CN203238262U - Novel negative pressure cell sieve filter device - Google Patents
Novel negative pressure cell sieve filter device Download PDFInfo
- Publication number
- CN203238262U CN203238262U CN 201320102196 CN201320102196U CN203238262U CN 203238262 U CN203238262 U CN 203238262U CN 201320102196 CN201320102196 CN 201320102196 CN 201320102196 U CN201320102196 U CN 201320102196U CN 203238262 U CN203238262 U CN 203238262U
- Authority
- CN
- China
- Prior art keywords
- negative pressure
- cell
- filter
- filtration
- centrifuge tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a novel negative pressure cell sieve filter device. The novel negative pressure cell sieve filter device comprises a filter, a centrifugal tube and a negative pressure generation device, wherein an opening is formed in one end of the centrifugal tube; the filter is arranged at the opening end of the centrifugal tube in a sealed manner; an air exhaust hole of the negative pressure generation device is communicated with the centrifugal tube. During filtration, after air is exhausted from the negative pressure generation device, negative pressure is provided into the centrifugal tube through a negative pressure tube, so that the pressure of the lower surface of a filter screen is smaller than the pressure of the upper surface of the filter screen, and a cell fluid is sucked into the centrifugal tube to complete filtration. According to the novel negative pressure cell sieve filter device, a conventional cell screen filter technology in the technical field of biology is improved; particularly, the filter which is used for primary cell culture, consists of only the filter screen and has a non-ideal filtration effect is eliminated, wherein by the adoption of the filter, a user needs to perform repeated grinding. The problems of difficulty in filtration of tissue homogenate during cell filtration, large operation difficulty, high time consumption, high labor consumption, large cell loss, high pollution probability and the like are solved.
Description
Technical field
The utility model belongs to a kind of cell separation apparatus, is specifically related to the novel negative pressure cell sieve filtration unit of cell filtration and transfer.
Background technology
Cell screening is a kind of indispensable technique means for cell cultures, Celluar and Molecular Biology technology neighborhood.At present, the cell screening technology mainly contains: 1) flow cytometric sorting method, 2) magnetic activated cell seperation, 3) adherent sieve method, 4) density gradient centrifugation.These technique means also exist desirable not to the utmost part in the technical solution problem: 1) although the flow cytometric sorting method is separated rapid for the powder cell, purity is high, but somewhat expensive, the too low trace cell of content is difficult to separate, can not process a large amount of cell samples, it is also very difficult to obtain aseptic cell suspension; 2) magnetic activated cell seperation is larger to the activity influence of cell, and the rate of recovery only has 60%-75%, may cause the cell complete deactivation, and same operation is complicated, expensive; 3) though the adherent sieve method cell screening method that to be present laboratory the most frequently used is consuming time longer, relate to the vitro culture of cell, certain difficulty is arranged during use; 4) density gradient centrifugation is the concentrated granulocytic method of powder commonly used, but need to use separating medium (such as Ficoll or Hypaque liquid etc.), and need at last the flush away separating medium, keep the special equipment of aseptic needs of whole operation, somewhat expensive.
And when at present both at home and abroad using screen filtration, the screen cloth that uses during particularly to primary cell culture is comprised of filter screen merely, the user needs repeatedly to grind, and tissue homogenate still is difficult for filtering, and exists operation easier large, the serious problems such as take time and effort, cell depletion is larger, and opportunities for contamination is larger.Therefore, need to improve existing filtering technique, provide easy to operate, time saving and energy saving and less to cell contamination chance Novel filtration technology.
The utility model content
In view of this, the utility model provides a kind of novel negative pressure cell sieve filtration unit, overcome in the prior art and formed by filter screen merely, need the user repeatedly to grind during filtration, the tissue homogenate still difficult operation easier of going down of filtering is large, the problems such as take time and effort, cell depletion is larger, and opportunities for contamination is larger.
The utility model solves the problems of the technologies described above by following technique means:
A kind of novel negative pressure cell sieve filtration unit comprises strainer, also comprises centrifuge tube and depression generator, described centrifuge tube one end opening, and described filter seal is arranged at the opening end of centrifuge tube, and the bleeding point of described depression generator is communicated with centrifuge tube.
Further, described depression generator comprises negative tube and air extractor, and the bleeding point of described negative tube one end passes the inside that the outer wall that filters cavity extend into centrifuge tube vertically, and the other end mouth of pipe is communicated with air extractor.
Further, described strainer comprises filter screen and filters cavity that the edge of described filter screen is connected in the filtration cavity inner wall.
Further, the capacity of described centrifuge tube is the 40-60 milliliter.
The beneficial effects of the utility model: negative pressure cell sieve filtration unit of the present utility model, comprise strainer, also comprise centrifuge tube and depression generator, described centrifuge tube one end opening, described filter seal is arranged at the opening end of centrifuge tube, and the bleeding point of described depression generator is communicated with centrifuge tube.During filtration, after depression generator is bled by negative tube to negative pressure is provided in the centrifuge tube so that filter screen lower surface pressure is less than upper surface pressure, thereby will finish filtration behind enchylema " suction " centrifuge tube.The utility model has improved the traditional cell screen filtration technology of biological technical field, uses when particularly having improved primary cell culture to be comprised of filter screen merely, needs the user repeatedly to grind, and the unsatisfactory strainer of filter effect.The problems such as tissue homogenate is difficult for filtering when also having overcome cell filtration, and operation easier is large, takes time and effort, and cell depletion is larger, and contamination probability is larger.It is easy to operate that the utility model provides, time saving and energy saving and less to cell contamination chance Novel filtration technology.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further described.
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the synoptic diagram of filter screen.
