CN112662532A - Stem cell source exosome rotary separation device - Google Patents

Abstract

The invention relates to the technical field of medicines, in particular to a stem cell source exosome rotary separation device which comprises a base and a centrifugal seat, wherein a centrifugal motor is fixed in the base, a plurality of centrifugal jacks are formed in the centrifugal seat, the centrifugal motor is connected with a driving structure for driving a centrifugal tube to rotate, a supporting tube extending into the centrifugal tube is fixed on the centrifugal seat, a blocking structure for releasing or blocking liquid flowing upwards in the centrifugal tube is arranged on the supporting tube, the blocking structure is connected with a retraction and release unit, a liquid pumping tube and a return tube are fixed in the supporting tube, the liquid pumping tube and the return tube are respectively communicated with a left flow distribution and collection valve and a right flow distribution and collection valve, the left flow distribution and collection valve is communicated with a filtering unit, the filtering unit is communicated with a micro-circulation water pump, and the micro-circulation water pump is communicated with the right flow distribution and collection valve, so that exosome can be quickly separated, improves the separation efficiency and has wide application prospect in the technical field of medicine.

Description

Stem cell source exosome rotary separation device

Technical Field

The invention relates to the technical field of medicines, in particular to a stem cell source exosome rotary separation device.

Background

Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins, and nowadays refer to disc-shaped vesicles with diameters of 40-100nm, which are mainly derived from multivesicular bodies formed by invagination of intracellular lysosomal microparticles, and are released into extracellular matrix after fusion of the multivesicular body outer membrane with cell membrane. Due to the special structure and function of the exosome, the exosome has potential application value, can be used as a biological index for diagnosing various diseases on one hand, can also be used as a treatment means on the other hand, and can be possibly used as a natural carrier of a medicament for clinical treatment in the future. The separation and purification of exosomes are always the concerns of researchers, and the acquisition of high-purity exosomes is of great importance to the subsequent research. At present, people mostly adopt methods such as ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or kits to realize the extraction and separation of exosomes.

The phenomenon described paradoxically in tea is that the tea leaves, when stirred in a cup, tend to wander back to the centre of the cup bottom rather than being urged to the edge of the cup bottom under the action of a helical centrifugal force, as would be expected. The initial explanation came from einstein 1926 a paper explaining the problem of bank erosion (bayer's law). This phenomenon has been used in a new technique for separating red blood cells from plasma and for understanding atmospheric pressure systems.

In the extraction of exosomes, the ultracentrifugation method is the most common method at present, researchers adopting the ultracentrifugation method account for 56% of all exosome separation methods, and the method is widely applied to the analysis of various biological samples, such as serum, plasma, cell culture fluid, urine, saliva, cerebrospinal fluid and the like, and is also the 'gold standard' in the current separation method. However, the separation and extraction method is time-consuming, labor-consuming, highly dependent on manpower, and low in recovery rate, and is inconvenient for people to use.

Disclosure of Invention

In order to solve the problems that the exosome separation and extraction method in the prior art is time-consuming and labor-consuming, highly dependent on manpower, low in recovery rate and inconvenient for people to use, the stem cell source exosome rotary separation device is invented.

The technical scheme of the invention is that the centrifugal centrifuge comprises a base and a centrifugal seat, wherein a centrifugal motor is fixed in the base, an output shaft of the centrifugal motor is fixedly connected with a master gear, the master gear is rotatably connected with a plurality of slave gears, the centrifugal seat is provided with a plurality of centrifugal jacks which are uniformly inserted with centrifuge tubes in an annular array and take the output shaft of the centrifugal motor as the center, the centrifugal jacks are rotatably connected with a plurality of driving rollers through connecting shafts, the connecting shaft connected with one driving roller is fixedly and detachably connected with the corresponding slave gear below, the driving rollers are externally wrapped with a circular table-shaped outer pad, one end of each centrifuge tube in each centrifugal jack is inserted between the driving rollers and tightly abutted against the outer pad, the centrifugal seat is fixed with a supporting plate through a telescopic rod, a supporting tube is fixed on the supporting plate, and one end of the supporting tube penetrates through a test tube plug and extends into the centrifuge tube, the outer side wall of one part of the supporting tube in the centrifugal tube is fixed with a fixing ring, the supporting tube further comprises a sliding ring which is positioned above the fixing ring and is connected with the outer side wall of the supporting tube in a sliding manner, a structure which enables the fixing ring to be far away from the sliding ring is arranged between the sliding ring and the fixing ring, a plurality of corresponding lower supporting rods and upper supporting rods which are in an annular uniform array are hinged on the outer peripheral surface or the upper and lower edges of the fixing ring and the sliding ring, the lower supporting rods are hinged with the upper supporting rods, the supporting tube further comprises an inner filtering membrane which is positioned on the surface of a circular ring formed by each upper supporting rod and is fixed, when the sliding ring slides along the supporting tube, each upper supporting rod rotates around a hinged point between the upper supporting rods and the fixing ring under the action of each lower supporting rod, the inner filtering membrane is contracted or opened relative to the supporting tube, and the structure which is contracted or opened, when the upward flowing liquid is cut off, the inner filtering membrane collects cells with larger diameter or other impurities in the liquid to the central part below the inner filtering membrane in the centrifugal tube, the slip ring is connected with a retraction unit which controls the slip ring to slide along the supporting pipe outside the centrifugal pipe through a plurality of steel wire lines, a liquid pumping pipe and a return pipe are fixed in the supporting pipe, the end part of the return pipe is lower than the end part of the liquid pumping pipe, the supporting pipe of the end part of the water pumping pipe in the centrifugal pipe is provided with a plurality of liquid inlets, the liquid pumping pipe and the return pipe are respectively communicated with a left flow distributing and collecting valve and a right flow distributing and collecting valve, the left flow distributing and collecting valve is communicated with a filtering unit for removing cells with larger diameter or other impurities in the liquid through a communicating pipe, the filter unit is communicated with the micro-circulation water pump through a first liquid conveying pipe, and the micro-circulation water pump is communicated with the right flow distributing and collecting valve through a second liquid conveying pipe.

