CN111394220B - Nucleic acid extraction device - Google Patents

Abstract

The invention discloses a nucleic acid extraction device. The nucleic acid extraction device comprises a base, a centrifugal module and a mixing module, wherein the centrifugal module comprises a centrifugal tray, the centrifugal tray is rotatably arranged on the base around a centrifugal axis, the mixing module comprises a test tube fixing piece, the test tube fixing piece is provided with a test tube accommodating hole for accommodating a test tube, the test tube fixing piece is rotatably arranged on the centrifugal tray around a mixing axis, and the mixing axis is obliquely arranged relative to the centrifugal axis. According to the nucleic acid extraction device, the test tube fixing part rotates relative to the centrifugal tray, and the mixing axis is inclined relative to the centrifugal axis, so that the rotating speed of the test tube fixing part has a component in the vertical direction, and reagents in the test tube can be enabled to perform upside down movement so as to better mix the reagents.

Description

Nucleic acid extraction device

technical field

The invention relates to the field of bioengineering, in particular to a nucleic acid extraction device.

Background technique

Nucleic acid is the most basic substance of life. It is composed of biological macromolecular compounds and is crucial to the entire living system. No matter in practical application or in scientific research, nucleic acid has extremely important applications. Many genetic diseases are closely related to nucleic acid. In general, the study of nucleic acids is of great significance.

As a basic method of biomolecules, nucleic acid extraction is the starting point of downstream nucleic acid detection, research analysis and product development, and is also a key step in nucleic acid diagnosis. Nucleic acid extraction has high requirements on the quality and integrity of the isolated nucleic acid, which has a crucial impact on both research and diagnosis. Therefore, fast, effective and accurate nucleic acid extraction technology is a prerequisite for subsequent research. The aqueous two-phase system is a two-phase or multi-phase system that is immiscible after certain polymers or polymers and salts or other combinations are mixed in water at a certain concentration. The two-phase aqueous nucleic acid extraction method is a new type of nucleic acid extraction technology, which uses the difference in the distribution coefficient of the solute in the two phases to extract the target nucleic acid to achieve nucleic acid extraction.

According to the operating requirements of the two-phase nucleic acid extraction system for nucleic acid extraction, various instruments such as centrifuges, heating plates, and oscillators are often required, accompanied by cumbersome manual operations. At present, most nucleic acid extraction instruments still use traditional nucleic acid extraction methods. The overall extraction reaction time is long, the efficiency is low, the operation is cumbersome, the cost is high, and the degree of automation is low.

Contents of the invention

The purpose of the present invention is to provide a nucleic acid extraction device to better mix the reagents.

The invention provides a nucleic acid extraction device, comprising:

base;

The centrifugal module includes a centrifugal tray, which is rotatably arranged on the base around the centrifugal axis; and

The mixing module includes a test tube fixing part, the test tube fixing part has a test tube receiving hole for accommodating the test tube, and the test tube fixing part is rotatably arranged on the centrifugal tray around the mixing axis, and the mixing axis is inclined relative to the centrifugal axis.

In some embodiments, the inclination angle of the mixing axis relative to the centrifugal axis ranges from 15° to 45°.

In some embodiments, the axis of the tube receiving aperture intersects the mixing axis.

In some embodiments, the axis of the tube receiving aperture is perpendicular to the mixing axis.

In some embodiments, the test tube holder is configured to perform periodic forward and reverse rotations relative to the centrifuge tray.

In some embodiments, the nucleic acid extraction device comprises two mixing modules arranged symmetrically with respect to the centrifuge axis.

In some embodiments, the centrifugal tray includes a central disk body and inclined disk bodies arranged on both sides of the central disk body, and two mixing modules are correspondingly arranged on the two inclined disk bodies.

In some embodiments, the mixing module further includes a housing arranged on the outside of the test tube holder. The housing includes a tank and a cover that is rotatably connected to the tank. The inner wall of the cover is provided with a test tube fastening strip. When the cover When the body rotates to the position of closing the groove body, the test tube fastening bar abuts against the test tube cover of the test tube to compress the test tube cover.

In some embodiments, the test tube fixing member has a plurality of test tube receiving holes arranged at intervals, and the test tube fastening strip has a limiting groove, and the limiting groove extends along the distribution direction of the plurality of test tube receiving holes so that the test tube cover is clamped on the in the limit groove.

In some embodiments, an elastic material is installed in the limiting groove.

