CN221122574U

CN221122574U - Refrigerating module and refrigerating device

Info

Publication number: CN221122574U
Application number: CN202322959904.4U
Authority: CN
Inventors: 杨一杰; 张兴良; 臧天阳; 李春峰
Original assignee: Shenzhen Wellsim Biomedical Technologies
Current assignee: Shenzhen Wellsim Biomedical Technologies
Priority date: 2023-10-31
Filing date: 2023-10-31
Publication date: 2024-06-11
Anticipated expiration: 2033-10-31

Abstract

The application relates to the technical field of exosome separation, and particularly provides a refrigeration module and a refrigeration device. The refrigeration module comprises a storage box, a refrigeration assembly and a liquid level detection assembly. The storage box is provided with a first storage space and a second storage space. The first storage space is configured to be able to store a container for storing a biological sample. The second storage space is communicated with the first storage space and allows light rays to pass through. The refrigeration assembly is in heat-conducting contact with the storage box. The refrigeration assembly is configured to provide a first temperature. The liquid level detection assembly comprises an assembly box, an optical coupler and a heating piece. The assembly box is arranged in the second storage space. The optocoupler is arranged in the assembly box and is in heat conduction contact with the assembly box. The optocoupler is configured to detect a liquid level in the container by emitting light and receiving light. The optocoupler faces the first storage space. The heating element is arranged on the assembly box and is in heat conduction contact with the assembly box. The heating element is configured to provide a second temperature. The second temperature is greater than the first temperature.

Description

Refrigerating module and refrigerating device

Technical Field

The application relates to the technical field of exosome separation, in particular to a refrigeration module and a refrigeration device.

Background

Exosomes (exosomes) are vesicles of double phospholipid membrane structure secreted by cells and having a diameter of 30-150nm, widely and stably present in a variety of clinical samples, including blood, urine, ascites, interstitial fluid, tears, saliva, cerebrospinal fluid and the like. Exosomes serve as carriers for communication between cells, carry specific components derived from blast cells, such as proteins, nucleic acids, metabolic small molecules, and the like, and participate in a number of important physiological and pathological processes, including various events of tumor development, such as immune escape, angiogenesis, tumor metastasis, tumor resistance, and the like. The exosomes can be released by cancer cells earlier and continuously and enter the blood circulation system of a patient, and the lipid bilayer membrane structure can effectively protect carried proteins and encapsulated nucleic acid substances. Therefore, the exosomes are considered as key research objects in the fields of in-vitro diagnosis research and clinical detection of tumors, and are expected to play a great clinical value in the aspects of early diagnosis of tumors, evaluation of tumor metastasis and recurrence, evaluation of tumor heterogeneity, dynamic detection of tumorigenesis and development and curative effect, detection of drug-resistant mutation, personalized medicine application and the like.

The related method and apparatus for separating the fluid intimal vesicles such as cell culture fluid are limited by the structure of the separation chip itself for separation and purification, and the exosomes of the single separation and purification treatment of the separation chip have an upper limit, so that large-volume samples, such as samples with volumes of more than 10L, cannot be satisfied. Therefore, the liquid after single separation and purification of a single separation chip needs to be transferred and temporarily stored, but the storage temperature of an exosome has special requirements, if the liquid needing to be transferred and temporarily stored is placed in a refrigerator, on one hand, the transfer and the transportation of the liquid are inconvenient, and on the other hand, the volume of the liquid needing to be transferred and temporarily stored is inconvenient to observe, so that the automatic operation is inconvenient, and the efficiency requirements of the separation and purification operation of a large-volume sample cannot be met.

Disclosure of utility model

In view of the above, the present application provides a refrigeration module and a refrigeration device to satisfy separation and purification of exosomes of a large-volume sample.

An embodiment of the present application provides a refrigeration module. The refrigeration module comprises a storage box, a refrigeration assembly and a liquid level detection assembly. The storage box is provided with a first storage space and a second storage space. The first storage space is configured to be able to store a container for storing a biological sample. The second storage space is communicated with the first storage space and allows light rays to pass through. The refrigeration assembly is in heat-conducting contact with the storage box. The refrigeration assembly is configured to provide a first temperature. The liquid level detection assembly comprises an assembly box, an optical coupler and a heating piece. The assembly box is arranged in the second storage space. The optocoupler is arranged in the assembly box and is in heat conduction contact with the assembly box. The optocoupler is configured to detect a liquid level in the container by emitting light and receiving light. The optocoupler faces the first storage space. The heating element is arranged on the assembly box and is in heat conduction contact with the assembly box. The heating element is configured to provide a second temperature. The second temperature is greater than the first temperature.

