CN201406430Y - Online coli-group fast detecting device - Google Patents
Online coli-group fast detecting device Download PDFInfo
- Publication number
- CN201406430Y CN201406430Y CN2009201519941U CN200920151994U CN201406430Y CN 201406430 Y CN201406430 Y CN 201406430Y CN 2009201519941 U CN2009201519941 U CN 2009201519941U CN 200920151994 U CN200920151994 U CN 200920151994U CN 201406430 Y CN201406430 Y CN 201406430Y
- Authority
- CN
- China
- Prior art keywords
- coliform
- retaining plate
- sampler
- translation
- detection device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Abstract
The utility model provides an online coli-group fast detecting device with the advantages of simple operating structure, convenient use, and suitability for online detection. The technical scheme of the utility model is as follows: a mounting plate is included, a thermostat and a translation table support, a right-angle fixing plate is arranged above the translation table support, a lateral translation table is arranged on the translation table support, two vertical translation tables are respectively arranged on the lateral translation table, a piston fixing plate and a sample injector fixingplate are respectively arranged on the two vertical translation plates, a sample injector and a vacuum chuck device are respectively mounted on the sample injector fixing plate, and the piston fixingplate, the sample injector and the vacuum chuck device are respectively arranged above the thermostat.
Description
Technical field
The utility model belongs to coliform proofing unit field, and especially a kind of coliform on-line quick detection device of simple to operate, easy to use and suitable online detection can be monitored coliform and excrement colibacillus group in the water quality simultaneously.
Background technology
Coliform means that a group cultivates the Gram-negative sporeless bacterium that 24～48 hours energy ferment lactosees produce sour aerogenesis at 37 ℃.The coliform that only derives from people and warm-blooded animal ight soil claims excrement colibacillus group, is the heat-resisting coliform that can grow under 44.5 ℃ of culture temperature.China carries out hygienical evaluation to Drinking Water, source water, surface water etc. at present, and the index of detection is coliform and excrement colibacillus group or colon bacillus, with the indicator of these indexs as fecal pollution.The accepted standard detection method is membrane filter method and multitube fermentation method.But these two kinds of methods are longer relatively detection time, need 48-72 hour, need proof test, and testing sequence is comparatively loaded down with trivial details, can not make quick evaluation to the hygiology situation of water.Therefore, adopt fast and convenient detection method very necessary.The enzyme substrates method can remedy the deficiency of traditional method preferably.
At present, test set has the online microorganism monitoring instrument of (1) Colifast CALM, and it can provide the data of thermotolerance coliform, intestinal bacteria and total coli group in the water sample, and its detection principle is that selective medium and automatic analyser are combined.Contain fluorogenic substrate in the substratum, produced fluorescence, further detect by the Colifast analyser by the specific enzymes hydrolysis of coliform.The problem that exists is a complicated operation; (2) TOGSTM 9000 intestinal bacteria (quantitatively) determinator, it has increased new function on Aquasure TMPRO 3000 bases, adopt fluorophotometer, colourimeter, turbidometer that sample is carried out quantitative analysis.Shortcoming is can not carry out on-line continuous to detect.
The utility model content
The purpose of this utility model provides the coliform on-line quick detection device of simple, the easy to use and suitable online detection of a kind of operating structure.
The technical solution of the utility model is: coliform on-line quick detection device, comprise mounting plate, it is characterized in that mounting plate is provided with thermostat container and translation stage support, be provided with the right angle retaining plate above the translation stage support, the translation stage support is provided with the transverse translation platform, the transverse translation platform is provided with vertical translation platform and vertical translation platform, be respectively arranged with piston retaining plate and sampler retaining plate on the two vertical translation platforms, sampler and vacuum chuck device, piston retaining plate have been installed respectively again on the sampler retaining plate, sampler and vacuum chuck device lay respectively at thermostat container above.
Described thermostat container comprises that can be set the also casing of controlled temperature, be provided with insulation can in the intravital bottom of case base plate is installed, insulation can is installed base plate and is provided with electric rotary table, is provided with rotating disk above the electric rotary table, and the upper end of rotating disk is provided with photometer.
