CN116349877A - Preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions - Google Patents
Preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions Download PDFInfo
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- CN116349877A CN116349877A CN202310394137.9A CN202310394137A CN116349877A CN 116349877 A CN116349877 A CN 116349877A CN 202310394137 A CN202310394137 A CN 202310394137A CN 116349877 A CN116349877 A CN 116349877A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23N—MACHINES OR APPARATUS FOR TREATING HARVESTED FRUIT, VEGETABLES OR FLOWER BULBS IN BULK, NOT OTHERWISE PROVIDED FOR; PEELING VEGETABLES OR FRUIT IN BULK; APPARATUS FOR PREPARING ANIMAL FEEDING- STUFFS
- A23N1/00—Machines or apparatus for extracting juice
- A23N1/02—Machines or apparatus for extracting juice combined with disintegrating or cutting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
- B02C18/10—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged above container
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/16—Details
- B02C18/18—Knives; Mountings thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention belongs to the field of biological enzyme preparation, and particularly relates to a preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions, which comprises the following steps: the method comprises the steps of peeling and stoning 15g of cantaloupe, 15g of apples and 23g of aloe, cleaning, placing in a beating machine, immersing 20g of brown rice in warm water, controlling the water temperature at 30 ℃, placing 20g of soft-immersed brown rice in the beating machine, adding 40ml of water to prepare mixed slurry, pouring the mixed slurry into a grinding machine, adding grinding powder to grind, peeling and stoning the cantaloupe, apples and aloe, pulping, and placing the brown rice in the warm water to be soft, so that the brown rice is convenient to be soft quickly, the raw material extraction process is easier, the beating and grinding are also convenient, the mixed slurry is added into the grinding machine, the grinding powder is added into the grinding machine, the cells are crushed quickly, and the enzyme release is convenient.
Description
Technical Field
The invention relates to the field of biological enzyme preparation, in particular to a preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions.
Background
Biological enzymes refer to a biocatalyst produced by living cells in a living organism. Most consist of proteins. Can efficiently catalyze various biochemical reactions under the condition of mild in the organism and promote the metabolism of the organism. Digestion, absorption, respiration, movement and reproduction in life activities are all enzymatic reaction processes. The enzyme is a catalyst for metabolism in human body, and various biochemical reactions can be carried out in human body only if the enzyme exists. These properties of enzymes allow the intracellular complex metabolism of substances to proceed in a orderly manner, thus adapting the metabolism of substances to normal physiological functions, and if the enzyme activity is weakened due to a defect of a certain enzyme or other reasons, the enzyme-catalyzed reaction is abnormal, so that the metabolism of substances is disturbed and even diseases occur, thus the relationship between the enzyme and the medicine is very close.
In the prior art, in the process of processing raw materials, the preparation method of the biological enzyme with the antibacterial and anti-inflammatory functions has the defects that the processing precision of the raw materials is insufficient and single, so that the extracted biological enzyme has low acquisition rate and is easy to cause a great amount of raw material waste.
Disclosure of Invention
The invention aims to provide a preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions, so as to solve the problems in the background technology.
A preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions comprises the following steps:
(1) Pulping: selecting 10-20g of Hami melon, 10-20g of apple, 15-30g of aloe and 10-30g of brown rice for standby, peeling and stoning 10-20g of Hami melon, 10-20g of apple and 15-30g of aloe, cleaning, placing into a beater, soaking 10-30g of brown rice into soft, placing 10-30g of brown rice into the beater, adding 30ml of water to prepare mixed slurry, pouring the mixed slurry into a grinder, adding grinding powder and grinding;
(2) Culturing raw materials: adding one or two of salt, alkali and acetone into the mixed slurry in the step (1), mixing and stirring, and calibrating the pH value of the mixed solution by using a standard buffer solution;
(3) Solution separation: separating residues in the mixed slurry in the mixed solution through a filter, and collecting the rest mixed pure solution;
(4) And (3) filtering: pouring the mixed pure liquid in the step (3) into a centrifuge tube, placing the centrifuge tube into a centrifuge for centrifugal treatment, performing ultrafiltration treatment on the centrifuged solution, and collecting fermentation liquor;
(5) And (3) drying: and (3) placing the fermentation liquor obtained in the step (4) in a drying box for drying treatment to obtain a freeze-dried product containing biological enzymes.
