CN210620768U

CN210620768U - Separation device for separating decidua from chorion of placenta wall

Info

Publication number: CN210620768U
Application number: CN201921277469.4U
Authority: CN
Inventors: 江嘉豪; 何美弟; 李静静; 赵蓝
Original assignee: Guangdong Vitalife Biotechnology Co ltd
Current assignee: Guangdong Vitalife Biotechnology Co ltd
Priority date: 2019-08-06
Filing date: 2019-08-06
Publication date: 2020-05-26
Anticipated expiration: 2029-08-06

Abstract

The utility model relates to a separator of separation placenta wall decidua and placenta chorion, include: the inner part of the outer cover is provided with a first containing cavity; the screen body is rotatably arranged in the containing cavity, the bottom of the screen body is higher than the bottom wall of the first containing cavity, a second containing cavity is arranged in the screen body, and sieve holes are formed in the side wall of the second containing cavity; the stirrer is arranged in the sieve body. According to the separation device for separating the decidua on the placenta wall and the placenta chorion, the placenta tissue is placed into the second containing cavity of the sieve body, the stirrer can stir and chop the placenta tissue when the sieve body and the stirrer rotate, the decidua on the placenta wall is thrown out of the sieve pores of the sieve body by using centrifugal force when the sieve body rotates, and the chorion cannot be thrown out of the sieve body due to the fact that the thickness of the chorion is large, so that the decidua on the placenta wall and the placenta chorion can be conveniently and quickly separated.

Description

Separation device for separating decidua from chorion of placenta wall

Technical Field

The utility model belongs to the biological equipment field, concretely relates to separator of separation placenta wall decidua and placenta chorion.

Background

The placenta plays an important role in fetal development, contains a large amount of trophoblasts, abundant blood vessels and mesenchymal components, and is a stem/progenitor cell stock. The placenta has wide source, convenient material taking, no need of invasive operation and no ethical moral problem, and the mesenchymal stem cells extracted from the placenta have great differentiation potential and strong proliferation capacity and are ideal carriers for cell and gene therapy. The mesenchymal stem cells from placenta wall decidua become a research hotspot of tissue engineering due to the advantages of stronger self-renewal, high proliferation and multidirectional differentiation potential, convenient material taking, low immunogenicity, no ethical problem and the like.

However, the traditional method for manually peeling the decidua of the placenta wall from the chorion of the placenta by using tweezers is low in efficiency and easy to carry other tissues, so that the cell types are not unique.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a separator of separation placental wall decidua and placenta chorion, can be fast, conveniently with the separation of placental wall decidua and placenta chorion.

Therefore, the utility model provides a separation device of separation placenta wall decidua and placenta chorion, include:

the inner part of the outer cover is provided with a first containing cavity;

the screen body is rotatably arranged in the containing cavity, the bottom of the screen body is higher than the bottom wall of the first containing cavity, a second containing cavity is arranged in the screen body, and sieve holes are formed in the side wall of the second containing cavity;

the stirrer is arranged in the sieve body and used for stirring placenta tissues when the sieve body rotates so as to separate decidua on the placenta wall from chorion of the placenta, and the decidua on the placenta wall is thrown out of the sieve holes to the first accommodating cavity under the rotating action of the sieve body.

Preferably, the outer cover is a cylinder made of a glass material, and the screen body is a cylinder made of an iron material.

Preferably, the cover body is detachably covered on the opening part of the first cavity.

Preferably, the diameter of the sieve holes is 1.5-2.5 mm.

Preferably, the device further comprises a base, and the outer cover is fixed on the base.

Preferably, the bottom pillar is further included, one end of the bottom pillar is connected to the bottom of the screen body, and the other end of the bottom pillar is connected to the bottom wall of the first accommodating cavity, so that the bottom of the screen body is higher than the bottom wall of the first accommodating cavity.

Preferably, the base includes a rotating motor, and the rotating motor is connected to the screen body to drive the screen body to rotate in the first cavity.

Preferably, the bottom surface of the first cavity further comprises a digestive juice outlet, and the base is provided with a digestive juice inlet communicated with the digestive juice outlet.

Preferably, a temperature controller and an infrared height sensor are arranged in the first accommodating cavity, the temperature controller is used for adjusting and monitoring the temperature of the first accommodating cavity, and the infrared height sensor is used for detecting the liquid level height in the first accommodating cavity.

