CN107034134A - A kind of rotary detection of nucleic acids cartridge - Google Patents
A kind of rotary detection of nucleic acids cartridge Download PDFInfo
- Publication number
- CN107034134A CN107034134A CN201710427242.2A CN201710427242A CN107034134A CN 107034134 A CN107034134 A CN 107034134A CN 201710427242 A CN201710427242 A CN 201710427242A CN 107034134 A CN107034134 A CN 107034134A
- Authority
- CN
- China
- Prior art keywords
- cartridge
- liquid
- detection
- spring
- reagent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 50
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 29
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 29
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 238000005213 imbibition Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 15
- 238000002347 injection Methods 0.000 claims description 9
- 239000007924 injection Substances 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 7
- 230000003321 amplification Effects 0.000 claims description 6
- 238000006073 displacement reaction Methods 0.000 claims description 5
- 239000000243 solution Substances 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 238000011002 quantification Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000001179 sorption measurement Methods 0.000 claims description 2
- 238000012546 transfer Methods 0.000 claims 2
- 230000006978 adaptation Effects 0.000 claims 1
- 238000004146 energy storage Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 230000001360 synchronised effect Effects 0.000 claims 1
- 238000000605 extraction Methods 0.000 description 7
- 244000005700 microbiome Species 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000007885 magnetic separation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
This application discloses a kind of cartridge, for Sample pretreatment and detection of nucleic acids.The cartridge includes：Pipettor, vertical guide stem, shell, closure, base, at least two sealing ring, at least two spring；Built-in accurate quantitative, constant speed, rotatable imbibition/knockout of positioning；At least one reagent chamber of base.
Description
Technical field
The invention belongs to microorganism detection field, the detection of particularly live pathogenic microorganisms, and in particular to one kind rotation
Formula detection of nucleic acids cartridge.The present invention is by paramagnetic particle method nucleic acid extraction technology and nucleic acid detection technique（Such as LAMP Turbidity measurement technologies）Phase
With reference to, closing cartridge in, realize automatic nucleic acid extraction/purifying, amplification, detection process.
Background technology
Traditional microbiological cultivation is by with specific biochemical reaction substrate, antibody, fluorescence reaction substrate, enzyme reaction
Substrate etc. is added in culture medium, and detection object is selected, separate, identified.Its operating process is cumbersome time-consuming, and has infected
Risk；Different bacterium needs different culture mediums, mostly it is non-can cultivation conditions bacterium can not be detected by culture；Different bacterium
Body produces false positive bacterium colony there may be identical specificity substance.Using magnetic separation technique and the detection of nucleic acids of LAMP technology
Method, can avoid selective enrichment culture, reduce detection time, and easily realize automation.In addition, LAMP and tradition
Nucleic acid amplification method compared to having the following advantages that：It is simple to operate, efficiently quick, high specific, high sensitivity.Certain LAMP
Have the disadvantage easily pollution, inconvenient product reclaim and identification, design space be relatively limited, amplification length 500 bp of ＜ when can not carry out
Long-chain is expanded.
Etiology is searched in epidemic-stricken area to be needed to carry out sample after long-distance transport isolation in laboratory cultures, so needs to spend
The time of several days is even more long, but for the strong virus of infectiousness, can allow the people of large area and dynamic in the very short time
Thing infects, or even dead.To save life, it is necessary to determine etiology in several hours at the scene.At the same time also to avoid
Testing result mistake caused by the cross pollution occurred in sampling, operating process.Traditional detection of nucleic acids needs to use many instrument
Device, such as instrument for extracting nucleic acid, nucleic acid augmentative instrument, fluorescence detector/gel-electrophoretic apparatus detection device.Staff's operating process is numerous
Trivial and easy infection, experimentation is open easy generation cross pollution, the shortcomings of false positive occur.
In the preceding post-production and circulation of agricultural product, the microorganism pollution of food is easily caused.Cause the detection of microorganism
It is the key link of food origin disease prevention and control, to safeguarding that consumer's is healthy significant.Food safety affair
With sudden and urgency, food pollution source testing result is also required for ageing, and the existing pathogenic bacteria detection side used
The limitation such as method or equipment have complex operation, time-consuming.
The content of the invention
It is an object of the invention to provide a kind of new rotary detection of nucleic acids cartridge, reagent needed for detection of nucleic acids is sealed up for safekeeping
In cartridge, nucleic acid extraction, amplification and the detection process of complexity are automatically performed in closing cartridge, is not only reduced cumbersome time-consuming
Hand/semi detection of nucleic acids flow and staff infection chance, and can effectively solve operating environment to reagent or
The pollution of sample and biochemical reaction Aerosol Pollution of environment etc. to external world；Built-in unique liquid relief/knockout；It is integrated positive and
Negative control group, it is ensured that the reliability of detection.
