CN112852626A - Integrated sample detection device and detection method thereof - Google Patents
Integrated sample detection device and detection method thereof Download PDFInfo
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- CN112852626A CN112852626A CN202110301008.1A CN202110301008A CN112852626A CN 112852626 A CN112852626 A CN 112852626A CN 202110301008 A CN202110301008 A CN 202110301008A CN 112852626 A CN112852626 A CN 112852626A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention provides an integrated sample detection device and a detection method thereof, wherein the device comprises a magnetic sleeve and a detection box; the detection box is provided with at least four cavities which are arranged side by side and are not communicated with each other, the top of the detection box is provided with a sealing structure for sealing all the cavities, the magnetic sleeve can pierce the sealing structure, and the magnetic sleeve can extend into each cavity; the cavity comprises a lysis cavity for lysing a sample, a magnetic bead cavity pre-filled with magnetic beads, a washing cavity for washing and an elution cavity for elution; a reaction tube is fixed at the bottom of the elution cavity, the top of the reaction tube is sealed, and a detection reactant is pre-filled in the reaction tube; the other chambers except the magnetic bead chamber are respectively pre-filled with different reagents for sample extraction. The detection box has the advantages of simple structure, low cost, one-time use, no cross contamination among samples and high safety, and provides wide prospect for further applying the fluorescent quantitative PCR technology to clinic.
Description
Technical Field
The invention relates to the technical field of detection, in particular to an integrated sample detection device and a detection method thereof.
Background
The main application of molecular detection in medical inspection is the detection of the infectivity of pathogenic microorganisms. The mainstream technology of molecular detection is fluorescence quantitative PCR technology, and most of the traditional products are open consumables due to the characteristics of exponential amplification template of PCR technology, for example, chinese patents with publication numbers CN106442454A and publication number 2017.2.22: the detection kit used in the method is an open consumable material, and factors such as aerosol pollution, multiple experimental steps, complex operation, low automation degree, unstable result and the like caused by the open consumable material become important conditions for limiting the further application of the fluorescent quantitative PCR technology to clinic.
Conventional experiment operations are mostly performed in an open experimental environment, and samples can directly contact with the outside through air, but some samples which are harmful to operators and easy to be interfered by the outside or interfere with the outside, such as biological samples containing fulminating infectious diseases, are often present, so that in order to reduce biological hazards and reduce manual operations, a closed device for sample pretreatment and target substance detection is urgently needed to be invented.
Disclosure of Invention
The invention provides an integrated sample detection device and a detection method thereof, wherein the detection device is simple in structure, low in cost, disposable, free of cross contamination among samples and high in safety, and provides a wide prospect for further applying a fluorescent quantitative PCR technology to clinic.
The technical scheme of the invention is as follows:
an integrated sample detection device comprises a magnetic sleeve and a detection box;
the detection box is provided with at least four cavities which are arranged side by side and are not communicated with each other, a sealing structure is arranged at the top of the detection box to seal all the cavities, the magnetic sleeve can pierce the sealing structure, and the magnetic sleeve can extend into each cavity;
the cavity comprises a lysis cavity for lysing a sample, a magnetic bead cavity pre-filled with magnetic beads, a washing cavity for washing and an elution cavity for elution;
a reaction tube is fixed at the bottom of the elution cavity, the top of the reaction tube is sealed, and a detection reactant is pre-filled in the reaction tube;
the other cavities except the magnetic bead cavity are respectively pre-filled with different reagents for sample extraction.
The detection box comprises a plurality of cavities, and different cavities can be preloaded with different sample extraction reagents; the sealing structure is tightly contacted with the top of the detection box to play a role of sealing the cavity, and the reagent in the cavity is limited in the cavity; can insert the magnetism sleeve into every cavity through the instrument, the magnetic rod on the instrument can stretch into magnetism sleeve the inside to adsorb the magnetic bead in the reagent, through removing the magnetic bead to different cavities, thereby realize shifting the material in the cavity, and then finally shift the target thing to and react in the reaction tube and detect.
Furthermore, two washing cavities are arranged, including the first washing cavity and the second washing cavity, so that the sample can be washed more cleanly.
Furthermore, the magnetic sleeve is in a hollow cylindrical tube shape, the bottom end of the magnetic sleeve is closed to form a cone shape, and the top end of the magnetic sleeve is opened.
