CN117720993B - Stem cell exosome extractor - Google Patents
Stem cell exosome extractor Download PDFInfo
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- CN117720993B CN117720993B CN202410171820.0A CN202410171820A CN117720993B CN 117720993 B CN117720993 B CN 117720993B CN 202410171820 A CN202410171820 A CN 202410171820A CN 117720993 B CN117720993 B CN 117720993B
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- 210000001808 exosome Anatomy 0.000 title claims abstract description 38
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 25
- 238000000605 extraction Methods 0.000 claims abstract description 68
- 238000000926 separation method Methods 0.000 claims abstract description 38
- 238000001914 filtration Methods 0.000 claims abstract description 35
- 238000007790 scraping Methods 0.000 claims abstract description 28
- 239000012528 membrane Substances 0.000 claims abstract description 21
- 238000004140 cleaning Methods 0.000 claims abstract description 14
- 238000005192 partition Methods 0.000 claims description 20
- 230000000694 effects Effects 0.000 abstract description 12
- 230000000903 blocking effect Effects 0.000 abstract description 4
- 210000002421 cell wall Anatomy 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 14
- 239000012153 distilled water Substances 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 241000894006 Bacteria Species 0.000 description 10
- 238000005119 centrifugation Methods 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000001681 protective effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000005199 ultracentrifugation Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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Abstract
The invention relates to the technical field of exosome extractors, and provides a stem cell exosome extractor, which comprises a base, wherein a driving mechanism is arranged at the inner bottom end of the base, an extraction and separation mechanism is arranged inside the inner cylinder, and a scraping mechanism and a watertight cleaning mechanism are arranged inside the extraction and separation mechanism; the middle part of the second supporting plate is rotationally connected with an extraction pipe, a plurality of filtering membranes I are fixedly connected to the periphery of the first separating cylinder, a second separating cylinder is fixedly connected to the lower portion of the inner cylinder, a plurality of filtering membranes II are fixedly connected to the periphery of the separating cylinder, a drainage pipe is fixedly connected to the bottom end of the inner cylinder, and a valve is arranged on the outer side of the drainage pipe. According to the invention, through the arrangement of the driving mechanism, the extraction and separation mechanism, the scraping mechanism and the watertight cleaning mechanism, separation and extraction of exosomes can be completed in a sterile environment, and meanwhile, the influence on the filtering effect caused by blocking corresponding filtering pieces by cell walls can be avoided, and in addition, the device can be automatically flushed after the exosomes are taken away.
Description
Technical Field
The invention relates to the technical field of exosome extractors, in particular to a stem cell exosome extractor.
Background
The exosomes are disc-shaped vesicles with diameters of 40-100 nanometers, and generally contain complex RNA, protein, nucleic acid, lipid and the like, so that when the cells release the exosomes, the exosomes can flow between the cells through extracellular matrixes, and the information transmission effect can be achieved through the combination of related substances.
Usually, the extraction of exosomes is mainly realized by an ultracentrifugation method, but the ultracentrifugation method can obtain required substances through multiple times of centrifugation, and substances can be dispersed due to touch and vibration when sampling is performed after each centrifugation, so that the centrifugal effect is affected, the extraction difficulty is increased, meanwhile, the condition that the exosomes are polluted by external bacteria can occur in the extraction, the risk is increased through multiple times of centrifugation extraction, the extraction quality of the exosomes can be reduced, in addition, staff can be busy with the preservation and analysis of the extracts during actual use, the extraction device can not be cleaned in time under partial conditions, and bacteria can be bred to affect the subsequent extraction.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a stem cell exosome extractor, which solves the problems that the quality of the extract is reduced due to the influence of external bacteria, and the subsequent use is influenced due to the possibility of bacteria breeding in the device under partial conditions.
