CN213555802U

CN213555802U - Separation device for fluorescent microsphere preparation process

Info

Publication number: CN213555802U
Application number: CN202022423385.6U
Authority: CN
Inventors: 王海燕
Original assignee: Hangzhou Yuanyan Technology Co ltd
Current assignee: Hangzhou Yuanyan Technology Co ltd
Priority date: 2020-10-27
Filing date: 2020-10-27
Publication date: 2021-06-29
Anticipated expiration: 2030-10-27

Abstract

The utility model relates to the technical field of fluorescent microsphere preparation technology, in particular to a separation device for fluorescent microsphere preparation technology; including the separation barrel, the upper and lower both ends of separation barrel all can be dismantled and be connected with sealed lid, are located be equipped with the feed liquor pipe on the sealed lid of separation barrel upper end, are located be equipped with the drain pipe on the sealed lid of separation barrel lower extreme, be equipped with a plurality of ceramic filter assembly in the separation barrel, the output of feed liquor pipe inserts and is located inside the ceramic filter assembly of separation barrel the top, every ceramic filter assembly side all is equipped with the collecting pipe, the output of collecting pipe all runs through the separation barrel setting, and is adjacent can dismantle the connection between the ceramic filter assembly, is located sliding connection between ceramic filter assembly of separation barrel lower extreme and the separation barrel inner wall. The utility model has the advantages of long service life, no by-product in the separation process and environmental friendliness.

Description

Separation device for fluorescent microsphere preparation process

Technical Field

The utility model relates to a preparation technology technical field of fluorescence microballon, in particular to separation device for fluorescence microballon preparation technology.

Background

Since the 80 s in the 20 th century, the rapid development of polymer nanotechnology has promoted its wide application in the field of biotechnology, and functional nanomaterials such as nanospheres, magnetic microspheres, fluorescent microspheres, and colored microspheres have been mainly used in different technical fields. In the preparation process of the fluorescent microsphere, when the fluorescent dye and the polystyrene microsphere are reacted to form the fluorescent microsphere, an excessive mode of the fluorescent dye is generally selected, and how to completely separate the excessive fluorescent dye from the fluorescent microsphere becomes a difficult point in the separation technology.

Currently there are three main methods for separation:

(1) taking supernatant suspension to remove dye precipitate by a natural sedimentation method;

(2) filtering at normal pressure by qualitative filter paper to remove excessive fluorescent dye;

(3) the reaction solution is washed by selecting an organic solvent which can be dissolved in the fluorescent dye but can not dissolve the fluorescent microspheres, and then the dye solution is removed by centrifugation, so that the fluorescent microspheres achieve the purposes of separation and purification.

Although the three separation methods have the advantages, the method also has the defects that the method cannot compensate:

(1) although the natural sedimentation method is very simple and convenient in operation and does not need any auxiliary instrument or equipment, the main disadvantage is that only a few excess dyes with larger specific gravity or larger particles can be removed, and the dyes with relatively lighter specific gravity but not completely precipitated cannot be completely separated, so that the obtained fluorescent microsphere emulsion still contains a lot of dyes, and the subsequent application of the fluorescent microsphere emulsion in the biological field is limited;

(2) qualitative filter paper filtration method. The common qualitative filter paper has three types of fast speed, medium speed and slow speed, the pore sizes of the qualitative filter paper are respectively about 80-120 microns, 30-50 microns and 1-3 microns, but the pore size distribution is not uniform. When the fluorescent microspheres are separated and filtered under normal pressure, a better result is not obtained, although obviously granular excessive dye is removed, the existence of obvious free dye can be seen through microscope observation;

(3) organic solvent washing method. Although the method can well remove the excessive fluorescent dye, the requirement on the selection of the organic solvent is high, and the requirement that the dye can be dissolved and the fluorescent microsphere is not dissolved is met. This can only be achieved with specific fluorochromes, but is not applicable to most fluorochromes, and the separation and purification process uses organic solvents, which are harmful to the environment.