The described groove that culturing bottle is installed that is provided with on rotating disk is placed with the culturing bottle of testing usefulness in the groove the inside.
Described culturing bottle is aseptic, the colourless transparent glass bottle of band plug and aluminium cover.
Effect of the present utility model is: coliform on-line quick detection device, comprise mounting plate, mounting plate is provided with thermostat container and translation stage support, be provided with the right angle retaining plate above the translation stage support, the translation stage support is provided with the transverse translation platform, the transverse translation platform is provided with vertical translation platform and vertical translation platform, be respectively arranged with piston retaining plate and sampler retaining plate on the two vertical translation platforms, sampler and vacuum chuck device, piston retaining plate have been installed respectively again on the sampler retaining plate, sampler and vacuum chuck device lay respectively at thermostat container above.Have following characteristics: (1) is easy and simple to handle, does not need complexity, expensive experimental chamber equipment and professional to get final product on-the-spot test; (2) can judge coliform and excrement colibacillus group in the water sample simultaneously; (3) short of sense cycle needs 2～12 hours (analysis speed is fast); (4) but the high-throughput continuous detecting; (5) really accomplish real-time, online, rapid detection.
Test bottle is equipped with the excrement colibacillus group test fluid for aseptic, the colourless transparent glass bottle of band plug and aluminium cover in the test bottle.Pre-configured test fluid is packaged in the clear-glass bottle, can be directly used in the mensuration of bacterium, carry, easy to use, measure simple fast, accurately, the shelf time is long.Its form can be expanded and be used for the microorganism detection that other a plurality of microorganism projects are used for a plurality of fields such as water quality, food, medicine, makeup.Utilize the bottled excrement colibacillus group test fluid of test to have following characteristics: (1) substratum is used for microorganism detection with bottled form, is a kind of brand-new form; (2) bottled substratum must use in conjunction with photometer, and testing process draws corresponding sample concentration by the variation of METHOD FOR CONTINUOUS DETERMINATION sample liquid absorbancy, and its testing process is more simple, quick; (3) this bottled substratum can the directly disposable detection that is used for on-line instrument, does not need the professional promptly to can be used for on-line instrument; Also need not on operating environment of making substratum and equipment, to drop into substantial contribution; (4) come the splendid attire substratum with the glass culturing bottle, the preparation and the sterilising treatment link that do not need early stage have been saved the early-stage preparations time of test sample, are equivalent to shorten entire sample detection time; (5) be used for microorganism detection in conjunction with online detecting instrument, can increase work efficiency greatly, alleviate the workload of checking the staff, simple and easy to do, reliable; (6) easy to carry, use simple, the shelf time is long.
Below in conjunction with drawings and Examples the utility model is described further.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is the left view of Fig. 1;
Fig. 3 is the vertical view of Fig. 1.
Embodiment
Among Fig. 1, coliform on-line quick detection device, comprise mounting plate 21, mounting plate 21 is provided with thermostat container 8 and translation stage support 18, be provided with right angle retaining plate 19 above the translation stage support 18, translation stage support 18 is provided with transverse translation platform 3, transverse translation platform 3 is provided with two vertical translation platforms 1, vertical translation platform 2, wherein be respectively arranged with piston retaining plate 13 and sampler retaining plate 14 on the two vertical translation platforms 1, sampler 15 has been installed respectively again on the sampler retaining plate 14, vacuum chuck device 16, piston retaining plate 13 and sampler 15, vacuum chuck device 16 lays respectively at the top (referring to Fig. 2 of thermostat container, Fig. 3).
Be provided with the groove that culturing bottle is installed on rotating disk 7, the culturing bottle 20 of test usefulness has been placed in these groove the insides, and culturing bottle is aseptic, the colourless transparent glass bottle of band plug and aluminium cover.
