CN111575165A - Sleeve filtering device for separating and purifying exosome at normal temperature - Google Patents
Sleeve filtering device for separating and purifying exosome at normal temperature Download PDFInfo
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- CN111575165A CN111575165A CN202010475054.9A CN202010475054A CN111575165A CN 111575165 A CN111575165 A CN 111575165A CN 202010475054 A CN202010475054 A CN 202010475054A CN 111575165 A CN111575165 A CN 111575165A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a sleeve filtering device for separating and purifying exosomes at normal temperature, which comprises an outer pipe and an inner pipe arranged in the outer pipe, wherein the bottom of the inner pipe is provided with a liquid outlet, the outer pipe is provided with a screw cap, and the screw cap is provided with a power mechanism; the power mechanism comprises a micro motor fixed on the screw cap and a transmission part fixedly connected with the micro motor, a filtering mechanism is mounted on the transmission part, and the filtering mechanism is arranged in the inner pipe. The casing filtering device for separating and purifying exosomes at normal temperature can reduce the adhesion of substances with higher relative molecular mass on the first filter screen and reduce the blocking of the filter holes of the first filter screen, thereby ensuring that exosomes in liquid cannot be seriously lost after being filtered by the first filter screen; through second filter screen and circular micropore filtration, can make liquid filterable cleaner through filtering many times, the exosome that filters flows into in the outer tube to obtain the exosome of higher purity.
Description
Technical Field
The invention relates to the technical field of biological equipment, in particular to a sleeve filtering device for separating and purifying exosomes at normal temperature.
Background
Exosomes are membrane-derived vesicles with a diameter of 30-1000nm and containing various components such as proteins, lipids and nucleic acids, and have a lipid bilayer structure, exist in various body fluids and culture cell supernatants, and participate in many important physiological or pathological processes of a human body. The quality of exosomes is influenced by a plurality of factors when exosomes are obtained, and different extraction methods influence the content and activity of exosomes and the content of exosomes. The ultrafiltration centrifugation method is to use ultrafiltration membranes with different intercepted relative molecular masses for selective separation, and the method is simple and efficient, but has the defects that substances with higher relative molecular masses easily block filter holes, and the extracted exosomes have more impurity proteins and are seriously lost.
The existing exosome casing filtering device is not provided with a filter screen cleaning structure generally, for example, a patent with the publication number of CN207998604U, only circular micropores are arranged on the wall and the bottom of an inner tube, but the cleaning structure is not arranged, so that the defect that the circular micropores are easily blocked exists. Therefore, there is a need for a cannula filtration device that can obtain high purity exosomes without causing significant exosome loss.
Disclosure of Invention
The object of the present invention is to provide a solution to the above-mentioned problems of the prior art, and to provide a solution to the above problems
In order to achieve the purpose, the invention provides the following scheme: the invention provides a sleeve filtering device for separating and purifying exosomes at normal temperature, which comprises an outer pipe and an inner pipe arranged in the outer pipe, wherein the bottom of the inner pipe is provided with a liquid outlet, the outer pipe is provided with a screw cap, and the screw cap is provided with a power mechanism; the power mechanism comprises a micro motor fixed on the screw cap and a transmission part fixedly connected with the micro motor, a filtering mechanism is mounted on the transmission part, and the filtering mechanism is arranged in the inner pipe.
Preferably, the transmission part comprises a first transmission rod fixedly connected with the micro motor, a second transmission rod detachably connected with the first transmission rod, and a third transmission rod slidably connected with the second transmission rod.
Preferably, the bottom end of the first transmission rod is fixedly connected with a first connecting block, the top end of the second transmission rod is fixedly connected with a second connecting block, a sliding groove is formed in the first connecting block, a rotatable connecting key is mounted on the second connecting block, and the connecting key is matched with the sliding groove; the first connecting block is provided with a key hole communicated with the sliding groove, and the key hole is matched with the connecting key.
