CN219044930U - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
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- CN219044930U CN219044930U CN202223392369.0U CN202223392369U CN219044930U CN 219044930 U CN219044930 U CN 219044930U CN 202223392369 U CN202223392369 U CN 202223392369U CN 219044930 U CN219044930 U CN 219044930U
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Abstract
The utility model discloses a nucleic acid extraction device. The nucleic acid extraction device comprises a sample extraction matrix having at least one sample collection aperture extending therethrough in a selected direction; an enrichment pad, at least part of which is arranged in the sample collection hole and surrounds the sample collection hole to form a containing chamber capable of containing cell lysate, and the enrichment pad can allow the liquid in the cell lysate to permeate and retain nucleic acid in the cell lysate; and the first water absorption pad is detachably combined with the sample extraction matrix, and when the first water absorption pad is close to and combined with the sample extraction matrix, the first water absorption pad can be contacted with the enrichment pad and absorb redundant liquid on the enrichment pad. The utility model has simple structure, easy manufacture, few operation steps, short time consumption in the extraction process and no assistance of other experimental equipment.
Description
Technical Field
The utility model particularly relates to a nucleic acid extraction device, and belongs to the technical field of nucleic acid detection equipment.
Background
Nucleic acid detection for DNA/RNA has important value in the fields of medical care, personal health, environment, agriculture and the like. In the process of nucleic acid detection, how to extract nucleic acid from biological samples rapidly is critical to detection time, sensitivity, environmental suitability and the like. This is because on the one hand nucleic acids are often very small in biological samples. For cells, there is only one copy of the nucleic acid, and how to capture it efficiently and add it to subsequent amplification systems is a challenge. Another difficulty is how to perform nucleic acid extraction quickly and easily. In general, nucleic acid detection is carried out by using an amplification system based on a nucleic acid polymerase, which is relatively complex in composition and is easily inactivated by adding other substances to a receptor system. For example, phenol chloroform reagents are often used for nucleic acid extraction, and phenol chloroform also deactivates the nucleic acid polymerase, thus requiring multiple washes during the extraction process. On the one hand, the extraction process is complex, the operation time is long, the mistakes are easy to occur, on the other hand, the reagent consumption is increased, the extraction cost is increased, and the extracted nucleic acid is easy to run off in multiple cleaning.
Existing nucleic acid extraction protocols are largely divided into three categories: solution extraction, centrifugation, and magnetic bead methods. The solution extraction method is a classical nucleic acid extraction method, in which nucleic acid is dissolved in an aqueous phase, and proteins are distributed between the aqueous phase and an organic phase, and subjected to centrifugation and washing steps for a plurality of times. The centrifugal column method is that nucleic acid is grabbed on a solid-phase filler column body, protein impurities are removed through washing, and then the nucleic acid is eluted from a main body; the magnetic bead method is to grasp nucleic acid in a solution through the surface groups of the tiny magnetic beads, and then recover the magnetic beads through magnetic force to extract the nucleic acid. However, the solution extraction method involves the use of toxic organic reagents, requires multiple centrifugation and other operations and special laboratory environments and equipment, is cumbersome and long in extraction operation, and although the centrifugal column method can reduce the use of the organic reagents, the centrifugal column method still requires equipment such as a centrifuge, and if liquid elution is carried out by gravity only, the time is greatly prolonged; although the magnetic bead method is simple and convenient, and has been adopted in many cases, the preparation cost of the magnetic beads is high.
Disclosure of Invention
The utility model mainly aims to provide a nucleic acid extraction device which is simple to use, quick to operate, low in cost and convenient to carry, can be used for extracting nucleic acid within a few minutes and detecting the subsequent nucleic acid, and further overcomes the defects in the prior art.