Preferably, a supporting shaft is fixed at the center of the end face of the slave gear, a first bearing with a seat is fixed on the base, one end, far away from the slave gear, of the supporting shaft is connected to the first bearing with a seat in an interference mode, a connector is fixed at the axis of the other end face of the slave gear, a non-circular inserting hole is formed in the connector, one connecting shaft connected to the driving roller penetrates through the centrifugal seat and extends to the position above the slave gear, a connecting plug matched with the inserting hole is formed in the end portion of the connecting shaft in an extending mode, and the connecting plug is inserted into the inserting hole.

Preferably, the rotation groove has been seted up in the centrifugation jack, the upper and lower inner end face in rotation groove is fixed with second area seat bearing and third area seat bearing respectively, the both ends of connecting axle interference connection respectively are on second area seat bearing and third area seat bearing, the surface of outer pad is rough surface, the centrifugation jack that rotates the groove top is radius platform type, the opening part inside wall of centrifugation jack is fixed with the supporting pad, centrifuging tube and supporting pad are inconsistent.

Preferably, the end of the liquid suction pipe in the centrifugal pipe is positioned in a structure that the internal filtering membrane is contracted or expanded, the end of the return pipe in the centrifugal pipe is positioned on the same horizontal plane with the end of the supporting pipe in the centrifugal pipe, and the filtering hole of the internal filtering membrane has a diameter of 100 nm.

Preferably, a hole inlet line is formed in the supporting tube above the sliding ring, the steel wire extends into the supporting tube through the hole inlet line, a hole outlet line is formed at the joint of the supporting tube and the supporting plate, and one end, far away from the sliding block, of the steel wire extends out of the supporting tube through the hole outlet line.

Preferably, the unit of receive and releasing includes line wheel, locating wheel, axis of rotation, dwang and pin rod, it receive and releases the groove to have seted up in the backup pad, line wheel and locating wheel rotate through the axis of rotation and connect in receiving and releasing the inslot, the line hole has been seted up in the backup pad, the steel wire line extends to in the line hole and with line wheel fixed connection from the stay tube, the tip that the axis of rotation is close to the one end of locating wheel has seted up flexible hole along the length direction of axis of rotation, dwang swing joint is in flexible downthehole, non-circular shape rotation slot has been seted up to the interior bottom in flexible hole, the dwang one end in flexible hole be fixed with the rotation plug that rotates slot looks adaptation, rotate on the dwang and be connected with the connection lantern ring, the slide opening has been seted up in the axis.

Preferably, be fixed with the spacing arch of first spacing arch and second on the dwang of the both sides of the connection lantern ring, the pin rod is the T type, and the pin rod is greater than the diameter of locating wheel with the length of the one section of being connected of the connection lantern ring, the both ends that the pin rod kept away from the connection lantern ring are fixed with first bayonet joint head and second bayonet joint head respectively, receive and release the groove and be close to on the lateral wall of locating wheel set up a plurality of use axis of rotation as the center be the even display of annular with the ratchet matched with bayonet joint groove of locating wheel, work as the rotation plug is kept away from when rotating the slot and is constituted first bayonet joint head and is connected with the ratchet joint of locating wheel, the structure that second bayonet joint groove joint is connected, the one end that the line wheel was kept away from to the dwang runs through the backup pad and is fixed with the runner, the opening part that receives and.

Preferably, the test tube stopper comprises rubber buffer and connecting block fixed connection, the axle center of rubber buffer and connecting block is seted up respectively and is linked together down the hole and go up the rotation hole, the resettlement groove has been seted up to the lateral wall in last rotation hole, the resettlement inslot is provided with the spacing lantern ring of self-lubricating material, the stay tube runs through the spacing lantern ring, the opening part in resettlement groove is fixed with the spacing closing cap of spacing, the lateral wall of the stay tube of spacing lantern ring top is fixed with the spacing arch of third.

Preferably, the filter unit includes filter cartridge, filtration basket, outer filtration membrane and filters the closing cap, the inner wall of filter cartridge is fixed with the supporting shoe, filtration basket joint is on the supporting shoe, outer filtration membrane fixes on the interior terminal surface of filtration basket, outer filtration membrane's filtration pore diameter is 100nm, communicating pipe is linked together with the filter cartridge who filters the basket top, first transfer line is linked together with the lateral wall lower extreme of filter cartridge, filter the opening part at the filter cartridge to be fixed to the closing cap.

The technical scheme of the invention can achieve the following beneficial effects: (1) through the matching of the fixing ring, the sliding ring, the lower support rod, the upper support rod and the inner filtering membrane, the paradox phenomenon of tea leaves is utilized, cells with larger diameters or other impurities in liquid in the centrifugal tube can be conveniently gathered to the center of the centrifugal tube below the inner filtering membrane, and then the cells and other impurities can be conveniently extracted and discharged to obtain required exosomes; (2) through the matching use of the liquid pumping pipe, the backflow pipe, the left flow distributing and collecting valve, the right flow distributing and collecting valve, the first liquid conveying pipe, the second liquid conveying pipe, the micro-circulation water pump and the filtering unit, cells or other impurities with larger diameters, which are intercepted and gathered by an inner filtering membrane in the centrifugal pipe, can be conveniently pumped out and filtered and removed, and meanwhile, the backflow pipe is used for returning filtered liquid into the centrifugal pipe to realize recycling, so that the time and labor are saved, the process of relying on manual operation in a large quantity is avoided, the recovery rate is high, and the use is convenient; (3) through the matching of the centrifugal motor, the main gear, the plurality of secondary gears, the connectors, the inserting holes, the inserting connectors and the plurality of centrifugal inserting holes, the centrifugal motor is convenient to improve, the centrifugal motor drives a plurality of centrifugal tubes to carry out centrifugal filtration to obtain exosomes at the same time, and the working efficiency is improved; (4) through the matching of the driving rollers, the outer pads and the supporting pads, the centrifugal tube is convenient to limit, so that the centrifugal tube is kept to be vertically erected in the centrifugal jack; (5) the telescopic rod and the supporting plate are convenient for taking and placing the supporting tube and the centrifugal tube, and the position of the end part of the supporting rod in the centrifugal tube is also convenient to adjust in the centrifugal tube, so that cells or other impurities with larger diameters, which are intercepted and gathered by the filtering membrane in the centrifugal tube, can be fully extracted; (6) the test tube plug can conveniently rotate relative to the support tube through the limiting sleeve ring, the limiting sealing cover and the third limiting bulge, the friction force between the test tube plug and the support tube is reduced by the limiting sleeve ring made of a self-lubricating material, the support tube is limited by the third limiting bulge, and the damage caused by the fact that the end part of the support tube located in the centrifugal tube is abutted against the bottom end of the centrifugal tube is avoided; the technical scheme of the invention has wide application prospect in the technical field of medicines.

