CN115786078A - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
- Publication number
- CN115786078A CN115786078A CN202211631536.4A CN202211631536A CN115786078A CN 115786078 A CN115786078 A CN 115786078A CN 202211631536 A CN202211631536 A CN 202211631536A CN 115786078 A CN115786078 A CN 115786078A
- Authority
- CN
- China
- Prior art keywords
- pad
- enrichment
- sample
- opening
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a nucleic acid extraction device. The nucleic acid extraction device includes a sample extraction matrix having at least one sample collection well extending through the sample extraction matrix in a selected direction; the enrichment pad is at least partially arranged in the sample collecting hole and forms a containing chamber capable of containing cell lysate with the sample collecting hole in an enclosing manner, and the enrichment pad can enable liquid in the cell lysate to permeate to retain nucleic acid in the cell lysate; a first bibulous pad releasably engageable with the sample extraction matrix and capable of contacting the enrichment pad and absorbing excess fluid therefrom when the first bibulous pad is brought into proximity with and engaged with the sample extraction matrix. The invention has simple structure, easy manufacture, few operation steps, short time consumption in the extraction process and no need of the assistance of other experimental equipment.
Description
Technical Field
The invention particularly relates to a nucleic acid extraction device, and belongs to the technical field of nucleic acid detection equipment.
Background
Nucleic acid detection for DNA/RNA has important value in the fields of medical health, personal health, environment, agriculture and the like. In the nucleic acid detection process, how to rapidly extract nucleic acid from a biological sample is very critical to detection time, sensitivity, environmental applicability and the like. This is because on the one hand nucleic acids are often very small in biological samples. For cells, there is only one copy of nucleic acid, and it is a challenge to capture and add it to the subsequent amplification system with high efficiency. Another difficulty is how to perform nucleic acid extraction quickly and easily. In general, nucleic acid detection is performed by using an amplification system based on nucleic acid polymerase, which has relatively complex components and is easily inactivated by other substances added to the nucleic acid polymerase. For example, the nucleic acid extraction often uses phenol chloroform, which also deactivates the nucleic acid polymerase, thus requiring multiple washes during the extraction process. On one hand, the extraction process is complex, the operation time is long, errors are prone to occur, on the other hand, reagent consumption is increased, the extraction cost is increased, and the extracted nucleic acid is prone to loss in multiple times of cleaning.
The existing nucleic acid extraction schemes are mainly divided into three categories: solution extraction, centrifugal column, and magnetic bead methods. The solution extraction method is a classical nucleic acid extraction method, and utilizes the steps of dissolving nucleic acid in water phase, distributing protein between water phase and organic phase, and centrifuging and washing for several times. The centrifugal column method comprises the steps of grabbing nucleic acid on a solid-phase filler column, removing protein impurities through washing, and then eluting the nucleic acid from a main body; the magnetic bead method is to grab nucleic acid in solution through the surface groups of tiny magnetic beads, and then to recover the magnetic beads through magnetic force, so as to realize the extraction of the nucleic acid. However, the solution extraction method involves the use of toxic organic reagents, and requires multiple operations such as centrifugation and special laboratory environment and equipment, and the extraction operation is complicated and long-lasting, and although the centrifugal column method can reduce the use of organic reagents, equipment such as a centrifuge is still required, and if liquid elution is performed only by gravity, the time is greatly prolonged; although the magnetic bead method is simple and has been widely used, the magnetic bead preparation cost is high.
Disclosure of Invention
The invention mainly aims to provide a nucleic acid extraction device which is simple to use, quick to operate, low in cost and convenient to carry, can extract nucleic acid within minutes and is used for subsequent nucleic acid detection, so that the defects in the prior art are overcome.
In order to achieve the purpose, the technical scheme adopted by the invention comprises the following steps:
the present invention provides a nucleic acid extraction apparatus, comprising:
a sample extraction substrate having at least one sample collection well extending through the sample extraction substrate in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collecting hole and is attached to the inner wall of the sample collecting hole, the enrichment pad and the sample collecting hole are enclosed to form an accommodating chamber capable of accommodating cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate through to retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction matrix, and when the first water absorption pad is close to and combined with the sample extraction matrix, the first water absorption pad can be contacted with the enrichment pad and absorb the redundant liquid on the enrichment pad and the residual liquid in the containing chamber.