In order to realize goal of the invention, a kind of rotary detection of nucleic acids cartridge includes：Pipettor, vertical guide stem, shell, sealing
Lid, base, at least two sealing ring, at least two spring etc. are constituted, and on shell there is at least three positioning hole, at least one to be loaded
Hole, at least one shell detection hole, at least one base detection hole.
The pipettor is rotatable, inside cartridge, and chamber is replaced by injection putter, spring 1, positioning hole 2, air
Body, rotatable liquid relief arm, airway tube, liquid-transfering sucker, sealing ring etc. are constituted.
The positioning hole 2 is located above injection putter, and injection putter and spring 1 are located in air displacement cavity, and air is put
Change cavity to be tightly connected by airway tube with rotatable liquid relief arm, liquid-transfering sucker is tightly connected with rotatable liquid relief arm.Pass through outside
Power and spring 1, driving injection putter are moved back and forth, and realize accurate quantification, the imbibition of constant speed and point liquid function.
The base includes multiple reagent chamber and external screw thread feature, at least one sensing chamber in reagent chamber, and at least one heating/
Cool room, 1 sample room, at least one control group.All reagents are stored in reagent chamber with liquid or solid state in experimentation
In.Sensing chamber can use the Photoelectric Detection of the mode such as correlation, scattering formula, reflective.
The material of the base is thin, not anti-with any chemical reagent using high transparency, low reagent/sample adsorption rate, wall thickness
Should, the high molecular polymer with bio-compatibility.
There is cross positioning hole below the rotation liquid relief arm.It can realize that liquid-transfering sucker accurately rotates extremely by external impetus
Above each reagent chamber.
The positioning hole 1 and positioning hole 3 are located above vertical guide stem, and vertical guide stem is connected with rotatable liquid relief arm, spring 2
Outside rotatable liquid relief arm.By external impetus and spring 2, vertical guide stem is acted on, drives liquid-transfering sucker to be accurately positioned
To reagent chamber bottom.
The sensing chamber is with shell detection hole and base detection hole on unified hub axle.
The center of the reagent chamber is vertical with liquid-transfering sucker, is placed in liquid-transfering sucker moving range.
The base is tightly connected with shell by screw thread rotation.
The closure is connected by well with body seal.
The mode of being tightly connected has sealing ring, engagement thread etc..
The external impetus, which can be that motor, air pump, liquid pump etc. are various, provides moment of torsion, air pressure, the part of hydraulic power.And
And by microprocessor control, experiment process is automatically performed inside cartridge.
The cartridge, by the aluminium film that can use suction nozzle to pierce through, in advance seals reagent needed for detection of nucleic acids up for safekeeping.
A kind of rotary detection of nucleic acids cartridge of the present invention, reagent needed for detection of nucleic acids is sealed up for safekeeping in cartridge, in closing
Nucleic acid extraction, amplification and the detection process of complexity are automatically performed in cartridge.With advantages below.
（1）Automatically.Cartridge realizes that detection of nucleic acids is full-automatic by external impetus part, including nucleic acid extraction, amplification and
Detection.Sample is added cartridge manually, cartridge is put into instrument can fully automatic operation.
（2）Closing.Cartridge is a totally enclosed system, and the reagent chamber of base seals up for safekeeping in advance, it is ensured that sample will not be obscured,
Aerosol etc. will not be caused to pollute to environment, the infection of staff is also reduced.
（3）Quickly.Cartridge can integrate the nucleic acid extraction technology based on Magneto separate and the Turbidity measurement technology based on LAMP
Advantage, detection speed is accelerated compared to traditional detection method.
（4）It is expansible.Because cartridge base is dismountable, user, which can be actually needed, designs different cartridge bases,
It can also control to complete different experiment processes by microprocessor.
（5）Reliability.Negative and positive controls built in cartridge, it is to avoid reagent failure false negative occurs and reagent contamination is led
Cause false positive.
Brief description of the drawings
The rotary detection of nucleic acids cartridge front views of Fig. 1.
The rotary detection of nucleic acids cartridge assembling model figures of Fig. 2.
Fig. 3 cartridge base vertical views.
Wherein：Injection putter -101, spring 1-102, vertical guide stem -103, air displacement cavity -104, rotatable liquid relief
Arm -105, liquid-transfering sucker -106, spring 2-107, sealing ring -108, reagent chamber -109, screw thread -110, base -111, sealing-plug -
112nd, positioning hole 1-113, positioning hole 2-114, positioning hole 3-115, well -116, shell -117, shell detection hole -118, bottom
Seat detection hole -119.
