CN103045467B

CN103045467B - Biomedicine homogenizing apparatus

Info

Publication number: CN103045467B
Application number: CN201110309445.4A
Authority: CN
Inventors: 徐仰德
Original assignee: Individual
Current assignee: Individual
Priority date: 2011-10-13
Filing date: 2011-10-13
Publication date: 2014-05-14
Anticipated expiration: 2031-10-13
Also published as: CN103045467A

Abstract

The invention provides a biomedicine homogenizing apparatus, comprising a base body, a rotating assembly and at least one accommodating component, wherein the base body is provided with at least one power wheel, and the power wheel is arranged at an inner wall of the base body; the rotating component can be rotatably assembled with the power wheel, the rotating component comprises a driving shaft and two rotary discs, the two rotary discs are respectively arranged the two ends close to the driving shaft, the two ends of the driving shaft are respectively and mutually assembled with two groups of power wheels, the two rotary discs are respectively provided with a group of driven wheels, and the power wheels drive the driven wheels to rotate relative to the rotary discs; and the accommodating components can be rotationally disposed between the two rotary discs and are mutually assembled with the driven wheels. According to the biomedicine homogenizing device provided by the invention, the repeated impact frequency of a magnetic bead in a micropipe can be increased under a fixed rotation speed, and thus the effect for improving the substance homogenization can be realized.

Description

Biomedicine homogenizing device

Technical field

The present invention is about a kind of Biomedicine homogenizing device, and particularly a kind of have a Biomedicine homogenizing device that promotes homogenizing effect and have low temperature simultaneously.

Background technology

In the research field of molecular biosciences, often various species DNA, RNA or protein to be tested or that identify need be extracted, can be carried out detection and the observation of follow-up every specificity factor.Generally speaking, such as the material such as various tissues (for example: skin, liver, kidney etc.), cell, vaccine, other impurity all may adulterate in the time of first processing, even excessive cannot being directly used of volume because organizing, therefore the homogeneous manner that adopt are processed more conventionally, make it go for the program of follow-up screening or purifying.

The gimmick of tradition homogeneous can adopt the modes such as high speed centrifugation, ultrasonic oscillation or facility cutting.Wherein, to organize homogeneous to turn to example; conventionally the fragment of tissue of wanting homogeneous is installed in a microtubule; cut by the facility of head tool blade again; with in running up down, the fragment of tissue in this microtubule is sucked to the blade part of these facility, and in spatial joint clearance, shear, the action such as dispersion; and the head side of these facility produces high pressure ejection certainly, to reach fast the micronized effect of fragment of tissue.Only, easily produce the residual phenomenon of a large amount of fragments of tissue in this facility head by the mode of this tradition facility cutting, and the effect that causes fragment of tissue to homogenize is imperfect; And, more may be careless because of the cleaning of this facility head, and under the reusing of these facility the problem such as the pollution of generation fragment of tissue, raw bacterium, have a strong impact on the experiment quality of this fragment of tissue of subsequent operations.

Therefore, at present many by ultracentrifugal method organize, the operation that homogenizes of the material such as cell, and also developed on the market the clarifixator that has high-speed centrifugal, the Bullet BlenderTM clarifixator of being sold as Zhi De international corporation.To organize homogeneous to turn to example, it is prior to being filled with in the microtubule of the tissue of wanting to homogenize, put in addition at least one magnetic bead, again this microtubule is positioned in the set slot of this clarifixator, and by the high speed centrifugation of this clarifixator, make this magnetic bead in this microtubule, repeatedly to clash into the tissue of wanting to homogenize, reach the object of organizing fragmentation, homogeneous.

But the clarifixator of above-mentioned high-speed centrifugal only can drive the slot synchronous rotary of putting this microtubule, and produces ultracentrifugal effect.So, this magnetic bead produces the frequency limited of clashing into repeatedly in the centrifugal rotational speed of this clarifixator in this microtubule, and cannot under fixed rotating speed, increase the repeatedly collision frequency of this magnetic bead in microtubule again, causes the effect that tissue homogenizes to be subject to significant limitation; Even, in the situation of synchronous high-speed centrifugal rotation, easily make this magnetic bead fall within the bottom of this microtubule, swing and only can produce microseism frequently in this microtubule bottom, all the time the tissue of wanting to homogenize in this microtubule of shock that cannot be comprehensive, have a strong impact on the effect that this tissue homogenizes, and this homogeneous structure of subsequent operations screens, the experiment quality of purifying or evaluation.