Preferably, the bottom end of the first connecting block and the top end of the second connecting block are fixedly connected with arc bulges, and the arc bulges are correspondingly arranged.
Preferably, the bottom end of the second transmission rod is provided with a connection groove, the top end of the third transmission rod is fixedly connected with a limiting plate, and the limiting plate is matched with the connection groove; and a first spring is arranged between the limiting plate and the second transmission rod.
Preferably, one end of the third transmission rod, which is close to the limiting plate, is a spiral shaft, a turntable and a circular ring sleeve are sleeved on the spiral shaft, and the circular ring sleeve is positioned below the turntable; and a second spring is sleeved on the third transmission rod and is positioned below the circular ring sleeve.
Preferably, the filtering mechanism comprises a first filter screen fixed on the second transmission rod and a second filter screen fixed at the bottom end of the third transmission rod; the first filter screen is connected with the inner pipe in a sliding mode.
Preferably, the inner pipe wall between the first filter screen and the second filter screen is provided with round micropores.
Preferably, a bump is fixedly connected to the inner wall of the port of the outer tube, and the inner tube is hung on the bump.
The invention discloses the following technical effects: according to the casing filtering device for separating and purifying exosomes at normal temperature, the micro motor drives the second transmission rod to rotate by using the first transmission rod, so that the first filter screen on the second transmission rod is driven, the adhesion of substances with higher relative molecular mass on the first filter screen is reduced, the blocking condition of the filter holes of the first filter screen is reduced, and the exosomes in liquid are prevented from being seriously lost after being filtered by the first filter screen; after once filtering, impurity in the liquid reduces, when filtering through second filter screen and circular micropore, can reduce the condition that second filter screen filtration pore and circular micropore are blocked, can make liquid filterable cleaner through filtration many times, and the exosome that filters flows into in the outer tube to obtain the exosome of higher purity.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of a portion of FIG. 1;
FIG. 3 is an enlarged view of a portion B of FIG. 1;
FIG. 4 is a cross-sectional view of a first connecting block;
wherein, 1 outer tube, 2 inner tubes, 3 liquid outlets, 4 spiral covers, 5 micro motor, 6 first transfer lines, 7 second transfer lines, 8 third transfer lines, 9 first connecting blocks, 10 second connecting blocks, 11 chutes, 12 connecting keys, 13 keyholes, 14 arc-shaped protrusions, 15 connecting grooves, 16 limiting plates, 17 first springs, 18 spiral shafts, 19 turntables, 20 circular ring sleeves, 21 second springs, 22 first filter screens, 23 second filter screens, 24 circular micropores, 25 lugs.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
As shown in fig. 1-4, the present invention provides a casing filtering device for separating and purifying exosomes at normal temperature, comprising an outer tube 1 and an inner tube 2 arranged in the outer tube 1, wherein the bottom of the inner tube 2 is provided with a liquid outlet 3, the outer tube 1 is provided with a screw cap 4, and the screw cap 4 is provided with a power mechanism; the power mechanism comprises a micro motor 5 fixed on the rotary cover 4 and a transmission part fixedly connected with the micro motor 5, and the transmission part comprises a first transmission rod 6 fixedly connected with the micro motor 5, a second transmission rod 7 detachably connected with the first transmission rod 6 and a third transmission rod 8 in sliding connection with the second transmission rod 7; the transmission part is provided with a filtering mechanism which is arranged in the inner pipe 2, and the filtering mechanism comprises a first filter screen 22 fixed on the second transmission rod 7 and a second filter screen 23 fixed at the bottom end of the third transmission rod 8; the first filter screen 22 is connected with the inner tube 2 in a sliding manner, and round micropores 24 are formed in the tube wall of the inner tube 2 between the first filter screen 22 and the second filter screen 23.
