CN212293597U

CN212293597U - Preparation device of skin cell suspension

Info

Publication number: CN212293597U
Application number: CN202020798723.1U
Authority: CN
Inventors: 李洪生
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-14
Filing date: 2020-05-14
Publication date: 2021-01-05
Anticipated expiration: 2030-05-14

Abstract

The utility model relates to a skin cell suspension's preparation ware, including preparation ware casing and setting up the operation panel on the preparation ware casing, be provided with digestion tank, washing tank and suspension preparation pond on the operation panel, the digestion tank is used for digesting skin tissue, the washing tank is used for washing the skin tissue after the digestion, the suspension preparation pond is used for preparing skin cell suspension; the first filter and the second filter are detachably arranged in the digestion tank and the suspension preparation tank respectively and respectively comprise an outer sleeve and an inner core barrel, and the outer sleeve is sleeved outside the inner core barrel in an annular mode. The utility model discloses the device lets clinician can prepare skin cell suspension fast, high-efficiently in the operating room, directly is used for the treatment of skin defect disease, does not need complicated laboratory personnel and equipment, and operation process is simple, quick, microbial contamination's such as greatly reduced bacterium risk.

Description

Preparation device of skin cell suspension

Technical Field

The utility model relates to a skin cell suspension's preparation ware belongs to medical instrument technical field.

Background

The existing preparation of skin cell suspension is carried out in a laboratory, and the preparation process is as follows: the method comprises the following steps of putting skin tissues to be digested into a centrifuge tube, adding a digestive enzyme solution, putting the skin tissues into a 37 ℃ water bath or an incubator, continuously shaking, filtering after digestion is finished, centrifuging the liquid, discarding supernatant, adding Hanks liquid or serum-free culture solution, carrying out centrifugal rinsing for 1-2 times, removing the supernatant, adding the culture solution to prepare cell suspension, and being quite complex in operation process, needing special personnel and equipment to complete the operation and extremely inconvenient to use clinically.

SUMMERY OF THE UTILITY MODEL

To the above outstanding problem, the utility model provides a preparation ware of skin cell suspension, the utility model discloses a device simple structure can let clinician can prepare skin cell suspension fast, high-efficiently in the operating room, directly is used for the treatment of skin defect diseases such as burn, vitiligo, does not need complicated laboratory staff and equipment, and operation process is simple, quick, microbial contamination's such as greatly reduced bacterium risk.

In order to achieve the purpose, the utility model adopts the following technical proposal:

the utility model discloses a preparation ware of skin cell suspension of first aspect, including following part:

the device comprises a preparation device shell and an operation table arranged on the preparation device shell, wherein a digestion tank 7, a washing tank 8 and a suspension preparation tank 9 are arranged on the operation table, the digestion tank 7 is used for digesting skin tissues, the washing tank 8 is used for washing the digested skin tissues, and the suspension preparation tank 9 is used for preparing skin cell suspension;

the circuit control assembly and the heating assembly are arranged in the preparation shell, the circuit control assembly is used for controlling the heating temperature and time of the digestion tank 7, and the heating assembly is used for heating the digestion tank 7;

the first filter and the second filter are respectively detachably arranged in the digestion tank 7 and the suspension preparation tank 9 and respectively comprise an outer sleeve 5 and an inner core cylinder 3, the outer sleeve 5 is sleeved outside the inner core cylinder 3 in a sleeving manner, a first end of the outer sleeve 5 is provided with a filter screen 1, and a second end of the outer sleeve 5 is provided with a first clamping part 2; the first end of the inner core cylinder 3 is provided with a piston, the second end of the inner core cylinder is provided with a second clamping part 4, and the piston can slide on the inner wall of the outer sleeve 5.

The preparation device, preferably, the sieve mesh diameter of the filter screen 1 in the first filter is less than 10 microns.

The preparation device, preferably, the sieve mesh diameter of the sieve net 1 in the second filter is 50-100 micrometers.

The preparation device preferably further comprises a grinding rod 6, and the grinding rod 6 is used for grinding the digested and washed skin tissue.

The preparation device is preferably provided with a storage bin 10 on the operating platform for accommodating articles required in the operation process.

The preparation device is preferably provided with a heating button 11 on the operation table for controlling the heating of the digestion tank 7.

The preparation device, preferably, the display 12 can monitor the heating temperature to be maintained at 37 ℃ and keep the temperature constant, so that the activity of the digestive enzyme can be maintained at an optimal level.