Therefore, a separation device for a fluorescent microsphere preparation process is provided.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a fluorescence microballon is separator for preparation technology, the device have long service life, separation process do not have the by-product and advantage friendly to the environment.

In order to achieve the above purpose, the technical solution of the present invention is as follows:

a separation device for a fluorescent microsphere preparation process comprises a separation barrel body, wherein the upper end and the lower end of the separation barrel body are both of an open structure, the upper end and the lower end of the separation barrel body are both detachably connected with sealing covers, a liquid inlet pipe is arranged on the sealing cover positioned at the upper end of the separation barrel body, a liquid outlet pipe is arranged on the sealing cover positioned at the lower end of the separation barrel body, a water pump and a controller are arranged on the outer side of the separation barrel body, the output end of the water pump is connected with the input end of the liquid inlet pipe, a plurality of ceramic filtering components are arranged in the separation barrel body, the output end of the liquid inlet pipe is inserted into the ceramic filtering components positioned at the uppermost end of the separation barrel body, a collecting pipe is arranged on the side surface of each ceramic filtering component, the output end of each collecting pipe penetrates through the separation barrel, the liquid inlet pipe, the collecting pipe and the liquid outlet pipe are all provided with electromagnetic valves, and the electromagnetic valves and the water pump are respectively electrically connected with the controller.

Specifically, the inner side wall of the sealing cover is provided with internal threads, and the upper end and the lower end of the separation barrel are provided with thread sections matched with the thread sections on the sealing cover.

Specifically, the top surface of the interior of the sealing cover is also provided with a layer of sealing gasket.

Specifically, every ceramic filter component includes ceramic support frame, millipore filtration membrane and filter screen, millipore filtration membrane inlays inside ceramic support frame, the filter screen sets up in the millipore filtration membrane outside, ceramic support frame is cylindrical structure.

Specifically, the inner wall of the separation cylinder is symmetrically provided with a pair of slide rails, the ceramic filtering component positioned at the lowermost end of the separation cylinder further comprises two slide blocks matched with the pair of slide rails, the two slide blocks are symmetrically arranged on the outer side of the ceramic support frame positioned at the lowermost end of the separation cylinder along a longitudinal axis method, and the lowermost end of each slide rail is provided with a stop block.

Specifically, the adjacent ceramic support frames are inserted.

Specifically, the upper end of the ceramic support frame is arranged in a plurality of slots, and the lower end of the ceramic support frame is provided with an insertion block matched with the slots at the upper end of the ceramic support frame.

Specifically, a sealing ring is arranged at the joint of the separation cylinder and the collecting pipe.

The utility model has the advantages that: the utility model has the advantages that the sealing cover, the separation barrel and the sealing cover are detachably connected, so that the sealing cover is convenient to install, disassemble and clean, the use is convenient, and the operation is flexible; by arranging the ceramic filtering components and the water pumps, the fluorescent dye solution can be pressed into the separating cylinder at high pressure for rapid separation and filtration, the filtered fluorescent dye is discharged from the collecting pipe, and the filtered aqueous solution is discharged from the liquid outlet pipe, so that the pressure resistance and impact resistance of the ceramic filtering components can be effectively improved, the service life of the ceramic filtering components is prolonged, no organic solvent is required to be added, no by-product is generated in the separation process, and the ceramic filtering components are environment-friendly; through can dismantle between the adjacent ceramic filter assembly and be located the ceramic filter assembly and the sliding connection between the separation barrel inner wall of separation barrel lowermost end, be convenient for installation, dismantlement and washing ceramic filter assembly, convenient operation.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a ceramic filter assembly according to an embodiment of the present invention.