When the device is used, liquid containing exosomes is poured into the inner tube 2, the screw cap 4 is covered, the micro motor 5 is started, the micro motor 5 drives the second transmission rod 7 to rotate by utilizing the first transmission rod 6, and then the first filter screen 22 on the second transmission rod 7 is driven, so that the adhesion of substances with relatively high molecular mass to the first filter screen 22 is reduced, the blocking situation of the filter holes of the first filter screen 22 is reduced, and the exosomes in the liquid are prevented from being seriously lost after being filtered by the first filter screen 22; after once filtering, impurity in the liquid reduces, when filtering through second filter screen 23 and circular micropore 24, can reduce the circumstances that second filter screen 23 filtration pore and circular micropore 24 were blocked, can make liquid filterable cleaner through filtering many times, and the exosome that filters flows into in outer tube 1 to obtain the exosome of higher purity.
In order to facilitate the connection between the first transmission rod 6 and the second transmission rod 7, a first connecting block 9 is fixedly connected to the bottom end of the first transmission rod 6, a second connecting block 10 is fixedly connected to the top end of the second transmission rod 7, a sliding groove 11 is formed in the first connecting block 9, a rotatable connecting key 12 is mounted on the second connecting block 10, and the connecting key 12 is matched with the sliding groove 11; the first connecting block 9 is provided with a key hole 13 communicated with the sliding groove 11, and the key hole 13 is matched with the connecting key 12. When the connection, only need insert the connecting key 12 into the spout 11 by keyhole 13, adjust the position of connecting key 12, can realize being connected of first transfer line 6 and second transfer line 7, utilize spout 11 to carry on spacingly to connecting key 12 simultaneously to make first link 9 can drive connecting key 12 rotatory.
Further, arc-shaped protrusions 14 are fixedly connected to the bottom end of the first connecting block 9 and the top end of the second connecting block 10, and the arc-shaped protrusions 14 are arranged correspondingly; the bottom end of the second transmission rod 7 is provided with a connecting groove 15, the top end of the third transmission rod 8 is fixedly connected with a limiting plate 16, and the limiting plate 16 is matched with the connecting groove 15; a first spring 17 is arranged between the limit plate 16 and the second transmission rod 7. When the first connecting block 9 contacts with the protrusion on the second connecting block 10, the second transmission rod 7 is moved downwards under the action of the arc-shaped protrusion 14, when the second transmission rod 7 moves downwards, the first spring 17 is in a compressed state, when the positions of the arc-shaped protrusions 14 on the first connecting block 9 and the second connecting block 10 are staggered, the second transmission rod 7 moves upwards under the action of the first spring 17, so that the first filter screen 22 floats up and down, the adhesion of substances with higher relative molecular mass on the first filter screen 22 is reduced, the blocking condition of the filter holes of the first filter screen 22 is reduced, and the filtering effect of the first filter screen 22 is improved.
According to the further optimization scheme, the possibility that the liquid filtered by the first filter screen 22 blocks the second filter screen 23 and the round micropores 24 is not avoided, the end, close to the limiting plate 16, of the third transmission rod 8 is a spiral shaft 18, a rotary disc 19 and a circular ring sleeve 20 are sleeved on the spiral shaft 18, the rotary disc 19 is in contact connection with the second transmission rod 7, and the circular ring sleeve 20 is located below the rotary disc 19; and a second spring 21 is sleeved on the third transmission rod 8, the second spring 21 is positioned below the circular sleeve 20, and the circular sleeve 20 is always in close contact with the turntable 19 under the action of the second spring 21. When the second transmission rod 7 moves downwards, the rotating disc 19 sleeved on the spiral shaft 18 moves downwards under the pushing of the second transmission rod 7 and rotates on the spiral rod, and when the second transmission rod 7 moves upwards, the circular ring sleeve 20 pushes the rotating disc 19 to rotate and move upwards under the action of the second spring 21, so that the liquid between the first filter screen 22 and the second filter screen 23 is stirred by the rotating disc 19, substances possibly existing in the liquid and having high relative molecular mass cannot be attached to the filter screens, and the situation that the filter holes and the circular micropores 24 are blocked is avoided.
