CN211771346U

CN211771346U - Magnetic rod sleeve for nucleic acid extraction

Info

Publication number: CN211771346U
Application number: CN202020145000.1U
Authority: CN
Inventors: 王群; 郑小龙; 房保海; 于业锋; 孙明君
Original assignee: Qingdao Customs Technology Center
Current assignee: Qingdao Customs Technology Center
Priority date: 2020-01-22
Filing date: 2020-01-22
Publication date: 2020-10-27
Anticipated expiration: 2030-01-22

Abstract

The utility model discloses a magnetic rod sleeve pipe for nucleic acid extraction, which comprises a fixing frame, sleeve and bar magnet, the top of mount is fixed with a plurality of bosss, the through-hole with sleeve looks adaptation is seted up at the middle part of boss, muffjoint is in the through-hole, telescopic inside is equipped with the bar magnet, the top of bar magnet is fixed with the spacing collar, and the diameter of spacing collar is greater than telescopic diameter, telescopic top is fixed with the retainer plate, and the diameter of retainer plate is greater than the diameter of through-hole, the outside of retainer plate is fixed with gear two, a side of gear two is equipped with gear one with gear two phase adaptation, gear one's bottom mounting has the pivot. The utility model discloses simple structure can play the stirring effect to solution to can wash attached to on the nucleic acid on magnetic bead surface adnexed protein, only keep the nucleic acid absorption on the magnetic bead surface, ensure abluent efficiency.

Description

Magnetic rod sleeve for nucleic acid extraction

Technical Field

The utility model relates to a nucleic acid extraction technical field particularly, relates to a magnetic rod sleeve pipe for nucleic acid extraction.

Background

The method has important effects on extracting cells and bioactive substances such as protein, nucleic acid and the like in various fields such as modern molecular biology research, clinical disease diagnosis, blood transfusion, food safety detection and the like. Among them, a bioactive substance compound in which nucleic acid is polymerized from many nucleotides is one of the most basic substances of life. Nucleic acids are widely present in all animals, plant cells, microorganisms, and organisms. Nucleic acids can be classified into ribonucleic acids (RNAs) and deoxyribonucleic acids (DNAs) according to their chemical composition. The magnetic rod method extraction method is a technology developed in recent years, and the principle is that a magnetic separation device is immersed into liquid, and magnetically attracted particles in a mixture are concentrated on the surface of the separation device under the action of a magnetic field.

SUMMERY OF THE UTILITY MODEL

The technical task of the utility model is to provide a magnetic rod sleeve for nucleic acid extraction to above not enough, solve above-mentioned problem.

The technical scheme of the utility model is realized like this:

a magnetic rod sleeve for nucleic acid extraction comprises a fixing frame, a sleeve and a magnetic rod, wherein a plurality of bosses are fixed at the top end of the fixing frame, through holes matched with the sleeve are formed in the middles of the bosses, the sleeve is connected in the through holes, the magnetic rod is arranged in the sleeve, a limiting ring is fixed at the top end of the magnetic rod, the diameter of the limiting ring is larger than that of the sleeve, a fixing ring is fixed at the top end of the sleeve, the diameter of the fixing ring is larger than that of the through holes, a second gear is fixed on the outer side of the fixing ring, a first gear matched with the second gear is arranged on one side edge of the second gear, a rotating shaft is fixed at the bottom end of the first gear, the bottom end of the rotating shaft penetrates through the bosses and extends into the bosses, the bottom end of the rotating shaft is connected with an output shaft of a motor, and accommodating cavities are fixed in positions corresponding to the bosses and the motor, the button cell is fixed in the accommodating cavity, the button cell is electrically connected with the motor, connecting rods are symmetrically fixed on two side edges of the bottom end of the fixing ring, sliding columns are fixed at the bottom ends of the connecting rods, sliding grooves matched with the sliding columns are formed in the positions, corresponding to the sliding columns, of the surfaces of the bosses, and soft iron is fixed at the bottom end of the inner portion of the sleeve.

Preferably, the bottom end of the sleeve is of a conical structure, and a plurality of grooves are uniformly formed in the outer surface of the bottom end of the sleeve.

Preferably, a limiting column is symmetrically fixed on the outer side of the magnetic rod.

Preferably, the two side edges of the interior of the sleeve are provided with limit grooves matched with the limit columns.

Preferably, a clamping cover is movably connected to the position, corresponding to the button cell, of the outer side of the boss, and one side edge of the clamping cover is movably connected with the side edge of the boss through a movable shaft.