Compared with the prior art, the nucleic acid extraction device provided by the invention has the advantages of simple structure, easiness in manufacturing, few operation steps, short extraction process time consumption and no need of assistance of other experimental equipment; the nucleic acid extraction device provided by the invention is small and exquisite, and can be carried to outdoor places and other places to rapidly extract nucleic acid in batches.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the description below are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1a is a top view of a nucleic acid isolation apparatus provided in example 1 of the present invention;
FIG. 1b isbase:Sub>A cross-sectional view taken along A-A of FIG. 1base:Sub>A;
FIG. 2a is a top view of a nucleic acid isolation apparatus provided in example 2 of the present invention;
FIG. 2b isbase:Sub>A cross-sectional view taken along A-A of FIG. 2base:Sub>A;
FIG. 3a is a top view of a nucleic acid isolation apparatus provided in example 3 of the present invention;
FIG. 3b isbase:Sub>A cross-sectional view taken along A-A of FIG. 3base:Sub>A;
FIG. 3c is a top view of a first absorbent pad provided in example 3 of the present invention;
FIG. 3d is a cross-sectional view taken along line B-B of FIG. 3 c;
FIG. 4 is a sectional view of a nucleic acid isolation apparatus provided in example 4 of the present invention;
FIG. 5 is a sectional view of a nucleic acid isolation apparatus provided in example 5 of the present invention;
FIG. 6 is a sectional view of a nucleic acid isolation apparatus according to example 6 of the present invention.
Detailed Description
In view of the defects in the prior art, the inventor of the present invention has made extensive research and practice to propose the technical solution of the present invention. The technical solution, its implementation and principles, etc. will be further explained as follows.
The present invention provides a nucleic acid extraction apparatus, comprising:
a sample extraction substrate having at least one sample collection well extending through the sample extraction substrate in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collecting hole and is attached to the inner wall of the sample collecting hole, the enrichment pad and the sample collecting hole are enclosed to form an accommodating chamber capable of accommodating cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate through to retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction substrate, and can contact with the enrichment pad and absorb redundant liquid on the enrichment pad and liquid remained in the containing chamber when the first water absorption pad is close to and combined with the sample extraction substrate.
Furthermore, the enrichment pad and the sample collection hole are detachably matched, and the enrichment pad can be separated from the sample collection hole under the action of external force.
Further, the inner diameter of the first opening of the sample collection well is larger than the inner diameter of the second opening, the maximum outer diameter of the enrichment pad is larger than the inner diameter of the second opening and smaller than the inner diameter of the first opening, and the enrichment pad is limited to be capable of being pulled out from the first opening only.
Further, the sample collection hole is a tapered hole, and specifically, the sample collection hole may be a truncated cone-shaped structure, i.e., a non-sharp cone.
Further, the enrichment pad is a tapered structure that matches the sample collection well.
Further, the enrichment pad is of a circular truncated cone-shaped structure.
Further, all of the enrichment pad is disposed within the sample collection well.
Furthermore, at least a partial area of the surface of the enrichment pad is exposed from the second opening, and the surface of the enrichment pad is flush with the plane of the second opening, or the enrichment pad is entirely positioned on one side, close to the first opening, of the plane of the second opening.
Furthermore, the first water absorption pad is also provided with a protruding structure, and the protruding structure can extend into the sample collection hole from the second opening and contact with the enrichment pad.
In some specific embodiments, a second absorbent pad is further disposed in the sample collection hole, and the second absorbent pad is disposed on a side of the enrichment pad close to the second opening along the axial direction of the sample collection hole, and the second absorbent pad is in contact with the enrichment pad, and a surface of the second absorbent pad is flush with a plane where the second opening is located, or a part of the second absorbent pad is further exposed outside the sample collection hole from the second opening and is in contact with the first absorbent pad.
Further, a portion of the enrichment pad is disposed within the sample collection well, and another portion protrudes from the second opening outside the sample collection well.
Furthermore, a third water absorption pad is arranged in the sample collection hole and is arranged on one side, close to the first opening, of the enrichment pad along the axial direction of the sample collection hole.
