CN218507763U

CN218507763U - Exosome purification device

Info

Publication number: CN218507763U
Application number: CN202222624939.8U
Authority: CN
Inventors: 夏尼诺; 方芳; 李�杰
Original assignee: Hefei Hexin Biotechnology Co ltd
Current assignee: Hefei Hexin Biotechnology Co ltd
Priority date: 2022-09-30
Filing date: 2022-09-30
Publication date: 2023-02-21
Anticipated expiration: 2032-09-30

Abstract

The utility model provides an exosome purification device, include: the filter comprises a filter body, a first filter plate, a second filter plate and a third filter plate, wherein the first filter plate is positioned in the filter body; the lower pressing block is positioned in the separator, the lower end of the lower pressing block is attached to the upper surface of the lower fixing ring block, and the lower fixing ring block is welded on the outer surface of the separation vessel; and the centrifugal turntable is positioned in the separator, and the surface of the groove of the centrifugal turntable is attached to the lower end surface of the separation vessel. The utility model provides a when the centrifugal purification exosome, fast-speed rotation makes a large amount of exosomes gather the piece easily, and the purity of purification is not enough, influences the problem of exosome purification effect.

Description

Exosome purification device

Technical Field

The utility model relates to an exosome purification field, concretely relates to exosome purification device.

Background

The exosome refers to a small membrane vesicle containing complex RNA and protein, and refers to a disc-shaped vesicle with the diameter of 40-100nm nowadays, the exosome can be used as a natural carrying substance of a medicine, the exosome needs to be purified before use, and the exosome is usually purified in a centrifugal mode.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects in the prior art, the exosome purifying device is provided so as to solve the problems that when the exosome is centrifugally purified, a large amount of exosomes are easily aggregated into blocks by high-speed rotation, the purification purity is insufficient, and the purification effect of the exosome is influenced.

To achieve the above object, there is provided an exosome purification device comprising:

the filter comprises a first filter plate, a second filter plate and a third filter plate, wherein the first filter plate is positioned in the filter body;

the lower pressing block is positioned in the separator, the lower end of the lower pressing block is attached to the upper surface of the lower fixing ring block, and the lower fixing ring block is welded on the outer surface of the separation vessel;

and the centrifugal turntable is positioned in the separator, and the groove surface of the centrifugal turntable is attached to the lower end surface of the separation vessel.

Furthermore, a liquid inlet is formed in the surface of the upper end of the filter body, a liquid outlet is formed in the lower end of the filter body, and two groups of electric push rods are connected to the lower end of the filter body.

Furthermore, a door body is hinged to the surface of the front end of the filter body, and connecting blocks are welded on the left side wall and the right side wall of the separator; and an electric push rod is fixed on the upper surface of the connecting block.

Furthermore, a clamping and connecting plate is fixed inside the filter body; and the inside joint of joint inboard is connected with first filter plate, second filter plate and third filter plate respectively, separation household utensils upper end lateral wall welding has last fixed ring piece.

Furthermore, a concave arc-shaped filter membrane is arranged in the middle of the first filter plate, the second filter plate and the third filter plate, and four groups of plug-in columns are fixed on the upper surface of the centrifugal turntable; and the inserting column is inserted inside the lower end of the lower pressing block.

Furthermore, the upper end surface of the separator is connected with an upper cover in a pressing way, and the lower end of the centrifugal turntable is connected with a low-speed centrifugal motor through a centrifugal rotating shaft; and the low-speed centrifugal motor is fixed in the bottom of the separator.

Furthermore, the surface bearing of the upper end of the lower pressing block is connected with an adjusting stud; and the adjusting stud is connected with the upper end of the upper fixed ring block in a penetrating way, and the lower pressing block is connected with the upper fixed ring block through a compression spring.

The beneficial effects of the utility model reside in that, the utility model discloses an exosome purification device utilizes the inside three-layer filter plate membrane of filter organism, carry out ultrafiltration separation to exosome solution, the rapid filtration of being convenient for can't pass through the material in space, be convenient for effectual purification, ultrafiltration most exosomes, be convenient for ensure exosome's integrality and biological activity, rethread low-speed centrifugal motor drives separator ware centrifugation rotation, the exosome after making the inside ultrafiltration of separation household utensils carries out low-speed centrifugation, reduce rotation rate, avoid a large amount of exosomes to gather the manifold block, a small amount of exosomes carry out effectual separation under certain rotational speed and time simultaneously, the purification, ultrafiltration, centrifugation's dual purification, improve exosome's purification effect, the problem of influence exosome purification effect has been solved.

Drawings

Fig. 1 is a schematic front view of an exosome purification device according to an embodiment of the present invention.

