CN207002730U - A kind of quantitative fluorescent PCR system - Google Patents
A kind of quantitative fluorescent PCR system Download PDFInfo
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- CN207002730U CN207002730U CN201720693813.2U CN201720693813U CN207002730U CN 207002730 U CN207002730 U CN 207002730U CN 201720693813 U CN201720693813 U CN 201720693813U CN 207002730 U CN207002730 U CN 207002730U
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- heat
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- radiating block
- conducting seat
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Abstract
The utility model discloses a kind of quantitative fluorescent PCR system, including heat-conducting seat and fluorescence detector, array is placed with the tube seat for holding test tubes on heat-conducting seat, the top of heat-conducting seat is provided with sample cell bracket, sample cell bracket is provided with and the one-to-one circular hole of tube seat, the top of test tube is provided with capping plate, the both sides of capping plate are arranged with flange, the both sides of sample cell bracket are hinged with the clasp coordinated with flange, multiple electric heating pieces are embedded with heat-conducting seat, the bottom of heat-conducting seat is provided with radiating block, radiating block is provided with the through hole connected with tube seat, the both sides of radiating block are provided with heat pipe, the lower section of radiating block is provided with positioning screw rod, fluorescence detector is fixed on the sliding block of positioning screw rod.The utility model realizes the rapid increase temperature of heat-conducting seat by setting multiple electric heating pieces, and coordinates the fast cooling of radiating block and Heat Pipes heat-conducting seat, and fluorescence detector uses bottom type, by rationally controlling the thickness of radiating block to effectively improve accuracy of detection.
Description
Technical field
The utility model belongs to fluorescence analysis field, and in particular to a kind of quantitative fluorescent PCR system.
Background technology
PCR（PCR）The general principle of detecting system is similar to DNA natural reproduction process, its specificity
Dependent on the Oligonucleolide primers complementary with target sequence both ends, molecular biology research, clinical diagnosis are widely used at present
Deng in field.But existing PCR detection system mostly has the problem of heating and cooling process is slow, it is unfavorable for the sexually transmitted disease that happens suddenly
Detection, meanwhile, influenceed by the position of heating module, the mode of fluorescence detector generally use top or sidepiece detection, but detect
During disturbing factor it is more, such as lid steam, optical fiber fracture easy to wear, it is difficult to reach the stability and precision of detection
It is required that.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of simple to operate, and stability is good, and accuracy of detection is higher
Quantitative fluorescent PCR system.
In order to solve the above technical problems, the technical solution adopted in the utility model is：
A kind of quantitative fluorescent PCR system, including heat-conducting seat and fluorescence detector, array is placed with for placing on heat-conducting seat
The tube seat of test tube, the top of heat-conducting seat are provided with sample cell bracket, and sample cell bracket is provided with and the one-to-one circular hole of tube seat, circle
The diameter in hole is less than the external diameter of the test tube mouth of pipe, and the top of test tube is provided with capping plate, and the both sides for covering plate are arranged with flange, sample
The both sides of pond bracket are hinged with the clasp coordinated with flange, are embedded with multiple electric heating pieces in heat-conducting seat, the bottom of heat-conducting seat is provided with
Radiating block, radiating block are provided with the through hole connected with tube seat, and the both sides of radiating block are provided with heat pipe, and the lower section of radiating block is provided with positioning
Screw mandrel, fluorescence detector is multiple to arrange from inside to outside, and is each attached on the sliding block of positioning screw rod, and positioning screw rod passes through step
Stepper motor drives.
The capping plate includes at least one top cover, and the bottom of top cover is provided with diaphragm seal.
At least one layer of heating tape is provided between the sample cell bracket and heat-conducting seat.
Heat conductive silica gel is provided between the side wall of the tube seat and test tube, heat conductive silica gel is fixed on tube seat.
The diameter of the through hole is not less than the diameter of tube seat bottom.
