CN102102080B - Automatic biological component extracting device - Google Patents
Automatic biological component extracting device Download PDFInfo
- Publication number
- CN102102080B CN102102080B CN 201010564824 CN201010564824A CN102102080B CN 102102080 B CN102102080 B CN 102102080B CN 201010564824 CN201010564824 CN 201010564824 CN 201010564824 A CN201010564824 A CN 201010564824A CN 102102080 B CN102102080 B CN 102102080B
- Authority
- CN
- China
- Prior art keywords
- centrifugal
- cam
- suspension member
- slide block
- cam follower
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention relates to the technical field of biological sample treatment automation, in particular to an automatic biological component extracting device. The device comprises a cam mechanism, a cam lifting mechanism and a centrifugal mechanism, wherein the centrifugal mechanism has a horizontal rotor structure; a series of centrifugal suspension members are uniformly arranged around an axis of a centrifugal rotor body; the centrifugal suspension members can freely rotate vertically and horizontally around a respective axis; a slideway is processed on each centrifugal suspension member and is provided with a slide block; and a certain number of biological samples can be arranged in an operation bottle, and one or more grinding colliders are arranged in the operation bottle. The whole cam mechanism can move up and down under the driving of a cam lifting mechanism, and at the highest point, a cam push rod and the slide block are connected with each other, and the slide block can reciprocate along the slideway under the driving of the cam; and at the lowest point, the cam push rod is separated from the slide block. The device is compact in structure, and can be widely applied to automatic operation of extracting biological components such as nucleic acids and proteins from the biological samples such as animals, plants and microorganisms.
Description
Technical field
The present invention relates to biological specimen and handle technical field of automation, relate in particular to the device that a kind of biological components automatization is extracted.
Background technology
In biochemical research and clinical application field, extracting particular organisms components such as nucleic acid, protein from biological specimens such as animal tissues, plant tissue, microorganism is to use one of general routine techniques, also is the basis of follow-up study and experiment.The related operation of this technology comprises that the grinding of solid-state sample, broken born of the same parents, solid-liquid mix, centrifugal, filtration, chromatography, electrophoresis etc.According to the difference of targeted biological component to be extracted and primeval life sample, by the various combination to above operation, can realize the extraction to specific biological components or allied substances.Wherein, the extracting method of a kind of " grinding-mixed dissolution-centrifugal " is owing to process simply is widely used, and its concrete operations flow process is: 1. with the biological specimen grind into powder; 2. add specific extraction liquid in powder, vibration makes powder fully mix with extracting solution, and biological components to be extracted dissolves in extracting solution; 3. mixed solution is carried out centrifugal, solid matter be deposited to the bottom; 4. extract supernatant, obtain the particular organisms component.
Involved concrete operations comprise grinding, solid-liquid mixing and centrifugal in the said extracted method.At per step operation, all have matured product in the market.Find by the investigation to existing patent: nearly all relevant patent all is the improvement of doing at a certain concrete operations, lacks the innovation that covers whole process.For example, patent " detoxifcation burn cream super micro crushing of Chinese medicinal material device " (CN020092007559) has proposed a kind of ball milled that utilizes and has ground, and utilizes screen cloth to filter, to reduce the device of gained powder mean particle size; Patent " continous way solid-liquid separating centrifuge " (CN200720006770) has been mentioned a kind of centrifugal device based on rotary drum (cone-element); Patent " the blood plasma list is adopted centrifuge separator " (CN00248423) has been mentioned a kind of for to the blood process, to the centrifugal separating device of blood plasma on-site collection; Patent " with the device of blood plasma in the centrifugal separation separating whole blood " (CN 91105326) has proposed a kind of separation of whole blood device that can collect blood plasma automatically.
Use the instrument that separates to carry out the extraction of biological components, need operator to participate in the operation of whole process from start to finish, thereby waste a large amount of energy and times.Particularly solidify for those operating processes, and the constantly operation of increase of extraction scale, this unnecessary waste seems more outstanding.Therefore, the present invention proposes a kind of biological components automatization extraction element that can finish above-mentioned every link continuously, to address the above problem.
Summary of the invention
The biological components automatization extraction element that the purpose of this invention is to provide a kind of can finishing continuously " grinding-mixed dissolution-centrifugal " operation.
For achieving the above object, the present invention adopts following technical scheme.