In order to achieve the purpose of the utility model, the technical scheme adopted by the utility model comprises the following steps:
the present utility model provides a nucleic acid extraction device comprising:
a sample extraction substrate having at least one sample collection aperture extending therethrough in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collection hole and is attached to the inner wall of the sample collection hole, the enrichment pad and the sample collection hole are enclosed to form a containing cavity capable of containing cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate and retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction matrix, and when the first water absorption pad is close to and combined with the sample extraction matrix, the first water absorption pad can be contacted with the enrichment pad and absorb redundant liquid on the enrichment pad and residual liquid in the accommodating cavity.
Compared with the prior art, the nucleic acid extraction device provided by the utility model has the advantages of simple structure, easiness in manufacturing, few operation steps, short time consumption in the extraction process and no need of the assistance of other experimental equipment; the nucleic acid extraction device provided by the utility model is small and exquisite, and can be carried outdoors and other places in batches to rapidly extract nucleic acid.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1a is a top view of a nucleic acid isolation apparatus provided in example 1 of the present utility model;
FIG. 1b is a cross-sectional view taken along line A-A of FIG. 1 a;
FIG. 2a is a top view of a nucleic acid isolation apparatus provided in example 2 of the present utility model;
FIG. 2b is a cross-sectional view taken along line A-A in FIG. 2 a;
FIG. 3a is a top view of a nucleic acid isolation apparatus provided in example 3 of the present utility model;
FIG. 3b is a cross-sectional view taken along line A-A in FIG. 3 a;
FIG. 3c is a top view of a first absorbent pad provided in example 3 of the present utility model;
FIG. 3d is a cross-sectional view taken along B-B in FIG. 3 c;
FIG. 4 is a sectional view showing a structure of a nucleic acid isolation apparatus according to example 4 of the present utility model;
FIG. 5 is a sectional view showing a structure of a nucleic acid isolation apparatus according to example 5 of the present utility model;
FIG. 6 is a sectional view showing a structure of a nucleic acid isolation apparatus according to example 6 of the present utility model.
Detailed Description
In view of the shortcomings in the prior art, the inventor of the present utility model has long studied and practiced in a large number of ways to propose the technical scheme of the present utility model. The technical scheme, the implementation process, the principle and the like are further explained as follows.
The present utility model provides a nucleic acid extraction device comprising:
a sample extraction substrate having at least one sample collection aperture extending therethrough in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collection hole and is attached to the inner wall of the sample collection hole, the enrichment pad and the sample collection hole are enclosed to form a containing cavity capable of containing cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate and retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction matrix, and when the first water absorption pad is close to and combined with the sample extraction matrix, the first water absorption pad can be contacted with the enrichment pad and absorb redundant liquid on the enrichment pad and residual liquid in the accommodating cavity.
Further, the enrichment pad is detachably matched with the sample collection hole, and the enrichment pad can be separated from the sample collection hole under the action of external force.
Further, the inner diameter of the first opening of the sample collection well is greater than the inner diameter of the second opening, the maximum outer diameter of the enrichment pad is greater than the inner diameter of the second opening and less than the inner diameter of the first opening, and the enrichment pad is defined to be capable of being pulled out only from the first opening.
Furthermore, the sample collection hole is a conical hole, and specifically, the sample collection hole can be in a truncated cone-shaped structure, namely, a cone without a tip.
Further, the enrichment pad is a conical structure matched with the sample collection hole.
Further, the enrichment pad is of a truncated cone-shaped structure.
Further, all of the enrichment pad is disposed within the sample collection well.
Further, at least a local area of the surface of the enrichment pad is exposed from the second opening, and the surface of the enrichment pad is flush with the plane where the second opening is located, or all of the enrichment pad is located at one side of the plane where the second opening is located, which is close to the first opening.
Further, the first water absorbing pad is further provided with a protruding structure, and the protruding structure can extend into the sample collecting hole from the second opening and contact with the enrichment pad.