The preparation device is preferably further provided with a timing button 13 on the operation table, and the timing button is used for controlling the digestion time of the digestion tank 7. The digestion time is convenient to know, and over short digestion time and over long digestion time which can influence the function and the integrity of the cells are avoided.

The preparation device is preferably provided with an indicator light 14 on the operation table, and the indicator light is used for controlling the heating condition of the digestion tank 7.

The preparation device, preferably, the operation panel is further provided with a display 12 for displaying the heating temperature and the heating time.

The second aspect of the present invention provides a method for preparing a suspension of skin cells, comprising the steps of:

(1) placing the skin tissue in the first filter, placing the first filter in the digestion tank 7, and heating and digesting to obtain digested skin tissue;

(2) placing the digested skin tissue in the washing pool 8 for washing to obtain separated skin tissue cells;

(3) and placing the separated skin tissue cells in the suspension preparation pool 9, adding a buffer solution, sucking, extruding and filtering to obtain the skin cell suspension.

Preferably, the preparation method in step 1 comprises the following specific steps: and uniformly mixing sterile water and digestive enzyme, injecting the mixture into the first filter, then placing the first filter into the digestion tank 7, heating, adding skin tissues into the first filter when the temperature reaches 37 ℃, and heating for a period of time to obtain digested skin tissues.

In the preparation method, preferably, the digestive enzyme in the step 1 is trypsin, and the diameter of the screen of the filter screen 1 in the first filter is less than 10 microns.

Preferably, the preparation method in step 2 comprises the following specific steps: and (3) taking the first filter containing the digested skin tissue in the step (1) out of the digestion tank (7), placing the first filter in the washing tank (8), repeatedly sucking, containing enzyme stopping washing liquid in the washing tank (8), wherein the enzyme stopping washing liquid is used for stopping a digestion reaction and separating skin tissue cells to finally obtain separated skin tissue cells.

Preferably, the preparation method in step 3 comprises the following specific steps: pouring the skin tissue cells separated in the step 2 into the second filter in the suspension preparation pool 9, adding a buffer solution, repeatedly sucking and extruding, and filtering to obtain a skin cell suspension.

In the preparation method, preferably, the buffer solution in the step 3 is PBS (phosphate buffer saline) buffer solution, and the diameter of the screen of the filter 1 in the second filter is 50 to 100 micrometers.

Preferably, the preparation method in step 3 further comprises the following specific steps: the remaining skin tissue in the second filter is rolled with a grinding bar 6 until the desired suspension volume is reached.

The utility model discloses owing to take above technical scheme, it has following advantage:

1. the diameter of the sieve pore of the filter screen in the first filter is far larger than that of trypsin, and is also far smaller than that of skin cells, so that the trypsin can prevent the loss of separated cells and effectively discharge the trypsin in the digestion and termination processes;

2. the utility model discloses a skin cell suspension preparation method, it makes cell suspension to get the cell of small piece skin after enzyme digestion, then directly sprays or paints the burn or vitiligo pathological change position that the grinding is good, and operation process is simple, the cell does not need the external culture, need the time short (extract skin, enzyme digestion, cell separation make processes such as suspension about 30 minutes), coverage area is big (according to needs, the suspension that 1 square centimeter skin made can cover 40 to 80 square centimeter pathological change regions), and clinical significance;

3. the utility model discloses the device lets clinician can prepare skin cell suspension fast, high-efficiently in the operating room, directly is used for the treatment of skin defect diseases such as burn, vitiligo, does not need complicated laboratory personnel and equipment, and operation process is simple, quick, microbial contamination's such as greatly reduced bacterium risk.

Drawings

FIG. 1 is a schematic view of the overall structure of the device of the present invention;

FIG. 2 is a schematic view of the structure of a grinding rod in the apparatus of the present invention;

FIG. 3 is a top view of the apparatus of the present invention;

the respective symbols in the figure are as follows:

1-filtering with a filter screen; 2-a first clamping part; 3-inner core cylinder; 4-a second clamping part; 5-an outer sleeve; 6-grinding rod; 7-a digestion tank; 8-a washing tank; 9-a suspension preparation pool; 10-a storage bin, 11-a heating button, 12-a display, 13-a timing button and 14-an indicator light.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention are clearly and completely described below, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by the ordinary person in the art without creative work belong to the protection scope of the present invention.