Preferably, the base still includes controller and display screen, the controller respectively with rotate motor, temperature modulator, infrared altitude sensor and display screen and be connected for control the display screen shows temperature information, time information and the rotational speed of rotating the motor, in the liquid level height in first appearance chamber one or more.

Compared with the prior art, the placenta tissue is placed in the second containing cavity of the sieve body, the stirrer can stir and chop the placenta tissue when the sieve body and the stirrer rotate, the decidua on the wall is thrown out of the sieve holes of the sieve body by using centrifugal force when the sieve body rotates, and the chorion cannot be thrown out of the sieve body due to the fact that the thickness of the chorion is large, so that the decidua on the placenta wall and the chorion of the placenta can be conveniently and quickly separated.

Drawings

Fig. 1 is a schematic diagram of the structure of a separation device for separating decidua from chorion of placenta wall.

In the figure:

1-a cover body; 2-a sieve body; 3-a housing; 4-temperature regulator; 5-a stirrer; 6-bottom pillar; 7-digestive juice outlet; 8-a base; 9-digestive juice inlet; 10-display screen.

Detailed Description

The present invention will be further explained with reference to the accompanying drawings.

Fig. 1 is a schematic diagram of the structure of a separation device for separating decidua from chorion of placenta wall. As shown in fig. 1, the separation device for separating the decidua of the placenta wall and the chorion of the placenta comprises a cover body 1, an outer cover 3, a screen body 2, a stirrer 5, a bottom column 6 and a base 8.

The outer cover 3 is fixed on the base 8, a first accommodating cavity with an open top surface is arranged inside the base, and the cover body 1 detachably covers the open area of the first accommodating cavity to prevent tissues from being separated from the first accommodating cavity. In this embodiment, the outer cover 3 is a cylinder made of glass material, which can prevent the decidua of the placenta wall from being thrown out of the separator and collecting the decidua of the placenta wall.

The screen body 2 is a cylinder made of iron materials. The screen frame 2 is rotationally arranged in the containing cavity, a second containing cavity is arranged in the screen frame 2, the side wall of the second containing cavity is provided with screen holes, and the diameters of the screen holes are 1.5-2.5 mm, preferably 2 mm. The screen frame 2 can make the tissue get rid of to the lateral wall through the centrifugation when rotatory, filters out the placenta wall decidua through the sieve mesh.

The stirrer 5 is arranged in the sieve body 2 and used for stirring placenta tissues when the sieve body 2 rotates to separate the decidua on the placenta wall from the chorion of the placenta, and the decidua on the placenta wall is thrown out of the sieve holes to the first accommodating cavity under the rotating action of the sieve body 2. In this embodiment, a temperature controller 4 and an infrared height sensor are arranged in the first accommodating cavity, the temperature controller 4 is used for adjusting and monitoring the temperature of the first accommodating cavity, and the infrared height sensor is used for detecting the liquid level height in the first accommodating cavity. An infrared height sensor may be provided in the bottom pillar 6 for monitoring the volume of the digestive juice and giving an alarm when the level is too high.

One end of the bottom column 6 is connected to the bottom of the screen body 2, and the other end of the bottom column is connected to the bottom wall of the first accommodating cavity, so that the bottom of the screen body 2 is higher than the bottom wall of the first accommodating cavity. The sieve body 2 can be held to the foundation 6, lets sieve body 2 and the diapire in first appearance chamber have certain difference in height, makes the placenta wall decidua that separates digest under sieve body 2.

The base 8 comprises a rotating motor, and the rotating motor is connected to the screen body 2 to drive the screen body 2 to rotate in the first accommodating cavity. The bottom surface of the first cavity further comprises a digestive juice outlet 7, and the base 8 is provided with a digestive juice inlet 9 communicated with the digestive juice outlet 7. The digestive juice may enter the first chamber via the digestive juice inlet 9 and the digestive juice outlet 7. The base 8 further comprises a controller and a display screen 10, wherein the controller is respectively connected with the rotating motor, the temperature regulator 4, the infrared height sensor and the display screen 10 and is used for controlling the display screen 10 to display one or more of temperature information, time information, the rotating speed of the rotating motor and the liquid level height of the first accommodating cavity. In this embodiment, the display screen 10 may display the quality of the separated decidua of the placenta wall, the rotational speed of the oscillation, and the time of the oscillation.

