CN211620485U

CN211620485U - Special ultrafiltration of exosome extraction and separation, super integrative device that leaves

Info

Publication number: CN211620485U
Application number: CN201921990885.9U
Authority: CN
Inventors: 陈锦阳; 乔博超; 刘军权
Original assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Current assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Priority date: 2019-11-18
Filing date: 2019-11-18
Publication date: 2020-10-02
Anticipated expiration: 2029-11-18

Abstract

The utility model relates to an exosome extraction and separation technical field specifically is a special ultrafiltration of exosome extraction and separation, super integrative device, including the support, be equipped with the centrifuge bowl on the support, centrifuge bowl bottom is connected with first drain pipe, and first drain pipe bottom is connected with first shunt and second shunt, is equipped with the cartridge filter on the support, and inside the terminal of second shunt extended to the cartridge filter, it is equipped with the milipore filter to lie in the terminal below of second shunt on the cartridge filter inner wall, is equipped with the third drain pipe on the cartridge filter lateral wall, and the bottom is equipped with the fourth drain pipe. This special ultrafiltration of exosome extraction separation, super from integrative device, liquid in the centrifuge bowl after the centrifugation layering, the back directly gets into the cartridge filter and filters, improves extraction efficiency, has reduced exosome extract and external contact simultaneously, and then reduces the contaminated possibility of exosome extract.

Description

Special ultrafiltration of exosome extraction and separation, super integrative device that leaves

Technical Field

The utility model relates to an exosome extraction and separation technical field specifically is a special ultrafiltration of exosome extraction and separation, surpasses from integrative device.

Background

Exosome is a tiny vesicle secreted by cells, naturally exists in biological body fluids such as blood, urine, saliva, breast milk, cell culture medium and the like, the extraction and separation of exosome have important value in biomedical research, the extraction of exosome is usually obtained by centrifugal filtration, the existing centrifugation and filtration are generally carried out separately, the extraction efficiency is low, and the exosome is easily polluted.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a special ultrafiltration of exosome extraction separation, surpass from integrative device to solve the problem of proposing in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

an ultrafiltration and super-separation integrated device special for exosome extraction and separation comprises a support, wherein a centrifugal cylinder is arranged on the support and is rotatably connected with the support, a first connecting pipe communicated with the centrifugal cylinder is arranged at the middle position of the top of the centrifugal cylinder, a second connecting pipe communicated with the centrifugal cylinder is arranged at the middle position of the bottom of the centrifugal cylinder, a driving wheel is sleeved on the outer side wall of the first connecting pipe, a high-speed motor is arranged at the side end of the centrifugal cylinder at the top of the support, an output shaft of the high-speed motor is vertically upward, the tail end of the output shaft of the high-speed motor is coaxially connected with a driving wheel, the driving wheel is connected with the driving wheel through a belt, a support plate is arranged below the centrifugal cylinder on the support, a through hole is formed under the second connecting pipe on the support plate, a mechanical sealing element is arranged in the through, mechanical seal's quiet ring bottom is connected with first drain pipe, first drain pipe bottom is equipped with respectively rather than the first shunt and the second shunt that are linked together, lie in on the support the high-speed motor side with be equipped with the cartridge filter, the end of second shunt extends to inside the cartridge filter, lie in on the cartridge filter inner wall the terminal below of second shunt is equipped with the milipore filter, be close to on the cartridge filter lateral wall milipore filter top edge is equipped with the third drain pipe, just the third drain pipe with the inside of cartridge filter is linked together, cartridge filter bottom intermediate position is equipped with the fourth drain pipe that is linked together with its inside.

Preferably, the outer side wall of the centrifugal cylinder is provided with two annular connecting blocks which are parallel to each other, and the bottoms and the tops of the two annular connecting blocks are respectively attached to the top and the bottom of the support.

Preferably, a feeding hopper communicated with the first connecting pipe is arranged at the top of the first connecting pipe.

Preferably, the first shunt pipe and the second shunt pipe are respectively provided with a first control valve and a second control valve.

Preferably, an annular fixing block is arranged below the tail end of the second shunt pipe on the inner wall of the filter cylinder, and the ultrafiltration membrane is fixed on the annular fixing block.