In some embodiments, the test tube fixing member includes connecting edges located on both sides of the bottom, and the mixing module further includes spacers disposed between the connecting edges and the bottom surface of the housing, and the connecting edges are connected to the housing through the spacers.

In some embodiments, the nucleic acid extraction device further includes a heating module, the heating module includes a heating element, and the heating element heats the test tube fixing element.

In some embodiments, the heating element is an electrothermal film, and the electrothermal film is attached to the end surface of the test tube fixing element.

In some embodiments, the heating module further includes a temperature sensor, and the test tube fixing member further includes a detection hole disposed between two adjacent test tube accommodation holes, and a temperature sensor is placed in the detection hole.

In some embodiments, the nucleic acid extraction device further includes a heat dissipation module, wherein the heat dissipation module includes a side cooling fan arranged on the side of the test tube holder; and/or, the heat dissipation module further includes a bottom cooling fan arranged on the bottom surface of the test tube holder.

In some embodiments, the hybrid module includes a casing, and fan cooling holes are arranged on the casing.

In some embodiments, the nucleic acid extraction device further includes a control module. The control module includes a main controller coupled to the centrifugation module, a secondary controller coupled to the mixing module, and a conductive slip ring. The conductive slip ring is connected to the centrifuge tray and controls the main control. controller and sub-controller electrical connection.

Based on the technical solution provided by the present invention, the nucleic acid extraction device includes a base, a centrifugal module and a mixing module, the centrifugal module includes a centrifugal tray, the centrifugal tray is rotatably arranged on the base around the centrifugal axis, and the mixing module includes a test tube holder, a test tube holder There are test tube accommodating holes for accommodating test tubes, and the test tube fixing member is rotatably arranged on the centrifugal tray around the mixing axis, and the mixing axis is inclined relative to the centrifugal axis. The test tube fixing part of the nucleic acid extraction device of the present invention rotates relative to the centrifugal tray and the mixing axis is inclined relative to the centrifugal axis, so the rotating speed of the test tube fixing part has a vertical component so that the reagent in the test tube moves up and down to better control Reagents were mixed.

Other features of the present invention and advantages thereof will become apparent from the following detailed description of exemplary embodiments of the present invention with reference to the accompanying drawings.

Description of drawings

The accompanying drawings described here are used to provide a further understanding of the present invention and constitute a part of the application. The schematic embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations to the present invention. In the attached picture:

Fig. 1 is the structural representation of the nucleic acid extraction device of the embodiment of the present invention;

Fig. 2 is a schematic structural view of the centrifugal module in Fig. 1;

Fig. 3 is the structural representation of mixing module and centrifugal tray in Fig. 1;

Fig. 4 is a schematic diagram of the connection structure between the mixing module and the centrifuge tray in Fig. 3;

Fig. 5 is a schematic structural diagram of a mixing module and a heating module inside the housing in Fig. 3;

Fig. 6 is a schematic diagram of the structure of the heating module in Fig. 5 except for the cooling fan;

Fig. 7 is a schematic structural diagram of the control module in Fig. 1;

FIG. 8 is a schematic diagram of the control principle of the control module shown in FIG. 7 .

Each reference sign stands for:

1. Base;

2. Centrifugal module;

21. Centrifugal tray; 211. Central disc body; 212. Inclined disc body; 213. Vertical disc body; 22. DC motor; 221. DC motor output shaft; 23. Coupling; 25. Extended shaft; 26. Shaft Set; 27, motor tray; 28, motor bracket;

3. Hybrid module;

31. Shell; 311. Tank body; 311A. Fan cooling hole; 311B. Ventilation cooling hole; 312. Cover body; 32. Test tube fixing piece; 321. Test tube receiving hole; 322. Connection edge; 33. Pad block; 34 , lock; 35, stepping motor; 36, bearing holder, 37, bearing; 38, stepping motor shaft sleeve; 39, test tube fastening strip;

4. Control module;

41. Main controller; 42. Secondary controller; 43. Conductive slip ring;

5. Heating module;

6. Cooling module;

61. Side cooling fan; 62. Bottom cooling fan.

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. The following description of at least one exemplary embodiment is merely illustrative in nature and in no way taken as limiting the invention, its application or uses. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

The relative arrangements of components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. At the same time, it should be understood that, for the convenience of description, the sizes of the various parts shown in the drawings are not drawn according to the actual proportional relationship. Techniques, methods, and devices known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, techniques, methods, and devices should be considered part of the authorized description. In all examples shown and discussed herein, any specific values should be construed as exemplary only, and not as limitations. Therefore, other examples of the exemplary embodiment may have different values. It should be noted that like numerals and letters denote like items in the following figures, therefore, once an item is defined in one figure, it does not require further discussion in subsequent figures.