In some more specific embodiments, a second water absorbing pad is further disposed in the sample collection hole, the second water absorbing pad is disposed on one side of the enrichment pad near the second opening along the axial direction of the sample collection hole, the second water absorbing pad contacts with the enrichment pad, and the surface of the second water absorbing pad is flush with the plane where the second opening is located, or a part of the second water absorbing pad is exposed outside the sample collection hole from the second opening and contacts with the first water absorbing pad.
Further, a part of the enrichment pad is arranged in the sample collection hole, and the other part of the enrichment pad is exposed outside the sample collection hole from the second opening.
Further, a third water absorption pad is further arranged in the sample collection hole, and the third water absorption pad is arranged on one side, close to the first opening, of the enrichment pad along the axial direction of the sample collection hole.
Further, a plurality of sample collection holes are formed in the sample extraction substrate, and the first water absorption pad can cover the plurality of sample collection holes at the same time and is in contact with the plurality of enrichment pads located in the sample collection holes.
Further, the enrichment pad is a membrane or paper-based card that can bind nucleic acids.
The technical solution, implementation process and principle thereof, etc. will be further explained with reference to the drawings and specific embodiments, and it should be noted that, in the embodiments of the present utility model, the structure of the nucleic acid extraction device is intended to be explained, and unless otherwise specified, the materials of the enrichment pad, the first absorbent pad and the sample extraction substrate used in the embodiments of the present utility model may be materials meeting the requirements of medical equipment, etc., and the specific materials may be known to those skilled in the art.
Example 1
Referring to fig. 1a and 1b, a nucleic acid extraction device includes a sample extraction substrate 100, an enrichment pad 130, and a first absorbent pad 120, wherein a plurality of sample collection holes 110 are formed in the sample extraction substrate 100, the sample collection holes 110 penetrate through the sample extraction substrate 100 along a selected direction, and a first opening 111 and a second opening 112 are formed on the surface of the sample extraction substrate 100; the enrichment pad 130 is integrally disposed in the sample collection hole 110 and encloses with the sample collection hole 110 to form a containing chamber capable of containing a cell lysate, a local area of the surface of the enrichment pad 130 is exposed at the second opening of the sample collection hole 110 and is flush with the second opening, the first absorbent pad 120 is detachably combined with the sample extraction substrate 100, and when the first absorbent pad 120 is close to and combined with the sample extraction substrate 100, the first absorbent pad 120 can contact with a portion of the enrichment pad 130 protruding at the sample collection hole 110 and absorb redundant liquid on the enrichment pad 130 and residual liquid in the containing chamber.
In this embodiment, the enrichment pad 130 is capable of allowing the liquid in the cell lysate to permeate therethrough to retain nucleic acid in the cell lysate, and the enrichment pad 130 is illustratively a membrane or a paper-based card capable of binding nucleic acid, for example, the enrichment pad 130 may be capable of binding nucleic acid PES, PP, PTFE, a nylon membrane, or the like, or the enrichment pad 130 may be an FTA card, or the like.
In this embodiment, the sample extraction substrate 100 may be a sample extraction rod, etc., the sample extraction substrate 100 has a first surface 101 and a second surface 102 opposite to each other along a thickness direction of the sample extraction substrate 100, the sample collection hole 110 penetrates through the sample extraction substrate 100 along the thickness direction, and the first opening 111 of the sample collection hole 110 is located on the first surface 101, and the second opening 112 is located on the second surface 102, where both the first opening 111 and the second opening 112 of the sample collection hole 110 may be circular holes, and an inner diameter of the first opening 111 is greater than an inner diameter of the second opening 112, and the sample collection hole 110 is integrally formed as a truncated cone-shaped structure, i.e. by such a configuration, the inner diameter of the first opening 111 is 3mm, and an inner diameter of the second opening 112 is 2mm.
In this embodiment, the enrichment pad 130 is detachably engaged with the sample collection well 110, and the enrichment pad 130 can be pulled out of the sample collection well 110 by an external force.
