CN216192400U

CN216192400U - Cell filter

Info

Publication number: CN216192400U
Application number: CN202122586966.6U
Authority: CN
Inventors: 冯金华; 皋源; 何征宇; 邢顺鹏; 梅舒雅; 徐侨翌; 汤日; 周洋; 钟晗
Original assignee: Renji Hospital Shanghai Jiaotong University School of Medicine
Current assignee: Renji Hospital Shanghai Jiaotong University School of Medicine
Priority date: 2021-10-26
Filing date: 2021-10-26
Publication date: 2022-04-05
Anticipated expiration: 2031-10-26

Abstract

The utility model relates to a cell filter, and belongs to the technical field of biomedical experimental tools. Comprises a body, a filter screen and an exhaust tube; the body is provided with a channel with two open ends and closed periphery, and the channel is internally provided with a filter screen for separating the channel into two parts; the two parts of the channel are communicated through a filter screen; an exhaust pipe penetrates through the filter screen; a sealing ring is arranged on the inner wall of the channel close to the opening at one end. The utility model provides a convenient and practical cell filter, which can accelerate cell filtration under the condition of filter screens with the same aperture and is simple and convenient to operate.

Description

Cell filter

Technical Field

The utility model relates to a cell filter, and belongs to the technical field of biomedical experimental tools.

Background

In the prior art, during flow cytometry analysis/screening experiments, a single cell sample needs to be extracted through a tissue sample, and in the extraction process, cell masses, tissue fragments and collagen which are not completely dissociated are very easily generated, so that the risk of machine blockage exists; it is generally necessary to filter the cells before further experiments are performed on the machine. The cell filter is a common cell filtering and purifying device, and can filter and separate a single cell digestive juice to obtain a single cell suspension, and can also filter and separate a cell culture medium to obtain the single cell suspension. The cell filter in the prior art is composed of a plastic frame and a screen, and the cell-carrying solution passes through the screen under the action of gravity, while larger tissue fragments cannot pass through the screen, so that the separation of the cells and the tissue fragments is completed. However, the cell filter in the prior art has the following disadvantages: the filter mesh has small diameter and slow cell filtration speed.

Disclosure of Invention

The utility model aims to solve the technical problem of how to improve the cell filtration speed under the condition of a filter screen with the same pore size.

In order to solve the above problems, the technical solution of the present invention is to provide a cell filter, which comprises a body, a filter screen and an air exhaust tube; the body is provided with a channel with two open ends and closed periphery, and the channel is internally provided with a filter screen for separating the channel into two parts; the two parts of the channel are communicated through a filter screen; an exhaust pipe penetrates through the filter screen; a sealing ring is arranged on the inner wall of the channel close to the opening at one end.

Preferably, the body is provided in a cylindrical shape.

Preferably, the plane of the sieve is perpendicular to the central axis of the cylindrical body.

Preferably, an opening at one end of the body is provided with a first annular framework, and the first annular framework is provided with an opening of the channel; the plane of the first annular framework is perpendicular to the central axis of the cylindrical body; an exhaust pipe penetrates through the first annular framework.

Preferably, a second annular framework is arranged between the filter screen and the inner wall of the cylindrical body, and an exhaust pipe penetrates through the second annular framework.

Preferably, a sealing ring and a thread for connection are arranged on the inner wall of the channel close to the opening at the other end of the body.

Preferably, the sealing ring is a rubber ring.

Preferably, the pore size of the filter screen is set to 50 to 100 μm.

Preferably, the outer wall of the body is provided with an anti-slip groove.

Compared with the prior art, the utility model has the following beneficial effects:

the utility model provides a convenient and practical cell filter, which can accelerate cell filtration under the condition of filter screens with the same aperture and is simple and convenient to operate.

Drawings

Fig. 1 is a first structural diagram of the present invention.

FIG. 2 is a second schematic structural diagram of the present invention.

Fig. 3A is a schematic perspective view of the present invention.

FIG. 3B is a schematic side view of the present invention.

FIG. 3C is a schematic top view of the present invention.

Reference numerals: 1. an air exhaust pipe; 2. a ring-shaped framework I; 3. a ring-shaped side wall; 4. a second annular framework; 5. filtering with a screen; 6. an anti-slip groove; 7. a seal ring; 8. and (4) threading.

