CN215162615U - Single cell suspension preparation facilities - Google Patents
Single cell suspension preparation facilities Download PDFInfo
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- CN215162615U CN215162615U CN202121268973.5U CN202121268973U CN215162615U CN 215162615 U CN215162615 U CN 215162615U CN 202121268973 U CN202121268973 U CN 202121268973U CN 215162615 U CN215162615 U CN 215162615U
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Abstract
The utility model relates to a unicellular suspension preparation technical field specifically is a unicellular suspension preparation facilities, including the mounting panel, be fixed with two preparation section of thick bamboos that are the symmetric distribution on the mounting panel, be provided with the collar in the preparation section of thick bamboo, be fixed with cell filtration membrane in the collar, the preparation section of thick bamboo is close to bottom position department and is provided with the agitator disk, is fixed with the separation blade on the circumference outer wall of agitator disk, and is fixed with the connecting rod on the roof of agitator disk, the upper end of connecting rod pass the mount and with between the mount sliding connection. The utility model discloses a two preparation section of thick bamboos that can move simultaneously can once only prepare the unicellular suspension of two differences, set up the filtration membrane that can go up and down and agitator disk in the preparation section of thick bamboo simultaneously, can go on simultaneously with two steps of filtration, have solved general unicellular suspension preparation in-process, and efficiency is lower, and separation step and filtration step often separately go on, cause the contaminated problem of suspension easily.
Description
Technical Field
The utility model relates to a unicellular suspension preparation technical field specifically is a unicellular suspension preparation facilities.
Background
In the flow cytometry or magnetic bead sorting and other medical detection, tissue fragments and cell clusters in a sample need to be removed, so that a single cell suspension is obtained, but in the general single cell suspension preparation process, the efficiency is low, and the separation step and the filtration step are often carried out separately, so that the suspension is easily polluted. In view of this, we propose a single cell suspension preparation device.
SUMMERY OF THE UTILITY MODEL
In order to make up for the above deficiency, the utility model provides a unicellular suspension preparation facilities.
The technical scheme of the utility model is that:
the utility model provides a unicellular suspension preparation facilities, includes the mounting panel, the roof four corners position of mounting panel all is fixed with the dead lever, the top of dead lever is fixed with the backup pad, be fixed with two preparation section of thick bamboos that are the symmetric distribution on the mounting panel, be provided with the collar in the preparation section of thick bamboo, the collar internal fixation has cell filtration membrane, just be fixed with the mount on the collar, the preparation section of thick bamboo is close to the bottom and is located position department is provided with the agitator disk under the collar, be fixed with the separation blade on the circumference outer wall of agitator disk, just be fixed with the connecting rod on the roof of agitator disk, the upper end of connecting rod is passed mount and between the mount sliding connection.
Preferably, the upper end of the preparation cylinder is connected with a threaded top cover in a threaded manner, a pair of limiting rods are fixed on the top wall of the mounting ring, the upper ends of the limiting rods penetrate through the threaded top cover and the supporting plate, and a top plate is fixed at the top ends of the limiting rods.
According to a preferable technical scheme, the two top plates are fixedly connected through a connecting plate, a micro motor is installed in the middle of the top wall of the supporting plate, a threaded rod is fixed to an output shaft of the micro motor, and the threaded rod penetrates through the connecting plate and is in threaded connection with the connecting plate.
Preferably, a cleaning brush is fixed to a top wall of the separation blade, and the cleaning brush abuts against a bottom wall of the cell filtration membrane.
According to a preferable technical scheme, the upper end of the connecting rod penetrates through the threaded top cover, gears are fixed on the outer wall of the connecting rod, which is located outside, and the two gears are in transmission through chains.
As a preferred technical scheme, a placing plate is arranged right below the mounting plate and between the two preparation cylinders, a supporting rod is fixed on the top wall of the placing plate, and the supporting rod is fixedly connected with the bottom wall of the mounting plate.
As a preferable technical scheme, the circumferential outer wall of the mounting ring is abutted against the inner wall of the preparation cylinder, and a rubber layer is tightly adhered to the outer wall of the mounting ring.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a two preparation section of thick bamboos that can move simultaneously can once only prepare the unicellular suspension of two differences, set up the filtration membrane that can go up and down and agitator disk in the preparation section of thick bamboo simultaneously, can go on simultaneously with two steps of filtration, have solved general unicellular suspension preparation in-process, and efficiency is lower, and separation step and filtration step often separately go on, cause the contaminated problem of suspension easily.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is a front view of the present invention;
fig. 3 is a sectional view of the preparation cylinder of the present invention.