Preferably, a control button is fixed on one side of the outer portion of the fixing frame, and the control button is electrically connected with the motor.

Compared with the prior art, the utility model discloses an advantage lies in with positive effect:

1. the utility model discloses simple structure can play the stirring effect to solution to can wash attached to on the nucleic acid on magnetic bead surface adnexed protein, only keep the nucleic acid absorption on the magnetic bead surface, ensure abluent efficiency.

2. The contact area between the groove through the bottom can be increased and the magnetic bead, the magnetic bead can be prevented from dropping, and meanwhile, the magnetic bead can not climb onto the liquid surface of the test tube.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural view of a magnetic rod casing for nucleic acid extraction according to an embodiment of the present invention;

fig. 2 is a top view of a mount according to an embodiment of the present invention;

fig. 3 is a schematic structural view of the interior of a sleeve according to an embodiment of the invention;

fig. 4 is a schematic structural view of the boss internal accommodation cavity according to the embodiment of the present invention.

In the figure:

1. a fixed mount; 2. a sleeve; 3. a magnetic bar; 4. a boss; 5. a through hole; 6. a limiting ring; 7. a stationary ring; 8. a second gear; 9. a first gear; 10. a rotating shaft; 11. a motor; 12. an accommodating chamber; 13. a button cell; 14. a connecting rod; 15. a traveler; 16. a chute; 17. soft iron; 18. a groove; 19. a limiting column; 20. a limiting groove; 21. a cover is clamped; 22. and a control button.

Detailed Description

In order to make the above objects, features and advantages of the present invention more clearly understood, the present invention will be further described with reference to the accompanying drawings and examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.

The invention will be further explained with reference to the drawings and the specific embodiments.

Embodiment one, as shown in fig. 1 to 4, a magnetic rod sleeve for nucleic acid extraction according to an embodiment of the present invention includes a fixing frame 1, a sleeve 2 and a magnetic rod 3, wherein a plurality of bosses 4 are fixed on a top end of the fixing frame 1, a through hole 5 adapted to the sleeve 2 is opened in a middle portion of the boss 4, the sleeve 2 is connected in the through hole 5, the magnetic rod 3 is arranged inside the sleeve 2, a limit ring 6 is fixed on a top end of the magnetic rod 3, a diameter of the limit ring 6 is larger than a diameter of the sleeve 2, a fixing ring 7 is fixed on a top end of the sleeve 2, a diameter of the fixing ring 7 is larger than a diameter of the through hole 5, a second gear 8 is fixed on an outer side of the fixing ring 7, a first gear 9 adapted to the second gear 8 is arranged on one side of the second gear 8, a rotating shaft 10 is fixed on a bottom end of the first gear 9, the bottom end of the rotating shaft 10 penetrates through the boss 4 and extends to the inside of the boss 4, the bottom end of the rotating shaft 10 is connected with an output shaft of the motor 11, a containing cavity 12 is fixed in the boss 4 and in a position corresponding to the motor 11, a button battery 13 is fixed in the containing cavity 12, the button battery 13 is electrically connected with the motor 11, connecting rods 14 are symmetrically fixed on two side edges of the bottom end of the fixing ring 7, sliding columns 15 are fixed at the bottom ends of the connecting rods 14, sliding grooves 16 matched with the sliding columns 15 are formed in the surface of the boss 4 and in a position corresponding to the sliding columns 15, and soft iron 17 is fixed at the bottom end of the inside of the sleeve 2.

In the second embodiment, as shown in fig. 1 and 3, the bottom end of the sleeve 2 is a tapered structure, and a plurality of grooves 18 are uniformly formed on the outer surface of the bottom end of the sleeve 2; the contact area with the magnetic beads can be increased by the tapered structure and the groove 18.

In the third embodiment, as shown in fig. 1, a limiting column 19 is symmetrically fixed on the outer side of the magnetic rod 3; the contact between the magnetic rod 3 and the sleeve 2 is more compact through the limiting column 19.

In the fourth embodiment, as shown in fig. 3, two side edges inside the sleeve 2 are provided with a limiting groove 20 adapted to the limiting post 19; the connection between the magnetic rod 3 and the sleeve 2 can be facilitated through the limiting groove 20.