In this embodiment, the enrichment pad 130 is integrally in a circular truncated cone structure, specifically, the enrichment pad 130 has a first end face 131 and a second end face 132 opposite to each other along a thickness direction of the enrichment pad 130, where the first end face 131 and the second end face 132 are both circular, and a diameter of the first end face 131 is greater than a diameter of the second end face 132, and a diameter of the first end face 131 is 2.5mm, or the enrichment pad 130 may also be integrally in a cylindrical structure, and the enrichment pad 130 is a flexible member capable of being deformed under an external force.
In this embodiment, the diameter of the first end surface 131 of the enrichment pad 130 (i.e., the outer diameter of the enrichment pad 130 corresponding to the first end surface) is smaller than the inner diameter of the first opening 111, so that the enrichment pad 130 can enter and exit the sample collection well 110 from the first opening 111, the diameter of the first end surface 131 of the enrichment pad 130 is larger than the inner diameter of the second opening 112, and the diameter of the second end surface 132 is equal to the inner diameter of the second opening 112, which allows the enrichment pad 130 to be limited in the sample collection well as to contact the first absorbent pad outside the sample collection well, and the side surface of the enrichment pad 130 is attached to the inner wall of the sample collection well 110, so that the cell lysate in the sample collection well 110 can leak only through the enrichment pad 130.
In this embodiment, the first absorbent pad 120 is detachably disposed on the second surface of the sample extraction substrate 100 and contacts the second end surface 132 of the enrichment pad 130, and the liquid in the sample collection well is driven by capillary action between the first absorbent pad 120 and the enrichment pad 130 to permeate the enrichment pad 130 and be absorbed onto the first absorbent pad 120, while the nucleic acid is trapped on the enrichment pad 130, wherein the first absorbent pad 120 may be a commercially available absorbent paper or the like.
In this embodiment, the use of the nucleic acid extraction apparatus may include: dripping the cell lysate containing nucleic acid into the sample collection hole, enabling the first water absorption pad to be in contact with the enrichment pad, and enabling the liquid in the sample collection hole to be absorbed by the first water absorption pad under the driving of capillary action, wherein the nucleic acid is trapped on the enrichment pad; then adding water or cleaning solution without target nucleic acid into the sample collection hole to wash out excessive impurities on the enrichment pad, sucking excessive water on the enrichment pad by using a dry first water absorption pad, pushing the enrichment pad out of the sample collection hole by using a rod or a protrusion, and pouring the enrichment pad into a container containing a nucleic acid amplification reagent, thus carrying out subsequent experiments such as PCR, LAMP isothermal amplification and the like.
The method for extracting nucleic acid by using the nucleic acid extraction device comprises the following steps: after a disposable sampling swab is smeared in an oral cavity, the disposable sampling swab is soaked in 1mL of RIPA cell lysate (the lysate is required to be added for cell lysis before the sample is added, the common lysate comprises a solution containing SDS, triton, NP-40 and other strong electrolytes or surfactants), then the cell lysate is dripped into a sample collection hole on a sample extraction substrate by a disposable straw, an enrichment pad in the sample collection hole is contacted with the first water absorption pad, the liquid in the sample collection hole is sucked dry, nucleic acid is trapped on the enrichment pad, then the cell lysate (sample loading) can be added into the sample collection hole again to increase the quantity of the enriched nucleic acid, if the quantity of the enriched nucleic acid is enough to be expected, TE buffer is dripped into the sample collection hole again to clean residual impurities on the enrichment pad, then the first water absorption pad is removed and another dry first water absorption pad is replaced to suck the water on the sample collection hole and the enrichment pad; finally, the enrichment pad is pushed out of the sample collection hole by a rod or a protrusion and falls into a reaction tube containing a nucleic acid amplification reagent for subsequent nucleic acid amplification detection.
