CN111575165B - Sleeve filtering device for separating and purifying exosomes at normal temperature - Google Patents
Sleeve filtering device for separating and purifying exosomes at normal temperature Download PDFInfo
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- CN111575165B CN111575165B CN202010475054.9A CN202010475054A CN111575165B CN 111575165 B CN111575165 B CN 111575165B CN 202010475054 A CN202010475054 A CN 202010475054A CN 111575165 B CN111575165 B CN 111575165B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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Abstract
The invention discloses a casing filtering device for separating and purifying exosomes at normal temperature, which comprises an outer pipe and an inner pipe arranged in the outer pipe, wherein a liquid outlet is formed in the bottom of the inner pipe, a spiral cover is arranged on the outer pipe, and a power mechanism is arranged on the spiral cover; the power mechanism comprises a micro motor fixed on the spiral cover and a transmission part fixedly connected with the micro motor, wherein a filtering mechanism is arranged on the transmission part and is arranged in the inner pipe. The casing filtering device for separating and purifying exosomes at normal temperature can reduce the adhesion of substances with higher relative molecular mass on the first filter screen and reduce the condition that the filter holes of the first filter screen are blocked, thereby ensuring that the exosomes in liquid are not seriously lost after being filtered by the first filter screen; the liquid can be filtered cleanly through the second filter screen and the circular microporous filter, and the filtered exosome flows into the outer tube, so that the exosome with higher purity is obtained.
Description
Technical Field
The invention relates to the technical field of biological equipment, in particular to a sleeve filtering device for separating and purifying exosomes at normal temperature.
Background
Exosomes are membrane-derived vesicles with a lipid bilayer structure, which have diameters of 30-1000nm and contain various components such as proteins, lipids, nucleic acids and the like, are present in various body fluids and culture cell supernatants and participate in a plurality of important physiological or pathological processes of the human body. The quality of exosomes is affected by many factors when they are obtained, and different extraction methods can affect the content and activity of exosomes and their contents. The ultrafiltration centrifugation method is to selectively separate by utilizing ultrafiltration membranes with different interception relative molecular masses, and the method is simple and efficient, but has the defects that substances with higher relative molecular masses easily block filter holes, the extracted exosomes have more impurity proteins and the exosomes are seriously lost.
The existing exosome sleeve filtering device is generally not provided with a filter screen cleaning structure, such as the patent with publication number of CN207998604U, but circular micropores are formed in the wall and the bottom of the inner tube, and the cleaning structure is not provided, so that the defect that the circular micropores are easy to be blocked exists. Therefore, there is a need for a cannula filter device that can obtain higher purity exosomes without serious exosomes loss.
Disclosure of Invention
The invention aims to provide a casing filter device for separating and purifying exosomes at normal temperature, so as to solve the problems in the prior art.
In order to achieve the above object, the present invention provides the following solutions: the invention provides a casing filtering device for separating and purifying exosomes at normal temperature, which comprises an outer pipe and an inner pipe arranged in the outer pipe, wherein a liquid outlet is formed in the bottom of the inner pipe, a spiral cover is arranged on the outer pipe, and a power mechanism is arranged on the spiral cover; the power mechanism comprises a micro motor fixed on the spiral cover and a transmission part fixedly connected with the micro motor, wherein a filtering mechanism is arranged on the transmission part and is arranged in the inner pipe.
Preferably, the transmission piece comprises a first transmission rod fixedly connected with the micro motor, a second transmission rod detachably connected with the first transmission rod and a third transmission rod slidably connected with the second transmission rod.
Preferably, the bottom end of the first transmission rod is fixedly connected with a first connecting block, the top end of the second transmission rod is fixedly connected with a second connecting block, a chute is formed in the first connecting block, a rotatable connecting key is mounted on the second connecting block, and the connecting key is matched with the chute; the first connecting block is provided with a key hole communicated with the chute, and the key hole is matched with the connecting key.