In order to achieve the above purpose, the invention is realized by the following technical scheme: the stem cell exosome extractor comprises a base, wherein a driving mechanism is arranged at the inner bottom end of the base, an outer shell is arranged at the top end of the driving mechanism, the outer side of the outer shell is arranged inside the base, an inner cylinder is connected inside the outer shell in a sliding manner, an extraction and separation mechanism is arranged inside the inner cylinder, and a scraping mechanism and a watertight cleaning mechanism are arranged inside the extraction and separation mechanism;
The utility model provides a draw separating mechanism, including the backup pad one that the level set up, backup pad one outside fixedly connected with is in inner tube upper portion, backup pad one middle part fixedly connected with backup pad two, backup pad two inside front sides are provided with the rubber buffer, backup pad two middle parts rotate and are connected with the extraction tube, middle part fixedly connected with separating tube one in the inner tube, a plurality of filtration membrane one of periphery fixedly connected with of separating tube, lower part fixedly connected with separating tube two in the inner tube, a plurality of filtration membrane two of separating tube periphery fixedly connected with, separating tube one with separating tube two are round platform shape, separating tube one through filtration membrane one with separating tube two intercommunication, the extraction tube lower part runs through separating tube one, the extraction tube bottom rotates to be connected with in separating tube two inner bottom, a plurality of notches have been seted up to the extraction tube bottom, inner tube bottom fixedly connected with drainage tube, the sliding connection has a protective tube in the outer tube, bottom fixedly connected with in the shell, shell body bottom fixedly connected with blow off pipe, the valve top is provided with valve in the middle part, the valve is fixedly connected with in the bottom at the base.
Preferably, the driving mechanism comprises a servo motor, the rear part of the bottom end of the base is fixedly connected with the servo motor, the output end of the servo motor penetrates through the base and is fixedly connected with a first gear, the first front part of the gear is meshed with a second gear, the top end of the second gear is fixedly connected with the middle part of the bottom end of the outer shell, the outer part of the bottom end of the base is fixedly connected with a plurality of supporting columns, the top ends of the supporting columns are all rotationally connected with balls, and the top ends of the balls are all rotationally connected with the outer part of the bottom end of the outer shell.
Preferably, the scraping mechanism comprises a rotating ring, the inner side of the rotating ring is fixedly connected to the upper portion of the outer side of the extraction tube, a plurality of T-shaped plates are fixedly connected to the outer side of the rotating ring, a first scraping plate is fixedly connected to the middle of the extraction tube, one end of the first scraping plate, which is far away from the extraction tube, is slidably connected to the inside of the first separating tube, a second scraping plate is fixedly connected to the lower portion of the extraction tube, and one end of the second scraping plate, which is far away from the extraction tube, is slidably connected to the inside of the second separating tube.
Preferably, the watertight cleaning mechanism comprises an annular partition plate, annular partition plate bottom fixed connection is close to two sides of a supporting plate, annular partition plate lower part sliding connection has a movable storehouse, a plurality of sliding columns of inside sliding connection in two sides of a supporting plate are kept away from to the first supporting plate, a plurality of J-shaped holes have all been seted up on sliding column upper portion, a plurality of sliding column bottom equal fixedly connected with spring one, a plurality of spring one bottom equal fixedly connected with is on a separating drum top, inner tube inner bottom fixedly connected with fixed plate, fixed plate top fixedly connected with spring two, two top fixedly connected with fretwork disc, fretwork disc top middle part fixedly connected with a plurality of connecting rods, a plurality of connecting rod lower part outside equal fixedly connected with lug one, a plurality of lug one outside equal sliding connection is in two inner bottom middle parts of separating drum, a plurality of support rods are connected with each other in a plurality of lug two outside at one inner bottom middle parts of separating drum, fretwork disc top outside fixedly connected with a plurality of support rods, a plurality of electric push rod outside equal fixedly connected with three outer side of the same three outer side of the front side of the outer casing of the electric push rod.
Preferably, the first end of the gear is externally connected with the first support frame in a rotating manner, the first end of the support frame is fixedly connected with the inner bottom end of the base, the second end of the gear is connected with the second support frame in a rotating manner, and the second end of the support frame is fixedly connected with the inner bottom end of the base.
Preferably, the upper part in the base is fixedly connected with a rotating frame, and the inner side of the rotating frame is connected with the outer side of the shell in a sliding manner.
Preferably, the two sides of the top end of the fixing plate are fixedly connected with first limiting rods, the upper parts of the first limiting rods respectively penetrate through the two sides of the hollowed-out disc, and the top ends of the first limiting rods are respectively and fixedly connected with the two sides of the two bottom ends of the separating cylinder.
Preferably, the lower part in a plurality of sliding columns is all sliding connection has the gag lever post two, and a plurality of gag lever post two bottom is all fixed connection be in separating tube one top, a plurality of spring one is all twined gag lever post two outsides, gag lever post two with three dislocation sets of lug.
Preferably, the upper end and the lower end of the side, far away from the second support plate, of the movable bin are fixedly connected with convex plates, and the bottom end of the movable bin is slidably connected to the top end of the second support plate.
Preferably, a plurality of dogs are fixedly connected to the lower part of the inner side of the outer shell, and the top ends of the dogs are all abutted to the bottom end of the inner cylinder.