In the above embodiment, the refrigeration assembly provides the first temperature and is in heat-conducting contact with the storage box, so that the temperature of the storage box is changed, and the temperature of the first storage space meets the storage requirement of the biological sample. The container storing biological samples is placed in the first storage space, so that the temperature requirement of temporary storage of biological samples can be met, and meanwhile, the container is placed or directly filled and taken by utilizing the structural form of the storage box. The optocoupler can be fixed in the second storage space through the assembly box, and the optocoupler can detect the liquid level of liquid in the container of the first storage space through judging the state of receiving light after emitting light towards the first storage space, so that the volume of the liquid is known according to the volume of the container, and the automatic filling of the liquid is convenient. And the second temperature that the heating piece provided carries out heat transfer through the assembly box, makes the temperature of opto-coupler be higher than the temperature in the first parking space, avoids opto-coupler surface to appear the comdenstion water and influences the light coupling and detect the accuracy of liquid level to the transfer of liquid is kept in the automation of being convenient for. The refrigeration module can be used for the treatment operation of separating and purifying the large-volume sample liquid.

In some embodiments of the application, a storage case includes a case body and a cover body. The box body is provided with a first storage space and a second storage space. The cover body is detachably connected with the box body. When the cover body is connected to the box body, the cover body can be covered in the first storage space to stop the container from moving out of the first storage space.

In the above embodiment, after the container is placed in the first storage space by using the disassembly and assembly cooperation of the box body and the cover body, the cover of the cover body can enable the first storage space to be relatively closed, so as to improve the stability of the temperature in the first storage space. Meanwhile, the cover body can also restrict the position of the inner container in the first storage space, so that the optical coupling is convenient to detect the liquid level.

In some embodiments of the application, the cover is provided with a communication port. The communication port communicates the first storage space with the outside of the storage box. The case further includes a first seal. The first sealing piece is arranged at the communication port. And the first seal is configured to be capable of sealing engagement with both the cover and the container.

In the above embodiment, the container can be connected with the external pipeline by extending out of the first storage space through the communication port portion, or the external pipeline can be connected with the container by extending into the first storage space through the communication port portion, so that the pipeline connection between the container and the external device is realized, and the automatic filling or the liquid extraction into the container is facilitated. And the first sealing piece can separate the space among the container, the box body and the cover body from the outside of the storage box, so that the generation of condensed water is reduced, and the stability of the temperature in the first storage space is facilitated to be maintained.

In some embodiments of the application, the cover includes a securing portion and a thermal insulation portion. The fixing part is connected with the box body. The fixed portion is configured to constrain a position of the container relative to the first storage space. The heat preservation portion is connected to one side of the fixing portion, which is away from the box body. The communication port extends from the fixing portion to the heat preservation portion. The first seal is provided to the insulating portion or is configured to be connected to the container.

In the above embodiment, the fixing portion is connected to the case and fixed relative to the case, and the fixing portion constrains the position of the container so that the container is fixed relative to the first storage space, which is beneficial to observing the liquid level in the container on one hand, and to connecting the container with an external pipeline on the other hand. Simultaneously, the heat preservation part and the first sealing piece can obstruct the first storage space to carry out heat transfer with the outside of the storage box through the fixing part, and the stability of the temperature in the first storage space is improved.

In some embodiments of the application, the refrigeration assembly is disposed outside of the cassette relative to the first storage space. The case also includes a second seal. The second sealing member is arranged on the outer side of the box body relative to the first storage space. The box body and the refrigeration component are respectively in sealing fit with the second sealing piece, and a sealing area is formed by surrounding.

In the above embodiment, if the refrigeration component is disposed in the box body, the fit between the assembly of the refrigeration component and the heat insulation performance of the first storage space needs to be considered. The refrigerating assembly is arranged on the outer side of the box body, so that the refrigerating assembly is convenient to assemble, the structure is simplified, and the design cost is reduced. Meanwhile, a sealing area is formed by arranging the second sealing piece, so that the formation of condensed water between the box body and the refrigerating assembly is reduced, the stability of the refrigerating assembly to the temperature regulation of the storage box is improved, heat transfer between the refrigerating assembly and the storage box is conducted through the sealing area as intensively as possible, and the efficiency of the refrigerating assembly to the temperature regulation of the storage box is improved.

In some embodiments of the application, the cartridge is provided with a drain channel. The liquid discharge channel is communicated with the first storage space and the outside of the storage box.

In the above embodiment, the box body can drain the condensed water in the first storage space out of the first storage space through the liquid drain channel, so as to avoid pollution or influence on the preservation of the biological sample in the first storage space.

In some embodiments of the present application, a refrigeration assembly includes a mount, a refrigeration member, a ventilation tube, a heat sink, and a fan. The fixing frame is arranged on the storage box. The refrigerating piece is arranged on the fixing frame. The refrigeration piece is in heat conduction contact with the storage box. The refrigeration member is configured to provide a first temperature. The ventilation pipe is connected with the fixing frame. The heat sink is at least partially located within the ventilation tube. The refrigerating element passes through the ventilation pipe or the radiating element passes through the ventilation pipe to enable the refrigerating element to be in heat conduction contact with the radiating element. The fan is arranged on the ventilation pipe. The fan is configured to create an airflow within the ventilation tube through the heat sink.