Preferably, the temperature inside the beater is 10-20 ℃, and the rotating speed is 2800-3600r/min.
Preferably, the steps (2), (3) and (4) are performed in a culture chamber, and the temperature in the culture chamber is 5-15 ℃.
Preferably, the pH of the standard buffer in step (2) is 3-6 and the pH of the calibrated mixed slurry is 4-5.
Preferably, the centrifugation conditions of the step (4) of the centrifuge for centrifuging the mixed solution are: the centrifugal temperature is 6-10 ℃, the centrifugal speed is 8600-12000r/min, and the centrifugal time is 6-10 minutes.
Preferably, the temperature of stirring in the step (2) is 10-35 ℃, and the stirring time is 30-50min.
Preferably, the ultrafiltration membrane of the ultrafiltration treatment has a molecular weight cut-off of 50-60kDa and the ultrafiltration temperature is 40-60 ℃.
Preferably, the temperature of the grinding machine is 5-10 ℃ and the rotating speed is 120-180r/min.
Preferably, the beating machine comprises a base, a beating cylinder is fixedly arranged at the top of the base, a refrigerating mechanism is fixedly arranged on the outer wall of the beating cylinder, and a driving mechanism is fixedly arranged on the inner wall of the base.
The top of the driving mechanism is fixedly provided with a driving shaft, the outer wall of the driving shaft is provided with a plurality of blades, the inner wall of the beating cylinder is slidably connected with a top cover, the top cover protrudes downwards to form a bulge, and a cylindrical sleeve for pushing the blades to move is arranged below the bulge.
The top cover is sleeved on the driving shaft and is in sliding connection with the driving shaft, a connecting plate is fixedly arranged at the top of the top cover, the connecting plate is clamped on the outer wall of the beating cylinder, the end part of the connecting plate extends to the outside of the beating cylinder, a mounting groove is formed in the outer wall of the connecting plate, the inner wall of the mounting groove is rotationally connected with a rotating shaft, two ends of the rotating shaft penetrate through the connecting plate and extend to the outer side of the connecting plate, torsion springs are respectively sleeved at two ends of the rotating shaft, two ends of each torsion spring are respectively fixedly connected with the rotating shaft and the connecting plate, a clamping plate is fixedly arranged on the inner wall of the rotating shaft, a toothed plate is embedded on the outer wall of the beating cylinder, and the inner end of the clamping plate is matched with the toothed plate in a clamping mode.
Preferably, two limiting grooves are formed in the outer wall of the driving shaft, the blades located at the bottom are fixedly connected with the driving shaft, the blades located above are respectively sleeved on the outer wall of the driving shaft, the blades located above are respectively fixedly provided with two limiting blocks, and the limiting blocks are respectively and slidably connected with the inner walls of the limiting grooves.
The lower parts of a plurality of blades above are respectively provided with a spring, a plurality of springs are respectively sleeved on the outer wall of the driving shaft, and two ends of each spring are respectively connected with two adjacent blades.
Compared with the prior art, the invention has the advantages that:
1. peeling and stoning are carried out on cantaloupe, apples and aloe, pulping is carried out, meanwhile, brown rice is placed in warm water to be soaked and soft, so that the brown rice is convenient to be fast soft, the raw material extracting process is easier, pulping and grinding are also convenient, mixed slurry is added into a grinding machine, grinding powder is added into the grinding machine, cells can be quickly broken, enzyme release is convenient, and the prepared biological enzyme has the functions of resisting bacteria, balancing, diminishing inflammation and repairing.
2. In the beating machine, according to the capacity of raw materials, through the slip top cap for the top cap is close to the raw materials liquid level, reduces the inside space of beating section of thick bamboo, thereby in the beating process, can avoid the raw materials to splash everywhere, leads to partial raw materials processing insufficient, influences machining efficiency, makes machining precision relatively poor simultaneously.
3. Through setting up a plurality of blades that can stretch out and draw back, can be according to the capacity of raw materials for a plurality of blade compression are in less space, concentrate and carry out the beating, thereby have further improved beating efficiency, have shortened process time.