In view of this, be really necessary to develop a kind of Biomedicine homogenizing device that promotes homogenizing effect, with synchronously and independently high speed centrifugation of the box body by taking up microtubule, solve variety of issue as above.

Summary of the invention

Main purpose of the present invention is to improve above-mentioned shortcoming, and so that a kind of Biomedicine homogenizing device to be provided, it can increase the repeatedly collision frequency of magnetic bead in microtubule, the effect homogenizing to promote material under fixed rotating speed.

The present invention's time object is to provide a kind of Biomedicine homogenizing device, and the material of wanting to homogenize in shock microtubule that can be comprehensive, with the effect of the complete homogeneous of tool material and maintain the quality of subsequent experimental.

Still a further object of the present invention is to provide a kind of Biomedicine homogenizing device, can complete homogeneous operation in low temperature environment, to promote homogenizing effect and device usability.

For reaching aforementioned goal of the invention, Biomedicine homogenizing device of the present invention, comprises: a pedestal, be provided with at least one power wheel, and this power wheel is arranged at the inwall of this pedestal; One rotary components, be assembled in rotationally this pedestal, this rotary components comprises a main drive shaft and two rotating disks, this main drive shaft and this power wheel mutually group connect, two ends of this main drive shaft are respectively arranged with this two rotating disk, and have at least a rotating disk to be provided with at least one follow-up pulley, this power wheel drives follow-up pulley with respect to turntable rotation; And at least one accommodating member, be arranged at rotationally between this two rotating disk, and with this follow-up pulley mutually group connect.

Biomedicine homogenizing device of the present invention can be selected to be provided with two groups of power wheels in this pedestal, and these two groups of power wheels are arranged at respectively the corresponding inwall of this pedestal, and respectively organizes power wheel and be jointly formed by stacking by the power wheel of the two identical numbers of teeth.And, be all provided with one group of follow-up pulley in this two rotating disk, respectively organize follow-up pulley and formed by the follow-up pulley of the two identical numbers of teeth, and each group of follow-up pulley of this two rotating disk is mutual dislocation configuration.

Wherein, two ends of this main drive shaft can run through respectively this two groups of power wheels, and are provided with a propulsion source in wherein one end of this main drive shaft.

Wherein, this two rotating disk can be sticked in respectively each Access Division of these main drive shaft two ends, and is fixed by a clasp.

Moreover, this follow-up pulley can have a rotation section and a group connecting part, and this rotation section can be with respect to this turntable rotation, and this group connecting part can be located at the inner face of this rotating disk, can be assembled for this accommodating member by this group connecting part, to limit this accommodating member between this two rotating disk.

Wherein, this two rotating disk be not provided with this follow-up pulley locate be separately provided with array fitting, this array fitting can be with respect to this turntable rotation.

Wherein, one end of this accommodating member can be mounted on the group connecting part of this follow-up pulley, and the other end of this accommodating member is mounted on the connecting piece of this rotating disk.

Wherein, in this accommodating member, can distinguish several tanks, this is counted tank and is sealed with a cover plate, and this cover board hub is in the periphery of this accommodating member.

In addition, Biomedicine homogenizing device of the present invention can also be provided with a magnetic part in this main drive shaft periphery wall, and this magnetic part is coated on this main drive shaft periphery wall, in order to magnetic force to be provided.

In addition, Biomedicine homogenizing device of the present invention, more can separately arrange a gas circulation assembly in this pedestal, so as to controlling the air themperature of this pedestal inside, and the better homogeneous environment that is maintained at 4 ℃.

The present invention is by the common connection configuration of this power wheel and follow-up pulley, make this rotary components can drive easily this accommodating member to be synchronously and independently rotation, under the rotating speed in stable, increase the toggle frequency of this accommodating member, promote whereby the repeatedly collision frequency of this magnetic bead in this microtubule, and the material of wanting to homogenize in this microtubule of shock that can be comprehensive reaches and promotes effect that material homogenizes.

