CN214974898U

CN214974898U - Fluorescence technology device for rapidly screening monoclonal antibodies

Info

Publication number: CN214974898U
Application number: CN202121131855.XU
Authority: CN
Inventors: 邓杰
Original assignee: Aidi Biotechnology Beijing Co ltd
Current assignee: Aidi Biotechnology Beijing Co ltd
Priority date: 2021-05-25
Filing date: 2021-05-25
Publication date: 2021-12-03
Anticipated expiration: 2031-05-25

Abstract

The utility model discloses a fluorescence technology device for monoclonal antibody filters fast, the power distribution box comprises a box body, the first bevel gear of motor left end drive end fixedly connected with, first bevel gear top meshing is connected with second bevel gear, the rotatory container of cylinder piece top fixedly connected with, the baffle top is provided with centrifugal cup, centrifugal cup top is through branch fixedly connected with centrifugal wall, fixed block top right side is connected with the apron through the pivot rotation, apron bottom fixedly connected with flat bearing. The utility model discloses in, drive rotary container through motor drive and rotate, reach the purpose of centrifugal screening, be provided with a plurality of centrifugal cups through upper portion in the rotary container, improve work efficiency, the rubber pad is pressed and is moved centrifugal cup and move down when installing the operation, seals centrifugal cup top, makes and can not have liquid to spill over when the centrifugation, and the completion of protection centrifugal liquid is worth wideling popularize.

Description

Fluorescence technology device for rapidly screening monoclonal antibodies

Technical Field

The utility model relates to a monoclonal antibody screening technical field especially relates to a fluorescence technology device that is used for monoclonal antibody to filter fast.

Background

The monoclonal antibody is produced by artificially prepared hybridoma cells, the hybridoma cells are formed by fusing B cells activated by antigens and myeloma cells, and the monoclonal antibody has the advantages of high purity, high sensitivity, strong specificity, less cross reaction and low preparation cost.

The monoclonal antibody has important effects in testing diagnostic reagents, purifying proteins and guiding treatment of tumors, but the steps in the screening process are complicated, the manual operation efficiency is low, a large amount of time is consumed, errors are easy to occur, fluorescent markers are needed to be adopted in the screening process, the screening speed can be greatly improved, the top cover is not tight when the existing centrifugal machine is used for centrifugation, and liquid is easy to overflow from the top to cause machine pollution.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a fluorescence technology device for rapidly screening monoclonal antibodies.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a fluorescence technology device for rapidly screening monoclonal antibodies comprises a box body, wherein a bracket is fixedly connected to the right side of the inner bottom wall of the box body, the top end of the bracket is fixedly connected with a motor, the left end driving end of the motor is fixedly connected with a first bevel gear, the top end of the first bevel gear is engaged with a second bevel gear, the middle part of the second bevel gear is fixedly connected with a cylindrical block, the top end of the cylindrical block is fixedly connected with a rotary container, the upper part in the rotary container is uniformly distributed with slots, the inner bottom walls of the slots are fixedly connected with springs, the top ends of the springs are fixedly connected with partition plates, the top ends of the partition plates are respectively provided with a centrifugal cup, the top ends of the centrifugal cups are fixedly connected with a centrifugal wall through a support rod, the box top left side fixedly connected with fixed block, fixed block top right side is rotated through the pivot and is connected with the apron, apron bottom fixedly connected with flat bearing.

As a further description of the above technical solution:

the centrifugal cups are all connected with the slots in a sliding manner.

As a further description of the above technical solution:

and filter holes are uniformly distributed on the surface of the centrifugal wall.

As a further description of the above technical solution:

and an ultrafiltration membrane is attached to the inner surface of the centrifugal wall.

As a further description of the above technical solution:

the rotary container is rotatably connected with the box body.

As a further description of the above technical solution:

the bottom end of the plane bearing is fixedly connected with a rubber pad.

As a further description of the above technical solution:

the supporting rods are distributed at three equal intervals.

As a further description of the above technical solution:

the inner sides of the supporting rods are fixedly connected to the periphery of the top end of the centrifugal wall.

The utility model discloses following beneficial effect has:

1. the utility model discloses in, at first be provided with the motor through the box bottom, motor drive drives rotary container and rotates, reaches the purpose of centrifugal screening, is provided with a plurality of centrifugal cups through upper portion in the rotary container, makes the instrument can carry out the centrifugation to a plurality of centrifugal cups simultaneously at the during operation, improves work efficiency, saves time, reaches the purpose of quickly separating screening.

2. The utility model discloses in, be provided with flat bearing through the apron bottom, flat bearing bottom fixedly connected with rubber pad, centrifugal bottom of cup portion is provided with the spring, and centrifugal cup can slide along the fluting inner wall, and the rubber pad is pressed centrifugal cup and is moved down when the device operation, seals centrifugal cup top, makes and can not have liquid to spill over when the centrifugation, effectively protects the machine to and the complete of protection centrifugal liquid, be worth wideling popularize.

Drawings

FIG. 1 is a front view of a fluorescence technique device for rapid screening of monoclonal antibodies according to the present invention;

FIG. 2 is a perspective view of a centrifuge cup of the fluorescence technique device for rapid screening of monoclonal antibodies according to the present invention;

fig. 3 is a perspective view of a fluorescence technique device for rapidly screening monoclonal antibodies according to the present invention.