Drawings
FIG. 1 is a schematic diagram of the whole structure of a beater in the present invention;
FIG. 2 is a schematic view of the internal structure of the beater according to the present invention;
FIG. 3 is a schematic view of the mounting structure of the clamping plate in the invention;
fig. 4 is a schematic view of a partial structure of the beater according to the present invention.
The reference numerals in the figures illustrate: 1. a base; 2. pulping cylinder; 3. a refrigeration mechanism; 4. a driving mechanism; 5. a drive shaft; 6. a top cover; 7. a connecting plate; 8. a toothed plate; 9. a mounting groove; 10. a rotating shaft; 11. a torsion spring; 12. a clamping plate; 13. a blade; 14. a limit groove; 15. a spring; 16. and a limiting block.
Description of the embodiments
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples
A preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions comprises the following steps:
(1) Pulping: selecting 10g of cantaloupe, 10g of apples, 15g of aloe and 10g of brown rice for later use, peeling and stoning 10g of cantaloupe, 10g of apples and 15g of aloe, cleaning, placing in a beater, soaking 10g of brown rice in soft and quick-softening brown rice, placing brown rice in warm water for soaking, controlling the water temperature at 25 ℃, stirring by a stirring rod, taking out the soaked soft brown rice, filtering out powder water, placing 10g of brown rice in the beater, adding 30ml of water to prepare mixed slurry, pouring the mixed slurry into a grinder, adding grinding powder for grinding, and keeping the temperature of the grinder at 5 ℃ and the rotating speed at 120r/min.
(2) Culturing raw materials: adding one or two of salt, alkali and acetone into the mixed slurry in the step (1), mixing and stirring at the temperature of 10 ℃ for 30min, and calibrating the pH value of the mixed solution by using a standard buffer solution, wherein the pH value of the standard buffer solution is 3, and the pH value of the calibrated mixed slurry is 4.
(3) Solution separation: the residue in the mixed slurry in the mixed liquid was separated by a filter, and the remaining mixed pure liquid was collected.
(4) And (3) filtering: pouring the mixed pure liquid in the step (3) into a centrifuge tube, and placing the centrifuge tube into a centrifuge for centrifugal treatment, wherein the centrifugal conditions of the centrifuge for centrifugal treatment of the mixed solution are as follows: centrifuging at 6deg.C at 8600r/min for 6 min, ultrafiltering the centrifuged solution with ultrafiltration membrane having molecular weight cut-off of 50kDa at 40deg.C, and collecting fermentation broth.
(5) And (3) drying: and (3) placing the fermentation liquor obtained in the step (4) in a drying box for drying treatment to obtain a freeze-dried product containing biological enzymes.
And (3) and (4) operating in a culture chamber, wherein the temperature in the culture chamber is 5 ℃.
The mixed slurry is added into a grinder, and grinding powder is added into the grinder, so that cells can be quickly broken, and enzyme release is facilitated.
Examples
A preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions comprises the following steps:
(1) Pulping: 15g of cantaloupe, 15g of apples, 23g of aloe and 20g of brown rice are selected for standby, 15g of cantaloupe, 15g of apples and 23g of aloe are peeled and stoned, the cantaloupe, the apples and the 23g of aloe are placed in a beater after being cleaned, the temperature inside the beater is 15 ℃, the rotating speed is 3200r/min, after the 20g of brown rice is soaked and soft, the brown rice is placed in warm water for soaking, the water temperature is controlled to be 30 ℃, stirring is carried out through a stirring rod, the soaked and soft brown rice is taken out, powder water is filtered, 20g of brown rice is placed in the beater, 40ml of water is added to prepare mixed slurry, the mixed slurry is poured into a grinder, grinding powder is added to grind, and the temperature of the grinder is 7 ℃, and the rotating speed of the grinder is 1500r/min.
(2) Culturing raw materials: adding one or two of salt, alkali and acetone into the mixed slurry in the step (1), mixing and stirring at 22 ℃ for 40min, and calibrating the pH value of the mixed solution by using a standard buffer solution, wherein the pH value of the standard buffer solution is 4.5, and the pH value of the calibrated mixed slurry is 4.5.
