CN213652474U

CN213652474U - Cell exosome extraction element

Info

Publication number: CN213652474U
Application number: CN202021640398.2U
Authority: CN
Inventors: 费方利; 钟丽萍; 范婷
Original assignee: Guangdong Cruilife Stem Cell Technology Co ltd
Current assignee: Guangdong Cruilife Stem Cell Technology Co ltd
Priority date: 2020-08-10
Filing date: 2020-08-10
Publication date: 2021-07-09
Anticipated expiration: 2030-08-10

Abstract

The utility model discloses a cell exosome extraction device, which comprises an outer tube mechanism, wherein an inner tube mechanism is clamped at the inner side of the outer tube mechanism, a first extraction mechanism is clamped at the inner side of the inner tube mechanism, a second extraction mechanism is clamped at the inner side of the first extraction mechanism, the extraction device drives a first extraction tube to be clamped in a second inner groove through the inner tube to drive a second extraction tube to be clamped in a first extraction groove, cell culture supernatant is stored in the second extraction groove, a second filter screen in the second extraction groove is used for filtering, the first filter screen in the first inner groove is used for filtering, then the second filter screen flows into the second inner groove, impurities are filtered into the first inner groove through a third filter screen and are discharged, the separation and extraction are carried out successively for replacement and cleaning, the existing exosome extraction device is effectively realized, the separation and extraction are carried out step by step, the extraction efficiency is higher, extract the exosome purer, extraction element can dismantle simultaneously, is convenient for change and clear up, can circulate and use.

Description

Cell exosome extraction element

Technical Field

The utility model relates to an extraction element, special design cell exosome extraction element belongs to the exosome field.

Background

Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins, and nowadays refer to disc-shaped vesicles with diameters of 40-100nm, which were first discovered in sheep reticulocytes in 1983, and named as "exosomes" by Johnstone in 1987, and which can be secreted by various cells under normal and pathological conditions.

The existing cell exosome extraction method is characterized in that the extraction is carried out by an ultracentrifugation method, but the operation is complex, the cost is too high, meanwhile, the cleaning and extraction structure is inconvenient to replace, and a cell exosome extraction device needs to be designed.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome prior art's cell exosome and draw through the ultracentrifugation method, nevertheless the operation is complicated, and is with too high costs, is not convenient for change simultaneously and washs the defect of extracting structure, provides cell exosome extraction element.

In order to solve the technical problem, the utility model provides a following technical scheme:

the utility model discloses cell exosome extraction element, including outer tube mechanism, the inboard joint of outer tube mechanism has an inner tube mechanism, the inboard joint of inner tube mechanism has a first mechanism that draws, the first inboard joint that draws of mechanism has a second to draw the mechanism.

As a preferred technical scheme of the utility model, outer tube mechanism includes the outer tube, the outer tube inboard is equipped with a first inside groove, a draw-in groove has all been seted up to first inside groove both sides, outer tube upper end joint has a tube cap, a spacing groove has been seted up in the middle of the tube cap, sealed piece of spacing inslot side fixedly connected with.

As a preferred technical scheme of the utility model, inner tube mechanism includes the inner tube, the inner tube joint is inboard in first inside groove, a second inside groove has been seted up to the inner tube inboard, a fixture block of the equal fixedly connected with in inner tube both sides, a first connecting slot has all been seted up to second inside groove both sides inside wall, a third filter screen of inner tube lower extreme fixedly connected with, the third filter screen aperture is 10-40 nm.

As an optimal technical scheme of the utility model, first mechanism of drawing includes first extraction tube, first extraction tube joint is inboard in the second inside groove, first extraction tube inboard is seted up a first extraction groove, first extraction tube both sides fixedly connected with a first connecting block, a second connecting groove has all been seted up to first extraction groove both sides inside wall, a first extraction tube bottom fixedly connected with a first filter screen, first filter screen aperture is 200 thin and thick and has 1000 nm.

As an optimal technical scheme of the utility model, the second draws the mechanism and includes the second extraction tube, the second is drawn the pipe joint and is drawn the inslot side at first, the second is drawn the intraductal side and has been seted up a second and draw the groove, the equal fixedly connected with second connecting block in second extraction tube both sides, a second filter screen of second extraction tube bottom fixedly connected with, second filter screen aperture is 800 + 1500 nm.

As a preferred technical scheme of the utility model, fixture block and draw-in groove joint, first connecting groove and first connecting block joint.

As a preferred technical scheme of the utility model, second connecting block and second spread groove joint.