Drawings

FIG. 1 is a front view of a stem cell-derived exosome rotating separation device of the present invention.

Fig. 2 is an enlarged view of the area a in fig. 1.

Fig. 3 is an enlarged view of the region B in fig. 1.

Fig. 4 is an enlarged view of the region C in fig. 1.

Fig. 5 is an enlarged view of the region D in fig. 1.

Fig. 6 is an enlarged view of region E in fig. 1.

Fig. 7 is an enlarged view of region F in fig. 1.

Fig. 8 is an enlarged view of region G in fig. 1.

FIG. 9 is a cross-sectional view of the retraction unit of the present invention.

Wherein, 1, a base, 2, a centrifugal seat, 3, a containing groove, 4, a centrifugal motor, 5, a main gear, 6, a driven gear, 7, a supporting shaft, 8, a first bearing with a seat, 9, a connector, 10, a splicing hole, 11, a splicing head, 12, a connecting shaft, 13, a centrifugal jack, 14, a rotating groove, 15, a driving roller, 16, an outer pad, 17, a third bearing with a seat, 18, a second bearing with a seat, 19, a centrifuge tube, 20, a supporting pad, 21, a rubber plug, 22, a connecting block, 23, a lower hole, 24, an upper rotating hole, 25, a placing groove, 26, a supporting tube, 27, a limiting lantern ring, 28, a third limiting bulge, 29, a limiting sealing cover, 30, a liquid pumping tube, 31, a backflow tube, 32, a liquid inlet, 33, a left shunt valve, a current collecting tube, 34, a communicating tube, 35, a first liquid conveying tube, 36, a micro-cycle water pump, 37, a second liquid conveying tube, 38 and a, 39. the filter comprises a hole inlet wire, 40, a steel wire, 41, a wire outlet hole, 42, a fixing groove, 43, a telescopic rod, 44, a supporting plate, 45, a wire routing hole, 101, a fixing ring, 102, a lower supporting rod, 103, an upper supporting rod, 104, a sliding ring, 105, a connecting spring, 106, an inner filtering membrane, 201, a filter box, 202, a supporting block, 203, a filter basket, 204, an outer filtering membrane, 205, a filter sealing cover, 301, a receiving and releasing groove, 302, a rotating shaft, 303, a wire wheel, 304, a positioning wheel, 305, a telescopic hole, 306, a rotating slot, 307, a rotating rod, 308, a rotating plug, 309, a first limiting protrusion, 310, a second limiting protrusion, 311, a connecting lantern ring, 312, a sliding hole, 313, a pin rod, 314, a first clamping pin head, 315, a first clamping pin head, 316, a clamping pin groove, 317, a rotating wheel, 318 and a receiving and.