(3) Solution separation: the residue in the mixed slurry in the mixed liquid was separated by a filter, and the remaining mixed pure liquid was collected.
(4) And (3) filtering: pouring the mixed pure liquid in the step (3) into a centrifuge tube, and placing the centrifuge tube into a centrifuge for centrifugal treatment, wherein the centrifugal conditions of the centrifuge for centrifugal treatment of the mixed solution are as follows: centrifuging at 8deg.C, centrifuging at 50r/min for 8 min, ultrafiltering at 50deg.C, and collecting fermentation broth.
(5) And (3) drying: and (3) placing the fermentation liquor obtained in the step (4) in a drying box for drying treatment to obtain a freeze-dried product containing biological enzymes.
And (3) and (4) operating in a culture chamber, wherein the temperature in the culture chamber is 10 ℃.
Examples
A preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions comprises the following steps:
(1) Pulping: selecting 20g of cantaloupe, 20g of apples, 30g of aloe and 30g of brown rice for later use, peeling and stoning 20g of cantaloupe, 20g of apples and 30g of aloe, cleaning, placing in a beater, soaking 30g of brown rice in soft and quick-softening brown rice, placing the brown rice in warm water for soaking, controlling the water temperature at 35 ℃, stirring by a stirring rod, taking out the soaked soft brown rice, filtering out powder water, placing 30g of brown rice in the beater, adding 50ml of water to prepare mixed slurry, pouring the mixed slurry into a grinder, adding grinding powder for grinding, and keeping the temperature of the grinder at 10 ℃ and the rotating speed of 180r/min.
(2) Culturing raw materials: adding one or two of salt, alkali and acetone into the mixed slurry in the step (1), mixing and stirring at 35 ℃ for 50min, and calibrating the pH value of the mixed solution by using a standard buffer solution, wherein the pH value of the standard buffer solution is 6, and the pH value of the calibrated mixed slurry is 5.
(3) Solution separation: the residue in the mixed slurry in the mixed liquid was separated by a filter, and the remaining mixed pure liquid was collected.
(4) And (3) filtering: pouring the mixed pure liquid in the step (3) into a centrifuge tube, and placing the centrifuge tube into a centrifuge for centrifugal treatment, wherein the centrifugal conditions of the centrifuge for centrifugal treatment of the mixed solution are as follows: centrifuging at 10deg.C at 12000r/min for 10 min, ultrafiltering the centrifuged solution for 60kDa, and collecting fermentation broth at 60deg.C.
(5) And (3) drying: and (3) placing the fermentation liquor obtained in the step (4) in a drying box for drying treatment to obtain a freeze-dried product containing biological enzymes.
And (3) operating the step (2), the step (3) and the step (4) in a culture room, wherein the temperature in the culture room is 15 ℃.
The biological enzyme in aloe and brown rice has the functions of resisting bacteria, balancing, diminishing inflammation and repairing, and the biological enzyme breaks down insoluble mucopolysaccharide in cell wall into soluble glycopeptide to crack cell wall and dissolve bacteria, and the lysozyme may be combined with negatively charged viral protein to form complex with DNA, RNA, etc to inactivate virus.
As shown in fig. 1, 2 and 3, the beater comprises a base 1, a beating cylinder 2 is fixedly arranged at the top of the base 1, a refrigerating mechanism 3 is fixedly arranged on the outer wall of the beating cylinder 2, and a driving mechanism 4 is fixedly arranged on the inner wall of the base 1.
The top of the driving mechanism 4 is fixedly provided with a driving shaft 5, the outer wall of the driving shaft 5 is provided with a plurality of blades 13, the inner wall of the pulping barrel 2 is slidably connected with a top cover 6, the top cover 6 protrudes downwards to form a bulge, and a cylindrical sleeve for pushing the blades 13 to move is arranged below the bulge.
