CN114634853B - Nucleic acid detection reagent device - Google Patents
Nucleic acid detection reagent device Download PDFInfo
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- CN114634853B CN114634853B CN202210244666.6A CN202210244666A CN114634853B CN 114634853 B CN114634853 B CN 114634853B CN 202210244666 A CN202210244666 A CN 202210244666A CN 114634853 B CN114634853 B CN 114634853B
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 72
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 72
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 72
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 49
- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 238000007789 sealing Methods 0.000 claims abstract description 200
- 229910052751 metal Inorganic materials 0.000 claims abstract description 52
- 239000002184 metal Substances 0.000 claims abstract description 52
- 239000011324 bead Substances 0.000 claims abstract description 22
- 229910052782 aluminium Inorganic materials 0.000 claims description 15
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 15
- 239000011888 foil Substances 0.000 claims description 15
- 239000000376 reactant Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 15
- 238000006243 chemical reaction Methods 0.000 abstract description 14
- 230000008569 process Effects 0.000 abstract description 11
- 230000003321 amplification Effects 0.000 abstract description 10
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 10
- 238000000605 extraction Methods 0.000 abstract description 9
- 238000012864 cross contamination Methods 0.000 abstract description 8
- 238000012546 transfer Methods 0.000 abstract description 5
- 238000010586 diagram Methods 0.000 description 7
- 238000003752 polymerase chain reaction Methods 0.000 description 7
- 230000005389 magnetism Effects 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
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- 230000004308 accommodation Effects 0.000 description 1
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present application relates to a nucleic acid detection reagent device. The nucleic acid detecting reagent device comprises: the upper seat is arranged at intervals with the kit base, the upper seat is connected with the kit base in a sealing way through a sealing structure, and a sealing cavity is formed between the upper seat and the kit base; the upper seat is provided with a metal rod, the metal rod is arranged in the sealing cavity, and the metal rod is used for being magnetically connected with a magnet outside the sealing cavity; the kit base is provided with a plurality of accommodating cavities, at least one accommodating cavity is used for accommodating magnetic beads, and a plurality of accommodating cavities can be respectively communicated with the sealing cavity. The nucleic acid detection reagent device integrates nucleic acid extraction, reagents, corresponding functional containers, amplification reaction and the like in a closed space, can effectively avoid the problem of cross contamination in the processes of nucleic acid extraction, transfer, amplification reaction and the like, and can effectively improve the detection efficiency of nucleic acid detection.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a nucleic acid detection reagent device.
Background
The magnetic bead method nucleic acid extraction kit plays an important role in the nucleic acid detection process and is one of the indispensable tools in the nucleic acid extraction and detection process. The principle is that the magnetic beads are a special nano-micron material, the size is usually between a few tenths of a micron and a few microns, the magnetic beads can move to one side rapidly in a magnetic field and gather together, the magnetic beads can quickly recover to a dispersion state after the magnetic field is removed, the surfaces of the magnetic beads for nucleic acid extraction have specific properties, the nucleic acids can be adsorbed on the surfaces under certain conditions, and the nucleic acids can be released from the surfaces under other conditions for subsequent detection steps. The traditional nucleic acid analysis has the defects of long time consumption, large equipment volume, special experiment environment and personnel requirement and the like, and due to the ultrahigh sensitivity of nucleic acid amplification, the nucleic acid amplification is carried out in the open environment of a common laboratory, and the aerosol cross contamination of a sample easily causes the false positive of the nucleic acid detection of a subsequent sample. The existing nucleic acid detection box is difficult to combine the whole nucleic acid extraction and nucleic acid detection processes into the same closed space, so that in the nucleic acid detection process, the aerosol cross contamination of a sample caused by separating the whole nucleic acid extraction and nucleic acid detection processes is easy to cause the false positive of the nucleic acid detection of a subsequent sample, and the detection efficiency is low.