Furthermore, a plurality of sample collecting holes are formed in the sample extraction substrate, and the first water absorption pad can simultaneously cover the plurality of sample collecting holes and is in contact with a plurality of enrichment pads positioned in the sample collecting holes.
Further, the enrichment pad is a membrane or paper-based card that can bind nucleic acids.
In the following, the technical scheme, the implementation process, the principle and the like will be further explained with reference to the accompanying drawings and the specific embodiments, it should be noted that the embodiment of the present invention is intended to explain the structure of the nucleic acid extracting apparatus, and unless otherwise specified, the materials of the enrichment pad, the first water absorption pad and the sample extraction matrix used in the embodiment of the present invention may be materials meeting the requirements of medical equipment, and the specific materials may be known to those skilled in the art.
Example 1
Referring to fig. 1a and 1b, a nucleic acid extraction device includes a sample extraction substrate 100, an enrichment pad 130, and a first water absorption pad 120, wherein the sample extraction substrate 100 has a plurality of sample collection holes 110 therein, the sample collection holes 110 penetrate through the sample extraction substrate 100 along a selected direction, and a first opening 111 and a second opening 112 are formed on a surface of the sample extraction substrate 100; the enrichment pad 130 is integrally disposed in the sample collection hole 110, and forms a containing chamber capable of containing cell lysate with the sample collection hole 110, a local area of the surface of the enrichment pad 130 is exposed at the second opening of the sample collection hole 110 and is flush with the second opening, the first water absorption pad 120 is separably combined with the sample extraction substrate 100, and when the first water absorption pad 120 is close to and combined with the sample extraction substrate 100, the first water absorption pad 120 can contact with a portion of the enrichment pad 130 protruding out of the sample collection hole 110 and absorb the redundant liquid on the enrichment pad 130 and the residual liquid in the containing chamber.
In the present embodiment, the enrichment pad 130 can permeate the liquid in the cell lysate to retain the nucleic acid in the cell lysate, and the enrichment pad 130 is exemplarily a membrane or a paper-based card that can bind the nucleic acid, for example, the enrichment pad 130 can be a membrane capable of binding PES, PP, PTFE, nylon, or the like, or the enrichment pad 130 can be an FTA card or the like.
In this embodiment, the sample extraction base 100 may be a sample extraction rod, the sample extraction base 100 has a first surface 101 and a second surface 102 oppositely arranged along a thickness direction thereof, the sample collection hole 110 penetrates through the sample extraction base 100 along the thickness direction, the first opening 111 of the sample collection hole 110 is located on the first surface 101, the second opening 112 is located on the second surface 102, wherein the first opening 111 and the second opening 112 of the sample collection hole 110 may be circular holes, and an inner diameter of the first opening 111 is larger than an inner diameter of the second opening 112, and the sample collection hole 110 is integrally a truncated cone-shaped structure, i.e., a cone-shaped hole without a tip, and by such arrangement, the sample collection hole 110 is integrally formed into a funnel-shaped structure, illustratively, an inner diameter of the first opening 111 is 3mm, and an inner diameter of the second opening 112 is 2mm.
In this embodiment, the enrichment pad 130 is detachably engaged with the sample collection hole 110, and the enrichment pad 130 can be removed from the sample collection hole 110 by an external force.
In this embodiment, the whole body of the enrichment mat 130 is a circular truncated cone-shaped structure, specifically, the enrichment mat 130 has a first end surface 131 and a second end surface 132 which are oppositely arranged along the thickness direction thereof, wherein the first end surface 131 and the second end surface 132 are both circular, the diameter of the first end surface 131 is larger than that of the second end surface 132, the diameter of the first end surface 131 is 2.5mm, or the whole body of the enrichment mat 130 can also be a cylindrical structure, and the enrichment mat 130 is a flexible member which can deform under the external force.
In this embodiment, the diameter of the first end surface 131 of the enrichment pad 130 (i.e., the outer diameter of the enrichment pad 130 corresponding to the first end surface) is smaller than the inner diameter of the first opening 111, so that the enrichment pad 130 can enter and exit the sample collection hole 110 from the first opening 111, the diameter of the first end surface 13l of the enrichment pad 130 is larger than the inner diameter of the second opening 112, and the diameter of the second end surface 132 is equal to the inner diameter of the second opening 112, so that the entire enrichment pad 130 can be limited in the sample collection hole and can also contact with the first water absorption pad outside the sample collection hole, and the side surface of the enrichment pad 130 is attached to the inner wall of the sample collection hole 110, so that the cell lysate in the sample collection hole 110 can only leak through the enrichment pad 130.