The top cover 6 is sleeved on the driving shaft 5 and is in sliding connection with the driving shaft 5, a connecting plate 7 is fixedly arranged at the top of the top cover 6, the connecting plate 7 is clamped on the outer wall of the beating cylinder 2, the end part of the connecting plate 7 extends to the outside of the beating cylinder 2, a mounting groove 9 is formed in the outer wall of the connecting plate 7, a rotating shaft 10 is rotatably connected to the inner wall of the mounting groove 9, two ends of the rotating shaft 10 penetrate through the connecting plate 7 and extend to the outer side of the connecting plate 7, torsion springs 11 are respectively sleeved at two ends of the rotating shaft 10, two ends of each torsion spring 11 are respectively connected and fixed with the rotating shaft 10 and the connecting plate 7, a clamping plate 12 is fixedly arranged on the inner wall of the rotating shaft 10, a toothed plate 8 is embedded on the outer wall of the beating cylinder 2, and the inner end of the clamping plate 12 is in clamping fit with the toothed plate 8; in the beating machine, according to the capacity of raw materials, through slip top cap 6 for top cap 6 is close to the raw materials liquid level, reduces the inside space of beating section of thick bamboo 2, thereby in the beating process, can avoid the raw materials to splash everywhere, leads to partial raw materials processing insufficient, influences machining efficiency, makes the machining precision relatively poor simultaneously.
As shown in fig. 2 and fig. 4, two limiting grooves 14 are formed in the outer wall of the driving shaft 5, the blades 13 at the bottom are fixedly connected with the driving shaft 5, the blades 13 at the upper part are respectively sleeved on the outer wall of the driving shaft 5, two limiting blocks 16 are fixedly arranged on the inner walls of the blades 13 at the upper part, and the limiting blocks 16 are respectively connected with the inner walls of the limiting grooves 14 in a sliding manner.
The processed cantaloupe, apples, water, aloe and brown rice are placed in the pulping barrel 2, the clamping plate 12 is separated from the clamping connection of the toothed plate 8 by pressing the clamping plate 12, the top cover 6 is moved to the position close to the liquid level of the raw material solution by downwards sliding the top cover 6, the clamping plate 12 is loosened, and the clamping plate 12 is clamped with the toothed plate 8 again under the action of the torsion spring 11, so that the position of the top cover 6 is fixed;
when the top cover 6 slides downwards, the bottom cylindrical sleeve pushes the top blade 13 to slide downwards along the driving shaft 5, the springs 15 are compressed, and the distance between the blades 13 is reduced, so that the processing is facilitated, and the activity space of raw materials is reduced;
drive the drive shaft 5 through actuating mechanism 4 and rotate for a plurality of blades 13 carry out crushing processing to the raw materials and handle, can cool down the inside thick liquids of beating thick liquids section of thick bamboo 2 through refrigerating mechanism 3, avoid the high temperature.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The preparation method of the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions is characterized by comprising the following steps of:
(1) Pulping: selecting 10-20g of Hami melon, 10-20g of apple, 15-30g of aloe and 10-30g of brown rice for standby, peeling and stoning 10-20g of Hami melon, 10-20g of apple and 15-30g of aloe, cleaning, placing into a beater, soaking 10-30g of brown rice into soft, placing 10-30g of brown rice into the beater, adding 30ml of water to prepare mixed slurry, pouring the mixed slurry into a grinder, adding grinding powder and grinding;
(2) Culturing raw materials: adding one or two of salt, alkali and acetone into the mixed slurry in the step (1), mixing and stirring, and calibrating the pH value of the mixed solution by using a standard buffer solution;
(3) Solution separation: separating residues in the mixed slurry in the mixed solution through a filter, and collecting the rest mixed pure solution;
(4) And (3) filtering: pouring the mixed pure liquid in the step (3) into a centrifuge tube, placing the centrifuge tube into a centrifuge for centrifugal treatment, performing ultrafiltration treatment on the centrifuged solution, and collecting fermentation liquor;
(5) And (3) drying: and (3) placing the fermentation liquor obtained in the step (4) in a drying box for drying treatment to obtain a freeze-dried product containing biological enzymes.
2. The method for preparing the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to claim 1, wherein the temperature in the beater is 10-20 ℃ and the rotating speed is 2800-3600r/min.
3. The method for preparing the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to claim 1, wherein the steps (2), (3) and (4) are operated in a culture room, and the temperature in the culture room is 5-15 ℃.
4. The method for preparing the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to claim 3, wherein the pH value of the standard buffer solution in the step (2) is 3-6, and the pH value of the calibrated mixed slurry is 4-5.