Embodiment
Below with reference to accompanying drawing the utility model is elaborated, as shown in the figure: the novel negative pressure cell sieve filtration unit of present embodiment, comprise strainer 1, also comprise centrifuge tube 2 and depression generator 3, one end opening of described centrifuge tube 2, described strainer 1 sealing is arranged at the opening end of centrifuge tube 2, and the bleeding point of described depression generator 3 is communicated with centrifuge tube 2.During filtration, after depression generator is bled by negative tube to negative pressure is provided in the centrifuge tube so that filter screen lower surface pressure is less than upper surface pressure, thereby will finish filtration behind enchylema " suction " centrifuge tube.
As further improvement in the technical proposal, described depression generator 3 comprises negative tube and air extractor, the bleeding point of described negative tube one end passes the inside that the outer wall that filters cavity extend into centrifuge tube 2 vertically, perhaps directly pass the inside that centrifugal tube wall extend into centrifuge tube 2, the other end is communicated with the air extractor sealing, and air extractor can adopt syringe, pump bowl or any bleeder equipment that other satisfies condition.
As further improvement in the technical proposal, described strainer 1 comprises filter screen and filters cavity, described filter screen edge connection filtration cavity inner wall, and filter screen can be arranged at bottom or the middle and lower part of filtering cavity.
As further improvement in the technical proposal, the capacity of described centrifuge tube 4 is the 40-60 milliliter, and is preferred, gets 50 milliliters, is used for filling the enchylema after storage is filtered.
Adopt the concrete explanation principle of work of the present utility model of syringe and technique means with air extractor: during filtration, syringe bleed after by negative tube to step-down in the centrifuge tube, so that filter screen lower surface pressure is less than normal atmosphere, namely less than filter screen upper surface pressure, owing between the filter screen upper and lower surface pressure difference is arranged, thereby for traditional strainer that does not have pressure reduction, easily with enchylema " suction " centrifuge tube, the gas that syringe is taken away is more, pressure difference between the filter screen upper and lower surface is just larger, and enchylema is just easier by " suction " centrifuge tube.Finish filtration after filtrate passes completely through filter screen, enters centrifuge tube, centrifuge tube should avoid enchylema to enter negative tube as an interim container that stores the enchylema after filtering.
Explanation is at last, above embodiment is only unrestricted in order to the technical solution of the utility model to be described, although with reference to preferred embodiment the utility model is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (4)
1. a novel negative pressure cell sieves filtration unit, comprise strainer (1), it is characterized in that: also comprise centrifuge tube (2) and depression generator (3), one end opening of described centrifuge tube (2), described strainer (1) sealing is arranged at the opening end of centrifuge tube (2), and the bleeding point of described depression generator (3) is communicated with centrifuge tube (2).
2. novel negative pressure cell according to claim 1 sieves filtration unit, it is characterized in that: described depression generator (3) comprises negative tube and air extractor, the bleeding point of described negative tube one end passes the inside that the outer wall that filters cavity stretches into centrifuge tube (2) vertically, and the other end mouth of pipe is communicated with air extractor.
3. novel negative pressure cell according to claim 2 sieves filtration unit, it is characterized in that: described strainer (1) comprises filter screen and filters cavity that described filter screen edge is connected in the filtration cavity inner wall.
4. novel negative pressure cell according to claim 3 sieves filtration unit, and it is characterized in that: the capacity of described centrifuge tube (2) is the 40-60 milliliter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320102196 CN203238262U (en) | 2013-03-06 | 2013-03-06 | Novel negative pressure cell sieve filter device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320102196 CN203238262U (en) | 2013-03-06 | 2013-03-06 | Novel negative pressure cell sieve filter device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203238262U true CN203238262U (en) | 2013-10-16 |
Family
ID=49315579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320102196 Expired - Fee Related CN203238262U (en) | 2013-03-06 | 2013-03-06 | Novel negative pressure cell sieve filter device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203238262U (en) |
-
2013
- 2013-03-06 CN CN 201320102196 patent/CN203238262U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204261563U (en) | The aseptic micro-pore-film filtration device of a kind of veterinary drug injection | |
| CN204543732U (en) | New ceramics filter | |
| CN203238262U (en) | Novel negative pressure cell sieve filter device | |
| CN205730515U (en) | A kind of Simple decompression defecator | |
| CN204827908U (en) | Oil well oil -well pump assistor | |
| CN202185161U (en) | Recycling flash distillation device | |
| CN112834305B (en) | Method and device for efficiently separating enriched cells through continuous flow | |
| CN208883829U (en) | Vinous fermentation pressure setting | |
| CN204981708U (en) | Separator that industrialization of glossy ganoderma spore polysaccharide was drawed | |
| CN202921059U (en) | Solid-liquid separation type bag-type filtering machine | |
| CN204364972U (en) | Electric pneumatic type lautertuns | |
| CN205164234U (en) | Add hot type decompression filter equipment | |
| CN209078367U (en) | A kind of oil liquid separator of purifying cutting fluid device | |
| CN206996003U (en) | A kind of multistage rapid filtering device | |
| CN216192400U (en) | Cell filter | |
| CN201586419U (en) | Protein recovery plant in starch sugar production | |
| CN208949276U (en) | A kind of microbial culture medium filter device | |
| CN207210416U (en) | A kind of universal single cell suspension preparation facilities | |
| CN204891460U (en) | Just, clear up dust remover filter equipment | |
| CN212188014U (en) | Suction filtration device is used in pyrazole alcohol production | |
| CN204093316U (en) | A kind of ultrafiltration test tube Suction filtration device | |
| CN102429610A (en) | Gas-dust separation device | |
| CN218035981U (en) | Device for efficiently extracting seawater micro-plastic | |
| CN208161130U (en) | A kind of Bioexperiment funnel | |
| CN213466861U (en) | Gas-liquid separator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131016 Termination date: 20140306 |