Example 2
Referring to fig. 2a and 2b, a nucleic acid extraction device includes a sample extraction substrate 200, an enrichment pad 230, and a first absorbent pad 220, wherein the sample extraction substrate 200 has a plurality of sample collection holes 210 therein, the sample collection holes 210 penetrate through the sample extraction substrate 200 along a selected direction, and a first opening 211 and a second opening 212 are formed on the surface of the sample extraction substrate 200; a portion of the enrichment pad 230 is disposed in the sample collection hole 210, and encloses with the sample collection hole 210 to form a receiving chamber capable of receiving a cell lysate, another portion of the enrichment pad is exposed outside the sample collection hole 210, the first absorbent pad 220 is detachably combined with the sample extraction substrate 200, and when the first absorbent pad 220 is close to and combined with the sample extraction substrate 200, the first absorbent pad 220 can contact with the portion of the enrichment pad 230 exposed outside the sample collection hole 210 and absorb the redundant liquid on the enrichment pad 230 and the residual liquid in the receiving chamber.
In this embodiment, the enrichment pad 230 is capable of allowing the liquid in the cell lysate to permeate therethrough to retain nucleic acid in the cell lysate, and the enrichment pad 230 is illustratively a membrane or paper-based card capable of binding nucleic acid, for example, the enrichment pad 230 may be a membrane capable of binding nucleic acid PES, PP, PTFE, nylon, etc., or the enrichment pad 230 may be an FTA card, etc.
In this embodiment, the sample extraction substrate 200 may be a sample extraction rod, etc., the sample extraction substrate 200 has a first surface 201 and a second surface 202 opposite to each other along the thickness direction of the sample extraction substrate 200, the sample collection hole 210 penetrates through the sample extraction substrate 200 along the thickness direction, and the first opening 211 of the sample collection hole 210 is located on the first surface 201, and the second opening 212 is located on the second surface 202, where both the first opening 211 and the second opening 212 of the sample collection hole 210 may be circular holes, and the inner diameter of the first opening 211 is greater than the inner diameter of the second opening 212, and the sample collection hole 210 is integrally in a truncated cone structure, i.e. a taper hole without a tip.
In this embodiment, the enrichment pad 230 is detachably engaged with the sample collection well 210, and the enrichment pad 230 can be pulled out of the sample collection well 210 by an external force.
In this embodiment, the enrichment pad 230 is integrally in a circular truncated cone structure, specifically, the enrichment pad 230 has a first end surface 231 and a second end surface 232 opposite to each other along a thickness direction of the enrichment pad 230, where the first end surface 231 and the second end surface 232 are both circular, and a diameter of the first end surface 231 is greater than a diameter of the second end surface 232, and a diameter of the first end surface 231 is 2.5mm, or the enrichment pad 230 may also be integrally in a cylindrical structure, and the enrichment pad 230 is a flexible member capable of deforming under an external force.
In this embodiment, the diameter of the first end surface 231 of the enrichment pad 230 (i.e., the outer diameter of the enrichment pad 230 corresponding to the first end surface) is smaller than the inner diameter of the first opening 211, so that the enrichment pad 230 can enter and exit the sample collection well 210 from the first opening 211, the diameter of the first end surface 231 of the enrichment pad 230 is larger than the inner diameter of the second opening 212, and the diameter of the second end surface 232 is smaller than the inner diameter of the second opening 212, which enables a portion of the enrichment pad 230 near the second end surface 232 to pass through the second opening and be exposed outside the sample collection well, a portion near the first end surface 231 can be limited in the sample collection well, and the side surface of the enrichment pad 230 is attached to the inner wall of the sample collection well 210, so that the cell lysate in the sample collection well 210 can only leak through the enrichment pad 230.
In this embodiment, the first absorbent pad 220 is detachably disposed on the second surface of the sample extraction substrate 200 and contacts the second end surface 232 of the enrichment pad 230, and the liquid in the sample collection well is driven by capillary action between the first absorbent pad 220 and the enrichment pad 230 to permeate the enrichment pad 230 and be absorbed onto the first absorbent pad 220, while the nucleic acid is trapped on the enrichment pad 230, wherein the first absorbent pad 220 may be a commercially available absorbent paper or the like.