Preferably, the first connecting block bottom with the equal fixedly connected with arc protruding in second connecting block top, the arc protruding corresponds the setting.
Preferably, the bottom end of the second transmission rod is provided with a connecting groove, the top end of the third transmission rod is fixedly connected with a limiting plate, and the limiting plate is matched with the connecting groove; and a first spring is arranged between the limiting plate and the second transmission rod.
Preferably, one end of the third transmission rod, which is close to the limiting plate, is a screw shaft, a rotary table and a circular ring sleeve are sleeved on the screw shaft, and the circular ring sleeve is positioned below the rotary table; and a second spring is sleeved on the third transmission rod and positioned below the circular ring sleeve.
Preferably, the filtering mechanism comprises a first filter screen fixed on the second transmission rod and a second filter screen fixed at the bottom end of the third transmission rod; the first filter screen is in sliding connection with the inner pipe.
Preferably, circular micropores are formed in the wall of the inner tube between the first filter screen and the second filter screen.
Preferably, the inner wall of the port of the outer tube is fixedly connected with a bump, and the inner tube is hung on the bump.
The invention discloses the following technical effects: according to the casing filtering device for separating and purifying exosomes at normal temperature, the micro motor drives the second transmission rod to rotate by utilizing the first transmission rod, so that the first filter screen on the second transmission rod is driven, the adhesion of substances with higher relative molecular mass on the first filter screen is reduced, the condition that the filter holes of the first filter screen are blocked is reduced, and the exosomes in liquid are prevented from being seriously lost after the first filter screen is filtered; through once filtering the back, impurity in the liquid reduces, when filtering through second filter screen and circular micropore, can reduce second filter screen filtration pore and circular micropore and be blocked the condition, can make liquid filterable cleaner through many times filtration, in the exosome flow into the outer tube of filtration to obtain the exosome of higher purity.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1A;
FIG. 3 is an enlarged view of a portion of B in FIG. 1;
FIG. 4 is a cross-sectional view of a first connector block;
the device comprises an outer tube 1, an inner tube 2, a liquid outlet 3, a rotary cover 4, a miniature motor 5, a first transmission rod 6, a second transmission rod 7, a third transmission rod 8, a first connecting block 9, a second connecting block 10, a sliding chute 11, a connecting key 12, a key hole 13, an arc-shaped bulge 14, a connecting groove 15, a limiting plate 16, a first spring 17, a screw shaft 18, a rotary disc 19, a circular ring sleeve 20, a second spring 21, a first filter screen 22, a second filter screen 23 and a convex block 25.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
As shown in fig. 1-4, the invention provides a casing filtering device for separating and purifying exosomes at normal temperature, which comprises an outer pipe 1 and an inner pipe 2 arranged in the outer pipe 1, wherein a liquid outlet 3 is formed in the bottom of the inner pipe 2, a spiral cover 4 is arranged on the outer pipe 1, and a power mechanism is arranged on the spiral cover 4; the power mechanism comprises a micro motor 5 fixed on the rotary cover 4 and a transmission part fixedly connected with the micro motor 5, wherein the transmission part comprises a first transmission rod 6 fixedly connected with the micro motor 5, a second transmission rod 7 detachably connected with the first transmission rod 6 and a third transmission rod 8 slidably connected with the second transmission rod 7; the transmission part is provided with a filtering mechanism, the filtering mechanism is arranged in the inner pipe 2 and comprises a first filter screen 22 fixed on the second transmission rod 7 and a second filter screen 23 fixed at the bottom end of the third transmission rod 8; the first filter screen 22 is slidably connected with the inner tube 2, and circular micropores are formed in the wall of the inner tube 2 between the first filter screen 22 and the second filter screen 23.