Detailed Description

The technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without any creative effort belong to the protection scope of the present invention, and in the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, only for the convenience of describing the present invention and simplifying the description, but not for indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance in the description of the present invention, it being noted that unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as being fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Stem cell source exosome rotary separation device as shown in fig. 1-9, including can dismantle the base 1 and the centrifugal seat 2 of connection through the bolt fastening, be convenient for increase the stability of whole device through base 1 for whole device can be stable stand subaerial, utilizes centrifugal seat 2 to be convenient for lay centrifuging tube 19 simultaneously, makes centrifuging tube 19 can be stable carry out centrifugal operation. Holding tank 3 has been seted up on base 1, can dismantle through the bolt fastening in the holding tank 3 and be connected with centrifuge motor 4, and centrifuge motor 4 is common driving motor on the market, is convenient for carry out centrifugal operation through centrifuge motor 4 for centrifuging tube 19 and provides power. The output shaft of centrifugal motor 4 passes through bolt fastening can be dismantled and is connected with master gear 5, and master gear 5 rotates and is connected with a plurality of gears 6 from, is convenient for drive master gear 5 through centrifugal motor 4 and rotates for master gear 5 drives a plurality of and rotates from gear 6 simultaneously, and then can carry out centrifugal operation to many centrifuging tubes 19 simultaneously. Set up a plurality of centrifugal jacks 13 that use the output shaft of centrifugal motor 4 as the even array of grafting centrifuging tube 19 of annular of being of center on the centrifugal seat 2, be convenient for place centrifuging tube 19 through a plurality of centrifugal jacks 13, realize carrying out centrifugal operation to a plurality of centrifuging tubes 19 simultaneously. It is connected with a plurality of drive rollers 15 to rotate through connecting axle 12 in the centrifugation jack 13, connecting axle 12 that one of them drive roller 15 is connected is fixed can dismantle with the below corresponds from gear 6 and is connected, the outer pad 16 that is fixed with the elastic rubber of round platform shape or silica gel material of wrapping up of drive roller 15, centrifuging tube 19 is pegged graft between a plurality of drive rollers 15 in the one end of centrifugation jack 13, and closely contradict with outer pad 16, be convenient for rotate through driving connecting axle 12 from gear 6, connecting axle 12 drives drive roller 15 and outer 16 rotations of filling up, and then drive roller 15 and outer 16 drive centrifuging tube 19 through filling up 16 and rotate, realize centrifuging tube 19's centrifugal operation, utilize the outer pad 16 of round platform shape can be firm simultaneously to erect centrifuging tube 19 between many drive rollers 15, and then be convenient for drive roller 15 to drive centrifuging tube 19 and. Fixed slot 42 has been seted up on centrifugal seat 2, fixed slot 42 internal fixation has telescopic link 43, the tip that centrifugal seat 2 was kept away from to telescopic link 43 can be dismantled through bolt fastening and be connected with backup pad 44, the one end interference that telescopic link 43 was kept away from to backup pad 44 is connected with stay tube 26, the one end of stay tube 26 runs through the test tube stopper and extends to in centrifuging tube 19, be convenient for link together stay tube 26 and telescopic link 43 through backup pad 44, and then utilize telescopic link 43 to realize the reciprocating of stay tube 26, be convenient for the stay tube 26 to put into centrifuging tube 19 or take out from centrifuging tube 19, be convenient for fixed stay tube 26 simultaneously, make stay tube 26 not influence. The outer side wall of one part of the support tube 26 in the centrifuge tube 19 is detachably connected with a fixing ring 101 through a bolt, the centrifuge tube further comprises a sliding ring 104 which is positioned above the fixing ring 101 and is slidably connected with the outer side wall of the support tube 26, a structure which enables the fixing ring 101 and the sliding ring 104 to be far away is arranged between the sliding ring 104 and the fixing ring 101, for example, a connecting spring 105 or an elastic rope and the like have an elastic structure, a plurality of corresponding lower support rods 102 and upper support rods 103 which are uniformly arrayed in an annular shape are hinged on the outer circumferential surface or the upper edge and the lower edge of the fixing ring 101 and the sliding ring 104, the lower support rods 102 and the upper support rods 103 are hinged, the fixing ring 101 and the sliding ring 104 are conveniently connected together through the hinged connection of the upper support rods 103 and the lower support rods 102, and can move relatively simultaneously, and the centrifuge tube further, when the sliding ring 104 slides along the supporting tube 26, the upper supporting rods 103 rotate around the hinge points with the fixing ring 101 under the driving action of the lower supporting rods 102, so that a structure that the inner filtering membrane 106106 contracts or expands relative to the supporting tube 26 is realized, the structure that the inner filtering membrane 106106 contracts or expands forms a structure that the liquid flowing upwards in the centrifugal tube 19 is released or cut off, and when the liquid flowing upwards is cut off, the inner filtering membrane 106106 gathers the cells with larger diameter or other impurities in the liquid to the central part below the inner filtering membrane 106106 in the centrifugal tube 19. The slip ring 104 is connected with a retraction unit which controls the slip ring 104 to slide along the support tube 26 through a plurality of steel wires 40 outside the centrifuge tube 19, so that the retraction unit can control the slip ring 104 to slide up and down along the support tube 26, and further control the contraction or expansion of the inner filtering membrane 106106. Can dismantle through the screw in the stay tube 26 and be connected with aspiration tube 30 and back flow tube 31, and the tip of back flow tube 31 is less than the tip of aspiration tube 30, a plurality of inlet 32 have been seted up on the stay tube 26 at tip place of aspiration tube in centrifuging tube 19, be convenient for will assemble through aspiration tube 30 and draw out in the liquid of the central part below interior filtration membrane 106106 in centrifuging tube 19 great diameter cell or other debris, utilize back flow tube 31 will be taken out and put into liquid transport meeting centrifuging tube 19 after filtering from aspiration tube 30 simultaneously, guarantee the interior sufficient liquid in centrifuging tube 19 on the right side, liquid can pass through inlet 32 entering stay tube 26 when aspiration tube 30 draws liquid in addition, and then realize that aspiration tube 30 will assemble the cell of the great diameter in the liquid of the central part below interior filtration membrane 106106 in centrifuging tube 19 fast and draw out. The end of the aspiration tube 30 inside the centrifuge tube 19 is located inside the collapsed or expanded configuration of the inner filter membrane 106106 to further facilitate the extraction of larger diameter cells or other debris from the liquid that pools in the center of the centrifuge tube 19 below the inner filter membrane 106106. The end part of the return pipe 31 in the centrifuge tube 19 is on the same horizontal plane with the end part of the support pipe 26 in the centrifuge tube 19, so that the liquid discharged from the return pipe 31 can be conveniently conveyed to the lower end of the centrifuge tube 19, and the influence on the liquid discharged from the return pipe 31 by the liquid pumping pipe 30 to be pumped out immediately and the influence on the liquid pumping pipe 30 to pump cells with larger diameter or other impurities is avoided. The filtering hole diameter of the internal filtering membrane 106106 is 100nm, so that cells or impurities with the diameter exceeding 100nm in the liquid can be conveniently cut into the central part of the centrifugal tube 19 below the internal filtering membrane 106106, and can be conveniently pumped out through the liquid pumping tube 30. The liquid extracting tube 30 and the return tube 31 are respectively communicated with a left flow distributing and collecting valve 33 and a right flow distributing and collecting valve 38, so that the liquid extracting tubes 30 extending out of each centrifuge tube 19 can be conveniently gathered and communicated through the left flow distributing and collecting valve 33, the liquid extracted from the liquid extracting tube 30 and filtered is dispersedly conveyed back to the respective centrifuge tube 19 through the return tube 31 through the right flow distributing and collecting valve 38, and then the circulation of the liquid in the centrifuge tubes 19 is realized. The left flow distributing and collecting valve 33 is communicated with a filter unit for removing cells with larger diameter or other impurities in the liquid through a communicating pipe, so that the liquid extracted from the liquid extracting pipe 30 is conveniently filtered through the filter unit, the cells with larger diameter or other impurities in the liquid are taken out, and the content of the impurities in the obtained exosomes is less. The filter unit is communicated with the microcirculation water pump through a first liquid conveying pipe, the microcirculation water pump is communicated with the right flow distribution and collection valve 38 through a second liquid conveying pipe, so that the microcirculation water pump is communicated with the filter unit and the flow distribution and collection valve through the first liquid conveying pipe and the second liquid conveying pipe, and power is provided for liquid extraction of the liquid extracting pipe 30 and liquid conveying of the return pipe 31.