Furthermore, the reaction tube is a conical sleeve matched with the bottom end of the magnetic sleeve.
The magnetic rod can be inserted into the magnetic sleeve through the opening, so that the magnetic sleeve is provided with magnetism to adsorb magnetic beads in the reagent. Meanwhile, when the conical shape of the bottom end of the magnetic sleeve is inserted into the reaction tube, the reaction tube can be blocked and sealed, and the air tightness is further enhanced.
Further, the cross section of the cavity is one of a triangle, a pitch, a polygon, an ellipse and a circle.
Further, the sealing structure is a sealing film or a sealing cover.
Further, the sealing film is made of a plastic film or an aluminum film and can be quickly pierced, and the sealing film is sealed by heat sealing or ultrasonic welding.
Further, one side of the cartridge extends outward to form a tab, and one end of the sealing film extends to the tab to be covered. Can tear the one corner to the seal membrane along on the lug, add the detection sample in splitting the cavity, cover the seal membrane again, in the time of convenient operation, reinforcing leakproofness.
Furthermore, the reaction tube is a PCR tube, and the bottom of the reaction tube is externally connected to a detection instrument.
The invention also provides a method for detecting by using the integrated sample detection device, which comprises the following steps:
(a) sample adding: opening a sealing film on the cracking cavity, adding a detection sample, covering the sealing film, and then putting the integrated sample detection box into a detection instrument according to a positioning structure;
(b) sample extraction and PCR detection:
1) puncturing a sealing film: the detection instrument moves the magnetic sleeve to the position above one of the cavities, then pierces the sealing films at the corresponding positions by the magnetic sleeve, and repeats the steps to pierce the sealing films on all the cavities;
2) sample lysis: the detection instrument moves the magnetic sleeve into the cracking cavity, and then the magnetic sleeve moves up and down or rotates in the cavity to uniformly mix and crack the detection sample in the cavity;
3) target capture: the detection instrument moves the magnetic sleeve into the magnetic bead cavity, so that the magnetic sleeve moves up and down or rotates in the cavity to uniformly mix the magnetic beads in the cavity; a magnetic bar on the detection instrument is downwards inserted into the magnetic sleeve to adsorb the magnetic beads in the cavity and adsorb the magnetic beads onto the magnetic sleeve; the detection instrument moves the magnetic sleeve and the magnetic rod together into the cracking cavity, withdraws the magnetic rod, releases the magnetic beads adsorbed on the magnetic sleeve, and the magnetic sleeve moves up and down in the cavity to uniformly mix the reagent and the magnetic beads in the cavity, so that the target in the detection sample is adsorbed on the magnetic beads;
4) washing: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve, and the magnetic sleeve adsorbs magnetic beads in the cracking cavity; the magnetic sleeve and the magnetic rod move together into the washing cavity, the magnetic rod is removed, the magnetic beads adsorbed on the magnetic sleeve are released, and the magnetic sleeve moves up and down in the washing cavity to uniformly mix the reagent and the magnetic beads in the cavity; then transferring the magnetic beads to another washing cavity by the same method for secondary washing;
5) eluting a target substance: the detection instrument moves the magnetic sleeve and the magnetic rod together into the elution cavity, the magnetic rod is withdrawn, the magnetic beads adsorbed on the magnetic sleeve are released, and the magnetic sleeve moves up and down in the elution cavity to uniformly mix the reagent and the magnetic beads in the cavity;
6) discarding magnetic beads: a magnetic rod of the detection instrument is downwards inserted into the magnetic sleeve to adsorb the magnetic beads in the elution cavity, and the magnetic beads are adsorbed onto the magnetic sleeve; the detection instrument moves the magnetic sleeve and the magnetic rod together into the washing cavity, withdraws the magnetic rod, discards the magnetic beads adsorbed on the magnetic sleeve in the washing cavity, finishes the extraction of the target, and mixes the cleaned target in the reagent in the elution cavity;
7) and (3) target object detection: the detection instrument moves the magnetic sleeve into the elution cavity, then moves the magnetic sleeve downwards all the time, the bottom end of the magnetic sleeve pierces the top of the reaction tube, so that the reagent containing the target object in the elution cavity flows into the reaction tube below, the magnetic sleeve seals the tube opening of the reaction tube at the moment, the reagent containing the target object is mixed with the detection reagent in the reaction tube, and the detection instrument starts a PCR program for detection;
(c) the device discards: and after the PCR detection is finished, taking the integrated sample detection box out of the detector for performing waste biological treatment.