When the liquid containing the exosomes is poured into the inner pipe 2, the spiral cover 4 is covered, the micro motor 5 is started, the micro motor 5 drives the second transmission rod 7 to rotate by utilizing the first transmission rod 6, and then drives the first filter screen 22 on the second transmission rod 7, so that the adhesion of substances with higher relative molecular mass on the first filter screen 22 is reduced, the condition that the filter holes of the first filter screen 22 are blocked is reduced, and the exosomes in the liquid are prevented from being seriously lost after the first filter screen 22 is filtered; after once filtering, impurities in the liquid are reduced, when the liquid is filtered through the second filter screen 23 and the circular micropores, the condition that the filter holes of the second filter screen 23 and the circular micropores are blocked can be reduced, the liquid can be filtered cleanly through multiple times of filtering, and filtered exosomes flow into the outer tube 1, so that the exosomes with higher purity are obtained.
In a further optimization scheme, in order to facilitate the connection between the first transmission rod 6 and the second transmission rod 7, a first connecting block 9 is fixedly connected to the bottom end of the first transmission rod 6, a second connecting block 10 is fixedly connected to the top end of the second transmission rod 7, a sliding groove 11 is formed in the first connecting block 9, a rotatable connecting key 12 is mounted on the second connecting block 10, and the connecting key 12 is matched with the sliding groove 11; the first connecting block 9 is provided with a key hole 13 communicated with the chute 11, and the key hole 13 is matched with the connecting key 12. When in connection, the first transmission rod 6 and the second transmission rod 7 can be connected by only inserting the connecting key 12 into the chute 11 through the key hole 13 and adjusting the position of the connecting key 12, and meanwhile, the connecting key 12 is limited by the chute 11, so that the first connecting block 9 can drive the connecting key 12 to rotate.
Further, the bottom end of the first connecting block 9 and the top end of the second connecting block 10 are fixedly connected with arc-shaped protrusions 14, and the arc-shaped protrusions 14 are correspondingly arranged; a connecting groove 15 is formed in the bottom end of the second transmission rod 7, a limiting plate 16 is fixedly connected to the top end of the third transmission rod 8, and the limiting plate 16 is matched with the connecting groove 15; a first spring 17 is arranged between the limiting plate 16 and the second transmission rod 7. When the first connecting block 9 contacts with the bulge on the second connecting block 10, the second transmission rod 7 moves downwards under the action of the arc bulge 14, and when the second transmission rod 7 moves downwards, the first spring 17 is in a compressed state, and when the positions of the arc bulges 14 on the first connecting block 9 and the second connecting block 10 are staggered, the second transmission rod 7 moves upwards under the action of the first spring 17, so that the first filter screen 22 floats up and down, the adhesion of substances with higher relative molecular mass on the first filter screen 22 is reduced, the condition that the filter holes of the first filter screen 22 are blocked is reduced, and the filtering effect of the first filter screen 22 is improved.
According to a further optimization scheme, the possibility that the second filter screen 23 and the round micropores are blocked in the liquid filtered by the first filter screen 22 is not avoided, one end, close to the limiting plate 16, of the third transmission rod 8 is provided with a screw shaft 18, the screw shaft 18 is sleeved with a rotary disc 19 and a circular ring sleeve 20, the rotary disc 19 is in contact connection with the second transmission rod 7, and the circular ring sleeve 20 is positioned below the rotary disc 19; the third transmission rod 8 is sleeved with a second spring 21, the second spring 21 is located below the circular ring sleeve 20, and the circular ring sleeve 20 always keeps close contact with the turntable 19 under the action of the second spring 21. When the second transmission rod 7 moves downwards, the rotary disc 19 sleeved on the screw shaft 18 moves downwards under the pushing of the second transmission rod 7 and rotates on the screw rod, and when the second transmission rod 7 moves upwards, the annular sleeve 20 pushes the rotary disc 19 to rotate and simultaneously moves upwards under the action of the second spring 21, so that liquid between the first filter screen 22 and the second filter screen 23 is stirred by the rotary disc 19, substances with relatively high molecular mass possibly existing in the liquid cannot be attached to the filter screen, and the situation that filter holes and round micropores are blocked is avoided.