Working principle: when in use, the stem cell solution containing exosome is injected into the first separating cylinder through the rubber plug, so that the air pressure in the space below the first supporting plate and the second supporting plate can be increased, the sliding column can overcome the limit of the first spring and move upwards at the moment, so that distilled water in the space formed by the annular partition plate and the inner cylinder can push the movable cabin to be close to the rubber plug, after the injection is finished, the blocking of the movable cabin is released, the movable cabin can shield the rubber plug under the pushing of the internal pressure of distilled water, external bacteria are prevented from entering the area below the supporting plate through the through hole left by the injector, the influence of the bacteria on the exosome quality can be reduced, the servo motor is started, the first gear can transmit the rotation output by the servo motor to the outer shell through the second gear, and the outer shell is connected with the inner cylinder through the groove and the convex strip, so that the inner cylinder can synchronously rotate along with the outer shell, at the moment, stem cell solution in the inner cylinder can be separated under the action of centrifugal force, so that cell fragments and large-particle outer vesicles can be blocked by the first filtering film, and exosomes, solution and the like can pass through the first filtering film and fall into the second separating cylinder, so that exosomes can be blocked by the second filtering film and stay in the second separating cylinder, other small-particle molecules can pass through the second filtering film, thereby realizing aseptic extraction of exosomes, reducing the time spent by multistage extraction and the probability of bacterial pollution, and the extraction pipe can not synchronously rotate along with the inner cylinder through the components of the extraction and separation mechanism, and simultaneously the stability and the self weight of the extraction pipe can be further increased through the T-shaped plate and the rotating ring, therefore, when the inner cylinder rotates, other components of the extraction separation mechanism which synchronously rotates with the inner cylinder can have a certain speed difference with the extraction pipe, and as the scraping plate I and the scraping plate II are connected with the extraction pipe, the scraping plate I and the scraping plate II can respectively move along the separation cylinder I and the separation cylinder II under the action of the speed difference, so as to scrape the filtering membrane I and the filtering membrane II, the poor separation effect caused by the blockage of the filtering membrane I and the filtering membrane II is avoided, the servo motor is closed after the separation is finished, at the moment, the inner cylinder can be lifted along the groove in the outer shell under the pushing of the electric push rod, the air pressure of the area between the outer shell and the inner cylinder can be gradually reduced along with the lifting of the inner cylinder due to the closing state of the valve, when the inner cylinder moves for a certain distance, a worker pulls out the plug at the top of the extraction pipe, and then the injector extends into the separation cylinder II along the extraction pipe to extract the separated exosome body, when the extraction is finished, the plug is plugged back, the inner cylinder is continuously pushed to move upwards by the electric push rod, the lower negative pressure is gradually larger than the bearable range of the second spring and the first spring, so that the second spring and the first spring can be stressed to shrink, the sliding column can move downwards, distilled water on the inner side of the annular partition plate can enter the first separation cylinder through the J-shaped hole in the sliding column to flush the inner part of the first separation cylinder, and the first projection, the second projection and the third projection connected with the connecting rod and the supporting rod can move downwards simultaneously by the hollow disc when the second spring shrinks, so that distilled water entering the first separation cylinder can sequentially enter the inner space of the second separation cylinder and the drainage tube through the first projection, the second projection and the third projection after the flushing is finished, and then the distilled water can be discharged along the drain pipe after the valve is opened to finish cleaning, the device is prevented from breeding bacteria due to untimely cleaning, and further the influence on the next extraction can be avoided.
The invention provides a stem cell exosome extractor. The beneficial effects are as follows:
According to the invention, the drive mechanism is arranged to enable the extraction and separation mechanism, the scraping mechanism and the watertight cleaning mechanism to exert effects, so that separation and extraction of exosomes can be conveniently completed in a sterile environment, repeated centrifugation is not needed, the working flow is simplified, the influence on the filtering effect caused by blocking corresponding filtering pieces by cell walls can be avoided, and in addition, the interior of the device can be automatically flushed after the exosomes are taken away, so that the influence on the subsequent extraction effect caused by bacteria breeding by the device is avoided.
Drawings
FIG. 1 is a front perspective view of the present invention;
FIG. 2 is a bottom perspective view of the present invention;
FIG. 3 is a bottom perspective cross-sectional view of the present invention;
FIG. 4 is an enlarged view of FIG. 3 at A;
FIG. 5 is a side perspective partial cross-sectional view of the present invention;
FIG. 6 is an enlarged view of FIG. 5at B;
FIG. 7 is a perspective view of an inner barrel connecting structure of the present invention;
FIG. 8 is an enlarged view of FIG. 7 at C;
fig. 9 is an enlarged view of D in fig. 7.