In the above embodiment, the refrigerating member is directly in heat-conducting contact with the storage box and provides the first temperature, thereby realizing the adjustment of the temperature of the storage box and improving the heat transfer efficiency. The refrigerating piece can be fixedly assembled in the storage box through the fixing frame, so that the stability of the position of the refrigerating piece is improved, and the temperature regulation efficiency of the refrigerating assembly to the storage box is improved. The heat generated by the operation of the refrigerating piece is transferred to the radiating piece and dissipated, and meanwhile, the fan is matched with the ventilation pipe to form stable air flow passing through the radiating piece, so that the heat dissipating efficiency of the radiating piece is improved, and the stability of the operation of the refrigerating piece for providing the first temperature is improved.

In some embodiments of the application, the refrigeration module further comprises a temperature sensing element. The temperature detection piece is arranged on the storage box and is in heat conduction contact with the storage box. The temperature detection piece is connected with the refrigerating piece through signals. The temperature detection piece is configured to, when the temperature detection piece detects that the storage box is higher than a set temperature, the refrigeration piece performs refrigeration operation, the set temperature is greater than or equal to the first temperature, and the set temperature is less than the second temperature.

In the above embodiment, the temperature detecting member detects the temperature of the storage box to determine whether the temperature in the first storage space can maintain the storage of the biological sample in the container, and when the requirement of the biological sample storage temperature is not met, the refrigerating member is started in time to perform the operation, so as to avoid the damage of the biological sample. And meanwhile, if the temperature of the storage box reaches the set temperature, the refrigerating piece is allowed to suspend operation, so that the energy consumption is reduced.

In some embodiments of the application, the mounting box includes a thermally conductive member and a thermally insulating member. The optocoupler and the heating piece are both arranged on the heat conducting piece and are both in heat conducting contact with the heat conducting piece. The heat conducting piece is configured to be coated on the outer side of the optical coupler and extends to the light emitting side and the light receiving side of the optical coupler. The heat insulating piece is coated on the outer sides of the heating piece and the heat conducting piece. The heat insulating piece is connected with the assembly box.

In the above embodiment, the second temperature provided by the heating element is transferred to the optocoupler through the heat conducting element, so that the temperature of each part of the optocoupler is uniformly adjusted, the possibility that condensed water is generated on the light emitting side and the light receiving side of the optocoupler is reduced, and the accuracy of the optocoupler on liquid level detection is improved. Meanwhile, the heat insulation piece is coated on the outer sides of the optocoupler, the heating piece and the heat conduction piece, so that the temperature in the second storage space is prevented from being influenced by the temperatures of the optocoupler, the heating piece and the heat conduction piece, the temperature in the first storage space is prevented from being influenced, and the stability of the storage temperature of the biological sample is improved.

An embodiment of the present application provides a refrigeration device. The refrigeration device comprises a container and a refrigeration module as described in any of the embodiments above. The container is configured to hold a biological sample. The container is placed in the first storage space.

In the above embodiments, the container is placed in the first storage space of the storage box, and the refrigeration assembly provides the first temperature to the storage box, thereby maintaining the container at the temperature required for storage of the biological sample. At the same time the storage case can hold the container for easy filling or drawing of liquid from the container. The light emitted by the optical coupler and the received light pass through the first storage space and the second storage space, so that the detection of the liquid level in the container is realized, and the container is automatically filled or pumped with liquid. The liquid level detection assembly is arranged in the second storage space, so that the heating element can heat the optocoupler through the assembly box, condensed water is prevented from being generated on the surface of the optocoupler, meanwhile, the influence on the temperature in the first storage space is reduced, and the stability of the storage temperature of the biological sample is maintained. Therefore, the refrigerating device can be used in the treatment operation process of separating and purifying the large-volume sample liquid.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following description will briefly describe the drawings in the embodiments, it being understood that the following drawings only illustrate some embodiments of the present application and should not be considered as limiting the scope.

FIG. 1 is an exploded view of a refrigeration apparatus according to an embodiment of the present application;

FIG. 2 is a schematic diagram of the assembled refrigeration device of FIG. 1 with the insulation jacket omitted;

FIG. 3 is a schematic cross-sectional view of the fluid level detection assembly of FIG. 1.

Description of main reference numerals:

Refrigeration module 100

Storage case 10

Box body 101

First storage space 1011

Second storage space 1012

Cover 102

Communication port 1021

Fixing part 1022

Thermal insulation part 1023

First seal 103

Insulation cover 104

Refrigeration assembly 20

Fixing frame 201

Refrigeration member 202

Vent 203

Radiator 204

Fan 205

Temperature detecting member 206

Temperature control switch 207

Liquid level detection assembly 30

Assembly box 301

Heat conductive member 3011

Thermal insulation 3012

Groove 3012a

Optocoupler 302

Heating element 303

Refrigerating apparatus 200

Container 210

Detailed Description

The following description of the technical solutions according to the embodiments of the present application will be given with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are only some embodiments of the present application, but not all embodiments.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

The terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. The symbol "-" is understood to include its endpoints.