In this embodiment, the first absorbent pad 220 is provided with a plurality of grooves, and the portion of the enrichment pad 230 protruding outside the sample collection hole is correspondingly embedded in the grooves and contacts with the walls of the grooves.
Example 3
Referring to fig. 3a, 3b, 3c and 3d, a nucleic acid extraction device includes a sample extraction substrate 300, an enrichment pad 330 and a first absorbent pad 320, wherein the sample extraction substrate 300 has a plurality of sample collection holes 310 therein, the sample collection holes 310 penetrate the sample extraction substrate 300 along a selected direction, and a first opening 311 and a second opening 312 are formed on the surface of the sample extraction substrate 300; the enrichment pad 330 is integrally disposed in the sample collection hole 310 and encloses with the sample collection hole 310 to form a containing chamber capable of containing a cell lysate, a local area of the surface of the enrichment pad 330 is exposed at the second opening of the sample collection hole 310 and is flush with the second opening, the first absorbent pad 320 is detachably combined with the sample extraction substrate 300, and when the first absorbent pad 320 is close to and combined with the sample extraction substrate 300, the first absorbent pad 320 can contact with a portion of the enrichment pad 330 protruding at the sample collection hole 310 and absorb redundant liquid on the enrichment pad 330 and residual liquid in the containing chamber.
In this embodiment, the enrichment pad 330 is capable of allowing the liquid in the cell lysate to permeate therethrough to retain nucleic acid in the cell lysate, and the enrichment pad 330 is illustratively a membrane or paper-based card capable of binding nucleic acid, for example, the enrichment pad 330 may be capable of binding nucleic acid PES, PP, PTFE, nylon membrane, etc., or the enrichment pad 330 may be an FTA card, etc.
In this embodiment, the sample extraction substrate 300 may be a sample extraction rod, etc., the sample extraction substrate 300 has a first surface 301 and a second surface 302 opposite to each other along a thickness direction thereof, the sample collection hole 310 penetrates through the sample extraction substrate 300 along the thickness direction, and the first opening 311 of the sample collection hole 310 is located on the first surface 301, and the second opening 312 is located on the second surface 302, where both the first opening 311 and the second opening 312 of the sample collection hole 310 may be circular holes, and an inner diameter of the first opening 311 is greater than an inner diameter of the second opening 312, and the sample collection hole 310 is integrally in a truncated cone structure, i.e. a taper hole without a tip.
In this embodiment, the enrichment pad 330 is detachably engaged with the sample collection well 310, and the enrichment pad 330 can be pulled out of the sample collection well 310 by an external force.
In this embodiment, the enrichment pad 330 is integrally in a circular truncated cone structure, specifically, the enrichment pad 330 has a first end face 331 and a second end face 332 disposed opposite to each other along a thickness direction of the enrichment pad 330, where the first end face 331 and the second end face 332 are both circular, and a diameter of the first end face 331 is greater than that of the second end face 332, and a diameter of the first end face 331 is 2.5mm, or the enrichment pad 330 may also be integrally in a cylindrical structure, and the enrichment pad 330 is a flexible member capable of being deformed under an external force.
In this embodiment, the diameter of the first end surface 331 of the enrichment pad 330 (i.e., the outer diameter of the enrichment pad 330 corresponding to the first end surface) is smaller than the inner diameter of the first opening 311, so that the enrichment pad 330 can enter and exit the sample collection hole 310 from the first opening 311, while the diameter of the first end surface 331 of the enrichment pad 330 is larger than the inner diameter of the second opening 312, and the diameter of the second end surface 332 is larger than the inner diameter of the second opening 312, which enables the enrichment pad 330 to be confined in the sample collection hole as a whole, and meanwhile, a distance is provided between the second end surface 332 of the enrichment pad 330 and the second opening 312 of the sample collection hole.