Fig. 2 is a schematic view of a front-view cross-sectional structure of an exosome purification device according to an embodiment of the present invention.

Fig. 3 is a schematic view of an elevational view connecting structure of the separation vessel and the lower pressing block according to the embodiment of the present invention.

Fig. 4 is a schematic top view of the first filter plate according to the embodiment of the present invention.

1. Filtering the machine body; 11. a liquid inlet; 12. a door body; 13. a liquid discharge port; 14. an electric push rod; 15. a clamping and connecting plate; 2. a separator; 21. an upper cover; 22. a joining block; 3. a first filter plate; 31. a second filter plate; 32. a third filter plate; 33. an arc-shaped filter membrane; 4. separating the vessel; 41. an upper fixed ring block; 42. a lower stationary ring block; 5. pressing the block; 51. a compression spring; 52. adjusting the stud; 6. a centrifugal turntable; 61. inserting the column; 62. a centrifugal rotating shaft; 63. a low speed centrifugal motor.

Detailed Description

The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The present invention can be implemented or applied by other different specific embodiments, and various details in the present specification can be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

Fig. 1 is the utility model provides an embodiment of the front view structure sketch of exosome purification device, fig. 2 are the utility model discloses embodiment of the front view section structure sketch of exosome purification device, fig. 3 are the utility model discloses embodiment of the front view connection structure sketch of separation household utensils and briquetting, fig. 4 are the utility model provides a overlook structure sketch of first filter plate.

Referring to fig. 1 to 4, the utility model provides an exosome purification device, including: a first filter plate 3, a lower pressing block 5 and a centrifugal turntable 6.

The first filter plate 3 is located inside the filter body 1, the lower end of the first filter plate 3 is clamped with a second filter plate 31, and the end of the second filter plate 31 is clamped with a third filter plate 32.

The lower pressing block 5 is positioned in the separator 2, the lower end of the lower pressing block 5 is attached to the upper surface of the lower fixing ring block 42, and the lower fixing ring block 42 is welded on the outer surface of the separation vessel 4.

And the centrifugal turntable 6 is positioned in the separator 2, and the groove surface of the centrifugal turntable 6 is attached to the lower end surface of the separation vessel 4.

When the exosome is centrifugally purified, a large amount of exosomes are easy to aggregate into blocks by high-speed rotation, and the purification purity is insufficient, so that the purification effect of the exosomes is influenced. Therefore, the utility model discloses an exosome purification device utilizes and filters the inside three layers filter plate membrane of organism, carry out ultrafiltration separation to exosome solution, be convenient for the rapid draing can't pass through the material in space, be convenient for effectual purification, ultrafiltration major part exosomes, be convenient for ensure exosome's integrality and biological activity, rethread low-speed centrifugal motor drives separator ware centrifugation rotatory, the exosome that makes after the inside ultrafiltration of separation household utensils carries out low-speed centrifugal separation, reduce rotation rate, avoid a large amount of exosomes to gather the integrated package, simultaneously a small amount of exosomes carry out effectual separation under certain rotational speed and time, purifying, ultrafiltration, centrifugal separation's dual purification, improve exosome's purification effect, the problem of influence exosome purification effect has been solved.

The upper end surface of the filter body 1 is provided with a liquid inlet 11, the lower end of the filter body 1 is provided with a liquid outlet 13, and the lower end of the filter body 1 is connected with two groups of electric push rods 14.

The exosome solution enters the interior of the filter body 1 from the liquid inlet 11 and then falls into the separation vessel 4 in the separator 2 from the liquid outlet 13. The two groups of electric push rods 14 push the filter body 1 to move upwards, so that the separation vessel 4 in the separator 2 can be conveniently taken out.

The front end surface of the filter body 1 is hinged with a door body 12, and the left side wall and the right side wall of the separator 2 are welded with connecting blocks 22; and the upper surface of the connecting block 22 is fixed with an electric push rod 14.

The door body 12 is opened, the first filter plate 3, the second filter plate 31 and the third filter plate 32 in the filter body 1 are taken out, and the filter membranes on the filter plates are replaced, so that the next purification work of the exosome is facilitated.

A clamping plate 15 is fixed inside the filter body 1; and the inside of the clamping plate 15 is respectively clamped with a first filter plate 3, a second filter plate 31 and a third filter plate 32, and the upper end side wall of the separation vessel 4 is welded with an upper fixed ring block 41.

The first filter plate 3, the second filter plate 31 and the third filter plate 32 are clamped in the filter body 1 in a row, so that the exosome solution is filtered by the first filter plate 3, the second filter plate 31 and the third filter plate 32 respectively. The inner part of the upper fixing ring block 41 is connected with an adjusting stud 5 through threads.