The structure of this device comprises: central shaft, be set in cylindrical cam, cam follower on the central shaft, be arranged in the cam lifting mechanism of cylindrical cam below, the centrifugal rotor body that is positioned at the central shaft top, the grinding that is suspended on centrifugal suspension member, the operation bottle on the centrifugal rotor body and is contained in the operation bottle hit son.Described centrifugal rotor body adopts the horizontal rotor structure, on the centrifugal rotor body around self axis a series of centrifugal suspension members of evenly arranging; Centrifugal suspension member can freely rotate between vertical and level attitude around self axis; Be processed with slideway on the centrifugal suspension member, a slide block is installed in the slideway, the operation bottle is fixed on the slide block; Cylindrical cam overlaps with the centrifugal rotor body axis, and cam follower equates with centrifugal suspension member quantity, and evenly arranges around the axis of cylindrical cam; Described cam follower rolls with cylindrical cam and contacts, and cam follower pumps under the driving of cylindrical cam and cam lifting mechanism.
The low temperature module is installed above described cylindrical cam, and described low temperature module moves up and down under the driving of cam lifting mechanism with cylindrical cam; Described low temperature module upper edge circumference a series of and the cam follower hole one to one that evenly distributes, when cam follower during at vertex, each centrifugal suspension member inserts corresponding hole, and slide block and cam follower interconnect, and realize the cryogenic freezing grinding; When cam follower during in lower-most point, each centrifugal suspension member and hole break away from fully, and its motion is not interfered by hole.
Described low temperature module is made of cryosphere and thermal insulation layer, and cryosphere contacts with centrifugal suspension member, and thermal insulation layer parcel cryosphere prevents that low temperature module surface from producing the globule because of condensation.
Described low temperature module adopts liquid nitrogen cooling to realize low temperature, adopts vacuum insulating to realize thermal isolation.
Described cylindrical cam adopts the shape enclosed construction, and namely cam follower keeps in touch with cylindrical cam in whole motion process all the time.
Interconnecting by magnetic force between described cam follower and the described slide block realizes.
Described cam lifting mechanism adopts screw mechanism, by the rotation of screw rod, drives cylindrical cam and attached component and moves up and down.
Described centrifugal rotor body is disc structure, and is hanging centrifugal suspension member place processing troug hing, and during high speed centrifugation, centrifugal suspension member is subjected to centrifugal action to be positioned at groove on the centrifugal rotor body, to reduce windage to centrifugal influence.
Beneficial effect of the present invention is:
This device can be finished " grinding-mixed dissolution-centrifugal " operation continuously to biological specimen, compact construction, moving parts is few, easy to operate, improved working efficiency, can be widely used in from biological specimens such as animal, plant and microorganism, extracting the automated operation of particular organisms components such as nucleic acid, protein, overcome the deficiencies in the prior art.
Description of drawings
Fig. 1 is the biological components automatization extraction element structural representation of one embodiment of the present invention, and cam is in lower-most point at this moment, and cam follower and slide block break away from, and centrifugal rotor body and cam all remain static.
Fig. 2 is the view of this device when carrying out the operation of grinding or mixed dissolution.At this moment, cam is at vertex, and cam follower and slide block interconnect, and it is static that the centrifugal rotor body keeps, the cam rotation, and driving operation bottle is done with slide block and is pumped.
Fig. 3 is the view when carrying out centrifugally operated.At this moment, cam is in lower-most point, and cam follower and slide block break away from, and it is static that cam keeps, and the centrifugal rotor body carries centrifugal suspension member high speed rotating.
Fig. 4 is the diagrammatic cross-section of centrifugal suspension member part;
Fig. 5 is the diagrammatic cross-section of low temperature module;
Fig. 6 is for adopting the cam lifting device structure synoptic diagram of screw mechanism.
Number in the figure:
1-centrifugal rotor body; The centrifugal suspension member of 2-; 3-low temperature module; The 4-cam follower; The 5-cylindrical cam; The 6-cam lifting mechanism; The 7-slide block; 8-operates bottle; 9-grinds and hits son; 10-operates bottle cap; The 11-slideway; The fastening trim ring of 12-; The 13-vacuum layer; 14-liquid nitrogen sleeve; 15-liquid nitrogen layer; 16-push rod orienting lug; The 17-fixed muffle; 18-liquid nitrogen conduit; The 19-sealed shell; 20-centrifugal shaft pedestal; The 21-thrust block; The 22-lifting screw; The 23-lifting nut; The 24-roller; The 25-permanent magnet; The 26-lifting guide pillar; The 27-radial bearing; The 28-central shaft.
Embodiment
The invention provides a kind of biological components automatization extraction element, the present invention will be further described below in conjunction with drawings and Examples.