Accompanying drawing explanation

Fig. 1 is the three-dimensional exploded view of Biomedicine homogenizing device of the present invention.

Fig. 2 is the combining and configuring schematic perspective view of Biomedicine homogenizing device of the present invention.

Fig. 3 is the partial component schematic diagram of Biomedicine homogenizing device of the present invention.

Fig. 4 is the combining and configuring diagrammatic cross-section of Biomedicine homogenizing device of the present invention.

Fig. 5 is the illustrative view of Biomedicine homogenizing device of the present invention.

[main element nomenclature]

(the present invention)

1 pedestal 11 power wheel 2 rotary components 21 main drive shafts

211 Access Division 212 clasp 213 magnetic part 22 rotating disks

221 connecting piece 23 follow-up pulley 231 rotating part 232 group connecting parts

Accommodating member 31 joining hole 32 tanks of 24 moving part 3

33 cover plate 4 microtubule 41 magnetic bead 5 gas circulation assemblies

6 regulation piece S accommodation space W get thing window B and open and close cover plate

M propulsion source

Embodiment

For above-mentioned and other object of the present invention, feature and advantage can be become apparent, preferred embodiment of the present invention cited below particularly, and coordinate accompanying drawing, be described in detail below:

Please refer to shown in Fig. 1, Biomedicine homogenizing device of the present invention comprises a pedestal 1, a rotary components 2 and at least one accommodating member 3, and this rotary components 2 is assembled in this pedestal 1 rotationally, and this rotary components 2 is in order to drive the rotation of this accommodating member 3.Whereby, several microtubules 4 (as shown in Figure 3) can be positioned in this accommodating member 3, to produce in synchronous rotary and independent rotation with this rotary components 2 in this accommodating member 3, increase magnetic bead 41 collision frequencies in each this microtubule 4, reach effect of the comprehensive fragmentation of material, homogeneous.

Wherein, the configuration quantity of this accommodating member 3, determines the design kenel of Biomedicine homogenizing device of the present invention, so that respectively this accommodating member 3 all can produce synchronous rotary and independent rotation, and magnetic bead 41 collision frequencies that increase in each this microtubule 4 are main principle.This accommodating member 3 can be at least one group, only the magnitude setting of this accommodating member 3 is not restricted, in order to reach the better effect of fragmentation as comprehensive in above-mentioned substance, homogeneous, and under the same time, can provide a large amount of microtubules 4 to place, the present invention selects to be provided with four accommodating members 3 in the mode of balanced configuration, and to should four accommodating members 3 and adopt design as described below as preferred embodiment of the present invention, please coordinate referring to figs. 1 through shown in 5, with under be elaborated.

Please refer to shown in Fig. 1, it is a preferred embodiment of the present invention, this Biomedicine homogenizing device comprises a pedestal 1, a rotary components 2 and four accommodating members 3, and this rotary components 2 is assembled in this pedestal 1 rotationally, and this rotary components 2 is in order to drive the respectively rotation of this accommodating member 3.

This pedestal 1 is only provided as this rotary components 2 and supports or accommodating use, therefore do not limit in this form that this pedestal 1 should present.Especially as shown in Figure 2, the pedestal 1 of the present embodiment can have an accommodation space S, uses for this rotary components 2 and is assembled in this accommodation space S.And, can offer one in this pedestal 1 and get thing window W, this is got, and thing window W is better to be given closely sealed with a keying cover plate B.Even, more can optionally organize separately other equipment (for example: gas circulation assembly etc.) are housed in this pedestal 1, so that Biomedicine homogenizing device of the present invention can be used comparatively widely, or use under a low temperature environment.

Please coordinate with reference to shown in Fig. 1 and 2, this pedestal 1 is provided with at least one power wheel 11, and this power wheel 11 can be driven by a propulsion source, in the middle of the present embodiment, this propulsion source M can be a motor, and this at least one power wheel 11 forms two groups, and is arranged at respectively the corresponding inwall of this pedestal 1.This at least one group of power wheel 11 can be made up of jointly two power wheels, and better selection is superimposed with each other by two gears of the identical number of teeth of tool, so that this rotary components 2 drives when respectively this accommodating member 3 rotates, can make respectively this accommodating member 3 be the operation that homogenizes of identical centrifugal rotational speed.