In this embodiment, the first absorbent pad 120 is detachably disposed on the second surface of the sample extraction substrate 100 and contacts with the second end 132 of the enrichment pad 130, and under the driving of the capillary action between the first absorbent pad 120 and the enrichment pad 130, the liquid in the sample collection well permeates through the enrichment pad 130 and is absorbed onto the first absorbent pad 120, while the nucleic acid is trapped on the enrichment pad 130, wherein the first absorbent pad 120 can be commercially available absorbent paper or the like.
In this embodiment, the use of the nucleic acid extraction apparatus may include: dripping cell lysate containing nucleic acid into the sample collection hole, enabling the first water absorption pad to be in contact with the enrichment pad, and driving liquid in the sample collection hole to be absorbed by the first water absorption pad under the driving of capillary action, so that the nucleic acid is trapped on the enrichment pad; and then adding water or cleaning solution without target nucleic acid into the sample collecting hole to wash off redundant impurities on the enrichment pad, absorbing redundant water on the enrichment pad by using a dry first water absorption pad, pushing the enrichment pad out of the sample collecting hole by using a rod or a protrusion, and pouring the enrichment pad into a container containing a nucleic acid amplification reagent, so that subsequent nucleic acid amplification detection, such as PCR, LAMP isothermal amplification and other experiments, can be carried out.
Illustratively, the method for extracting nucleic acid using the nucleic acid extraction apparatus is: after a disposable sampling swab is smeared in an oral cavity, the sampling swab is soaked in 1mL of RIPA cell lysate (the lysate is required to be added for cell lysis before a sample is added, common lysate comprises a solution containing strong electrolyte such as SDS, triton, NP-40 and the like or surfactant), then the cell lysate is dripped into a sample collection hole on a sample extraction substrate by using a disposable suction tube, an enrichment pad in the sample collection hole is contacted with a first water absorption pad, liquid in the sample collection hole is absorbed and dried, nucleic acid is trapped on the enrichment pad, and then the cell lysate (sample application) can be added into the sample collection hole again to improve the quantity of the enriched nucleic acid; and finally, pushing the enrichment pad out of the sample collection hole by using a rod or a protrusion and dropping the enrichment pad into a reaction tube containing a nucleic acid amplification reagent to carry out subsequent nucleic acid amplification detection.
Example 2
Referring to fig. 2a and 2b, a nucleic acid extracting apparatus includes a sample extracting base 200, an enrichment pad 230, and a first water absorption pad 220, wherein the sample extracting base 200 has a plurality of sample collecting holes 210 therein, the sample collecting holes 210 penetrate through the sample extracting base 200 along a selected direction, and a first opening 211 and a second opening 212 are formed on a surface of the sample extracting base 200; a portion of the enrichment pad 230 is disposed inside the sample collection well 210, and surrounds the sample collection well 210 to form a containing chamber capable of containing cell lysate, and another portion protrudes outside the sample collection well 210, the first absorbent pad 220 is detachably combined with the sample extraction matrix 200, and when the first absorbent pad 220 is close to and combined with the sample extraction matrix 200, the first absorbent pad 220 can contact with the portion of the enrichment pad 230 protruding outside the sample collection well 210 and absorb the excess liquid on the enrichment pad 230 and the residual liquid in the containing chamber.
In the present embodiment, the enrichment pad 230 is capable of permeating the liquid in the cell lysate to retain the nucleic acid in the cell lysate, and the enrichment pad 230 is exemplarily a membrane or a paper-based card capable of binding the nucleic acid, for example, the enrichment pad 230 may be a membrane capable of binding the nucleic acid PES, PP, PTFE, nylon, or the like, or the enrichment pad 230 may be an FTA card or the like.