As shown in figures 1 and 2, the stem cell source exosome rotary separating device is welded from the center of the end face of a gear 6 or detachably connected with a supporting shaft 7 through bolt fixation, the base 1 is welded or detachably connected with a first belt seat bearing 8 through bolt fixation, the supporting shaft 7 is far away from one end of the gear 6 and is in interference connection with the first belt seat bearing 8, the supporting shaft is convenient to support and fix the driven gear 6 through the first belt seat bearing 8 and the supporting shaft 7, and meanwhile, the driving of the main gear 5 is not influenced to rotate from the gear 6. From the welding of 6 another terminal surface axle centers of gear or through bolt fastening detachably connected with connector 9, non-circular spliced eye 10 has been seted up on the connector 9, connecting axle 12 of connecting on one of them drive roller 15 runs through centrifugal seat 2 and extends to from the driving wheel top, and its tip extends has the bayonet joint 11 with 10 looks adaptations of bayonet joint, bayonet joint 11 is pegged graft in bayonet joint 10, be convenient for peg graft through bayonet joint 11 and realize connecting axle 12 and connector 9 joint in bayonet joint 10 and be connected, make to drive connector 9 from gear 6 and rotate, connector 9 drives connecting axle 12 and rotates, connecting axle 12 drives drive roller 15 and rotates, and then realize that drive roller 15 drives centrifuging tube 19 and rotates.

As shown in fig. 1, 3, rotatory separator of stem cell source exosome, it has rotation groove 14 to open in the centrifugation jack 13, the upper and lower inner terminal surface of rotation groove 14 welds respectively or can dismantle through bolt fastening and be connected with second rolling bearing 18 and third rolling bearing 17, the both ends of connecting axle 12 interference connection respectively is on second rolling bearing 18 and third rolling bearing 17, be convenient for support drive roller 15 through second rolling bearing 18 and third rolling bearing 17 and erect in rotation groove 14, and do not influence the normal rotation of drive roller 15, make things convenient for drive roller 15 to drive centrifuging tube 19 and rotate. The quantity of drive roller 15 is 3-6, and 4 drive rollers 15 are best, can enough utilize drive roller 15 to drive centrifuging tube 19 and rotate, also can inject centrifuging tube 19 simultaneously for centrifuging tube 19 can follow vertical direction and peg graft in centrifugation jack 13, avoids centrifuging tube 19 centrifugation effect once of inclining in centrifugation jack 13. The diameter that outer 16 is close to the one end of centrifugation jack 13 is less than the diameter of the other end, and the surface of outer 16 pads is the rough surface, and the centrifuging tube 19 of being convenient for is pegged graft between a plurality of drive rollers 15, and utilizes the frictional force between the outer 16 pads increase of coarse surface and the centrifuging tube 19 for it rotates to make things convenient for more to drive centrifuging tube 19 under the drive of drive roller 15. The centrifugal insertion hole 13 above the rotating groove 14 is of an inverted truncated cone shape, that is, the diameter of the opening of the centrifugal insertion hole 13 is larger than the inner diameter, so that the centrifugal tube 19 can be conveniently inserted into the centrifugal insertion hole 13. The opening part inside wall of centrifugation jack 13 passes through the supporting pad 20 that the adhesive is fixed with elastic rubber or silica gel material, and centrifuging tube 19 is inconsistent with supporting pad 20, is convenient for inject centrifuging tube 19 at the opening part of centrifugation jack 13 through supporting pad 20, avoids centrifuging tube 19 to break away from in centrifugation jack 13 in centrifugal process, also makes centrifuging tube 19's upper end can vertically fix in centrifugation jack 13 simultaneously, avoids 19 slopes of centrifuging tube, influences centrifugal effect.