This rotary components 2 is assembled in this pedestal 1 rotationally, better being connected between these two groups of power wheels 11.The rotary components 2 of the present embodiment comprises a main drive shaft 21 and two rotating disks 22, this main drive shaft 21 connects with the mutual group of these two groups of power wheels 11, run through respectively this two groups of power wheels 11 by two ends of this main drive shaft 21 especially, and be provided with a propulsion source M in wherein one end of this main drive shaft 21, to be driven the rotation of this rotary components 2 by this propulsion source M outputting power.Again and, two ends of this main drive shaft 21 are respectively arranged with this two rotating disk 22, this two rotating disk 22 can form non-positive toroidal with the joint portion of main drive shaft 21, the two can synchronously rotate to make this, in the middle of the present embodiment, respectively establish an Access Division 211 in these main drive shaft 21 2 ends and be socketed for this two rotating disk 22, and fixed by a clasp 212, to guarantee that this two rotating disk 22 can be synchronous rotation with this main drive shaft 21.

In addition, the present invention can also select to be provided with a magnetic part 213 in these main drive shaft 21 periphery walls, and as shown in the drawing of Fig. 1, the better periphery wall that is coated on this main drive shaft 21 of this magnetic part 213, provides magnetic force to attract the magnetic bead 41 in this microtubule 4 whereby.Wherein, the form that arranges of this magnetic part 213 is selected but is not restricted to complete coated this main drive shaft 21, and the personage that genus is had the knack of this skill can understand easily, no longer in this detailed description.

Please shown in Figure 1 again, the present invention can select but the wherein rotating disk 22 in this rotary components 2 of not limiting is provided with at least one follow-up pulley 23, to engage these accommodating members 3 by this follow-up pulley 23.This follow-up pulley 23 can be driven by power wheel 11 and rotate with respect to rotating disk 22, and this induced way can be existing various induced way, for example: utilize gear be engaged in this two between, make open-bull gear drive; Or establish a ring internal tooth in these rotating disk 22 outer peripheral edges, this follow-up pulley 23 engages with this internal tooth; Or as shown in this embodiment, between this follow-up pulley 23 and power wheel 11 with the moving part such as chain, belt 24 driven rotary.In the middle of the present embodiment, this two rotating disk 22 is better to be all provided with one group of follow-up pulley 23 and (to form one group by two follow-up pulleys of the two identical numbers of teeth (Module), below summary is " follow-up pulley 23 "), and the better mutual dislocation that is of the follow-up pulley 23 of this two rotating disk 22 configures, special as shown in the drawing of Fig. 1, make the follow-up pulley 23 of this two rotating disk 22 mutually be the form that arranges of cross intersection, so that when respectively this accommodating member 3 is assembled, can make respectively this 3 of accommodating member leave more suitable spacing, avoid affecting the rotating effect when centrifugal.And, in this two rotating disk 22 be not provided with this follow-up pulley 23 locate be separately provided with array fitting 221, this connecting piece can be made position rotating with respect to rotating disk 22, and the connecting piece 221 on this rotating disk 22 is mutually corresponding with this follow-up pulley 23 on another rotating disk 22, in order to accept one end of this accommodating member 3.

Wherein, respectively this follow-up pulley 23 in this follow-up pulley 23 has all respectively a rotation section 231 and a group connecting part 232, this rotation section 231 can rotate with respect to this rotating disk 22, the better internal surface that is convexly set in this rotating disk 22 of this group connecting part 232, to be assembled for the other end of accommodating member 3 by this group connecting part 232, and limit this accommodating member 3 between this two rotating disk 22, and make that respectively the rotation section 231 of this follow-up pulley 23 and the power wheel 11 of this pedestal 1 are corresponding, to be bonded with each other by a moving part 24.

The configuration of organizing power wheel 11 more and organizing follow-up pulley 23 more in order to adapt to the present embodiment, the rotary components 2 of Biomedicine homogenizing device of the present invention selects to be provided with several moving parts 24, this counts moving part 24 respectively around being arranged in this power wheel 11 and follow-up pulley 23, to be driven this power wheel 11 and follow-up pulley 23 to rotate by the around the movement of this moving part 24, special in the number of teeth of this power wheel 11 is greater than the number of teeth of this follow-up pulley 23, and can force this follow-up pulley 23 to do the rotation of more revolutions.