Reference numerals: the device comprises a separation cylinder body 1, a sealing cover 2, a liquid inlet pipe 3, a liquid outlet pipe 4, a water pump 5, a controller 6, a sealing gasket 7, internal threads 8, a collecting pipe 9, a microporous filter membrane 10, a filter screen 11, a ceramic support frame 12, a slide rail 13, a slide block 14, a stop block 15, an insert block 16, a slot 17 and an electromagnetic valve 18.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to the attached drawing 1, the separating device for the fluorescent microsphere preparation process comprises a separating cylinder body 1, wherein the upper end and the lower end of the separating cylinder body 1 are both of an open structure, the upper end and the lower end of the separating cylinder body 1 are both detachably connected with sealing covers 2, a liquid inlet pipe 3 is arranged on the sealing cover 2 positioned at the upper end of the separating cylinder body 1, a liquid outlet pipe 4 is arranged on the sealing cover 2 positioned at the lower end of the separating cylinder body 1, a water pump 5 and a controller 6 are arranged at the outer side of the separating cylinder body 1, the output end of the water pump 5 is connected with the input end of the liquid inlet pipe 3, a plurality of ceramic filtering components are arranged in the separating cylinder body 1, the output end of the liquid inlet pipe 3 is inserted into the ceramic filtering component positioned at the uppermost end of the separating cylinder body 1, a, the adjacent ceramic filtering components are detachably connected, the ceramic filtering component positioned at the lowest end of the separation barrel body 1 is in sliding connection with the inner wall of the separation barrel body 1, the liquid inlet pipe 3, the collecting pipe 9 and the liquid outlet pipe 4 are respectively provided with the electromagnetic valve 18, and the electromagnetic valve 18 and the water pump 5 are respectively electrically connected with the controller 6; by arranging the ceramic filtering components and the water pump 5, the fluorescent dye solution can be pressed into the separating cylinder 1 at high pressure for rapid separation and filtration, the filtered fluorescent dye is discharged from the collecting pipe 9, and the filtered water solution is discharged from the liquid outlet pipe 4, so that the pressure resistance and impact resistance of the ceramic filtering components can be effectively improved, the service life of the ceramic filtering components is prolonged, no organic solvent is required to be added, no by-product is generated in the separation process, and the ceramic filtering components are environment-friendly; through can dismantle between the adjacent ceramic filtering component and connect and lie in between the ceramic filtering component of separation barrel 1 bottommost and the sliding connection of separation barrel 1 inner wall, be convenient for install, dismantle and wash ceramic filtering component, convenient operation.

Specifically, the inner side wall of the sealing cover 2 is provided with an internal thread 8, and the upper end and the lower end of the separation cylinder 1 are provided with thread sections matched with the thread sections on the sealing cover 2; the top surface of the inside of the sealing cover 2 is also provided with a layer of sealing gasket 7; the separation cylinder body 1 is detachably connected with the sealing cover 2, so that the sealing cover 2 is convenient to mount, dismount and clean, the use is convenient, and the operation is flexible; by providing the gasket 7, a sealing function can be achieved.

Specifically, referring to fig. 2, each of the ceramic filter assemblies includes a ceramic support frame 12, a microporous filter membrane 10 and a filter screen 11, the microporous filter membrane 10 is embedded in the ceramic support frame 12, the filter screen 11 is disposed on the outer side of the microporous filter membrane 10, and the ceramic support frame 12 is a cylindrical structure; the microporous filter membrane 10 is embedded in the ceramic support frame 12 by adopting an embedded structure, and the filter screen 11 is arranged outside the microporous filter membrane 10, so that the pressure resistance and the shock resistance of the ceramic filter component can be effectively improved, and the service life of the ceramic filter component is prolonged.

Specifically, a pair of slide rails 13 is symmetrically arranged on the inner wall of the separation cylinder 1, the ceramic filter assembly located at the lowermost end of the separation cylinder 1 further comprises two slide blocks 14 matched with the pair of slide rails 13, the two slide blocks 14 are symmetrically arranged on the outer side of the ceramic support frame 12 located at the lowermost end of the separation cylinder 1 along a longitudinal axis method, a stop block 15 is arranged at the lowermost end of the slide rails 13, the use position of the ceramic support frame 12 located at the lowermost end of the separation cylinder 1 can be limited by the stop block 15, and the ceramic filter assembly is conveniently installed, detached and cleaned through sliding connection between the ceramic filter assembly located at the lowermost end of the separation cylinder 1 and the inner wall of the separation cylinder 1, so that the.