Disclosure of Invention
In order to solve or partially solve the problems existing in the related art, the application provides a nucleic acid detection reagent device which has better sealing performance, can prevent cross contamination, has small volume, can be used for realizing nucleic acid analysis automation, and can effectively improve the detection efficiency of nucleic acid detection.
The present application provides a nucleic acid detecting reagent device, comprising: the upper seat is arranged at intervals with the kit base, the upper seat is connected with the kit base in a sealing way through a sealing structure, and a sealing cavity is formed between the upper seat and the kit base;
the upper seat is provided with a metal rod, the metal rod is arranged in the sealing cavity, and the metal rod is used for being magnetically connected with a magnet outside the sealing cavity;
the kit base is provided with a plurality of accommodating cavities, at least one accommodating cavity is used for accommodating magnetic beads, the plurality of accommodating cavities can be respectively communicated with the sealing cavity, and the metal rod is used for transferring the magnetic beads among the plurality of accommodating cavities.
In one embodiment, the upper seat and the metal rod are integrally formed, and the metal rod extends from the upper seat towards a direction close to one side of the kit base.
In one embodiment, the plurality of accommodating cavities are respectively provided with an opening opposite to the sealing cavity, and sealing films are arranged at the openings of the plurality of accommodating cavities and are used for packaging the plurality of accommodating cavities.
In one embodiment, the metal bar comprises a base connected to the upper seat and an end remote from the base, the base being provided with a groove; and/or
The end part is provided with a puncture part for puncturing the sealing film.
In one embodiment, the sealing structure comprises a sealing retainer ring arranged between the upper seat and the kit base, and the sealing retainer ring is respectively in sealing connection with the upper seat and the kit base.
In one embodiment, the sealing structure comprises a sealing film arranged between the upper seat and the sealing retainer ring, the upper seat and the sealing retainer ring are arranged at intervals, and the sealing film is respectively in sealing connection with the upper seat and the sealing retainer ring, and forms the sealing cavity.
In one embodiment, the sealing structure comprises a sealing ring arranged between the sealing retainer ring and the kit base, the sealing retainer ring and/or the kit base are respectively provided with a sealing groove, and the sealing ring is arranged in the sealing groove and is in contact with the sealing retainer ring and the kit base;
the sealing check ring and the kit base are respectively provided with a connecting surface which is in contact with each other and is used for forming a sealing state.
In one embodiment, the plurality of accommodating cavities are formed in the top of the kit base, the top of the kit base is the side, facing the upper base, of the kit base, at least one sample adding port communicated with the accommodating cavities is formed in the side face of the kit base, and a second sealing piece is movably arranged on the sample adding port and used for sealing the sample adding port.
In one embodiment, the nucleic acid detecting reagent device comprises a check component arranged in the sample adding port, the check component comprises a check valve arranged in the sample adding port, the check valve is propped against the port wall of the sample adding port, the check valve is provided with a liquid inlet and outlet channel, and the liquid inlet and outlet channel is used for passing through the pipette tip.
In one embodiment, the nucleic acid detection reagent device includes a pre-buried tube for containing a reactant;
the embedded pipe comprises a cylinder body and aluminum foil films arranged at two ends of the cylinder body, and the aluminum foil films are used for sealing the two ends of the cylinder body;
at least one cavity wall of the accommodating cavity is provided with a step structure, one end of the embedded pipe is connected with the step structure in a matched mode, and the step structure is sealed through the aluminum foil film.
