CN101502316A - Method for extracting medlar polysaccharide - Google Patents
Method for extracting medlar polysaccharide Download PDFInfo
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- CN101502316A CN101502316A CNA2009100610849A CN200910061084A CN101502316A CN 101502316 A CN101502316 A CN 101502316A CN A2009100610849 A CNA2009100610849 A CN A2009100610849A CN 200910061084 A CN200910061084 A CN 200910061084A CN 101502316 A CN101502316 A CN 101502316A
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Abstract
The invention discloses an extraction method of wolfberry polysaccharide, comprising the following steps: A picking wolfberry; B grinding the fresh wolfberry into homogeneous pulp using a pulp grinder; C pouring the homogeneous pulp into a container; D microwave processing the homogeneous pulp to 50-100 centigrade and boiling the homogeneous pulp for 10-60 minute; E stopping the microwave processing and adding the purified water into the boiled pulp and continuously stirring up the pulp until the temperature is reduced to 50-80 centigrade; F inserting an ultrasonic rod to perform ultrasonic treatment for 20-90 minute; G processing the pulp being subjected to ultrasonic treatment using an industry centrifuges at 3000-5000 r and spilling out the supernatant and decompression concentrating H at 60-90 degree and adding alcohol until the final alcohol concentration is 80-90% and separating and precipitating the mixture using the industry centrifuges at 3000-5000 r to obtain the polysaccharide raw powder or coarse paste. The extraction method has features of high efficiency, energy saving and quick extraction speed. The extraction method solves the problem that the work for drying the wolfberry is heavy and the energy source is waste in Northwest China in harvest season and the extraction technology of the wolfberry polysaccharide is increased and the wolfberry polysaccharide yield is increased.
Description
Technical field
The polysaccharide that the present invention relates to a kind of fresh fruit of Chinese wolfberry extracts microwave and ultrasonic technology field.More specifically relate to the energy-efficient extracting method of a kind of LBP-X, be particularly suitable for the direct fruit of Chinese wolfberry of handling of lycium barbarum harvest season, and do not need to dry or dry, using manpower and material resources sparingly and the energy, rapid extraction technology has also improved the extraction efficiency of manufacturing enterprise simultaneously.
Background technology
Lycium barbarum is the traditional rare traditional Chinese medicine of China.Compendium of Material Medica record: " nourishing liver and kidney, benefiting shrewd head "." book on Chinese herbal medicine converges and says " is described: " matrimony vine can make gas fill, and blood can be mended, Yang Kesheng, and the moon can be grown, rheumatism is dispelled, and perfect magical effect is arranged ".The successive dynasties traditional Chinese medical science think often eat matrimony vine can " hard muscles and bones, old, the cold-resistant heat of making light of one's life by commiting suicide ".So it usually is taken as nourishing and takes good care of and antidotal good medicine.According to the clinical medicine checking, matrimony vine can also be treated chronic renal failure.Concerning the Ms, can improve skin and absorb the ability that oxygen divides, often eat matrimony vine and can improve looks.Its main active is a LBP-X, and polyoses content reaches 5-8%.The purification LBP-X is produced the dietotherapeutic health products and is had boundless market prospects.
One aspect of the present invention is based on urgent problem in the lycium barbarum production practices of NORTHWEST CHINA area: the fruit of Chinese wolfberry of the overcast and rainy harvesting of airing in time, cause large quantities of fruits of Chinese wolfberry to go rotten, illegal retailer carries out sulfur fumigation to the fruit of Chinese wolfberry that goes rotten and puts on market then, has had a strong impact on the quality safety of the fruit of Chinese wolfberry; In order in time to dry the fruit of Chinese wolfberry, a lot of peasants adopt the way of baking, but ten thousand tons of fruit of Chinese wolfberry 20-30 are produced in the NORTHWEST CHINA area at present per year, toast a large amount of coal resources of waste.Therefore the fresh fruit of Chinese wolfberry of plucking is directly extracted for using manpower and material resources sparingly, reduce energy waste and have important role.On the other hand, the enterprise of fruit of Chinese wolfberry polysaccharide extraction at present adopts the fruit of Chinese wolfberry of oven dry to soak extraction polysaccharide or hot water refluxing extraction polysaccharide as raw material, and process time is long or energy resource consumption is big, and input cost is big.
