CN220125701U - Special ultrafiltration device for extracting and separating exosomes - Google Patents
Special ultrafiltration device for extracting and separating exosomes Download PDFInfo
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- CN220125701U CN220125701U CN202321631290.0U CN202321631290U CN220125701U CN 220125701 U CN220125701 U CN 220125701U CN 202321631290 U CN202321631290 U CN 202321631290U CN 220125701 U CN220125701 U CN 220125701U
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 42
- 238000000108 ultra-filtration Methods 0.000 title claims abstract description 23
- 238000000605 extraction Methods 0.000 claims abstract description 20
- 239000011324 bead Substances 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 238000000926 separation method Methods 0.000 claims abstract description 13
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 24
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 24
- 241001330002 Bambuseae Species 0.000 claims description 24
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 24
- 239000011425 bamboo Substances 0.000 claims description 24
- 229920000742 Cotton Polymers 0.000 claims description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 abstract description 3
- 239000012535 impurity Substances 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The utility model relates to the technical field of exosome extraction, and provides a special ultrafiltration device for exosome extraction and separation, which comprises a fixed table, a centrifugal cylinder and a motor, wherein the centrifugal cylinder is arranged on one side of the top end of the fixed table, a clamping structure is arranged on the outer side of the centrifugal cylinder, the motor is arranged on the other side of the top end of the fixed table, the bottom end of the motor is connected with the outer side of the centrifugal cylinder through a belt, a connecting pipe is arranged at the bottom end of the centrifugal cylinder, a filtering structure is arranged inside the connecting pipe, a collecting box is arranged on one side of the bottom end of the connecting pipe, an extraction cylinder is arranged on the other side of the bottom end of the connecting pipe, an air cylinder is arranged at the top end of the extraction cylinder, and an ultrafiltration membrane is arranged inside the extraction cylinder. According to the utility model, the clamping structure is arranged, so that the centrifugal barrel is more stable in rotation, and the centrifugal barrel can be clamped by the supporting rod, the clamping frame, the movable groove and the movable beads, so that the clamping effect is improved.
Description
Technical Field
The utility model relates to the technical field of exosome extraction, in particular to a special ultrafiltration device for exosome extraction and separation.
Background
Exosomes refer to small membrane vesicles (30-150 nm) containing complex RNAs and proteins, and nowadays, discoid vesicles with diameters of 40-100nm are specified, all cultured cell types can secrete exosomes, and exosomes naturally exist in body fluids, including blood, saliva, urine, cerebrospinal fluid and milk, and have great value in medical research, when exosomes are extracted, an extraction is usually carried out in a centrifugal way, and when extraction is carried out, a special ultrafiltration device for extracting and separating exosomes is used;
for this reason, the patent of publication number CN211620485U discloses a special ultrafiltration of exosome extraction separation, surpasses from integrative device, including the support, be equipped with centrifugal tube on the support, centrifugal tube bottom is connected with first drain pipe, first drain pipe bottom is connected with first shunt tubes and second shunt tubes, is equipped with the cartridge filter on the support, and the end of second shunt tubes extends to inside the cartridge filter, is located the terminal below of second shunt tubes on the cartridge filter inner wall and is equipped with the milipore filter, is equipped with the third drain pipe on the cartridge filter lateral wall, and the bottom is equipped with the fourth drain pipe. According to the special ultrafiltration and hyperseparation integrated device for extracting and separating the exosomes, liquid in the centrifugal barrel is subjected to centrifugal layering and then directly enters the filter cartridge for filtering, so that the extraction efficiency is improved, meanwhile, the contact between the exosome extracting solution and the outside is reduced, and the possibility of pollution of the exosome extracting solution is further reduced;
the above-mentioned prior art scheme has, and it is inconvenient to carry out spacing defect to it when driving the centrifugation section of thick bamboo to rotate, and the centrifugation section of thick bamboo needs high-speed to carry out a rotation when extracting the exosome, and this just leads to the centrifugation section of thick bamboo to appear the condition of position deviation easily at the in-process of high-speed rotation, consequently just needs to use an exosome to draw the special ultrafiltration device of separation to solve above-mentioned problem.