The invention has the beneficial effects that:
the invention integrates the sample pretreatment and the separation, extraction and detection of the target object into a detection box, so that the preparation and the detection of nucleic acid or protein and the like can be realized in a closed space, manual sample pretreatment is not needed, and the seamless butt joint and the function integration in the whole process are realized. The detection box greatly simplifies the whole structure, is disposable, has no cross contamination among samples, reduces the probability of sample contamination, improves the accuracy of sample extraction and detection, can be added with liquid samples and swab samples, only needs one step of sample addition operation, is convenient to use, and provides wide prospect for further applying the fluorescent quantitative PCR technology to clinic.
Drawings
FIG. 1 is a schematic view showing the construction of a cartridge according to the present invention;
in the figure: detection box 1, first cavity 101, second cavity 102, third cavity 103, fourth cavity 104, fifth cavity 105, sixth cavity 106, lug 107, sealing film 2, magnetic bead 3, magnetic sleeve 4 and reaction tube 5.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
Example 1:
as shown in fig. 1, an integrated sample detection device comprises a magnetic sleeve 4 and a detection box 1, wherein the detection box 1 is made of transparent materials, the detection box 1 is provided with six cavities which are parallel and equal in height and are not communicated with each other, the cavities are all in a strip-shaped structure, and a sealing film 2 is arranged at the top of each cavity for sealing all the cavities; the second cavity 102 is a magnetic bead cavity pre-filled with magnetic beads 3, the magnetic sleeve 4 is placed in the third cavity 103, the first cavity 101 is a cracking cavity for cracking a sample, the fourth cavity 104 is a first washing cavity for washing, the fifth cavity 105 is a second washing cavity for washing, the sixth cavity 106 is an elution cavity for elution, and different reagents for sample extraction are pre-filled in the first, fourth, fifth and sixth cavities respectively; wherein, the bottom of the cavity VI 106 is fixed with a reaction tube 5, a detection reagent is pre-loaded in the reaction tube 5, the top of the reaction tube 5 is sealed, the reaction tube 5 is a PCR tube, and the bottom of the reaction tube 5 is externally connected with a detection instrument.
In this embodiment, the magnetic sleeve 4 is a hollow cylindrical tube, the bottom end of the magnetic sleeve 4 is closed, the top end of the magnetic sleeve is open, the bottom end of the magnetic sleeve 4 is tapered, and the reaction tube 5 is a tapered sleeve matched with the bottom end of the magnetic sleeve 4. The magnetic rod can be inserted into the magnetic sleeve 4 through the opening, so that the magnetic sleeve 4 can adsorb the magnetic beads 3 in the reagent with magnetism. Meanwhile, when the conical shape of the bottom end of the magnetic sleeve 4 is inserted into the reaction tube 5, the reaction tube 5 can be blocked and sealed, and the air tightness is further enhanced.
In this embodiment, the cross section of the cavity is one of a triangle, a pitch, a polygon, an ellipse, and a circle, and may be other irregular shapes, which can be formulated as required.
In this embodiment, the sealing film 2 is made of a plastic film, an aluminum film, or the like, and can be pierced quickly, and the sealing film 2 is sealed by heat sealing or ultrasonic welding.
In this embodiment, one side of the first chamber 101 extends outward to form a tab 107, and one end of the sealing film 2 extends to the tab 107 to cover. The sealing film 2 can be torn off along the lug 107, a detection sample can be added into the first cavity 101, and the sealing film 2 is covered again, so that the sealing performance is enhanced while the operation is convenient.