In this embodiment, the sample extraction base 200 may be a sample extraction rod, the sample extraction base 200 has a first face 201 and a second face 202 oppositely arranged along a thickness direction thereof, the sample collection hole 210 penetrates through the sample extraction base 200 along the thickness direction, a first opening 211 of the sample collection hole 210 is located on the first face 201, and a second opening 212 is located on the second face 202, wherein the first opening 211 and the second opening 212 of the sample collection hole 210 may be circular holes, and an inner diameter of the first opening 211 is larger than an inner diameter of the second opening 212, and the sample collection hole 210 is integrally a truncated cone-shaped structure, i.e., a tapered hole without a tip, and by such arrangement, the sample collection hole 210 is integrally formed into a funnel-shaped structure, for example, an inner diameter of the first opening 211 is 3mm, and an inner diameter of the second opening 212 is 2mm.
In this embodiment, the enrichment pad 230 is detachably engaged with the sample collection hole 210, and the enrichment pad 230 can be pulled out from the sample collection hole 210 by an external force.
In this embodiment, the whole body of the enrichment mat 230 is a circular truncated cone-shaped structure, specifically, the enrichment mat 230 has a first end surface 231 and a second end surface 232 which are oppositely arranged along the thickness direction thereof, wherein the first end surface 231 and the second end surface 232 are both circular, the diameter of the first end surface 231 is greater than that of the second end surface 232, the diameter of the first end surface 23l is 2.5mm, or the whole body of the enrichment mat 230 can also be a cylindrical structure, and the enrichment mat 230 is a flexible member which can deform under the action of external force.
In this embodiment, the diameter of the first end surface 231 of the enrichment pad 230 (i.e. the outer diameter of the enrichment pad 230 corresponding to the first end surface) is smaller than the inner diameter of the first opening 211, so that the enrichment pad 230 can enter and exit the sample collection hole 210 from the first opening 211, the diameter of the first end surface 231 of the enrichment pad 230 is larger than the inner diameter of the second opening 212, and the diameter of the second end surface 232 is smaller than the inner diameter of the second opening 212, so that a portion of the enrichment pad 230 close to the second end surface 232 can pass through the second opening and protrude outside the sample collection hole, the portion close to the first end surface 231 can be limited in the sample collection hole, and the side surface of the enrichment pad 230 is attached to the inner wall of the sample collection hole 210, so that the cell lysate in the sample collection hole 210 can only leak through the enrichment pad 230.
In this embodiment, the first absorbent pad 220 is detachably disposed on the second surface of the sample collection substrate 200 and contacts with the second end surface 232 of the enrichment pad 230, and under the driving of the capillary action between the first absorbent pad 220 and the enrichment pad 230, the liquid in the sample collection well permeates through the enrichment pad 230 and is absorbed onto the first absorbent pad 220, while the nucleic acid is trapped on the enrichment pad 230, wherein the first absorbent pad 220 may be commercially available absorbent paper or the like.
In this embodiment, the first absorbent pad 220 is provided with a plurality of grooves, and the portion of the enrichment pad 230 protruding out of the sample collection hole is correspondingly embedded in the grooves and contacts with the walls of the grooves.
Example 3
Referring to fig. 3a, 3b, 3c, and 3d, a nucleic acid extraction device includes a sample extraction substrate 300, an enrichment pad 330, and a first water absorption pad 320, wherein the sample extraction substrate 300 has a plurality of sample collection holes 310 therein, the sample collection holes 310 penetrate through the sample extraction substrate 300 along a selected direction, and a first opening 311 and a second opening 312 are formed on a surface of the sample extraction substrate 300; the enrichment pad 330 is integrally disposed in the sample collection hole 310, and forms a containing chamber capable of containing cell lysate with the sample collection hole 310, a local area of the surface of the enrichment pad 330 is exposed at the second opening of the sample collection hole 310 and is flush with the second opening, the first water absorption pad 320 is detachably combined with the sample extraction substrate 300, and when the first water absorption pad 320 is close to and combined with the sample extraction substrate 300, the first water absorption pad 320 can contact with the part of the enrichment pad 330 protruding out of the sample collection hole 310 and absorb the excess liquid on the enrichment pad 330 and the residual liquid in the containing chamber.
In the present embodiment, the enrichment pad 330 is capable of permeating the liquid in the cell lysate to retain the nucleic acid in the cell lysate, and illustratively, the enrichment pad 330 is a membrane or paper-based card capable of binding the nucleic acid, for example, the enrichment pad 330 may be a membrane capable of binding the nucleic acid PES, PP, PTFE, nylon, or the like, or the enrichment pad 330 may be an FTA card or the like.