In the fifth embodiment, as shown in fig. 4, a card cover 21 is movably connected to a position, corresponding to the button battery 13, outside the boss 4, and one side of the card cover 21 is movably connected to the side of the boss 4 through a movable shaft; the button cell 13 inside can be replaced conveniently by the card cover 21.

In a sixth embodiment, as shown in fig. 1, a control button 22 is fixed on one side of the exterior of the fixing frame 1, and the control button 22 is electrically connected to the motor 11; the control button 22 can control the rotation of the motor 11 conveniently, thereby facilitating the operation and control of people.

For the convenience of understanding the technical solution of the present invention, the following detailed description is made on the working principle or the operation mode of the present invention in the practical process.

In the time of practical application, through the inside with bar magnet 3 inserts sleeve 2, thereby make sleeve 2 have magnetic force, thereby can play the adsorption to the magnetic bead in the solution, control button 22 through the outside can make things convenient for the rotation of control motor 11, the rotation of motor 11 drives the rotation of pivot 10, the rotation of pivot 10 drives the rotation of gear 9, thereby drive the rotation of gear two 8 and sleeve 2 through the rotation of gear 9, can mix the stirring to the solution through the rotation of sleeve 2, thereby can improve and abluent efficiency, soft iron 17 through the inside bottom of sleeve 2 makes when pulling out bar magnet 3, the magnetic force in the sleeve 2 can not disappear, the recess 18 of sleeve 2 bottom can increase the area of contact with the magnetic bead simultaneously, thereby can be more even adsorb the magnetic bead at once, improve absorbent efficiency.

Through the above detailed description, the person skilled in the art can easily realize the present invention. It should be understood, however, that the intention is not to limit the invention to the particular embodiments described. On the basis of the disclosed embodiments, a person skilled in the art can combine different technical features at will, thereby implementing different technical solutions.

Claims

1. The magnetic rod sleeve for nucleic acid extraction is characterized by comprising a fixing frame (1), a sleeve (2) and a magnetic rod (3), wherein a plurality of bosses (4) are fixed at the top end of the fixing frame (1), through holes (5) matched with the sleeve (2) are formed in the middle of the bosses (4), the sleeve (2) is connected in the through holes (5), the magnetic rod (3) is arranged inside the sleeve (2), a limiting ring (6) is fixed at the top end of the magnetic rod (3), the diameter of the limiting ring (6) is larger than that of the sleeve (2), a fixing ring (7) is fixed at the top end of the sleeve (2), the diameter of the fixing ring (7) is larger than that of the through holes (5), a second gear (8) is fixed on the outer side of the fixing ring (7), a first gear (9) matched with the second gear (8) is arranged on one side edge of the second gear (8), a rotating shaft (10) is fixed at the bottom end of the first gear (9), the bottom end of the rotating shaft (10) penetrates through the boss (4) and extends into the boss (4), the bottom end of the rotating shaft (10) is connected with an output shaft of a motor (11), a containing cavity (12) is fixed in the boss (4) at a position corresponding to the motor (11), a button cell (13) is fixed in the containing cavity (12), the button cell (13) is electrically connected with the motor (11), the two side edges of the bottom end of the fixing ring (7) are symmetrically fixed with connecting rods (14), a sliding column (15) is fixed at the bottom end of the connecting rod (14), a sliding groove (16) matched with the sliding column (15) is formed in the surface of the boss (4) and the position corresponding to the sliding column (15), and soft iron (17) is fixed at the bottom end inside the sleeve (2).

2. The magnetic rod sleeve for nucleic acid extraction according to claim 1, wherein the bottom end of the sleeve (2) is of a conical structure, and a plurality of grooves (18) are uniformly formed on the outer surface of the bottom end of the sleeve (2).

3. The magnetic rod sleeve for nucleic acid isolation according to claim 1, wherein a stopper (19) is symmetrically fixed to the outside of the magnetic rod (3).

4. The magnetic rod sleeve for nucleic acid extraction according to claim 3, wherein the two sides of the interior of the sleeve (2) are provided with a limiting groove (20) adapted to the limiting column (19).

5. The magnetic rod sleeve for nucleic acid extraction according to claim 1, wherein a clamp cover (21) is movably connected to a position on the outer side of the boss (4) corresponding to the button cell (13), and one side of the clamp cover (21) is movably connected to the side of the boss (4) through a movable shaft.

6. The magnetic rod sleeve for nucleic acid extraction according to claim 1, wherein a control button (22) is fixed to one side of the exterior of the holder (1), and the control button (22) is electrically connected to the motor (11).