Please coordinate with reference to shown in Fig. 1 and 3, respectively this accommodating member 3 is arranged between this two rotating disk 22 rotationally, goodly establish a joining hole 31 in each two corresponding sidewalls of this accommodating member 3, make the each joining hole 31 of these accommodating member 3 one end be mounted on the group connecting part 232 of this follow-up pulley 23, and respectively 31 of the joining holes of these accommodating member 3 the other ends are mounted on the connecting piece 221 of this rotating disk 22, make the rotation of this accommodating member 3 more balanced and firm.Wherein, the shape of this accommodating member 3 and size design, only take 22 of two rotating disks that can take up several microtubules 4 and be hubbed at this rotary components 2 as main principle, therefore do not limited in this form that this accommodating member 3 should present.

Specifically, as shown in Figure 3, it is one box-like that this accommodating member 3 forms, this box-like interior differentiation of accommodating member 3 has several tanks 32, and the respectively wide design in footpath of this microtubule 4 of the wide cooperation in the footpath of this tank 32 so that this microtubule 4 can be placed in this tank 32, and is not better principle in the excessive double swerve of the interior generation of this tank 32, the personage that genus is had the knack of this skill can understand easily, no longer adds to repeat.And, several tanks 32 of this accommodating member 3 are sealed with a cover plate 33, the better periphery that is hubbed at this accommodating member 3 of this cover plate 33, so that open and closure, especially can in the time of closure, reach preferably fastening effect, while being unlikely to high speed centrifugation, occur that this cover plate 33 gets loose and the situation that causes microtubule 4 to drop.

It should be noted that, Biomedicine homogenizing device of the present invention can be from the above, can also be in the interior gas circulation assembly 5 (as shown in Figure 2) that separately arranges of this pedestal 1, to control the air themperature in this pedestal 1 by this gas circulation assembly 5, make Biomedicine homogenizing device of the present invention that the different environment that homogenizes can be provided, under the better low temperature situation that is maintained at 4 ℃, to be widely used in various animal tissuess, cell or bacterial classification.And, more can be in the outer wall configurations of this pedestal 1 regulation piece 6, with the panel control menu by this regulation piece 6, grasp Biomedicine homogenizing device of the present invention in the high speed centrifugation rotating speed of homogenizing process, and the applicable envrionment temperature of various animal tissuess, cell or bacterial classification, reach effect that preferably material homogenizes.

Please refer to shown in Fig. 4 and 5, it only configures the one form of this pedestal 1, rotary components 2 and the accommodating member 3 of number for Biomedicine homogenizing device of the present invention, to illustrate that whereby the present invention is in the start situation of homogenizing process.

Before operation Biomedicine homogenizing device of the present invention, first select the material of wanting to homogenize (for example: animal tissues, cell or bacterial classification etc.) to make an addition in this microtubule 4, and in this microtubule 4, insert at least one magnetic bead 41 (as shown in Figure 3) simultaneously, several the microtubules 4 that again configuration completed are positioned over respectively in the tank 31 of each this accommodating member 3, and set suitable rotating speed and temperature by this regulation piece 6, with the operation homogenizing simultaneously.So, rotate with the main drive shaft 21 that drives this rotary components 2 by this propulsion source M outputting power, the accommodating member 3 of number that makes to be located at 22 of two rotating disks of this rotary components 2 can be synchronous rotation; Simultaneously, the follow-up pulley 23 that is arranged at this two rotating disk 22 can be by power wheel 11, moving part 24, make the accommodating member 3 of this number produce rotation relatively to this two rotating disk 22, the accommodating member 3 that is connected to this follow-up pulley 23 not only can be outside synchronous rotation with this rotary components 2, more can drive this accommodating member 3 to be independent rotation by this follow-up pulley 23, under the rotating speed in stable, increase the toggle frequency of this accommodating member 3, this magnetic bead 41 can repeatedly be clashed in this microtubule 4, and reach effect of complete homogeneous.