Preferably, a third control valve and a fourth control valve are respectively arranged on the third liquid outlet pipe and the fourth liquid outlet pipe.

Preferably, the pneumatic cylinder is installed at the cartridge filter top, the vertical downwardly extending of piston rod of pneumatic cylinder to inside the cartridge filter, just the piston rod end of pneumatic cylinder is equipped with the piston, the external diameter size of piston with the internal diameter size looks adaptation of cartridge filter and rather than sliding connection.

Compared with the prior art, the beneficial effects of the utility model are that:

this special ultrafiltration of exosome extraction separation, super from integrative device, through the first drain pipe that sets up, liquid is after the centrifugation layering in the centrifuge bowl, lower floor's impurity flows from first shunt, upper strata exosome extract filters in the second shunt inflow cartridge filter again, further purifies exosome extract, directly filter after the centrifugation simultaneously, improve extraction efficiency, reduced exosome extract and external contact simultaneously, and then reduce the contaminated possibility of exosome extract.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an enlarged view of the position A in the overall structural diagram of the present invention;

FIG. 3 is a schematic structural view of a centrifugal cylinder of the present invention;

fig. 4 is a schematic structural view of the support plate of the present invention;

FIG. 5 is a schematic structural view of a first liquid outlet pipe of the present invention;

fig. 6 is a schematic structural view of a filter cartridge according to the present invention;

fig. 7 is a cross-sectional view of a filter cartridge according to the present invention.

In the figure: 1. a support; 2. a centrifugal cylinder; 20. a first connecting pipe; 21. a feed hopper; 22. a second connecting pipe; 23. an annular connecting block; 3. a support plate; 30. a mechanical seal; 4. a high-speed motor; 5. a driving wheel; 6. a drive wheel; 7. a belt; 8. a first liquid outlet pipe; 80. a first shunt pipe; 800. a first control valve; 81. a second shunt pipe; 810. a second control valve; 9. a filter cartridge; 90. an annular fixed block; 91. ultrafiltration membranes; 92. a third liquid outlet pipe; 920. a third control valve; 93. a fourth liquid outlet pipe; 930. a fourth control valve; 94. a hydraulic cylinder; 95. a piston.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second", "third", "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", "third", "fourth" may explicitly or implicitly include one or more of the features. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1-7, the present invention provides a technical solution:

an ultrafiltration and super-separation integrated device special for exosome extraction and separation comprises a support 1, a centrifugal cylinder 2 rotationally connected with the support 1 is arranged on the support 1, two annular connecting blocks 23 which are parallel to each other are arranged on the outer side wall of the centrifugal cylinder 2, the bottom and the top of the two annular connecting blocks 23 are respectively attached to the top and the bottom of the support 1, a first connecting pipe 20 communicated with the interior of the centrifugal cylinder 2 is arranged at the middle position of the top of the centrifugal cylinder 2, a feeding funnel 21 communicated with the first connecting pipe 20 is arranged at the top of the first connecting pipe 20, a second connecting pipe 22 communicated with the interior of the centrifugal cylinder 2 is arranged at the middle position of the bottom of the centrifugal cylinder 2, a driving wheel 6 is sleeved on the outer side wall of the first connecting pipe 20, a high-speed motor 4 is arranged at the side end of the top of the support 1, an output shaft of the high, the liquid to be extracted flows into the centrifugal cylinder 2 through the feeding hopper 21, the driving wheel 5 is driven to rotate by the rotation of the output shaft of the high-speed motor 4, and then the driving wheel 6 and the centrifugal cylinder 2 are driven to rotate by the belt 7, so that the liquid in the centrifugal cylinder 2 is driven to be centrifuged and layered.