In a further optimization scheme, in order to facilitate the installation of the inner tube 2 and the outer tube 1, a bump 25 is fixedly connected to the inner wall of the port of the outer tube 1, and the inner tube 2 is suspended on the bump 25. In order to provide a sealing effect between the inner tube 2 and the screw cap 4, a sealing ring (not shown) is provided at the top of the port of the inner tube 2, so as to avoid mixing of unfiltered liquid into the outer tube 1.
In the description of the present invention, it should be understood that the terms "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (3)
1. The casing filtering device for separating and purifying exosomes at normal temperature is characterized by comprising an outer pipe (1) and an inner pipe (2) arranged in the outer pipe (1), wherein a liquid outlet (3) is formed in the bottom of the inner pipe (2), a spiral cover (4) is arranged on the outer pipe (1), and a power mechanism is arranged on the spiral cover (4); the power mechanism comprises a micro motor (5) fixed on the spiral cover (4) and a transmission part fixedly connected with the micro motor (5), a filtering mechanism is arranged on the transmission part, and the filtering mechanism is arranged in the inner pipe (2);
the transmission part comprises a first transmission rod (6) fixedly connected with the miniature motor (5), a second transmission rod (7) detachably connected with the first transmission rod (6) and a third transmission rod (8) slidably connected with the second transmission rod (7);
the novel transmission device is characterized in that a first connecting block (9) is fixedly connected to the bottom end of the first transmission rod (6), a second connecting block (10) is fixedly connected to the top end of the second transmission rod (7), a sliding groove (11) is formed in the first connecting block (9), a rotatable connecting key (12) is mounted on the second connecting block (10), and the connecting key (12) is matched with the sliding groove (11); a key hole (13) communicated with the chute (11) is formed in the first connecting block (9), and the key hole (13) is matched with the connecting key (12);
the bottom end of the first connecting block (9) and the top end of the second connecting block (10) are fixedly connected with arc-shaped bulges (14), and the arc-shaped bulges (14) are correspondingly arranged;
a connecting groove (15) is formed in the bottom end of the second transmission rod (7), a limiting plate (16) is fixedly connected to the top end of the third transmission rod (8), and the limiting plate (16) is matched with the connecting groove (15); a first spring (17) is arranged between the limiting plate (16) and the second transmission rod (7);
one end, close to the limiting plate (16), of the third transmission rod (8) is a screw shaft (18), a rotary disc (19) and a circular ring sleeve (20) are sleeved on the screw shaft (18), the rotary disc (19) is in contact connection with the second transmission rod (7), and the circular ring sleeve (20) is positioned below the rotary disc (19); a second spring (21) is sleeved on the third transmission rod (8), and the second spring (21) is positioned below the circular sleeve (20);
the filtering mechanism comprises a first filter screen (22) fixed on the second transmission rod (7) and a second filter screen (23) fixed at the bottom end of the third transmission rod (8); the first filter screen (22) is in sliding connection with the inner tube (2).
2. The normal temperature separation and purification exosome sleeve filtration device according to claim 1, wherein: circular micropores are formed in the pipe wall of the inner pipe (2) between the first filter screen (22) and the second filter screen (23).
3. The normal temperature separation and purification exosome sleeve filtration device according to claim 1, wherein: the inner wall of the port of the outer tube (1) is fixedly connected with a bump (25), and the inner tube (2) is hung on the bump (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010475054.9A CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010475054.9A CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| Publication Number | Publication Date |
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| CN111575165A CN111575165A (en) | 2020-08-25 |
| CN111575165B true CN111575165B (en) | 2023-10-03 |
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| CN202010475054.9A Active CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| CN112662532B (en) * | 2021-01-11 | 2023-03-14 | 新乡医学院三全学院 | Stem cell source exosome rotary separation device |
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