1, A base; 2. a driving mechanism; 201. a servo motor; 202. a first gear; 203. a second gear; 204. a support column; 205. a ball; 206. a first supporting frame; 207. a second supporting frame; 208. a rotating frame; 3. an outer housing; 4. an inner cylinder; 5. an extraction and separation mechanism; 501. a first supporting plate; 502. a second supporting plate; 503. a rubber stopper; 504. an extraction tube; 505. a first separation cylinder; 506. a first filtering membrane; 507. a second separating cylinder; 508. a second filtering membrane; 509. a drainage tube; 510. a protective cylinder; 511. a blow-down pipe; 512. a valve; 6. a scraping mechanism; 601. a rotating ring; 602. a T-shaped plate; 603. a first scraping plate; 604. a second scraping plate; 7. a watertight cleaning mechanism; 701. an annular partition plate; 702. a movable bin; 703. a sliding column; 704. a J-shaped aperture; 705. a first spring; 706. a fixing plate; 707. a second spring; 708. hollow discs; 709. a connecting rod; 710. a first bump; 711. a second bump; 712. a support rod; 713. a third bump; 714. an electric push rod; 715. a first limit rod; 716. a second limiting rod; 717. a convex plate.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples:
Referring to fig. 1-9, an embodiment of the present invention provides a stem cell exosome extractor, including a base 1, a driving mechanism 2 is disposed at an inner bottom of the base 1, an outer casing 3 is disposed at a top end of the driving mechanism 2, an outer side of the outer casing 3 is disposed inside the base 1, an inner cylinder 4 is slidably connected inside the outer casing 3, an extraction separation mechanism 5 is disposed inside the inner cylinder 4, and a scraping mechanism 6 and a watertight cleaning mechanism 7 are disposed inside the extraction separation mechanism 5;
The extraction separation mechanism 5 comprises a first supporting plate 501 which is horizontally arranged, the outer side of the first supporting plate 501 is fixedly connected to the inner upper portion of the inner cylinder 4, a second supporting plate 502 is fixedly connected to the middle portion of the first supporting plate 501, a rubber plug 503 is arranged at the front side inside the second supporting plate 502, an extraction pipe 504 is rotatably connected to the middle portion of the second supporting plate 502, a first separating cylinder 505 is fixedly connected to the inner middle portion of the inner cylinder 4, a plurality of filtering membranes 506 are fixedly connected to the outer periphery of the first separating cylinder 505, a second separating cylinder 507 is fixedly connected to the inner lower portion of the inner cylinder 4, a plurality of filtering membranes 508 are fixedly connected to the outer periphery of the second separating cylinder 507, the first separating cylinder 505 and the second separating cylinder 507 are in a circular truncated cone shape, the first separating cylinder 505 is communicated with the second separating cylinder 507 through the first filtering membranes 506, the lower portion of the extraction pipe 504 penetrates the first separating cylinder 505, the bottom end of the extraction pipe 504 is rotatably connected to the inner bottom end of the second separating cylinder 507, a plurality of notches are formed in the bottom end of the extraction pipe 504, a drainage pipe 509 is fixedly connected to the bottom of the inner cylinder 509, a protective cylinder 510 is slidably connected to the outer side of the drainage pipe 509, the bottom of the protective cylinder 510 is fixedly connected to the bottom of the outer casing 3, a valve 512 is fixedly connected to the bottom the outer casing 511, a valve 512 is provided to the top the valve 512, the valve 512 is fixedly connected to the valve 512 is fixedly to the bottom the valve 512, and is fixedly connected to the bottom the valve 512 is connected to the bottom the valve base 1.
The inner cylinder 4 can be separated from the outside through the first supporting plate 501 and the second supporting plate 502, so that the stem cell solution is prevented from being directly influenced by bacteria, the stem cell solution can be conveniently injected into the first separating cylinder 505 through the rubber plug 503 at the eccentric position of the second supporting plate 502, the outer shell 3 can drive the inner cylinder 4 to rotate under the action of the driving mechanism 2, and then the stem cell solution can be separated under the action of centrifugal force, and the inner spaces of the inner cylinders 4 divided by the circular truncated cone-shaped separating cylinder 505 and the separating cylinder two 507 are communicated through the first filtering film 506 and the second filtering film 508, so that large particle substances such as cell fragments, large particle outer vesicles and the like in the separated stem cell solution can be reserved in the first separating cylinder 505, and exosome and related solutions can enter the second separating cylinder 507 through the first filtering film 506, so that the solution can pass through the second filtering film 508 under the action of the centrifugal force, the exosome is blocked in the second separating cylinder 507, the extraction of the related solutions can be prevented from being repeatedly extracted from being contacted with the outside environment when the multistage centrifugal force is avoided, and the quality of the extract is prevented from being jointly blocked by the second separating cylinder 504 under the action of the extracting cylinder, and the quality of the exosome is prevented from being taken out from being jointly separated under the action of the second separating cylinder 504.