In this embodiment, the side of the enrichment pad 330 is attached to the inner wall of the sample collection well 310, so that the cell lysate in the sample collection well 310 can only leak through the enrichment pad 330.
In this embodiment, the first absorbent pad 320 is further provided with a plurality of protruding structures 321, each protruding structure 321 corresponds to a sample collection hole 310, and the protruding structures 321 can extend into the sample collection hole 310 from the second opening 312 and contact with the enrichment pad 330.
In this embodiment, the first absorbent pad 320 is detachably disposed on the second surface of the sample extraction substrate 300 and contacts the second end surface 332 of the enrichment pad 330, and the liquid in the sample collection well is driven by capillary action between the first absorbent pad 320 and the enrichment pad 330 to permeate the enrichment pad 330 and be absorbed onto the first absorbent pad 320, while the nucleic acid is trapped on the enrichment pad 330, wherein the first absorbent pad 320 may be a commercially available absorbent paper or the like.
Example 4
Referring to fig. 4, the structure of a nucleic acid isolation apparatus in this embodiment is substantially the same as that of embodiment 3, and the same parts will not be described here again, and the difference between embodiment 4 and embodiment 3 is that: the first absorbent pad 320 in this embodiment is not provided with a protruding structure, but is flat, the sample collection hole 310 is further provided with a second absorbent pad 400, the second absorbent pad 400 is disposed on a side of the enrichment pad 330 near the second opening 312 along the axial direction of the sample collection hole 310, the second absorbent pad 400 is in contact with the enrichment pad 330, and the surface of the second absorbent pad 400 is flush with the plane of the second opening 312, or a part of the second absorbent pad 400 is exposed outside the sample collection hole 310 from the second opening 310, so as to be in contact with the first absorbent pad 320.
In this embodiment, the second water absorbent pad 400 does not adsorb nucleic acid, but absorbs water by capillary action and guides the water to the first water absorbent pad 320, wherein the second water absorbent pad 400 may be a general filter paper.
Example 5
Referring to fig. 5, the structure of a nucleic acid isolation apparatus in this embodiment is substantially the same as that of embodiment 2, and the same parts will not be described here again, and the difference between embodiment 5 and embodiment 2 is that: the sample collection well 210 is further provided with a third water absorbent pad 500, the third water absorbent pad 500 is disposed on one side of the enrichment pad 230 near the first opening 211 along the axial direction of the sample collection well 210, the third water absorbent pad 500 does not absorb nucleic acid, but absorbs water by capillary action, and can filter and intercept large-fragment impurities, wherein the third water absorbent pad 500 can be a common filter paper.
Example 6
Referring to fig. 6, the structure of a nucleic acid isolation apparatus in this embodiment is substantially the same as that of embodiment 4, and the same parts will not be described here again, and the difference between embodiment 6 and embodiment 4 is that: the sample collection well 310 is further provided with a third water absorbent pad 500, the third water absorbent pad 500 is disposed on one side of the enrichment pad 330 near the first opening 311 along the axial direction of the sample collection well 310, the third water absorbent pad 500 does not absorb nucleic acid, but absorbs water by capillary action, and can filter and intercept large-fragment impurities, wherein the third water absorbent pad 500 can be a common filter paper.
The nucleic acid extraction device provided by the utility model has the advantages of simple structure and low manufacturing cost, complicated operation process is not needed when the device is used, and large-scale experimental devices such as a centrifugal machine and the like are not needed, so that the nucleic acid in a sample can be extracted and enriched within a few minutes and used for detecting the nucleic acid, the nucleic acid detection is easy to carry out, and the device is not limited by laboratory conditions.