The embodiment of the application provides a refrigeration module and a refrigeration device. The refrigeration module is used for the refrigeration device. The refrigeration module comprises a storage box, a refrigeration assembly and a liquid level detection assembly. The storage box is provided with a first storage space and a second storage space. The first storage space is configured to be able to store a container for storing a biological sample. The second storage space is communicated with the first storage space and allows light rays to pass through. The refrigeration assembly is in heat-conducting contact with the storage box. The refrigeration assembly is configured to provide a first temperature. The liquid level detection assembly comprises an assembly box, an optical coupler and a heating piece. The assembly box is arranged in the second storage space. The optocoupler is arranged in the assembly box and is in heat conduction contact with the assembly box. The optocoupler is configured to detect a liquid level in the container by emitting light and receiving light. The optocoupler faces the first storage space. The heating element is arranged on the assembly box and is in heat conduction contact with the assembly box. The heating element is configured to provide a second temperature. The second temperature is greater than the first temperature.

The refrigeration assembly provides a first temperature and is in heat-conducting contact with the storage box, so that the temperature of the storage box is changed, and the temperature of the first storage space meets the storage requirement of the biological sample. The container storing biological samples is placed in the first storage space, so that the temperature requirement of temporary storage of biological samples can be met, and meanwhile, the container is placed or directly filled and taken by utilizing the structural form of the storage box. The optocoupler can be fixed in the second storage space through the assembly box, and the optocoupler can detect the liquid level of liquid in the container of the first storage space through judging the state of receiving light after emitting light towards the first storage space, so that the volume of the liquid is known according to the volume of the container, and the automatic filling of the liquid is convenient. And the second temperature that the heating piece provided carries out heat transfer through the assembly box, makes the temperature of opto-coupler be higher than the temperature in the first parking space, avoids opto-coupler surface to appear the comdenstion water and influences the light coupling and detect the accuracy of liquid level to the transfer of liquid is kept in the automation of being convenient for. The refrigeration module can be used for the treatment operation of separating and purifying the large-volume sample liquid.

Some embodiments of the present application are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without collision.

Referring to fig. 1 and 2, an embodiment of the present application provides a refrigeration module 100 and a refrigeration apparatus 200. The refrigeration module 100 and the refrigeration device 200 are used for transit temporary storage of a liquid containing a biological sample in a biological sample separation device. It is understood that the liquid containing the biological sample may be, by way of illustrative example, a cell culture liquid, a bacterial culture liquid, human plasma, serum, cerebral spinal fluid, saliva, urine, gastric fluid, etc.; biological samples include, but are not limited to, proteins, cells, bacteria, and membranous vesicles such as exosomes or circulating tumor cells. In other embodiments, the liquid may be plants, animals other than humans, microorganisms, etc., and may even be a non-biological sample. The liquid containing the biological sample that the refrigeration module 100 can store includes a liquid prepared before treatment or a liquid temporarily stored during treatment or a liquid collected after treatment when the biological sample is separated and purified.

Referring to fig. 1 and 2, in some embodiments, a refrigeration module 100 includes a storage cassette 10, a refrigeration assembly 20, and a liquid level detection assembly 30. The storage box 10 is provided with a first storage space 1011 and a second storage space 1012. The first storage space 1011 is configured to be capable of storing a container 210 for storing a biological sample. The second storage space 1012 communicates with the first storage space 1011 and allows light to pass through. The refrigeration assembly 20 is in thermally conductive contact with the case 10. The refrigeration assembly 20 is configured to provide a first temperature. The fluid level detection assembly 30 includes a mounting box 301, an optocoupler 302, and a heating element 303, as shown in FIG. 3. The set up cassette 301 is provided in the second storage space 1012. The optocoupler 302 is provided in the assembly box 301 and is in heat-conductive contact with the assembly box 301. Optocoupler 302 is configured to detect the level of liquid within container 210 by emitting light and receiving light. The optocoupler 302 faces the first storage space 1011. The heating element 303 is provided in the assembly box 301 and is in heat-conducting contact with the assembly box 301. The heating element 303 is configured to provide a second temperature. The second temperature is greater than the first temperature.

The refrigeration assembly 20 provides a first temperature and is in thermally conductive contact with the case 10, thereby altering the temperature of the case 10 itself such that the temperature of the first storage space 1011 meets the storage requirements of the biological sample. The container 210 storing the biological sample is placed in the first storage space 1011, which can meet the temperature requirement of temporary storage of the biological sample, and meanwhile, the structural form of the storage box 10 is also beneficial to placing and taking the container 210 or directly filling and taking the container 210. The optocoupler 302 can be fixed in the second storage space 1012 through the assembly box 301, and after the optocoupler 302 emits light towards the first storage space 1011, the liquid level of the liquid in the container 210 of the first storage space 1011 can be detected through judging the state of receiving the light, so that the volume of the liquid can be known according to the volume of the container 210, and the automatic filling and the extraction of the liquid are convenient. And the second temperature provided by the heating element 303 is transferred through the assembly box 301, so that the temperature of the optocoupler 302 is higher than the temperature in the first storage space 1011, and the influence of condensed water on the surface of the optocoupler 302 on the accuracy of detecting the liquid level by the optocoupler 302 is avoided, thereby facilitating the automatic transfer and temporary storage of the liquid. The refrigeration module 100 can be used for processing operations for separation and purification of large volumes of sample liquid.