For the convenience of description, spatially relative terms may be used here, such as "on ...", "over ...", "on the surface of ...", "above", etc., to describe the The spatial positional relationship between one device or feature shown and other devices or features. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, devices described as "above" or "above" other devices or configurations would then be oriented "beneath" or "above" the other devices or configurations. under other devices or configurations". Thus, the exemplary term "above" can encompass both an orientation of "above" and "beneath". The device may be positioned in other different ways and the spatially relative descriptions used herein interpreted accordingly.

As shown in Figures 1 to 6, the nucleic acid extraction device of the embodiment of the present invention includes:

base1;

The centrifugal module 2 includes a centrifugal tray 21, and the centrifugal tray 21 is rotatably arranged on the base 1 around the centrifugal axis; and

The mixing module 3 includes a test tube fixing part 32, the test tube fixing part 32 has a test tube receiving hole 321 for accommodating the test tube A, and the test tube fixing part 32 is rotatably arranged on the centrifugal tray 21 around the mixing axis, and the mixing axis is inclined relative to the centrifugal axis set up.

The test tube holder 32 of the nucleic acid extraction device of the embodiment of the present invention rotates relative to the centrifugal tray 21 and the mixing axis is inclined relative to the centrifugal axis, so the rotation speed of the test tube holder 32 has a vertical component so that the reagent in the test tube A is upside down Movement to better mix the reagents.

In order to make the reagent have a better centrifugation effect, the inclination angle of the mixing axis relative to the centrifugation axis in this embodiment ranges from 15° to 45°.

As shown in FIG. 3 , the mixing module 3 of this embodiment further includes a housing 31 disposed outside the test tube fixing member 32 and fixedly connected to the test tube fixing member 32 . The casing 31 has a square structure and includes a tank body 311 and a cover body 312 rotatably connected to the tank body 311 . The cover body 312 is connected to the groove body 311 through a lock 34 to securely lock the housing body 31 . Specifically, the locking buckle 34 includes a box buckle disposed on the tank body 311 and a hook disposed on the cover body 312, and the hook cooperates with the box buckle.

In the following description, the side of the cover 312 is referred to as an upper side, and the side opposite to the cover 312 is referred to as a lower side. As shown in Figure 6, the test tube fixing member 32 of the present embodiment includes connecting edges 322 positioned on both sides of the bottom, and the mixing module 3 also includes a spacer 33 arranged between the connecting edges 322 and the bottom surface of the housing 31, the connecting edges 322 It is connected with the housing 31 through the spacer 33 .

The mixing module 3 of this embodiment further includes a stepping motor 35 for driving the housing 31 to rotate. The output shaft of the stepping motor 35 is connected with the stepping motor shaft sleeve 38 , and the stepping motor shaft sleeve 38 is connected with the side wall of the housing 31 . The stepper motor shaft sleeve 38 is installed in the inner diameter of the bearing 37, and forms a tight fit with the inner ring structure of the bearing 37. The outer ring of the bearing 37 is installed in the bearing fixing seat 36, and the bearing fixing seat 36 is fixed on the centrifugal tray 21 by screws . The output shaft of the stepping motor drives the mixing module 3 to rotate to play the role of mixing reagents. And after the mixing module 3 is fixed, its weight is concentrated on the bearing fixing seat 36 , and the stepping motor 35 only plays the role of transmission, and will not bear the weight of the whole mixing module 3 .

In order to better mix the reagents, the test tube fixing member 32 in this embodiment is configured to perform periodic positive and negative rotations relative to the centrifuge tray 21 . Specifically, the output shaft of the stepping motor 35 is controlled to perform periodic forward and reverse rotations so as to drive the housing 3 and the test tube fixing member 32 to perform periodic forward and reverse rotations. As shown in Figure 6, the test tube holder 32 of this embodiment has a plurality of test tube accommodation holes 321 arranged at intervals, and the plurality of test tube accommodation holes 321 are arranged in sequence along a straight line, thereby realizing the operation of nucleic acid extraction for multiple reagents to improve efficiency .

In order to further mix the reagents, the axis of the test tube receiving hole 321 in this embodiment intersects with the mixing axis. With such arrangement, the reagents in the test tube A move in the axial direction of the test tube A so that the reagents are fully mixed. The test tube fixing member 32 of this embodiment includes a plurality of test tube receiving holes 321, wherein the axis of the test tube receiving hole may be in the same plane and intersects with the mixing axis, and the axis of the test tube receiving hole and the mixing axis may not be coplanar and mutually cross.