In the embodiment, a support plate 3 is arranged below a centrifugal cylinder 2 on a support 1, a through hole is arranged on the support plate 3 and is positioned right below a second connecting pipe 22, a mechanical sealing element 30 is arranged in the through hole, the second connecting pipe 22 is connected with a moving ring of the mechanical sealing element 30, a first liquid outlet pipe 8 is connected with the bottom of a stationary ring of the mechanical sealing element 30, a first shunt pipe 80 and a second shunt pipe 81 which are communicated with the first liquid outlet pipe 8 are respectively arranged at the bottom of the first liquid outlet pipe 8, a first control valve 800 and a second control valve 810 are respectively arranged on the first shunt pipe 80 and the second shunt pipe 81, a filter cylinder 9 is arranged at the side end of a high-speed motor 4 on the support 1, the tail end of the second shunt pipe 81 extends into the filter cylinder 9, after centrifugal stratification in the centrifugal cylinder 2, the first control valve 800 is opened, after lower-layer impurities flow out from the first shunt pipe 80, the first control, the upper exosome extract flows into the filter cartridge 9 via the second shunt 81.

Besides, an annular fixing block 90 is arranged below the tail end of the second shunt pipe 81 on the inner wall of the filter cylinder 9, an ultrafiltration membrane 91 is fixed on the annular fixing block 90, a third liquid outlet pipe 92 is arranged on the outer side wall of the filter cylinder 9 and close to the top edge of the ultrafiltration membrane 91, the third liquid outlet pipe 92 is communicated with the inside of the filter cylinder 9, a fourth liquid outlet pipe 93 communicated with the inside of the filter cylinder is arranged in the middle of the bottom of the filter cylinder 9, a third control valve 920 and a fourth control valve 930 are respectively arranged on the third liquid outlet pipe 92 and the fourth liquid outlet pipe 93, an exosome extracting solution flows into the filter cylinder 9 through the second shunt pipe 81, water molecules in the exosome extracting solution are filtered by the ultrafiltration membrane 91, the water molecules flow into the bottom of the filter cylinder 9 for temporary storage, the fourth control valve 930 is periodically opened, the exosome extracting solution is discharged through the.

Further, the pneumatic cylinder 94 is installed at the filter cartridge 9 top, and the piston rod of pneumatic cylinder 94 is vertical downwardly extending to inside the filter cartridge 9, and the piston rod end of pneumatic cylinder 94 is equipped with piston 95, and the external diameter size of piston 95 and the internal diameter size looks adaptation of filter cartridge 9 and rather than sliding connection, the piston rod through pneumatic cylinder 94 drives piston 95 downstream, and the inside atmospheric pressure of increase filter cartridge 9 filters with higher speed.

It should be noted that the high-speed motor 4 in this embodiment may be a GDZ-80-1.5D motor manufactured by spacious motors ltd, japan, and its power source may be provided by the manufacturer; the ultrafiltration membrane 91 in the embodiment can be a flat plate type ultrafiltration membrane produced by Shenzhen Qijia spring membrane filtration equipment Limited company; the hydraulic cylinder 94 in this embodiment may be a hydraulic cylinder manufactured by pneumatic hydraulic limited, yangdaika, with a product model of MOB40 × 200-TC, and a hydraulic system and a pipeline matched with the hydraulic cylinder may be provided by the manufacturer; furthermore, the present invention relates to circuits, electronic components and modules, which are known in the art, and can be implemented by those skilled in the art without exception, and the present invention also does not relate to improvements to software and methods.

