CN217359144U - Nucleic acid release sampling device - Google Patents
Nucleic acid release sampling device Download PDFInfo
- Publication number
- CN217359144U CN217359144U CN202121729338.2U CN202121729338U CN217359144U CN 217359144 U CN217359144 U CN 217359144U CN 202121729338 U CN202121729338 U CN 202121729338U CN 217359144 U CN217359144 U CN 217359144U
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- Prior art keywords
- nucleic acid
- test tube
- sampling device
- acid release
- sample
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- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 42
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 42
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 42
- 238000005070 sampling Methods 0.000 title claims abstract description 36
- 238000012360 testing method Methods 0.000 claims abstract description 33
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 5
- 238000003745 diagnosis Methods 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 3
- 238000009434 installation Methods 0.000 abstract description 2
- 238000011017 operating method Methods 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 18
- 239000011324 bead Substances 0.000 description 7
- 238000000605 extraction Methods 0.000 description 6
- 239000004743 Polypropylene Substances 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 150000003839 salts Chemical class 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- -1 Polypropylene Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000003196 chaotropic effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 229920001155 polypropylene Polymers 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 238000005185 salting out Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- YTRQFSDWAXHJCC-UHFFFAOYSA-N chloroform;phenol Chemical compound ClC(Cl)Cl.OC1=CC=CC=C1 YTRQFSDWAXHJCC-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a nucleic acid release sampling device, it can include test tube, inner cup and sample burette, the test tube holds nucleic acid release agent, inner cup detachably seal installation be in on the mouth of pipe of test tube, the sample burette is sealed wears to locate the inner cup to its body stretches into in the test tube and the rubber head dew is outside the inner cup. The utility model discloses left out the operating procedure with the pipettor absorption sample, reduced the pollution risk that brings in the operation, simplified the use of instrument and equipment, improved the detection efficiency of timely diagnosis, reduced human cost and protective articles cost.
Description
Technical Field
The utility model relates to the field of medical equipment, specifically relate to a nucleic acid release sampling device.
Background
Common methods for extracting nucleic acid include an extraction method based on phenol chloroform for denaturing protein, a salting-out method for precipitating protein with high salt, a glass bead method for adsorbing nucleic acid with glass beads, a silica gel column method for adsorbing nucleic acid with high salt and dissociating nucleic acid with low salt, and a magnetic bead method for adsorbing nucleic acid with functional groups. The phenol chloroform extraction method has the greatest advantages of economy, flexibility, high purity of extracted nucleic acid, complex extraction process, influence on the integrity of the nucleic acid due to improper operation and use of toxic organic solvents. The salting-out method has the greatest advantages of economy, material benefit and safety, but the impurities are not completely removed. The principle of the glass bead method is that the glass beads are mainly used for carrying out adsorption reaction with nucleic acid under the condition of high chaotropic salt (guanidine hydrochloride, NaI and guanidine isothiocyanate), and the nucleic acid is eluted under the condition of low chaotropic salt concentration, so that the separation of the nucleic acid from impurities such as protein, sugar, lipid and the like is achieved, but the method is not adopted by most companies at present due to the residue of the glass beads and the influence of the drying problem. The silica gel column method (filter membrane method) changes extraction into filtration operation by using a filter membrane, greatly reduces the centrifugation and drying time of the conventional method, and omits the process of precipitating nucleic acid by alcohols, but the operation process is too complicated and the extraction time is long. The magnetic bead method has the greatest advantages of automation, good repeatability, complete elimination of manual processes such as centrifugation and the like of the traditional method, and long extraction process time.
At present, a common nucleic acid releasing agent reaction tube on the market is a single tube and contains a sample releasing agent with a certain volume, when the reaction tube is used, a mode that the sample and the releasing agent are mixed in equal proportion is adopted, or the whole swab is put into the tube for extraction, but finally, the extracted sample needs to be taken out and loaded by a liquid transfer device for experiment, so that the operation is more complicated, and the pollution risk in the sampling process is increased.