Disclosure of Invention
The utility model aims to provide an ultrafiltration device special for extracting and separating exosomes, which is used for solving the defect that the conventional ultrafiltration device special for extracting and separating exosomes is inconvenient to limit a centrifugal cylinder when in use.
In order to solve the technical problems, the utility model provides the following technical scheme: the ultrafiltration device special for extracting and separating the exosomes comprises a fixed table, a centrifugal cylinder and a motor, wherein the centrifugal cylinder is arranged on one side of the top end of the fixed table, a clamping structure is arranged on the outer side of the centrifugal cylinder, and the motor is arranged on the other side of the top end of the fixed table;
the bottom of motor is connected with the outside of a centrifugal tube through the belt, the bottom of a centrifugal tube is provided with the connecting pipe, the inside of connecting pipe is provided with filtration, one side of connecting pipe bottom is provided with the collecting box, the opposite side of connecting pipe bottom is provided with draws the section of thick bamboo, the top of drawing the section of thick bamboo is provided with the cylinder, the inside of drawing the section of thick bamboo is provided with the milipore filter, one side of drawing the section of thick bamboo is provided with the discharge gate, the clamping structure includes bracing piece, holder, movable groove and movable pearl, the outside of a centrifugal tube is located to the holder cover.
Preferably, the movable groove is formed in the inner side of the clamping frame, movable beads are arranged in the movable groove, and support rods are arranged on two sides of the clamping frame.
Preferably, the support rods are provided with two groups, and the two groups of support rods are symmetrically distributed on two sides of the clamping frame.
Preferably, the inner diameter of the movable groove is larger than the outer diameter of the movable bead, and the movable groove is movably connected with the movable bead.
Preferably, the movable beads are provided in a plurality, and the movable beads are distributed in a ring shape on the inner side of the clamping frame.
Preferably, the inner diameter of the clamping frame is larger than the outer diameter of the centrifugal cylinder, and a building structure is formed between the clamping frame and the centrifugal cylinder.
Preferably, the filter structure comprises a mounting frame, a filter screen and filter cotton, wherein the mounting frame is arranged in the connecting pipe, the filter screen is arranged in the mounting frame, and the filter cotton is arranged on one side of the filter screen.
The ultrafiltration device special for extracting and separating exosomes has the advantages that: through being provided with the clamping structure, in order to make the centrifugal barrel more stable when rotatory, consequently through the cooperation use of bracing piece, grip bracket, movable groove and movable pearl, can carry out a centre gripping to the centrifugal barrel, make the centrifugal barrel more stable when rotatory to the effect of centre gripping has been improved;
through being provided with filtration, in order to give the impurity in the exosome and filter, consequently through the cooperation use of filter screen and filter pulp, can carry out a multistage filtration to the exosome, reduce the impurity in the exosome, make the exosome impurity less after extracting to filterable effect has been improved.
Drawings
In order to more clearly illustrate the embodiments of the present utility model or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic elevational view of the present utility model;
FIG. 2 is a schematic top view of the present utility model;
FIG. 3 is a schematic view of the structure of the present utility model shown in FIG. 1 at a partially enlarged scale;
FIG. 4 is a schematic cross-sectional elevation view of a clamping structure according to the present utility model;
fig. 5 is a schematic front perspective view of a clamping structure according to the present utility model.