The utility model discloses the beneficial effect who reaches is: the utility model discloses cell exosome extraction element has easy and simple to handle, is convenient for change the characteristics of clearance extraction structure, in specific use, compares with traditional cell exosome extraction element:

the extraction device drives the inner pipe to be clamped into the first inner groove by clamping the clamping block into the clamping groove, then drives the first extraction pipe to be clamped into the second inner groove by clamping the first connecting block into the first connecting groove, then drives the second extraction pipe to be clamped into the first extraction groove by clamping the second connecting block into the second connecting groove, stores cell culture supernatant into the second extraction groove, filters the cell culture supernatant through the second filter screen in the second extraction groove, filters the cell culture supernatant through the first filter screen in the first inner groove, flows into the second inner groove, filters impurities into the first inner groove through the third filter screen and discharges the impurities out, sequentially separates and extracts the impurities, and then sucks the exosomes separated from the second inner groove by a pipette, namely a required exosome product, and the liquid (extracted exosomes) can be gathered together by the conical design at the lower part of the inner pipe, so that the loss of the trace liquid is not easy to absorb by the pipette head, through taking out first inside groove in proper order with second extraction tube and first extraction tube and inner tube, change the washing, the effectual current exosome extraction element that has realized makes progressively to separate and draws, and extraction efficiency is higher, draws the exosome purer, and extraction element can dismantle simultaneously, is convenient for change and clear up, can carry out recycling.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention.

In the drawings:

fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic structural view of the outer tube of the present invention;

FIG. 3 is a schematic view of the tube cover structure of the present invention;

fig. 4 is a schematic structural view of the inner tube mechanism of the present invention;

fig. 5 is a schematic structural view of a first extraction mechanism of the present invention;

fig. 6 is a schematic structural view of a second extraction mechanism of the present invention.

In the figure: 1. an outer tube mechanism; 11. an outer tube; 12. a first inner tank; 13. a card slot; 14. a tube cover; 15. a limiting groove; 16. a sealing block;

2. an inner tube mechanism; 21. an inner tube; 22. a second inner tank; 23. a clamping block; 24. a first connecting groove; 25. a third filter screen;

3. a first extraction mechanism; 31. a first extraction tube; 32. a first extraction tank; 33. a first connection block; 34. a second connecting groove; 35. a first filter screen;

4. a second extraction mechanism; 41. a second extraction tube; 42. a second extraction tank; 43. a second connecting block; 44. a second filter.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are presented herein only to illustrate and explain the present invention, and not to limit the present invention.

Example 1

As shown in fig. 1-6, the utility model discloses cell exosome extraction element, including outer tube mechanism 1, the inboard joint of outer tube mechanism 1 has an inner tube mechanism 2, and the inboard joint of inner tube mechanism 2 has a first mechanism 3 that draws, and the first 3 inboard joints that draw the mechanism have a second to draw mechanism 4, has played exosome extraction element, makes to separate progressively and draws, and extraction efficiency is higher, draws the exosome purer, and extraction element can dismantle simultaneously, is convenient for change and clear up, can carry out recycle.

Wherein, outer tube mechanism 1 includes outer tube 11, and outer tube 11 inboard is equipped with a first inside groove 12, and a draw-in groove 13 has all been seted up to first inside groove 12 both sides, and outer tube 11 upper end joint has a tube cap 14, has seted up a spacing groove 15 in the middle of the tube cap 14, and a sealing block 16 of the inboard fixedly connected with of spacing groove 15 has played fixed inner tube 21, makes between inner tube 21 and the first inside groove 12 fixed more firm.

Wherein, the inner tube mechanism 2 comprises an inner tube 21, the inner tube 21 is clamped inside the first inner groove 12, the inner side of the inner tube 21 is provided with a second inner groove 22, two sides of the inner tube 21 are fixedly connected with a clamping block 23, two side inner walls of the second inner groove 22 are respectively provided with a first connecting groove 24, the lower end of the inner tube 21 is fixedly connected with a third filter screen 25, the aperture of the third filter screen 25 is 10-40nm, the fixing of the first extraction tube 31 and the second inner groove 22 is realized, and the connection between the second inner groove 22 and the first extraction tube 31 is firmer.

Wherein, the first extraction mechanism 3 includes the first extraction tube 31, the first extraction tube 31 is connected to the inner side of the second inner groove 22 in a clamping manner, the inner side of the first extraction tube 31 is provided with a first extraction groove 32, the two sides of the first extraction tube 31 are fixedly connected with a first connecting block 33, the inner side walls of the two sides of the first extraction groove 32 are respectively provided with a second connecting groove 34, the bottom of the first extraction tube 31 is fixedly connected with a first filter screen 35, the aperture of the first filter screen 35 is 200 and 1000nm, the second extraction tube 41 is fixed, the connection between the second extraction tube 41 and the first extraction tube 31 is firmer, and the exosome is extracted for the first time.

Wherein, the second extraction mechanism 4 comprises a second extraction tube 41, the second extraction tube 41 is clamped inside the first extraction groove 32, the second extraction groove 42 is arranged inside the second extraction tube 41, the two sides of the second extraction tube 41 are fixedly connected with a second connecting block 43, the bottom of the second extraction tube 41 is fixedly connected with a second filter screen 44, the aperture of the second filter screen 44 is 800-1500nm, the second extraction of exosomes is performed, and the exosomes can be extracted more purely.