In sum, the principal character of Biomedicine homogenizing device of the present invention is: by the common connection configuration of this power wheel 11 and follow-up pulley 23, make this rotary components 2 can drive easily this accommodating member 3 to be synchronously and independently rotation, under the rotating speed in stable, increase the toggle frequency of this accommodating member 3, promote whereby the repeatedly collision frequency of this magnetic bead 41 in this microtubule 4, and the interior material of wanting to homogenize of this microtubule 4 of shock that can be comprehensive reaches and promotes effect that material homogenizes.Even, when Biomedicine homogenizing device of the present invention is during in middle high rotating speed operation, still can be by the toggle frequency that increases this accommodating member 3, and maintained preferably homogenizing effect; More can, because of long high speed rotating, not produce the situation that falls within these microtubule 4 bottoms as traditional magnetic bead 41, therefore Biomedicine homogenizing device of the present invention can not be limited to the speed of centrifugal rotational speed, and maintain equally effect of stable homogeneous.

Biomedicine homogenizing device of the present invention can increase the repeatedly collision frequency of magnetic bead in microtubule under fixed rotating speed, promotes to reach effect that material homogenizes.

The material of wanting to homogenize in the shock microtubule that Biomedicine homogenizing device of the present invention can be comprehensive, with the effect of the complete homogeneous of tool material, reaches the effect that maintains subsequent experimental better quality.

Biomedicine homogenizing device of the present invention can complete homogeneous operation in low temperature environment, to promote homogenizing effect, and is widely used in various animal tissuess, cell or bacterial classification, relatively reaches effect of lifting gear usability.

Claims

1. a Biomedicine homogenizing device, is characterized in that comprising:

A pedestal, is provided with at least one power wheel, and this power wheel is arranged at the inwall of this pedestal;

A rotary components, be assembled in rotationally this pedestal, this rotary components comprises a main drive shaft and two rotating disks, this main drive shaft and this power wheel mutually group connect, the two ends of this main drive shaft are respectively arranged with this two rotating disks, and have at least a rotating disk to be provided with at least one follow-up pulley, this power wheel drives this follow-up pulley with respect to turntable rotation; And

At least one accommodating member, is arranged between these two rotating disks rotationally, and with this follow-up pulley mutually group connect.

2. Biomedicine homogenizing device as claimed in claim 1, is characterized in that, this pedestal is provided with two groups of power wheels, and these two groups of power wheels are arranged at respectively the corresponding inwall of this pedestal, and respectively organizes power wheel and be jointly formed by stacking by the power wheel of two identical numbers of teeth.

3. Biomedicine homogenizing device as claimed in claim 2, is characterized in that, these two rotating disks are all provided with one group of follow-up pulley, respectively organize follow-up pulley and be made up of the follow-up pulley of two identical numbers of teeth, and each group of follow-up pulley of these two rotating disks is mutual dislocation configuration.

4. Biomedicine homogenizing device as claimed in claim 2 or claim 3, is characterized in that, this two groups of power wheels are run through respectively at the two ends of this main drive shaft, and are provided with a propulsion source in wherein one end of this main drive shaft.

5. the Biomedicine homogenizing device as described in claim 1,2 or 3, it is characterized in that, this follow-up pulley has a rotation section and a group connecting part, this rotation section this turntable rotation relatively, this group connecting part is located at the internal surface of this rotating disk, to be assembled for this accommodating member by this group connecting part, and limit this accommodating member between this two rotating disk.

6. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, this two rotating disk is not provided with this follow-up pulley part and is separately provided with several connecting pieces, and this connecting piece can be with respect to this turntable rotation.

7. Biomedicine homogenizing device as claimed in claim 6, is characterized in that, one end of this accommodating member is mounted on the group connecting part of this follow-up pulley, and the other end of this accommodating member is mounted on the connecting piece of this rotating disk.

8. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, this main drive shaft periphery wall is provided with one in order to the magnetic part of magnetic force to be provided, and this magnetic part is coated on this main drive shaft periphery wall.

9. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, a gas circulation assembly is separately set in this pedestal, in order to control the air themperature of this pedestal inside.

10. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, these two rotating disks are sticked in respectively the Access Division of this both ends of main drive shaft, and is fixed by a clasp.

11. the Biomedicine homogenizing device as described in claim 1,2 or 3, is characterized in that, distinguishes and has several tanks in this accommodating member, these several tanks are sealed with a cover plate, and this cover board hub is in the periphery of this accommodating member.