Example 1

As shown in fig. 1-3, this embodiment provides a device for preparing a skin cell suspension, comprising the following components:

the device comprises a preparation device shell and an operation table arranged on the preparation device shell, wherein the operation table is provided with a digestion tank 7, a washing tank 8, a suspension preparation tank 9 and a display 12, the digestion tank 7 is used for digesting skin tissues, the washing tank 8 is used for washing the digested skin tissues, and the suspension preparation tank 9 is used for preparing skin cell suspensions;

In this embodiment, preferably, the screen mesh diameter of the screen 1 located in the first filter is 8 micrometers.

In this embodiment, preferably, the sieve mesh diameter of the sieve net 1 located in the second filter is 90 micrometers.

In this embodiment, it is preferable that a grinding rod 6 is further included, and the grinding rod 6 is used for grinding the skin tissue after digestion and washing.

In this embodiment, preferably, the operation table is further provided with a storage bin 10 for accommodating articles required in the operation process.

In this embodiment, preferably, a heating button 11 is further disposed on the operation table for controlling the heating of the digestion tank 7.

In this embodiment, preferably, a timing button 13 is further disposed on the operation table for controlling the digestion time of the digestion tank 7.

In this embodiment, preferably, an indicator light 14 is further disposed on the operation table for controlling the heating condition of the digestion tank 7.

Example 2

This example provides a method for preparing a skin cell suspension, comprising the following steps:

(1) injecting 10ml of sterile water into a bottle containing 2.5mg of trypsin, dissolving and uniformly mixing the sterile water, injecting the mixture into the first filter of the digestion tank 7, pressing the heating button 11, adding a small skin tissue block (the skin tissue with the thickness of 0.1-0.2mm, washing with normal saline and cutting into the size of 2-5 mm) after a display lamp turns green when the temperature reaches 37 ℃, pressing the timing button 13 for timing, and obtaining the digested skin tissue after the skin tissue is fluffy and flocculent (about 25 minutes);

(2) taking out the first filter in the digestion tank 7, discharging the liquid in the first filter, repeatedly pumping the enzyme in the washing tank 8 to stop the washing liquid, stopping the enzyme digestion reaction, separating the tissue cells, sucking the first filter, and discharging the liquid to obtain separated skin cells;

(3) the contents (separated skin tissue cells) are poured into the second filter of the suspension preparation tank 9, a little cell suspension preparation solution (PBS buffer solution) is added and repeatedly sucked and pressed, the separated cells are filtered, and the remaining skin tissue in the second filter can be repeatedly rolled and washed by the grinding rod 6 until the volume of the suspension to be prepared is reached.

In the step (1), the small pieces of skin tissues are placed into the first filter for digestion, and the digestive enzyme and the skin pieces to be digested can be fully mixed by repeatedly pumping for several times in the digestion process, so that the separation of cells is facilitated.

In the step (2), the residual digestive enzymes are stopped and/or washed more sufficiently by repeated pumping during the washing process.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects.

Claims

1. A device for preparing a suspension of skin cells, comprising:

the device comprises a preparation device shell and an operation table arranged on the preparation device shell, wherein a digestion tank (7), a washing tank (8) and a suspension preparation tank (9) are arranged on the operation table, the digestion tank (7) is used for digesting skin tissues, the washing tank (8) is used for washing the digested skin tissues, and the suspension preparation tank (9) is used for preparing skin cell suspension;

the circuit control assembly and the heating assembly are arranged in the preparation shell, the circuit control assembly is used for controlling the heating temperature and time of the digestion tank (7), and the heating assembly is used for heating the digestion tank (7);

the first filter and the second filter are respectively detachably arranged in the digestion tank (7) and the suspension preparation tank (9), and both comprise an outer sleeve (5) and an inner core cylinder (3), the outer sleeve (5) is sleeved outside the inner core cylinder (3), the first end of the outer sleeve (5) is provided with a filter screen (1), and the second end of the outer sleeve is provided with a first clamping part (2); the first end of the inner core cylinder (3) is provided with a piston, the second end of the inner core cylinder is provided with a second clamping part (4), and the piston can slide on the inner wall of the outer sleeve (5).

2. The preparation device according to claim 1, wherein the sieve mesh diameter of the sieve (1) located in the first filter is less than 10 microns and the sieve mesh diameter of the sieve (1) located in the second filter is 50-100 microns.

3. The preparation device according to claim 1, further comprising a grinding rod (6), the grinding rod (6) being used for grinding the digested and washed skin tissue.

4. The preparation device according to claim 2, wherein a storage compartment (10) is further provided on the operating table for accommodating articles required during operation.