Embodiment
The present invention relates to carry out detection of nucleic acids to microorganism in sample.Microorganism can be bacterium, virus and fungi etc..Bottom
Pre-installed in the reagent trough of seat including combining liquid, lysate, cleaning fluid, eluent, mineral oil, LAMP reaction solutions, in cartridge examination
Agent room includes a sensing chamber, and the sensing chamber uses correlation Photoelectric Detection, the detection for target dna or RNA.
The good sample of pre-treatment is filled into the sample room inside cartridge reagent chamber with Manual liquid transfering device first, then will card
Box is positioned over inside integrated detector as requested.The detector is the equipment of supporting cartridge.Under the driving of external equipment
Liquid relief component starts to handle sample liquid according to pre-set programs inside reaction box, sequentially passes through with reference to liquid, lysate, clear
The processes such as washing lotion, then move to sensing chamber by the nucleic acid solution of extraction again, mineral oil finally are added into sensing chamber.Whole detection process
Carried out under constant temperature, temperature is about provided at 60 DEG C -65 DEG C by ancillary equipment.Now the light focusing of 650nm wavelength in
Detect in indoor sample liquid, the progress detection indoor liquid reacted with LAMP can become cloudy, the photodetection to penetrating face
The photosignal that device is collected can change.Determine whether contain certain micro- life in sample by the change of photosignal
Thing.Different LAMP reaction solutions detect different microorganisms.
The preferred embodiment of the present invention is the foregoing described, still, the present invention is not limited to the tool in above-mentioned embodiment
Body details, in the range of the technology design of the present invention, can carry out a variety of equivalents, these etc. to technical scheme
Protection scope of the present invention is belonged to conversion.
Claims (9)
1. a kind of rotary detection of nucleic acids cartridge, it is characterised in that：Including at least one pipettor, vertical guide stem, shell, sealing
Lid, base, at least three positioning hole, at least one well, at least two sealing ring, at least one detection hole, at least two spring
Deng.
2. pipettor as claimed in claim 1, it is characterised in that：It is rotatable, inside cartridge, by injection putter, spring
1st, the composition such as positioning hole 2, air displacement cavity, rotatable liquid relief arm, airway tube, liquid-transfering sucker, sealing ring.
3. pipettor as claimed in claim 2, it is characterised in that：Positioning hole 2 is located above injection putter, injection putter and spring
1 is located in air displacement cavity, and air displacement cavity is tightly connected with rotatable liquid relief arm by airway tube, and liquid-transfering sucker is with that can revolve
Transfer liquid arm is tightly connected；By external impetus and spring 1, injection putter is moved back and forth, and realizes accurate quantification, the imbibition of constant speed
With a point liquid function；There is cross positioning hole below rotation liquid relief arm；It can realize that liquid-transfering sucker is pin-pointed to by external impetus
The top of each reagent chamber.
4. base as claimed in claim 1, it is characterised in that：Removably, constituted comprising multiple reagent chamber, base shirt rim has outer
At least one sensing chamber in characteristic threads, reagent chamber, at least one heating/refrigerating room, 1 sample room, at least one control group；It is real
All reagents are stored in reagent chamber with liquid or solid state during testing；According to the position of shell and base detection hole not
Together, sensing chamber can be using the Photoelectric Detection mode such as correlation, scattering formula, reflective.
5. base as claimed in claim 1, it is characterised in that：High transparency, low reagent/sample adsorption rate, wall are used using material
Thickness, not with any chemical reagent reaction, the high molecular polymer with bio-compatibility.
6. cartridge as claimed in claim 1, it is characterised in that：Positioning hole 1 and positioning hole 3 are located above vertical guide stem, vertically lead
4 guide pillar bottom faces of bar are contacted with rotatable liquid relief arm upper surface and pushed downwards by external force is synchronous, while being located to revolve
Spring 2 outside transfer liquid arm is squeezed and energy storage, after the release vertical guide stem, rotatable liquid relief arm, spring 2 all from
It is dynamic to reset；Action acts on vertical guide stem by external impetus and spring 2 above, promotes liquid-transfering sucker in institute of reagent chamber limit for height
Moved up and down in degree.
7. the external impetus as described in claim 3 and 5, it is characterised in that：Can be the various offer torsions such as motor, air pump, liquid pump
Square, air pressure, the part of hydraulic power；And by microprocessor control, experiment flow is automatically performed inside cartridge.