It is specific, adjacent peg graft between the ceramic support frame 12, the upper end of ceramic support frame 12 sets up in a plurality of slots 17, the lower extreme of ceramic support frame 12 be provided with ceramic support frame 12 upper end slot 17 assorted inserted block 16, be convenient for install, dismantle and wash ceramic filter assembly, convenient operation.

Specifically, a sealing ring is arranged at the joint of the separation cylinder body 1 and the collecting pipe 9, so that a sealing effect is achieved.

Further, the microporous membrane 10 of the present invention includes but is not limited to microporous membranes 10 with different pore sizes such as 0.22 μm, 0.45 μm, 0.6-0.8 μm, and 3-10 μm.

Further, the utility model relates to a ceramic filter assembly's quantity is 7, and wherein, from top to bottom the inside millipore filtration membrane 10 apertures of ceramic filter assembly reduce in proper order.

The utility model discloses a theory of operation: when the fluorescent dye separating device is used, a fluorescent dye solution is pressed into the separating cylinder 1 through the water pump 5, the fluorescent dye is remained in the ceramic filtering component under the action of high pressure and is discharged through the collecting pipe 9, and the filtered water solution is discharged through the liquid outlet pipe 4.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. A separation device for a fluorescent microsphere preparation process comprises a separation barrel body and is characterized in that the upper end and the lower end of the separation barrel body are both of an open structure, the upper end and the lower end of the separation barrel body are both detachably connected with sealing covers, a liquid inlet pipe is arranged on the sealing cover positioned at the upper end of the separation barrel body, a liquid outlet pipe is arranged on the sealing cover positioned at the lower end of the separation barrel body, a water pump and a controller are arranged on the outer side of the separation barrel body, the output end of the water pump is connected with the input end of the liquid inlet pipe, a plurality of ceramic filter assemblies are arranged in the separation barrel body, the output end of the liquid inlet pipe is inserted into the ceramic filter assembly positioned at the uppermost end of the separation barrel body, a collecting pipe is arranged on the side surface of each ceramic filter assembly, the output ends of the collecting pipes penetrate through the separation barrel body, the liquid inlet pipe, the collecting pipe and the liquid outlet pipe are all provided with electromagnetic valves, and the electromagnetic valves and the water pump are respectively electrically connected with the controller.

2. The separation device for the preparation process of fluorescent microspheres according to claim 1, wherein the inner side wall of the sealing cover is provided with internal threads, and the upper end and the lower end of the separation cylinder are provided with thread sections matched with the thread sections on the sealing cover.

3. The separation device for fluorescent microsphere preparation process as claimed in claim 2, wherein the top surface of the inside of the sealing cover is further provided with a layer of sealing gasket.

4. The separation device for the preparation process of the fluorescent microspheres according to claim 1, wherein each ceramic filter assembly comprises a ceramic support frame, a microporous filter membrane and a filter screen, the microporous filter membrane is embedded in the ceramic support frame, the filter screen is arranged on the outer side of the microporous filter membrane, and the ceramic support frame is of a cylindrical structure.

5. The separation device for the fluorescent microsphere preparation process according to claim 4, wherein a pair of slide rails is symmetrically arranged on the inner wall of the separation cylinder, the ceramic filter assembly at the lowermost end of the separation cylinder further comprises two slide blocks matched with the pair of slide rails, the two slide blocks are symmetrically arranged on the outer side of the ceramic support frame at the lowermost end of the separation cylinder along the longitudinal axis, and the stop block is arranged at the lowermost end of the slide rails.

6. The separation device for fluorescent microsphere preparation process according to claim 5, wherein adjacent ceramic support frames are inserted.

7. The separation device for the preparation process of fluorescent microspheres according to claim 6, wherein the upper end of the ceramic support frame is arranged in a plurality of slots, and the lower end of the ceramic support frame is provided with an insert matched with the slots at the upper end of the ceramic support frame.

8. The separation device for the fluorescent microsphere preparation process according to claim 1, wherein a sealing ring is arranged at the joint of the separation cylinder and the collection pipe.