The technical scheme provided by the application can comprise the following beneficial efficiency:
the application provides a nucleic acid detection reagent device to be formed with sealed chamber between seat and the kit base through seal structure sealing connection between seat and the kit base to the metal bar of seat is located sealed intracavity, through being connected the magnet magnetism of metal bar and sealed chamber outside, makes the metal bar have magnetism, makes the metal bar can shift between a plurality of holding the chamber on the kit base with the magnetic bead, so, has guaranteed the sealing environment of nucleic acid detection reagent device, reduces sample cross contamination's risk, can effectively promote nucleic acid detection's detection efficiency.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a schematic diagram showing the overall structure of a nucleic acid detecting reagent device according to an embodiment of the present application;
FIG. 2 is a schematic diagram showing an exploded structure of a nucleic acid detecting reagent device according to an embodiment of the present application;
FIG. 3 is a schematic diagram showing an exploded structure of a nucleic acid detecting reagent device according to another embodiment of the present application;
FIG. 4 is a schematic view showing the structure of a metal rod of a nucleic acid detecting reagent apparatus according to another embodiment of the present application;
FIG. 5 is a schematic diagram showing the structure of the upper base of a nucleic acid detecting reagent device according to an embodiment of the present application;
FIG. 6 is a schematic diagram showing the structure of a base of a kit of a nucleic acid detecting reagent device according to an embodiment of the present application;
FIG. 7 is a schematic view showing a structure of a base of a kit of a nucleic acid detecting reagent device according to another embodiment of the present application;
FIG. 8 is a schematic diagram showing the assembled upper base and kit base of a nucleic acid detecting reagent device according to an embodiment of the present disclosure;
FIG. 9 is a schematic diagram showing the structure of an embedded pipe of a nucleic acid detecting reagent device according to an embodiment of the present application.
Reference numerals:
100. an upper seat; 110. a metal rod; 111. a puncture part; 1101. a placement cavity; 1102. a groove; 120. a positioning surface; 102. a clamping hole; 130. a first seal; 103. sealing the detection hole; 104. a fixture positioning guide groove; 105. the manipulator clamps the groove; 200. a kit base; 201. a receiving chamber; 202. a sample adding port; 203. a storage hole; 210. a sealing film; 220. a second seal; 230. a step structure; 301. sealing the cavity; 401. sealing surfaces; 410. a sealing retainer ring; 402. sealing grooves; 420. a sealing film; 403. a connection surface; 430. a seal ring; 510. a guide ring; 600. pipetting gun heads; 700. a check valve; 800. a limiting ring; 900. pre-burying a pipe; 910. a cylinder; 920. an aluminum foil film.
Description of the embodiments
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the description of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate description of the present application and simplify the description, and do not indicate or imply that the system or element being referred to must have a particular orientation, be configured and operated in a particular orientation, and therefore should not be construed as limiting the present application.
Unless specifically stated or limited otherwise, the terms "mounted," "connected," "secured" and the like should be construed broadly, as they may be fixed, removable, or integral, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
In the related art, the sealing performance of the magnetic bead method nucleic acid detection reagent device is poor, so that the detection efficiency of an experimenter in using the magnetic bead method nucleic acid detection reagent device for detecting nucleic acid is poor. In order to solve or partially solve the problems existing in the related art, the application provides a nucleic acid detection reagent device, which has better sealing performance and can effectively improve the detection efficiency of nucleic acid detection.
The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.
Referring to FIGS. 1-3, the nucleic acid detecting reagent apparatus of the present embodiment includes: the upper seat 100 and the kit base 200 are arranged at intervals, the upper seat 100 and the kit base 200 are connected in a sealing way through a sealing structure, and a sealing cavity 301 is formed between the upper seat 100 and the kit base 200; the upper seat 100 is provided with a metal rod 110, the metal rod 110 is arranged in the sealing cavity 301, and the metal rod 110 is used for being magnetically connected with a magnet outside the sealing cavity 301; the kit base 200 is provided with a plurality of accommodating chambers 201, at least one accommodating chamber 201 for accommodating magnetic beads, the plurality of accommodating chambers 201 respectively being capable of communicating with the sealing chamber 301, and a metal rod for transferring magnetic beads between the plurality of accommodating chambers 201.