The middle parts of the first filter plate 3, the second filter plate 31 and the third filter plate 32 are provided with a concave arc filter membrane 33, and the upper surface of the centrifugal turntable 6 is fixed with four groups of plug-in columns 61; and the insertion column 61 is inserted into the lower end of the lower pressing block 5.

Concave arc filter membrane 33 makes things convenient for the downstream of exosome solution, and the filter membrane pore of three-layer shape filter membrane 33 diminishes gradually, makes things convenient for ultrafiltration exosome solution, obtains the exosome solution after the ultrafiltration purification, further purifies the exosome through centrifugal separation again, improves the purification effect of exosome.

The upper end surface of the separator 2 is connected with an upper cover 21 in a pressing way, and the lower end of the centrifugal turntable 6 is connected with a low-speed centrifugal motor 63 through a centrifugal rotating shaft 62; and a low speed centrifugal motor 63 is fixed within the bottom of the separator 2.

The centrifuged exosome can be taken out by opening the upper lid 21. The low-speed centrifugal motor 63 drives the centrifugal turntable 6 to rotate at a low speed through the centrifugal rotating shaft 62, and the ultrafiltered exosome is further separated and purified after a certain time and at a certain rotating speed.

The bearing on the surface of the upper end of the lower pressing block 5 is connected with an adjusting stud 52; and the adjusting stud 52 is penetratingly connected at the upper end of the upper fixed ring block 41, and the lower press block 5 is connected with the upper fixed ring block 41 by a compression spring 51.

The adjusting stud 5 is rotated to drive the lower pressing block 5 to move up and down, so that the lower pressing block 5 and the inserting column 61 are conveniently inserted and separated, and the separation vessel 4 and the centrifugal turntable 6 are conveniently separated and fixed. The compression spring 51 functions to press the lower press piece 5 downward.

The utility model discloses an exosome purification device can effectively solve the problem that influences exosome purification effect, and ultrafiltration, centrifugal separation's dual purification improve exosome's purification effect, are applicable to exosome purification device.

Claims

1. An exosome purification device, comprising:

the filter comprises a first filter plate (3) positioned in a filter body (1), wherein a second filter plate (31) is clamped at the lower end of the first filter plate (3), and a third filter plate (32) is clamped at the end of the second filter plate (31);

the lower pressing block (5) is positioned in the separator (2), the lower end of the lower pressing block (5) is attached to the upper surface of the lower fixing ring block (42), and the lower fixing ring block (42) is welded on the outer surface of the separation vessel (4);

the centrifugal rotating disc (6) is positioned inside the separator (2), and the groove surface of the centrifugal rotating disc (6) is attached to the lower end face of the separation vessel (4).

2. An exosome purification device according to claim 1, wherein a liquid inlet (11) is arranged on the upper end surface of the filtering machine body (1), a liquid outlet (13) is arranged at the lower end of the filtering machine body (1), and two groups of electric push rods (14) are connected to the lower end of the filtering machine body (1).

3. An exosome purification device according to claim 1, characterized in that a door body (12) is hinged on the front end surface of the filter body (1), and the left and right side walls of the separator (2) are welded with joining blocks (22); and the upper surface of the connecting block (22) is fixed with an electric push rod (14).

4. An exosome purification device according to claim 1, characterized in that a snap plate (15) is fixed inside said filtering body (1); and the inside joint of joint board (15) has first filter plate (3), second filter plate (31) and third filter plate (32) respectively, separation household utensils (4) upper end lateral wall welding has last fixed ring piece (41).

5. An exosome purification device according to claim 1, characterized in that a concave arc-shaped filter membrane (33) is arranged in the middle of the first filter plate (3), the second filter plate (31) and the third filter plate (32), and four groups of insertion columns (61) are fixed on the upper surface of the centrifugal turntable (6); and the inserting column (61) is inserted in the lower end of the lower pressing block (5).

6. An exosome purification device according to claim 1, wherein the upper end surface of the separator (2) is press-fit connected with an upper cover (21), and the lower end of the centrifugal turntable (6) is connected with a low-speed centrifugal motor (63) through a centrifugal rotating shaft (62); and the low-speed centrifugal motor (63) is fixed in the bottom of the separator (2).

7. An exosome purification device according to claim 1, wherein an adjusting stud (52) is bearing-connected to the upper end surface of the lower pressing block (5); and the adjusting stud (52) is connected to the upper end of the upper fixing ring block (41) in a penetrating manner, and the lower pressing block (5) is connected with the upper fixing ring block (41) through a compression spring (51).