The utility model has the advantages that：The utility model is by setting multiple electric heating pieces to realize the rapid increase of heat-conducting seat
Temperature, and coordinate the fast cooling of radiating block and Heat Pipes heat-conducting seat, be advantageous to the detection of burst sexually transmitted disease, it is simple to operate,
Stability is good, and fluorescence detector uses bottom type, by rationally controlling the thickness of radiating block to effectively improve accuracy of detection.
Brief description of the drawings
The utility model is described in further detail with reference to the accompanying drawings and detailed description.
Fig. 1 is structural representation of the present utility model.
Wherein, 1, test tube, 2, diaphragm seal, 21, top cover, 3, sample cell bracket, 4, heating tape, 5, heat-conducting seat, 51, tube seat,
52nd, heat conductive silica gel, 6, electric heating piece, 7, radiating block, 71, heat pipe, 72, through hole, 8, fluorescence detector, 9, positioning screw rod, 10, button
Ring, 11, flange.
Embodiment：
As shown in figure 1, a kind of quantitative fluorescent PCR system, including heat-conducting seat 5 and fluorescence detector 8, array on heat-conducting seat 5
Be placed with the tube seat 51 for holding test tubes 1, the top of heat-conducting seat 5 is provided with sample cell bracket 3, sample cell bracket 3 be provided with
51 one-to-one circular hole of tube seat, the diameter of circular hole are less than the external diameter of the mouth of pipe of test tube 1, so as to which test tube 1 is hung up in sample cell support
On frame 3, the top of test tube 1 is provided with capping plate, and the both sides for covering plate are arranged with flange 11, and the both sides of sample cell bracket 3 are hinged
There is the clasp 10 coordinated with flange 11, capping plate can both be fixed on the top of test tube 1, can pass through sample cell bracket again by clasp 10
3 batch transfer samples, heat-conducting seat 5 is interior to be embedded with multiple electric heating pieces 6, and the bottom of heat-conducting seat 5 is provided with radiating block 7, set on radiating block 7
There is the through hole 72 connected with tube seat 51, the both sides of radiating block 7 are provided with heat pipe 71, and the lower section of radiating block 7 is provided with positioning screw rod 9, glimmering
Photodetector 8 is multiple to arrange from inside to outside, and is each attached on the sliding block of positioning screw rod 9, and positioning screw rod 9 passes through stepping electricity
Machine drives.
In the utility model, electric heating piece 6, which is tried one's best, is distributed in the both sides of test tube 1, make heat-conducting seat 5 even heat pass
Pass.At least one layer of heating tape 4, temperature setting and the electric heating piece 6 one of heating tape 4 are provided between sample cell bracket 3 and heat-conducting seat 5
Cause, when the PCR reaction solution sample liquid levels in test tube 1 are higher by tube seat 51, remain to be heated to design temperature, the layer of heating tape 4
Number is according to liquid level difference reasonable selection.
Capping plate includes at least one top cover 21, and the bottom of top cover 21 is provided with diaphragm seal 2, by top cover 21 by diaphragm seal 2
The mouth of pipe in test tube 1 is overlayed, then clasp 10 is fastened on flange 11, realizes the sealing to test tube 1.Tube seat 51 and test tube 1
Side wall between be provided with heat conductive silica gel 52, heat conductive silica gel 52 is fixed on tube seat 51 so that heat-conducting seat 5 is more uniformly to sample
Heated.
The quantity of fluorescence detector 8 is consistent with the row of tube seat 51, the front and rear displacement in the presence of positioning screw rod 9, leads to
The diameter in hole 72 is not less than the diameter of the bottom of tube seat 51, while the rationally thickness of control radiating block 7, it is ensured that accuracy of detection, radiating
Block 7 can be the preferable copper billet of thermal diffusivity.