It can be seen from this embodiment that the upper seat 100 is in sealing connection with the kit base 200 through a sealing structure, and a sealing cavity 301 is formed between the upper seat 100 and the kit base 200, and the metal rod 110 on the upper seat 100 is disposed in the sealing cavity 301, so that the metal rod 110 has magnetism by magnetically connecting the metal rod 110 with a magnet outside the sealing cavity 301, and the metal rod 110 can transfer magnetic beads between the plurality of accommodating cavities 201 on the kit base 200, thus ensuring the sealing environment of the nucleic acid detection reagent device, reducing the risk of sample contamination, and effectively improving the detection efficiency of nucleic acid detection. The traditional nucleic acid analysis has the defects of long time consumption, large equipment volume, special experiment environment and personnel requirement and the like, and due to the ultrahigh sensitivity of nucleic acid amplification, the nucleic acid amplification is carried out in the open environment of a common laboratory, and the aerosol cross contamination of a sample easily causes the false positive of the nucleic acid detection of a subsequent sample. The nucleic acid detection reagent device can perform processes such as non-cross-contamination nucleic acid extraction, amplification and the like in a fully-closed container, can perform processes such as nucleic acid extraction, amplification reaction and the like in a fully-closed space, has better sealing performance, can prevent cross contamination, has small volume, and can be used for realizing nucleic acid analysis automation.
It should be noted that, the plurality of accommodating chambers 201 may include a lysis chamber, a cleaning chamber, a magnetic bead or magnetic bead solution storage chamber, and a PCR (polymerase chain reaction) reaction chamber, where the PCR reaction chamber may be disposed on an independent PCR tube, and then the PCR tube is hermetically connected with the kit base 200, and the PCR reaction chamber and other accommodating chambers 201 may also be formed by forming modes such as stamping forming and die casting forming on the kit base 200. In some embodiments, the thickness of the PCR reaction tube is between 0.1mm and 1.5mm, and may be specifically 0.4mm, 0.8mm, 1.0mm or 1.5mm, and the light transmittance of the reaction tube is 60% or more for optical detection.
In some embodiments, the plurality of accommodating cavities 201 are respectively provided with an opening opposite to the sealing cavity 301, and in order to ensure the sealing efficiency of the plurality of accommodating cavities 201, sealing films 210 are disposed at the openings of the plurality of accommodating cavities 201, and the sealing films 210 are used for packaging the plurality of accommodating cavities 201, it should be noted that the plurality of accommodating cavities 201 may be packaged by using a whole sealing film 210, or may be packaged by using a plurality of sealing films 210. Preferably, the sealing film 210 is an aluminum foil film.
In some embodiments, the shape of the sealing film 210 corresponds to the arrangement shape of the openings of the plurality of accommodating chambers 201, and preferably, the openings of the plurality of accommodating chambers 201 are arranged on the cartridge base 200 along a U-shaped structure, and the shape of the sealing film 210 corresponds to a U-shape.
In some embodiments, the magnitude of the magnetic properties conducted by the metal rod 110 is varied by adjusting the distance of the magnet from the metal rod 110 outside the sealed cavity 301. Illustratively, by bringing the magnet close to the metal rod 110 such that the magnetism of the metal rod 110 is enhanced, the metal rod 110 is capable of adsorbing the magnetic beads, by bringing the magnet away from the metal rod 110 such that the magnetism of the metal rod 110 is weakened, the magnetic beads adsorbed by the metal rod 110 fall down, and thus, transfer of the magnetic beads between the plurality of accommodation chambers 201 is achieved.
In some embodiments, in order to ensure the sealing efficiency of the upper seat 100 and reduce the production process of the upper seat 100, the upper seat 100 and the metal rod 110 are integrally formed, and the upper seat 100 is placed into the metal rod 110 during injection molding production, so that the metal rod 110 is embedded into the upper seat 110 and integrally formed with the upper seat 110. The metal bar 110 extends from the upper base 100 toward a side close to the cartridge base 200 so as to be close to the receiving chamber 201 on the cartridge base 200.