Specifically, the axis of the test tube receiving hole 321 is perpendicular to the mixing axis. When the axis of the test tube holding hole 321 was perpendicular to the mixing axis, the test tube A followed the test tube holder 32 and rotated around the mixing axis, and the linear velocity direction of the test tube A was the axis of the test tube holding hole 321, so the reagent velocity in the test tube A was also the same direction, when the test tube fixing member 32 follows the housing 31 to perform periodic forward and reverse rotations, the reagent in the test tube A will be reversed along the axial direction of the test tube A. This setting ensures that the reagent has sufficient space to move during the upside-down mixing process, and also ensures that the reagent is centrifuged at this angle, so that the reagent can obtain a better centrifugation effect.

The nucleic acid extraction device of this embodiment also includes a heating module 5 . The heating module 5 includes a heating element, and the heating element heats the test tube fixing element 32 .

Specifically, the test tube fixing member 32 in this embodiment is a metal block. The heating element is an electrothermal film, and the electrothermal film is attached to the end surface of the test tube fixing part 32 . Preferably, electric heating films are attached to both end surfaces of the test tube fixing member 32 in this embodiment so that the test tube receiving hole 321 is located between the two electric heating films to ensure heating stability. Moreover, the electrothermal film covers a large area of the surface of the metal block to improve heating efficiency.

In other embodiments, the heating element may also be an electric heating tube, an electric heating ceramic sheet and other workpieces capable of heating, and it is not limited to the way of using electric energy for heating.

The end surface of the test tube fixing member 32 in this embodiment is provided with a mounting groove, and the electrothermal film is embedded in the mounting groove to achieve a good heating effect.

The diameter of the test tube receiving hole 321 of this embodiment is slightly larger than the diameter of the test tube so that when the test tube is inserted into the test tube receiving hole 321, there can be a better fit between the test tube wall and the test tube receiving hole wall to ensure direct heat conduction . At the same time, the wall thicknesses between adjacent test tube accommodation holes are the same to ensure the uniformity and stability of heat conduction during the heating process, and to reduce the temperature difference between the holes.

In order to monitor the heating temperature, the heating module 5 of this embodiment also includes a temperature sensor, and the test tube fixing member 32 also includes a detection hole disposed between two adjacent test tube accommodation holes, and a temperature sensor is placed in the detection hole. For example, the detection hole is opened between the first test tube accommodation hole and the second test tube accommodation hole, which can better reflect the temperature of the first test tube accommodation hole and the second test tube accommodation hole. Since the electrothermal film is arranged symmetrically and the test tube holding hole is also set in the middle of the test tube holder, the heating process is also symmetrical, and the temperature transfer is also symmetrical, so the temperature between the first test tube holding hole and the second test tube holding hole It can also reflect the temperature between the third test tube holding hole and the fourth test tube holding hole.

In order to avoid the problem of reagent leakage caused by the increase of the internal temperature and pressure of the test tube A during the heating process, the mixing module 3 of this embodiment also includes a test tube fastening strip arranged on the inner wall of the cover body 312 39. When the cover body 312 is rotated to the position of closing the groove body 311, the test tube fastening strip 39 abuts against the test tube cover of the test tube A to press the test tube cover so as to prevent the test tube cover from being disengaged.

Specifically, the test tube fastening strip 39 of this embodiment has a limiting groove, and the limiting groove extends along the distribution direction of the plurality of test tube receiving holes 321 so that a plurality of test tube caps are clamped in the limiting groove.

In this embodiment, an elastic material is installed in the limiting groove. When the cover body 312 is closed, the elastic material is in contact with the test tube cover. By controlling the thickness of the elastic material, the elastic material is in contact with the test tube cover and produces a certain deformation to continuously exert pressure on the test tube cover to tighten the test tube.

After mixing the reagents, it is also necessary to cool down the reagents inside the test tube. In this embodiment, the nucleic acid extraction device further includes a cooling module 6, and the cooling module 6 includes a side cooling fan 61 arranged on the side of the heating module 5; and/or, the cooling module 6 includes a bottom cooling fan 62 arranged on the bottom surface of the heating module 5 .

As shown in FIG. 3 , fan cooling holes 311A are provided on the surface of the housing 31 corresponding to the cooling fan in this embodiment. Specifically, the side and bottom surfaces of the housing 31 are provided with fan cooling holes 311A.