The driving mechanism 2 comprises a servo motor 201, the rear part of the bottom end of the base 1 is fixedly connected with the servo motor 201, the output end of the servo motor 201 penetrates through the base 1 and is fixedly connected with a first gear 202, a second gear 203 is meshed with the front part of the first gear 202, the top end of the second gear 203 is fixedly connected with the middle part of the bottom end of the outer shell 3, a plurality of support columns 204 are fixedly connected with the outer part of the bottom end of the base 1, the top ends of the support columns 204 are all rotationally connected with balls 205, and the top ends of the balls 205 are all rotationally connected with the outer part of the bottom end of the outer shell 3.
The first gear 202 can be rotated through the servo motor 201, the second gear 203 can further enable the outer shell 3 to drive the inner cylinder 4 to rotate so as to meet the centrifugal requirement, and the first gear 202 is larger than the second gear 203 in diameter, so that the rotating speed of the second gear 203 can be further improved, and the balls 205 can be supported through the supporting columns 204, so that the balls 205 can play a role in supporting the outer shell 3, friction force between the outer shell 3 and the base 1 can be reduced, and energy loss is avoided.
The scraping mechanism 6 comprises a rotating ring 601, the inner side of the rotating ring 601 is fixedly connected to the upper portion of the outer side of the extraction tube 504, a plurality of T-shaped plates 602 are fixedly connected to the outer side of the rotating ring 601, a first scraping plate 603 is fixedly connected to the middle of the extraction tube 504, one end of the first scraping plate 603, far away from the extraction tube 504, is slidably connected to the inside of the first separation tube 505, a second scraping plate 604 is fixedly connected to the lower portion of the extraction tube 504, and one end, far away from the extraction tube 504, of the second scraping plate 604 is slidably connected to the inside of the second separation tube 507.
The dead weight and stability of the extraction pipe 504 can be increased through the uniformly distributed T-shaped plates 602 and the rotating ring 601, so that a certain speed difference can exist between the extraction pipe 504 and the inner cylinder 4 when the extraction pipe 504 rotates, and the scraper blade I603 and the scraper blade II 604 which synchronously rotate with the extraction pipe 504 can respectively move along the separation cylinder I505 and the separation cylinder II 507, so that the filtering membrane I506 and the filtering membrane II 508 can be scraped respectively, the filtering effect is prevented from being influenced due to the blocking of large particulate matters when the filtering membrane I506 and the filtering membrane II 508 are used, and further the filtering time is prevented from being prolonged.
The watertight cleaning mechanism 7 comprises an annular partition 701, the bottom end of the annular partition 701 is fixedly connected to one side, close to a second supporting plate 502, of the top end of the first supporting plate 501, the lower portion of the annular partition 701 is slidably connected with a movable bin 702, one side, far away from the second supporting plate 502, of the first supporting plate 501 is internally slidably connected with a plurality of sliding columns 703, a plurality of J-shaped holes 704 are formed in the upper portion of each sliding column 703, springs 705 are fixedly connected to the bottom ends of the sliding columns 703, the bottom ends of the springs 705 are fixedly connected to the top ends of the first 505 of the separating cylinders, the inner bottom ends of the inner cylinders 4 are fixedly connected with a fixing plate 706, the top ends of the fixing plate 706 are fixedly connected with springs 707, the top ends of the springs 707 are fixedly connected with hollow discs 708, the middle portions of the hollow discs 708 are fixedly connected with a plurality of connecting rods 709, the outer sides of the lower portions of the connecting rods 709 are fixedly connected with lugs 710, the outer sides of the lugs 710 are slidably connected to the middle portions of the inner bottom ends of the second 507 of the separating cylinders, the outer sides of the lugs 711 are fixedly connected to the middle portions of the inner bottom ends of the first 505 of the separating cylinders, the outer sides of the hollow discs 708 are fixedly connected to the outer sides of the outer casing 713, the outer sides of the outer casing is fixedly connected to the front ends of the outer casing 3, and the outer sides of the outer casing is fixedly connected to the front ends of the outer sides of the outer casing 3.