Referring to fig. 4, in some embodiments, the metal rod 110 includes a base portion connected to the upper seat 100 and an end portion remote from the base portion, and in order to enable the metal rod 110 to adsorb and transfer the magnetic beads in the receiving chamber 201, the end portion of the metal rod 110 is provided with a piercing portion 111 for piercing the sealing film 210 so that the receiving chamber 201 can communicate with the sealing chamber 301, so that the metal rod 110 can adsorb and transfer the magnetic beads in the receiving chamber 201. In some embodiments, the base is provided with a groove 1102, so that the base of the metal rod 110 can be firmly embedded into the upper injection molding part, and the metal rod 110 can not loosen to generate air leakage when penetrating the sealing film 210.
Referring to fig. 5 and fig. 6, in some embodiments, in order to enable the upper seat 100 to meet the requirement of industrial production, the fixture can accurately clamp the upper seat 100, and the upper seat 100 is provided with an arc-shaped positioning surface 120 for moving and positioning the fixture. In order to facilitate the fixture to stably clamp the upper seat 100, the upper seat 100 is provided with clamping holes 102, and preferably, opposite sides of the upper seat 100 are respectively provided with clamping holes 102 to improve the clamping stability of the fixture. In other embodiments, the upper seat 100 is provided with a clamp positioning guide groove 104 and a manipulator clamping groove 105, the clamp positioning guide groove 104 is disposed adjacent to the manipulator clamping groove 105, and the clamp positioning guide groove 104 has a wedge-shaped guide surface so as to guide a clamp to the manipulator clamping groove 105 along the guide surface, thereby realizing clamping of the upper seat 100. Preferably, the clamp positioning guide groove 104 and the robot clamping groove 105 have a ring-shaped structure so that the clamp can clamp the upper socket 100 from any angle.
In some embodiments, in order to ensure the sealing quality of the upper seat 100 and/or the assembly formed by assembling the upper seat 100 with other components, it is necessary to perform sealing detection on the upper seat 100 and/or the assembly formed by assembling the upper seat 100 with other components, where the upper seat 100 is provided with a sealing detection hole 103 and a first sealing member 130, where the sealing detection hole 103 is in communication with the sealing cavity 301, and the first sealing member 130 is movably connected with the sealing detection hole 103 for sealing the sealing detection hole 103. In this embodiment, the seal detection hole 103 may also be referred to as a leak detection hole, and the first seal 130 may be a sealing plug.
In some embodiments, the sealing structure comprises a sealing collar 410 disposed between the upper base 100 and the kit base 200, and the sealing collar 410 is in sealing connection with the upper base 100 and the kit base 200, respectively. In this way, by arranging the sealing check ring 410 between the upper seat 100 and the kit base 200, the sealing check ring 410 can respectively seal and connect the upper seat 100 with the kit base 200 with larger difference in shape and structure, so as to ensure the sealing quality between the upper seat 100 and the kit base 200.
In some embodiments, the sealing structure includes a sealing film 420 disposed between the upper seat 100 and the sealing collar 410, where the upper seat 100 and the sealing collar 410 are disposed at intervals, and the sealing film 420 is respectively connected with the upper seat 100 and the sealing collar 410 in a sealing manner, and forms the sealing cavity 301. In this embodiment, the sealing film 420 may be a PP (polypropylene), PE (polyethylene) or PP/PE combined composite film, and sealing may be achieved between the sealing film 420 and the upper seat 100 and between the sealing film 420 and the sealing collar 410 through a heat sealing process. In some embodiments, in order to ensure the sealing efficiency between the sealing film 420 and the upper seat 100 and between the sealing film 420 and the sealing collar 410, the upper seat 100 and the sealing collar 410 respectively have circular arc-shaped sealing surfaces 401, so that the sealing film 420 can be well paved on the sealing surfaces 401 for performing the heat sealing operation, and further ensure the sealing efficiency between the sealing film 420 and the upper seat 100 and between the sealing film 420 and the sealing collar 410. After the sealing between the sealing membrane 420 and the upper seat 100 and the sealing between the sealing membrane 420 and the sealing collar 410 is completed, a sealing chamber 301 can be formed between the sealing collar 410 and the upper seat 100.