In this embodiment, the sample extraction base 300 may be a sample extraction rod, the sample extraction base 300 has a first face 301 and a second face 302 oppositely arranged along a thickness direction thereof, the sample collection hole 310 penetrates through the sample extraction base 300 along the thickness direction, the first opening 311 of the sample collection hole 310 is located on the first face 301, and the second opening 312 is located on the second face 302, wherein the first opening 311 and the second opening 312 of the sample collection hole 310 may be circular holes, the inner diameter of the first opening 311 is larger than the inner diameter of the second opening 312, and the sample collection hole 310 is integrally of a truncated cone-shaped structure, i.e., a cone-shaped hole without a tip, and by such an arrangement, the sample collection hole 310 is integrally formed into a funnel-shaped structure, for example, the inner diameter of the first opening 311 is 3mm, and the inner diameter of the second opening 312 is 2mm.
In the embodiment, the enrichment pad 330 is detachably engaged with the sample collection hole 310, and the enrichment pad 330 can be pulled out from the sample collection hole 310 by an external force.
In this embodiment, the whole enrichment mat 330 is a round platform-shaped structure, specifically, the enrichment mat 330 has the first end surface 331 and the second end surface 332 that set up along self thickness direction back to back, wherein, the first end surface 331 and the second end surface 332 are circular, just the diameter of first end surface 331 is greater than the diameter of second end surface 332, the diameter of first end surface 331 is 2.5mm, or, the whole enrichment mat 330 also can be cylindrical structure, just the enrichment mat 330 is the flexible component that can take place the deformation under the exogenic action.
In this embodiment, the diameter of the first end surface 331 of the enrichment pad 330 (i.e. the outer diameter of the enrichment pad 330 corresponding to the first end surface) is smaller than the inner diameter of the first opening 311, so that the enrichment pad 330 can enter and exit the sample collection hole 310 from the first opening 311, the diameter of the first end surface 331 of the enrichment pad 330 is larger than the inner diameter of the second opening 312, and the diameter of the second end surface 332 is larger than the inner diameter of the second opening 312, so that the entire enrichment pad 330 can be confined in the sample collection hole, and meanwhile, there is a distance between the second end surface 332 of the enrichment pad 330 and the second opening 312 of the sample collection hole.
In this embodiment, the side surface of the enrichment pad 330 is attached to the inner wall of the sample collection well 310, so that the cell lysate in the sample collection well 310 can only leak through the enrichment pad 330.
In this embodiment, the first absorbent pad 320 is further provided with a plurality of protrusion structures 321, each protrusion structure 321 corresponds to a sample collection hole 310, and the protrusion structure 321 can extend into the sample collection hole 310 from the second opening 312 and contact with the enrichment pad 330.
In this embodiment, the first absorbent pad 320 is detachably disposed on the second surface of the sample-extracting substrate 300 and contacts with the second end surface 332 of the enrichment pad 330, and under the driving of the capillary action between the first absorbent pad 320 and the enrichment pad 330, the liquid in the sample collecting hole permeates through the enrichment pad 330 and is absorbed onto the first absorbent pad 320, and the nucleic acid is trapped on the enrichment pad 330, wherein the first absorbent pad 320 may be commercially available absorbent paper or the like.
Example 4
Referring to fig. 4, the structure of a nucleic acid extracting apparatus in this embodiment is substantially the same as that in embodiment 3, and the description of the same parts is omitted here, and the difference between embodiment 4 and embodiment 3 lies in: in this embodiment, the first water absorption pad 320 is not provided with a protrusion structure, but is flat, the second water absorption pad 400 is further provided in the sample collection hole 310, the second water absorption pad 400 is provided on one side of the enrichment pad 330 near the second opening 312 along the axial direction of the sample collection hole 310, the second water absorption pad 400 is in contact with the enrichment pad 330, and the surface of the second water absorption pad 400 is flush with the plane of the second opening 312, or a part of the second water absorption pad 400 is further exposed outside the sample collection hole 310 from the second opening 310 so as to be in contact with the first water absorption pad 320.