As shown in fig. 1, 6, 7, and 9, a hole inlet line is provided on the support tube 26 above the sliding ring 104, the steel wire 40 extends into the support tube 26 through the hole inlet line, a hole outlet line is provided at the joint of the support tube 26 and the support plate 44, one end of the steel wire 40 far away from the slide block extends out of the support tube 26 through the hole outlet line, so that the steel wire 40 walks in the support tube 26 through the hole inlet 39 and the hole outlet 41 in one end of the support tube 26 located in the centrifuge tube 19, and the steel wire 40 is prevented from affecting the test tube plug at the opening of the centrifuge tube 19 and the liquid rotation in the centrifuge tube 19. The winding and unwinding unit comprises a reel 303, a positioning wheel 304, a rotating shaft 302, a rotating rod 307 and a pin 313, a winding and unwinding groove 301 is formed in the supporting plate 44, the reel 303 and the positioning wheel 304 are rotatably connected in the winding and unwinding groove 301 through the rotating shaft 302, the reel 303 and the positioning wheel 304 are conveniently arranged in the winding and unwinding groove 301, and the use outside the supporting plate 44 is prevented from being influenced. The supporting plate 44 is provided with a wire feeding hole 45, the steel wire 40 extends into the wire feeding hole 45 from the supporting tube 26 and is fixedly connected with the wire wheel 303 in a tied manner, the steel wire 40 can conveniently travel through the wire feeding hole 45, and the steel wire 40 can be conveniently wound and released through the wire wheel 303. The end of the rotating shaft 302 near the end of the positioning wheel 304 is provided with a telescopic hole 305 along the length direction of the rotating shaft 302, and the rotating rod 307 is movably connected in the telescopic hole 305, so that the rotating rod 307 can rotate and slide in the telescopic hole 305. The inner bottom of the telescopic hole 305 is provided with a non-circular rotating slot 306, one end of the rotating rod 307 in the telescopic hole 305 is fixed with a rotating plug 308 matched with the rotating slot 306, the rotating rod 307 is conveniently connected with the rotating shaft 302 in a clamping manner by being inserted into the rotating slot 306 through the rotating plug 308, and then the rotating rod 307 rotates to drive the rotating shaft 302 to rotate. Rotating on dwang 307 and being connected with and connecting lantern ring 311, slide opening 312 has been seted up on the axis of rotation 302, the one end of pin pole 313 runs through slide opening 312 and is connected lantern ring 311 welded fastening and be connected, make pin pole 313 with be connected lantern ring 311 fixed connection together, and then slide in flexible hole 305 through dwang 307, it removes to drive and connect lantern ring 311 and pin pole 313, do not influence dwang 307 simultaneously again and rotate, and make things convenient for pin pole 313 to remove through slide opening 312, and prescribe a limit to pin pole 313 again, avoid pin pole 313 to rotate along with dwang 307. Connect and weld on the dwang 307 of the both sides of lantern ring 311 or through the fixed first spacing arch 309 of detachably connected with of bolt and second spacing arch 310, be convenient for inject the lantern ring 311 through first spacing arch 309 and second spacing arch 310 for connect lantern ring 311 can remove in the flexible hole 305 along with dwang 307. The pin 313 is T-shaped, and the length of the connection section of the pin 313 and the connection collar 311 is greater than the diameter of the positioning wheel 304, so that the pin 313 is not affected by the positioning wheel 304 when the rotating shaft 302 drives the pin 313 to rotate. The two ends of the pin rod 313 far away from the connecting sleeve ring 311 are respectively welded or detachably connected with a first clamping pin head 314 and a second clamping pin head 315 through bolt fixation, the side wall of the retraction groove 301 near the positioning wheel 304 is provided with a plurality of clamping pin grooves 316 which are matched with the ratchets of the positioning wheel 304 and are uniformly arranged in a ring shape by taking the rotating shaft 302 as the center, when the rotating plug 308 is far away from the rotating slot 306, the first clamping pin head 314 is connected with the ratchets of the positioning wheel 304 in a clamping manner, and the second clamping pin head 315 is connected with the clamping pin grooves 316 in a clamping manner, so that the clamping limitation on the rotating shaft 302 is realized through the connection of the ratchets of the first clamping pin head 314 and the positioning wheel 304 and the clamping pin heads 315 and the clamping pin grooves 316 in a clamping manner, further, the rotation of the wire wheel 303 is avoided, and the upper. When the rotating plug 308 is plugged into the rotating slot 306, the first clamping pin head 314 is far away from the ratchet of the positioning wheel 304, and the second clamping pin head 315 is far away from the clamping pin slot 316, at this time, the rotating rod 307 rotates to drive the rotating shaft 302 to rotate, so that the rotation of the reel 303 is realized. One end of the rotating rod 307 far away from the reel 303 penetrates through the supporting plate 44 and is welded or detachably connected with the rotating wheel 317 through bolt fixation, so that the rotating rod 307 is driven to rotate through the rotating wheel 317. The opening of the retractable groove 301 is fixedly detachably connected with a retractable sealing cover 318 through a bolt, so that the opening of the retractable groove 301 is conveniently sealed through the retractable sealing cover 318, and dust is prevented from entering the retractable groove 301 to influence the normal work of the retractable unit.

As shown in fig. 1, 5 rotatory separator of stem cell source exosome, the test tube stopper passes through the adhesive by rubber buffer 21 and connecting block 22 and fixes or can dismantle through the bolt fastening and connect and constitute, be convenient for through having elastic rubber buffer 21 shutoff centrifuging tube 19's opening, avoid centrifuging tube 19 interior liquid spill in centrifugal process, utilize simultaneously connecting block 22 to be convenient for getting of test tube stopper to put, and be convenient for centrifuging tube 19 drives connecting block 22 and uses stay tube 26 to rotate as the pivot. The axle center of rubber buffer 21 and connecting block 22 has seted up down downthehole 23 and the downthehole 24 of going up that is linked together respectively, is convenient for place stay tube 26 through upturning hole 24 and downthehole 23 down, and does not influence a stay tube 26 of rubber buffer 21 and connecting block 22 and rotates for the pivot. Go up and change hole 24's lateral wall and seted up the arrangement groove 25, be provided with the spacing lantern ring 27 of self-lubricating material in the arrangement groove 25, the supporting tube 26 runs through spacing lantern ring 27, spacing lantern ring 27 is for using solid lubricant graphite base as principal ingredients and nylon, molybdenum disulfide, tungsten disulfide, carbon fiber reinforcing agent and lubricating oil are the composition, through the compounding, the roll is smelted, the shaping, the immersion oil, process such as softening treatment makes, be convenient for reduce the frictional force between supporting tube 26 and the arrangement groove 25 through spacing lantern ring 27, make a supporting tube 26 that the test tube stopper can be better rotate for the pivot. The limiting sealing cover 29 is clamped and fixed at the opening of the placing groove 25, so that the opening of the placing groove 25 is conveniently plugged through the limiting sealing cover 29, the limiting lantern ring 27 is further limited in the placing groove 25, and the limiting lantern ring 27 is prevented from being separated from the placing groove 25. The outer side wall of the supporting tube 26 above the limiting lantern ring 27 is welded or detachably connected with a third limiting protrusion 28 through bolt fixing, so that the supporting tube 26 is limited through the third limiting protrusion 28, and the damage caused by the fact that the lower end of the supporting tube 26 is abutted to the bottom of the centrifuge tube 19 is avoided.