In some embodiments, the sealing structure includes a sealing ring 430 disposed between the sealing ring 410 and the reagent kit base 200, the sealing ring 410 and/or the reagent kit base 200 are respectively provided with a sealing groove 402, and the sealing ring 430 is disposed in the sealing groove 402 and contacts the sealing ring 410 and the reagent kit base 200; the sealing ring 410 and the cartridge base 200 have connection surfaces 403 that contact each other and form a sealed state. In this embodiment, the sealing ring 410 and the kit base 200 may be welded by using a plastic welding process, and in the plastic welding process, the sealing ring 430 in the sealing groove 402 is deformed after being heated and fills the sealing groove 402, so as to realize the sealing between the sealing ring and the kit base 200. In this embodiment, the sealing groove 402 may be circumferentially disposed around the edge of the sealing ring 410 and/or the reagent cartridge base 200, and the connection surface 403 may be referred to as a welding surface, where the welding surface may be disposed on a plane opposite to the sealing ring and the reagent cartridge base 200, or may be disposed on an outer side surface of the reagent cartridge base 200, and an inner side surface of the sealing ring 410 corresponding to the outer side surface of the reagent cartridge base 200.
Referring to fig. 7, in some embodiments, the kit base 200 is provided with a storage hole 203 for storing the metal rod 110, a wall of the storage hole 203 is provided with a clamping rib, and when the metal rod 110 is pushed into the bottom of the storage hole 203, the clamping rib can clamp and fix the metal rod 110, so as to fix the whole upper base 100 above the kit base 200.
In some embodiments, the nucleic acid detecting reagent device includes a positioning structure, where the positioning structure includes a guide ring 510 provided on one of the sealing ring 410 and the reagent cartridge base 200, and a guide groove provided on the other of the sealing ring 410 and the reagent cartridge base 200, and the guide ring 510 is cooperatively connected with the guide groove. The positioning structure can ensure the assembly precision of the sealing retainer ring 410 and the kit base 200, and the guide ring 510 and the guide groove can be annular structures so as to ensure the concentricity of the connection of the sealing retainer ring 410 and the kit base 200.
In some embodiments, the kit base 200 includes a platform provided with a plurality of accommodating cavities 201, the sealing groove 402 is annularly arranged at the periphery of the platform, and the guide ring 510 is arranged at the edge of the platform and protrudes from the plane where the platform is located, so that after the guide ring 510 is matched and connected with the guide groove, the guide ring 510 can play a certain sealing role on the platform, and further the sealing efficiency of the sealing cavity 301 between the kit base 200 and the upper seat 100 is improved.
In some embodiments, the plurality of accommodating chambers 201 are formed at the top of the kit base 200, the top of the kit base 200 is a side of the kit base 200 facing the upper surface of the base 100, at least one sample adding port 202 communicated with the accommodating chambers 201 is formed at a side surface of the kit base 200, a second sealing member 220 is movably disposed at the sample adding port 202, and the second sealing member 220 is used for sealing the sample adding port 202. In this embodiment, the accommodating cavity 201 communicating with the sample inlet 202 may be a cracking cavity for generating a cracking reaction, the reaction solution may be added into the cracking cavity through the sample inlet 202, and the second sealing member 220 may be a sealing plug. To prevent loss of the second seal 220, in some embodiments, the second seal 220 is connected, preferably hinged, to the cartridge base 200.
In some embodiments, the nucleic acid testing kit is further configured for use with an external sample addition member that is movable into and out of the pipette tip 600 of the sample addition port 202. The sample adding component is a standard consumable.