Microwave and ultrasonic wave auxiliary extraction technology are in recent years emerging active components of plants extractive techniques.The pulverizing medicinal materials technology still can not reach the ultramicro grinding effect of cell grade at present, and for the high medicinal material of this sugar content of lycium barbarum, drying material is as easy as rolling off a log caking in conventional crushing process, makes troubles for pulverizing and extraction process.Microwave has good tissue and cytoclasis effect.Microwave can be absorbed by flesh tissue piece or intracellular polar solvent, make cell or tissue piece temperature inside raise rapidly, vaporize, and burst apart, thereby destroyed eucaryotic cell structure, removed cell membrane and cell membrane active component in the born of the same parents has been diffused into inhibition in the solvent.Therefore, microwave is a kind of good tool of ultramicro grinding.Simultaneously can also promote that slurry temperature raises rapidly during microwave action, be the even instrument of heating efficiently in the extraction process.Hyperacoustic ultrasonic cavitation effect and physical shock effect can play good mechanical agitation, promote the active component in the smudge cells to be dissolved into the homogeneous concentration of extracting in the solvent and quickening to form the inside and outside active component of cell, thereby extract intracellular active component most effectively the soonest.Ultrasonic wave extraction element commonly used at present comprises sonde-type extraction element and water-bath type extraction element.The former is suitable for large-scale industrial production, and the latter is mainly used in the pre-treatment of lab analysis sample.This patent adopts the sonde-type extraction element.The Microwave Extraction device has sealed high pressure to extract and normal pressure extracts.Sealed high pressure extracts has the high and uneasy congruent unfavorable factor of equipment cost.The normal pressure extraction element has advantages such as Controllable Temperature, equipment are simple, safety.This patent adopts the normal pressure microwave treatment.Chinese patent CN2748147Y, CN1651896A, CN2773664Y, CN2822737Y, CN2880211Y have announced several ultrasonic wave Microwave Extraction devices.But existing microwave ultrasonic wave extraction element does not relate to the ultramicro grinding function that the active component of fresh biomaterial extracted and utilized microwave.This patent is compared with existing extractive technique has following characteristics: (1) directly utilizes the fresh fruit of Chinese wolfberry to pulverize the extraction polysaccharide, has removed oven dry or airing process from; (2) microwave and ultrasonic wave stepping action, microwave is used for the homogenate tissue is carried out further ultramicro grinding and heating, and ultrasonic wave is used for that abundant material crushed is carried out polysaccharide and extracts.
Summary of the invention
The objective of the invention is to be to provide a kind of extraction method of wolfberry polysaccharide, easy to implement the method, easy and simple to handle, utilize microwave to carry out ultramicro grinding, and extract with ultrasonic wave to extracting material, technological process is simple, and is with low cost, is suitable for large-scale production.
In order to realize above-mentioned task, the present invention adopts following technical measures:
Of the present invention is to be to provide to utilize the direct extractive technique of the fresh fruit of Chinese wolfberry to polysaccharide, dried wolfberry causes in the lycium barbarum production manpower and materials and energy waste have been reduced, solved a great problem in producing, through overtesting relatively, the fresh fruit of Chinese wolfberry directly extracts the yield a little more than extraction polysaccharide from dried wolfberry of polysaccharide.Therefore the art of this patent is worth large-scale promotion.
A kind of fresh fruit of Chinese wolfberry polysaccharide extraction step is as follows:
1, wins the fruit of Chinese wolfberry of abundant maturation;
2, with fiberizer the fresh fruit of Chinese wolfberry is worn into homogenate, according to homogenate fineness defibrination 1-4 time repeatedly;
3, fresh fruit of Chinese wolfberry homogenate is poured in the container, this container bottom is made with glass plate, microwave sees through glass heating using microwave and microwave pulverization is carried out in homogenate from the bottom, with microwave from top or the side heating using microwave and microwave pulverization are carried out in homogenate, the side of container (microwave passes through face) makes with glass material and is convenient to microwave and passes through.