As Fig. 1-shown in Figure 6, the structure of this device comprises: central shaft 28, be set in cylindrical cam 5, cam follower 4 on the central shaft 28, be arranged in the cam lifting mechanism 6 of cylindrical cam 5 belows, the centrifugal rotor body 1 that is positioned at central shaft 28 tops, the grinding that is suspended on centrifugal suspension member 2, the operation bottle 8 on the centrifugal rotor body 1 and is contained in operation bottle 8 hit son 9.
Centrifugal rotor body 1 adopts the horizontal rotor structure, on centrifugal rotor body 1 around axis 4 the centrifugal suspension members 2 of evenly arranging.The agent structure of centrifugal rotor body 1 is a rotational symmetry disk, has respective slot in centrifugal suspension member 2 suspensions place.Centrifugal suspension member 2 can freely rotate between vertical and level attitude around self axis.When it is in horizontality, can be positioned at groove fully.Be processed with slideway 11 on the centrifugal suspension member 2, a slide block 7 is installed in the slideway 11, slide block 7 can move reciprocatingly along slideway 11 in certain distance, its material selection ferromagnetic substance.Operation bottle 8 and operation bottle cap 10 be by being threaded, its internal cavities biological specimen of some amount of can packing into, and one or several grindings are housed simultaneously hit sub 9.Operation bottle 8 can be fixed on the slide block 7 by fastening trim ring 12, and plays motion with one.
Cylindrical cam 5 sides are processed with groove, and cam follower 4 terminal rollers 24 insert groove, its top mounting permanent magnet 25.When cam rotates, can drive push rod and move up and down.Cylindrical cam 5 and upper groove thereof are axially symmetric structure, and namely the characteristics of motion and the characteristics of motion 180～360 ° scope in of cam follower 4 in 0～180 ° of scope is identical.
On the low temperature module 3 around axis 4 fixed muffles 17 of evenly arranging, axis equated to the distance of centrifugal rotor body 1 axis when fixed muffle 17 axis vertically hung with centrifugal suspension member 2 to low temperature module 3 axial line distance, and its internal surface can fit tightly with centrifugal suspension member 2 outside surfaces.The fixed muffle 17 outer liquid nitrogen sleeves 14 of installing, cavity constitutes liquid nitrogen layer 15 between the two; Cavity constitutes vacuum layer 13 between liquid nitrogen sleeve 14 and the sealed shell 19.Liquid nitrogen conduit 18 passes vacuum layer 13, is communicated with liquid nitrogen layer 15.Fixed muffle 17 bottoms are equipped with push rod orienting lug 16, to prevent that cam follower 4 from self taking place rotate when moving up and down.
Cylindrical cam 5 and low temperature module 3 are installed on the lifting nut 23, and wherein cylindrical cam 5 is supported by radial bearing 27, can be around lifting nut 23 rotations.Lifting screw 22 is supported by thrust block 21, can be around 20 rotations of centrifugal shaft pedestal.Lifting screw 22 constitutes screw-pair with lifting nut 23, and when lifting screw 22 rotations, lifting nut 23 can be moved up and down by lifting guide pillar 26 guiding.When lifting nut 23 was positioned at vertex, centrifugal suspension member 2 inserted fixed muffle 17, and fits tightly.The permanent magnet 25 at cam follower 4 tops relies on magnetic force to be connected with slide block 7.During the cam rotation, can drive slide block 7 and pump.When lifting nut 23 was positioned at lower-most point, centrifugal suspension member 2 was extracted out from fixed muffle 17, and slide block 7 breaks away from cam follower 4 and is connected.During 1 rotation of centrifugal rotor body, centrifugal suspension member 2 is subjected to centrifugal action to be in horizontality.
When using this device to carry out the biological components automatization to extract operation, at first, with a certain amount of biological specimen operation bottle 8 of packing into, put into one or several grindings simultaneously and hit son 9, screw operation bottle cap 10.To operate bottle and 8 put into centrifugal suspension member 2 inner slides 7, and fixing with fastening trim ring 12, making itself and slide block 7 fixed is one.
Air in the vacuum layer in the low temperature module 3 13 is extracted out, formed vacuum environment.Utilize analogous instrument such as liquid nitrogen pump by liquid nitrogen conduit 18, make all or part of liquid nitrogen that are full of in the liquid nitrogen layer 15.By heat passage, at the inner low temperature environment that forms of fixing guide.The existence of vacuum layer 13 can avoid low temperature module 3 surfaces because of the condensation attached water.
The stop solution nitrogen supply, treat that liquid nitrogen vaporization in the liquid nitrogen layer 15 is intact after, in liquid nitrogen layer 15, feed dry air by liquid nitrogen conduit 18, make that biological specimen powder heat up in the operation bottle 8.Opening operation bottle cap 10 is to operating the extracting solution that adds some amount in the bottle 8.Closed procedure bottle cap 10, cylindrical cam 5 low speed rotation.Under the effect of mass force, grinding is hit sub 9 powder and the extracting solutions that will operate bottle 8 and is fully mixed.Biological components to be extracted is dissolved in extracting solution.