It will be appreciated that in some embodiments, the storage temperature is required to maintain the activity of the biological sample during storage, and as an exemplary example, the storage temperature of the exosome needs to be maintained at 4±2 ℃, whereas the biological sample separation device typically operates at room temperature (typically 25 ℃), so that the first temperature provided by the cooling element 20 cannot be higher than the storage temperature of the biological sample, and the second temperature provided by the heating element 303 is typically higher than the room temperature, for example, the heating element 303 can heat the surface of the optocoupler 302 to about 40-60 ℃ after providing the second temperature, and in a practical working environment, the cooling element 20 and the heating element 303 take into account the loss caused by energy transfer via different media during temperature adjustment.

Referring to fig. 1 and 2, in some embodiments, the first storage space 1011 is provided with a plurality of. Each first storage space 1011 stores one container 210, and different liquids can be stored in the containers 210 of different first storage spaces 1011. The second storage spaces 1012 are provided in plurality, and each first storage space 1011 is respectively communicated with one or more second storage spaces 1012, so that each first storage space 1011 can perform liquid level detection through one or more optocouplers 302. It will be appreciated that in some embodiments, each first storage space 1011 and each second storage space 1012 can be in communication with the exterior of the case 10, respectively, to facilitate the independent assembly of the container 210 and the assembly of the liquid level detection assembly 30, respectively.

Referring to fig. 1 and 2, in some embodiments, the case 10 includes a case 101 and a cover 102. The cassette 101 is provided with a first storage space 1011 and a second storage space 1012. The cover 102 is detachably connected to the case 101. When the cover 102 is connected to the box 101, the cover 102 can cover the first storage space 1011 to stop the container 210 from moving out of the first storage space 1011.

After the container 210 is placed in the first storage space 1011 by means of the dismounting and mounting cooperation of the box body 101 and the cover body 102, the cover of the cover body 102 can enable the first storage space 1011 to be relatively closed, so that the stability of the temperature in the first storage space 1011 is improved. At the same time, the cover 102 can also restrict the position of the container 210 in the first storage space 1011, so that the optocoupler 302 can detect the liquid level.

It is appreciated that in some embodiments, the first storage space 1011 may extend to the cover 102.

Referring to fig. 1, in some embodiments, the cover 102 is provided with a communication port 1021. The communication port 1021 communicates the first storage space 1011 with the outside of the storage box 10. The case 10 further includes a first seal 103. The first seal 103 is provided at the communication port 1021. And the first seal 103 is configured to be capable of sealing engagement with both the cover 102 and the container 210. As an exemplary example, the first seal 103 includes, but is not limited to, a rubber ring, a rubber sleeve, and the like.

The container 210 can be connected with an external pipeline by partially extending out of the first storage space 1011 through the communication port 1021, or the external pipeline can be connected with the container 210 by extending into the first storage space 1011 through the communication port 1021, thereby realizing the pipeline connection of the container 210 and external equipment so as to facilitate the automatic filling or drawing of liquid into the container 210. And the first sealing member 103 may separate the space among the container 210, the case 101 and the cover 102 from the outside of the storage case 10, thereby reducing the generation of condensed water, and thus being beneficial to maintaining the stability of the temperature in the first storage space 1011.

It will be appreciated that in some embodiments, the first seal 103 is provided on the outside of the container lid of the container 210. In other embodiments, the first seal 103 may be omitted, and the container cover of the container 210 is configured to be capable of sealing engagement with the communication port 1021 of the case 10.

Referring to fig. 1 and 2, in some embodiments, the cover 102 includes a securing portion 1022 and a thermal insulation portion 1023. The fixing portion 1022 is connected to the case 101. The securing portion 1022 is configured to constrain the position of the container 210 relative to the first storage space 1011. The insulating portion 1023 is connected to a side of the fixing portion 1022 facing away from the case 101. The communication port 1021 extends from the fixing portion 1022 to the heat insulating portion 1023. The first seal 103 is provided in the heat insulating portion 1023.

The fixing portion 1022 is connected to the case 101 and is fixed relative to the case 101, and the fixing portion 1022 constrains the position of the container 210 so that the container 210 is fixed relative to the first storage space 1011, which is advantageous for observing the liquid level in the container 210 and for connecting the container 210 to an external pipeline. Meanwhile, the heat preservation part 1023 and the first sealing member 103 can block the first storage space 1011 from heat transfer with the outside of the storage box 10 through the fixing part 1022, so that the stability of the temperature in the first storage space 1011 is improved.

Referring to fig. 1 and 2, in some embodiments, insulating portion 1023 is a sheet-like structure. It can be understood that the heat preservation portion 1023 is formed by splicing a plurality of sheet structures, and the plurality of sheet structures are clamped in the container 210 when spliced, so as to reduce the gap between the containers 210 at the connection port 1021 of the heat preservation portion 1023 and improve the heat preservation performance.

Referring to fig. 1 and 2, in some embodiments, the refrigeration assembly 20 is disposed outside of the cassette 101 relative to the first storage space 1011. The case 10 also includes a second seal (not shown). The second seal is provided outside the cartridge 101 with respect to the first storage space 1011. The box body 101 and the refrigeration assembly 20 are respectively in sealing fit with the second sealing piece, and enclose to form a sealing area.