In this embodiment, the second absorbent pad 400 does not adsorb nucleic acids, but absorbs water by capillary action and guides the water to the first absorbent pad 320, wherein the second absorbent pad 400 may be ordinary filter paper.
Example 5
Referring to fig. 5, the structure of a nucleic acid extracting apparatus in this embodiment is substantially the same as that in embodiment 2, and the description of the same parts is omitted here, and the difference between embodiment 5 and embodiment 2 lies in: the sample collection hole 210 is further provided with a third absorbent pad 500, the third absorbent pad 500 is disposed on one side of the enrichment pad 230 near the first opening 211 along the axial direction of the sample collection hole 210, the third absorbent pad 500 does not absorb nucleic acid, but absorbs water through capillary action, and can filter and intercept large-fragment impurities, wherein the third absorbent pad 500 can be made of common filter paper.
Example 6
Referring to fig. 6, the structure of a nucleic acid extracting apparatus in this embodiment is substantially the same as that in embodiment 4, and the description of the same parts is omitted here, and the difference between embodiment 6 and embodiment 4 lies in: the sample collection hole 310 is further provided with a third absorbent pad 500, the third absorbent pad 500 is disposed on one side of the enrichment pad 330 near the first opening 311 along the axial direction of the sample collection hole 310, the third absorbent pad 500 does not absorb nucleic acid, but absorbs water through capillary action, and can filter and intercept large-fragment impurities, wherein the third absorbent pad 500 can be made of common filter paper.
The nucleic acid extraction device provided by the invention has the advantages of simple structure and low cost, and can be used for extracting and enriching nucleic acid in a sample within minutes without complicated operation processes and large-scale experimental devices such as a centrifugal machine and the like, so that the nucleic acid detection is easy to carry out and is not limited by laboratory conditions.
The nucleic acid extraction device provided by the invention has the advantages of simple structure, easiness in manufacturing, few operation steps, short extraction process time consumption and no need of assistance of other experimental equipment; the nucleic acid extraction device provided by the invention is small and exquisite, can be carried to outdoor places and other places in batches, is convenient and rapid to extract nucleic acid, and provides a proper enrichment sample for subsequent nucleic acid amplification detection.
It should be understood that the above-mentioned embodiments are merely illustrative of the technical concepts and features of the present invention, which are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and therefore, the protection scope of the present invention is not limited thereby. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. A nucleic acid extraction device, comprising:
a sample extraction substrate having at least one sample collection well extending through the sample extraction substrate in a selected direction and forming a first opening and a second opening in a surface of the sample extraction substrate;
the enrichment pad is at least partially arranged in the sample collecting hole and is attached to the inner wall of the sample collecting hole, the enrichment pad and the sample collecting hole are enclosed to form a containing chamber capable of containing cell lysate, and the enrichment pad can enable liquid in the cell lysate to permeate through to retain nucleic acid in the cell lysate;
and the first water absorption pad is detachably combined with the sample extraction substrate, and can contact with the enrichment pad and absorb redundant liquid on the enrichment pad and liquid remained in the containing chamber when the first water absorption pad is close to and combined with the sample extraction substrate.
2. The nucleic acid extraction apparatus according to claim 1, characterized in that: the enrichment pad is separably matched with the sample collecting hole, and the enrichment pad can be separated from the sample collecting hole under the action of external force.
3. The nucleic acid extraction apparatus according to claim 2, characterized in that: the inner diameter of the first opening of the sample collection hole is larger than the inner diameter of the second opening, the maximum outer diameter of the enrichment pad is larger than the inner diameter of the second opening and smaller than the inner diameter of the first opening, and the enrichment pad is limited to be capable of being pulled out from the first opening only; and/or the sample collection well is a tapered well.
4. The nucleic acid extraction apparatus according to claim 3, characterized in that: the enrichment pad is of a conical structure matched with the sample collection hole; and/or the enrichment pad is of a truncated cone-shaped structure.
5. The nucleic acid extraction apparatus according to claim 3, characterized in that: the entirety of the enrichment pad is disposed within the sample collection well; and/or at least a partial area of the surface of the enrichment pad is exposed from the second opening, and the surface of the enrichment pad is flush with the plane where the second opening is located, or the whole enrichment pad is located on one side, close to the first opening, of the plane where the second opening is located.