In the figure: mounting plate 1, fixing rod 11, support plate 2, preparation cylinder 3, threaded top cover 31, mounting ring 32, cell filtration membrane 321, fixing frame 322, limiting rod 323, top plate 324, connecting plate 3241, stirring disc 33, separating blade 331, cleaning brush 3311, connecting rod 332, gear 3321, micromotor 4, threaded rod 41, placing plate 5 and support rod 51.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Referring to fig. 1-3, the present invention provides a technical solution:
the utility model provides a unicellular suspension preparation facilities, including mounting panel 1, the roof four corners position of mounting panel 1 all is fixed with dead lever 11, the top of dead lever 11 is fixed with backup pad 2, be fixed with two preparation section of thick bamboo 3 that are the symmetric distribution on mounting panel 1, be provided with collar 32 in the preparation section of thick bamboo 3, collar 32 internal fixation has cell filtration membrane 321, and be fixed with mount 322 on the collar 32, preparation section of thick bamboo 3 is close to the bottom and is located collar 32 under the position department be provided with agitator disk 33, be fixed with separation blade 331 on the circumference outer wall of agitator disk 33, and be fixed with connecting rod 332 on the roof of agitator disk 33, the upper end of connecting rod 332 pass mount 322 and with mount 322 between sliding connection.
In this embodiment, can separate group's biological tissue through separation blade 331, cell filtration membrane 321 that can go up and down simultaneously filters the separation to the biological tissue after separating, and the extraction is located the unicellular suspension of cell filtration membrane 321 top afterwards can, and in the whole process, reduced with external contact, can effectively prevent that suspension from being contaminated.
Further, the upper end of the preparation cylinder 3 is connected with a threaded top cover 31 in a threaded manner, a pair of limiting rods 323 are fixed on the top wall of the mounting ring 32, the upper ends of the limiting rods 323 penetrate through the threaded top cover 31 and the support plate 2, and a top plate 324 is fixed at the top ends of the limiting rods 323; the screw top cover 31 is used for sealing the preparation cylinder 3 and reducing the contact with the outside, and the limiting rod 323 is used for controlling the ascending or descending of the mounting ring 32, so that the success of the preparation of the single-cell suspension is ensured.
Furthermore, the two top plates 324 are fixedly connected through a connecting plate 3241, the micro motor 4 is installed in the middle of the top wall of the support plate 2, a threaded rod 41 is fixed on an output shaft of the micro motor 4, and the threaded rod 41 penetrates through the connecting plate 3241 and is in threaded connection with the connecting plate 3241; the micro motor 4 provides power to drive the threaded rod 41, the threaded rod 41 rotates to drive the connecting plate 3241 to ascend or descend, and stability and uniform speed of the installation ring 32 in the ascending or descending process are further ensured.
Further, a cleaning brush 3311 is fixed on the top wall of the separation blade 331, and the cleaning brush 3311 abuts against the bottom wall of the cell filtration membrane 321; in an actual preparation process, in order to ensure that the cell filtering membrane 321 can effectively and stably filter the sediment and the cell tissue, the cleaning brush 3311 can continuously clean the bottom wall of the cell filtering membrane 321 when the separation blade 331 rotates to separate the biological tissue, so as to prevent the sediment from accumulating to block the cell filtering membrane 321.
In this embodiment, a connecting rod 332 is fixed on the top wall of the stirring plate 33, the upper end of the connecting rod 332 penetrates through the threaded top cover 31, a gear 3321 is fixed on the outer wall of the connecting rod 332 located outside, and the two gears 3321 are in transmission through a chain; by driving one of the connecting rods 332 to rotate and driving the other connecting rod 332 to rotate, two single-cell suspensions can be prepared at the same time.
In the embodiment, a placing plate 5 is arranged right below the mounting plate 1 and between the two preparation cylinders 3, a supporting rod 51 is fixed on the top wall of the placing plate 5, and the supporting rod 51 is fixedly connected with the bottom wall of the mounting plate 1; the placing plate 5 is provided with test tubes for placing the prepared single-cell suspension.
It is to be noted that a suction pump, not shown, is mounted on the top wall of the mounting plate 1, and the filtered single-cell suspension is sucked into the test tubes on the placing plate 5 by the suction pump.
In addition, the circumferential outer wall of the mounting ring 32 is abutted against the inner wall of the preparation cylinder 3, and a rubber layer is tightly adhered to the outer wall of the mounting ring 32; ensures that the separated biological tissue fluid can be positioned above the mounting ring 32 only after being filtered by the cell filtering membrane 321 in the descending process of the mounting ring 32, and ensures the quality of the single cell suspension.