Claims (5)
- A kind of 1. quantitative fluorescent PCR system, it is characterised in that：Including heat-conducting seat and fluorescence detector, array on the heat-conducting seat The tube seat for holding test tubes is placed with, the top of the heat-conducting seat is provided with sample cell bracket, and the sample cell bracket is provided with With the one-to-one circular hole of tube seat, the diameter of the circular hole is less than the external diameter of the test tube mouth of pipe, and the top of the test tube is provided with capping Plate, the both sides of the capping plate are arranged with flange, and the both sides of the sample cell bracket are hinged with the clasp coordinated with flange, institute State in heat-conducting seat and be embedded with multiple electric heating pieces, the bottom of the heat-conducting seat is provided with radiating block, and the radiating block is provided with and tube seat The through hole of connection, the both sides of the radiating block are provided with heat pipe, and the lower section of the radiating block is provided with positioning screw rod, the fluoroscopic examination Device is multiple to arrange from inside to outside, and is each attached on the sliding block of positioning screw rod, and the positioning screw rod is driven by stepper motor It is dynamic.
- 2. quantitative fluorescent PCR system according to claim 1, it is characterised in that：The capping plate includes at least one top Lid, the bottom of the top cover is provided with diaphragm seal.
- 3. quantitative fluorescent PCR system according to claim 1, it is characterised in that：The sample cell bracket and heat-conducting seat it Between be provided with least one layer of heating tape.
- 4. quantitative fluorescent PCR system according to claim 1, it is characterised in that：Between the side wall of the tube seat and test tube Provided with heat conductive silica gel, the heat conductive silica gel is fixed on tube seat.
- 5. quantitative fluorescent PCR system according to claim 1, it is characterised in that：The diameter of the through hole is not less than tube seat The diameter of bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720693813.2U CN207002730U (en) | 2017-06-15 | 2017-06-15 | A kind of quantitative fluorescent PCR system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720693813.2U CN207002730U (en) | 2017-06-15 | 2017-06-15 | A kind of quantitative fluorescent PCR system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207002730U true CN207002730U (en) | 2018-02-13 |
Family
ID=61445894
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720693813.2U Active CN207002730U (en) | 2017-06-15 | 2017-06-15 | A kind of quantitative fluorescent PCR system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207002730U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110894462A (en) * | 2019-12-04 | 2020-03-20 | 苏州东胜兴业科学仪器有限公司 | PCR fluorescence detection device capable of eliminating aerosol interference |
| CN114669341A (en) * | 2020-12-24 | 2022-06-28 | 厦门致善生物科技股份有限公司 | Reaction device and medical equipment |
-
2017
- 2017-06-15 CN CN201720693813.2U patent/CN207002730U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110894462A (en) * | 2019-12-04 | 2020-03-20 | 苏州东胜兴业科学仪器有限公司 | PCR fluorescence detection device capable of eliminating aerosol interference |
| CN114669341A (en) * | 2020-12-24 | 2022-06-28 | 厦门致善生物科技股份有限公司 | Reaction device and medical equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190528 Address after: 226100 Biomedical Science and Technology Pioneer Park A9, Linjiang New District, Nantong City, Jiangsu Province Patentee after: Nantong Zhongke Gene Medical Testing Co., Ltd. Address before: 226100 Room 6, No. 208 Renmin West Road, Linjiang Town, Haimen City, Nantong City, Jiangsu Province Patentee before: Shanghai GenomePilot Technology, Inc. |
|
| TR01 | Transfer of patent right | ||
| CP03 | Change of name, title or address |
Address after: 226100 Biomedical Science and Technology Pioneer Park A9, Linjiang New District, Haimen City, Nantong City, Jiangsu Province Patentee after: Nantong Zhongke Medical Laboratory Co., Ltd Address before: 226100 Biomedical Science and Technology Pioneer Park A9, Linjiang New District, Nantong City, Jiangsu Province Patentee before: NANTONG ZHONGKE GENE MEDICAL LABORATORY Co.,Ltd. |
|
| CP03 | Change of name, title or address |