8. cartridge as claimed in claim 1, it is characterised in that：The chamber of the closing formed by closure, sealing ring, shell, base
Body.
9. cartridge as claimed in claim 1, it is characterised in that：Point of specific DNA/RNA fragments is expanded according to different amplifications
Sub- biology techniques principle, required reagent during detection of nucleic acids is sealed up for safekeeping in each reagent chamber in cartridge, with reference to therewith
The integrated detector of adaptation, realizes the optical signal of quantitative/qualitatively indoor reaction solution transmitting of detection detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710427242.2A CN107034134A (en) | 2017-06-08 | 2017-06-08 | A kind of rotary detection of nucleic acids cartridge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710427242.2A CN107034134A (en) | 2017-06-08 | 2017-06-08 | A kind of rotary detection of nucleic acids cartridge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107034134A true CN107034134A (en) | 2017-08-11 |
Family
ID=59541913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710427242.2A Pending CN107034134A (en) | 2017-06-08 | 2017-06-08 | A kind of rotary detection of nucleic acids cartridge |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107034134A (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653301A (en) * | 2017-10-16 | 2018-02-02 | 南京中科拜尔医学技术有限公司 | A kind of hermetic multiple fluorescent PCR detection cartridge |
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| CN107779389A (en) * | 2017-12-04 | 2018-03-09 | 宁波东夏生物科技有限公司 | Nucleic acid extraction amplification device and its application method |
| CN107841447A (en) * | 2017-12-13 | 2018-03-27 | 上海汉谟医疗科技有限公司 | A kind of apparatus and method of extraction purification nucleic acid |
| CN108315218A (en) * | 2018-05-05 | 2018-07-24 | 蔡玉华 | A kind of nucleic acid rapid extraction and detection device |
| CN108828221A (en) * | 2018-04-04 | 2018-11-16 | 美林美邦（厦门）生物科技有限公司 | A kind of sample process being provided with material transfer organization and detection reagent cup box |
| CN110006856A (en) * | 2019-05-11 | 2019-07-12 | 郑州贝贝生物科技有限公司 | A kind of Portable pesticide residue measurer |
| CN111378574A (en) * | 2020-03-25 | 2020-07-07 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN112779117A (en) * | 2019-11-07 | 2021-05-11 | 浙江大学 | Detection card box with isolation effect for nucleic acid detection experiment |
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
| CN114634853A (en) * | 2022-03-13 | 2022-06-17 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection reagent device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571740A (en) * | 2013-10-15 | 2014-02-12 | 周国华 | Device integrated with extraction, amplification and detection of nucleic acid |
| CN207210427U (en) * | 2017-06-08 | 2018-04-10 | 湖南工业大学 | A kind of rotary detection of nucleic acids cartridge |
-
2017
- 2017-06-08 CN CN201710427242.2A patent/CN107034134A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103571740A (en) * | 2013-10-15 | 2014-02-12 | 周国华 | Device integrated with extraction, amplification and detection of nucleic acid |
| CN207210427U (en) * | 2017-06-08 | 2018-04-10 | 湖南工业大学 | A kind of rotary detection of nucleic acids cartridge |
Non-Patent Citations (2)
| Title |
|---|
| ANGELIKA NIEMZ ET AL.: "Point-of-care nucleic acid testing for infectious diseases", 《TRENDS IN BIOTECHNOLOGY》 * |
| 徐任等: "采用基因分型质控品评价荧光PCR法人乳头瘤病毒核酸检测试剂盒", 《药物分析杂志》 * |
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107653301A (en) * | 2017-10-16 | 2018-02-02 | 南京中科拜尔医学技术有限公司 | A kind of hermetic multiple fluorescent PCR detection cartridge |
| CN107760602A (en) * | 2017-11-06 | 2018-03-06 | 南京中科拜尔医学技术有限公司 | A kind of cartridge for PCR primer purifying |
| CN107779389A (en) * | 2017-12-04 | 2018-03-09 | 宁波东夏生物科技有限公司 | Nucleic acid extraction amplification device and its application method |
| CN107779389B (en) * | 2017-12-04 | 2024-02-06 | 浙江拓创医疗科技有限公司 | Nucleic acid extraction amplification device and method of use thereof |
| CN107841447A (en) * | 2017-12-13 | 2018-03-27 | 上海汉谟医疗科技有限公司 | A kind of apparatus and method of extraction purification nucleic acid |
| CN110869517A (en) * | 2018-04-04 | 2020-03-06 | 美林美邦（厦门）生物科技有限公司 | Sample treatment and detection reagent cup box with material transfer structure |