As shown in fig. 1 and 8, the stem cell source exosome rotary separating device comprises a filter box 201, a filter basket 203, an outer filter membrane 204 and a filter cover 205, wherein the inner wall of the filter box 201 is welded or detachably connected with a support block 202 through bolt fixing, the filter basket 203 is connected to the support block 202 in a clamping manner, the support block 202 is used for supporting and limiting the filter basket 203, and the filter basket 203 is convenient to take and place, so that the filter basket 203 is convenient to clean. The outer filtering membrane 204 is detachably connected to the inner end face of the filtering basket 203 through bolt fixation, and the diameter of the filtering hole of the outer filtering membrane 204 is 100nm, so that the liquid discharged from the left flow distributing and collecting valve 33 is conveniently filtered through the outer filtering membrane 204, cells or impurities with the diameter exceeding 100nm in the liquid are removed, and the content of common impurities in exosomes in the centrifuge tube 19 is reduced. The communicating pipe is communicated with the filter box 201 above the filter basket 203, and the first infusion pipe is communicated with the lower end of the side wall of the filter box 201, so that the liquid discharged from the left flow distributing and collecting valve 33 is firstly filtered by the outer filter membrane 204 fixed in the filter basket 203 and then discharged by the first infusion pipe, and the liquid entering the first infusion pipe is ensured not to contain cells or impurities with the diameter exceeding 100 nm. Filter closing cap 205 can dismantle the connection at the opening part of filter cartridge 201 through the fix with screw, and filter closing cap 205 is equipped with sealed the pad with the opening junction of filter cartridge 201, be convenient for seal the closure through the opening that filters closing cap 205 with filter cartridge 201, make the inside confined space that forms of filter cartridge 201, and then be convenient for the microcirculation water pump through first transfer line extraction liquid in from filter cartridge 201, and then make liquid extraction pipe 30 draw liquid to left reposition of redundant personnel collecting valve 33 in from centrifuging tube 19, the rethread communicating pipe is carried in filter cartridge 201.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (9)

1. Stem cell source exosome rotary separating device, including base (1) and centrifugation seat (2), its characterized in that, base (1) internal fixation has centrifugal motor (4), the output shaft fixedly connected with master gear (5) of centrifugal motor (4), master gear (5) rotate and are connected with a plurality of gears (6) from, set up a plurality of centrifugal jack (13) that are the even array of annular grafting centrifuging tube (19) that use the output shaft of centrifugal motor (4) as the center on centrifugation seat (2), rotate through connecting axle (12) in centrifugation jack (13) and be connected with a plurality of drive rollers (15), connecting axle (12) that one of them drive roller (15) is connected can be dismantled with the below is corresponding from gear (6) is fixed and be connected, the outer pad (16) that have the round platform shape of drive roller (15) outer parcel, the one end of centrifuging tube (19) in centrifugation jack (13) is pegged graft between a plurality of drive rollers (15), and closely contradict with outer pad (16), be fixed with backup pad (44) through telescopic link (43) on centrifugal seat (2), be fixed with stay tube (26) on backup pad (44), the one end of stay tube (26) is run through the test tube stopper and is extended to centrifuging tube (19) in, stay tube (26) is fixed with solid fixed ring (101) in centrifuging tube (19) a lateral wall, still including being located gu fixed ring (101) top with stay tube (26) lateral wall sliding connection has slip ring (104), be equipped with between slip ring (104) and solid fixed ring (101) and make gu fixed ring (101) and slip ring (104) keep away from the structure mutually, gu the outer peripheral face of fixed ring (101) and slip ring (104) or border department about articulated have a plurality of corresponding lower support bars (102) and last bracing piece (103) that are the even array of annular, the lower supporting rods (102) are hinged with the upper supporting rods (103), the lower supporting rods further comprise inner filtering membranes (106) (106) which are positioned on the surface of a circular ring formed by each upper supporting rod (103) and are fixed, when the sliding ring (104) slides along the supporting tube (26), each upper supporting rod (103) rotates around a hinge point between the upper supporting rod and the fixing ring (101) under the driving action of each lower supporting rod (102), a structure that the inner filtering membranes (106) (106) shrink or expand relative to the supporting tube (26) is realized, the structure that the inner filtering membranes (106) (106) shrink or expand forms a structure that liquid flowing upwards in the centrifugal tube (19) is released or cut off, and when the liquid flowing upwards is cut off, the inner filtering membranes (106) (106) gather cells with larger diameters or other impurities in the liquid to the central part below the inner filtering membranes (106) (106) in the centrifugal tube (19), the slip ring (104) is connected with a retraction unit which controls the slip ring (104) to slide along the supporting tube (26) outside the centrifugal tube (19) through a plurality of steel wires (40), a liquid extracting pipe (30) and a return pipe (31) are fixed in the supporting pipe (26), the end part of the return pipe (31) is lower than the end part of the liquid pumping pipe (30), the supporting pipe (26) where the end part of the water pumping pipe in the centrifugal pipe (19) is positioned is provided with a plurality of liquid inlets (32), the liquid extracting pipe (30) and the return pipe (31) are respectively communicated with a left flow distributing and collecting valve (33) and a right flow distributing and collecting valve (38), the left flow distributing and collecting valve (33) is communicated with a filtering unit for removing cells with larger diameter or other impurities in the liquid through a communicating pipe, the filter unit is communicated with a micro-circulating water pump through a first liquid conveying pipe, and the micro-circulating water pump is communicated with a right flow distributing and collecting valve (38) through a second liquid conveying pipe.