The magnetic sleeve 4 can be moved back and forth and up and down to a required position by an external detection instrument; the detection instrument takes out the magnetic sleeve 4 after piercing the sealing film 2, moves back and forth to enable the magnetic sleeve 4 to be positioned above the next cavity, then moves the magnetic sleeve 4 downwards into the cavity, the magnetic sleeve 4 moves up and down or rotates in the cavity to uniformly mix reagents in the cavity, and a magnetic rod on the detection instrument is downwards inserted into the magnetic sleeve 4 to adsorb the magnetic beads 3 in the cavity; taking out the magnetic sleeve 4 together with the magnetic rod, moving the magnetic sleeve 4 back and forth to enable the magnetic sleeve 4 to be positioned above the next cavity, then moving the magnetic sleeve 4 together with the magnetic rod downwards into the cavity, withdrawing the magnetic rod, releasing the magnetic beads 3 adsorbed on the magnetic sleeve 4, and moving the magnetic sleeve 4 up and down in the cavity to uniformly mix the reagent in the cavity; repeating the steps, and moving the magnetic beads 3 to different cavities to transfer the materials in the cavities, so that the target is finally transferred to the reaction tube 5 for reaction detection.
The invention integrates the sample pretreatment and the separation, extraction and detection of the target object into a detection box, so that the preparation and the detection of nucleic acid or protein and the like can be realized in a closed space, manual sample pretreatment is not needed, and the seamless butt joint and the function integration in the whole process are realized. The whole structure is greatly simplified, the device is disposable, cross contamination among samples is avoided, the probability of sample contamination is reduced, and the accuracy of sample extraction and detection is improved.
The invention is further described below by taking nucleic acid extraction and PCR detection as examples, wherein a lysis solution is pre-filled in the first cavity 101, a washing solution with a lower concentration is pre-filled in the fourth cavity 104, a washing solution with a higher concentration is pre-filled in the fifth cavity 105, and an eluent is pre-filled in the sixth cavity 106, and the specific detection process comprises the following steps:
(a) sample adding: opening the sealing film 2 on the first cavity 101, adding a detection sample, covering the sealing film 2, and then putting the integrated sample detection box into a detection instrument according to a positioning structure;
(b) sample extraction and PCR detection:
1) puncturing the sealing film 2: the detection instrument pierces the sealing film 2 on the third cavity 103 and extends into the third cavity 103 to take out the magnetic sleeve 4, then the magnetic sleeve 4 is moved to the upper part of the other cavity, the sealing film 2 at the corresponding position is pierced by the magnetic sleeve 4, the steps are repeated, and the sealing films 2 on all the cavities are pierced;
2) sample lysis: the detection instrument moves the magnetic sleeve 4 into the first cavity 101, and then the magnetic sleeve 4 moves up and down or rotates in the cavity to uniformly mix and crack the detection sample in the cavity;
3) and (3) nucleic acid capture: the detection instrument moves the magnetic sleeve 4 into the second cavity 102, so that the magnetic sleeve 4 moves up and down or rotates in the cavity to uniformly mix the magnetic beads 3 in the cavity; a magnetic rod on the detection instrument is downwards inserted into the magnetic sleeve 4 to adsorb the magnetic beads 3 in the cavity, and the magnetic beads 3 are adsorbed on the magnetic sleeve 4; the detection instrument moves the magnetic sleeve 4 and the magnetic rod together into a first cavity, withdraws the magnetic rod, releases the magnetic beads 3 adsorbed on the magnetic sleeve 4, and the magnetic sleeve 4 moves up and down in the cavity to uniformly mix the lysate and the magnetic beads 3 in the cavity, so that the target in the detection sample is adsorbed on the magnetic beads 3;
4) washing: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve 4, and the magnetic sleeve 4 adsorbs the magnetic beads 3 in the first cavity 101; the magnetic sleeve 4 and the magnetic rod move together into the fourth cavity 104, the magnetic rod is withdrawn, the magnetic beads 3 adsorbed on the magnetic sleeve 4 are released, and the magnetic sleeve 4 moves up and down in the fourth cavity 104 to uniformly mix the washing liquid and the magnetic beads 3 in the fourth cavity 104; then, the magnetic beads 3 are transferred into the cavity 105 with the same method for secondary cleaning;