The tank body 311 of this embodiment is a square structure, which includes four circumferentially arranged sides and a bottom surface, wherein one side is the connection side connected to the centrifugal tray 21, and fans are arranged on the other two opposite sides and the bottom surface thermal vias 311A. As shown in FIG. 3 , a strip-shaped hole is provided on the lower side of the fan cooling hole 311A, and the strip-shaped hole is used to pass through the power supply line of the cooling fan to facilitate the arrangement and layout of the line. Moreover, the strip-shaped hole can further improve the heat dissipation effect.

In order to further improve the heat dissipation effect, there are a plurality of ventilation and heat dissipation holes 311B on the side opposite to the connecting side of the groove body 311 in this embodiment.

In order to maintain the balance of centrifugation, as shown in FIG. 1 , the nucleic acid extraction device of this embodiment includes two mixing modules 3 arranged symmetrically with respect to the axis of centrifugation.

As shown in FIG. 2 , the centrifugal tray 21 includes a central disk body 211 and inclined disk bodies 212 arranged on both sides of the central disk body 211 , and two mixing modules 3 are correspondingly arranged on the two inclined disk bodies 212 .

The centrifuge module 2 of this embodiment also includes a centrifugal drive device for driving the centrifuge tray 21 to rotate. Specifically, the centrifugal driving device in this embodiment is a DC motor 22 , and the output shaft 221 of the DC motor 22 is connected to the extension shaft 25 through a coupling 23 . The end of the extension shaft 25 is sleeved with a shaft sleeve 26 , and the shaft sleeve 26 is connected with the centrifugal tray 21 .

In this embodiment, as shown in Figure 7 and Figure 8, the nucleic acid extraction device also includes a control module 4, the control module 4 includes a main controller 41 coupled with the centrifugal module 2, a secondary controller 42 coupled with the mixing module 3 and The conductive slip ring 43 is connected to the centrifugal tray 21 and electrically connected to the main controller 41 and the secondary controller 42 .

Wherein, the main controller 41 is arranged on the base 1 .

Because the mixing module 3 and the heating module 5 all rotate with the centrifugal tray 21, and the secondary controller 42 is used to control the mixing module 3 and the heating module 5, in order to reduce the gap between the control module and the mixing module 3 and the heating module 5 Wires are connected to simplify the device, and the secondary controller 42 of this embodiment rotates synchronously with the centrifuge tray 21 .

Specifically, the centrifugal tray 21 of this embodiment further includes a vertical disk body 213 connected to the inclined disk body 212 , and the secondary controller 42 is connected to the vertical disk body 213 and rotates following the vertical disk body 213 .

The structure and working process of the nucleic acid extraction device of the embodiment of the present invention will be described in detail below with reference to FIGS. 1 to 8 .

As shown in FIGS. 1 to 7 , the nucleic acid extraction device of this embodiment includes a base 1 , a centrifugal module 2 , a mixing module 3 , a control module 4 , a heating module 5 and a cooling module 6 . The base 1 is fixedly arranged, the centrifugal module 2 is rotatably arranged on the base 1 , and the mixing module 3 is rotatably arranged on the centrifugal module 2 .

The centrifugal module 2 includes a centrifugal tray 21 , a DC motor 22 , a shaft coupling 23 , an extension shaft 25 , a shaft sleeve 26 , a motor tray 27 and a motor bracket 28 . Two motor brackets 28 are fixed side by side on the base 1, the motor tray 27 is connected to the top of the motor bracket 8, the top of the DC motor 22 is connected to the motor tray 27 and there is a certain distance between the bottom of the DC motor 22 and the base 1. The output shaft 221 of the DC motor 22 passes through the motor tray 27 and is connected with the extension shaft 25 through the coupling 23 . The extension shaft 25 is inserted into the hole of the shaft sleeve 26 and connected with the shaft sleeve 26 through a threaded connection. The centrifugal tray 21 is fixed on the upper surface of the shaft sleeve 25 . The DC motor 22 has a wider speed adjustment range, and provides a larger torque to drive the centrifugal tray 21 to rotate at a low speed, and ensures a good centrifugal effect at a high speed.

The nucleic acid extraction device of this embodiment includes two mixing modules 3 arranged symmetrically with respect to the centrifugation axis, so that the left and right sides of the centrifugal turntable 21 of this embodiment are strictly symmetrical to ensure the stability of centrifugation.