6. The nucleic acid extraction apparatus according to claim 5, characterized in that: still be provided with protruding structure on the first water absorption pad, protruding structure can stretch into from the second opening sample acquisition is downthehole and with enrichment pad contact.
7. The nucleic acid extraction apparatus according to claim 5, characterized in that: the sample collection hole is internally provided with a second water absorption pad, the second water absorption pad is arranged on one side, close to a second opening, of the enrichment pad along the axial direction of the sample collection hole, the second water absorption pad is in contact with the enrichment pad, the surface of the second water absorption pad is flush with the plane where the second opening is located, or the part of the second water absorption pad is exposed outside the sample collection hole from the second opening and is in contact with the first water absorption pad.
8. The nucleic acid extraction apparatus according to claim 3, characterized in that: a portion of the enrichment pad is disposed within the sample collection well and another portion protrudes out of the sample collection well from the second opening.
9. The nucleic acid extraction apparatus according to any one of claims 1 to 8, characterized in that: the sample collection hole is also internally provided with a third water absorption pad, and the third water absorption pad is arranged on one side, close to the first opening, of the enrichment pad along the axial direction of the sample collection hole.
10. The nucleic acid extraction apparatus according to claim 1, characterized in that: the sample extraction matrix is internally provided with a plurality of sample collection holes, and the first water absorption pad can simultaneously cover the plurality of sample collection holes and is in contact with a plurality of enrichment pads positioned in the sample collection holes; and/or the enrichment pad is a membrane or paper-based card that can bind nucleic acids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211631536.4A CN115786078A (en) | 2022-12-13 | 2022-12-13 | Nucleic acid extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211631536.4A CN115786078A (en) | 2022-12-13 | 2022-12-13 | Nucleic acid extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115786078A true CN115786078A (en) | 2023-03-14 |
Family
ID=85425740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211631536.4A Pending CN115786078A (en) | 2022-12-13 | 2022-12-13 | Nucleic acid extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115786078A (en) |
-
2022
- 2022-12-13 CN CN202211631536.4A patent/CN115786078A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6735276B2 (en) | System and method for collecting nucleic acid samples | |
| US8062846B2 (en) | Apparatus for isolating a nucleic acid from a sample | |
| KR102037411B1 (en) | Dna extraction device based on lateral flow separation | |
| EP3112473B1 (en) | Fluid sampling device and process and kit for testing blood | |
| US20050252820A1 (en) | Sampling kits, devices and uses thereof | |
| JPWO2011004653A1 (en) | Nucleic acid extraction kit, nucleic acid extraction method and nucleic acid extraction apparatus | |
| CN115867205A (en) | Sampling device | |
| US20040245163A1 (en) | Purification device for ribonucleic acid in large volumes, and method | |
| EP3734293A1 (en) | Piston of nucleic acid extracting cartridge | |
| US20130017545A1 (en) | Apparatus and methods for acquiring analytes from a dried biological fluid sample | |
| US20090081772A1 (en) | Method and device for collecting cellular material from cells isolated on a filter | |
| CN113817601A (en) | Sample treatment and detection device | |
| WO2014048263A1 (en) | Method for pressure extraction of nucleic acids and device thereof | |
| US8110397B2 (en) | Sample preparation apparatus | |
| KR102177634B1 (en) | A preprocessing kit for molecular diagnosis | |
| CN115820412A (en) | Microfluidic card box and method for specifically detecting nucleic acid from complex sample | |
| US10473566B2 (en) | Blood sampling, storage and treatment apparatus | |
| CN219044930U (en) | Nucleic acid extraction device | |
| CN115786078A (en) | Nucleic acid extraction device | |
| CN204607994U (en) | A kind of nucleic acid rapid extraction device | |
| Manousi et al. | Fabric Phase Sorptive Extraction in Analytical Toxicology | |
| CN211771346U (en) | Magnetic rod sleeve for nucleic acid extraction | |
| CN215328142U (en) | Sample adding plate for extracting nucleic acid by magnetic bead method | |
| EP4173707A1 (en) | Device for collecting biological samples | |
| CN111876312A (en) | Nucleic acid extraction kit and nucleic acid extraction method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |