for collect the product after the purification.
Description
Technical Field
The utility model belongs to the technical field of bio-pharmaceuticals device, concretely relates to a separation and purification device for RNA.
Background
There are more than one microbial culture known as mixed cultures. If all cells in a colony are from a parent cell, the colony is said to be a pure culture. In the case of species identification, the microorganisms used are generally required to be pure cultures. The process of obtaining pure cultures is called isolation and purification.
Most of separation and purification devices at the present stage are operated independently, and the work flow is more; the existing separation and purification device cannot completely separate the mixed liquid, so that the purified product still contains impurities, and the separation and purification speed is low and the efficiency is low.
To above circumstances, the present demand a separation and purification device for RNA has the misce bene, separates thoroughly, efficient advantage, can carry out the rapid separation purification to the mixed liquid of RNA, and is convenient for collect the product after the purification.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a separation and purification device for RNA realizes the misce bene, and the separation is thorough, and efficient purpose can carry out the quick separation purification to the mixed liquid of RNA, and is convenient for collect the product after the purification.
The utility model provides a following technical scheme:
an apparatus for separating and purifying RNA, comprising a body and a top seat: the top seat is arranged above the body; a motor is arranged in the top seat; the outer wall of the body is provided with a PLC electrically connected with the motor; supporting legs are arranged below the body.
The body is internally provided with a fixing ring; the fixing ring is internally provided with a shaft sleeve; a rotating cup is distributed in the shaft sleeve; a connecting body is arranged above the rotating cup; the connecting body is provided with a cover body; the cover body is connected with the output end of the motor; a vibration cup is also distributed in the rotary cup; the inner wall of the rotating cup is provided with a vibration motor which is electrically connected with the PLC; the output end of the vibration motor is connected with the vibration cup; a filter screen is also distributed in the vibration cup; the two sides of the vibrating cup are also communicated with a first outlet; the bottom of the vibrating cup is communicated with an inner liquid outlet pipe; the bottom of the rotating cup is communicated with an outer liquid outlet pipe sleeved on the periphery of the inner liquid outlet pipe; a liquid inlet pipe is also arranged in the top seat; the top of the liquid inlet pipe extends out of the top seat, and the bottom of the liquid inlet pipe extends into the vibrating cup.
A fixed seat is also arranged in the body; a collecting tray is also arranged in the fixed seat; a first liquid outlet pipe is communicated with the lower part of the collecting tray; a collecting cylinder is arranged below the collecting disc corresponding to the body; one side of the collecting cylinder is communicated with an eluent inlet, and the bottom of the collecting cylinder is communicated with a second liquid outlet pipe.
Preferably, a round hole is formed in the connecting body; the liquid inlet pipe penetrates through the round hole and extends into the vibration cup.
Preferably, the first outlet is arranged above the filter screen.
Preferably, the inner liquid outlet pipe penetrates through the collecting tray and is arranged above the collecting cylinder.
Preferably, a peristaltic pump electrically connected with the PLC is further arranged on the first liquid outlet pipe.
Preferably, a gap is reserved between the rotating cup and the vibrating cup; the outer port of the first outlet is arranged in the rotating cup.
Preferably, the inner liquid outlet pipe is further provided with an electromagnetic valve electrically connected with the PLC.
The utility model discloses a profitable effect:
the utility model is provided with the vibration motor which can fully mix the mixed liquid added into the body; the added filter screen can filter and remove impurities from the mixed liquid; the additional motor can drive the rotating cup and the vibrating cup in the rotating cup to rotate for high-speed centrifugation, and RNA precipitate in the mixed solution is separated; the added collecting cylinder can collect RNA sediment, eluent is added into the collecting cylinder, and the RNA sediment is eluted to obtain pure RNA. The utility model discloses reached the misce bene, the separation is thorough, and efficient effect can carry out the quickly separating purification to the mixed liquid of RNA, and is convenient for collect the product after the purification.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a plan view of the present invention.
Description of reference numerals:
1. a body; 101. a PLC controller; 102. supporting legs; 2. a top seat; 201. a liquid inlet pipe; 202. a motor; 3. a cover body; 301. a linker; 4. rotating the cup; 401. an outlet liquid pipe; 5. vibrating the cup; 501. a first outlet; 502. an inner liquid outlet pipe; 6. a fixing ring; 601. a shaft sleeve; 7. a fixed seat; 8. a collection tray; 801. a first liquid outlet pipe; 802. a peristaltic pump; 9. a collection canister; 901. a second liquid outlet pipe; 902. an eluent inlet; 10. filtering with a screen; 11. a vibration motor.
Detailed Description
As shown in the figure, the device for separating and purifying RNA comprises a body 1 and a top seat 2: the top seat 2 is arranged above the body 1; a liquid inlet pipe 201 is also arranged in the top seat 2; a circular hole is formed inside the connecting body 301; the liquid inlet pipe 201 penetrates through the round hole and extends into the vibration cup 5 in the body 1; the top of the liquid inlet pipe 201 extends out of the top seat 2; a motor 202 is arranged in the top seat 2; the outer wall of the body 1 is provided with a PLC (programmable logic controller) 101 which is electrically connected with a motor 202; supporting legs 102 are arranged below the body 1.
A fixing ring 6 is distributed in the body 1; a shaft sleeve 601 is arranged in the fixing ring 6; a rotating cup 4 is arranged in the shaft sleeve 601; a connecting body 301 is arranged above the rotating cup 4; the connecting body 301 is provided with a cover body 3; the cover body 3 is connected with the output end of the motor 202; a vibration cup 5 is also distributed in the rotary cup 4; a gap is reserved between the rotary cup 4 and the vibration cup 5; a vibration motor 11 electrically connected with the PLC 101 is distributed on the inner wall of the rotating cup 4; the output end of the vibration motor 11 is connected with the vibration cup 5; a filter screen 10 is also arranged in the vibration cup 5.
The vibration motor 11 is started through the PLC 101, the vibration cup 4 is driven to vibrate by the work of the vibration motor 11, so that the mixed liquid in the vibration cup 4 is fully mixed, the contact area of the mixed liquid and the filter screen 10 is increased, and the filtering is fully carried out; then, the motor 202 is started, the motor 202 works to drive the cover body 3, the connecting body 301 and the rotating cup 5 to rotate, and the vibrating cup 4 arranged in the rotating cup 5 rotates along with the rotating cup 5; the mixed liquid in the vibrating cup 4 is subjected to high-speed centrifugal motion, so that the mixed liquid achieves the layering effect; and the lateral wall of the body 1 is provided with a transparent observation window, and the layering condition of the mixed liquid can be observed through the transparent observation window.
Two sides of the vibrating cup 5 are also communicated with a first outlet 501; the first outlet 501 is arranged above the filter screen 10; the outer port of the first outlet 501 is arranged in the rotating cup 4; the bottom of the vibrating cup 5 is communicated with an inner liquid outlet pipe 502; the inner liquid outlet pipe 502 is also provided with an electromagnetic valve electrically connected with the PLC 101; the bottom of the rotating cup 4 is communicated with an outer liquid outlet pipe 401 which is sleeved on the periphery of the inner liquid outlet pipe 502; a fixed seat 7 is also arranged in the body 1; a collecting tray 8 is also arranged in the fixed seat 7; a first liquid outlet pipe 801 is communicated with the lower part of the collecting tray 8; a peristaltic pump 802 electrically connected with the PLC 101 is also arranged on the first liquid outlet pipe 801; under the action of the peristaltic pump 802, all the liquid in the collecting tray 8 flows out of the body 1 from the first liquid outlet pipe 801; a collecting cylinder 9 is arranged below the body 1 corresponding to the collecting tray 8; the inner outlet pipe 502 is arranged above the collecting cylinder 9 through the collecting tray 8.
Under the filtering action of the filter screen 1, the components which cannot pass through the filter screen 10 of the mixed liquid in the vibrating cup 5 flow into the rotating cup 4 from the first outlet 501, flow out of the rotating cup 4 along the liquid outlet pipe 401 at the bottom of the rotating cup 4, fall into the collecting tray 8, and the liquid in the collecting tray 8 flows out of the body 1 from the first liquid outlet pipe 801; while the components passing through the sieve 10 will flow out of the vibrating cup 5 through the inner outlet pipe 502; falls into a collecting cylinder 9 arranged below the collecting tray 8.
One side of the collecting cylinder 9 is communicated with an eluent inlet 902, and the bottom of the collecting cylinder is communicated with a second liquid outlet pipe 901; eluent is added into the collection cylinder 9 from the eluent inlet 902 to elute the mixed solution, and the eluted RNA product is collected from the second liquid outlet 901 at the bottom of the collection cylinder 9.
The utility model discloses a working method: adding the mixed liquid to be separated and purified into the body 1 from the feeding pipe 201, and placing different collecting pipes below the first liquid outlet pipe 801 and the second liquid outlet pipe 901 respectively; the electromagnetic valve is closed through the PLC 101, the vibration motor 11 is started, the working time of the vibration motor 11 is set, and the vibration cup 5 is driven to vibrate through the vibration motor 11, so that the mixed liquid is vibrated and filtered; after the vibration motor 11 finishes working after reaching the set time, the motor 202 is started, the motor 202 works to enable the mixed liquid in the vibration cup 5 to be subjected to high-speed centrifugation to obtain RNA precipitate, the electromagnetic valve is opened to enable the RNA precipitate to flow into the collection cylinder 9, then eluent is added into the collection cylinder 9, and after the elution is finished, the separated and purified pure RNA stock solution can be obtained from the second liquid outlet pipe 901.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A separation and purification device for RNA, which comprises a body (1) and a top seat (2), and is characterized in that: the top seat (2) is arranged above the body (1); a motor (202) is arranged in the top seat (2); the outer wall of the body (1) is provided with a PLC (101) electrically connected with the motor (202); supporting legs (102) are arranged below the body (1);
a fixing ring (6) is distributed in the body (1); a shaft sleeve (601) is distributed in the fixing ring (6); a rotating cup (4) is distributed in the shaft sleeve (601); a connecting body (301) is arranged above the rotating cup (4); a cover body (3) is arranged on the connecting body (301); the cover body (3) is connected with the output end of the motor (202); a vibration cup (5) is also distributed in the rotary cup (4); a vibration motor (11) electrically connected with the PLC (101) is distributed on the inner wall of the rotary cup (4); the output end of the vibration motor (11) is connected with the vibration cup (5); a filter screen (10) is also distributed in the vibration cup (5); two sides of the vibrating cup (5) are also communicated with a first outlet (501); the bottom of the vibrating cup (5) is communicated with an inner liquid outlet pipe (502); the bottom of the rotary cup (4) is communicated with an outer liquid outlet pipe (401) which is sleeved on the periphery of the inner liquid outlet pipe (502); a liquid inlet pipe (201) is also arranged in the top seat (2); the top of the liquid inlet pipe (201) extends out of the top seat (2), and the bottom of the liquid inlet pipe extends into the vibrating cup (5);
a fixed seat (7) is also arranged in the body (1); a collecting tray (8) is also arranged in the fixed seat (7); a first liquid outlet pipe (801) is communicated with the lower part of the collecting tray (8); a collecting cylinder (9) is also arranged below the body (1) corresponding to the collecting tray (8); one side of the collecting cylinder (9) is communicated with an eluent inlet (902), and the bottom of the collecting cylinder is communicated with a second liquid outlet pipe (901).
2. The device for separating and purifying RNA according to claim 1, wherein: a round hole is formed in the connecting body (301); the liquid inlet pipe (201) penetrates through the round hole and extends into the vibration cup (5).
3. The device for separating and purifying RNA according to claim 1, wherein: the first outlet (501) is arranged above the filter screen (10).
4. The device for separating and purifying RNA according to claim 1, wherein: the inner liquid outlet pipe (502) penetrates through the collecting tray (8) and is arranged above the collecting cylinder (9).
5. The device for separating and purifying RNA according to claim 1, wherein: the first liquid outlet pipe (801) is further provided with a peristaltic pump (802) electrically connected with the PLC (101).
6. The device for separating and purifying RNA according to claim 1, wherein: a gap is reserved between the rotary cup (4) and the vibration cup (5); the outer port of the first outlet (501) is distributed in the rotating cup (4).
7. The device for separating and purifying RNA according to claim 1, wherein: and the inner liquid outlet pipe (502) is also provided with an electromagnetic valve electrically connected with the PLC (101).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022603257.XU CN213708373U (en) | 2020-11-11 | 2020-11-11 | A separation and purification device for RNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022603257.XU CN213708373U (en) | 2020-11-11 | 2020-11-11 | A separation and purification device for RNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213708373U true CN213708373U (en) | 2021-07-16 |
Family
ID=76804747
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022603257.XU Active CN213708373U (en) | 2020-11-11 | 2020-11-11 | A separation and purification device for RNA |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213708373U (en) |
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2020
- 2020-11-11 CN CN202022603257.XU patent/CN213708373U/en active Active
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