If the refrigeration unit 20 is disposed in the case 101, the fit between the assembly of the refrigeration unit 20 and the thermal insulation performance of the first storage space 1011 needs to be considered. The refrigeration assembly 20 is arranged on the outer side of the box body 101, so that the refrigeration assembly 20 is convenient to assemble, the structure is simplified, and the design cost is reduced. Meanwhile, a sealing area is formed by arranging the second sealing piece, so that the formation of condensed water between the box body 101 and the refrigerating assembly 20 is reduced, the stability of the refrigerating assembly 20 on the temperature regulation of the storage box 10 is improved, heat transfer between the refrigerating assembly 20 and the storage box 10 is conducted through the sealing area as intensively as possible, and the efficiency of the refrigerating assembly 20 on the temperature regulation of the storage box 10 is improved.

It is understood that in some embodiments, the second seal includes, but is not limited to being, a waterproof glue or the like. On the one hand, the sealing function can be achieved, and on the other hand, the stability of the connection between the refrigeration assembly 20 and the box body 101 can be improved.

In some embodiments, the cassette 101 is provided with a drain channel (not shown). The drain passage communicates the first storage space 1011 with the outside of the storage box 10. The box 101 can drain the condensed water in the first storage space 1011 out of the first storage space 1011 through the liquid drain channel, thereby avoiding pollution or affecting the preservation of the biological sample in the first storage space 1011.

In some embodiments, the drain passage is located at the bottom of the first storage space 1011 in communication with the first storage space 1011. The drain passage is provided as an inclined surface or a groove body so that condensed water is automatically discharged from the first storage space 1011 under the action of gravity. In some embodiments, the plurality of first storage spaces 1011 are mutually communicated, the liquid discharge channel is provided with one liquid discharge channel and is communicated with one first storage space 1011, and condensed water in the plurality of first storage spaces 1011 flows into the first storage space 1011 provided with the liquid discharge channel and then intensively flows out from one liquid discharge channel, so that the stability of the temperature in the first storage space 1011 is improved.

Referring to fig. 1, in some embodiments, the case 10 further includes a thermal sleeve 104. The heat insulation sleeve 104 is sleeved on the outer side of the box body 101 and the outer side of the cover body 102 at the same time, and covers the joint of the box body 101 and the cover body 102, so as to isolate heat transfer between the box body 101 and the outside of the storage box 10 as far as possible, thereby maintaining the stability of the temperature in the first storage space 1011.

Referring to fig. 1 and 2, in some embodiments, the refrigeration assembly 20 includes a mount 201, a refrigeration member 202, a vent tube 203, a heat sink 204, and a fan 205. The holder 201 is provided in the case 10. The refrigerating element 202 is provided in the holder 201. The refrigeration member 202 is in thermally conductive contact with the case 10. The refrigeration member 202 is configured to provide a first temperature. The ventilation tube 203 is connected to the holder 201. The heat sink 204 is at least partially located within the vent tube 203. The cooling element 202 passes through the vent tube 203 or the heat sink 204 passes through the vent tube 203 bringing the cooling element 202 into heat conductive contact with the heat sink 204. A fan 205 is provided in the ventilation duct 203. The fan 205 is configured to create an airflow within the ventilation tube 203 through the heat sink 204.

The cooling element 202 is in direct heat-conducting contact with the case 10 and provides a first temperature, thereby effecting temperature regulation of the case 10 and improving heat transfer efficiency. The refrigerating piece 202 can be fixedly assembled to the storage box 10 through the fixing frame 201, so that the stability of the position of the refrigerating piece 202 is improved, and the efficiency of the refrigerating assembly 20 for adjusting the temperature of the storage box 10 is improved. The heat generated by the operation of the refrigerating element 202 is transferred to the heat radiating element 204 and dissipated, and meanwhile, the fan 205 is matched with the ventilation pipe 203 to form stable air flow passing through the heat radiating element 204, so that the heat dissipating efficiency of the heat radiating element 204 is improved, and the stability of the operation of the refrigerating element 202 for providing the first temperature is improved. It will be appreciated that in some embodiments, the second seal is sealingly connected to the mount 201.

Referring to fig. 1, in some embodiments, the cooling member 202 is a semiconductor cooling fin and the heat sink 204 is a heat sink fin. The gaps of the radiating fins are parallel to the extending direction of the vent pipe 203, and along the radial direction of the vent pipe 203, the extending length of the radiating fins approaches to the radial direction of the vent pipe 203, so that when the air flows in the vent pipe 203, the air flow in the radial direction of the vent pipe 203 can pass through the radiating fins, and the radiating efficiency of the radiating fins is improved.

Referring to fig. 1 and 2, in some embodiments, the vent tube 203 extends in a straight line. Two fans 205 are arranged at two ends of the ventilation pipe 203, and the fans 205 at the two ends face the same direction to form unidirectional wind in the ventilation pipe 203, so that the heat dissipation efficiency of the heat dissipation piece 204 is improved.

Referring to fig. 1, in some embodiments, the refrigeration module 100 further includes a temperature detector 206 and a temperature control switch 207. The temperature detecting member 206 and the temperature control switch 207 are respectively connected with the refrigerating member 202 in a signal manner.