Disclosure of Invention
The utility model aims at providing a nucleic acid release sampling device to solve above-mentioned problem. Therefore, the utility model discloses a specific technical scheme as follows:
a nucleic acid release sampling device can include test tube, inner cup and sample burette, the test tube holds the nucleic acid release agent, inner cup detachably seal installation is in on the mouth of pipe of test tube, the seal of sample burette wears to locate the inner cup to its body stretches into in the test tube and the rubber head is exposed outside the inner cup.
Further, the spout has an internal thread, and the inner cap has an external thread.
Furthermore, the body of the sampling dropper is a glass tube, and scales are arranged on the glass tube.
Further, the diameter of the tube of the sampling dropper is gradually reduced.
Further, the end of the sampling dropper is less than 0.5cm from the bottom of the test tube.
Further, the nucleic acid release sampling device further comprises an outer cover, and the outer cover is detachably covered on the nozzle to cover the rubber head.
Further, the outer cover and the test tube are connected in a buckling mode.
Further, the outer cap and the test tube are made of PP material.
The utility model adopts the above technical scheme, the beneficial effect who has is: the utility model discloses left out the operating procedure who absorbs the sample with the pipettor, reduced the pollution risk that brings in the operation, simplified instrument's use, improved the detection efficiency of timely diagnosis, reduced human cost and protective articles cost.
Drawings
To further illustrate the embodiments, the present invention provides the accompanying drawings. The accompanying drawings, which are incorporated in and constitute a part of this disclosure, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the embodiments. With these references, one of ordinary skill in the art will appreciate other possible embodiments and advantages of the present invention. The components in the drawings are not necessarily to scale, and similar reference numerals are generally used to identify similar components.
FIG. 1 is a schematic diagram of a nucleic acid releasing sampling device according to the present invention;
FIG. 2 is a cross-sectional view of the test tube of the nucleic acid release sampling device shown in FIG. 1;
FIG. 3 is a schematic view of the inner lid and the sampling pipette of the nucleic acid discharge sampling device shown in FIG. 1.
Detailed Description
The present invention will now be further described with reference to the accompanying drawings and detailed description.
As shown in FIGS. 1 to 3, a nucleic acid releasing and sampling apparatus may include a test tube 1, an inner cap 2 and a pipette 3, wherein the test tube 1 contains a nucleic acid releasing agent 100, and a surfactant component and other chemical components such as sodium chloride, Tris-HCl, EDTA and the like contained in the nucleic acid releasing agent 100 are mixed in different ratios to release sample nucleic acids. The inner cap 2 is detachably and hermetically mounted on the nozzle of the test tube 1. The sampling dropper 3 is hermetically inserted into the inner cap 2, and the tube body 31 thereof extends into the test tube 1 while the rubber head 32 is exposed outside the inner cap 1. When the device is used, the inner cover 2 is firstly opened, a sample to be extracted is placed into the test tube 1, the inner cover 2 is screwed immediately, the sample and the nucleic acid releasing agent 100 in the tube body are fully and uniformly mixed (for example, shaking for 10-30S), and finally, the sample can be quantitatively absorbed by the sampling dropper 3 according to the experimental needs and is detected on a machine, so that the operation step of absorbing the sample by a pipettor is omitted, the pollution risk brought by the operation is reduced, the use of instrument equipment is simplified, the detection efficiency of timely diagnosis is improved, and the labor cost and the cost of protective articles are reduced.
Preferably, the inner cap 2 and the test tube 1 are connected in a sealing manner through a thread fit. Specifically, the nozzle of the test tube 1 has an internal thread 11, and the inner cap 2 has an external thread 21. It should be understood that the inner cap 2 and the test tube 1 can be connected in a sealing manner by other means, for example, the inner cap 2 can be a rubber plug with a wide top and a narrow bottom, so as to be fixed on the nozzle of the test tube 1 by plugging.
In this embodiment, the nucleic acid releasing and sampling device may further include an outer cap 4, and the outer cap 4 detachably covers the nozzle of the cuvette 1 to cover the inner cap 2 and the rubber head 31 of the sampling pipette 3, thereby protecting them. Preferably, the outer cover 4 is connected with the test tube 1 by a snap-fit manner. Specifically, the outer cover 4 is provided with a hook 41, and the outer wall of the test tube 1 is provided with a clamping ring 12. Of course, the outer cap 4 may be reversed on the test tube 1 by interference fit.