As shown in Figures 3 to 6, the mixing module 3 of this embodiment includes a housing 31, a test tube holder 32, a pad 33, a lock 34, a stepper motor 35, a bearing holder 36, a bearing 37, a stepper motor shaft Cover 38 and test tube fastening strip 39.

As shown in FIG. 3 , the housing 31 includes a tank body 311 and a cover body 312 . The test tube fixing member 32 is fixedly disposed in the casing 31 . As shown in FIG. 5 , the test tube fixing member 32 is provided with a plurality of test tube receiving holes 321 sequentially arranged along a straight line, and the test tube A containing the reagent is placed in the test tube receiving holes 321 . And the test tube holder 32 includes connecting edges 322 on both sides of the bottom, the connecting edges 322 are connected to the bottom surface of the housing 31 and a spacer 33 is arranged between the connecting edge 322 and the bottom surface of the housing 31 so that the test tube fixing member 32 and the shell There is a certain distance between the bottom surfaces of the bodies 31 .

As shown in FIG. 3 , the end surface of the stepping motor 35 is fixed on the lower surface of the centrifugal tray 21 by screws. The output shaft of stepper motor 35 is put into the circular hole of stepper motor axle sleeve 38. The stepper motor shaft sleeve 38 is installed into the inner diameter of the bearing 37 and forms a tight fit with the inner ring structure of the bearing 37. The outer ring of the bearing 37 is installed in the bearing fixing seat 36, and the bearing fixing seat 36 is fixed on the centrifugal tray 21 by screws . Driven by the stepping motor 35 , the mixing module 3 realizes a circular motion relative to the centrifugal tray 21 through the bearing 37 to realize mixing of the reagents. And after the mixing module 3 is fixed, its weight is concentrated on the bearing fixing seat 36 , and the stepping motor 35 only plays the role of transmission, and will not bear the weight of the whole mixing module 3 .

The casing 31 rotates relative to the centrifugal tray 21 under the drive of the stepping motor 35 to realize the upside-down mixing of the reagents in the test tube A. In order to prevent the reagents from spilling during the mixing process, the mixing module 3 of this embodiment also includes a The test tube fastening strip 39 inside the cover body 312, when the cover body 312 rotates relative to the tank body 311 to close the tank body 311, the test tube fastening strip 3939 presses the tops of a plurality of test tubes A to prevent the test tube cover from coming off.

Specifically, the test tube fastening strip 39 in this embodiment is a long metal strip with a rectangular groove on it. A rubber strip with certain elasticity is installed in the groove. When the cover body 312 is closed, the test tube fastening strip 39 is in contact with the test tube fixed in the test tube holder 32 . In the actual test process, by controlling the thickness of the rubber strip, the rubber strip is in contact with the test tube cover to produce a certain deformation, and continuously exerts pressure on the test tube cover to tighten the test tube. At the same time, it reduces the risk of reagent leakage due to steam generated by the gas heated by high temperature in the test tube when the test tube cover is opened.

The nucleic acid extraction device of this embodiment further includes a heating module 5 , and the heating module 5 includes a heating element connected to the test tube fixing member 32 , and the heating element heats the test tube A through the test tube fixing member 32 .

Specifically, the heating element in this embodiment is an electric heating film, and both sides of the test tube fixing member 32 are attached with electric heating films. The test tube fixing part 32 of this embodiment is a block structure, and electric heating films are respectively embedded in the two ends thereof, which are arranged front and back, covering a large area of the surface of the test tube fixing part 32, and the test tube receiving hole 321 is sandwiched between two electric heating films. In the middle, to ensure the stability of heating.

The test tube fixing part 32 of this embodiment is also provided with a detection hole, a temperature sensor is placed in the detection hole, and the inside of the detection hole is filled with thermal conductive silicone grease to ensure that the temperature in the detection hole can better follow the temperature of the test tube fixing part 32. The detection hole is opened in the middle of two adjacent test tube accommodation holes, which can better reflect the temperature of the two test tube accommodation holes.