The temperature detecting member 206 is provided to the storage case 10 and is in heat conductive contact with the storage case 10. The temperature detecting member 206 is configured such that when the temperature detecting member 206 detects that the storage box 10 is higher than a set temperature, the cooling member 202 performs a cooling operation, the set temperature being equal to or higher than the first temperature, and the set temperature being lower than the second temperature. It is appreciated that in some embodiments, the refrigeration module 100 also includes a controller (not shown). The controller receives the temperature information detected by the temperature detecting element 206, and sends a control command to the refrigerating element 202 according to the temperature information, so as to adjust the working power of the refrigerating element 202, thereby maintaining the temperature of the box 101 at the set temperature.

The temperature detecting member 206 detects the temperature of the storage box 10 to determine whether the temperature in the first storage space 1011 can maintain the storage of the biological sample in the container 210, and when the requirement of the biological sample storage temperature is not met, the refrigerating member 202 is started to operate in time, so as to avoid the damage of the biological sample. And at the same time, if the temperature of the storage box 10 reaches the set temperature, the refrigerating piece 202 is allowed to stop operation, so that the energy consumption is reduced.

The temperature control switch 207 is arranged on the surface of the heat dissipation element 204. The temperature control switch 207 is capable of monitoring the temperature of the refrigeration member 202. The temperature control switch 207 is configured such that when the temperature control switch 207 detects that the temperature of the cooling element 202 exceeds the protection temperature, the temperature control switch 207 controls the cooling element 202 to stop working, so as to avoid burning out the elements of the cooling element 202 due to over temperature, and also reduce the possibility of damaging other components due to damage of the cooling element 202.

It will be appreciated that in some embodiments, the change of the adaptability of the set temperature may enable the temperature in the first storage space 1011 to satisfy the storage temperature of the biological sample when the cartridge 101 is at the set temperature according to the biological sample stored in the first storage space 1011.

Referring to fig. 1-3, in some embodiments, the assembly box 301 includes a thermally conductive member 3011 and a thermally insulating member 3012. Optocoupler 302 and heating element 303 are both disposed on thermally conductive element 3011 and are both in thermally conductive contact with thermally conductive element 3011. The heat conductive member 3011 is configured to cover the outside of the optocoupler 302 and extend to the light emitting side and the light receiving side of the optocoupler 302. The heat insulating member 3012 is wrapped around the heating member 303 and the heat conducting member 3011. The insulation 3012 is connected to the mounting box 301.

The second temperature provided by the heating element 303 is transferred to the optocoupler 302 through the heat conducting element 3011, so that the temperature of the optocoupler 302 is uniformly adjusted all over, the possibility that condensed water is generated on the light emitting side and the light receiving side of the optocoupler 302 is reduced, and the accuracy of the optocoupler 302 on liquid level detection is improved. Meanwhile, the heat insulation piece 3012 is wrapped on the outer sides of the optocoupler 302, the heating piece 303 and the heat conduction piece 3011, so that the temperature of the optocoupler 302, the heating piece 303 and the heat conduction piece 3011 is prevented from affecting the temperature in the second storage space 1012, the temperature in the first storage space 1011 is prevented from being affected, and the stability of the storage temperature of the biological sample is improved.

Referring to fig. 3, in some embodiments, a groove 3012a is provided on the inner side of the heat shield 3012, so that a gap is formed between the heat shield 3012 and the heat conductive member 3011 to enhance the heat shielding effect of the heat shield 3012.

As an exemplary example, the heat conductive member 3011 may be made of, but not limited to, a material such as aluminum alloy, and may have good heat conductivity and good mechanical strength. The heat insulating material 3012 may be made of, but not limited to, bakelite (PF, phenolic plastic) or the like, and may have high mechanical strength, good insulation, and good thermal insulation.

It will be appreciated that in some embodiments, when the optocoupler 302 emits light and receives light, it is determined that the liquid level in the container 210 does not reach the corresponding height, and when the optocoupler 302 emits light but fails to receive light, it is determined that the liquid level in the container 210 reaches the corresponding height.

Referring to fig. 1 and 2, one embodiment of the present application provides a refrigeration apparatus 200. The refrigeration device 200 includes a container 210 and the refrigeration module 100 as described in any of the embodiments above. The container 210 is configured to be capable of holding a biological sample. The container 210 is placed in the first storage space 1011.

The container 210 is placed in the first storage space 1011 of the case 10 and the refrigeration assembly 20 provides a first temperature to the case 10, thereby maintaining the container 210 at a temperature required for storage of the biological sample. While the case 10 is capable of holding the container 210 to facilitate filling or drawing of the container 210 with liquid. The light emitted by the optocoupler 302 and the received light pass through the first storage space 1011 and the second storage space 1012, so as to realize detection of the liquid level in the container 210, so as to facilitate automatic filling or liquid extraction of the container 210. The liquid level detecting assembly 30 is disposed in the second storage space 1012, so that the heating element 303 can heat the optocoupler 302 through the assembly box 301, thereby avoiding the surface of the optocoupler 302 from generating condensed water, reducing the influence on the temperature in the first storage space 1011, and being beneficial to maintaining the stability of the storage temperature of the biological sample. The refrigeration device 200 can be used in the process of separating and purifying a large volume of sample liquid.