Principle of work of the present utility model is: the foundation of enzyme-substrate method is that coliform produces a kind of specific enzymes.The substrate that native system adopts can be discharged a kind of colored compound by this specific enzymes decomposition, under solution state, in the finite concentration scope, meets Law of Lambert-Beer.According to this principle, the absorbancy of certain wavelength of the solution by surveying enzyme reaction product can corresponding flora concentration.Temperature by flora is cultivated in control can realize the detection to coliform and excrement colibacillus group.
Structurally, adopt mechanical manipulator and rotating disk to combine.During use, utilize computer control,, carry out continuous sampling by the sampling pattern of predetermined set.
Before detecting, water sample to be measured is filtered and concentration, adopt tangential flow ultrafilter, water sample is filtered, utilize membrane separation technique that water sample is concentrated simultaneously, can at short notice water sample be concentrated 10 times, can significantly reduce detection time like this.
Test cell adopts two light source monochromatic light journeys that water sample is detected.At first nutrient solution is carried out rapid previewing with 600nm ruddiness, getting rid of assorted bacterium disturbs, use certain wavelength UV-light Continuous irradiation nutrient solution then, solution absorbency and flora quantity are followed langbobier law, the data that system is recorded are delivered to data Collection ﹠ Processing System and are calculated flora quantity then, show and print various parameters, chart and transfer to environmental management department by data transmission system.The real realization sends the report fully automated from sampling to detect, and accomplishes that the on-line continuous of water quality detects and system remote control.
Culturing bottle is set by the vacuum slot follow procedure and is put into constant incubator, is placed on the concrete bottle position of the specified electric rotary table of program, after sample cultivation finishes, is set by the vacuum slot follow procedure and takes out in constant incubator, unified again recycling.
Claims (4)
1, coliform on-line quick detection device, comprise mounting plate (21), it is characterized in that mounting plate (21) is provided with thermostat container (8) and translation stage support (18), be provided with right angle retaining plate (19) above the translation stage support (18), translation stage support (18) is provided with transverse translation platform (3), transverse translation platform 3 is provided with vertical translation platform (1) and vertical translation platform (2), be respectively arranged with piston retaining plate (13) and sampler retaining plate (14) on the two vertical translation platforms, sampler (15) and vacuum chuck device (16) have been installed, piston retaining plate (13) on the sampler retaining plate (14) again respectively, sampler (15) and vacuum chuck device (16) lay respectively at thermostat container above.
2, coliform on-line quick detection device according to claim 1, it is characterized in that described thermostat container (8) comprises that can be set the also casing of controlled temperature, be provided with insulation can in the intravital bottom of case base plate (9) is installed, insulation can is installed base plate (9) and is provided with electric rotary table (10), be provided with rotating disk (7) above the electric rotary table (10), the upper end of rotating disk (7) is provided with photometer (6).
3, coliform on-line quick detection device according to claim 2 is characterized in that the described groove that culturing bottle is installed that is provided with on rotating disk (7), be placed with the culturing bottle (20) of test usefulness in the groove the inside.