Reference numerals in the drawings illustrate: 1. a fixed table; 2. a clamping structure; 201. a support rod; 202. a clamping frame; 203. a movable groove; 204. a movable bead; 3. a centrifugal barrel; 4. a motor; 5. a belt; 6. a cylinder; 7. an extraction cylinder; 8. a discharge port; 9. an ultrafiltration membrane; 10. a collection box; 11. a connecting pipe; 12. a filtering structure; 1201. a mounting frame; 1202. a filter screen; 1203. and (5) filtering cotton.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. All other embodiments, which can be made by one of ordinary skill in the art without inventive faculty, are intended to be within the scope of the present utility model, based on the embodiments of the present utility model.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Example 1
Referring to fig. 1-5, the ultrafiltration device special for extracting and separating exosomes provided by the utility model comprises a fixed table 1, a centrifugal cylinder 3 and a motor 4, wherein the centrifugal cylinder 3 is arranged on one side of the top end of the fixed table 1, a clamping structure 2 is arranged on the outer side of the centrifugal cylinder 3, and the motor 4 is arranged on the other side of the top end of the fixed table 1;
the bottom of motor 4 is connected with the outside of centrifugal section of thick bamboo 3 through belt 5, the bottom of centrifugal section of thick bamboo 3 is provided with connecting pipe 11, the inside of connecting pipe 11 is provided with filtration 12, one side of connecting pipe 11 bottom is provided with collecting box 10, the opposite side of connecting pipe 11 bottom is provided with draws section of thick bamboo 7, the top of drawing section of thick bamboo 7 is provided with cylinder 6, the inside of drawing section of thick bamboo 7 is provided with milipore filter 9, one side of drawing section of thick bamboo 7 is provided with discharge gate 8, filtration 12 includes mounting bracket 1201, filter screen 1202, filter cotton 1203, mounting bracket 1201 sets up in the inside of connecting pipe 11, the inside of mounting bracket 1201 is provided with filter screen 1202, one side of filter screen 1202 is provided with filter cotton 1203.
The working principle of the ultrafiltration device special for extracting and separating exosomes based on the embodiment 1 is as follows: when extracting the exosome, pour the inside of centrifugal section of thick bamboo 3 into with the raw materials, pour into later start motor 4, motor 4 can drive centrifugal section of thick bamboo 3 through belt 5 and carry out high-speed rotation after the start, thereby give the separation with the inside impurity of exosome through centrifugal force, stop centrifugal section of thick bamboo 3 rotation after separating, then open the valve of centrifugal section of thick bamboo 3 bottom, make the exosome raw materials flow to the inside of connecting pipe 11, at this moment filter screen 1202 and filter cotton 1203's use, can carry out a filtration to the exosome, give the impurity of its inside and filter down, the impurity that filters down falls to the inside of collecting box 10, thereby accomplish the filtration work, the liquid that filters down can flow to the inside of extracting section of thick bamboo 7, then start cylinder 6, cylinder 6 can extrude the piston at the inside of extracting section of thick bamboo 7 after the start, thereby carry out an extrusion to exosome liquid, at this moment can carry out a filtration to the exosome, make water fall to the inside bottom of extracting section of thick bamboo 7, the exosome flows out through discharge gate 8, thereby accomplish the extraction separation work of exosome.
Example two
The embodiment further includes: the clamping structure 2 comprises supporting rods 201, clamping frames 202, movable grooves 203 and movable beads 204, wherein the clamping frames 202 are sleeved on the outer side of the centrifugal cylinder 3, the movable grooves 203 are formed in the inner side of the clamping frames 202, the movable beads 204 are arranged in the movable grooves 203, the supporting rods 201 are arranged on two sides of the clamping frames 202, the supporting rods 201 are arranged in two groups, the two groups of supporting rods 201 are symmetrically distributed on two sides of the clamping frames 202, the inner diameter of each movable groove 203 is larger than the outer diameter of each movable bead 204, the movable grooves 203 are movably connected with the movable beads 204, a plurality of movable beads 204 are arranged on the inner side of the clamping frames 202 in an annular mode, the inner diameter of each clamping frame 202 is larger than the outer diameter of the centrifugal cylinder 3, and a set of structure is formed between the clamping frames 202 and the centrifugal cylinder 3.
In this embodiment, the use of the clamping frame 202 can limit the centrifugal barrel 3 when the centrifugal barrel 3 rotates, so that the centrifugal barrel 3 is more stable when rotating, and the friction between the clamping frame 202 and the centrifugal barrel 3 is reduced by the cooperation of the movable groove 203 and the movable bead 204, so that the rotation of the centrifugal barrel 3 is not affected by the clamping frame 202 in the process of limiting and clamping, and the limiting operation is completed.