According to a further optimized scheme, in order to facilitate the installation of the inner tube 2 and the outer tube 1, a convex block 25 is fixedly connected to the inner wall of the port of the outer tube 1, and the inner tube 2 is hung on the convex block 25. In order to achieve the sealing effect between the inner tube 2 and the screw cap 4, a sealing ring (not shown) is disposed at the top of the port of the inner tube 2, so as to prevent unfiltered liquid from being mixed into the outer tube 1.
In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (9)
1. The casing filtering device for separating and purifying exosome at normal temperature is characterized by comprising an outer pipe (1) and an inner pipe (2) arranged in the outer pipe (1), wherein a liquid outlet (3) is formed in the bottom of the inner pipe (2), a screw cap (4) is installed on the outer pipe (1), and a power mechanism is installed on the screw cap (4); the power mechanism comprises a micro motor (5) fixed on the rotary cover (4) and a transmission part fixedly connected with the micro motor (5), and a filtering mechanism is installed on the transmission part and arranged in the inner pipe (2).
2. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 1, which is characterized in that: the transmission part comprises a first transmission rod (6) fixedly connected with the micro motor (5), a second transmission rod (7) detachably connected with the first transmission rod (6) and a third transmission rod (8) slidably connected with the second transmission rod (7).
3. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 2, characterized in that: the bottom end of the first transmission rod (6) is fixedly connected with a first connecting block (9), the top end of the second transmission rod (7) is fixedly connected with a second connecting block (10), a sliding groove (11) is formed in the first connecting block (9), a rotatable connecting key (12) is mounted on the second connecting block (10), and the connecting key (12) is matched with the sliding groove (11); the first connecting block (9) is provided with a key hole (13) communicated with the sliding groove (11), and the key hole (13) is matched with the connecting key (12).
4. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 3, characterized in that: the bottom end of the first connecting block (9) and the top end of the second connecting block (10) are fixedly connected with arc-shaped bulges (14), and the arc-shaped bulges (14) are correspondingly arranged.
5. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 4, characterized in that: a connecting groove (15) is formed in the bottom end of the second transmission rod (7), a limiting plate (16) is fixedly connected to the top end of the third transmission rod (8), and the limiting plate (16) is matched with the connecting groove (15); and a first spring (17) is arranged between the limiting plate (16) and the second transmission rod (7).
6. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 5, characterized in that: a screw shaft (18) is arranged at one end, close to the limiting plate (16), of the third transmission rod (8), a rotary disc (19) and a circular ring sleeve (20) are sleeved on the screw shaft (18), the rotary disc (19) is in contact connection with the second transmission rod (7), and the circular ring sleeve (20) is located below the rotary disc (19); and a second spring (21) is sleeved on the third transmission rod (8), and the second spring (21) is positioned below the circular ring sleeve (20).
7. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 2, characterized in that: the filtering mechanism comprises a first filter screen (22) fixed on the second transmission rod (7) and a second filter screen (23) fixed at the bottom end of the third transmission rod (8); the first filter screen (22) is connected with the inner pipe (2) in a sliding mode.
8. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 7, characterized in that: round micropores (24) are formed in the pipe wall of the inner pipe (2) between the first filter screen (22) and the second filter screen (23).
9. The normal-temperature casing filtering device for separating and purifying exosomes according to claim 1, which is characterized in that: the inner wall of the port of the outer tube (1) is fixedly connected with a lug (25), and the inner tube (2) is hung on the lug (25).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010475054.9A CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010475054.9A CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| CN111575165A true CN111575165A (en) | 2020-08-25 |
| CN111575165B CN111575165B (en) | 2023-10-03 |
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| CN202010475054.9A Active CN111575165B (en) | 2020-05-29 | 2020-05-29 | Sleeve filtering device for separating and purifying exosomes at normal temperature |
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| CN112662532A (en) * | 2021-01-11 | 2021-04-16 | 新乡医学院三全学院 | Stem cell source exosome rotary separation device |
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| CN111575165B (en) | 2023-10-03 |
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