4, coliform on-line quick detection device according to claim 3 is characterized in that aseptic, the colourless transparent glass bottle of described culturing bottle (20) for band plug and aluminium cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201519941U CN201406430Y (en) | 2009-05-07 | 2009-05-07 | Online coli-group fast detecting device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201519941U CN201406430Y (en) | 2009-05-07 | 2009-05-07 | Online coli-group fast detecting device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201406430Y true CN201406430Y (en) | 2010-02-17 |
Family
ID=41677362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201519941U Expired - Lifetime CN201406430Y (en) | 2009-05-07 | 2009-05-07 | Online coli-group fast detecting device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201406430Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175632A (en) * | 2010-12-31 | 2011-09-07 | 聚光科技(杭州)股份有限公司 | Method and device for detecting bacteria |
| CN103025415A (en) * | 2010-07-22 | 2013-04-03 | 生物梅里埃有限公司 | Belt agitation system for culture containers |
| CN103837349A (en) * | 2012-11-20 | 2014-06-04 | 苏州工业园区高登威科技有限公司 | Installation method for supporting device of temperature control box |
| CN112063521A (en) * | 2020-09-16 | 2020-12-11 | 杭州绿洁环境科技股份有限公司 | Coliform group on-line measuring system |
-
2009
- 2009-05-07 CN CN2009201519941U patent/CN201406430Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103025415A (en) * | 2010-07-22 | 2013-04-03 | 生物梅里埃有限公司 | Belt agitation system for culture containers |
| CN103025415B (en) * | 2010-07-22 | 2016-01-20 | 生物梅里埃有限公司 | For the band agitation system of culture vessel |
| CN102175632A (en) * | 2010-12-31 | 2011-09-07 | 聚光科技(杭州)股份有限公司 | Method and device for detecting bacteria |
| CN103837349A (en) * | 2012-11-20 | 2014-06-04 | 苏州工业园区高登威科技有限公司 | Installation method for supporting device of temperature control box |
| CN112063521A (en) * | 2020-09-16 | 2020-12-11 | 杭州绿洁环境科技股份有限公司 | Coliform group on-line measuring system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101893589B (en) | Sterility test method and totally closed bacteria collection ampoule incubator used thereby | |
| CN102757891B (en) | Automatic microbial inoculation instrument | |
| CN108459051B (en) | A kind of device and application method automatically recording Escherichia coli Growth curve | |
| CN201406430Y (en) | Online coli-group fast detecting device | |
| CN104374758B (en) | Method for determining oleaginous microalgae harvesting time by utilizing chlorophyll fluorescence parameter Pv/Fm | |
| CN101113985B (en) | Sanitary status on-site rapid detection device and detecting method | |
| US20040009572A1 (en) | Apparatus for the analysis of microorganisms growth and procedure for the quantification of microorganisms concentration | |
| CN101319248A (en) | Method for directly preparing microorganism testing slice with commercial product culture medium dry powder | |
| CN201707297U (en) | Escherichia coli fluorescence fast detection system | |
| CN204964400U (en) | Detect total plate count and coliform's quick detecting system of multichannel simultaneously | |
| CN203474788U (en) | Food microbial detection kit | |
| CN103196841A (en) | Method for analyzing succinic acid fermentation liquid by utilizing near infrared spectrums on line | |
| CN106867890A (en) | A kind of microdisk electrode Optimal Control System and method | |
| CN101943660A (en) | Method for detecting psychrophilic bacteria in crude milk by utilizing aminopeptidase method | |
| CN202649201U (en) | Water coli group online automatic detection system | |
| CN107764754A (en) | A kind of online test method of microbes biomass | |
| CN203587502U (en) | Multi-parameter micro culture detection device | |
| CN106872341A (en) | A kind of instant microbe diagnosis instrument of movement based on smart mobile phone | |
| CN202450092U (en) | System for monitoring oxygen content and physiological activity of microalgae in closed photobioreactor | |
| CN209685760U (en) | A kind of novel foodstuff Micro biological Tests workbench | |
| CN106635782A (en) | Oleaginous microalgae integrated culture reactor and culture method | |
| CN203333648U (en) | Separate culture device for biological samples | |
| CN203117162U (en) | Integrated membrane biosensor for quickly detecting escherichia coli | |
| CN206671203U (en) | A kind of detection box for being used to detect multiple-microorganism in food | |
| CN111455017A (en) | Method for measuring total number of microbial colonies in food |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: QINGDAO JIAMING MEASUREMENT AND CONTROL INSTRUMENT Free format text: FORMER OWNER: QINGDAO JIAMING ENVIRONMENTAL EQUIPMENT CO., LTD. Effective date: 20121101 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20121101 Address after: 266000 Shandong city of Qingdao province Fuzhou Northroad 169-919 Patentee after: QINGDAO JIAMING MEASUREMENT AND CONTROL TECHNOLOGY CO., LTD. Address before: 266000 Shandong city of Qingdao province Fuzhou Northroad 169-919 Patentee before: Qingdao Jiaming Environmental Equipment Co., Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20100217 |