An annular closed space is formed by the annular partition 701, the inner cylinder 4 and the first support plate 501 to accommodate distilled water, and the valve 512 is closed before centrifugation, so that the air pressure in the inner cylinder 4 is not leaked, the J-shaped hole 704 in the sliding column 703 is positioned at the upper part of the first support plate 501 by the first spring 705 to avoid the distilled water directly flowing into the first separation cylinder 505 to affect normal separation, before the stem cell solution is injected, a worker needs to prop against the movable cabin 702 by hands or other objects, the air pressure in the inner cylinder 4 can be raised along with the injection of the stem cell solution, at this time, the balance between the first spring 705 and the sliding column 703 can be broken, so that the sliding column 703 can move upwards, and the pressure of the distilled water in the annular partition 701 can be increased, so that the distilled water can push the movable cabin 702 to have a movement trend of leaving the inner space of the annular partition 701, when the stem cell solution is injected, the worker does not block the movable bin 702 any more, at this time, the movable bin 702 can move to the upper side of the rubber plug 503 under the pressure effect to block the hole left when the injector is used, bacteria are prevented from entering the inner cylinder 4 along the hole to contact with the stem cell solution, when the separation is finished, the inner cylinder 4 can be upwards moved through the electric push rod 714, the worker can conveniently pull out the plug at the top of the extraction pipe 504 to extract the exosome in the second separation pipe 507 through the injector, when the exosome is extracted, the plug is plugged into the extraction pipe 504 again, and another plug at the top of the annular partition 701 is pulled out, so that the inside of the annular partition 701 can be communicated with the atmosphere, and the electric push rod 714 continues to push the inner cylinder 4 to move upwards, at this time, the lower part of the inner cylinder 4 is in a sealing state, therefore, as the space between the bottom of the inner cylinder 4 and the outer shell 3 is gradually increased, the air pressure is gradually reduced, after the inner cylinder 4 moves a certain distance, the negative pressure value is larger than the bearing range of the first spring 705 and the second spring 707, so that the hollow disc 708 can drive the connecting rod 709 and the supporting rod 712 to move downwards, the first bump 710, the second bump 711 and the third bump 713 can move downwards along with the connecting rod, simultaneously the sliding column 703 can overcome the elasticity of the first spring 705 under the action of the negative pressure, after the lower part of the J-shaped hole 704 enters the lower part of the supporting plate 501 along with the sliding column 703, distilled water in the annular partition 701 can be sprayed to the inner area of the first separating cylinder 505 under the action of the negative pressure through the J-shaped hole 704, the purpose of cleaning the inner space of the first separating cylinder 505 is achieved, and the distilled water can continuously enter the second separating cylinder 507 through the through grooves left after the first bump 710, the second bump 711 and the third bump 713, and can flow into the drain 511 through the 509 and the protecting cylinder 510 after the second separating cylinder 507 is completely washed, the valve 512 can overcome the elasticity of the first spring 705, the lower part can drain the sliding column, after the valve 512 is opened, the distilled water can drain from the drain down, the relevant impurities can be drained from the drain down, the relevant structures can be avoided, the influence on the drain structure is avoided, and the inner cleaning is avoided, and the drain is avoided.
The outer rotation of gear one 202 bottom is connected with support frame one 206, and support frame one 206 bottom fixed connection is in base 1 inner bottom, and gear two 203 bottom rotates and is connected with support frame two 207, and support frame two 207 bottom fixed connection is in base 1 inner bottom.
The first gear 202 and the second gear 203 can be respectively supported by the first support frame 206 and the second support frame 207, so that the first gear 202 and the second gear 203 can be prevented from shaking when rotating, the first gear 202 and the second gear 203 can be ensured to be effectively meshed all the time, and the centrifugal effect is prevented from being influenced due to influence on transmission.
The upper part in the base 1 is fixedly connected with a rotating frame 208, and the inner side of the rotating frame 208 is connected with the outer side of the outer shell 3 in a sliding way.
The outer shell 3 can be limited through the rotating frame 208, so that the outer shell 3 can only rotate in situ, and further the upper part of the outer shell 3 can be prevented from shaking when the outer shell 3 rotates at a high speed, the stability of the outer shell 3 is improved, and the damage caused by overlarge stress of a related connecting structure is avoided, so that the purpose of protecting equipment is achieved.
The two sides of the top end of the fixing plate 706 are fixedly connected with a first limiting rod 715, the upper parts of the first limiting rod 715 respectively penetrate through the two sides of the hollowed-out disc 708, and the top ends of the first limiting rod 715 are respectively and fixedly connected with the two sides of the bottom end of the second separating cylinder 507.