5) nucleic acid elution: the detection instrument moves the magnetic sleeve 4 and the magnetic rod together into the No. six cavity 106, withdraws the magnetic rod, releases the magnetic beads 3 adsorbed on the magnetic sleeve 4, and the magnetic sleeve 4 moves up and down in the No. six cavity 106 to uniformly mix the eluent and the magnetic beads 3 in the cavity;
6) discarding magnetic beads: a magnetic rod of the detection instrument is downwards inserted into the magnetic sleeve 4 to adsorb the magnetic beads 3 in the cavity No. six 106, and the magnetic beads 3 are adsorbed onto the magnetic sleeve 4; the detection instrument moves the magnetic sleeve 4 and the magnetic rod together into the fifth cavity 105, withdraws the magnetic rod, discards the magnetic beads 3 adsorbed on the magnetic sleeve 4 in the fifth cavity 105, completes nucleic acid extraction, and mixes the cleaned nucleic acid in the eluent of the sixth cavity 106;
7) and (3) nucleic acid detection: the detection instrument moves the magnetic sleeve 4 into the number six cavity 106, then moves the magnetic sleeve 4 downwards all the time, the bottom end of the magnetic sleeve 4 pierces the top of the reaction tube 5, the eluent containing nucleic acid in the number six cavity 106 flows into the reaction tube 5 below, the magnetic sleeve 4 plugs and seals the tube opening of the reaction tube 5 at the moment, the eluent containing nucleic acid is mixed with the detection reactant in the reaction tube 5, and the detection instrument starts a PCR program to perform nucleic acid detection;
(c) the device discards: and after the PCR detection is finished, taking the integrated sample detection box out of the detector for performing waste biological treatment.
The integrated sample detection device provided by the invention reduces the possibility of sample pollution by adopting disposable consumables, has a simple structure and can reduce consumable cost. The invention can be used for adding liquid samples and swab samples, only needs one-step sample adding operation, is convenient to use, and provides wide prospect for further applying the fluorescent quantitative PCR technology to clinic.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (10)
1. An integrated sample detection device is characterized by comprising a magnetic sleeve and a detection box;
the detection box is provided with at least four cavities which are arranged side by side and are not communicated with each other, a sealing structure is arranged at the top of the detection box to seal all the cavities, the magnetic sleeve can pierce the sealing structure, and the magnetic sleeve can extend into each cavity;
the cavity comprises a lysis cavity for lysing a sample, a magnetic bead cavity pre-filled with magnetic beads, a washing cavity for washing and an elution cavity for elution;
a reaction tube is fixed at the bottom of the elution cavity, the top of the reaction tube is sealed, and a detection reactant is pre-filled in the reaction tube;
the other cavities except the magnetic bead cavity are respectively pre-filled with different reagents for sample extraction.
2. The integrated sample testing device of claim 1, wherein there are two wash chambers, including a first wash chamber and a second wash chamber.
3. The integrated sample testing device according to claim 1, wherein said magnetic sleeve is a hollow cylindrical tube with a closed conical bottom end and an open top end.
4. The integrated sample testing device according to claim 3, wherein said reaction tube is a tapered sleeve fitted to a bottom end of said magnetic sleeve.
5. The integrated sample testing device of claim 1, wherein the cross-section of the cavity is one of triangular, rectangular, polygonal, elliptical, and circular.
6. The integrated sample testing device according to claim 1, wherein said sealing structure is a sealing film or a sealing cap.
7. The integrated sample testing device according to claim 6, wherein the sealing film is a plastic film or an aluminum film, and the sealing film is sealed by heat sealing or ultrasonic welding.
8. The integrated sample testing device of claim 1, wherein one side of the cartridge extends outward to form a tab, and one end of the sealing membrane extends over the tab for covering.
9. The integrated sample detection device according to claim 1, wherein the reaction tube is a PCR tube, and the bottom of the reaction tube is externally connected to the detection instrument.