When the placenta protecting liquid is used in operation, placenta tissue of fetus produced by caesarean section in full term is collected in hospital, the placenta tissue is transported to a laboratory in a refrigerated and sterile environment at 4 ℃, and the tissue protecting liquid is used in the transportation process to protect the biological activity of the placenta tissue. The tissue protection solution is prepared by adding 25ug/ml gentamicin sulfate and 5ug/ml amphotericin B into normal saline, so as to ensure no bacterial and fungal contamination during transportation.

Then, the placenta chorion with the decidua of the placenta wall is cut into blocks in an aseptic laboratory and is put into a separating device to be stripped. After the placenta chorion with the placenta decidua on the placenta wall is put into the separating device, the separating device is started, the stirrer 5 and the screen body 2 rotate simultaneously, the stirrer 5 can further cut the placenta tissue, the screen body 2 rotates to throw the decidua on the placenta wall out of the screen body 2 from the screen holes of the screen body 2 by centrifugal force, and the chorion cannot be thrown out of the screen body 2 due to the large thickness of the chorion.

Then, after the separator is started for a period of time, the decidua on the placenta wall is separated out of the screen body 2, then digestive juice is injected into the first containing cavity through the digestive juice inlet 9 and the digestive juice outlet 7, and the temperature, the oscillation rotating speed and the oscillation time are set to digest the decidua on the placenta wall.

After renting, adding a culture medium with the same volume as the digestive juice after digestion is finished to terminate digestion, and then carrying out subsequent culture.

The placenta tissue is placed into the second containing cavity of the screen body 2, when the screen body 2 and the stirrer 5 rotate, the stirrer 5 can stir and chop the placenta tissue, when the screen body 2 rotates, the decidua on the wall is thrown out of the sieve pores of the screen body 2 by using centrifugal force, and because the thickness of the chorion is larger, the chorion cannot be thrown out of the screen body 2, the decidua on the placenta wall and the chorion of the placenta can be conveniently and quickly separated, and the separation efficiency and the uniqueness of the separated tissues are higher than those of the traditional separation method.

It is to be understood that the present invention is not limited to the above-described embodiments, and various changes and modifications may be made without departing from the spirit and scope of the present invention, and it is intended to cover such changes and modifications if they fall within the scope of the claims and their equivalents.

Claims

1. A separation device for separating decidua from chorion of placenta wall, comprising:

the inner part of the outer cover is provided with a first containing cavity;

2. The separation device of claim 1, wherein said outer cover is a cylinder made of glass material and said screen is a cylinder made of iron material.

3. The placental wall decidua and placental chorion separation device according to claim 2, further comprising a cover removably covering said open portion of said first cavity.

4. A separation device for separating placental wall decidua and placental chorion according to claim 3, wherein said mesh has a diameter of 1.5 to 2.5 mm.

5. The placental wall decidua and placental chorion separation device according to claim 4, further comprising a base, said cover being affixed to said base.

6. The separation device of claim 5, further comprising a bottom pillar having one end connected to the bottom of the screen body and the other end connected to the bottom wall of the first receptacle, such that the bottom of the screen body is higher than the bottom wall of the first receptacle.

7. The separation device of claim 6, wherein said base comprises a rotary motor connected to said screen for rotating said screen within said first receptacle.

8. The placental wall decidua and placental chorion separation device according to claim 7, wherein said first chamber further comprises a digester effluent outlet in a bottom surface thereof, said base being provided with a digester effluent inlet in communication with said digester effluent outlet.

9. The separation device for separating the decidua of the placental wall and the placental chorion according to claim 8, wherein a temperature controller and an infrared height sensor are disposed in said first cavity, said temperature controller is configured to adjust and monitor the temperature of said first cavity, and said infrared height sensor is configured to detect the liquid level in said first cavity.

10. The separation device for separating the decidua of the placental wall and the placental chorion according to claim 9, wherein said base further comprises a controller and a display screen, said controller is connected to said rotating motor, said temperature regulator, said infrared height sensor and said display screen respectively, for controlling said display screen to display one or more of temperature information, time information, and a rotation speed of said rotating motor, and a liquid level of said first chamber.