In other embodiments, the nucleic acid detecting reagent device includes a check member disposed in the sample application port 202, where the check member includes a check valve 700 disposed in the sample application port 202, the check valve 700 is abutted against the wall of the sample application port 202, and the check valve 700 is provided with a liquid inlet and outlet channel for passing through the pipette tip 600. The check valve 700 may have a conical structure, and can effectively prevent the sample from flowing backward. Further, in order to further prevent the backflow of the sample injected from the sample injection port 202, the sample injection port 202 is inclined from top to bottom, so that the sample injected from the pipette tip 600 can flow into the accommodating cavity 201 along the port wall of the sample injection port 202 obliquely under the action of gravity, thereby effectively avoiding the backflow of the sample. In some embodiments, the check valve 700 is provided with the stop collar 800, the stop collar 800 is provided with the vent hole, and the center of the stop collar 800 is provided with the through hole for the pipette tip 600 to pass through, so that in the process of injecting the sample into the accommodating cavity 201, the gas in the accommodating cavity 201 can be discharged through the vent hole, and the smooth addition of the sample is ensured. The pipette tip 600 may be a consumable for sample application, and is not stored in the kit, but merely indicates a limited time state at the time of sample application at the sample application port.
Referring to fig. 8 and 9, in some embodiments, the nucleic acid detecting reagent device includes a pre-buried tube 900 for containing a reactant; the embedded pipe 900 comprises a cylinder 910 and aluminum foil films 920 arranged at two ends of the cylinder 910, wherein the aluminum foil films 920 are used for sealing two ends of the cylinder 910; the step structure 230 is formed on the cavity wall of at least one accommodating cavity 201, and one end of the pre-buried pipe 900 is connected with the step structure 230 in a matching manner, and the step structure 230 is sealed by the aluminum foil film 920. The pre-buried pipe 900 is used for pre-storing reactants, the reactants comprise liquid or solid for reaction, in the process of pre-burying the reactants, the pre-buried pipe 900 firstly uses the aluminum foil film 920 to seal one end of the cylinder 910, then the reactants are filled in the cylinder 910, and finally uses the aluminum foil film 920 to seal the other end of the cylinder 910, so that the pre-burying of the reactants is completed. The pre-buried pipe 900 is generally installed in the accommodating chamber 201 for the nucleic acid amplification reaction process, and during the reaction, aluminum foil films 920 at both ends of the pre-buried pipe 900 are pierced by the metal rod 110 so that reactants within the pre-buried pipe 900 can flow into the accommodating chamber 201 to perform the nucleic acid amplification reaction process. The embedded pipe cavity and the reagent cavity are concavely arranged at the step structure 230 of the kit base 200, the embedded pipe 900 is placed in the embedded pipe cavity, and the aluminum foil film 920 at the bottom of the embedded pipe 900 is tightly pressed and sealed with the step structure 230, so that the tightness between the embedded pipe cavity and the reagent cavity is ensured. The pre-buried pipe 900 may be used to hold reactants, reagents or other objects.