10. A method of performing an assay using the unitary sample testing device of any of claims 1-9, comprising the steps of:
(a) sample adding: opening a sealing film on the cracking cavity, adding a detection sample, covering the sealing film, and then putting the integrated sample detection box into a detection instrument according to a positioning structure;
(b) sample extraction and PCR detection:
1) puncturing a sealing film: the detection instrument moves the magnetic sleeve to the position above one of the cavities, then pierces the sealing films at the corresponding positions by the magnetic sleeve, and repeats the steps to pierce the sealing films on all the cavities;
2) sample lysis: the detection instrument moves the magnetic sleeve into the cracking cavity, and then the magnetic sleeve moves up and down or rotates in the cavity to uniformly mix and crack the detection sample in the cavity;
3) target capture: the detection instrument moves the magnetic sleeve into the magnetic bead cavity, so that the magnetic sleeve moves up and down or rotates in the cavity to uniformly mix the magnetic beads in the cavity; a magnetic bar on the detection instrument is downwards inserted into the magnetic sleeve to adsorb the magnetic beads in the cavity and adsorb the magnetic beads onto the magnetic sleeve; the detection instrument moves the magnetic sleeve and the magnetic rod together into the cracking cavity, withdraws the magnetic rod, releases the magnetic beads adsorbed on the magnetic sleeve, and the magnetic sleeve moves up and down in the cavity to uniformly mix the reagent and the magnetic beads in the cavity, so that the target in the detection sample is adsorbed on the magnetic beads;
4) washing: a magnetic rod of the detection instrument is downwards inserted into a magnetic sleeve, and the magnetic sleeve adsorbs magnetic beads in the cracking cavity; the magnetic sleeve and the magnetic rod move together into the washing cavity, the magnetic rod is removed, the magnetic beads adsorbed on the magnetic sleeve are released, and the magnetic sleeve moves up and down in the washing cavity to uniformly mix the reagent and the magnetic beads in the cavity; then transferring the magnetic beads to another washing cavity by the same method for secondary washing;
5) eluting a target substance: the detection instrument moves the magnetic sleeve and the magnetic rod together into the elution cavity, the magnetic rod is withdrawn, the magnetic beads adsorbed on the magnetic sleeve are released, and the magnetic sleeve moves up and down in the elution cavity to uniformly mix the reagent and the magnetic beads in the cavity;
6) discarding magnetic beads: a magnetic rod of the detection instrument is downwards inserted into the magnetic sleeve to adsorb the magnetic beads in the elution cavity, and the magnetic beads are adsorbed onto the magnetic sleeve; the detection instrument moves the magnetic sleeve and the magnetic rod together into the washing cavity, withdraws the magnetic rod, discards the magnetic beads adsorbed on the magnetic sleeve in the washing cavity, finishes the extraction of the target, and mixes the cleaned target in the reagent in the elution cavity;
7) and (3) target object detection: the detection instrument moves the magnetic sleeve into the elution cavity, then moves the magnetic sleeve downwards all the time, the bottom end of the magnetic sleeve pierces the top of the reaction tube, so that the reagent containing the target object in the elution cavity flows into the reaction tube below, the magnetic sleeve seals the tube opening of the reaction tube at the moment, the reagent containing the target object is mixed with the detection reagent in the reaction tube, and the detection instrument starts a PCR program for detection;
(c) the device discards: and after the PCR detection is finished, taking the integrated sample detection box out of the detector for performing waste biological treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110301008.1A CN112852626A (en) | 2021-03-22 | 2021-03-22 | Integrated sample detection device and detection method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110301008.1A CN112852626A (en) | 2021-03-22 | 2021-03-22 | Integrated sample detection device and detection method thereof |
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| CN112852626A true CN112852626A (en) | 2021-05-28 |
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| CN202110301008.1A Pending CN112852626A (en) | 2021-03-22 | 2021-03-22 | Integrated sample detection device and detection method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107035A (en) * | 2021-11-30 | 2022-03-01 | 珠海黑马生物科技有限公司 | Closed full-automatic nucleic acid extraction kit |
| CN114634853A (en) * | 2022-03-13 | 2022-06-17 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection reagent device |
| WO2023197459A1 (en) * | 2022-04-15 | 2023-10-19 | 广东润鹏生物技术有限公司 | Reagent cartridge, detection device, and detection method |
-
2021
- 2021-03-22 CN CN202110301008.1A patent/CN112852626A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107035A (en) * | 2021-11-30 | 2022-03-01 | 珠海黑马生物科技有限公司 | Closed full-automatic nucleic acid extraction kit |
| CN114634853A (en) * | 2022-03-13 | 2022-06-17 | 首玺(广州)医疗科技有限责任公司 | Nucleic acid detection reagent device |
| WO2023197459A1 (en) * | 2022-04-15 | 2023-10-19 | 广东润鹏生物技术有限公司 | Reagent cartridge, detection device, and detection method |
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