The heat dissipation module 6 of this embodiment includes a side cooling fan 61 arranged on the side of the test tube fixing member 32 and a bottom cooling fan 62 arranged on the bottom surface of the test tube fixing member 32 . In this embodiment, a fan cooling hole 311A is provided at a position corresponding to the cooling fan of the casing 31 . The side cooling fan 61 discharges air from the casing 31 to the outside. The cooling fan 62 on the bottom surface blows air into the casing 31 . During the cooling process, all cooling fans are turned on, the bottom cooling fan 62 blows air into the housing 31 , and the side cooling fans 61 on both sides exhaust air to the outside of the housing 31 . The air under the external room temperature is blown on the bottom surface of the test tube holder 32, and the heat of the test tube holder 32 is taken away by the wind, and is dissipated by the side cooling fans 61 on both sides, so as to achieve a good air cooling effect. And because there are corresponding fan cooling holes 311A on the corresponding side of the housing 31, even after the cooling fan is removed, the fan cooling holes 311A can also be used as ventilation holes. Enter the casing 31 through the cooling holes of the fan to play a cooling role. At this time, it is also possible to control the rotational speed of the centrifugal module 2 to realize rapid fluid flow of the air inside the casing 31 to achieve a good heat dissipation effect.

In addition to the fan cooling holes 311A, the casing 31 of this embodiment is also provided with ventilation cooling holes 311B to further improve the cooling effect. The casing 31 of this embodiment is provided with a plurality of ventilation and heat dissipation holes 311B arranged side by side,

The nucleic acid extraction device of this embodiment also includes a control module 4 . As shown in FIG. 8 , the control module 4 includes a master controller 41 and two slave controllers 42 corresponding to the two mixing modules 3 . The master controller 41 and the slave controller 42 use conductive slip rings 43 to solve the electrical routing problem. As shown in FIG. 2 , the conductive slip ring 43 is disposed on the extension shaft 25 . Specifically, the conductive slip ring 43 includes a stator end and a rotor end, the stator end is fixed on the motor bracket 28 through a fixed bracket, the electrical signal line of the stator end is connected with the main controller 41, the rotor end is fixed with the extension shaft 25, and the rotor end The electrical signal line is connected to the slave controller 42, so that the master controller and the slave controller are electrically connected, and power current and signal transmission can be realized. There are brushes and bearing structures inside the conductive slip ring, and the conductive slip ring keeps sliding contact with the brush when rotating to transmit power current and signals. The structure of the conductive slip ring is made of high temperature alloy and composite material, and no cooling is required during use.

The centrifugal module 2 is controlled by the master controller 41, the mixing module 3 and the heating module 5 are controlled by the slave controller 42, and the two slave controllers 42 are respectively fixed on both sides of the centrifugal tray 21 to control the functional modules on both sides respectively. The main controller 41 is connected with multiple groups of buttons and indicator lights, and the buttons are used to issue commands to the main controller 41. After the main controller 41 judges the order, it communicates with the slave controller 422 and performs corresponding operations. The main controller 41 controls and instructs The lights reflect the operating status of the unit. The user can use the buttons to operate the device, and read out the operating status of the device from the indicator light.

The working process of the nucleic acid extraction device of the present embodiment is as follows:

Put the nucleic acid reagent into the test tube A, put the test tube A into the test tube receiving hole 321 on the test tube fixing member 32, and lock it with the test tube fastening strip 39. During the mixing process, the output shaft of the stepping motor 35 makes a periodic circular motion, which drives the housing 31 to rotate. At this time, the test tube A placed in the housing 31 moves upside down, and the nucleic acid reagent inside the test tube A is also upside down. Make the nucleic acid reagent inside test tube A achieve the purpose of inverting and mixing. By controlling the heating of the electrothermal film, the reagent inside the test tube A is heated. The heating control can be performed simultaneously with the mixing control, that is, when the mixing module 3 performs mixing work, the test tube A moves upside down, and the heating module 5 simultaneously controls the heating of the reagents.

After the mixing and heating are completed, the reagent needs to be centrifugally separated. Under the state that the left and right sides of the mixing module 3 are guaranteed to be symmetrical, the centrifugal module 2 performs a centrifugal operation, and the mixing module 3 and the heating module 5 installed on the centrifugal tray 21 are driven. Centrifuge together to separate the reagents in test tube A under the action of centrifugal force.

The heating module 5 of this embodiment is installed in the mixing module 3, both of which are installed on the centrifuge module 2, so that the reagents can be mixed upside down along a certain angle on the slope while accompanied by heating control, and can also perform high-speed circular motion along the centrifugal axis . At the same time, the test tube A is placed at a certain angle, so that the reagents can be separated under the action of centrifugal force. The nucleic acid extraction device of this embodiment integrates three functional modules on one platform, and selects a combination of different functions according to the requirements of the nucleic acid extraction process, and each module cooperates to complete the automatic nucleic acid extraction process. Among them, the mixing module and the heating module are protected by a metal shell to prevent personnel from being injured by misoperation during high-temperature heating and high-speed centrifugation.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them; although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: the present invention can still be The specific implementation mode of the invention is modified or some technical features are equivalently replaced; without departing from the spirit of the technical solution of the present invention, all of them shall be included in the scope of the technical solution claimed in the present invention.