The aspects of the present application have been described in detail hereinabove with reference to the accompanying drawings. In the foregoing embodiments, the descriptions of the embodiments are focused on, and for those portions of one embodiment that are not described in detail, reference may be made to the related descriptions of other embodiments. Those skilled in the art will also appreciate that the acts and modules referred to in the specification are not necessarily required in the present application. In addition, it can be understood that the steps in the method of the embodiment of the present application may be sequentially adjusted, combined and pruned according to actual needs, and the modules in the system of the embodiment of the present application may be combined, divided and pruned according to actual needs.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (4)
1. A nucleic acid detecting reagent device, comprising:
the upper seat is arranged at intervals with the kit base, the upper seat is connected with the kit base in a sealing way through a sealing structure, and a sealing cavity is formed between the upper seat and the kit base;
the upper seat is provided with a metal rod, the metal rod is arranged in the sealing cavity, and the metal rod is used for being magnetically connected with a magnet outside the sealing cavity;
the kit base is provided with a plurality of accommodating cavities, at least one accommodating cavity is used for accommodating magnetic beads, and the plurality of accommodating cavities can be respectively communicated with the sealing cavity;
the sealing structure comprises a sealing check ring arranged between the upper seat and the kit base, and the sealing check ring is respectively in sealing connection with the upper seat and the kit base;
the sealing structure comprises a sealing film arranged between the upper seat and the sealing check ring, the upper seat and the sealing check ring are arranged at intervals, and the sealing film is respectively in sealing connection with the upper seat and the sealing check ring, and forms the sealing cavity;
the sealing structure comprises a sealing ring arranged between the sealing check ring and the kit base, wherein the sealing ring and/or the kit base are/is respectively provided with a sealing groove, and the sealing ring is arranged in the sealing groove and is contacted with the sealing check ring and the kit base;
the sealing check ring and the kit base are respectively provided with a connecting surface which is in contact with each other and is used for forming a sealing state;
the plurality of accommodating cavities are respectively provided with an opening opposite to the sealing cavity, sealing films are arranged at the openings of the plurality of accommodating cavities, and the sealing films are used for packaging the plurality of accommodating cavities;
the metal rod comprises a base and an end, the base is connected with the upper seat, the end is far away from the base, and the base is provided with a groove; and/or
The end part is provided with a puncture part for puncturing the sealing film;
the nucleic acid detection reagent device comprises a pre-buried pipe for accommodating reactants;
the embedded pipe comprises a cylinder body and aluminum foil films arranged at two ends of the cylinder body, and the aluminum foil films are used for sealing the two ends of the cylinder body;
at least one cavity wall of the accommodating cavity is provided with a step structure, one end of the embedded pipe is connected with the step structure in a matched mode, and the step structure is sealed through the aluminum foil film.
2. The nucleic acid detecting reagent device according to claim 1, wherein:
the upper seat and the metal rod are of an integrated structure, and the metal rod extends from the upper seat towards the direction close to one side of the kit base.
3. The nucleic acid detecting reagent device according to claim 1, wherein:
the kit comprises a kit base, a plurality of accommodating cavities, a second sealing piece and a sealing plate, wherein the accommodating cavities are formed in the top of the kit base, the top of the kit base faces one side of the upper base, at least one sample adding port communicated with the accommodating cavities is formed in the side face of the kit base, and the second sealing piece is movably arranged on the sample adding port and is used for sealing the sample adding port.
4. The nucleic acid detecting reagent device according to claim 3, wherein:
the nucleic acid detection reagent device comprises a check component arranged in the sample adding port, the check component comprises a check valve arranged in the sample adding port, the check valve is propped against the port wall of the sample adding port, the check valve is provided with a liquid inlet and outlet channel, and the liquid inlet and outlet channel is used for allowing a pipette tip to pass through.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210244666.6A CN114634853B (en) | 2022-03-13 | 2022-03-13 | Nucleic acid detection reagent device |
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| CN202210244666.6A CN114634853B (en) | 2022-03-13 | 2022-03-13 | Nucleic acid detection reagent device |
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| CN114634853B true CN114634853B (en) | 2023-05-02 |
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| CN114836308B (en) * | 2022-03-13 | 2023-03-21 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection kit and preparation process thereof |
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Effective date of registration: 20240201 Address after: 510000 room 603, building G12, No. 31 Kefeng Road, Huangpu District, Guangzhou, Guangdong Patentee after: Guangzhou Diao Gene Technology Co.,Ltd. Country or region after: China Address before: 510663 room 604, building G12, No. 31, Kefeng Road, Huangpu District, Guangzhou City, Guangdong Province Patentee before: SHOUXI (GUANGZHOU) MEDICAL TECHNOLOGY Co.,Ltd. Country or region before: China |