When the special ultrafiltration and superseparation integrated device for exosome extraction and separation is used, a worker firstly checks the integrity of the device; then the first control valve 800, the second control valve 810, the third control valve 920 and the fourth control valve 930 are closed, and the liquid to be extracted flows into the centrifuge tube 2 through the feeding funnel 21; then, a power supply of the high-speed motor 4 is switched on to enable the high-speed motor to work, an output shaft of the high-speed motor 4 rotates to drive a driving wheel 5 to rotate, and then a belt 7 drives a driving wheel 6 and the centrifugal cylinder 2 to rotate to drive liquid in the centrifugal cylinder 2 to carry out centrifugal layering; after the centrifugation is finished, the power supply of the high-speed motor 4 is cut off, and after the output shaft of the high-speed motor 4 stops rotating, the first control valve 800 is opened to flow out and collect the lower-layer impurities from the first shunt pipe 80; then the first control valve 800 is closed, the second control valve 810 is opened, and the upper layer exosome extract flows into the filter cylinder 9 through the second shunt pipe 81; then, hydraulic oil is injected into the hydraulic cylinder 94, a piston rod of the hydraulic cylinder 94 drives the piston 95 to move downwards, the air pressure inside the filter cartridge 9 is increased, and the filtering of water molecules in the exosome extract from the ultrafiltration membrane 91 is accelerated and the water molecules flow into the bottom of the filter cartridge 9 for temporary storage; after the filtering is completed, hydraulic oil is reversely injected into the hydraulic cylinder 94, the piston rod of the hydraulic cylinder 94 drives the piston 95 to return to the original position, the third control valve 920 is opened, and the exosome extract is discharged and collected from the third liquid outlet pipe 92.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a special ultrafiltration of exosome extraction separation, super from integrative device, includes support (1), its characterized in that: the centrifugal cylinder (2) is arranged on the support (1) and is rotationally connected with the support, a first connecting pipe (20) communicated with the interior of the centrifugal cylinder is arranged at the middle position of the top of the centrifugal cylinder (2), a second connecting pipe (22) communicated with the interior of the centrifugal cylinder is arranged at the middle position of the bottom of the centrifugal cylinder (2), a driving wheel (6) is sleeved on the outer side wall of the first connecting pipe (20), the top of the support (1) is positioned at the side end of the centrifugal cylinder (2) and is provided with a high-speed motor (4), an output shaft of the high-speed motor (4) is vertically upward, the tail end of the output shaft is coaxially connected with a driving wheel (5), the driving wheel (5) and the driving wheel (6) are connected through a belt (7), a support plate (3) is arranged below the centrifugal cylinder (2) on the support (1), a through hole is formed in, and a mechanical sealing element (30) is arranged in the through hole, the second connecting pipe (22) is connected with a moving ring of the mechanical sealing element (30), a first liquid outlet pipe (8) is connected to the bottom of a static ring of the mechanical sealing element (30), a first shunt pipe (80) and a second shunt pipe (81) communicated with the first liquid outlet pipe (8) are respectively arranged at the bottom of the first liquid outlet pipe (8), the bracket (1) is positioned at the side end of the high-speed motor (4) and provided with a filter cylinder (9), the tail end of the second shunt pipe (81) extends into the filter cylinder (9), an ultrafiltration membrane (91) is arranged below the tail end of the second shunt pipe (81) on the inner wall of the filter cylinder (9), a third liquid outlet pipe (92) is arranged on the outer side wall of the filter cylinder (9) near the top edge of the ultrafiltration membrane (91), and the third liquid outlet pipe (92) is communicated with, and a fourth liquid outlet pipe (93) communicated with the interior of the filter cartridge (9) is arranged in the middle of the bottom of the filter cartridge.

2. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: the centrifugal cylinder (2) is characterized in that two annular connecting blocks (23) which are parallel to each other are arranged on the outer side wall of the centrifugal cylinder (2), and the bottom and the top of each annular connecting block (23) are respectively attached to the top and the bottom of the support (1).

3. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: the top of the first connecting pipe (20) is provided with a feeding hopper (21) communicated with the first connecting pipe.

4. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: and a first control valve (800) and a second control valve (810) are respectively arranged on the first shunt pipe (80) and the second shunt pipe (81).

5. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: the filter cartridge (9) is characterized in that the inner wall of the filter cartridge is positioned below the tail end of the second shunt pipe (81), an annular fixing block (90) is arranged below the inner wall of the filter cartridge, and the ultrafiltration membrane (91) is fixed on the annular fixing block (90).

6. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: and a third control valve (920) and a fourth control valve (930) are respectively arranged on the third liquid outlet pipe (92) and the fourth liquid outlet pipe (93).

7. The special ultrafiltration and superseparation integrated device for exosome extraction and separation according to claim 1, which is characterized in that: pneumatic cylinder (94) are installed at filter cartridge (9) top, the vertical downwardly extending of piston rod of pneumatic cylinder (94) is to inside filter cartridge (9), just the piston rod end of pneumatic cylinder (94) is equipped with piston (95), the external diameter size of piston (95) with the internal diameter size looks adaptation of filter cartridge (9) and rather than sliding connection.