4, microwave treatment is 50-100 degrees centigrade to homogenized temperature, and boiling time continues 10-60 minutes;
5, stop microwave treatment, the slurries the inside adding pure water toward boiling constantly is stirred to temperature and reduces to 50-80 degree centigrade:
6, insert ultrasonic rod, ultrasonic processing 20-90 minute;
7, change the homogenate of handling after ultrasonic with industrial centrifugal machine 3000-5000, pour out supernatant, the decompression of 60-90 degree concentrates
8, add ethanol and regulate the ethanol final concentration, change precipitation separation, obtain polysaccharide meal or thick cream with industrial centrifugal machine 3000-5000 to 80-90%;
The present invention compared with prior art has the following advantages and effect: simple to operate, energy savings; Extraction time is short, the extraction efficiency height.The invention solves the heavy work and the energy waste of NORTHWEST CHINA area harvest season dried wolfberry, also simplified and extracted the technology of fruit of Chinese wolfberry polysaccharide and improved polysaccharide yield.Be suitable for large-scale production.Directly the polyoses content that extracts from fresh fruit is slightly high with the polyoses content that extracts from dry fruit.Adopt a kind of sampling test, the polyoses content that process mensuration is extracted from fresh fruit is more than 6.35%, and the polyoses content that conventional method is extracted from dry fruit (table 1) about 6.24%.Compare with conventional method, this law has been saved extraction time, has been reduced energy resource consumption, recovery rate does not reduce.
Table 1: this patent method is extracted and traditional dry fruit extracts relatively
The specific embodiment
Embodiment 1:
It is as follows that a kind of fresh fruit of Chinese wolfberry extracts the polysaccharide operating procedure:
1, wins the fruit of Chinese wolfberry of abundant maturation;
2, with fiberizer the fresh fruit of Chinese wolfberry is worn into homogenate, according to homogenate fineness defibrination 1 time or 2 times or 3 times or 4 times repeatedly;
3, fresh fruit of Chinese wolfberry homogenate is poured in the container, and this container bottom answers glass plate to make, and microwave sees through glass to the homogenate effect from the bottom; With microwave from top or the side to the homogenate effect, microwaves corresponding is made with glass material by face;
4, microwave treatment to homogenized temperature is 50 degrees centigrade or 60 degrees centigrade or 70 degrees centigrade or 80 degrees centigrade or 90 degrees centigrade or 100 degrees centigrade, and boiling time continues 10 minutes or 20 minutes or 30 minutes or 40 minutes or 50 minutes or 60 minutes 60 minutes;
5, stop microwave treatment, the slurries the inside adding pure water toward boiling constantly is stirred to temperature and reduces to 50 degrees centigrade or 60 degrees centigrade or 70 degrees centigrade or 80 degrees centigrade:
6, insert ultrasonic rod, ultrasonic processing 20 minutes or 30 minutes or 40 minutes or 50 minutes or 60 minutes or 70 minutes or 80 minutes or 90 minutes;
7, change with industrial centrifugal machine 3000 or 4000 change or 5000 change the homogenate of handling after ultrasonic, pour out supernatant, the decompression of 60-90 degree concentrates
8, add ethanol and regulate ethanol final concentration to 80% or 85% or 90%, change or 4000 change or 5000 change precipitation separations, obtain the thick cream of polysaccharide, can load and transport and enter the workshop and be further purified (common process) with industrial centrifugal machine 3000.
Claims (1)
1, a kind of extraction method of wolfberry polysaccharide the steps include:
A, win the fruit of Chinese wolfberry of abundant maturation;
B, the fresh fruit of Chinese wolfberry is worn into homogenate, according to homogenate fineness defibrination 1-4 time repeatedly with fiberizer;
C, fresh fruit of Chinese wolfberry homogenate are poured in the container, and this container bottom answers glass plate to make, and microwave sees through glass to the homogenate effect from the bottom, or with microwave from top or the side to the homogenate effect, microwaves corresponding is made with glass material by face;
D, microwave treatment are 50-100 degrees centigrade to homogenized temperature, and boiling time continues 10-60 minutes;