The material of the test tube 1 and the outer cover 4 can be selected from food-grade PP (Polypropylene plastic) or other materials meeting medical requirements.
The distance from the end (lower end) of the pipette 3 to the bottom of the tube 1 determines the practicality of actual aspiration of the sample. Preferably, the tip of the pipette 3 is located at a distance of 0-0.5cm from the bottom of the test tube 1 to avoid the situation that the sample cannot be aspirated due to the small amount of sample when the sample is aspirated.
As shown in fig. 3, the inner cap 2 and the pipette 3 are integrated for convenience of operation. The sample dropper 3 includes a tube body 31 and a rubber head 32, and a certain amount of a sample can be sucked by pressing the rubber head 32. Preferably, the tube 31 is a glass tube, and a scale 311 is provided thereon to achieve quantitative aspiration of the sample. It should be understood that the tube 31 can be made of different materials such as polypropylene (PP), Polystyrene (PS) and so on. The scale 311 has a precision of 1ul to meet various experimental requirements for different sample sizes. In this embodiment, the tube of the tube 31 decreases gradually towards the end, i.e. the tube 31 is a conical tube, so as to facilitate the suction.
The following is a brief description of the working principle of the present invention, at first, open the outer cover 4, then unscrew the inner cover 2, the user will wait for the sample to be extracted to add into the test tube 1 containing the nucleic acid releasing agent 100, after screwing the inner cover 2, cover the outer cover 4, vibrate 10-30S, and then can quantitatively absorb the required sample amount through the sampling dropper 3 to perform PCR detection or isothermal amplification detection. The whole operation process is very simple and convenient, and the risk of sample pollution is avoided.
While the invention has been particularly shown and described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. The nucleic acid release sampling device is characterized by comprising a test tube, an inner cover and a sampling dropper, wherein the test tube is used for containing a sample and a nucleic acid release agent, the inner cover is detachably and hermetically mounted on a tube opening of the test tube, the sampling dropper is hermetically arranged in the inner cover in a penetrating way, and a tube body of the sampling dropper extends into the test tube while a rubber head is exposed out of the inner cover.
2. The nucleic acid release sampling device of claim 1, wherein the nozzle has an internal thread and the internal cap has an external thread.
3. The nucleic acid release sampling device of claim 1, wherein the body of the sampling pipette is a glass tube having graduations provided thereon.
4. The nucleic acid release sampling device of claim 3, wherein the scale has an accuracy of 1 ul.
5. A nucleic acid releasing sampling device according to claim 1, 3 or 4 wherein the bore of the sampling pipette tapers off towards the distal end.
6. The nucleic acid release sampling device of claim 1, wherein the tip of the sample dropper is less than 0.5cm from the bottom of the tube.
7. The nucleic acid-releasing sampling device according to claim 1, further comprising an outer cap detachably covering the nozzle to cover the rubber head.
8. The nucleic acid release sampling device of claim 7, wherein the outer cover is snap-fit to the test tube.
9. The nucleic acid releasing sampling device according to claim 7, wherein said outer cover and said test tube are made of PP material.
10. The nucleic acid release sampling device of claim 1, wherein the inner lid and the sample dropper are integrated.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121729338.2U CN217359144U (en) | 2021-07-28 | 2021-07-28 | Nucleic acid release sampling device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121729338.2U CN217359144U (en) | 2021-07-28 | 2021-07-28 | Nucleic acid release sampling device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217359144U true CN217359144U (en) | 2022-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121729338.2U Active CN217359144U (en) | 2021-07-28 | 2021-07-28 | Nucleic acid release sampling device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217359144U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119366972A (en) * | 2024-10-30 | 2025-01-28 | 中国人民解放军总医院第三医学中心 | A specimen collection device for pathology department |
-
2021
- 2021-07-28 CN CN202121729338.2U patent/CN217359144U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN119366972A (en) * | 2024-10-30 | 2025-01-28 | 中国人民解放军总医院第三医学中心 | A specimen collection device for pathology department |
| CN119366972B (en) * | 2024-10-30 | 2025-07-15 | 中国人民解放军总医院第三医学中心 | A specimen collection device for pathology department |
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