Claims (13)

1. A nucleic acid extraction device, comprising:

a base (1);

the centrifugal module (2) comprises a centrifugal tray (21) and a centrifugal driving device for driving the centrifugal tray (21) to rotate, and the centrifugal tray (21) is rotatably arranged on the base (1) around a centrifugal axis; and

mixing module (3), including test tube holder (32), test tube holder (32) have be used for holding test tube (A) test tube accommodation hole (321), mixing module (3) under drive arrangement's drive for centrifugal tray (21) rotate around mixing axis circumference in order to realize mixing to reagent, just test tube holder (32) around mixing axis rotationally set up in centrifugal tray (21), mixing axis for centrifugal axis slope sets up, test tube accommodation hole (321) the axis with mixing axis is crossed, nucleic acid extraction device still includes heating module (5), heating module (5) include heating piece, heating piece pair test tube holder (32) heat, mixing module (3) still including set up in casing (31) in the outside of test tube holder (32), casing (31) include cell body (311) and rotate connect in lid (312) on cell body (311), be provided with fastening strip (39) on the inner wall of lid (312) for centrifugal axis slope sets up, test tube holder (321) have the test tube holder (39) and hold down in the test tube holder (32) in the test tube holder (311) position when holding down test tube holder (39), the test tube fastening strip (39) is provided with a limiting groove, and the limiting groove extends along the distribution direction of the test tube accommodating holes (321) so that the test tube cover is clamped in the limiting groove.

2. The nucleic acid extraction device of claim 1, wherein the mixing axis is inclined at an angle in the range of 15 ° to 45 ° relative to the centrifugal axis.

3. The nucleic acid extraction device according to claim 1, characterized in that the axis of the cuvette receiving hole (321) is perpendicular to the mixing axis.

4. The nucleic acid extraction device according to claim 1, characterized in that the cuvette holder (32) is configured to be periodically positively and negatively rotated with respect to the centrifugation tray (21).

5. The nucleic acid extraction device according to claim 1, characterized in that it comprises two mixing modules (3) arranged symmetrically with respect to the centrifugal axis.

6. The nucleic acid isolation apparatus according to claim 5, wherein the centrifugal tray (21) includes a central tray body (211) and inclined tray bodies (212) provided on both sides of the central tray body (211), and the two mixing modules (3) are respectively provided on the two inclined tray bodies (212) in correspondence.

7. The nucleic acid isolation apparatus according to claim 1, wherein an elastic material is installed in the limit groove.

8. The nucleic acid extraction apparatus according to claim 1, characterized in that the cuvette holder (32) includes a connection side (322) on both sides of the bottom, the mixing module (3) further includes a spacer (33) provided between the connection side (322) and the bottom surface of the housing (31), and the connection side (322) is connected to the housing (31) through the spacer (33).

9. The nucleic acid isolation apparatus according to claim 1, wherein the heating member is an electrothermal film, and the electrothermal film is attached to an end surface of the cuvette holder (32).

10. The nucleic acid extraction apparatus according to claim 1, characterized in that the heating module (5) further comprises a temperature sensor, and the cuvette holder (32) further comprises a detection hole provided between two adjacent cuvette receiving holes (321), the temperature sensor being placed in the detection hole.

11. The nucleic acid extraction apparatus according to any one of claims 1 to 10, further comprising a heat radiation module (6), wherein the heat radiation module (6) comprises a side heat radiation fan (61) provided to a side of the cuvette holder (32); and/or, the heat dissipation module (6) further comprises a bottom surface heat dissipation fan (62) arranged on the bottom surface of the test tube fixing piece (32).

12. The nucleic acid extraction apparatus according to claim 11, characterized in that the mixing module (3) comprises a housing (31), and a fan heat radiation hole is provided in the housing (31).

13. The nucleic acid extraction device according to claim 1, further comprising a control module (4), the control module (4) comprising a main controller (41) coupled to the centrifugation module (2), a secondary controller (42) coupled to the mixing module (3), and an electrically conductive slip ring (43), the electrically conductive slip ring (43) being connected to the centrifugation tray (21) and electrically connecting the main controller (41) and the secondary controller (42).

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