In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
The apparatus embodiments described above are merely illustrative, wherein the elements illustrated as separate elements may or may not be physically separate, and the elements shown as elements may or may not be physical elements, may be located in one place, or may be distributed over a plurality of network elements. Some or all of the modules may be selected according to actual needs to achieve the purpose of the solution of this embodiment. Those of ordinary skill in the art will understand and implement the present utility model without undue burden.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims (7)
1. The utility model provides a special ultrafiltration device of exosome extraction separation, includes fixed station (1), centrifugal tube (3) and motor (4), its characterized in that: a centrifugal cylinder (3) is arranged on one side of the top end of the fixed table (1), a clamping structure (2) is arranged on the outer side of the centrifugal cylinder (3), and a motor (4) is arranged on the other side of the top end of the fixed table (1);
the bottom of motor (4) is connected with the outside of a centrifugal cylinder (3) through belt (5), the bottom of a centrifugal cylinder (3) is provided with connecting pipe (11), the inside of connecting pipe (11) is provided with filtration (12), one side of connecting pipe (11) bottom is provided with collecting box (10), the opposite side of connecting pipe (11) bottom is provided with draws section of thick bamboo (7), the top of drawing section of thick bamboo (7) is provided with cylinder (6), the inside of drawing section of thick bamboo (7) is provided with milipore filter (9), one side of drawing section of thick bamboo (7) is provided with discharge gate (8), filtration (2) include bracing piece (201), carriage (202), movable groove (203) and movable bead (204), the outside of centrifugal cylinder (3) is located to carriage (202) cover.
2. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the movable groove (203) is formed in the inner side of the clamping frame (202), movable beads (204) are arranged in the movable groove (203), and supporting rods (201) are arranged on two sides of the clamping frame (202).
3. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the two groups of the supporting rods (201) are symmetrically distributed on two sides of the clamping frame (202).
4. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the inner diameter of the movable groove (203) is larger than the outer diameter of the movable bead (204), and the movable groove (203) is movably connected with the movable bead (204).
5. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the movable beads (204) are arranged in a plurality, and the movable beads (204) are distributed in an annular mode on the inner side of the clamping frame (202).
6. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the inner diameter of the clamping frame (202) is larger than the outer diameter of the centrifugal barrel (3), and a building structure is formed between the clamping frame (202) and the centrifugal barrel (3).
7. The exosome extraction and separation special ultrafiltration device according to claim 1, wherein: the filter structure (12) comprises a mounting frame (1201), a filter screen (1202) and filter cotton (1203), wherein the mounting frame (1201) is arranged in the connecting pipe (11), the filter screen (1202) is arranged in the mounting frame (1201), and the filter cotton (1203) is arranged on one side of the filter screen (1202).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321631290.0U CN220125701U (en) | 2023-06-26 | 2023-06-26 | Special ultrafiltration device for extracting and separating exosomes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321631290.0U CN220125701U (en) | 2023-06-26 | 2023-06-26 | Special ultrafiltration device for extracting and separating exosomes |
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| CN220125701U true CN220125701U (en) | 2023-12-05 |
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| CN202321631290.0U Active CN220125701U (en) | 2023-06-26 | 2023-06-26 | Special ultrafiltration device for extracting and separating exosomes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117720993A (en) * | 2024-02-07 | 2024-03-19 | 山东善缘生物科技有限公司 | Stem cell exosome extractor |
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2023
- 2023-06-26 CN CN202321631290.0U patent/CN220125701U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117720993A (en) * | 2024-02-07 | 2024-03-19 | 山东善缘生物科技有限公司 | Stem cell exosome extractor |
| CN117720993B (en) * | 2024-02-07 | 2024-05-07 | 山东善缘生物科技有限公司 | Stem cell exosome extractor |
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