| CN108828221A (en) * | 2018-04-04 | 2018-11-16 | 美林美邦（厦门）生物科技有限公司 | A kind of sample process being provided with material transfer organization and detection reagent cup box |
| WO2019192595A1 (en) * | 2018-04-04 | 2019-10-10 | 美林美邦（厦门）生物科技有限公司 | Reagent cup box for sample processing and detection provided with material transfer structure |
| CN108828221B (en) * | 2018-04-04 | 2019-11-12 | 美林美邦（厦门）生物科技有限公司 | A kind of sample process being provided with material transfer organization and detection reagent cup box |
| CN108315218A (en) * | 2018-05-05 | 2018-07-24 | 蔡玉华 | A kind of nucleic acid rapid extraction and detection device |
| CN110006856B (en) * | 2019-05-11 | 2021-08-06 | 广西达康食品配送有限公司 | Portable pesticide residue detector |
| CN110006856A (en) * | 2019-05-11 | 2019-07-12 | 郑州贝贝生物科技有限公司 | A kind of Portable pesticide residue measurer |
| CN112779117A (en) * | 2019-11-07 | 2021-05-11 | 浙江大学 | Detection card box with isolation effect for nucleic acid detection experiment |
| CN112779117B (en) * | 2019-11-07 | 2024-03-12 | 浙江大学 | Detection cartridge with isolation effect for nucleic acid detection experiment |
| CN111378574A (en) * | 2020-03-25 | 2020-07-07 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN111378574B (en) * | 2020-03-25 | 2023-10-27 | 苏州新海生物科技股份有限公司 | Nucleic acid extraction and amplification device |
| CN114058472A (en) * | 2020-07-30 | 2022-02-18 | 苏州中加康美科技有限公司 | Nucleic acid reagent reaction unit |
| CN114634853A (en) * | 2022-03-13 | 2022-06-17 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection reagent device |
| CN114634853B (en) * | 2022-03-13 | 2023-05-02 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection reagent device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107034134A (en) | A kind of rotary detection of nucleic acids cartridge | |
| CN207210427U (en) | A kind of rotary detection of nucleic acids cartridge | |
| US8647858B2 (en) | Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis | |
| CN106119101B (en) | Collect nucleic acid extraction, amplification and detects integrated device | |
| JP5616797B2 (en) | System and method for qualifying cultures as positive for microorganisms with high reliability | |
| CA2715566C (en) | Systems and methods for presumptive identification of microorganism type in a culture | |
| US10752936B2 (en) | Device for analyzing a biological sample | |
| EP2483388B1 (en) | Portable enrichment, aliquoting, and testing device of microorganisms and toxins | |
| CN106226254B (en) | A kind of micro-fluidic chip and preparation method thereof for biological detection | |
| CN113150965B (en) | Anti-pollution detection tube and CRISPR molecular diagnosis detection method based on same | |
| CN113278492B (en) | Integrated totally-enclosed detection reaction tube | |
| CN215906211U (en) | Pocket type amplification device | |
| CN104152349B (en) | A kind of detection reagent cartridge automatically | |
| CN110452806B (en) | Full-automatic micro-fluidic nucleic acid detection chip based on loop-mediated isothermal amplification, kit and method thereof | |
| Wu et al. | Rotary valve-assisted fluidic system coupling with CRISPR/Cas12a for fully integrated nucleic acid detection | |
| CN102520055A (en) | Construction method for MALDI-TOF-MS database of common pathogenic bacteria in food and animal products | |
| CN203668357U (en) | Device integrating nucleic acid extraction, amplification and detection | |
| CN111763614A (en) | Online bioaerosol monitoring system and method based on ATP (adenosine triphosphate) biochemical luminescence | |
| CN104946505B (en) | Realize PCR micro-fluidic chip and real-time PCR viral device for fast detecting | |
| US10619185B2 (en) | Automated system for the lysis of microorganisms present in a sample, for extraction and for purification of the nucleic acids of said microorganisms for purposes of analysis | |
| CN201406430Y (en) | Online coli-group fast detecting device | |
| CN207002730U (en) | A kind of quantitative fluorescent PCR system | |
| CN211035866U (en) | Plunger type nucleic acid detection integrated card box | |
| CN113913289A (en) | Detection kit and detection method for pathogenic nucleic acid under airtight condition | |
| CN107083324B (en) | Portable rapid bacterium collecting system for rapidly detecting microorganisms in filled drinking water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170811 |
|
| WD01 | Invention patent application deemed withdrawn after publication |