2. The stem cell source exosome rotary separating device according to claim 1, wherein a supporting shaft (7) is fixed at the center of the end face of the slave gear (6), a first bearing with a seat (8) is fixed on the base (1), one end, far away from the slave gear (6), of the supporting shaft (7) is connected to the first bearing with a seat (8) in an interference mode, a connector (9) is fixed on the axis of the other end face of the slave gear (6), a non-circular insertion hole (10) is formed in the connector (9), a connecting shaft (12) connected to one driving roller (15) penetrates through the centrifugal seat (2) and extends to the position above a driven wheel, an insertion head (11) matched with the insertion hole (10) extends from the end of the connecting shaft, and the insertion head (11) is inserted into the insertion hole (10).

3. The stem cell source exosome rotary separating device according to claim 1, characterized in that a rotation groove (14) is formed in the centrifugal jack (13), the upper and lower inner end faces of the rotation groove (14) are respectively fixed with a second bearing with seat (18) and a third bearing with seat (17), the two ends of the connecting shaft (12) are respectively in interference connection with the second bearing with seat (18) and the third bearing with seat (17), the outer surface of the outer pad (16) is a rough surface, the centrifugal jack (13) above the rotation groove (14) is in a shape of an inverted circular truncated cone, a supporting pad (20) is fixed on the inner side wall of the opening of the centrifugal jack (13), and the centrifugal tube (19) is inconsistent with the supporting pad (20).

4. The stem cell-derived exosome rotating separation device according to claim 1, characterized in that the end of the aspiration tube (30) in the centrifuge tube (19) is located in a structure in which the inner filtration membrane (106) (106) is contracted or expanded, the end of the return tube (31) in the centrifuge tube (19) is on the same level with the end of the support tube (26) in the centrifuge tube (19), and the filtration pore diameter of the inner filtration membrane (106) (106) is 100 nm.

5. The stem cell source exosome rotating separation device according to claim 1, wherein an inlet hole line is arranged on the support tube (26) above the sliding ring (104), the steel wire line (40) extends into the support tube (26) through the inlet hole line, an outlet hole line is arranged at the joint of the support tube (26) and the support plate (44), and one end, far away from the sliding block, of the steel wire line (40) extends out of the support tube (26) through the outlet hole line.

6. The stem cell source exosome rotary separation device according to claim 1, wherein the collecting and releasing unit comprises a wire wheel (303), a positioning wheel (304), a rotating shaft (302), a rotating rod (307) and a pin rod (313), a collecting and releasing groove (301) is formed in the support plate (44), the wire wheel (303) and the positioning wheel (304) are rotatably connected in the collecting and releasing groove (301) through the rotating shaft (302), a wire passing hole (45) is formed in the support plate (44), the steel wire (40) extends into the wire passing hole (45) from the inside of the support tube (26) and is fixedly connected with the wire wheel (303), a telescopic hole (305) is formed in the end part of one end, close to the positioning wheel (304), of the rotating shaft (302) along the length direction of the rotating shaft (302), the rotating rod (307) is movably connected in the telescopic hole (305), a non-circular rotary slot (306) is formed in the inner bottom part of the telescopic hole (305), dwang (307) one end in flexible hole (305) is fixed with the rotation plug (308) with rotating slot (306) looks adaptation, dwang (307) are gone up to rotate and are connected with and connect lantern ring (311), slide opening (312) have been seted up on axis of rotation (302), slide opening (312) and connection lantern ring (311) fixed connection are run through to the one end of round pin pole (313).

7. The stem cell source exosome rotating and separating device according to claim 6, wherein a first limiting protrusion (309) and a second limiting protrusion (310) are fixed on the rotating rod (307) at two sides of the connecting sleeve ring (311), the pin rod (313) is T-shaped, the length of a section of the pin rod (313) connected with the connecting sleeve ring (311) is larger than the diameter of the positioning wheel (304), a first clamping pin head (314) and a second clamping pin head (315) are respectively fixed at two ends of the pin rod (313) far away from the connecting sleeve ring (311), a plurality of clamping pin grooves (316) which are uniformly arranged in an annular shape by taking the rotating shaft (302) as a center and are matched with the ratchet teeth of the positioning wheel (304) are formed on the side wall of the retraction groove (301) close to the positioning wheel (304), and when the rotating plug (308) is far away from the rotating slot (306), the first clamping pin head (314) is connected with the ratchet teeth of the positioning wheel (304) in a clamping manner, the structure that second bayonet catch head (315) and bayonet groove (316) joint are connected, the one end that the line wheel (303) was kept away from in dwang (307) runs through backup pad (44) and is fixed with runner (317), the opening part of receiving and releasing groove (301) is fixed with receives and releases closing cap (318).

8. The stem cell source exosome rotary separating device according to claim 1, characterized in that the test tube plug comprises a rubber plug (21) and a connecting block (22) fixed connection, the axle center of the rubber plug (21) and the connecting block (22) is respectively provided with a lower rotating hole (23) and an upper rotating hole (24) which are communicated, the side wall of the upper rotating hole (24) is provided with a placement groove (25), a limiting sleeve ring (27) made of a self-lubricating material is arranged in the placement groove (25), the supporting tube (26) penetrates through the limiting sleeve ring (27), the opening of the placement groove (25) is fixed with a limiting sealing cover (29), and the outer side wall of the supporting tube (26) above the limiting sleeve ring (27) is fixed with a third limiting protrusion (28).

9. The stem cell source exosome rotary separation device according to claim 1, wherein the filter unit comprises a filter cassette (201), a filter basket (203), an outer filter membrane (204) and a filter cover (205), a support block (202) is fixed on the inner wall of the filter cassette (201), the filter basket (203) is clamped on the support block (202), the outer filter membrane (204) is fixed on the inner end face of the filter basket (203), the diameter of a filter hole of the outer filter membrane (204) is 100nm, the communication pipe is communicated with the filter cassette (201) above the filter basket (203), the first infusion pipe is communicated with the lower end of the side wall of the filter cassette (201), and the filter cover (205) is fixed at the opening of the filter cassette (201).

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