Detailed Description

In order to make the utility model more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings:

as shown in the attached drawings, the technical scheme adopted by the utility model is to provide a cell filter, which comprises a body, a filter screen 5 and an exhaust tube 1; a channel with two open ends and closed periphery is arranged in the body, and a filter screen 5 for separating the channel into two parts is arranged in the channel; the two parts of the channel are communicated through a filter screen 5; an exhaust pipe 1 penetrates through the filter screen 5; a sealing ring 7 is arranged on the inner wall of the channel close to the opening at one end. The body is cylindrical. The cylindrical body is provided with a ring-shaped side wall 3; the plane in which the sieve 5 lies is perpendicular to the central axis of the cylindrical body. An opening at one end of the body is provided with a ring-shaped framework I2, and the ring-shaped framework I2 is provided with an opening of a channel; the plane of the annular framework I2 is perpendicular to the central axis of the cylindrical body; an exhaust pipe 1 penetrates through the annular framework I2. An annular framework II 4 is arranged between the filter screen 5 and the inner wall of the cylindrical body, and an exhaust pipe 1 penetrates through the annular framework II 4. A sealing ring 7 and a thread 8 for connection are arranged on the inner wall of the channel close to the opening at the other end of the body. The sealing ring 7 is a rubber ring. The pore size of the screen 5 is set to 50-100 μm. The outer wall of the body is provided with an anti-skid groove 6.

Examples

The utility model provides a cell filter, which comprises a ring-shaped side wall 3, wherein the middle part of the ring-shaped side wall 3 forms an upper cavity (a filter cavity) and a lower cavity of the filter through a ring-shaped framework II 4 and a filter screen 5; the lower cavity is composed of a lower half annular side wall 3, a sealing ring 7 and a thread 8. And an exhaust pipe 1 penetrating through the upper cavity (filter cavity) to the lower cavity is further arranged, and the exhaust pipe 1 is fixed through a ring-shaped framework I2 so as to prevent breakage. The whole cell filter is composed of the upper cavity (filter cavity), the lower cavity and the exhaust tube 1.

By adopting the structure, when in use, the cell filter is screwed on the centrifuge tube through the screw thread 8, the lower cavity forms a relatively closed space through the sealing ring 7 and the centrifuge tube, and the only inlet and outlet are the pipelines leading to the filter screen 5 and the exhaust tube 1 on the upper cavity part. After screwing, suspension containing cells is added into the upper cavity (filter cavity), after the suspension covers the filter screen 5, the suspension is sucked through the air exhaust tube 1 by an external ear washing ball, a needle cylinder or a negative pressure aspirator, so that the air pressure in the centrifuge tube is lower than the atmospheric pressure, the suspension containing the cells and tissue fragments is influenced by the air pressure difference, and the suspension rapidly passes through the filter screen 5 to complete the filtration of the single cell suspension.

While the utility model has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the utility model. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.

Claims

1. A cell filter is characterized by comprising a body, a filter screen and an exhaust tube; the body is provided with a channel with two open ends and closed periphery, and the channel is internally provided with a filter screen for separating the channel into two parts; the two parts of the channel are communicated through a filter screen; an exhaust pipe penetrates through the filter screen; a sealing ring is arranged on the inner wall of the channel close to the opening at one end.

2. A cell filter according to claim 1, wherein: the body is cylindrical.

3. A cell filter according to claim 2, wherein: the plane of the filter screen is perpendicular to the central axis of the cylindrical body.

4. A cell filter according to claim 3, wherein: an opening at one end of the body is provided with a first annular framework, and the first annular framework is provided with an opening of the channel; the plane of the first annular framework is perpendicular to the central axis of the cylindrical body; an exhaust pipe penetrates through the first annular framework.

5. A cell filter according to claim 4, wherein: and a second annular framework is arranged between the filter screen and the inner wall of the cylindrical body, and an exhaust pipe penetrates through the second annular framework.

6. A cell filter according to claim 5, wherein: and a sealing ring and a thread for connection are arranged on the inner wall of the channel close to the opening at the other end of the body.

7. A cell filter according to claim 6, wherein: the sealing ring is a rubber ring.

8. A cell filter according to claim 7, wherein: the aperture of the filter screen is set to be 50-100 microns.

9. A cell filter according to claim 8, wherein: and the outer wall of the body is provided with an anti-skid groove.