Behind the centrifugal certain hour, centrifugal rotor body 1 stops operating, and will operate bottle 8 and take off from slide block 7, and opening operation bottle cap 10 is drawn supernatant, obtains being dissolved with the solution of particular organisms component.
Above embodiment only is used for explanation the present invention; and be not limitation of the present invention; the those of ordinary skill in relevant technologies field; under the situation that does not break away from the spirit and scope of the present invention; can also make a variety of changes and modification; therefore all technical schemes that are equal to also belong to category of the present invention, and scope of patent protection of the present invention should be defined by the claims.
Claims (6)
1. biological components automatization extraction element, comprise: central shaft (28), be set in cylindrical cam (5), cam follower (4) on the central shaft (28), be arranged in the cam lifting mechanism (6) of cylindrical cam (5) below, the centrifugal rotor body (1) that is positioned at central shaft (28) top, the grinding that is suspended on centrifugal suspension member (2), the operation bottle (8) on the centrifugal rotor body (1) and is contained in operation bottle (8) hit son (9), it is characterized in that
Described centrifugal rotor body (1) adopts the horizontal rotor structure, goes up around self axis a series of centrifugal suspension members (2) of evenly arranging at centrifugal rotor body (1); Centrifugal suspension member (2) can freely rotate between vertical and level attitude around self axis; Be processed with slideway (11) on the centrifugal suspension member (2), a slide block (7) is installed in the slideway (11), operation bottle (8) is fixed on the slide block (7); Cylindrical cam (5) and centrifugal rotor body (1) dead in line, cam follower (4) equates with centrifugal suspension member (2) quantity, and evenly arranges around the axis of cylindrical cam (5); Described cam follower (4) rolls with cylindrical cam (5) and contacts, and cam follower (4) pumps under the driving of cylindrical cam (5) and cam lifting mechanism (6);
Described centrifugal rotor body (1) is disc structure, and is hanging centrifugal suspension member place processing troug hing, and during high speed centrifugation, centrifugal suspension member (2) is subjected to centrifugal action to be positioned at centrifugal rotor body (1) and goes up groove, to reduce windage to centrifugal influence;
Low temperature module (3) is installed in top at described cylindrical cam (5), and described low temperature module (3) moves up and down under the driving of cam lifting mechanism (6) with cylindrical cam (5); Described low temperature module (3) upper edge circumference a series of and cam follower (4) hole one to one that evenly distributes, when cam follower (4) during at vertex, each centrifugal suspension member (2) inserts corresponding hole, and slide block (7) interconnects with cam follower (4), realizes the cryogenic freezing grinding; When cam follower (4) during in lower-most point, each centrifugal suspension member (2) breaks away from fully with hole, and its motion is not interfered by hole.
2. biological components automatization extraction element according to claim 1, it is characterized in that described low temperature module (3) is made of cryosphere and thermal insulation layer, cryosphere contacts with centrifugal suspension member (2), thermal insulation layer parcel cryosphere prevents that low temperature module surface from producing the globule because of condensation.
3. biological components automatization extraction element according to claim 1 is characterized in that, described low temperature module (3) adopts liquid nitrogen cooling to realize low temperature, adopts vacuum insulating to realize thermal isolation.
4. biological components automatization extraction element according to claim 1 is characterized in that, described cam follower (4) keeps in touch with cylindrical cam (5) in whole motion process all the time.
5. biological components automatization extraction element according to claim 1 is characterized in that, interconnecting by magnetic force between described cam follower (4) and the described slide block realizes.