E, stop microwave treatment, add pure water, constantly be stirred to temperature and reduce to 50-80 degree centigrade toward the slurries the inside of boiling:
F, insert ultrasonic rod, ultrasonic processing 20-90 minute;
G, change the homogenate of handling after ultrasonic with industrial centrifugal machine 3000-5000, pour out supernatant, the decompression of 60-90 degree concentrates;
H, adding ethanol are regulated the ethanol final concentration to 80-90%, change precipitation separation with industrial centrifugal machine 3000-5000, obtain the polysaccharide powder.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100610849A CN101502316A (en) | 2009-03-11 | 2009-03-11 | Method for extracting medlar polysaccharide |
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| CNA2009100610849A CN101502316A (en) | 2009-03-11 | 2009-03-11 | Method for extracting medlar polysaccharide |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211149A (en) * | 2013-04-22 | 2013-07-24 | 重庆阿尔康生物工程有限公司 | Perilla composite capsule and preparation method thereof |
| CN103549421A (en) * | 2013-10-24 | 2014-02-05 | 格尔木源鑫堂生物科技有限公司 | Multidimensional quick-acting medlar powder and production method thereof |
| CN103637268A (en) * | 2013-12-25 | 2014-03-19 | 山东阿如拉药物研究开发有限公司 | Instant medlar sea sedge with function of improving memory |
| CN104833560A (en) * | 2015-04-27 | 2015-08-12 | 宁夏森淼种业生物工程有限公司 | Quickly-shearing nondestructive method for extracting mineral elements from wolfberry leaf |
| CN104906259A (en) * | 2015-06-19 | 2015-09-16 | 湖北中烟工业有限责任公司 | Preparation method of wolfberry extract |
| CN113633657A (en) * | 2021-05-08 | 2021-11-12 | 中国人民解放军海军军医大学 | Pharmaceutical composition for improving anti-fatigue, anti-hypoxia, heat-resistant and cold-resistant abilities of organism and application thereof |
| CN114652633A (en) * | 2022-05-20 | 2022-06-24 | 广州优理氏生物科技有限公司 | Preparation method of polypeptide enzymolysis and fermentation composition and application of polypeptide enzymolysis and fermentation composition in skin care products |
| CN115040471A (en) * | 2022-02-17 | 2022-09-13 | 张志昂 | Product containing lycium barbarum polysaccharide for nourishing retinal nerves and preparation method thereof |
-
2009
- 2009-03-11 CN CNA2009100610849A patent/CN101502316A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103211149A (en) * | 2013-04-22 | 2013-07-24 | 重庆阿尔康生物工程有限公司 | Perilla composite capsule and preparation method thereof |
| CN103549421A (en) * | 2013-10-24 | 2014-02-05 | 格尔木源鑫堂生物科技有限公司 | Multidimensional quick-acting medlar powder and production method thereof |
| CN103637268A (en) * | 2013-12-25 | 2014-03-19 | 山东阿如拉药物研究开发有限公司 | Instant medlar sea sedge with function of improving memory |
| CN103637268B (en) * | 2013-12-25 | 2015-09-09 | 山东金诃药物研究开发有限公司 | A kind of instant medlar sea sedge with Improving memory function |
| CN104833560A (en) * | 2015-04-27 | 2015-08-12 | 宁夏森淼种业生物工程有限公司 | Quickly-shearing nondestructive method for extracting mineral elements from wolfberry leaf |
| CN104833560B (en) * | 2015-04-27 | 2017-09-19 | 宁夏森淼种业生物工程有限公司 | A kind of method that flash shearing non-destructive extracts folium lycii mineral element |
| CN104906259A (en) * | 2015-06-19 | 2015-09-16 | 湖北中烟工业有限责任公司 | Preparation method of wolfberry extract |
| CN113633657A (en) * | 2021-05-08 | 2021-11-12 | 中国人民解放军海军军医大学 | Pharmaceutical composition for improving anti-fatigue, anti-hypoxia, heat-resistant and cold-resistant abilities of organism and application thereof |
| CN113633657B (en) * | 2021-05-08 | 2024-03-01 | 中国人民解放军海军军医大学 | Pharmaceutical composition for improving body anti-fatigue, hypoxia-resistant, heat-resistant and cold-resistant capacities and application thereof |
| CN115040471A (en) * | 2022-02-17 | 2022-09-13 | 张志昂 | Product containing lycium barbarum polysaccharide for nourishing retinal nerves and preparation method thereof |
| CN114652633A (en) * | 2022-05-20 | 2022-06-24 | 广州优理氏生物科技有限公司 | Preparation method of polypeptide enzymolysis and fermentation composition and application of polypeptide enzymolysis and fermentation composition in skin care products |
| CN114652633B (en) * | 2022-05-20 | 2022-08-02 | 广州优理氏生物科技有限公司 | Preparation method of polypeptide enzymolysis and fermentation composition and application of polypeptide enzymolysis and fermentation composition in skin care products |
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Open date: 20090812 |