Wherein, fixture block 23 and draw-in groove 13 joint, first connecting groove 24 and first connecting block 33 joint have played and have made the connection between inner tube 21 and outer tube 11 and the first extraction tube 31 more firm.

The second connecting block 43 is clamped with the second connecting groove 34, so that the first extraction pipe 31 and the second extraction pipe 41 are fixed, and the first extraction pipe 31 and the second extraction pipe 41 are fixed more firmly.

Specifically, when the utility model is used, the sealing block 16 is pulled out from the first inner groove 12 and exposes the first inner groove 12 by pulling the tube cover 14 in the outer tube mechanism 1, the inner tube 21 is driven to be clamped in the first inner groove 12 by clamping the clamping block 23 in the inner tube mechanism 2 into the clamping groove 13, the first connecting block 33 in the first extraction mechanism 3 is clamped in the first connecting groove 24 to drive the first extraction tube 31 to be clamped in the second inner groove 22, the liquid containing exosomes is poured into the second extraction groove 42 in the second extraction mechanism 4, the second connecting block 43 is clamped in the second connecting groove 34 to clamp the second extraction tube 41 into the first extraction groove 32, the tube cover 14 is covered on the upper end of the outer tube 11 to seal the first inner groove 12, the liquid is filtered by the centrifugal force through the second filter screen 44 and then flows into the first extraction groove 32, and then is filtered by the first filter screen 35, then flows into the second inner groove 22, and the impurities flow into the first inner groove 12 and are discharged by extracting the exosomes in the second inner groove 22 by a pipette, namely, the exosome product required by people is obtained, the liquid (extracted exosomes) can be gathered together by the conical design of the lower part of the inner tube 2, a gun head is used for sucking a small amount of liquid, the second extraction tube 41, the first extraction tube 31 and the inner tube 21 are not easy to lose, and then the second extraction tube 41, the first extraction tube 31 and the inner tube 21 are pulled out for cleaning or replacement, so that the existing cell exosome extraction device is effectively realized, the exosomes are extracted by gradually separating, the extraction efficiency is high, the operation is simple, and the extracted exosomes are purer, and meanwhile, the extraction device can be detached, replaced and cleaned, and can be recycled.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Cell exosome extraction element, including outer tube mechanism (1), its characterized in that, outer tube mechanism (1) inboard joint has an inner tube mechanism (2), inner tube mechanism (2) inboard joint has one and first draws mechanism (3), first draw mechanism (3) inboard joint has a second to draw mechanism (4).

2. The extracellular exosome extraction device according to claim 1, wherein the outer tube mechanism (1) comprises an outer tube (11), a first inner groove (12) is formed in the inner side of the outer tube (11), clamping grooves (13) are formed in two sides of the first inner groove (12), a tube cover (14) is clamped at the upper end of the outer tube (11), a limiting groove (15) is formed in the middle of the tube cover (14), and a sealing block (16) is fixedly connected to the inner side of the limiting groove (15).

3. The device for extracting exosomes according to claim 1, wherein the inner tube mechanism (2) comprises an inner tube (21), the inner tube (21) is clamped inside the first inner groove (12), a second inner groove (22) is formed inside the inner tube (21), two sides of the inner tube (21) are fixedly connected with a clamping block (23), two side inner walls of the second inner groove (22) are respectively provided with a first connecting groove (24), a third filter screen (25) is fixedly connected to the lower end of the inner tube (21), and the aperture of the third filter screen (25) is 10-40 nm.

4. The extracellular exosome extraction device according to claim 1, wherein the first extraction mechanism (3) comprises a first extraction tube (31), the first extraction tube (31) is clamped inside the second inner groove (22), a first extraction groove (32) is formed inside the first extraction tube (31), a first connecting block (33) is fixedly connected to two sides of the first extraction tube (31), a second connecting groove (34) is formed in each of the inner side walls of the two sides of the first extraction groove (32), a first filter screen (35) is fixedly connected to the bottom of the first extraction tube (31), and the aperture of the first filter screen (35) is 200-1000 nm.

5. The extracellular exosome extraction device according to claim 1, wherein the second extraction mechanism (4) comprises a second extraction tube (41), the second extraction tube (41) is clamped inside the first extraction groove (32), a second extraction groove (42) is formed inside the second extraction tube (41), two sides of the second extraction tube (41) are fixedly connected with a second connection block (43), the bottom of the second extraction tube (41) is fixedly connected with a second filter screen (44), and the aperture of the second filter screen (44) is 800-1500 nm.

6. The extracellular exosome extraction device according to claim 3, wherein the fixture block (23) is clamped with a fixture groove (13), and the first connection groove (24) is clamped with a first connection block (33).

7. The extracellular exosome extraction device according to claim 5, wherein the second connection block (43) is snap-fitted with a second connection groove (34).