The first limit rod 715 can limit the hollow disc 708, so that the first projection 710, the second projection 711 and the third projection 713 cannot return to the initial positions due to shaking of the hollow disc 708, and damage caused by excessive deformation of the second spring 707 can be prevented.
The lower part in a plurality of sliding columns 703 is all sliding connection has gag lever post two 716, and a plurality of gag lever post two 716 bottom all fixed connection is at the separating drum one 505 top, and a plurality of springs one 705 all twine in gag lever post two 716 outside, and gag lever post two 716 and bump three 713 dislocation set.
The top of the second limit rod 716 is connected with the sliding column 703 through the bulge, so that the sliding column 703 is prevented from falling off due to overlarge displacement, the sliding column 703 can further play a role stably, the first spring 705 is wound on the outer side of the second limit rod 716, the two limit rods 716 and the third bump 713 are prevented from being mutually influenced in action, and meanwhile, the effect of avoiding mutual interference in motion can be achieved due to dislocation of the second limit rod 716 and the third bump 713.
The upper end and the lower end of the side, far away from the second supporting plate 502, of the movable bin 702 are fixedly connected with convex plates 717, and the bottom end of the movable bin 702 is slidably connected to the top end of the second supporting plate 502.
The convex plate 717 can prevent the movable cabin 702 from being excessively displaced and completely separated from the range of the annular partition 701, so that the distilled water stored in the annular partition 701 can be prevented from leaking, and the movable cabin 702 can move along the surface of the second supporting plate 502, so that the movable cabin 702 can not form an excessive gap when shielding the rubber plug 503, and the sealing effect can be ensured.
The lower part of the inner side of the outer shell 3 is fixedly connected with a plurality of stop blocks, and the top ends of the stop blocks are all abutted to the bottom end of the inner cylinder 4.
The inner cylinder 4 can be supported by the uniformly distributed stoppers to avoid long-term stress of the electric push rod 714, and the electric push rod 714 can be protected when the inner cylinder 4 moves downwards to prevent the electric push rod 714 from being directly affected by impact force.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The stem cell exosome extractor comprises a base (1), and is characterized in that a driving mechanism (2) is arranged at the inner bottom end of the base (1), an outer shell (3) is arranged at the top end of the driving mechanism (2), the outer side of the outer shell (3) is arranged inside the base (1), an inner cylinder (4) is connected inside the outer shell (3) in a sliding manner, an extraction separation mechanism (5) is arranged inside the inner cylinder (4), a scraping mechanism (6) and a watertight cleaning mechanism (7) are arranged inside the extraction separation mechanism (5), a rotating frame (208) is fixedly connected to the inner upper part of the base (1), and the inner side of the rotating frame (208) is connected outside the outer shell (3) in a sliding manner.
The extraction separation mechanism (5) comprises a first supporting plate (501) which is horizontally arranged, the outer side of the first supporting plate (501) is fixedly connected with the inner upper part of an inner cylinder (4), the middle part of the first supporting plate (501) is fixedly connected with a second supporting plate (502), the front side of the inner part of the second supporting plate (502) is provided with a rubber plug (503), the middle part of the second supporting plate (502) is rotationally connected with an extraction pipe (504), the inner middle part of the inner cylinder (4) is fixedly connected with a first separating cylinder (505), the outer periphery of the first separating cylinder (505) is fixedly connected with a plurality of first filtering membranes (506), the inner lower part of the inner cylinder (4) is fixedly connected with a second separating cylinder (507), the outer periphery of the second separating cylinder (507) is fixedly connected with a plurality of second filtering membranes (508), the first separating cylinder (505) and the second separating cylinder (507) are all in a round table shape, the first separating cylinder (505) is communicated with the second separating cylinder (507) through the first filtering membranes (506), the lower part of the extracting pipe (504) penetrates through the first separating cylinder (505), the second bottom end (509) is rotationally connected with the second bottom end (509) and is fixedly connected with a plurality of drainage pipes (509), the bottom end of the protection cylinder (510) is fixedly connected to the inner bottom end of the outer shell (3), the bottom end of the outer shell (3) is fixedly connected with a drain pipe (511), a valve (512) is arranged on the outer side of the drain pipe (511), and the top end of the valve (512) is fixedly connected to the middle of the bottom end of the base (1);