6. biological components automatization extraction element according to claim 1 is characterized in that, described cam lifting mechanism (6) adopts screw mechanism, by the rotation of screw rod, drives cylindrical cam and attached component and moves up and down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564824 CN102102080B (en) | 2010-11-24 | 2010-11-24 | Automatic biological component extracting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010564824 CN102102080B (en) | 2010-11-24 | 2010-11-24 | Automatic biological component extracting device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102102080A CN102102080A (en) | 2011-06-22 |
| CN102102080B true CN102102080B (en) | 2013-07-03 |
Family
ID=44155150
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010564824 Expired - Fee Related CN102102080B (en) | 2010-11-24 | 2010-11-24 | Automatic biological component extracting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102102080B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045467B (en) * | 2011-10-13 | 2014-05-14 | 徐仰德 | Biomedicine homogenizing apparatus |
| CN109879203A (en) * | 2019-04-23 | 2019-06-14 | 北京旷视机器人技术有限公司 | Lifting mechanism and transfer robot with it |
| CN111394220B (en) * | 2020-03-24 | 2023-05-02 | 北京康彻思坦生物技术有限公司 | Nucleic acid extraction device |
| CN113337381B (en) * | 2021-06-30 | 2022-09-30 | 成都导胜生物技术有限公司 | Automatic grinding device and application thereof in obtaining single cells |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0469504B1 (en) * | 1990-08-03 | 1994-03-30 | GUIGAN, Anne | Device for centrifugally separating two phases of a heterogeneous liquid, in particular for use in the separation of blood plasma |
| EP0745562A2 (en) * | 1995-05-31 | 1996-12-04 | Masatoshi Matsumura | Purification system for groundwater or wastewater; biochemical reactor and liquid-transport stirring apparatus |
| CN2442270Y (en) * | 2000-09-12 | 2001-08-08 | 淄博中保康医疗器具有限公司 | Centrifugal separator of plasma single collection |
| CN2544807Y (en) * | 2002-04-25 | 2003-04-16 | 吴懿平 | Cryogenic vibration superfine disintegrator |
| CN201380105Y (en) * | 2009-04-01 | 2010-01-13 | 山东省农业科学院中心实验室 | Rapid separating machine for sweet potato starch |
-
2010
- 2010-11-24 CN CN 201010564824 patent/CN102102080B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0469504B1 (en) * | 1990-08-03 | 1994-03-30 | GUIGAN, Anne | Device for centrifugally separating two phases of a heterogeneous liquid, in particular for use in the separation of blood plasma |
| EP0745562A2 (en) * | 1995-05-31 | 1996-12-04 | Masatoshi Matsumura | Purification system for groundwater or wastewater; biochemical reactor and liquid-transport stirring apparatus |
| CN2442270Y (en) * | 2000-09-12 | 2001-08-08 | 淄博中保康医疗器具有限公司 | Centrifugal separator of plasma single collection |
| CN2544807Y (en) * | 2002-04-25 | 2003-04-16 | 吴懿平 | Cryogenic vibration superfine disintegrator |
| CN201380105Y (en) * | 2009-04-01 | 2010-01-13 | 山东省农业科学院中心实验室 | Rapid separating machine for sweet potato starch |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102102080A (en) | 2011-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102102080B (en) | Automatic biological component extracting device | |
| CN102093952B (en) | Device for automatically extracting biological component on the basis of electromagnetic propulsion grinding and centrifugal separation | |
| KR20110106873A (en) | Automated system for the lysis of microorganisms in a sample, and for the extraction and purification of the nucleic acids of said microorganisms for analysis | |
| WO2007006049A2 (en) | Apparatuses, systems, and methods for isolating and separating biological materials | |
| US20110086426A1 (en) | Methods and apparatus for collecting and separating regenerative cells from adipose tissue | |
| CN104893969A (en) | Automatic plasmid extracting device with compact structure | |
| WO2017141394A1 (en) | Reaction device capable of performing centrifugation | |
| CN202033241U (en) | Cam-driven biological sample freezing and grinding device | |
| KR20170120782A (en) | Extraction and purification apparatus | |
| JP2004337137A (en) | Automatic nucleic acid extraction and automatic nucleic acid extractor | |
| CN109465077A (en) | A kind of bioengineering mosses leaflet chlorophyll extraction pretreatment unit | |
| KR101044371B1 (en) | Shaker for Isolating stem cell | |
| CN101975863B (en) | In-situ grinding and centrifuging device | |
| CN109201346A (en) | A kind of supercentrifuge for food inspection | |
| CN110628573B (en) | Potato nucleic acid extraction device and method | |
| KR101544076B1 (en) | Centrifugal separator | |
| CN109540618A (en) | The method, apparatus and system particulate matter is separated from filter material matrix, extracted | |
| CN115322875B (en) | Stem cell pretreatment device and method | |
| CN203459170U (en) | High-speed centrifuge rotor mechanism capable of refrigerating | |
| CN112779133A (en) | Refining device for nucleic acid extraction and working method thereof | |
| CN107418871A (en) | A kind of preparation method of self-reacting device for enrichment cycles tumour cell | |
| CN207662688U (en) | A kind of pink reineckea herb amino acid extraction element | |
| CN205700939U (en) | A kind of dismembyator being applicable to insecticide | |
| CN213708373U (en) | A separation and purification device for RNA | |
| CN211838499U (en) | Centrifugal device suitable for nucleic acid grinding and separating |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130703 Termination date: 20181124 |