Referring to fig. 1 and 2, in some embodiments, the first storage space 1011 is provided in plurality, and different first storage spaces 1011 may be configured in different shapes such as square, circular, etc. in cross section to store containers 210 of different shapes. Different containers 210 are capable of holding different liquids, different containers 210 being, for example, pre-treatment cell sap bottles, primary purification intermediate transfer bottles, exosome bottles, etc.

In addition, those skilled in the art will recognize that the foregoing embodiments are merely illustrative of the present application and are not intended to be limiting, as appropriate modifications and variations of the foregoing embodiments are within the scope of the disclosure of the application.

Claims

1. A refrigeration module, characterized by: comprising the following steps:

A storage box provided with a first storage space and a second storage space, wherein the first storage space is configured to be capable of storing a container for storing a biological sample, and the second storage space is communicated with the first storage space and allows light to pass through;

a refrigeration assembly in thermally conductive contact with the storage case, the refrigeration assembly configured to be capable of providing a first temperature;

The liquid level detection assembly comprises an assembly box, an optical coupler and a heating piece, wherein the assembly box is arranged in the second storage space, the optical coupler is arranged in the assembly box and is in heat conduction contact with the assembly box, the optical coupler is configured to detect the liquid level in the container by emitting light rays and receiving light rays, the optical coupler faces the first storage space, the heating piece is arranged in the assembly box and is in heat conduction contact with the assembly box, and the heating piece is configured to provide a second temperature which is higher than the first temperature.

2. The refrigeration module of claim 1, wherein: the storage box comprises a box body and a cover body, the box body is provided with the first storage space and the second storage space, the cover body is detachably connected with the box body,

When the cover body is connected with the box body, the cover body can be covered in the first storage space to stop the container from moving out of the first storage space.

3. A refrigeration module as set forth in claim 2 wherein: the cover body is provided with a communication port, the communication port communicates the first storage space with the external world of case, the case still includes first sealing member, first sealing member locates the communication port department, just first sealing member is configured to can with the cover body and with the equal sealed laminating of container.

4. A refrigeration module as set forth in claim 3 wherein: the cover body comprises a fixing portion and a heat preservation portion, the fixing portion is connected with the box body, the fixing portion is configured to restrict the position of the container relative to the first storage space, the heat preservation portion is connected to one side, away from the box body, of the fixing portion, the communication port extends from the fixing portion to the heat preservation portion, and the first sealing piece is arranged on the heat preservation portion or is configured to be connected with the container.

5. A refrigeration module as set forth in claim 2 wherein: the refrigerating assembly is arranged on the outer side of the box body relative to the first storage space, the storage box further comprises a second sealing piece, the second sealing piece is arranged on the outer side of the box body relative to the first storage space, and the box body and the refrigerating assembly are respectively and tightly attached to the second sealing piece and enclose to form a sealing area.

6. A refrigeration module as set forth in claim 2 wherein: the box body is provided with a liquid discharge channel, and the liquid discharge channel is communicated with the first storage space and the outside of the storage box.

7. The refrigeration module of any one of claims 1 to 6, wherein: the refrigeration assembly comprises a fixing frame, a refrigeration piece, a ventilation pipe, a heat dissipation piece and a fan, wherein the fixing frame is arranged on the storage box, the refrigeration piece is arranged on the fixing frame, the refrigeration piece is in heat conduction contact with the storage box, the refrigeration piece is configured to be capable of providing a first temperature, the ventilation pipe is connected with the fixing frame, the heat dissipation piece is at least partially located in the ventilation pipe, the refrigeration piece penetrates through the ventilation pipe or the heat dissipation piece penetrates through the ventilation pipe to enable the refrigeration piece to be in heat conduction contact with the heat dissipation piece, the fan is arranged on the ventilation pipe, and the fan is configured to be capable of forming an airflow passing through the heat dissipation piece in the ventilation pipe.

8. The refrigeration module of claim 7, wherein: the refrigerating module further comprises a temperature detection part which is in signal connection with the refrigerating part, the temperature detection part is arranged on the storage box and is in heat conduction contact with the storage box, and the temperature detection part is configured to perform operation at a first temperature when the temperature detection part detects that the storage box is higher than a set temperature, the set temperature is higher than or equal to the first temperature, and the set temperature is lower than the second temperature.

9. The refrigeration module of any one of claims 1 to 6, wherein: the assembly box comprises a heat conducting piece and a heat insulating piece, wherein the light coupler and the heating piece are both arranged on the heat conducting piece and are in heat conducting contact with the heat conducting piece, the heat conducting piece is structured to be coated on the outer side of the light coupler and extends to the light emitting side and the light receiving side of the light coupler, the heat insulating piece is coated on the outer side of the heating piece and the heat conducting piece, and the heat insulating piece is connected with the assembly box.

10. A refrigeration device, characterized by: comprising a container configured to be able to store a biological sample, the container being placed in the first storage space, and a refrigeration module according to any of claims 1 to 9.