The utility model discloses a unicellular suspension preparation facilities is when using, at first place the biological tissue that two needs to separate respectively in two preparation section of thick bamboo 3 and be located collar 32 below position, drive one of them connecting rod 332 and rotate, under the cooperation of chain and gear 3321, two connecting rods 332 rotate simultaneously, agitator disk 33, separation blade 331 rotates and carries out broken separation to biological tissue, open micro motor 4, micro motor 4 provides power drive threaded rod 41, threaded rod 41 rotates, drive connecting plate 3241, collar 32, cell filtration membrane 321 descends and filters, open the suction pump and place the test tube on the board 5 with the suction of filtered unicellular suspension, in this process, clearance brush 3311 constantly clears up the diapire of cell filtration membrane 321, the general preparation in-process of unicellular suspension has been solved, the efficiency is lower, and separation step and filtration step often separately go on, easily causing the problem of contamination of the suspension.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A single cell suspension preparation device, comprising a mounting plate (1), characterized in that: the roof four corners position of mounting panel (1) all is fixed with dead lever (11), the top of dead lever (11) is fixed with backup pad (2), be fixed with two preparation section of thick bamboo (3) that are the symmetric distribution on mounting panel (1), be provided with collar (32) in preparation section of thick bamboo (3), collar (32) internal fixation has cell filtration membrane (321), just be fixed with mount (322) on collar (32), preparation section of thick bamboo (3) are close to the bottom and are located position department is provided with agitator disk (33) under collar (32), be fixed with separation blade (331) on the circumference outer wall of agitator disk (33), just be fixed with connecting rod (332) on the roof of agitator disk (33), the upper end of connecting rod (332) is passed sliding connection between mount (322) and mount (322).
2. The single cell suspension preparation device of claim 1, wherein: the upper end of the preparation cylinder (3) is in threaded connection with a threaded top cover (31), a pair of limiting rods (323) are fixed on the top wall of the mounting ring (32), the upper ends of the limiting rods (323) penetrate through the threaded top cover (31) and the supporting plate (2), and a top plate (324) is fixed at the top ends of the limiting rods (323).
3. The single cell suspension preparation device of claim 2, wherein: two roof (324) between through connecting plate (3241) fixed connection, just micromotor (4) are installed to the roof intermediate position of backup pad (2), be fixed with threaded rod (41) on the output shaft of micromotor (4), threaded rod (41) pass connecting plate (3241) and with between connecting plate (3241) threaded connection.
4. The single cell suspension preparation device of claim 1, wherein: a cleaning brush (3311) is fixed on the top wall of the separation blade (331), and the cleaning brush (3311) is abutted against the bottom wall of the cell filtering membrane (321).
5. The single cell suspension preparation device of claim 2, wherein: the upper end of the connecting rod (332) penetrates through the threaded top cover (31), a gear (3321) is fixed on the outer wall of the connecting rod (332) located outside, and the two gears (3321) are in chain transmission.
6. The single cell suspension preparation device of claim 1, wherein: just be located two under mounting panel (1) position department between preparation section of thick bamboo (3) is provided with places board (5), be fixed with branch (51) on placing the roof of board (5), branch (51) with the diapire fixed connection of mounting panel (1).
7. The single cell suspension preparation device of claim 1, wherein: the circumferential outer wall of the mounting ring (32) is abutted against the inner wall of the preparation cylinder (3), and a rubber layer is tightly bonded on the outer wall of the mounting ring (32).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121268973.5U CN215162615U (en) | 2021-06-08 | 2021-06-08 | Single cell suspension preparation facilities |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121268973.5U CN215162615U (en) | 2021-06-08 | 2021-06-08 | Single cell suspension preparation facilities |
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| Publication Number | Publication Date |
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| CN215162615U true CN215162615U (en) | 2021-12-14 |
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| CN202121268973.5U Active CN215162615U (en) | 2021-06-08 | 2021-06-08 | Single cell suspension preparation facilities |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022227354A1 (en) * | 2021-04-29 | 2022-11-03 | 深圳市瑞沃德生命科技有限公司 | Experimental container and biological sample preparation device |
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2021
- 2021-06-08 CN CN202121268973.5U patent/CN215162615U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022227354A1 (en) * | 2021-04-29 | 2022-11-03 | 深圳市瑞沃德生命科技有限公司 | Experimental container and biological sample preparation device |
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Effective date of registration: 20230316 Address after: 610, Floor 6, Block A, No. 2, Lize Middle Second Road, Chaoyang District, Beijing 100102 Patentee after: Zhongguancun Technology Leasing Co.,Ltd. Address before: Room 613-1, building 1, China Agricultural University international entrepreneurship Park, No. 10, Tianxiu Road, Haidian District, Beijing 100193 Patentee before: BEIJING GEEKGENE TECHNOLOGY Co.,Ltd. |