Illustration of the drawings:

1. a box body; 2. a second bevel gear; 3. grooving; 4. a spring; 5. rotating the container; 6. a fixed block; 7. a rotating shaft; 8. a cover plate; 9. a flat bearing; 10. a rubber pad; 11. filtering holes; 12. a strut; 13. ultrafiltration membranes; 14. a centrifugal wall; 15. a partition plate; 16. a centrifuge cup; 17. a cylindrical block; 18. a first bevel gear; 19. a motor; 20. and (4) a bracket.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, the present invention provides an embodiment: a fluorescence technology device for rapidly screening monoclonal antibodies comprises a box body 1, a support 20 is fixedly connected to the right side of the inner bottom wall of the box body 1, the support 20 is used for supporting a motor 19 to enable the motor 19 to stably run, the motor 19 is fixedly connected to the top end of the support 20 and is a driving source to drive a rotary container 5 to rotate, a first bevel gear 18 is fixedly connected to the left end driving end of the motor 19, a second bevel gear 2 is connected to the top end of the first bevel gear 18 in a meshed mode and is connected with the top end of the first bevel gear 2 through a bevel gear, the transmission direction is changed, a cylindrical block 17 is fixedly connected to the middle of the second bevel gear 2, the cylindrical block 17 is connected with the second bevel gear 2 and plays a transitional connection role with the rotary container 5, the rotary container 5 is fixedly connected to the top end of the cylindrical block 17, centrifugation is started through the rotation of the rotary container 5, slots 3 are uniformly distributed on the inner upper portion of the rotary container 5, and springs 4 are fixedly connected to the inner bottom walls of the slots 3, 4 equal fixedly connected with baffles 15 in top of spring, 15 tops of baffles all are provided with

centrifugal cup

16, 12 fixedly connected with centrifugal wall 14 are passed through on the 16 tops of centrifugal cup, 1 top left side fixedly connected with fixed block 6 of box, fixed block 6 is used for

fixed cover plate

8, 6 top right sides of fixed block are rotated through pivot 7 and are connected with

apron

8, 8 bottom fixedly connected with flat bearing 9 of apron, flat bearing 9 follows 5 synchronous rotations of rotary container, make 16 tops of centrifugal cup be in the state of tightly closing all the time.

Centrifugal cup 16 all with fluting 3 sliding connection, 14 surface evenly distributed of centrifugal wall has filtration pore 11, the centrifugate that is filtered flows out from

filtration pore

11, 14 internal surfaces of centrifugal wall have laminating milipore filter 13, milipore filter 13 is used for filtering the screening by fluorescence labeling's monoclonal antibody, rotary container 5 rotates with box 1 to be connected, flat bearing 9 bottom fixedly connected with rubber pad 10, rubber pad 10 is used for sealing the 16 rim of a cup of centrifugal cup, branch 12 has three and equal interval distribution, the equal fixed connection in the inboard periphery in 14 tops of centrifugal wall of branch 12.

The working principle is as follows: in the monoclonal antibody screening process, a motor 19 is arranged at the bottom end of a box body 1, the motor 19 runs to drive a rotating container 5 to rotate through bevel gear transmission to achieve the purpose of centrifugal screening, a plurality of centrifugal cups 16 are arranged at the upper part in the rotating container 5 to enable an instrument to centrifuge a plurality of centrifugal cups 16 simultaneously during working, the working efficiency is improved, and the purpose of rapid separation screening is achieved, a plane bearing 9 is arranged at the bottom end of a cover plate 8, a rubber pad 10 is fixedly connected with the bottom end of the plane bearing 9, when the device runs, the cover plate 8 is covered, the rubber pad 10 presses the centrifugal cups 16, the centrifugal cups 16 press a spring 4 to move downwards, the purpose of tightly closing the top ends of the centrifugal cups 16 is achieved, liquid cannot overflow during centrifugation, the machine is effectively protected, the integrity of the centrifugal liquid is protected, an ultrafiltration membrane 13 is attached to the inner wall of the centrifugal wall 14, and monoclonal antibodies to be screened are placed into the centrifugal wall 14 during centrifugation, after centrifugation, the monoclonal antibody which needs to be kept and is marked by fluorescence is kept in the centrifugal wall 14, the screening is rapid, the efficiency is high, and the method is worthy of great popularization.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A fluorescence technology device for monoclonal antibody rapid screening, includes box (1), its characterized in that: the right side of the inner bottom wall of the box body (1) is fixedly connected with a support (20), the top end of the support (20) is fixedly connected with a motor (19), a driving end at the left end of the motor (19) is fixedly connected with a first bevel gear (18), the top end of the first bevel gear (18) is connected with a second bevel gear (2) in a meshing manner, the middle part of the second bevel gear (2) is fixedly connected with a cylindrical block (17), the top end of the cylindrical block (17) is fixedly connected with a rotary container (5), slots (3) are uniformly distributed at the upper part in the rotary container (5), the inner bottom walls of the slots (3) are fixedly connected with springs (4), the top ends of the springs (4) are fixedly connected with partition plates (15), centrifugal cups (16) are arranged at the top ends of the partition plates (15), and centrifugal walls (14) are fixedly connected with the top ends of the centrifugal cups (16) through support rods (12), the box body (1) top left side fixedly connected with fixed block (6), fixed block (6) top right side is rotated through pivot (7) and is connected with apron (8), apron (8) bottom fixedly connected with flat bearing (9).

2. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the centrifugal cups (16) are all connected with the slots (3) in a sliding manner.

3. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the surface of the centrifugal wall (14) is uniformly distributed with filter holes (11).

4. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: and an ultrafiltration membrane (13) is attached to the inner surface of the centrifugal wall (14).

5. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the rotary container (5) is rotatably connected with the box body (1).

6. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the bottom end of the plane bearing (9) is fixedly connected with a rubber pad (10).

7. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the supporting rods (12) are distributed at three equal intervals.

8. The fluorescence technology device for rapidly screening monoclonal antibodies according to claim 1, wherein: the inner sides of the supporting rods (12) are fixedly connected to the periphery of the top end of the centrifugal wall (14).