The nucleic acid extraction device provided by the utility model has the advantages of simple structure, easiness in manufacturing, few operation steps, short time consumption in the extraction process and no need of assistance of other experimental equipment; the nucleic acid extraction device provided by the utility model is small and exquisite, can be carried outdoors and the like in batches, is convenient and rapid to extract nucleic acid, and provides a proper enrichment sample for subsequent nucleic acid amplification detection.
It should be understood that the above embodiments are merely for illustrating the technical concept and features of the present utility model, and are intended to enable those skilled in the art to understand the present utility model and implement the same according to the present utility model without limiting the scope of the present utility model. All equivalent changes or modifications made in accordance with the spirit of the present utility model should be construed to be included in the scope of the present utility model.
Claims (10)
1. A nucleic acid extraction device, comprising:
a sample extraction substrate having at least one sample collection aperture extending therethrough in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collection hole and is attached to the inner wall of the sample collection hole, the enrichment pad and the sample collection hole are enclosed to form a containing cavity capable of containing cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate and retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction matrix, and when the first water absorption pad is close to and combined with the sample extraction matrix, the first water absorption pad can be contacted with the enrichment pad and absorb redundant liquid on the enrichment pad and residual liquid in the accommodating cavity.
2. The nucleic acid extraction device according to claim 1, wherein: the enrichment pad is detachably matched with the sample collection hole, and the enrichment pad can be separated from the sample collection hole under the action of external force.
3. The nucleic acid extraction device according to claim 2, wherein: the inner diameter of the first opening of the sample collection well is greater than the inner diameter of the second opening, the maximum outer diameter of the enrichment pad is greater than the inner diameter of the second opening and less than the inner diameter of the first opening, and the enrichment pad is limited to be capable of being pulled out from the first opening only; and/or, the sample collection well is a tapered well.
4. The nucleic acid extraction device according to claim 3, wherein: the enrichment pad is of a conical structure matched with the sample collection hole; and/or the enrichment pad is in a truncated cone-shaped structure.
5. The nucleic acid extraction device according to claim 3, wherein: all the enrichment pads are arranged in the sample collection holes; and/or, at least a local area of the surface of the enrichment pad is exposed from the second opening, and the surface of the enrichment pad is flush with the plane where the second opening is located, or all of the enrichment pad is located at one side, close to the first opening, of the plane where the second opening is located.
6. The nucleic acid extraction apparatus according to claim 5, wherein: the first water absorption pad is also provided with a protruding structure, and the protruding structure can extend into the sample collection hole from the second opening and contact with the enrichment pad.
7. The nucleic acid extraction apparatus according to claim 5, wherein: the sample collection hole is internally provided with a second water absorption pad, the second water absorption pad is arranged on one side, close to the second opening, of the enrichment pad along the axial direction of the sample collection hole, the second water absorption pad is in contact with the enrichment pad, and the surface of the second water absorption pad is flush with the plane where the second opening is located, or part of the second water absorption pad is exposed outside the sample collection hole from the second opening, and is in contact with the first water absorption pad.
8. The nucleic acid extraction device according to claim 3, wherein: one part of the enrichment pad is arranged in the sample collection hole, and the other part of the enrichment pad is exposed outside the sample collection hole from the second opening.
9. The nucleic acid extraction device according to any one of claims 1 to 8, wherein: and a third water absorption pad is further arranged in the sample collection hole, and the third water absorption pad is arranged on one side, close to the first opening, of the enrichment pad along the axial direction of the sample collection hole.
10. The nucleic acid extraction device according to claim 1, wherein: the first water absorption pad can cover a plurality of sample collection holes at the same time and is in contact with a plurality of enrichment pads positioned in the sample collection holes; and/or, the enrichment pad is a membrane or paper-based card that can bind nucleic acids.
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| CN202223392369.0U CN219044930U (en) | 2022-12-13 | 2022-12-13 | Nucleic acid extraction device |
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| CN202223392369.0U CN219044930U (en) | 2022-12-13 | 2022-12-13 | Nucleic acid extraction device |
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