the scraping mechanism (6) comprises a rotating ring (601), the inner side of the rotating ring (601) is fixedly connected to the upper portion of the outer side of the extraction tube (504), a plurality of T-shaped plates (602) are fixedly connected to the outer side of the rotating ring (601), a first scraping plate (603) is fixedly connected to the middle of the extraction tube (504), one end, far away from the extraction tube (504), of the first scraping plate (603) is slidably connected to the inside of the separation tube (505), a second scraping plate (604) is fixedly connected to the lower portion of the extraction tube (504), and one end, far away from the extraction tube (504), of the second scraping plate (604) is slidably connected to the inside of the separation tube (507);
The watertight cleaning mechanism (7) comprises an annular partition plate (701), the bottom end of the annular partition plate (701) is fixedly connected to one side, close to a second supporting plate (502), of the top end of the first supporting plate (501), a movable bin (702) is slidably connected to the lower portion of the annular partition plate (701), a plurality of sliding columns (703) are slidably connected to one side, far away from the second supporting plate (502), of the first supporting plate (501), a plurality of J-shaped holes (704) are formed in the upper portion of the sliding columns (703), springs I (705) are fixedly connected to the bottom ends of the sliding columns (703), a plurality of springs I (705) are fixedly connected to the bottom ends of the springs I (705) and are fixedly connected to the top end of a first separating cylinder (505), a fixed plate (706) is fixedly connected to the inner bottom end of the inner cylinder (4), a spring II (707) is fixedly connected to a hollow disc (708) on the top end of the inner cylinder, a plurality of connecting rods (709) are fixedly connected to the middle portion of the hollow disc (708), a plurality of protruding blocks (709) are fixedly connected to the outer sides of the connecting rods (709), a plurality of protruding blocks (710) are fixedly connected to the middle portions of the inner bottom ends (710), the outer sides of the convex blocks II (711) are all in sliding connection with the middle part of the inner bottom end of the first separating cylinder (505), the outer parts of the top ends of the hollow circular discs (708) are fixedly connected with a plurality of supporting rods (712), the top ends of the supporting rods (712) are all fixedly connected with a convex block III (713), the outer sides of the convex blocks III (713) are all in sliding connection with the outer part of the top end of the first separating cylinder (505), the front side and the rear side of the inner bottom end of the outer shell (3) are all fixedly connected with electric push rods (714), and the output ends of the electric push rods (714) are all fixedly connected with the bottom end of the inner cylinder (4).
2. The stem cell exosome extractor according to claim 1, wherein the driving mechanism (2) comprises a servo motor (201), the rear end of the bottom end of the base (1) is fixedly connected with the servo motor (201), the output end of the servo motor (201) penetrates through the base (1) and is fixedly connected with a first gear (202), the front part of the first gear (202) is meshed with a second gear (203), the top end of the second gear (203) is fixedly connected to the middle of the bottom end of the outer shell (3), a plurality of supporting columns (204) are fixedly connected to the outer bottom end of the base (1), the top ends of the supporting columns (204) are all rotationally connected with balls (205), and the top ends of the balls (205) are all rotationally connected to the outer bottom end of the outer shell (3).
3. The stem cell exosome extractor according to claim 2, wherein a first support frame (206) is rotatably connected to the outer bottom end of the first gear (202), the bottom end of the first support frame (206) is fixedly connected to the inner bottom end of the base (1), a second support frame (207) is rotatably connected to the bottom end of the second gear (203), and the bottom end of the second support frame (207) is fixedly connected to the inner bottom end of the base (1).
4. The stem cell exosome extractor according to claim 1, wherein two sides of the top end of the fixing plate (706) are fixedly connected with a first limit rod (715), the upper part of the first limit rod (715) respectively penetrates through two sides of the hollowed-out disc (708), and the top end of the first limit rod (715) is respectively fixedly connected with two sides of the bottom end of the second separation barrel (507).
5. The stem cell exosome extractor according to claim 1, wherein the lower parts in the sliding columns (703) are respectively and slidably connected with a second limiting rod (716), the bottom ends of the second limiting rods (716) are respectively and fixedly connected to the top ends of the first separating cylinders (505), the first springs (705) are respectively wound on the outer sides of the second limiting rods (716), and the second limiting rods (716) and the third protruding blocks (713) are arranged in a staggered manner.
6. The stem cell exosome extractor according to claim 1, wherein the upper end and the lower end of the side, away from the second supporting plate (502), of the movable bin (702) are fixedly connected with convex plates (717), and the bottom end of the movable bin (702) is slidably connected to the top end of the second supporting plate (502).
7. The stem cell exosome extractor according to claim 1, wherein a plurality of stoppers are fixedly connected to the lower portion of the inner side of the outer casing (3), and the top ends of the stoppers are abutted to the bottom end of the inner cylinder (4).
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