5. The method for preparing the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to claim 4, wherein the centrifugal conditions of the centrifugal machine for the centrifugal treatment of the mixed solution in the step (4) are as follows: the centrifugal temperature is 6-10 ℃, the centrifugal speed is 8600-12000r/min, and the centrifugal time is 6-10 minutes.
6. The method for preparing a biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions according to any one of claims 1 to 5, wherein the stirring temperature in the step (2) is 10 to 35 ℃ and the stirring time is 30 to 50min.
7. The method for preparing a biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions according to any one of claims 1 to 5, wherein the ultrafiltration membrane subjected to ultrafiltration has a molecular weight cut-off of 50 to 60kDa and a temperature of 40 to 60 ℃.
8. The method for preparing the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to any one of claims 1 to 5, wherein the temperature of the grinder is 5 to 10 ℃ and the rotating speed is 120 to 180r/min.
9. The preparation method of the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to any one of claims 1 to 5, wherein the beating machine comprises a base (1), a beating cylinder (2) is fixedly arranged at the top of the base (1), a refrigerating mechanism (3) is fixedly arranged on the outer wall of the beating cylinder (2), and a driving mechanism (4) is fixedly arranged on the inner wall of the base (1);
the top of the driving mechanism (4) is fixedly provided with a driving shaft (5), the outer wall of the driving shaft (5) is provided with a plurality of blades (13), the inner wall of the beating cylinder (2) is connected with a top cover (6) in a sliding manner, the top cover (6) protrudes downwards to form a bulge, and a cylindrical sleeve for pushing the blades (13) to move is arranged below the bulge;
the top cover (6) is sleeved on the driving shaft (5) and is in sliding connection with the driving shaft, a connecting plate (7) is fixedly arranged at the top of the top cover (6), the connecting plate (7) is clamped on the outer wall of the beating cylinder (2), the end part of the connecting plate (7) extends to the outside of the beating cylinder (2), a mounting groove (9) is formed in the outer wall of the connecting plate (7), a rotating shaft (10) is rotatably connected to the inner wall of the mounting groove (9), the utility model discloses a beating machine, including pivot (10) and connecting plate, pivot (10) both ends pass connecting plate (7) and extend to its outside, pivot (10) both ends cover respectively are equipped with torsional spring (11), torsional spring (11) both ends are connected fixedly with pivot (10) and connecting plate (7) respectively, pivot (10) inner wall has set firmly cardboard (12), beating section of thick bamboo (2) outer wall has inlayed pinion rack (8), cardboard (12) inner and pinion rack (8) joint cooperation.
10. The preparation method of the biological enzyme with the antibacterial, balancing, anti-inflammatory and repairing functions according to claim 9, wherein two limiting grooves (14) are formed in the outer wall of the driving shaft (5), the blades (13) at the bottom are fixedly connected with the driving shaft (5), the blades (13) at the upper part are respectively sleeved on the outer wall of the driving shaft (5), two limiting blocks (16) are fixedly arranged on the inner walls of the blades (13) at the upper part, and the limiting blocks (16) are respectively connected with the inner walls of the limiting grooves (14) in a sliding manner;
springs (15) are arranged below the blades (13) above, the springs (15) are respectively sleeved on the outer wall of the driving shaft (5), and two ends of each spring (15) are respectively connected with two adjacent blades (13).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202310394137.9A CN116349877A (en) | 2023-04-13 | 2023-04-13 | Preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions |
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| CN202310394137.9A CN116349877A (en) | 2023-04-13 | 2023-04-13 | Preparation method of biological enzyme with antibacterial, balancing, anti-inflammatory and repairing functions |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117268066A (en) * | 2023-11-16 | 2023-12-22 | 吉林农业科技学院 | Grinding and drying integrated coarse rice powder processing equipment |
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- 2023-04-13 CN CN202310394137.9A patent/CN116349877A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117268066A (en) * | 2023-11-16 | 2023-12-22 | 吉林农业科技学院 | Grinding and drying integrated coarse rice powder processing equipment |
| CN117268066B (en) * | 2023-11-16 | 2024-01-26 | 吉林农业科技学院 | Grinding and drying integrated coarse rice powder processing equipment |
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